CN115353986B - Bacillus bailii strain WB strain for treating pig raising wastewater and application thereof - Google Patents
Bacillus bailii strain WB strain for treating pig raising wastewater and application thereof Download PDFInfo
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Abstract
The invention discloses a bacillus bailii strain WB strain for treating pig raising wastewater and application thereof. The bacillus bailii strain WB strain is preserved in China Center for Type Culture Collection (CCTCC) with a strain preservation number of CCTCC NO: m20211547. According to the invention, different kinds of beneficial microorganisms are inoculated into pig raising wastewater to screen and obtain bacillus bailii strain WB strain which grows in the pig raising wastewater and has a relatively high propagation speed, and the utilization condition of nutrient components in the pig raising wastewater and the degradation condition of pollutants in the pig raising wastewater are detected, so that the result shows that WB can grow in the original pig raising wastewater and has relatively high biomass, the biomass is obviously improved after the WB is inoculated by using an optimized culture medium, and the highest viable count is 4.64 multiplied by 10 10 cfu/mL, has better removal rate for COD and ammoniacal nitrogen, and can effectively regulate the pH value of the original pig raising wastewaterValues. The invention reduces the possibility of environmental pollution caused by the pig raising wastewater on one hand and is more beneficial to the resource utilization of the pig raising wastewater on the other hand.
Description
Technical Field
The invention relates to a microorganism strain capable of being used for treating pig raising wastewater, in particular to application of a bacillus beijerinckii strain WB strain in treating pig raising wastewater, and also relates to a method for treating pig raising wastewater. The invention belongs to the technical field of agricultural production.
Background
China is one of the most huge industrial countries for large-scale pig breeding and consumption in the world. Along with the continuous development of economy, the scale of a live pig breeding industry chain is continuously expanded, according to the year of statistics in 2016, the annual pork yield in 2015 of China is 5486.5 ten thousand tons, the live pig breeding scale is huge (national statistical bureau, 2016), and the produced livestock manure also becomes a major pollution source, so that the problem of environmental pollution is also more and more remarkable. The total production of livestock manure in China reaches 2.43x10 8 Ton of urine 1.63x10 8 Ton, total nitrogen 1.02x10 6 Ton, total phosphorus 1.60x10 5 Ton (Pan Qing, 2002), COD discharge amount reaches 7118 ten thousand tons, which is far more than the sum of the discharge amounts of industrial wastewater and domestic wastewater COD, and most of pig farms use flushing water to clean pig houses, so pig farm wastewater, such as pig urine, excrement and the like, are mixed with each other, ammonia nitrogen content, phosphorus content, suspended matters and organic matters in the wastewater are high in concentration and seriously unbalanced in carbon nitrogen ratio, and if a large amount of untreated discharge is discharged to the environment such as lakes, rivers, farms and the like, the water body is enriched, soil properties are changed and other column pollution problems are caused.
At present, the pig raising wastewater treatment technology at home and abroad mainly focuses on three major modes, namely a physical and chemical treatment technology, a natural treatment technology and a biological treatment technology. Among the methods used in the physicochemical treatment are a medium adsorption method, a flocculation precipitation method and the like, such as Qian Feng, the zeolite-straw combination is used for filtering the swine waste water, and the removal rates of COD, ammoniacal nitrogen and phosphorus can reach 47.9%, 72.9% and 50.1% (Qian Feng, 2008) respectively, and 61.02% of swine waste water with the COD concentration of 3232mg/L can be removed by a magnetic flocculation method like Cui Lina and the like (Cui Lina, 2010). Although the physicochemical method has better pollutant removal rate on the swine waste water, the physicochemical method has the disadvantages of lower broad spectrum, higher pollutant removal cost, larger investment of treatment equipment, less engineering application and the like on the swine waste water pollution removal. The natural treatment method generally utilizes the comprehensive action of natural soil, water body and organisms to remove pollutants, such as Zhu Xizhen, and the like, and the artificial wetland construction is carried out through blast furnace slag, quartz sand and other matrixes, so that the removal rates of COD, BOD and phosphorus respectively reach 71% -88%, 80% -89%, 70% -85% (Zhu Xizhen, 2003), and Lu Xiuguo and the like, and the pig raising wastewater COD is less than or equal to 400mg/L and ammonia nitrogen is less than or equal to 70mg/L (Lu Xiuguo, 2009) by utilizing the oxidation pond. The natural treatment method has better decontamination capability on polluted wastewater, but has insufficient stability and longer purification time. The biological treatment technology is a novel method for treating high-concentration organic wastewater by using the catalysis of microorganisms, such as Liang Meidong, and the like, which uses an SRB reactor to treat the pig raising wastewater by aeration can increase the removal rate of COD to more than 90% (Liang Meidong, 2009), and Li Fengmin, and the like, can enable the removal rate of ammoniacal nitrogen and total nitrogen to reach 99.7% and 50.7% by adopting an aerobic-anaerobic combined treatment method (Li Fengmin, 2011). However, a large amount of activated sludge is generated in the process of treating the wastewater by a biological treatment method, and cannot be treated, so that secondary pollution is caused to the environment.
