CN115353986A - Bacillus belgii strain WB strain for treating swine wastewater and application thereof - Google Patents
Bacillus belgii strain WB strain for treating swine wastewater and application thereof Download PDFInfo
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Abstract
The invention discloses a Bacillus belgii strain WB strain for treating pig raising wastewater and application thereof. The Bacillus belgii strain WB strain is preserved in China center for type culture collection, and the preservation number of the strain is CCTCC NO: m20211547. According to the invention, different kinds of beneficial microorganisms are inoculated to the pig raising wastewater to screen the WB strain of the Bacillus beiLeisi which grows in the pig raising wastewater and has higher propagation speed, the utilization condition of the WB strain to the nutrient components in the pig raising wastewater and the degradation condition of pollutants in the pig raising wastewater are detected, and the result shows that the WB can grow in the original pig raising wastewater and has higher biomass, and the biomass is obviously improved after the WB is inoculated by using the optimized culture mediumThe highest viable count is 4.64X 10 10 cfu/mL, has better removal rate to COD and ammoniacal nitrogen, and can effectively adjust the pH value of the original pig-raising wastewater. The invention reduces the possibility of environmental pollution caused by the pig raising wastewater and is more beneficial to resource utilization of the pig raising wastewater.
Description
Technical Field
The invention relates to a microbial strain capable of being used for treating swine wastewater, in particular to application of a Bacillus belgii strain WB strain in treatment of swine wastewater, and also relates to a method for treating swine wastewater. The invention belongs to the technical field of agricultural production.
Background
China is one of the most huge pig scale breeding and consumption industry countries in the world. With the continuous development of economy, the scale of a live pig breeding industrial chain is continuously enlarged, according to the annual statistics yearbook in 2016, the annual pork yield of 5486.5 ten thousand tons in 2015 in China, the live pig breeding scale is very huge (national statistics bureau, 2016), and the produced livestock excrement also becomes a main pollution source, so that the environmental pollution problem caused by the livestock excrement is more and more prominent. The total production of livestock and poultry manure in China now reaches 2.43x10 8 Ton, 1.63x10 urine 8 Ton, total nitrogen 1.02x10 6 Ton, total phosphorus 1.60x10 5 The discharge amount of COD of the pig farm wastewater reaches 7118 ten thousand tons (Pan Qing, 2002), which is far more than the sum of the discharge amounts of industrial wastewater and domestic wastewater COD, and most pig farms are cleaned by flushing water, so that pig urine, excrement and the like in the pig farm wastewater are mixed with each other, the concentration of ammonia nitrogen, phosphorus, suspended matters and organic matters in the wastewater is high, and the carbon-nitrogen ratio is seriously unbalanced, and if a large amount of untreated wastewater is discharged to lakes, rivers, farmlands and other environments, the water eutrophication, soil property change and other pollution problems are caused.
At present, the pig-raising wastewater treatment technology at home and abroad mainly focuses on three main types of modes, namely a physicochemical treatment technology, a natural treatment technology and a biological treatment technology. The physical and chemical treatment methods are mostly used as medium adsorption methods, flocculation precipitation methods and the like, for example, qian Feng and the like are used for filtering swine urine wastewater by utilizing zeolite-straw combination, the removal rates of COD, ammonia nitrogen and phosphorus can respectively reach 47.9%, 72.9% and 50.1% (Qian Feng, 2008), and people like Cui Lina and the like can remove 61.02% (Cui Lina, 2010) of swine wastewater with COD concentration of 3232mg/L by a magnetic flocculation method. Although the physicochemical method has better pollutant removal rate on the swine urine wastewater, the physicochemical method has the disadvantages of lower broad spectrum, higher pollutant removal cost, larger treatment equipment investment, less engineering application and the like on the pollution removal of the swine urine wastewater. The natural treatment method generally utilizes natural soil, water and organisms to carry out comprehensive utilization for removing pollutants, such as Zhu Xian and the like, and the removal rates of COD, BOD and phosphorus respectively reach 71-88%, 80-89% and 70-85% (Zhu Xian, 2003) by carrying out artificial wetland construction through substrates such as blast furnace slag and quartz sand and the like, and Lu Xiuguo and the like utilize oxidation ponds to treat pig-raising wastewater COD less than or equal to 400mg/L and ammonia nitrogen less than or equal to 70mg/L (Lu Xiuguo, 2009). The natural treatment method has good decontamination capability on polluted wastewater, but has insufficient stability and long purification time. The biological treatment technology is a novel method for treating high-concentration organic wastewater by using the catalytic action of microorganisms, such as Liang Meidong and the like, the removal rate of COD can be increased to more than 90% by using an SRB reactor for aeration treatment of pig raising wastewater (Liang Meidong, 2009), li Fengmin and the like, and the removal rate of ammonia nitrogen and total nitrogen can reach 99.7% and 50.7% by using an aerobic-anaerobic combined treatment method (Li Fengmin, 2011). However, in the process of treating wastewater by biological treatment, a large amount of activated sludge is generated and cannot be treated, so that secondary pollution is caused to the environment.
