CN110066194A - Novel more bacterium composite microbe fertilizers of one kind and its preparation method and application - Google Patents

Novel more bacterium composite microbe fertilizers of one kind and its preparation method and application Download PDF

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Publication number
CN110066194A
CN110066194A CN201910262313.7A CN201910262313A CN110066194A CN 110066194 A CN110066194 A CN 110066194A CN 201910262313 A CN201910262313 A CN 201910262313A CN 110066194 A CN110066194 A CN 110066194A
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novel
composite microbe
fermentation
bacterium
bacterium composite
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Inventor
朱春苗
刘玉珍
黄娟
郭小红
李肖宇
翟修彩
揭云东
钱潘攀
余劲聪
骆毛喜
林克明
陈晓燕
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Zhangzhou Sunju Biology Technology Co ltd
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Zhangzhou Sunju Biology Technology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a kind of novel more bacterium composite microbe fertilizers and preparation method thereof and its application, are related to bacterial manure preparation technical field.Novel more bacterium composite microbe fertilizers of the present invention after inoculation bacillus laterosporus fermentation, form primary fermentation material using pig manure, seaweed, mushroom slag and stalk as initial raw material;Bacillusmusilaginosiengineering fermentation liquid is inoculated into the primary fermentation material, then is fermented a period of time, novel more bacterium composite microbe fertilizers are finally obtained.Crop rhizosphere can be promoted to develop using novel more bacterium composite microbe fertilizers that preparation method of the present invention is prepared, both nutrition in all directions was provided for crop, crop disease-resistant ability can also be improved, there is good inhibiting effect to the pathogen of crop, have certain upgrading effect of increasing production to crop.

Description

Novel more bacterium composite microbe fertilizers of one kind and its preparation method and application
Technical field
The invention belongs to bacterial manure preparation technical fields, and in particular to the novel more bacterium composite microbe fertilizers of one kind and its preparation Method and and application.
Background technique
With the raising of the level of urbanization, the demand of meat eggs is continuously improved in people, and livestock and poultry cultivation number increases Add, popularization.Intensive pig production brings a large amount of pig manure, and the untreated serious polluted agricultural land ecological environment of pig manure causes Surface water body eutrophication brings stench etc..Domestic excrement and urine treatment technology mainly has compost fermentation method, biogas fermentation method etc..
About the processing of pig manure, there is no particularly preferred schemes at present, utilize pig as CN201510769449.9 is disclosed The method of excrement and mushroom residue growth biological organic fertilizer, it is rotten that this method has selected bacillus subtilis to carry out pig manure and mushroom residue It is ripe, then the probiotics with antagonism or inhibiting effect harmful fungoid is compounded, the survival rate of this method probiotics is lower, in order to protect A large amount of probiotics must be used by demonstrate,proving survival rate, increase cost;For another example CN201410514541.6 discloses a kind of using micro- Biological fermentation process prepares the technique of pig manure bio-organic fertilizer, and this method is added to 10%-20% seaweed in compost ingredient, makes The biological organic fertilizer that must be prepared has the advantages that alga fertilizer, but preparation process is complicated, inconvenient agricultural production practical operation;Also such as CN201010228347.3, CN201310666396.9, CN20130308892.7 are each provided with the stalk for compost fermentation Decomposing microbial inoculum, and stalk compost fermentation have the shortcomings that it is labor intensive, at high cost.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of novel more bacterium composite microbe fertilizers and preparation method thereof and Using crop rhizosphere capable of being promoted to develop, both provide nutrition in all directions for crop, moreover it is possible to improved crop disease-resistant ability, have to crop Certain upgrading effect of increasing production.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of novel more bacterium composite microbe fertilizers, the preparations of novel more bacterium composite microbe fertilizers Method the following steps are included: (1) by pig manure, seaweed, mushroom slag and stalk according to (35~45): (15~25): (25~35): (5 ~15) mass ratio mixing carries out the first fermentation, obtains the first fermentation material;
(2) microbial inoculum is inoculated into first fermentation material, is uniformly mixed and carries out the second fermentation, obtains novel more bacterium Composite microbe fertilizer;The microbial inoculum includes bacillus laterosporus and bacillusmusilaginosiengineering.
