CN109456091A - Utilize the method and application of cowboying wastewater fermentation production microbial manure - Google Patents
Utilize the method and application of cowboying wastewater fermentation production microbial manure Download PDFInfo
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- CN109456091A CN109456091A CN201811032861.2A CN201811032861A CN109456091A CN 109456091 A CN109456091 A CN 109456091A CN 201811032861 A CN201811032861 A CN 201811032861A CN 109456091 A CN109456091 A CN 109456091A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a kind of methods and application using cowboying wastewater fermentation production microbial manure, wherein, the supplementary carbon source into cowboying waste water, obtain pretreated waste water, the inoculated plant plant growth-promoting rhizobacteria into pretreated waste water, fermented and cultured to get arrive the microbial manure, wherein, sterilization treatment is done to cowboying waste water or pretreated waste water.The present invention passes through using cowboying waste water as culture substrate, make full use of various nutriments in waste water, direct microbial fermentation is carried out after supplementing certain carbon source, the waste water for handling discharge by sky high cost is needed to become multifunctional microbial fertilizer tradition, turn waste into wealth, the utilization rate to breeding wastewater is improved, reduces the production cost of production microbial manure, and there is effect outstanding to crop-plantings such as capsicum, tomato, eggplants.
Description
Technical field
The present invention relates to breeding wastewater technical field of resource utilization, it is more particularly related to which a kind of utilize cowboying
The method and application of wastewater fermentation production microbial manure.
Background technique
Official, agriculture rural area portion data show that, currently, national about 3,800,000,000 tons of yield of livestock and poultry feces year, wherein livestock and poultry are direct
About 1,800,000,000 tons of the excrement of excretion, generate about 2,000,000,000 tons of sewage quantity of breeding process, rate is effectively treated not in generally breeding wastewater
To 50%, the resource utilization space of breeding wastewater is very big.
Currently, China's cultivating wastewater purification mainly uses Anaerobic Treatment (biogas fermentation), Aerobic Process for Treatment and anaerobism-good
The different disposals combined systems such as oxygen processing.Anaerobic methane fermentation materials conversion ratio is low, unstable, and biogas slurry secondary pollution is serious, drop
Low soil quality, American-European majority state have disabled.Aerobic Process for Treatment and anaerobic-aerobic processing are purely acting as breeding wastewater
It is discharged after offal treatment, input cost is high, and without any economy return, breeding enterprise and raiser are reluctant to use.
On the other hand, application of the microbial manure in agricultural increasingly obtains the attention of people, and microbial manure, which has, to be increased
It produces, is disease-resistant, improving crop quality, the ahead of time multiple efficacies such as crop maturity, organic fertilizer or microbial manure are applied into soil,
Beneficial microbe group in soil can also gradually be restored, eliminate soil hardening etc..Promoting the use of microbial manure is to realize to subtract
The important channel of few chemical fertilizer and pesticide dosage target, this theory have been increasingly becoming people's common recognition.
Data shows cowboying waste water nitrogen rich in, phosphorus, and containing nutritional ingredients such as a certain amount of total reducing sugar and potassium, and
These are that breeding wastewater is used to prepare microbial manure to its resource utilization with important necessary to microorganism growth
Value, still, there is presently no simple and direct, the efficient methods that breeding wastewater is used to prepare to microbial manure.
Summary of the invention
It is an object of the invention to solve at least the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of methods using cowboying wastewater fermentation production microbial manure, can
Using cowboying give up aerobic fermentation production microbial manure.
It is a still further object of the present invention to provide it is a kind of using cowboying wastewater fermentation production microbial manure capsicum, kind
Application in eggplant plantation, can promote plant growth.
In order to realize these purposes and other advantages according to the present invention, a kind of utilization cowboying wastewater fermentation production is provided
The method of microbial manure, wherein supplementary carbon source in Xiang Yangniu waste water obtains pretreated waste water, is inoculated with into pretreated waste water
Plant growth-promoting rhizobacteria, fermented and cultured to get arrive the microbial manure, wherein cowboying waste water or pretreated waste water are done and gone out
Bacterium processing.
Preferably, the microbial manure is liquid microbial fertilizer.