Although the pig raising wastewater exerts great pressure on the environment, the pig raising wastewater contains a large amount of organic matters and nutrient elements such as nitrogen, phosphorus, potassium and the like. In the first general Chinese pollution survey announcement in 2010, it is clearly indicated that total nitrogen and total phosphorus are the most important pollutant emissions in the livestock and poultry breeding industry, the emission amounts of the total nitrogen and the total phosphorus are 102.48 ten thousand tons and 16.04 ten thousand tons respectively, and the emission amounts of the total nitrogen and the total phosphorus in the national water pollution account for 21.7 percent and 37.9 percent respectively. Therefore, the excrement and the waste water generated by the livestock are very important resources, and the potential of resource utilization is huge. If the pig-raising wastewater is used as a basic culture medium for beneficial microbial fermentation production, the production cost of the microbial fertilizer can be greatly reduced, and the pollution problem can be reduced through biodegradation, so that the wastewater can be changed into valuable, and the residual value of the wastewater is realized.
Therefore, the method takes the pig wastewater as a natural screening culture medium to screen strains, selects the efficient strain Bacillus velezensis WB (CCTCC NO: M20211547) which can efficiently utilize the nutrient components of the pig wastewater for self growth to test, and researches the utilization condition of the pig wastewater and the pollutant treatment condition so as to provide technical support for the harmless resource utilization of the pig wastewater.
Disclosure of Invention
One of the purposes of the invention is to provide a microbial strain which can be used for treating pig wastewater;
the invention also aims to provide a method for treating the pig-raising wastewater.
In order to achieve the purpose, the invention adopts the following technical means:
according to the invention, different kinds of beneficial microorganisms are inoculated to the pig raising wastewater to screen and obtain a Bacillus velezensis (CCTCC NO: M20211547) WB strain (CCTCC NO: M5363) which grows in the pig raising wastewater and has a higher propagation speed, the utilization condition of the Bacillus velezensis WB strain on nutrient components in the pig raising wastewater and the degradation condition of pollutants in the pig raising wastewater are detected, and the result shows that the Bacillus velezensis WB strain can grow in the original pig raising wastewater and has higher biomass, the biomass is obviously improved after the WB is inoculated by using an optimized culture medium, and the highest viable count is 4.64 multiplied by 10 10 cfu/mL. Fermentation is carried out in a pig raising wastewater culture medium with an optimized carbon-nitrogen ratio, so that the removal rate of COD and ammonia nitrogen is better, the removal rate of COD is 82%, the removal rate of ammonia nitrogen is 76.33%, the pig raising wastewater has certain phosphorus content removal capacity, the removal rate is 35.88%, the pH value of the original pig raising wastewater can be effectively adjusted, the possibility of environmental pollution caused by fermentation waste liquid is reduced, and the resource utilization of the pig raising wastewater is better improved.
On the basis of the research, the invention firstly provides a Bacillus velezensis strain (named as Bacillus velezensis WB) which is classified and named as Bacillus velezensis WB and is preserved in China center for type culture collection and addressed to Wuhan university in China, wherein the strain preservation number is CCTCC NO: m20211547 with a preservation time of 2021, 12 and 6 days.
Furthermore, the invention also provides application of the Bacillus beiLeisi strain in treating pig raising wastewater.
Preferably, the seed solution of the bacillus beleisi strain is inoculated into the sterilized wastewater to be treated for pig raising, and the treated wastewater for pig raising can be obtained through fermentation, wherein the content of pollutants in the treated wastewater for pig raising is reduced compared with that in the wastewater before treatment.