Preferably, the mass ratio of step (1) pig manure, seaweed, mushroom slag and stalk is (36~43): (16~24): (26~34): (8~13).
Preferably, to stack fermentation, the time of first fermentation is 12~18d for step (1) first fermentation.
Preferably, step (2) bacillus laterosporus and bacillusmusilaginosiengineering individually carry out the inoculation.
Preferably, step (2) described bacillusmusilaginosiengineering includes bacillusmusilaginosiengineering No. 01 bacterial strain of three torches, preservation Number is CGMCC NO.3995.
Preferably, the inoculation volume of the bacillus laterosporus is the 10~30% of the first fermentation material volume.
Preferably, the inoculation volume of the bacillusmusilaginosiengineering is the first hair after the inoculation bacillus laterosporus The 10~15% of ferment object product.
Preferably, water content when step (2) described second is fermented in system is 20~45%.
The present invention also provides novel more bacterium composite microbe fertilizers to improve the application in crop yield and quality.
Preferably, the crop includes spinach, capsicum and tomato.
The present invention provides a kind of novel more bacterium composite microbe fertilizers, using pig manure, seaweed, mushroom slag and stalk as initially Raw material is for the first fermentation, continues to ferment after being inoculated with bacillus laterosporus and bacillusmusilaginosiengineering, finally obtains institute State novel more bacterium composite microbe fertilizers.Novel more bacterium composite microbe fertilizers that the present invention is prepared can promote root of the crop Border development, both provided nutrition in all directions for crop, moreover it is possible to improve crop disease-resistant ability, have certain upgrading effect of increasing production to crop.
Biological deposits explanation
Bacillusmusilaginosiengineering (Bacillus mucilaginosus), preservation place are Chinese microorganism strain preservation Administration committee's common micro-organisms center, specific address are that the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences is micro- Biological study institute, preservation time are on July 2nd, 2010, and deposit number is CGMCC NO.3995.
Specific embodiment
The present invention provides a kind of novel more bacterium composite microbe fertilizers, the preparations of novel more bacterium composite microbe fertilizers Method the following steps are included: (1) by pig manure, seaweed, mushroom slag and stalk according to (35~45): (15~25): (25~35): (5 ~15) mass ratio mixing carries out the first fermentation, obtains the first fermentation material;
(2) microbial inoculum is inoculated into first fermentation material, is uniformly mixed and carries out the second fermentation, obtains novel more bacterium Composite microbe fertilizer;The microbial inoculum includes bacillus laterosporus and bacillusmusilaginosiengineering.
In the preparation method of novel more bacterium composite microbe fertilizers of the present invention, by pig manure, seaweed, mushroom slag and stalk According to (35~45): (15~25): (25~35): the mass ratio mixing of (5~15) carries out the first fermentation, obtains the first fermentation material. Pig manure of the present invention, seaweed, mushroom slag and stalk mass ratio be preferably (36~43): (16~24): (26~34): (8~ 13), more preferably (38~41): (19~22): (27~32): (9~12), most preferably 40:20:30:10.The present invention couple There is no particular determinations for the mixing, are preferably mixed in fermentation vat.The present invention to the pig manure, seaweed, mushroom slag and There is no particular determinations in the source of stalk, utilize the conventional commercial product of this field.The present invention is to the mixed original Material carry out first fermentation, it is described first fermentation preferably stack fermentation, it is described first fermentation material moisture be 50~ 60%, more preferably 52~56%, most preferably 55%.Fermentation ends water content is controlled 20~40%.First fermentation Time be preferably 12~18d, more preferably 14~16d, most preferably 15d.The present invention to it is described stack fermentation method simultaneously It is not particularly limited, conventional using this field stacks fermentation process.
After obtaining the first fermentation material, microbial inoculum is inoculated into first fermentation material by the present invention, is uniformly mixed and is carried out the second hair Ferment obtains novel more bacterium composite microbe fertilizers;The microbial inoculum includes bacillus laterosporus and bacillusmusilaginosiengineering.This hair The bright microbial inoculum be preferably it is fermented after bacterium solution.The concentration of viable bacteria is preferably 2~1,000,000,000/mL in the bacterium solution, more preferably 4~900,000,000/mL, most preferably 8.5 hundred million/mL.