Preferably, the Biocidal treatment method is 80-100 DEG C of heating 5-10min.
Preferably, the carbon source supplemented is one or more of glucose, starch, sucrose, molasses, sodium acetate, carbon
Source magnitude of recruitment using carbon-nitrogen mass ratio in cowboying waste water be 1:3-5:1 as standard.
Preferably, the plant growth-promoting rhizobacteria is bacillus, specially bacillus subtilis, huge gemma bar
Bacterium, bacillus licheniformis, bacillus amyloliquefaciens, Methylotrophic bacillus, Te Jila bacillus and Bei Laisi bud
One or more of spore bacillus.
Preferably, the plant growth-promoting rhizobacteria is the antimicrobial composition of the bacterial strain mixing composition of following bacillus
Object: bacillus subtilis ACCC19743, bacillus amyloliquefaciens ACCC10167, Methylotrophic bacillus ACCC19730
And Bei Laisi bacillus ACCC 03040.
Preferably, when inoculation bacterium is the microbial composite, carbon source magnitude of recruitment is with carbon-nitrogen mass ratio in cowboying waste water
It is standard for 4.2:1.
Preferably, the specific method of the microbial composite is inoculated with into pretreated waste water the following steps are included:
Step 1, actication of culture, the bacterial strain in microbial composite are inoculated with LB, PCA or NA slant medium respectively, and 30 ± 2
DEG C incubator activation culture 24-30h, the strain after being activated；
Strain after activation is inoculated with liquid seed culture medium, 200 ± 50r/min at a temperature of 30 ± 2 DEG C by step 2 respectively
Shaken cultivation 20-30h, obtains liquid seeds, wherein the liquid seed culture medium comprises the following components in parts by weight: starch
13-18 parts, 18-22 parts of dregs of beans, 0.4-0.6 parts of potassium dihydrogen phosphate, 0.2 part of bitter salt, 0.8-1.2 parts of sodium chloride, carbon
Sour calcium 0.4-0.6 parts, 1000 parts of water adjust pH7.0,121 DEG C of sterilizing 20min；
The liquid seeds equal proportion of each bacterial strain of the microbial composite is inoculated in by sterilization treatment by step 3
Pretreated waste water carry out fermented and cultured, total inoculum concentration be pretreated waste water total volume 0.5-5.0%, fermentation condition 25-
35 DEG C, ventilatory capacity 0.3-1.0vvm, 150-400rpm is stirred, fermentation time 24-36h, fermentation ends are up to the microorganism fertilizer
Material.
A kind of application using cowboying wastewater fermentation production microbial manure in pepper planting, wherein application method are as follows:
It waters, the liquid microbial fertilizer is applied with water punching, amount of application is 10-20kg liquid microbe fertilizer per acre by after.
A kind of application using cowboying wastewater fermentation production microbial manure in eggplant plantation, wherein application method are as follows:
It waters, the liquid microbial fertilizer is applied with water punching, amount of application is that 10-20kg liquid microbe is fertile per acre after eggplant is transplanted
Material.
The present invention is include at least the following beneficial effects:
Firstly, the present invention is by using cowboying waste water as culture substrate, making full use of various nutriments in waste water, supplement one
After fixed carbon source, by plant growth-promoting rhizobacteria fermenting and producing microbial manure, tradition is needed to handle by sky high cost and is arranged
The waste water put becomes multifunctional microbial fertilizer, turns waste into wealth, and improves the utilization rate to breeding wastewater.
Secondly, changing traditional microbiological fertilizer production mode through the invention, traditional microbiological fertilizer production needs disappear
The nitrogen sources such as the carbon sources such as a large amount of starch, glucose and dregs of beans are consumed, and the present invention is only needed using cowboying waste water as primary raw material, supplement
A small amount of carbon source, has saved grain resource, and the production cost of production microbial manure is greatly lowered.