Although the pig raising waste water causes huge pressure on the environment, the pig raising waste water contains a great amount of organic matters, nitrogen, phosphorus, potassium and other nutrient elements. In the first Chinese public inspection notice of pollution in 2010, it is clearly pointed out that total nitrogen and total phosphorus are the foremost pollutant emissions of the livestock and poultry raising industry, the emissions are 102.48 ten thousand tons and 16.04 ten thousand tons respectively, and the total nitrogen and total phosphorus emissions in the national water pollution respectively account for 21.7% and 37.9%. Therefore, the feces and the wastewater generated by livestock are very important resources, and the potential of recycling is huge. If the pig raising waste water is used as a basic culture medium for fermentation production of beneficial microorganisms, the production cost of the microbial fertilizer can be greatly reduced, and the pollution problem can be reduced through biodegradation, so that the waste water can be changed into valuable, and the residual value of the waste water is realized.
Therefore, the invention takes the pig raising wastewater as a natural screening culture medium to carry out strain screening, selects the high-efficiency strain Bacillus velezensis WB (CCTCC NO: M20211547) which can efficiently utilize the nutrient components of the pig raising wastewater for self growth to carry out experiments, and researches the utilization condition of the pig raising wastewater and the treatment condition of pollutants so as to provide technical support for harmless resource utilization of the pig raising wastewater.
Disclosure of Invention
One of the objects of the present invention is to provide a microorganism strain useful for treating swine wastewater;
the second object of the invention is to provide a method for treating pig raising wastewater.
In order to achieve the above purpose, the invention adopts the following technical means:
according to the invention, different kinds of beneficial microorganisms are inoculated into pig raising wastewater to screen and obtain bacillus bailii strain (Bacillus velezensis) WB strain (CCTCC NO: M20211547) which grows in the pig raising wastewater and has a relatively high propagation speed, and the utilization condition of nutrient components in the pig raising wastewater and the degradation condition of pollutants in the pig raising wastewater are detected, so that the bacillus bailii strain WB can grow in the original pig raising wastewater and has relatively high biomass, the biomass is obviously improved after the optimized culture medium is used for inoculating WB, and the highest viable count is 4.64 multiplied by 10 10 cfu/mL. The pig raising waste water culture medium with optimized carbon nitrogen ratio is fermented to have good removal rate of COD and ammoniacal nitrogen, the removal rate of COD is 82%, the removal rate of ammoniacal nitrogen is 76.33%, the pig raising waste water has certain phosphorus content removal capacity, the removal rate is 35.88%, the pH value of the original pig raising waste water can be effectively regulated, the possibility of environmental pollution caused by fermentation waste liquid is reduced, and the recycling utilization of the pig raising waste water is better promoted.
Based on the research, the invention firstly provides a bacillus belicus strain (Bacillus velezensis) which can be used for treating pig raising wastewater, and is named Bacillus velezensis WB, classified and named Bacillus velezensis WB respectively, and preserved in China center for type culture collection, with the address of China, university of Wuhan, and with the strain preservation number of CCTCC NO: m20211547, storage time is 2021, 12 and 6.
Furthermore, the invention also provides application of the bacillus beijerinckii strain in treating pig raising wastewater.
