CN109618851B - Kudzu seedling method - Google Patents
Kudzu seedling method Download PDFInfo
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- CN109618851B CN109618851B CN201811561316.2A CN201811561316A CN109618851B CN 109618851 B CN109618851 B CN 109618851B CN 201811561316 A CN201811561316 A CN 201811561316A CN 109618851 B CN109618851 B CN 109618851B
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention provides a method for breeding kudzuvine roots, which relates to the technical field of traditional Chinese medicinal material cultivation, and comprises a sprout promoter preparation stage and a seedling breeding stage, wherein the sprout promoter preparation stage comprises the following steps: carrying out strain amplification culture, preparing a supernatant, preparing a chelating solution and preparing a bud promoting agent; the seedling stage comprises the following steps: obtaining culture seeds, pretreating a seedling raising field, raising seedlings by cutting and managing the seedlings; the kudzu seedling method is suitable for cutting seedlings and tissue culture seedlings, solves the problems of the two existing seedling methods, and the kudzu seedlings produced by the kudzu seedling method are healthy and strong in seedling body and strong in resistance, lays a solid foundation for high yield of the current year, and is convenient, simple and easy to operate and environment-friendly in the seedling process.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of traditional Chinese medicine cultivation, in particular to a method for raising seedlings of kudzuvine roots.
[ background of the invention ]
Wild radix Puerariae is root tuber of Pueraria lobata or Pueraria thomsonii of Leguminosae, is a common vegetable in some provinces of south China, is sweet, cool and delicious, and can be used as soup or medicine.
The traditional kudzuvine root seedling culture is cutting seedling culture. Generally, after underground root tubers are harvested, the upper potato vines are cut into small segments and are subjected to cuttage seedling culture. The seedling growing time is generally 1 in the middle and last ten days of the month, and about 3 months in the next year. Long time and low germination rate. In recent years, in order to improve the yield, paclobutrazol is largely used to control the growth amount of overground parts, and the germination rate and rooting rate of the pueraria lobata cuttage are lower and lower. The germination and rooting rate of some farmers is less than 50 percent.
The kudzu root has tissue culture seedlings besides the traditional cutting seedlings. Because the tissue culture seedling is weak, facility seedling is needed. The technical difficulty of the kudzu root tissue culture seedling culture is high, because the seedling culture is started in about 12 months generally when the farm is driven to the field, normally about 80-90 days are needed, the cost is high, and the time is long.
The invention aims at researching a kudzuvine root seedling culture method which saves the production cost and improves the yield.
[ summary of the invention ]
In view of the above, the invention aims to provide a kudzu seedling raising method, which is simultaneously suitable for cutting seedlings and tissue culture seedlings and solves the problems of the two existing seedling raising methods.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for raising seedlings of kudzuvine roots comprises the following steps:
first, preparing bud promoting agent
(1) And (3) strain amplification culture: mixing brown sugar and wheat bran according to a mass ratio of 1: mixing according to the proportion of 10-11, inoculating 1-2mL of probiotics, adding water with the same volume as the probiotics, and naturally fermenting for 24-25 hours to obtain a strain for later use;