Finally, present invention gained microbial manure has effect outstanding in capsicum, tomato planting.Pass through Field information
Experiment shows 10% or more bud ratio when the microbial manure can significantly improve capsicum, tomato seedling, shorten capsicum,
The growing-seedling period of the vegetables such as tomato at least 3-4 days；Promote eggplant to budding in advance 10-15 days, lists 15 days or so ahead of time, and improve
Disease resistance ability, hence it is evident that promote plant growth.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
The present invention provides a kind of method using cowboying wastewater fermentation production microbial manure, wherein in Xiang Yangniu waste water
Supplementary carbon source obtains pretreated waste water, the inoculated plant plant growth-promoting rhizobacteria into pretreated waste water, and fermented and cultured is to get described in
Microbial manure, wherein sterilization treatment is done to cowboying waste water or pretreated waste water.
In the program, by using cowboying waste water as culture substrate, making full use of various nutriments in waste water, supplement is certain
Carbon source after, by plant growth-promoting rhizobacteria fermenting and producing microbial manure, tradition is needed to handle by sky high cost and is discharged
Waste water become multifunctional microbial fertilizer, turn waste into wealth, improve the utilization rate to breeding wastewater.In addition, the present invention changes
Traditional microbiological fertilizer production mode, traditional microbiological fertilizer production need to consume the carbon sources such as a large amount of starch, glucose and
The nitrogen sources such as dregs of beans, and the present invention only needs to supplement a small amount of carbon source using cowboying waste water as primary raw material, has saved grain money
The production cost of production microbial manure is greatly lowered in source, and gained microbial manure has in capsicum, tomato planting
Effect outstanding.
In one preferred embodiment, the microbial manure is liquid microbial fertilizer.
In above scheme, by liquid state fermentation product directly as liquid biological Fertilizer application, simplify production technology, application side
Just.
In one preferred embodiment, the Biocidal treatment method is 80-100 DEG C of heating 5-10min.
In above scheme, cowboying waste water is pre-processed by the sterilization method, the disease in cowboying waste water can be killed
Pathogenic microorganism and roundworm egg, killing rate reach 99.9% or more, ensure the safety using cowboying waste water production microbial manure
Property.
In one preferred embodiment, the carbon source supplemented is one of glucose, starch, sucrose, molasses, sodium acetate or several
Kind, carbon source magnitude of recruitment using carbon-nitrogen mass ratio in cowboying waste water be 1:3-5:1 as standard.
In above scheme, although higher by detection cowboying waste water nitrogen content, phosphorus content is insufficient, by suitably supplementing
Within the scope of carbon source makes the carbon-nitrogen ratio in cowboying waste water reach suitable fermenting plant rhizosphere microorganism, fermentation efficiency is improved.
In one preferred embodiment, the plant growth-promoting rhizobacteria is bacillus, specially bacillus subtilis, huge bud
Spore bacillus, bacillus licheniformis, bacillus amyloliquefaciens, Methylotrophic bacillus, Te Jila bacillus and Bei Lai
One or more of this bacillus.Bacterial strain uses therefor can be China General Microbiological Culture Collection Center, the micro- life of Chinese agriculture
Multiple bacterial strains that the open approach such as object Culture Collection Center is commercially available.
In one preferred embodiment, the plant growth-promoting rhizobacteria is micro- life that the bacterial strain mixing of following bacillus is constituted
Compositions: bacillus subtilis ACCC19743, bacillus amyloliquefaciens ACCC10167, Methylotrophic bacillus
ACCC19730 and Bei Laisi bacillus ACCC 03040.The bacterial strain group, which is combined into, produces microbial manure using cowboying waste water
Optimum strain combination.
In one preferred embodiment, when inoculation bacterium is the microbial composite, carbon source magnitude of recruitment is with carbon nitrogen in cowboying waste water
Mass ratio is that 4.2:1 is standard.The ferment effect of antimicrobial composition is best under the ratio.
In one preferred embodiment, the specific method that the microbial composite is inoculated with into pretreated waste water includes following step
It is rapid:
Step 1, actication of culture, the bacterial strain in microbial composite are inoculated with LB, PCA or NA slant medium respectively, and 30 ± 2
DEG C incubator activation culture 24-30h, the strain after being activated.