Preferably, the method also comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
wherein, preferably, the fermentation refers to fermentation culture at 30 ℃ and 120r/min for 24-96h.
Preferably, the pig raising wastewater after treatment has reduced contents of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus compared with the pig raising wastewater before treatment.
Furthermore, the invention also provides a method for treating the pig raising wastewater, which comprises the following steps:
(1) Inoculating the Bacillus belgii strain WB into a beef extract peptone culture medium for activation, re-inoculating the activated strain WB into the beef extract peptone culture medium, and adjusting the strain concentration to OD by using sterile water 600 =1.0 as seed liquid;
(2) Inoculating the seed solution into sterilized wastewater to be treated for pig raising, and fermenting to obtain the treated wastewater for pig raising, wherein the content of pollutants in the treated wastewater for pig raising is reduced compared with that before treatment.
Preferably, the step (2) further comprises the step of adding brown sugar into the sterilized swine wastewater to be treated, so that the carbon-nitrogen ratio of the swine wastewater is 16-20:1.
wherein, in the step (2), the seed liquid is preferably inoculated into the sterilized pig raising wastewater to be treated according to the inoculation amount of 1-2 vol%; the fermentation refers to fermentation culture at 30 ℃ and 120r/min for 24-96h.
Preferably, the pig raising wastewater after treatment has reduced contents of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus compared with the pig raising wastewater before treatment.
Compared with the prior art, the invention has the beneficial effects that:
the invention realizes resource utilization of pig raising wastewater by using beneficial microorganisms, and the technology is based on a biological treatment method, and has the core that the pollutant of the pig raising wastewater is removed by using a microbial aerobic-anaerobic combined treatment method, and the mass propagation of the beneficial microorganisms is realized by creatively using rich nutrition in the pig raising wastewater to obtain beneficial microorganism bacteria for preparing a microorganism bacteria agent, so that the pollutant is removed, the environment is protected, and the economic value is created. The invention takes the pig-raising wastewater as a natural culture medium to culture microorganisms, and the selected pig-raising wastewater is a natural hotbed for culturing the microorganisms because a large amount of nutrient substances such as organic matters, carbon sources, nitrogen sources and the like contained in the pig-raising wastewater can provide sufficient nutrients for the growth of the microorganisms, and can absorb, convert and fix substances harmful to the environment in the pig-raising wastewater in the microbial growth process for the multiplication of thalli and the secretion of metabolites, so that on one hand, the sources threatening the environmental safety in the pig-raising wastewater such as ammonia nitrogen, organic matters, phosphorus and the like are reduced, on the other hand, a large amount of microbial thalli cultured by the pig-raising wastewater can collect microbial preparations used in various aspects such as plant promotion, crop biocontrol, pollutant degradation and the like, thereby achieving two purposes, the higher cost of the culture medium of the microorganisms in the culture process is one of the main reasons for causing the price of the microbial inoculant to be higher, while the use of the pig-raising wastewater to culture beneficial microbial strains on the viable count is close to the traditional culture medium, but the cost is greatly reduced, and the important significance is realized on the green fertilizer for realizing the popularization of the microbial inoculant.
The invention carries out carbon nitrogen ratio optimization adjustment on the pig raising wastewater, inoculates beneficial microorganisms, and finally obtains 1 strain Bacillus beleisis strain which grows in the pig raising wastewater and has higher reproduction speed through screening (Bacillus velezensis) WB strain (CCTCC NO: m20211547), bacillus belgii strain WB can grow in original pig raising wastewater and has high biomass, the biomass is obviously improved after the WB is inoculated by using an optimized culture medium, and the highest viable count is 4.64 multiplied by 10 10 cfu/mL. Fermentation is carried out in a pig raising wastewater culture medium with an optimized carbon-nitrogen ratio, so that the removal rate of COD and ammonia nitrogen is better, the removal rate of COD is 82%, the removal rate of ammonia nitrogen is 76.33%, the pig raising wastewater has certain phosphorus content removal capacity, the removal rate is 35.88%, the pH value of the original pig raising wastewater can be effectively adjusted, the possibility of environmental pollution caused by fermentation waste liquid is reduced, and the resource utilization of the pig raising wastewater is better improved.