(2) preparing a supernatant fluid: mixing the powder of the kudzu, the pineapple and the cedar wood chips according to the mass ratio of 1-2:2:1, then mixing the obtained mixture with the strain obtained in the step (1) according to the mass ratio of 10-11:1, putting the mixture into a tank, sealing and fermenting for 90-95 days, and filtering the fermented product to obtain a supernatant for later use;
(3) preparing a chelating solution: a. firstly, dissolving sodium selenite in water to obtain 130g/L sodium selenite aqueous solution with the concentration of 120-; b. mixing the mother liquor obtained in the step a with an amino acid solution with the mass concentration of 20-30%, and then carrying out oscillation chelation for 48-72 hours at 35-45 ℃ to obtain a chelating solution for later use;
(4) preparing a bud promoting agent: mixing the supernatant obtained in the step (2) with the chelating liquid obtained in the step (3) according to the volume ratio of 3-4:1-2, and then adding sodium alkyl benzene sulfonate; stirring at 25-35 deg.C, chelating for 36-48 hr to obtain amino acid chelating germination promoter;
(II) seedling raising stage
1) Obtaining a cultured seed: cutting radix Puerariae into short vines with bud points and length of 8-9cm, wherein the upper part of the bud point is 1-2cm long, and the lower part is 7-8cm long, so as to avoid head and tail inversion and influence on growth;
2) pretreatment of a seedling raising field: ridging the field, wherein the ridge height is 15-20cm, and spraying carbendazim for disinfection;
3) cutting and seedling raising: diluting the amino acid chelating germination promoter obtained in the step (one) with water according to the volume ratio of 1:600-650, then soaking the brachypodium obtained in the step 1) in the diluted solution for 10-12min, taking out the brachypodium, vertically inserting the brachypodium into ridges, keeping the bud points on the soil, and completely immersing the lower parts of the bud points in the soil;
4) seedling management: after the bud point starts to sprout in 6-9 days after cuttage, spraying a solution prepared by mixing the amino acid chelating germination promoter obtained in the step (I) and water according to the proportion of 1: 1000-; and when the new buds grow to 3-4cm and 2-3 true leaves grow, spraying a foliar fertilizer by combining intertillage weeding, and transplanting in a field when the height of the seedlings is 9-11cm and the roots are developed.
In the invention, further, the seedling stage of the step (two) comprises the following steps:
firstly, tissue culture seedling treatment: cleaning the tissue culture seedlings, soaking the tissue culture seedlings in carbendazim for 9-11min, then transplanting the tissue culture seedlings into seedling raising cups, and placing the seedling raising cups in a seedling raising shed;
seedling management: spraying water to the transplanted tissue culture seedlings in the morning and at night every day, maintaining the temperature of a seedling raising shed to be not lower than 18 ℃, the humidity to be not lower than 70%, and spraying a bactericidal agent every 6-8 days; and 3-4 days after transplanting, diluting the amino acid chelating germination promoter obtained in the step (I) with water according to the volume ratio of 1:950-1000, spraying the solution obtained after dilution on the tissue culture seedlings, and spraying foliar fertilizer by combining intertillage weeding when the new buds grow to 2-4cm and 2-3 true leaves grow out, wherein after the seedlings are 9-11cm high and the roots are developed, the field transplanting can be carried out.
In the invention, the volume ratio of the mother liquor to the amino acid solution in the step (3) is 1: 3-4.
In the invention, further, the addition amount of the sodium alkyl benzene sulfonate in the step (4) is 5-6g per liter of chelating solution.
In the invention, the foliar fertilizer is prepared from the following raw materials in parts by weight: 20-25 parts of aloe powder, 6-12 parts of brassinolide, 8-16 parts of bamboo vinegar, 5-10 parts of sugar alcohol chelated liquid fertilizer, 10-20 parts of urea and 200 parts of 120-one water.