Strain after activation is inoculated with liquid seed culture medium, 200 ± 50r/min at a temperature of 30 ± 2 DEG C by step 2 respectively
Shaken cultivation 20-30h, obtains liquid seeds, wherein the liquid seed culture medium comprises the following components in parts by weight: starch
13-18 parts, 18-22 parts of dregs of beans, 0.4-0.6 parts of potassium dihydrogen phosphate, 0.2 part of bitter salt, 0.8-1.2 parts of sodium chloride, carbon
Sour calcium 0.4-0.6 parts, 1000 parts of water adjust pH7.0,121 DEG C of sterilizing 20min.
The liquid seeds equal proportion of each bacterial strain of the microbial composite is inoculated in by sterilization treatment by step 3
Pretreated waste water carry out fermented and cultured, total inoculum concentration be pretreated waste water total volume 0.5-5.0%, fermentation condition 25-
35 DEG C, ventilatory capacity 0.3-1.0vvm, 150-400rpm is stirred, fermentation time 24-36h, fermentation ends are up to the microorganism fertilizer
Material.
A kind of application using cowboying wastewater fermentation production microbial manure in pepper planting, wherein application method are as follows:
Capsicum is after planting watered, the liquid microbial fertilizer is applied with water punching, amount of application is that 10-20kg liquid microbe is fertile per acre
Material.
A kind of application using cowboying wastewater fermentation production microbial manure in tomato planting, wherein application method are as follows:
Tomato is after planting watered, and the liquid microbial fertilizer is applied with water punching, amount of application is that 10-20kg liquid microbe is fertile per acre
Material.
A kind of application using cowboying wastewater fermentation production microbial manure in eggplant plantation, wherein application method are as follows:
It waters, the liquid microbial fertilizer is applied with water punching, amount of application is that 10-20kg liquid microbe is fertile per acre after eggplant is transplanted
Material.
Embodiment 1
The Preliminary detection of cowboying waste water: Hebei Shijiazhuang Xinji vaccary waste water is taken, using rapid-digestion spectrophotometric
Method (HJ/T 399-2007, the measurement rapid-digestion spectrophotometry of water chemical oxygen demand) measures CODCrFor 9565 ± 35mg/
L, using distillation-neutralization titration (measurement of HJ 537-2009, Water quality ammonia nitrogen use distillation-neutralization titration) measurement ammonia nitrogen
For 363 ± 9.5mg/L, ultraviolet spectrophotometry (GB 11894-89, the measurement of water quality total nitrogen are cleared up using alkaline chitinase
Alkaline chitinase is used to clear up ultraviolet spectrophotometry) measurement total nitrogen content is 664 ± 11.3mg/L.It is useless that DNS method measures this
211 ± 7.3mg/L of content of reducing sugar in water, 382 ± 9.2mg/L of total sugar content.The result shows that carbon source content is inclined in cowboying waste water
It is low, and nitrogen source content is higher.
Pretreatment: collecting above-mentioned cowboying waste water, and the fresh cowboying waste water of 6L is packed into 10L fermentor, and 15g grape is added
Sugar, 90 DEG C of insulated sterilizing 10min, is cooled to 30 DEG C, is pretreated waste water.
Actication of culture: bacillus subtilis ACCC19743, bacillus amyloliquefaciens ACCC10167, Methylotrophic gemma
Bacillus ACCC19730 and Bei Laisi bacillus ACCC 03040 is inoculated with PCA slant medium, 30 DEG C of incubator activation cultures
For 24 hours, strain after being activated.PCA inclined-plane culture based formulas is following (every liter): tryptone 5g, yeast extract powder 2.5g, glucose
1g, agar 15g, final pH 7.0 ± 0.2.
Liquid spawn preparation: strain after activation is taken to be inoculated with following seed culture mediums: starch 15g, dregs of beans 20g, phosphoric acid respectively
Potassium dihydrogen 0.5g, bitter salt 0.2g, sodium chloride 1g, calcium carbonate 0.5g, water 1L, pH7.0 dispense 100mL/500mL tri-
Angle bottle, 121 DEG C of sterilizing 20min.2 ring of inclined-plane seed of every bottle of fresh activation of inoculation, sets 30 DEG C of shaking table, 200r/min shaken cultivation
20h obtains liquid spawn.