Therefore, the technology of realizing the resource utilization of the pig raising wastewater by the beneficial microorganisms has the advantages that the decontamination capability of the pig raising wastewater is not weaker than that of the domestic and foreign mainstream physicochemical treatment technology, natural treatment technology and traditional biological treatment technology, the effect is even better, and the technology has higher broad spectrum for removing pollutants in the pig raising wastewater more comprehensively; the pig raising wastewater treatment by microbial fermentation is lower in decontamination cost, less in equipment investment, short in decontamination period, free of a large amount of fields, and capable of building small fermentation equipment close to the source for pig raising wastewater treatment, and small enterprises can also bear system construction cost; the beneficial microorganisms are used for fermentation, beneficial microorganism strains can be harvested for production and use of microbial agents while the pig raising wastewater is treated, the damage of pollutants to the environment is reduced in the pig raising wastewater treatment process, economic benefits can be reported, the microbial agents can be promoted to be popularized and the development of green agriculture can be accelerated, a virtuous cycle system of ecological protection-agricultural development is created, and the method accords with the slightly large direction of sustainable development war in China.
Drawings
FIG. 1 shows the variation of biomass of WB strain in swine wastewater with time after the original and optimized carbon-nitrogen ratios;
FIG. 2 is a comparison of the change and decrease rate (A) of the total organic carbon content of the original (B) and the optimized swine wastewater (C) after treatment with WB strain;
FIG. 3 is a comparison of the change and decrease rate (A) of the total carbon content of the original (B) and the optimized swine wastewater (C) after WB strain treatment;
FIG. 4 is a comparison of the change and decrease rate (A) of the total nitrogen content of the original (B) and the optimized swine wastewater (C) after WB strain treatment;
FIG. 5 shows the change of total nitrogen, total carbon, total organic carbon and COD in the supernatant of WB strain fermentation broth;
FIG. 6 shows the change of pH and the removal rate of ammoniacal nitrogen and phosphorus in wastewater from swine production by WB strain.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.
Example 1 screening of strains and their use in treatment of wastewater from swine
1 materials and methods
1.1 sources of test materials
The strains used in the test are different kinds of pure culture beneficial microorganisms obtained by separation and screening of the subject groups, and research shows that all strains are high-efficiency strains with better industrial application potential in the aspects of plant growth promotion, biological control, pollutant control and the like, and are stored in a refrigerator at the temperature of-80 ℃ for standby application, and all strains are classified and identified by strain morphology and 16SrDNA, namely WZW03 (plantabacter sp.), LZN01 (Bacillus amyloliquefaciens), WA (Bacillus velezensis), WC (Bacillus methylophilus), WB (Bacillus velezensis), JDY (Bacillus mucolignosus) and CL01 (Sphingomonas sp). The pig raising wastewater is taken from a certain pig farm in a Fulars base region in Qiqiaohal, and the collected pig raising wastewater is stored at 4 ℃ for later experiments, wherein the basic characteristics of the pig raising wastewater are total nitrogen 403.2mg/L, total carbon 981.6mg/L, total organic carbon 361.9mg/L, phosphorus content 48.66mg/L and ammonia nitrogen 315.98mg/L, pH 8.85.85.
1.2 culture Medium
Beef extract peptone medium: 10.00g of peptone, 3.00g of beef extract, 5.00g of NaCl, 20.00g of agar, and adding no agar into a liquid culture medium, wherein the volume of distilled water is up to 1000.00 mL.
Original pig raising wastewater culture medium: pig raising wastewater transported from a pig farm.
The pig raising wastewater culture medium after the carbon-nitrogen ratio is optimized: 10g of brown sugar, and fixing the volume of the pig raising wastewater to 1000.00mL, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
1.3 high-efficiency degrading strain primary screening of pig-raising wastewater
1.3.1 seed liquid preparation
Respectively inoculating the test strains to a beef extract peptone culture medium, and culturing at 30 ℃ for 24h for activation. The activated strains are inoculated into a beef extract peptone culture medium, shaking culture is carried out at 30 ℃ and 120r/min, and then each strain cultured to the same concentration (OD 600= 1.0) is used as a seed solution for treating pig wastewater.