In the invention, the bactericide in the second step is carbendazim solution diluted by 300 times by adding water.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the kudzu vine root seedling raising method is simultaneously suitable for cutting seedlings and tissue culture seedlings, solves the problems of the two existing seedling raising methods, and comprises a sprout promoter preparation stage and a seedling raising stage, wherein the sprout promoter is prepared by chelating kudzu vine root, pineapple and cedar wood chips with supernatant after fermentation of strains and then adding sodium alkyl benzene sulfonate, wherein the kudzu vine root provides a nitrogen source and multiple small molecular substances to promote cell growth, the pineapple provides vitamins, the pineapple contains a large amount of reducing sugar and cellulose to meet the needs of plant growth, the cedar wood chips provide salicylic acid, and the three are mixed, so that the seedling growth can be effectively promoted, and the seedling strengthening rate is improved; the added sodium alkyl benzene sulfonate forms a balanced stable nutrient unit, so that the balance and stability of nutrient components are ensured; the field is disinfected by the carbendazim before cuttage, the field can be sterilized by carrying frontal germs, and the produced kudzu vine root seedlings are healthy and strong in seedling bodies and strong in resistance by matching with management of a seedling period; the problems of long seedling raising time and low germination rate of the conventional cutting seedling raising are solved; in the tissue culture seedling raising process, firstly, the tissue culture seedlings are soaked and sterilized by using carbendazim, so that the disease phenomenon in the tissue culture process caused by germs carried by the tissue culture seedlings is prevented, and a bactericide, a bud promoting agent and a leaf fertilizer are sprayed on the tissue culture seedlings in a matching mode during the culture period, so that the problems in the prior tissue culture technology can be solved, the obtained kudzu root seedlings are strong, and the rooting and strengthening rate is high, and the resistance is strong; the seedling raising method disclosed by the invention adopts the bud promoting agent for treatment, not only can be used for cutting seedling raising, but also is suitable for tissue culture seedling raising, the seedling raising time is saved by about 20 days compared with the traditional seedling raising time, the kudzuvine root seedlings can land in advance, the seedlings are slowed in advance, the growth period is prolonged, and a solid foundation is laid for the high yield of the current year; and the seedling raising process is convenient, simple and easy to operate and environment-friendly.
2. In the foliar fertilizer, the aloe powder has the protection effect on cell tissues and the activation and regeneration effect on damaged cells; brassinolide can promote plant growth, improve quality, increase yield, and has bright color and thick leaves; the sugar alcohol chelated liquid fertilizer can improve the stress resistance of plants, the main component of the sugar alcohol chelated liquid fertilizer is a sugar alcohol substance, and the sugar alcohol substance is a mixture formed by mannitol, sorbitol, xylitol and glycerol and nutrient elements required by crops according to a specific proportion, the nutrient components of the sugar alcohol chelated liquid fertilizer can be easily absorbed by leaves, and after entering the interior of the leaves, the sugar alcohol chelated liquid fertilizer can carry nutrients to smoothly enter phloem for nutrient transportation; the urea is a nitrogen fertilizer with the highest nitrogen content, is suitable for various soils and plants, and can promote the division and growth of cells and promote branches and leaves to grow luxuriantly; the bamboo vinegar has good permeability and absorbability to plant and animal tissues, and can play a role in plant hormone, and the addition of a proper amount of bamboo vinegar can play a role in promoting rooting, germination and growth of radix Puerariae, so that the radix Puerariae medicinal material has better properties.
[ detailed description ] embodiments
The following examples may help one skilled in the art to more fully understand the present invention, but are not intended to limit the invention in any way.
Example 1
The method for raising the seedlings of the kudzuvine roots provided by the embodiment comprises the following steps:
(1) and (3) strain amplification culture: mixing brown sugar and wheat bran according to the mass ratio of 1:10, inoculating 1mL of probiotics, adding water with the same volume as the probiotics, and naturally fermenting for 24 hours to obtain a strain for later use;
(2) preparing a supernatant fluid: mixing the powder of the kudzu, the pineapple and the cedar wood chips according to the mass ratio of 1:2:1, then mixing the obtained mixture with the strain obtained in the step (1) according to the mass ratio of 10:1, putting the mixture into a tank, sealing and fermenting for 90 days, and filtering the fermented product to obtain a supernatant for later use;
(3) preparing a chelating solution: a. firstly, dissolving sodium selenite in water to obtain a sodium selenite aqueous solution with the concentration of 120g/L, and taking the sodium selenite aqueous solution as a mother solution for later use; b. mixing the mother liquor obtained in the step a with an amino acid solution with the mass concentration of 20%, wherein the volume ratio of the mother liquor to the amino acid solution is 1: 3; then carrying out oscillation chelation for 48 hours at 35 ℃ to obtain a chelating solution for later use;
