CN104860732A - Microbial strain organic fertilizer for improving soil alkalinity - Google Patents
Microbial strain organic fertilizer for improving soil alkalinity Download PDFInfo
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- CN104860732A CN104860732A CN201510179405.0A CN201510179405A CN104860732A CN 104860732 A CN104860732 A CN 104860732A CN 201510179405 A CN201510179405 A CN 201510179405A CN 104860732 A CN104860732 A CN 104860732A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a microbial strain organic fertilizer for improving soil alkalinity. The strain in the organic fertilizer can produce alkali under different fermentation conditions, and can be used against a soil acidification problem of over-farmed soil. Bacillus can survive and grow under stresses, can provide various enzymes for soil, and assists in improving soil microbial diversity. An alkali-producing bacillus is screened and is applied in acidic soil, and crop rhizosphere micro-ecology of the acidic soil can be tuned and optimized. Soil urease activity can be improved, such that an excellent liquid microbial fertilizer is provided for the growth of crops such as Indian lettuce and lettuce in the acidic soil. With the organic fertilizer prepared with the strain HNPY23, leaf tissue chlorophyll content, leaf Vc content and yield of the crops such as Indian lettuce and lettuce can be substantially improved. With the fertilizer, both quality and yield can be improved.
Description
Technical field
The present invention relates to microbial bacteria and organic fertilizer field, be specifically related to a kind ofly produce the rhizosphere environment that alkali can finely tune acid soil, promote microorganism organic fertilizer and the application of the growth of the crop such as Lettuce, romaine lettuce in acid soil.
Background technology
Genus bacillus is present in nature widely, have breeding fast, metabolism is fast, four hours propagation 100,000 times, standard bacteria only can breed 6 times in four hours.The key property of this genus bacterium to produce gemma unfavourable condition to special resistibility, can low temperature resistant-60 DEG C, high temperature resistant+280 DEG C without wet condition, resistance to strong acid, resistance to highly basic, antibiotic and sterilizing, resistance to hyperoxia (addicted to oxygen breeding), lower oxygen concentration resistance (anaerobism breeding).Especially many bacterium energy hydrolyzed starches of this genus, decomposing protein, pectin, alginate etc. are industrial for extracting amylase, proteolytic enzyme, polygalacturonase, dextranase, cellulase etc.They are also present in soil, plant tissue, in water and air, all closely related with nature Substance Transformation, soil fertility, environmental health.The large quadruple of genus bacillus volume ratio general pathogeny bacterium molecule, take up space advantage, suppresses the growth and breeding of harmful bacteria.They are strong to organic matter decomposition power simultaneously, while propagation, highly active amylase can be disengaged, the macromolecular substance that difficulty is decomposed is resolved into available small-molecule substance, produce abundant metabolism resultant, synthesize the materials such as multiple organic acid, enzyme, physiologically active, and other multiple nutrient be easily utilized, promote plant growth.Along with agricultural chemicals, chemical fertilizer etc. long-term, continue to use, cause two packing spaces sexual involution and the soil acidification of anthropogenic soil, genus bacillus can in adverse circumstance survival and growth, provide various enzyme for soil and increase diversity of soil microorganism.The genus bacillus that screening can produce alkali is applied in acid soil, the crop rhizosphere Tiny ecosystem of fine setting acid soil, promotes that plant growth has great importance.But the growing environment that genus bacillus is suitable for is the nutrient solution of high protein and pH value is the environment of 7 ~ 9, grow comparatively slow in acid soil, so need to carry out in advance expanding be fitted on certain number and provide enough protein environment could improve its effect.
Summary of the invention
For the problems referred to above, what the present invention aimed to provide that the high and nutrition-allocated proportion of a kind of microbial bacteria content is rationally beneficial to that bacterial classification grows fast has the microbial bacteria fertilizer improving surface soil alkalinity.