Preferably, the seed solution of the bacillus belicus strain is inoculated into sterilized pig raising wastewater to be treated, the treated pig raising wastewater can be obtained after fermentation, and the treated pig raising wastewater has reduced pollutant content compared with the treated pig raising wastewater.
Wherein, preferably, the method further comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
wherein, preferably, the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
Preferably, the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus in the treated pig raising wastewater is reduced compared with the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus before treatment.
Furthermore, the invention also provides a method for treating the pig raising wastewater, which comprises the following steps:
(1) Inoculating the bacillus bailii strain WB into a beef extract peptone culture medium for activation, multiplexing the activated strain WB into the beef extract peptone culture medium, and then adjusting the concentration of the strain to OD by using sterile water 600 =1.0 as seed fluid;
(2) Inoculating the seed liquid into sterilized pig raising waste water to be treated, and fermenting to obtain treated pig raising waste water, wherein the treated pig raising waste water has lower pollutant content than the treated pig raising waste water.
Wherein, preferably, the step (2) further comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
wherein, in the step (2), seed liquid is inoculated into sterilized pig raising wastewater to be treated according to the inoculum size of 1-2 vol%; the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
Preferably, the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus in the treated pig raising wastewater is reduced compared with the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus before treatment.
Compared with the prior art, the invention has the beneficial effects that:
the invention realizes the recycling utilization of pig raising wastewater by using beneficial microorganisms, the technology is based on a biological treatment method, and the core of the technology is to remove pollutants from pig raising wastewater by using a microorganism aerobic-anaerobic combined treatment method, and the invention creatively utilizes rich nutrition in pig raising wastewater to realize mass propagation of beneficial microorganisms so as to obtain beneficial microorganism thalli for preparing microbial agents, and creates economic value while removing pollutants to protect the environment. The invention takes pig raising waste water as a natural culture medium to culture microorganisms, so the pig raising waste water is selected because a large amount of organic matters, carbon sources, nitrogen sources and other nutrient substances contained in the pig raising waste water can provide sufficient nutrients for the growth of microorganisms, the natural temperature bed for the culture of microorganisms is adopted, and substances harmful to the environment in the pig raising waste water can be absorbed, converted and fixedly used for the proliferation of thalli and the secretion of metabolites in the microorganism growth process, so that on one hand, the pollution sources such as ammonia nitrogen, organic matters, phosphorus and the like which are caused to the environmental safety in the pig raising waste water are reduced, on the other hand, a large amount of microorganism thalli which are cultured by the pig raising waste water can be collected for various microbial preparations such as plant promotion, crop biocontrol, pollutant degradation and the like, the cost of the culture medium of microorganisms is high in the culture process, one of main reasons for causing the price of the microorganism microbial inoculum is high, and the use of the pig raising waste water is close to the beneficial microorganism strains in viable count to the traditional culture medium, but the cost is greatly reduced, and the green and the agricultural microbial inoculum is greatly reduced.
The invention carries out carbon nitrogen ratio optimization and adjustment on pig raising wastewater and then inoculatesThe beneficial microorganisms are screened to finally obtain 1 strain bacillus bailii strain (Bacillus velezensis) WB strain (CCTCC NO: M20211547) which grows in pig raising wastewater and has high propagation speed, the bacillus bailii strain WB can grow in the original pig raising wastewater and has high biomass, the biomass is obviously improved after the optimized culture medium is used for inoculating WB, and the highest viable count is 4.64 multiplied by 10 10 cfu/mL. The pig raising waste water culture medium with optimized carbon nitrogen ratio is fermented to have good removal rate of COD and ammoniacal nitrogen, the removal rate of COD is 82%, the removal rate of ammoniacal nitrogen is 76.33%, the pig raising waste water has certain phosphorus content removal capacity, the removal rate is 35.88%, the pH value of the original pig raising waste water can be effectively regulated, the possibility of environmental pollution caused by fermentation waste liquid is reduced, and the recycling utilization of the pig raising waste water is better promoted.