Microbial inoculum of the present invention is preferably independent inoculation, is inoculated with bacillus laterosporus first in the present invention, inoculates glue Freeze sample bacillus.The inoculation volume of bacillus laterosporus of the present invention be preferably the first fermentation material volume 10~ 30%, more preferably 15~25%, most preferably 20%.The present invention does not have special limit to the source of the bacillus laterosporus It is fixed, utilize the conventional commercial strain of this field.
The bacillus laterosporus is inoculated in after the first fermentation material preferably after mixing by the present invention, is inoculated described Bacillusmusilaginosiengineering.The inoculation volume of bacillusmusilaginosiengineering of the present invention is preferably the inoculation bacillus laterosporus The 10~15% of the first fermentation material volume afterwards, more preferably 11~14%, most preferably 13%.Gel-shaped of the present invention Bacillus preferably includes bacillusmusilaginosiengineering No. 01 bacterial strain of three torches, and deposit number is CGMCC NO.3995.
When the present invention ferments to the second fermentation of system progress after the inoculation microbial inoculum, described second, preferably in control system Water content be 20~40%, more preferably 25%.In the described second fermentation, the heap temperature of such as fermentation reaches the present invention 55 DEG C or more, preferably further include carrying out turning over throwing.There is no particular determinations to the method for turning over throwing by the present invention, preferably with turning over Throwing machine carries out turning over throwing.The time of second fermentation of the present invention is preferably 15~20d, more preferably 16~18d, most preferably 17d。
In the present invention, pig manure, seaweed, mushroom slag and stalk proportionally mix, and ferment by first time, and zymophyte will Nutriment needed for the organic substance of the inside is converted into organism, the microorganism for second of fermentation are absorbed and utilized, add Fast reproduction speed generates more organisms.After fermenting by first time, more favorable fermentation item is provided for second of fermentation Part.After fermenting twice, obtained organic fertilizer odorless, granularity is less than 10mm, and carbon-nitrogen ratio is moderate (being less than 20:1), more preferably Can be absorbed by crops utilization, directly contact crop root use be not in burn seedlings phenomenon.
Compound bacteria in novel more bacterium composite microbe fertilizers of the present invention can generate a variety of metabolites, such as inhibit disease Protide (inhibiting phytophthora blight of pepper and Rhizoctonia solani Kuhn), the Bacillus amine, polyketides, lipopeptide compound of opportunistic pathogen Equal substances, composite thallus can also secrete the noxious materials such as polysaccharose substance degrading pesticide, also have phosphorus decomposing, potassium decomposing, fixed nitrogen Etc. multiple biological functions.Simultaneously bacillus laterosporus and bacillusmusilaginosiengineering can degrade nitrate content in soil, mention The content of high soil effective K, substantially increase the utilization rate of fertilizer, to play improvement soil and crop yield synergy Double action.
The present invention also provides novel more bacterium composite microbe fertilizers that the preparation method is prepared to make produce in raising Application in amount and quality.There is no particular determinations for type of the present invention to the crop, preferably include spinach, capsicum and sage Female fruit.The amount of application of novel more bacterium composite microbe fertilizers of the present invention is preferably 64kg/ mus.
Below with reference to embodiment more bacterium composite microbe fertilizers novel to one kind provided by the invention preparation method and its answer With being described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
1, raw material mixes: pig manure, seaweed, mushroom slag, stalk are ready to according to mass ratio 40:20:30:10, are deposited in fermentation Chi Zhong, and be uniformly mixed, stack fermentation 15 days;
2, the independent bacillus laterosporus fermentation liquid (5.5 hundred million/ml of viable count) got ready is sprinkled into 15% inoculum concentration In above-mentioned mixed raw material, it is uniformly mixed;
3, the independent bacillusmusilaginosiengineering fermentation liquid (300,000,000/ml of viable count) got ready is sprinkled into 15% inoculum concentration In above-mentioned mixed raw material, it is uniformly mixed;
3, water content 25% or so is controlled, is fermented.Moisture state is kept in fermentation process, water content is observed in timing Variation, is replenished in time moisture.Reach 55 DEG C or more to heap temperature, is carried out turning over throwing with turner, fermentation time is 15 days, i.e., Obtain novel more bacterium composite microbe fertilizers.