Fermented and cultured: being seeded to pretreated waste water for each 50mL of liquid seeds of 4 plants of bacterium of gained, 30 DEG C, 250rpm stirring,
Ventilatory capacity 200L/h ferments 30h to get liquid microbial fertilizer is arrived.
Liquid microbe microbial inoculum dilution spread obtained by above-mentioned fermentation is taken to count after PCA plate, 30 DEG C of culture 48h, total bacterium
Number observes spore forming rate 94%, miscellaneous bacteria rate < 1% up to 6.58 hundred million cfu/mL, spore staining.
Embodiment 2
By bacillus subtilis ACCC19743, the bacillus amyloliquefaciens of the fresh activation of PCA slant medium
ACCC10167, Methylotrophic bacillus ACCC19730 are inoculated with following seed culture mediums: starch 20g, dregs of beans
40g, potassium dihydrogen phosphate 0.5g, bitter salt 0.2g, sodium chloride 1g, calcium carbonate 0.1g, water 1L, pH7.0 dispense 100mL/
500mL triangular flask, 121 DEG C of sterilizing 20min.Set 30 DEG C of shaking table, 200r/min shaken cultivation 20h obtains liquid seeds.
The total 1000mL of each 200mL of liquid seeds of 3 plants of bacterium of gained is inoculated in 600L sodium acetate containing 4.5g/L, 85 DEG C of sterilizings
8min, and naturally cool in 30 DEG C of pretreated waste water, 28 DEG C, 200rpm stirring, ventilatory capacity 250L/min, ferment 30h, obtains
To liquid microbe microbial inoculum, that is, liquid microbial fertilizer.
Aforesaid liquid microbial inoculum is taken to carry out spore staining observation spore forming rate>95%, miscellaneous bacteria rate<1% with peacock green sarranine；
Dilution spread counts after PCA plate, 30 DEG C of culture 48h, and total bacteria count is up to 5.78 hundred million cfu/mL.
Embodiment 3
The experiment of capsicum field seedling raising
Dosage: experimental group 15kg liquid microbe fertilizer per acre, (nitrogen, phosphorus, potassium contain 50kg ternary compound fertilizer control group per acre
Amount is that 15%), other conditions are consistent.
Usage: after planting watering immediately, and Sui Shuichong is applied.
Experimental result: experimental group improves 10% relative to the bud ratio of control group, and nursery stage reduces 4 days, and experimental group is not applied
With any pesticide, plant strain growth stalwartness.
Embodiment 4
The experiment of tomato field seedling raising
Dosage: the first experimental group 15kg liquid microbe fertilizer per acre；Control group applies 50kg ternary compound fertilizer per acre
(nitrogen, phosphorus, potassium content are 15%).
Usage: after planting watering immediately, and Sui Shuichong is applied.
Experimental result: experimental group improves 9% relative to the bud ratio of control group, and growing-seedling period shortens 3-4 days, buddings in advance
10-15 days, listing 15 days or so is improved, experimental group does not apply any pesticide, and plant strain growth is healthy and strong.
Embodiment 5
The experiment of eggplant field-transplanting
Dosage: the first experimental group 15kg liquid microbe fertilizer per acre；Control group applies 50kg ternary compound fertilizer per acre
(nitrogen, phosphorus, potassium content are 15%).
Usage: watering immediately after transplanting, and Sui Shuichong is applied.
Experimental result: the microbial manure has a significant effect to the transplanting of eggplant, and experimental group is relative to control group
Budding in advance 10-15 days, improve listing 15 days or so, experimental group does not apply any pesticide, and plant strain growth is healthy and strong.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and shown here as with embodiment.
Claims (10)
1. a kind of method using cowboying wastewater fermentation production microbial manure, wherein supplementary carbon source in Xiang Yangniu waste water obtains
Pretreated waste water, the inoculated plant plant growth-promoting rhizobacteria into pretreated waste water, fermented and cultured to get arrive the microbial manure,
In, sterilization treatment is done to cowboying waste water or pretreated waste water.
2. utilizing the method for cowboying wastewater fermentation production microbial manure as described in claim 1, wherein the microorganism fertilizer
Material is liquid microbial fertilizer.