1.3.2 preliminary screening of strains
In order to screen strains which can grow in large quantity in the pig-raising wastewater, seed solutions of different strains are inoculated according to the inoculation amount of 1vol%, shake flask culture is carried out at 30 ℃ at 120r/min, fermentation liquor is sampled every 24h, the maximum viable bacteria amount of the strains is determined by detecting the viable bacteria number of each time period, and then strains with higher fermentation biomass are selected for subsequent experiments.
1.4 research on resource utilization effect of screened strains on pig raising wastewater
1.4.1 screening the strain to optimize the growth condition and change of treatment effect in the raw wastewater and carbon-nitrogen ratio
Because the carbon-nitrogen ratio in the raw liquid of the pig raising wastewater is not the optimum carbon-nitrogen ratio for the growth of thalli, the growth and reproduction of the raw liquid of the pig raising wastewater can be influenced, and the treatment effect of the pig raising wastewater can be reduced, the carbon-nitrogen ratio is optimized for a pig raising wastewater culture medium, and whether the content of the thalli can be increased and the treatment capacity of the pig raising wastewater can be enhanced is detected. And respectively inoculating the screened dominant strains into a beef extract peptone culture medium to prepare seed liquid, respectively inoculating the dominant strains into 300mL of original pig-raising wastewater culture medium and an optimized culture medium added with 1wt% of brown sugar according to the inoculation amount of 2vol%, carrying out shake-flask culture at 30 ℃ at 120r/min, and sampling samples every 24h to measure various indexes.
1.4.2 determination of Each index of centrifugal fermentation broth of pig-raising wastewater after optimization of carbon-nitrogen ratio by screening strains
In order to simulate whether the pig raising waste liquid treated by each strain can further reduce the potential of environmental threat in the industrial production process after obtaining the thalli, the experiment carries out index detection after centrifuging sample liquid at different fermentation times. Inoculating the screened strains into an optimized culture medium, carrying out shake flask culture at 30 ℃ and 120r/min, sampling samples every 24h, centrifuging for 5min at 12000rpm, and then carrying out index determination.
1.5 test measurement index
1.5.1 Water quality index
And (3) measuring COD, pH, total nitrogen, total carbon, total organic carbon, ammoniacal nitrogen and phosphorus content of the sample.
1.5.2 microbiological indicators
Placing 10mL of fermentation liquor into 90mL of sterile water, shaking for 30min in a shaking table at 200r/min, and then performing gradient dilution to obtain 10 -7 、10 -8 、10 -9 And (3) taking 0.1mL of the diluent, coating the diluent on a beef extract peptone culture medium plate, culturing at 30 ℃ for 24 hours, calculating the viable count (cfu/mL), and setting three groups of parallels.
2. Results and discussion
2.1 Strain screening and comparison of growth of screened strains over time in original and optimized swine wastewater media
The aim of screening different strains is achieved by detecting the maximum content of viable bacteria of each strain in the swine wastewater, and the culture of beneficial strains by using the swine wastewater discovers that WB (white cell) can grow in the swine wastewater and has higher biomass, and the maximum viable bacteria number is 2.28 multiplied by 10 10 cfu/mL。
The screened Bacillus belgii strain (Bacillus velezensis) for treating the pig-raising wastewater is named as WB, is preserved in China center for type culture Collection, and is addressed to Wuhan university, wuhan, china, and the strain preservation number is CCTCC NO: m20211547 with a preservation time of 2021, 12 and 6 days.
In order to determine the biomass of WB in different culture media, the WB was inoculated in the original culture medium and the culture medium after optimization respectively for viable count determination. As can be seen from FIG. 1, the WB strain reached a maximum biomass of 2.28X 10 after 48h in the original medium 10 cfu/mL, and the maximum biomass in brown sugar is 4.64X 10 10 cfu/mL, biomass increased 50.86%.
2.2WB growth in original and optimized pig wastewater culture Medium
2.2.1 comparison of Total organic carbon treatment in the original and optimized wastewater culture Medium for pig raising
As can be seen from fig. 2, after the WB strain is inoculated, the total organic carbon content in the original wastewater from pig raising fermentation solution increases with the increase of the culture time of the bacteria, the minimum value content of the total organic carbon after WB fermentation is 258.7mg/L compared with the control group, the total organic carbon reduction rate is 28.52%, and when WB is inoculated into the culture medium with the optimized carbon-nitrogen ratio, the total organic carbon content of the fermentation solution continuously decreases for 96 hours with the increase of the culture time to reach the minimum value, the reduction rate reaches 57.53 compared with the control group, and the consumption rate of the culture medium after optimization on the total organic carbon is faster compared with the original wastewater from pig raising culture medium.