Embodiment 2
1, raw material mixes: pig manure, seaweed, mushroom slag, stalk are ready to according to mass ratio 40:20:30:10, are deposited in fermentation Chi Zhong, and be uniformly mixed, stack fermentation 15 days;
2, the independent bacillus laterosporus fermentation liquid (4.5 hundred million/ml of viable count) got ready is sprinkled into 20% inoculum concentration In above-mentioned mixed raw material, it is uniformly mixed;
3, the independent bacillusmusilaginosiengineering fermentation liquid (400,000,000/ml of viable count) got ready is sprinkled into 13% inoculum concentration In above-mentioned mixed raw material, it is uniformly mixed;
3, water content 25% or so is controlled, is fermented.Moisture state is kept in fermentation process, water content is observed in timing Variation, is replenished in time moisture.Reach 55 DEG C or more to heap temperature, is carried out turning over throwing with turner, fermentation time is 18 days, i.e., Obtain novel more bacterium composite microbe fertilizers.
Embodiment 3
1, raw material mixes: pig manure, seaweed, mushroom slag, stalk are ready to according to mass ratio 40:20:30:10, are deposited in fermentation Chi Zhong, and be uniformly mixed, stack fermentation 15 days;
2, the independent bacillus laterosporus fermentation liquid (400,000,000/ml of viable count) got ready is sprinkled into 25% inoculum concentration It states in mixed raw material, is uniformly mixed;
3, the independent bacillusmusilaginosiengineering fermentation liquid (4.5 hundred million/ml of viable count) got ready is spread with 10% inoculum concentration Enter in above-mentioned mixed raw material, is uniformly mixed;
3, water content 30% or so is controlled, is fermented.Moisture state is kept in fermentation process, water content is observed in timing Variation, is replenished in time moisture.Reach 55 DEG C or more to heap temperature, is carried out turning over throwing with turner, fermentation time is 16 days, i.e., Obtain novel more bacterium composite microbe fertilizers.
Novel more bacterium composite microbe fertilizers that Examples 1 to 3 is prepared are tested:
Test plant: spinach, capsicum and cherry tomato.
Processing 1: blank control
Processing 2: conventional fertilizer application
Processing 3: novel more bacterium composite microbe fertilizers after conventional fertilizer application+application inactivation
Processing 4: the novel more bacterium composite microbe fertilizers of conventional fertilizer application+application
The above processing is repeated 3 times, and experimental plot area is 10m2
Specific fertilizing method:
1, cherry tomato: getting out cherry tomato seedling in advance, uses for transplanting.Start to transplant on September 15th, 2018.Processing 1 (not applying fertilizer), earthing after transplanting;Handle 2 (conventional fertilizer applications): every cell is each October 15, November 5, December 25 It applies 45% compound potassium sulfate fertilizer (20:8:17) 0.6kg and (adds up to every 666.7m three times2Apply 120kg);Handle 3 (conventional fertilizer applications+apply Novel more bacterium composite microbe fertilizers after using inactivation): novel more bacterium composite microbe fertilizers after inactivation are applied as base manure, The every 666.7m on the 15th of September in 20182200kg is applied, plot area (10m is converted2) apply novel more bacterium complex microorganisms after inactivation Fertile 3.0kg.Conventional fertilizer application is according to the progress for handling 2;Handle 4 (conventional fertilizer applications+novel more bacterium composite microbe fertilizers): microorganism Fertilizer is applied as base manure, the every 666.7m on the 15th of September in 20182200kg is applied, plot area (10m is converted2) make microorganism fertilizer 3.0kg, conventional fertilizer application is according to the progress for handling 2.Other way to manages of growth period are consistent, and cherry tomato was January 12 in 2018 Day receives, and surveys biomass.Wherein influence of the different disposal to cherry tomato biological character is as shown in table 1:
Influence of 1 different disposal of table to cherry tomato biological character
Influence of the different disposal to cherry tomato yield is as shown in table 2:
Influence of 2 different disposal of table to cherry tomato yield
By Tables 1 and 2 it is found that cherry tomato root growth is very fast, stalk after the novel more bacterium composite microbe fertilizers of application Sturdy, blade growing way is vigorous, and yield is high, and contrast difference is obvious with other processing, there is apparent effect of increasing production.