(4) preparing a bud promoting agent: mixing the supernatant obtained in the step (2) with the chelating liquid obtained in the step (3) according to the volume ratio of 3:1, and then adding sodium alkyl benzene sulfonate, wherein the adding amount of the sodium alkyl benzene sulfonate is 5g per liter of chelating liquid; stirring at 25 ℃, and chelating for 36 hours to obtain an amino acid chelating sprouting promoter;
(II) seedling raising stage
1) Obtaining a cultured seed: cutting a kudzu vine into 8cm short vines with bud points, wherein the upper part of each bud point is 1cm long, and the lower part of each bud point is 7cm long, so that the situation that the growth is influenced due to head and tail inversion is avoided;
2) pretreatment of a seedling raising field: ridging the field, wherein the ridge height is 15cm, and spraying carbendazim for disinfection;
3) cutting and seedling raising: diluting the amino acid chelating germination promoter obtained in the step (one) with water according to the volume ratio of 1:600, then soaking the brachypodium obtained in the step 1) in the diluted solution for 10min, taking out the brachypodium, vertically inserting the brachypodium into ridges, keeping the bud points on the soil, and completely immersing the lower parts of the bud points in the soil;
4) seedling management: after the bud point starts to sprout after 6 days of cuttage, spraying a solution prepared by mixing the amino acid chelating germination promoter obtained in the step one with water according to the ratio of 1:1000 for one time; when the sprout grows to 3cm and 2 true leaves grow, spraying a foliar fertilizer by combining intertillage weeding, wherein the foliar fertilizer consists of the following raw materials in parts by weight: 20 parts of aloe powder, 6 parts of brassinolide, 8 parts of bamboo vinegar, 5 parts of sugar alcohol chelated liquid fertilizer, 10 parts of urea and 120 parts of water; and (5) transplanting the seedlings in the field after the seedlings are 9cm high and roots are developed.
Example 2
The method for raising the seedlings of the kudzuvine roots provided by the embodiment comprises the following steps:
(1) and (3) strain amplification culture: mixing brown sugar and wheat bran according to a mass ratio of 1:11, inoculating 1-2mL of probiotics, adding water with the same volume as the probiotics, and naturally fermenting for 25 hours to obtain a strain for later use;
(2) preparing a supernatant fluid: mixing the powder of the kudzu, the pineapple and the cedar wood chips according to the mass ratio of 2:2:1, then mixing the obtained mixture with the strain obtained in the step (1) according to the mass ratio of 11:1, putting the mixture into a tank, sealing and fermenting for 95 days, and filtering the fermented product to obtain a supernatant for later use;
(3) preparing a chelating solution: a. firstly, dissolving sodium selenite in water to obtain 130g/L sodium selenite aqueous solution as mother solution for later use; b. mixing the mother liquor obtained in the step a with an amino acid solution with the mass concentration of 30%, wherein the volume ratio of the mother liquor to the amino acid solution is 1: 4; then carrying out oscillation chelation for 72 hours at the temperature of 45 ℃ to obtain a chelating solution for later use;
(4) preparing a bud promoting agent: mixing the supernatant obtained in the step (2) with the chelating liquid obtained in the step (3) according to the volume ratio of 2:1, and then adding sodium alkyl benzene sulfonate, wherein the adding amount of the sodium alkyl benzene sulfonate is 6g per liter of chelating liquid; stirring at 35 ℃, and chelating for 48 hours to obtain an amino acid chelating sprouting promoter;
(II) seedling raising stage
1) Obtaining a cultured seed: cutting a kudzu vine into 9cm short vines with bud points, wherein the upper parts of the bud points are 2cm long, and the lower parts of the bud points are 7cm long, so that the situation that the growth is influenced due to head and tail inversion is avoided;
2) pretreatment of a seedling raising field: ridging the field, wherein the ridge height is 20cm, and spraying carbendazim for disinfection;
3) cutting and seedling raising: diluting the amino acid chelating germination promoter obtained in the step (one) with water according to the volume ratio of 1:650, then soaking the brachypodium obtained in the step 1) in the diluted solution for 12min, taking out the brachypodium, vertically inserting the brachypodium into ridges, keeping the bud points on the soil, and completely immersing the lower parts of the bud points in the soil;
4) seedling management: after the bud point starts to sprout after 9 days of cuttage, spraying a solution prepared by mixing the amino acid chelating germination promoter obtained in the step (I) and water according to the ratio of 1:1100 once; when the sprout grows to 4cm and 3 true leaves grow, spraying a foliar fertilizer by combining intertillage weeding, wherein the foliar fertilizer consists of the following raw materials in parts by weight: 25 parts of aloe powder, 12 parts of brassinolide, 16 parts of bamboo vinegar, 10 parts of sugar alcohol chelated liquid fertilizer, 20 parts of urea and 200 parts of water; and (5) transplanting the seedlings in the field after the seedlings are 11cm high and roots are developed.