2.2.2 comparison of the treatment of Total carbon in the original and optimized wastewater culture Medium for pig raising
And respectively inoculating WB to the original pig raising wastewater culture medium and the optimized pig raising wastewater culture medium, and performing total carbon determination on the fermentation liquor every 24 hours. As shown in FIG. 3, after the WB strain was inoculated in the original wastewater culture medium for pig raising, the total carbon content in the fermentation broth showed a tendency of slowly decreasing and then becoming stable with the increase of the culture time, the minimum value of the decrease of the total carbon content in the WB fermentation broth was 769.5mg/L, and the total carbon decrease rate of the WB fermentation broth of the screened strain was 21.61% compared with the control group. After the screened strain is inoculated to the optimized pig raising wastewater culture medium, the total carbon content in the fermentation liquor continuously decreases along with the increase of the culture time, the speed is higher, and finally the total carbon content tends to slowly decrease, the total carbon content of the fermentation liquor reaches the minimum value WB =2360mg/L after 96h of culture, and the total carbon decrease rate is reduced by 54.17 compared with a control group. And WB is cultured in the optimized culture medium, and the total carbon content reduction rate in the fermentation liquor is obviously improved compared with the total carbon reduction rate in the original pig raising wastewater.
2.2.3WB Change in Total Nitrogen in original and optimized wastewater from swine production media
And respectively inoculating WB to the original pig raising wastewater culture medium and the optimized pig raising wastewater culture medium, and measuring the total nitrogen of the fermentation liquor every 24 hours. As can be seen from FIG. 4, the total nitrogen content of the fermentation broth after the WB strain is inoculated on the original swine wastewater generally shows a decreasing trend along with the increase of the fermentation time of the strain, the total nitrogen content of the WB fermentation broth is decreased to the minimum value of 179.2mg/L when the strain is cultured for 96h, and the total nitrogen content is decreased by 55.56% compared with the control group. When the carbon-nitrogen ratio of the pig wastewater is adjusted and optimized, the WB fermentation liquor shows a general descending trend along with the prolonging of the fermentation time, the total nitrogen content rapidly descends in the fermentation interval of 0-24h and then gradually descends, the total nitrogen content reaches the minimum value of 146.6mg/L after the WB is cultured for 96h, and the total nitrogen content is reduced by 56.11% compared with that of the WB in a control group. The comparison shows that the reduction rate of the total nitrogen of the fermentation liquor of WB in the growth of the original and optimized pig raising wastewater culture media has no significant difference.
2.4 Change of Each index of fermentation waste liquid after removing thallus from the wastewater from pig raising treated by WB
The WB strain can grow and reproduce better in the optimized pig raising wastewater culture medium through comparison, so that the optimized pig raising wastewater culture medium is selected as a basic culture medium for beneficial microorganism industrial fermentation, and the change condition of pollutants in waste liquid filtered out after thalli are obtained is simulated and detected in industrial production so as to explore the degradation capability of the strain on the pollutants.
2.4.1WB changes of total nitrogen, total carbon, total organic carbon and COD content in optimized pig raising wastewater
And (4) inoculating WB into the optimized pig raising wastewater culture medium, and measuring total nitrogen, total carbon and total organic carbon of the centrifuged supernatant every 24 hours. As can be seen from FIG. 5, compared with the control group, the total nitrogen content in the supernatant of WB fermentation broth after centrifugation is reduced by 93.87%, the total carbon content is reduced by 75.53%, and the total organic carbon content is reduced by 75.06%, which indicates that the WB strain can better utilize the nutrient components in the swine wastewater to perform self growth, reproduction and metabolic activities, and prevent the filtered fermentation wastewater from causing environmental pollution due to eutrophication. In order to determine the effect of the screened strains on removing COD from the pig wastewater, COD detection is carried out on the supernatant of WB fermentation liquor, and as can be seen from FIG. 5, along with the continuous increase of the fermentation time of the strains, the COD content is continuously and rapidly reduced, and the maximum COD removal rate reaches 82%, which shows that WB has a better removal rate on COD, and the threat of the filtered waste liquor on the environment is reduced.