For realizing this technical purpose, the solution of the present invention is: a kind of have the microbial bacteria fertilizer improving surface soil alkalinity, comprises a kind of subtilis producing alkali;
The first step, is inoculated into subtilis in nutrient solution, carries out liquid fermenting life, working condition is, pH value is between 7.7 ~ 8.2, temperature 20 ~ 35 DEG C, and fermentation time obtains original fermented solution in 5 ~ 7 days, wherein stirring velocity is 120 ~ 300 revs/min, in described nutrient solution 1L, substances content is: peptone 3 ~ 7g, soya-bean cake 8 ~ 15g, yeast powder 1 ~ 2.5g, molasses 10 ~ 20g, KH
2pO
40.01g, sodium-chlor 0.5 ~ 1.5g, K
2hPO
40.01g; Bacterial strain HNPY23 is carried out enlarged culturing, joining original fermented solution in the product ferment liquid of new configuration according to total amount 1:4, the greenhouse being placed in 25 ~ 40 DEG C carry out enlarged culturing 3 ~ 4 days can obtain zymocyte liquid;
Second step, by the mixing microorganisms zymolyte of the pig manure become thoroughly decomposed and the dish dregs of rice in mass ratio 7: 3 ratio mix and obtain coarse fodder; Then the zymocyte liquid the first step prepared is inoculated in coarse fodder by the amount of 60 ~ 180L/ ton and carries out solid fermentation, control temperature is below 55 DEG C, ferment 6 ~ 9 days, carry out turning 1 ~ 2 times/day in fermenting process, wherein after fermentation ends, bacterial strain HNPY23 content reaches 1.1 × 10
8more than cfu/g, obtains microbial organic fertilizer;
Finally, be no more than the condition of 55 DEG C in temperature under, the water content of microbial organic fertilizer is evaporated to less than 30%, pack of finally packing.
As preferably, the mixing microorganisms zymolyte preparation method of the described dish dregs of rice is: milk-acid bacteria, yeast, genus bacillus and Geotrichum are mixed according to 5:3:4:1, mixing microorganisms is added according to 1%--2% inoculum size in the dish dregs of rice, add the water of 55 ~ 65% of dish dregs of rice quality, obtain the mixing microorganisms zymolyte of the dish dregs of rice 15 ~ 20 DEG C of temperature bottom fermentation 48 hours fermentations.
As preferably, described in the pig manure germination index that becomes thoroughly decomposed be more than 98%, quality of organic matter is than content more than 45%, and organonitrogen quality is more than 1% than content, water content mass ratio 25-30%.
The microbial bacteria of the product acid function that the present invention relates to and prepare fertilizer there is following beneficial effect:
1. the bacterial classification that this fertilizer adopts is that a kind of fermentation produces the subtilis of alkali function, all alkali can be produced under different fermentation conditions, can for the two packing spaces sexual involution of overfarming soil and soil acidification problem, genus bacillus can in adverse circumstance survival and growth, can various enzyme be provided for soil and increase diversity of soil microorganism, the genus bacillus that screening can produce alkali is applied in acid soil, can optimize the crop rhizosphere Tiny ecosystem of fine setting acid soil simultaneously.
2. carry out enlarged culturing again after subtilis being carried out elementary cultivation, the growth of bacterial strain can be accelerated, avoid bacterial strain to assemble sedimentation, also reduce simultaneously and cause growing slack-off situation because of pH value change.
3. the mixing microorganisms zymolyte of subtilis and the pig manure become thoroughly decomposed and the dish dregs of rice is mixed, effectively can solve the single strain growth of the zymocyte liquid nutrition problem slowly of single bacterial strain, bacterial strain can react with pig manure and the dish dregs of rice and improve the content of benefit materials simultaneously.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further details.
The present invention is a kind of has the microbial bacteria fertilizer improving surface soil alkalinity, comprises a kind of subtilis producing alkali;
The first step, is inoculated into subtilis in nutrient solution, carries out liquid fermenting life, working condition is, pH value is between 7.7 ~ 8.2, temperature 20 ~ 35 DEG C, and fermentation time obtains original fermented solution in 5 ~ 7 days, wherein stirring velocity is 120 ~ 300 revs/min, in described nutrient solution 1L, substances content is: peptone 3 ~ 7g, soya-bean cake 8 ~ 15g, yeast powder 1 ~ 2.5g, molasses 10 ~ 20g, KH
2pO
40.01g, sodium-chlor 0.5 ~ 1.5g, K
2hPO
40.01g; Bacterial strain HNPY23 is carried out enlarged culturing, joining original fermented solution in the product ferment liquid of new configuration according to total amount 1:4, the greenhouse being placed in 25 ~ 40 DEG C carry out enlarged culturing 3 ~ 4 days can obtain zymocyte liquid;
Second step, by the mixing microorganisms zymolyte of the pig manure become thoroughly decomposed and the dish dregs of rice in mass ratio 7: 3 ratio mix and obtain coarse fodder; Then the zymocyte liquid the first step prepared is inoculated in coarse fodder by the amount of 60 ~ 180L/ ton and carries out solid fermentation, control temperature is below 55 DEG C, ferment 6 ~ 9 days, carry out turning 1 ~ 2 times/day in fermenting process, wherein after fermentation ends, bacterial strain HNPY23 content reaches 1.1 × 10
8more than cfu/g, obtains microbial organic fertilizer;
Finally, be no more than the condition of 55 DEG C in temperature under, the water content of microbial organic fertilizer is evaporated to less than 30%, pack of finally packing.