Example 3
The method for raising the seedlings of the kudzuvine roots provided by the embodiment comprises the following steps:
(1) and (3) strain amplification culture: mixing brown sugar and wheat bran according to a mass ratio of 1:10, inoculating 1mL of probiotics, adding water with the same volume as the probiotics, and naturally fermenting for 24 hours to obtain a strain for later use;
(2) preparing a supernatant fluid: mixing the powder of the kudzu, the pineapple and the cedar wood chips according to the mass ratio of 1:2:1, then mixing the obtained mixture with the strain obtained in the step (1) according to the mass ratio of 10:1, putting the mixture into a tank, sealing and fermenting for 90 days, and filtering the fermented product to obtain a supernatant for later use;
(3) preparing a chelating solution: a. firstly, dissolving sodium selenite in water to obtain a sodium selenite aqueous solution with the concentration of 120g/L, and taking the sodium selenite aqueous solution as a mother solution for later use; b. mixing the mother liquor obtained in the step a with an amino acid solution with the mass concentration of 20%, wherein the volume ratio of the mother liquor to the amino acid solution is 1: 3; then carrying out oscillation chelation for 48 hours at 35 ℃ to obtain a chelating solution for later use;
(4) preparing a bud promoting agent: mixing the supernatant obtained in the step (2) with the chelating liquid obtained in the step (3) according to the volume ratio of 3:1, and then adding sodium alkyl benzene sulfonate, wherein the adding amount of the sodium alkyl benzene sulfonate is 5g per liter of chelating liquid; stirring at 25 ℃, and chelating for 36 hours to obtain an amino acid chelating sprouting promoter;
(II) seedling raising stage
Firstly, tissue culture seedling treatment: cleaning the tissue culture seedlings, soaking the tissue culture seedlings in carbendazim for 9min, then transplanting the tissue culture seedlings into seedling raising cups, and placing the seedling raising cups in seedling raising sheds;
seedling management: spraying water to the transplanted tissue culture seedlings in the morning and at night every day, maintaining the temperature of a seedling raising shed to be not lower than 18 ℃ and the humidity to be not lower than 70%, and spraying a sterilizing agent once every 6 days, wherein the sterilizing agent is a carbendazim solution diluted by 300 times by adding water; and (3) on the 3 rd day after transplanting, diluting the amino acid chelating germination promoter obtained in the step (I) with water according to the volume ratio of 1:950, spraying the solution obtained after dilution on the tissue culture seedlings, and spraying a foliar fertilizer by combining intertillage weeding when the new buds grow to 2cm and 2 true leaves grow, wherein the foliar fertilizer consists of the following raw materials in parts by weight: 20 parts of aloe powder, 6 parts of brassinolide, 8 parts of bamboo vinegar, 5 parts of sugar alcohol chelated liquid fertilizer, 10 parts of urea and 120 parts of water; and (5) transplanting the seedlings in the field after the seedlings are 9cm high and roots are developed.