2.4.2WB in the optimized pig raising wastewater culture medium, the pH value changes and the removal rate of ammonia nitrogen and phosphorus
pH measurement was performed every 24h on WB fermentation broth, and it can be seen from fig. 6 that after 96h fermentation of swine wastewater by WB strain, pH was lowered from the original alkaline solution of pH =8.85 to an acidic fermentation broth of pH =5.86, probably due to secretion of organic acid during the growth of the strain. The ammonia nitrogen content of the WB strain fermentation liquid supernatant liquid in different fermentation time is measured, and as can be seen from figure 6, the WB strain can obviously reduce the ammonia nitrogen content of the fermentation waste liquid, the ammonia nitrogen removal rate reaches 76.33%, and the ammonia nitrogen removal effect is good. Phosphorus content determination of the supernatant of the fermentation liquor is carried out, and the phosphorus removal rate can reach 35.88% compared with that of WB of a control group, so that the phosphorus removal effect is achieved.
3 conclusion
(1) WB can grow in the original pig-raising wastewater, the biomass is high, the biomass is remarkably improved after the WB is inoculated by using the optimized culture medium, and the highest viable count is 4.64 multiplied by 10 10 cfu/mL。
(2) The WB strain is fermented in a pig raising wastewater culture medium with an optimized carbon-nitrogen ratio, so that COD and ammonia nitrogen can be removed well, the COD removal rate is 82%, the ammonia nitrogen removal rate is 76.33%, the pig raising wastewater has certain phosphorus content removal capacity, the removal rate is 35.88%, the pH value of the original pig raising wastewater can be effectively adjusted, the possibility of environmental pollution caused by fermentation waste liquid is reduced, and the resource utilization of the pig raising wastewater is improved well.
Claims (10)
1. A Bacillus belgii (Bacillus velezensis) strain which can be used for treating pig-raising wastewater is named as Bacillus velezensis WB and is preserved in China center for type culture Collection, and the strain preservation number is CCTCC NO: m20211547 with a preservation time of 2021, 12 and 6 days.
2. Use of the Bacillus belgii strain of claim 1 for the treatment of swine wastewater.
3. The use according to claim 2, wherein the seed solution of the Bacillus beiLeisi strain is inoculated into sterilized swine wastewater to be treated, and the swine wastewater after treatment is obtained by fermentation, and the pollutant content of the swine wastewater after treatment is reduced compared with that before treatment.
4. The use as claimed in claim 3, further comprising the step of adding brown sugar to the sterilized swine wastewater to be treated so that the swine wastewater has a carbon to nitrogen ratio of 16 to 20:1.
5. the use of claim 3, wherein the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
6. The use according to claim 3, wherein the pig wastewater after treatment has a reduced content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus compared to the pig wastewater before treatment.
7. A method for treating pig raising wastewater is characterized by comprising the following steps:
(1) Inoculating the Bacillus beilaisi strain WB of claim 1 into beef extract peptone medium for activation, and inoculating the activated strain WB into beef extract peptone mediumThe strain concentration was then adjusted to OD using sterile water 600 =1.0 as seed liquid;
(2) Inoculating the seed solution into sterilized wastewater to be treated for pig raising, and fermenting to obtain the treated wastewater for pig raising, wherein the content of pollutants in the treated wastewater for pig raising is reduced compared with that before treatment.
8. The method as claimed in claim 7, wherein the step (2) further comprises the step of adding brown sugar to the sterilized swine wastewater to be treated so that the carbon-nitrogen ratio of the swine wastewater is 16-20:1.
9. the method according to claim 7, wherein in the step (2), the seed solution is inoculated into the sterilized swine wastewater to be treated in an amount of 1 to 2 vol%; the fermentation refers to fermentation culture at 30 ℃ and 120r/min for 24-96h.
10. The method of claim 7, wherein the swine wastewater after treatment has a reduced content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus and pH compared to the swine wastewater before treatment.
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| CN117586929A (en) * | 2024-01-19 | 2024-02-23 | 南京万瑞环境科技有限公司 | Degradation strain and application thereof in cultivation wastewater treatment |
| CN117586929B (en) * | 2024-01-19 | 2024-03-26 | 南京万瑞环境科技有限公司 | Degradation strain and application thereof in cultivation wastewater treatment |
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