Therefore, the beneficial microorganism realizes the pig raising wastewater recycling utilization, the decontamination capability of the pig raising wastewater is not weaker than that of the main stream physicochemical treatment technology, the natural treatment technology and the traditional biological treatment technology at home and abroad, the effect is even better, and the technology has higher broad spectrum on the types of pollutant removal in the pig raising wastewater more comprehensively; the pig raising wastewater treatment by microbial fermentation is lower in decontamination cost, equipment investment is low, a large number of sites are not needed, decontamination period is short, small fermentation equipment can be quickly built near the source to treat the pig raising wastewater, and a small enterprise can also bear the system construction cost; the beneficial microorganism is used for fermentation, so that the beneficial microorganism strain can be obtained for the production and use of the microbial agent while the pig raising wastewater is treated, the damage of pollutants to the environment is reduced, the economic benefit is reported, the promotion of the microbial agent is promoted, the development of green agriculture is accelerated, and a virtuous circle system of ecological protection-agricultural development is developed, so that the sustainable development strategy of China is met.
Drawings
FIG. 1 shows the change of the biomass in swine wastewater after the initial and optimized carbon-nitrogen ratios of WB strain with time;
FIG. 2 shows the change in total organic carbon content and the reduction rate (A) of the raw (B) treated by the WB strain compared with the optimized pig raising wastewater (C);
FIG. 3 shows the total carbon content change and the reduction rate (A) of the raw (B) treated by the WB strain compared with the optimized pig raising wastewater (C);
FIG. 4 shows the total nitrogen content change and the reduction rate (A) of the raw (B) treated by the WB strain compared with the optimized pig raising wastewater (C);
FIG. 5 shows the changes of total nitrogen, total carbon, total organic carbon and COD in the supernatant of the fermentation broth of WB strain;
FIG. 6 shows the pH change of WB strain in pig raising waste water and the ammonia nitrogen and phosphorus eliminating rate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in connection with the embodiments of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present invention are within the protection scope of the present invention.
Example 1 screening of Strain and its application in treatment of pig raising wastewater
1 materials and methods
1.1 sources of test materials
The strains used in the test are all different pure culture beneficial microorganisms obtained by topic separation and screening, and researches find that each strain is a high-efficiency strain with better industrialized application potential in the aspects of plant growth promotion, biological control, pollutant control and the like, and the strains are preserved in a refrigerator at the temperature of minus 80 ℃ for standby, and all strains are identified by strain morphology and 16SrDNA classification and are WZW03 (plant sp.), LZN01 (Bacillus amyloliquefaciens), WA (Bacillus velezensis), WC (Bacillus methylotrophilus), WB (Bacillus velezensis), JDY (Bacillus mucilaginosus) and CL01 (Sphingomonas sp.). The pig raising waste water is taken from a pig raising farm in the Fula base region of zizihar, and the collected pig raising waste water is stored at 4 ℃ for later experiments, wherein the basic characteristics of the pig raising waste water are 403.2mg/L total nitrogen, 981.6mg/L total carbon, 361.9mg/L total organic carbon, 48.66mg/L phosphorus content and 315.98mg/L, pH 8.85.85 ammonia nitrogen.
1.2 Medium
Beef extract peptone medium: 10.00g of peptone, 3.00g of beef extract, 5.00g of NaCl, 20.00g of agar, and distilled water with a constant volume of 1000.00mL, wherein agar is not added into the liquid culture medium.
Original pig raising wastewater culture medium: pig raising waste water from pig farm transportation.
Pig raising wastewater culture medium with optimized carbon-nitrogen ratio: 10g of brown sugar, and the volume of the pig raising wastewater is fixed to 1000.00mL, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
1.3 high-efficient degradation Strain preliminary screening of pig raising wastewater
1.3.1 seed liquid preparation
The test strains are respectively inoculated to beef extract peptone culture medium and cultured for 24 hours at the temperature of 30 ℃ for activation. The activated strains were multiplexed in a beef extract peptone medium, shake-cultured at 30℃and 120r/min, and each strain cultured to the same concentration (OD 600 = 1.0) was used as a seed solution for a treatment test of swine wastewater.
1.3.2 Strain Primary screening
In order to screen strains capable of growing in a large amount in pig raising wastewater, seed solutions of different strains are inoculated according to an inoculation amount of 1vol%, shake flask culture is carried out at 30 ℃ and 120r/min, fermentation liquor is sampled every 24h, the number of viable bacteria in each time period is detected to determine the maximum viable bacteria amount of the strains, and then, strains with higher fermentation biomass are selected for subsequent experiments.