As preferably, the mixing microorganisms zymolyte preparation method of the described dish dregs of rice is: milk-acid bacteria, yeast, genus bacillus and Geotrichum are mixed according to 5:3:4:1, mixing microorganisms is added according to 1%--2% inoculum size in the dish dregs of rice, add the water of 55 ~ 65% of dish dregs of rice quality, obtain the mixing microorganisms zymolyte of the dish dregs of rice 15 ~ 20 DEG C of temperature bottom fermentation 48 hours fermentations.
As preferably, described in the pig manure germination index that becomes thoroughly decomposed be more than 98%, quality of organic matter is than content more than 45%, and organonitrogen quality is more than 1% than content, water content mass ratio 25-30%.
Three, potted plant experiment:
(1) romaine lettuce potted plant experiment
1.1 use: mixing with soil uniformity, and original initial pH value is 5.0, and pot experiment does 4 process, and 5 repetitions, process as follows by substratum 1 tunning.I1: chemical fertilizer; I2: aseptic culture fluid; I3: microbial organic fertilizer; CK: only water clear water and do not apply fertilizer.Plant size, romaine lettuce seedling that growing way is close in above four process, every basin 1 seedling, other daily administrations are consistent.
1.2 results:
After surrounding, the pH value of the soil of I3 group is raised to 8.2 from 5.
I3 is other contrast lifting capacitys (%) organized relatively:
| I1 | I2 | CK | |
| Soil urease activity | 25.12 | 28.96 | 40.50 |
| Chlorophyll content | / | / | 37.06 |
| Vc content | 37.54 | 39.43 | 65.43 |
| Output | 17.12 | 26..4 | 38.51 |
As can be seen from above-mentioned data, high by the romaine lettuce output of the fertilizer plantation containing bacterial strain HNPY23, the content of Vc content and soil urease activity only waters clear water apparently higher than I1 chemical fertilizer group, I2 equivalent blank nutritive medium group and CK and not to apply fertilizer group, and its Determination of Chlorophyll only waters clear water group of not applying fertilizer relative to CK obvious lifting.
(2) Lettuce potted plant experiment
2.1 use: mixing with soil uniformity, and original initial pH value is 5.0, and pot experiment does 4 process, and 5 repetitions, process as follows by substratum 1 tunning.I1: chemical fertilizer; I2: aseptic culture fluid; I3: microbial organic fertilizer; CK: only water clear water and do not apply fertilizer.Plant size, Lettuce seedling that growing way is close in above four process, every basin 3 seedlings, other daily administrations are consistent.
2.3 results:
After surrounding, the pH value of the soil of I3 group is raised to 8.4 from 5.
I3 is other contrast lifting capacitys (%) organized relatively:
| I1 | I2 | CK | |
| Vc content | 25.31 | 23.27 | 34.56 |
| Output | 19.16 | 26.41 | 37.37 |
As can be seen from above-mentioned data, the romaine lettuce output of planting with fertilizer containing bacterial strain HNPY23 is high, and the content of Vc content and output only waters clear water apparently higher than I1 chemical fertilizer group, I2 equivalent blank nutritive medium group and CK and not apply fertilizer group.
The above; be only preferred embodiment of the present invention; not in order to limit the present invention, every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalently replace and improve, within the protection domain that all should be included in technical solution of the present invention.
Claims (3)
1. there is the microbial bacteria fertilizer improving surface soil alkalinity, it is characterized in that: comprise a kind of subtilis producing alkali;
The first step, is inoculated into subtilis in nutrient solution, carries out liquid fermenting life, working condition is, pH value is between 7.7 ~ 8.2, temperature 20 ~ 35 DEG C, and fermentation time obtains original fermented solution in 5 ~ 7 days, wherein stirring velocity is 120 ~ 300 revs/min, in described nutrient solution 1L, substances content is: peptone 3 ~ 7g, soya-bean cake 8 ~ 15g, yeast powder 1 ~ 2.5g, molasses 10 ~ 20g, KH
2pO
40.01g, sodium-chlor 0.5 ~ 1.5g, K
2hPO
40.01g; Bacterial strain HNPY23 is carried out enlarged culturing, joining original fermented solution in the product ferment liquid of new configuration according to total amount 1:4, the greenhouse being placed in 25 ~ 40 DEG C carry out enlarged culturing 3 ~ 4 days can obtain zymocyte liquid;
Second step, by the mixing microorganisms zymolyte of the pig manure become thoroughly decomposed and the dish dregs of rice in mass ratio 7: 3 ratio mix and obtain coarse fodder; Then the zymocyte liquid the first step prepared is inoculated in coarse fodder by the amount of 60 ~ 180L/ ton and carries out solid fermentation, control temperature is below 55 DEG C, ferment 6 ~ 9 days, carry out turning 1 ~ 2 times/day in fermenting process, wherein after fermentation ends, bacterial strain HNPY23 content reaches 1.1 × 10
8more than cfu/g, obtains microbial organic fertilizer;
Finally, be no more than the condition of 55 DEG C in temperature under, the water content of microbial organic fertilizer is evaporated to less than 30%, pack of finally packing.