2, capsicum (green point green pepper): on March 28th, 2018 to April 2, by 120cm specification ridging, ridge body height 35cm with On.Handle 1 (not applying fertilizer), earthing after transplanting;Handle 2 (conventional fertilizer applications): every cell is applied May 15, July 5 45% compound potassium sulfate fertilizer (20:8:17) 0.6kg (adds up to every 666.7m twice2Apply 80kg);Handle 3 (conventional fertilizer applications+inactivation Novel more bacterium composite microbe fertilizers afterwards): novel more bacterium composite microbe fertilizers after inactivation are base manure application, April 2 in 2018 Day every 666.7m2200kg is applied, plot area (10m is converted2) novel more bacterium composite microbe fertilizer 3.0kg after inactivation are applied, it is conventional Fertilising is according to the progress for handling 2;Handle 4 (the more bacteria microorganism fertilizer of conventional fertilizer application+NEW TYPE OF COMPOSITE): microbial manure is as base Fertilizer application, on April 2nd, 2018 every 666.7m2200kg is applied, plot area (10m is converted2) make microorganism fertilizer 3.0kg.Growth period Other way to manages are consistent, Yu Qiyue harvest capsicum on the 15th, survey capsicum biological character, while observe statistics chilli seedling, The plant number of phytophthora root rot occurs for florescence, fruiting period.Wherein influence of the different disposal to capsicum biological character is as shown in table 1:
Influence of 1 different disposal of table to capsicum biological character
Influence of the different disposal to yield of hot pepper is as shown in table 2:
Influence of 2 different disposal of table to yield of hot pepper
Processing 1 Processing 2 Processing 3 Processing 4
Yield 25.5 34.2 37.8 42.5
By Tables 1 and 2 it is found that capsicum root growth is very fast, and stalk is thick after the novel more bacterium composite microbe fertilizers of application Strong, blade growing way is vigorous, and yield is high, and there is no the plant numbers of phytophthora root rot, and contrast difference is obvious with other processing, has obvious Effect of increasing production.
Spinach: the sowing time was on November 15th, 2018.1 (not applying fertilizer) is handled, thin soil is after planting covered, uses rake It dresses seed with soil;Processing 2 (conventional fertilizer applications): every cell applied November 25, December 5 45% compound potassium sulfate fertilizer (20: 8:17) 0.6kg (adds up to every 666.7m twice2Apply 80kg);Handle 3 (novel more bacterium composite microbials after conventional fertilizer application+inactivation Object fertilizer): novel more bacterium composite microbe fertilizers after inactivation as base manure application, on November 15th, 2018 every 666.7m2It applies 120kg, novel more bacterium composite microbe fertilizer 1.8kg after converting (10 square metres) of plot area application inactivations, conventional fertilizer application According to the progress of processing 2;Handle 4 (conventional fertilizer applications+microbial manure): novel more bacterium composite microbe fertilizers are applied as base manure, On November 15th, 2018 every 666.7m2120kg is applied, plot area (10m is converted2) make microorganism fertilizer 1.8kg, conventional fertilizer application according to The progress of processing 2.Other way to manages of growth period are consistent, spinach harvested on December 20th, 2018, survey yield.Wherein Influence of the different disposal to spinach biological character is as shown in table 1:
Influence of 1 different disposal of table to spinach biological character
Influence with processing to spinach yield is as shown in table 2:
Influence of 2 different disposal of table to spinach yield
Processing 1 Processing 2 Processing 3 Processing 4
Yield 13.2 19.3 23.3 28.8
By Tables 1 and 2 it is found that spinach root growth is very fast, and blade is long after the novel more bacterium composite microbe fertilizers of application Gesture is vigorous, and yield is high, and contrast difference is obvious with other processing, there is apparent effect of increasing production.This may with for examination fertilizer in having The effect of microorganism live bacteria and organic matter is imitated, the bacillus laterosporus and bacillusmusilaginosiengineering in effective microbe viable bacteria can The biological fertility of activating soil, improve root growth environment, promote spinach to fertilizer be absorbed and utilized and disease resistance improve, The growth of spinach can preferably be promoted, to promote the raising of spinach yield.