Example 4
The method for raising the seedlings of the kudzuvine roots provided by the embodiment comprises the following steps:
(1) and (3) strain amplification culture: mixing brown sugar and wheat bran according to a mass ratio of 1:11, inoculating 2mL of probiotics, adding water with the same volume as the probiotics, and naturally fermenting for 25 hours to obtain a strain for later use;
(2) preparing a supernatant fluid: mixing the powder of the kudzu, the pineapple and the cedar wood chips according to the mass ratio of 2:2:1, then mixing the obtained mixture with the strain obtained in the step (1) according to the mass ratio of 11:1, putting the mixture into a tank, sealing and fermenting for 95 days, and filtering the fermented product to obtain a supernatant for later use;
(3) preparing a chelating solution: a. firstly, dissolving sodium selenite in water to obtain 130g/L sodium selenite aqueous solution as mother solution for later use; b. mixing the mother liquor obtained in the step a with an amino acid solution with the mass concentration of 30%, wherein the volume ratio of the mother liquor to the amino acid solution is 1: 4; then carrying out oscillation chelation for 72 hours at the temperature of 45 ℃ to obtain a chelating solution for later use;
(4) preparing a bud promoting agent: mixing the supernatant obtained in the step (2) with the chelating liquid obtained in the step (3) according to the volume ratio of 2:1, and then adding sodium alkyl benzene sulfonate, wherein the adding amount of the sodium alkyl benzene sulfonate is 6g per liter of chelating liquid; stirring at 35 ℃, and chelating for 48 hours to obtain an amino acid chelating sprouting promoter;
(II) seedling raising stage
Firstly, tissue culture seedling treatment: cleaning the tissue culture seedlings, soaking the tissue culture seedlings in carbendazim for 11min, then transplanting the tissue culture seedlings into seedling raising cups, and placing the seedling raising cups in seedling raising sheds;
seedling management: spraying water to the transplanted tissue culture seedlings in the morning and at night every day, maintaining the temperature of a seedling raising shed to be not lower than 18 ℃ and the humidity to be not lower than 70%, and spraying a sterilizing agent once every 8 days, wherein the sterilizing agent is a carbendazim solution diluted by 300 times by adding water; on the 4 th day after transplanting, diluting the amino acid chelating germination promoter obtained in the step (I) with water according to the volume ratio of 1:1000, then spraying the diluted solution on the tissue culture seedlings, and when new buds grow to 4cm and 3 true leaves grow, spraying a foliar fertilizer by combining intertillage weeding, wherein the foliar fertilizer consists of the following raw materials in parts by weight: 25 parts of aloe powder, 12 parts of brassinolide, 16 parts of bamboo vinegar, 10 parts of sugar alcohol chelated liquid fertilizer, 20 parts of urea and 200 parts of water; and (5) transplanting the seedlings in the field after the seedlings are 11cm high and roots are developed.
Effect verification
In order to further illustrate the practical value of the kudzu seedling method, the applicant makes a comparative test:
a first group: the kudzu vine root seedling raising method in embodiment 1 of the invention;
second group: the kudzu vine root seedling raising method in embodiment 3 of the invention;
third group: a traditional kudzu root cutting seedling method;
and a fourth group: a traditional kudzu root tissue culture seedling method.