1.4 exploration of the effect of screening strains on the utilization of pig raising wastewater
1.4.1 screening of growth conditions of strains in raw wastewater and optimization of carbon-nitrogen ratio in wastewater and treatment effect change
Because the carbon-nitrogen ratio in the pig raising wastewater stock solution is not the optimal carbon-nitrogen ratio for the growth of thalli, the growth and propagation of the pig raising wastewater stock solution can be influenced to reduce the treatment effect of the pig raising wastewater, so that the pig raising wastewater culture medium is subjected to carbon-nitrogen ratio optimization, whether the thallus content can be improved or not is detected, and the treatment capacity of the pig raising wastewater is enhanced. The dominant strains obtained after screening are respectively inoculated into a beef extract peptone culture medium to prepare seed liquid, the seed liquid is respectively inoculated into 300mL of an original pig raising wastewater culture medium and an optimized culture medium added with 1wt% of brown sugar according to the inoculum size of 2vol%, the culture medium is subjected to shake flask culture at 30 ℃ and 120r/min, and samples are sampled and measured for each index every 24 hours.
1.4.2 determination of the indices of the centrifugal fermentation liquor of the pig waste Water after optimization of the carbon-to-nitrogen ratio by the treatment of the screening Strain
In order to simulate whether the pig raising waste liquid treated by various strains after the thalli are acquired in the industrial production process can further reduce the potential of environmental threat, the experiment carries out index detection after centrifuging sample liquids with different fermentation time. Inoculating the screened strain into an optimized culture medium, culturing at 30 ℃ under 120r/min shaking, sampling samples every 24h, centrifuging for 5min under the condition of 12000rpm, and then measuring indexes.
1.5 test measurement index
1.5.1 Water quality index
And (3) measuring COD, pH, total nitrogen, total carbon, total organic carbon, ammoniacal nitrogen and phosphorus content of the sample.
1.5.2 microbial indicators
10mL of fermentation broth is put into a shaking table with 90mL of sterile water at 200r/min to shake for 30min, and then gradient dilution is carried out to obtain 10 -7 、10 -8 、10 -9 And (3) taking 0.1mL of the diluted solution, coating the diluted solution on a beef extract peptone culture medium plate, culturing at 30 ℃ for 24 hours, and calculating the viable count (cfu/mL) to form three groups of parallel groups.
2. Results and discussion
2.1 screening of strains and comparison of growth of screened strains in original and optimized pig raising wastewater Medium over time
The aim of screening different strains is fulfilled by detecting the maximum content of the viable count of each strain in the pig raising wastewater, and the beneficial strains are cultivated by using the pig raising wastewater to find that WB can grow in the pig raising wastewater and has higher biomass, and the maximum viable count is 2.28x10 10 cfu/mL。
The bacillus belicus strain (Bacillus velezensis) which can be used for treating pig raising wastewater is named as WB and is preserved in China center for type culture collection, the address is in university of Wuhan, china, and the strain preservation number is CCTCC NO: m20211547, storage time is 2021, 12 and 6.
To determine the biomass of WB in different media, viable count assays were performed by inoculating to the original and optimized media, respectively. As can be seen from FIG. 1, the WB strain in the original medium reached a maximum biomass of 2.28X10 after 48h 10 cfu/mL, and the maximum biomass in brown sugar is 4.64×10 10 cfu/mL, the biomass is improved by 50.86 percent.
2.2WB grows the change condition of the total organic carbon, the total carbon and the total nitrogen of the fermentation liquor in the original and optimized pig raising wastewater culture medium
2.2.1 comparing the treatment conditions of the total organic carbon in the original and optimized pig raising wastewater culture media
As can be seen from fig. 2, after the WB strain is inoculated, the total organic carbon content in the fermentation broth of the original pig raising wastewater shows a tendency of increasing and then decreasing with the increase of the culture time of the thalli, the minimum total organic carbon content after WB fermentation is 258.7mg/L compared with the control group, the total organic carbon reduction rate is 28.52%, the total organic carbon content of the fermentation broth reaches the minimum value when the WB is inoculated in the culture medium after optimizing the carbon nitrogen ratio with the increase of the culture time, the WB reduction rate reaches 57.53% compared with the control group, and the optimized culture medium consumes more total organic carbon and consumes more rapid compared with the original pig raising wastewater culture medium.