3. utilizing the method for cowboying wastewater fermentation production microbial manure as described in claim 1, wherein the sterilization treatment
Method is 80-100 DEG C of heating 5-10min.
4. utilizing the method for cowboying wastewater fermentation production microbial manure as described in claim 1, wherein the carbon source supplemented
For one or more of glucose, starch, sucrose, molasses, sodium acetate, carbon source magnitude of recruitment is with carbon-nitrogen mass ratio in cowboying waste water
It is standard for 1:3-5:1.
5. utilizing the method for cowboying wastewater fermentation production microbial manure as claimed in claim 1 or 2, wherein the plant
Plant growth-promoting rhizobacteria is bacillus, specially bacillus subtilis, bacillus megaterium, bacillus licheniformis, solution starch gemma
One or more of bacillus, Methylotrophic bacillus, Te Jila bacillus and Bei Laisi bacillus.
6. utilizing the method for cowboying wastewater fermentation production microbial manure as claimed in claim 5, wherein the plant rhizosphere
Promoting bacteria is the microbial composite that the bacterial strain mixing of following bacillus is constituted: bacillus subtilis ACCC19743, Xie Dian
Afnyloliquefaciens ACCC10167, Methylotrophic bacillus ACCC19730 and Bei Laisi bacillus ACCC 03040.
7. utilizing the method for cowboying wastewater fermentation production microbial manure as claimed in claim 6, wherein inoculation bacterium is described
When microbial composite, carbon source magnitude of recruitment is 4.2:1 as standard using carbon-nitrogen mass ratio in cowboying waste water.
8. utilizing the method for cowboying wastewater fermentation production microbial manure as claimed in claim 6, wherein to pretreated waste water
The specific method of the middle inoculation microbial composite the following steps are included:
Step 1, actication of culture, the bacterial strain in microbial composite are inoculated with LB, PCA or NA slant medium, 30 ± 2 DEG C of trainings respectively
Support case activation culture 24-30h, the strain after being activated；
Strain after activation is inoculated with liquid seed culture medium by step 2 respectively, and 200 ± 50r/min vibrates at a temperature of 30 ± 2 DEG C
20-30h is cultivated, obtains liquid seeds, wherein the liquid seed culture medium comprises the following components in parts by weight: starch 13-18
Part, 18-22 parts of dregs of beans, 0.4-0.6 parts of potassium dihydrogen phosphate, 0.2 part of bitter salt, 0.8-1.2 parts of sodium chloride, calcium carbonate
0.4-0.6 parts, 1000 parts of water adjust pH7.0,121 DEG C of sterilizing 20min；
The liquid seeds equal proportion of each bacterial strain of the microbial composite is inoculated in by the pre- of sterilization treatment by step 3
It handling waste water and carries out fermented and cultured, total inoculum concentration is the 0.5-5.0% of pretreated waste water total volume, and fermentation condition is 25-35 DEG C,
Ventilatory capacity 0.3-1.0vvm stirs 150-400rpm, and fermentation time 24-36h, fermentation ends are up to the microbial manure.
9. a kind of application using cowboying wastewater fermentation production microbial manure in pepper planting as claimed in claim 6,
Wherein, application method are as follows: capsicum is after planting watered, the liquid microbial fertilizer is applied with water punching, amount of application is 10- per acre
20kg liquid microbe fertilizer.
10. a kind of application using cowboying wastewater fermentation production microbial manure in eggplant plantation as claimed in claim 6,
Wherein, application method are as follows: water, the liquid microbial fertilizer is applied with water punching, amount of application is 10- per acre after transplanting eggplant
20kg liquid microbe fertilizer.
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| CN112479391A (en) * | 2020-11-04 | 2021-03-12 | 中国石油天然气集团有限公司 | Preparation method of degradation product of well site environment-friendly toilet and method for treating water-based solid waste |
| CN115353986A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Bacillus belgii strain WB strain for treating swine wastewater and application thereof |
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| CN115353986B (en) * | 2022-01-28 | 2023-07-07 | 齐齐哈尔大学 | Bacillus bailii strain WB strain for treating pig raising wastewater and application thereof |
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