The present invention provides a kind of novel more bacterium composite microbe fertilizers and its preparation method and application, novel more bacterium Composite microbe fertilizer can promote crop rhizosphere to develop, and both provide nutrition in all directions for crop, moreover it is possible to crop disease-resistant ability is improved, There is good inhibiting effect to the root knot nematode disease of crop, have certain upgrading effect of increasing production to crop.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications It should be regarded as protection scope of the present invention.

Claims (10)

1. a kind of novel more bacterium composite microbe fertilizers, which is characterized in that the preparation method of novel more bacterium composite microbe fertilizers The following steps are included: (1) is by pig manure, seaweed, mushroom slag and stalk according to (35~45): (15~25): (25~35): (5~15) Mass ratio mixing carry out first fermentation, obtain the first fermentation material;
(2) microbial inoculum is inoculated into first fermentation material, is uniformly mixed and carries out the second fermentation, it is compound micro- obtains novel more bacterium Bio-fertilizer;The microbial inoculum includes bacillus laterosporus and bacillusmusilaginosiengineering.
2. novel more bacterium composite microbe fertilizers according to claim 1, which is characterized in that step (1) pig manure, seaweed, The mass ratio of mushroom slag and stalk is (36~43): (16~24): (26~34): (8~13).
3. novel more bacterium composite microbe fertilizers according to claim 1, which is characterized in that step (1) described first, which is fermented, is Fermentation is stacked, the time of first fermentation is 12~18d.
4. novel more bacterium composite microbe fertilizers according to claim 1, which is characterized in that step (2) the side spore gemma bar Bacterium and bacillusmusilaginosiengineering individually carry out the inoculation.
5. novel more bacterium composite microbe fertilizers according to claim 4, which is characterized in that step (2) the gel-shaped gemma Bacillus includes bacillusmusilaginosiengineering No. 01 bacterial strain of three torches, and deposit number is CGMCC NO.3995.
6. according to claim 1 or 4 novel more bacterium composite microbe fertilizers, which is characterized in that the side spore gemma bar The inoculation volume of bacterium is the 10~30% of the first fermentation material volume.
7. novel more bacterium composite microbe fertilizers according to claim 5, which is characterized in that the bacillusmusilaginosiengineering connects Kind volume is 10~15% of the first fermentation material volume after the inoculation bacillus laterosporus.
8. novel more bacterium composite microbe fertilizers according to claim 1, which is characterized in that when step (2) described second is fermented Water content in system is 20~45%.
9. right will go any one of 1~8 novel more bacterium composite microbe fertilizers improving the application in crop yield and quality.
10. applying according to claim 9, which is characterized in that the crop includes spinach, capsicum and tomato.
CN201910262313.7A 2019-04-02 2019-04-02 Novel more bacterium composite microbe fertilizers of one kind and its preparation method and application Pending CN110066194A (en)

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CN111848242A (en) * 2020-07-29 2020-10-30 福建三炬生物科技股份有限公司 Compound microbial agent with biocontrol function and preparation method and application thereof
CN111943774A (en) * 2020-07-14 2020-11-17 湖北绿道农业发展有限公司 Functional biological organic fertilizer and preparation method thereof
CN113402336A (en) * 2021-06-29 2021-09-17 洋浦绿宝丰农资有限公司 Special disease-resistant and growth-promoting fertilizer for betel nuts and preparation method thereof

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CN101863685A (en) * 2010-06-07 2010-10-20 王来福 Method for making high-concentration bacterial powder for microbial fertilizers
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