2.2.2 comparing the total carbon treatment conditions in the original and optimized pig raising wastewater media
And (3) respectively inoculating WB into the original pig raising wastewater culture medium and the optimized pig raising wastewater culture medium, and measuring total carbon of the fermentation liquor every 24 hours. As shown in fig. 3, after the WB strain was inoculated to the original swine wastewater medium, the total carbon content in the fermentation broth showed a tendency to slowly decrease and then to stabilize with increasing culture time, the minimum value to which the total carbon content of the WB fermentation broth decreased was 769.5mg/L, and the total carbon reduction rate of the WB fermentation broth of the selected strain was 21.61% compared to the control group. After the screened strain is inoculated to the optimized pig raising waste water culture medium, the total carbon content in the fermentation broth is continuously reduced along with the improvement of the culture time, the speed is higher, the fermentation broth finally tends to be slowly reduced, the total carbon content of the fermentation broth reaches the minimum value WB=2360 mg/L after 96 hours of culture, and the total carbon reduction rate is reduced by 54.17 percent compared with a control group. The WB is cultivated in the optimized culture medium, and the total carbon content reduction rate in the fermentation broth is obviously improved compared with that in the original pig raising wastewater.
2.2.3WB Total Nitrogen modification in raw and optimized pig raising wastewater Medium
And (3) respectively inoculating WB into the original pig raising wastewater culture medium and the optimized pig raising wastewater culture medium, and measuring total nitrogen of the fermentation liquor every 24 hours. As can be seen from FIG. 4, the total nitrogen content of the fermentation broth after the WB strain was inoculated with the original swine waste water generally showed a continuous decrease trend with the increase of the strain fermentation time, and the total nitrogen content of the WB fermentation broth was reduced to a minimum value of 179.2mg/L at 96h of cultivation, compared with the control group, the total nitrogen content was reduced by 55.56%. When the carbon nitrogen ratio of the waste water from pig raising is regulated and optimized, the total nitrogen content of the WB fermentation broth also shows a general decline trend along with the extension of the fermentation time, and the total nitrogen content is rapidly declined in the fermentation interval of 0-24h, then the total nitrogen content is gradually gentle, the total nitrogen content reaches the minimum value of 146.6mg/L after the WB is cultivated for 96h, and compared with the total nitrogen content of the WB in the control group, the total nitrogen content is declined by 56.11%. The comparison shows that the reduction rate of the total nitrogen of the fermentation liquor of the WB grown in the original and optimized pig raising wastewater culture media has no significant difference.
2.4 Change of various indicators of fermentation waste liquid after removing thalli from pig raising waste water treated by WB
The comparison shows that the WB strain can grow and reproduce better in the optimized pig raising waste water culture medium, so that the optimized pig raising waste water culture medium is selected as a basic culture medium for industrial fermentation of beneficial microorganisms, and the change condition of pollutants in filtered waste liquid after the thallus is obtained is simulated and detected in industrial production so as to explore the degradation capability of the strain on the pollutants.
2.4.1WB variation of total nitrogen, total carbon, total organic carbon and COD content in optimized pig raising wastewater
And (3) inoculating WB into the optimized pig raising wastewater culture medium, and measuring total nitrogen, total carbon and total organic carbon of the supernatant after centrifugation every 24 hours. As shown in fig. 5, compared with the control group, the total nitrogen content in the supernatant of the centrifuged WB fermentation broth is reduced by 93.87%, the total carbon content is reduced by 75.53%, and the total organic carbon content is reduced by 75.06%, which indicates that the WB strain can better utilize the nutrients in the pig raising wastewater to perform its own growth, propagation and metabolism activities, and prevent the filtered fermentation waste from being rich in nutrients, thereby causing environmental pollution. In order to determine the COD removal effect of the screening strain on the pig raising wastewater, the COD detection is carried out on the supernatant fluid of the WB fermentation broth, and as the fermentation time of the strain is continuously increased, the COD content of the strain is continuously and rapidly reduced, and the maximum COD removal rate reaches 82%, which indicates that the WB has better removal rate on the COD and reduces the threat of filtered waste liquid on the environment.