S1: observing the conditions of cultivating the kudzuvine roots by the four groups of seedling cultivating methods, and recording the seedling cultivating conditions as shown in table 1:
TABLE 1 seedling growing situation comparison table
| Group of | Rooting percentage (%) | Percentage of Multi root (%) | Survival rate (%) | Time of seedling (Tian) |
| First group | 95 | 56 | 96 | 92 |
| Second group | 96 | 54 | 95 | 90 |
| Third group | 80 | 29 | 73 | 113 |
| Fourth group | 82 | 27 | 75 | 110 |
S2: observing the transplanted field, wherein the four groups of transplanting processes and the management after transplanting are the same, and observing the growth condition of the kudzu root seedlings after transplanting two months in the field. The survival rate of transplantation was counted and the results were recorded as shown in table 2:
TABLE 2 growth situation table for four groups of transplanted seedlings of radix Puerariae
The two tables show that: the kudzu roots cultivated by the cultivation method are healthy and strong in seedling bodies and strong in resistance, high-quality seedlings are obtained in the seedling cultivation stage, good growth can be kept after the seedlings are transplanted to a field, the steps are closely combined and interact, the survival rate of the kudzu roots is improved, and the cultivation method is suitable for large-scale planting and worthy of popularization.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (5)
1. A method for culturing seedlings of radix puerariae is characterized by comprising the following steps:
first, preparing bud promoting agent
(1) And (3) strain amplification culture: mixing brown sugar and wheat bran according to a mass ratio of 1: mixing according to the proportion of 10-11, inoculating 1-2mL of probiotics, adding water with the same volume as the probiotics, and naturally fermenting for 24-25 hours to obtain a strain for later use;
(2) preparing a supernatant fluid: mixing the powder of the kudzu, the pineapple and the cedar wood chips according to the mass ratio of 1-2:2:1, then mixing the obtained mixture with the strain obtained in the step (1) according to the mass ratio of 10-11:1, putting the mixture into a tank, sealing and fermenting for 90-95 days, and filtering the fermented product to obtain a supernatant for later use;
(3) preparing a chelating solution: a. firstly, dissolving sodium selenite in water to obtain 130g/L sodium selenite aqueous solution with the concentration of 120-; b. mixing the mother liquor obtained in the step a with an amino acid solution with the mass concentration of 20-30%, and then carrying out oscillation chelation for 48-72 hours at 35-45 ℃ to obtain a chelating solution for later use;
(4) preparing a bud promoting agent: mixing the supernatant obtained in the step (2) with the chelating liquid obtained in the step (3) according to the volume ratio of 3-4:1-2, and then adding sodium alkyl benzene sulfonate; stirring at 25-35 deg.C, chelating for 36-48 hr to obtain amino acid chelating germination promoter;
(II) seedling raising stage
1) Obtaining a cultured seed: cutting radix Puerariae into short vines with bud points and length of 8-9cm, wherein the upper part of the bud point is 1-2cm long, and the lower part is 7-8cm long, so as to avoid head and tail inversion and influence on growth;
2) pretreatment of a seedling raising field: ridging the field, wherein the ridge height is 15-20cm, and spraying carbendazim for disinfection;
3) cutting and seedling raising: diluting the amino acid chelating germination promoter obtained in the step (one) with water according to the volume ratio of 1:600-650, then soaking the brachypodium obtained in the step 1) in the diluted solution for 10-12min, taking out the brachypodium, vertically inserting the brachypodium into ridges, keeping the bud points on the soil, and completely immersing the lower parts of the bud points in the soil;
4) seedling management: after the bud point starts to sprout in 6-9 days after cuttage, spraying a solution prepared by mixing the amino acid chelating germination promoter obtained in the step (I) and water according to the proportion of 1: 1000-; when the new buds grow to 3-4cm and 2-3 true leaves grow, spraying a foliar fertilizer by combining intertillage weeding, and transplanting in a field when the height of seedlings is 9-11cm and the roots are developed;
the foliar fertilizer is prepared from the following raw materials in parts by weight: 20-25 parts of aloe powder, 6-12 parts of brassinolide, 8-16 parts of bamboo vinegar, 5-10 parts of sugar alcohol chelated liquid fertilizer, 10-20 parts of urea and 200 parts of 120-one water.
2. A method for raising seedlings of radix puerariae according to claim 1, wherein the seedling raising stage in the second step is replaced by the following steps:
3. A radix puerariae seedling raising method according to claim 1, wherein the volume ratio of the mother liquor to the amino acid solution in the step (3) is 1: 3-4.
4. A method for raising seedlings of radix puerariae according to claim 1, wherein 5-6g of sodium alkyl benzene sulfonate per liter of chelating solution is added in the step (4).
5. A method for raising seedlings of radix puerariae according to claim 2, wherein the step is carried out
The bactericidal medicine in the (1) is carbendazim solution diluted by 300 times by adding water.
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