2.4.2WB pH change and ammonia nitrogen and phosphorus removal rate in optimized pig raising waste water culture medium
The pH measurement was performed every 24 hours for the WB fermentation broth, and it was found from fig. 6 that the pH of the swine waste water was reduced from the original alkaline solution having ph=8.85 to the acidic fermentation broth having ph=5.86 after the WB strain was fermented for 96 hours, which may be caused by the secretion of organic acid during the strain growth. As shown in FIG. 6, the WB strain can significantly reduce the content of ammonia nitrogen in the fermentation waste liquid, the ammonia nitrogen removal rate reaches 76.33%, and the ammonia nitrogen removal effect is good. The phosphorus content of the supernatant fluid of the fermentation liquor is measured, and compared with the control group WB, the phosphorus removal rate of the supernatant fluid of the fermentation liquor can reach 35.88%, so that the supernatant fluid of the fermentation liquor has a certain phosphorus removal effect.
Conclusion 3
(1) The WB can grow in the original pig raising waste water and has higher biomass, the biomass is obviously improved after the WB is inoculated by using the optimized culture medium, and the highest viable count is 4.64 multiplied by 10 10 cfu/mL。
(2) The WB strain is fermented in the pig raising wastewater culture medium with optimized carbon nitrogen ratio, has better removal rate of COD and ammoniacal nitrogen, has 82% removal rate of COD and 76.33% removal rate of ammoniacal nitrogen, has certain phosphorus content removal capacity on pig raising wastewater, has 35.88% removal rate, can effectively adjust the pH value of the original pig raising wastewater, reduces the possibility of environmental pollution caused by fermentation waste liquid, and better promotes the recycling utilization of the pig raising wastewater.
Claims (10)
1. Bacillus bailii (Bacillus velezensis) strain for treating pig raising waste water, named Bacillus velezensis WB, is preserved in China center for type culture collection, and has a strain preservation number of CCTCC NO: m20211547, storage time is 2021, 12 and 6.
2. Use of the bacillus belicus strain as claimed in claim 1 for the treatment of swine wastewater.
3. The use according to claim 2, wherein the seed solution of bacillus bescens strain is inoculated into sterilized swine waste water to be treated, and the treated swine waste water is obtained after fermentation, and the treated swine waste water has reduced pollutant content compared with the swine waste water before treatment.
4. The use according to claim 3, further comprising the step of adding brown sugar to the sterilized swine waste water to be treated such that the swine waste water has a carbon to nitrogen ratio of 16-20:1.
5. the use according to claim 3, wherein the fermentation is carried out at 30℃and 120r/min for 24-96 hours.
6. The use according to claim 3, wherein the treated swine wastewater has reduced levels of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus as compared to the pre-treatment swine wastewater.
7. A method for treating pig raising wastewater, comprising the steps of:
(1) The belais bud of claim 1Inoculating the bacillus strain into beef extract peptone culture medium for activation, multiplexing the activated strain into beef extract peptone culture medium, and regulating strain concentration to OD with sterile water 600 =1.0 as seed fluid;
(2) Inoculating the seed liquid into sterilized pig raising waste water to be treated, and fermenting to obtain treated pig raising waste water, wherein the treated pig raising waste water has lower pollutant content than the treated pig raising waste water.
8. The method of claim 7, wherein the step (2) further comprises the step of adding brown sugar to the sterilized swine waste water to be treated such that the swine waste water has a carbon to nitrogen ratio of 16-20:1.
9. the method according to claim 7, wherein in the step (2), the seed solution is inoculated into the sterilized waste water of pig raising to be treated in an inoculum size of 1 to 2 vol%; the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
10. The method of claim 7, wherein the treated swine wastewater has reduced levels of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus, and pH as compared to the pre-treatment swine wastewater.
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| CN115353986B (en) * | 2022-01-28 | 2023-07-07 | 齐齐哈尔大学 | Bacillus bailii strain WB strain for treating pig raising wastewater and application thereof |
| CN117586929B (en) * | 2024-01-19 | 2024-03-26 | 南京万瑞环境科技有限公司 | Degradation strain and application thereof in cultivation wastewater treatment |
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