2. having described in 1 to be gone to improve the microbial bacteria fertilizer of soil acidity according to right, it is characterized in that: the mixing microorganisms zymolyte preparation method of the described dish dregs of rice is: milk-acid bacteria, yeast, genus bacillus and Geotrichum are mixed according to 5:3:4:1, mixing microorganisms is added according to 1%--2% inoculum size in the dish dregs of rice, add the water of 55 ~ 65% of dish dregs of rice quality, obtain the mixing microorganisms zymolyte of the dish dregs of rice 15 ~ 20 DEG C of temperature bottom fermentation 48 hours fermentations.
3. having described in 1 to be gone to improve the microbial bacteria fertilizer of soil acidity according to right, it is characterized in that: described in the pig manure germination index that becomes thoroughly decomposed be more than 98%, quality of organic matter is than content more than 45%, and organonitrogen quality is more than 1% than content, water content mass ratio 25-30%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510179405.0A CN104860732A (en) | 2015-04-14 | 2015-04-14 | Microbial strain organic fertilizer for improving soil alkalinity |
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| CN201510179405.0A CN104860732A (en) | 2015-04-14 | 2015-04-14 | Microbial strain organic fertilizer for improving soil alkalinity |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105295944A (en) * | 2015-11-24 | 2016-02-03 | 赵洪军 | Biological soil conditioner with acidic soil improvement function |
| CN107266261A (en) * | 2017-08-06 | 2017-10-20 | 长沙小新新能源科技有限公司 | A kind of composite microbe fertilizer |
| CN107445778A (en) * | 2017-10-10 | 2017-12-08 | 南京仙草堂生物科技有限公司 | A kind of preparation method and application process of the biological organic fertilizer for improving soil organic fractions |
| CN110872518A (en) * | 2019-11-20 | 2020-03-10 | 广西金穗生态科技股份有限公司 | Acid soil conditioner and preparation method and application thereof |
| CN112159287A (en) * | 2020-10-14 | 2021-01-01 | 中南林业科技大学 | High-efficacy foliar fertilizer for eucalyptus and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5610029B1 (en) * | 1970-06-29 | 1981-03-05 | ||
| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
| CN102690151A (en) * | 2012-06-28 | 2012-09-26 | 赵鹏 | Composite microbial inoculum organic multielement compound fertilizer and production method thereof |
| CN103320137A (en) * | 2013-04-03 | 2013-09-25 | 清华大学 | Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof |
| CN103333832A (en) * | 2013-07-01 | 2013-10-02 | 南京大学 | Apophysomycesspartina as well as acid-producing fermentation method and application thereof |
-
2015
- 2015-04-14 CN CN201510179405.0A patent/CN104860732A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5610029B1 (en) * | 1970-06-29 | 1981-03-05 | ||
| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
| CN102690151A (en) * | 2012-06-28 | 2012-09-26 | 赵鹏 | Composite microbial inoculum organic multielement compound fertilizer and production method thereof |
| CN103320137A (en) * | 2013-04-03 | 2013-09-25 | 清华大学 | Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof |
| CN103333832A (en) * | 2013-07-01 | 2013-10-02 | 南京大学 | Apophysomycesspartina as well as acid-producing fermentation method and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105295944A (en) * | 2015-11-24 | 2016-02-03 | 赵洪军 | Biological soil conditioner with acidic soil improvement function |
| CN107266261A (en) * | 2017-08-06 | 2017-10-20 | 长沙小新新能源科技有限公司 | A kind of composite microbe fertilizer |
| CN107445778A (en) * | 2017-10-10 | 2017-12-08 | 南京仙草堂生物科技有限公司 | A kind of preparation method and application process of the biological organic fertilizer for improving soil organic fractions |
| CN110872518A (en) * | 2019-11-20 | 2020-03-10 | 广西金穗生态科技股份有限公司 | Acid soil conditioner and preparation method and application thereof |
| CN110872518B (en) * | 2019-11-20 | 2021-10-08 | 广西金穗生态科技集团股份有限公司 | Acid soil conditioner and preparation method and application thereof |
| CN112159287A (en) * | 2020-10-14 | 2021-01-01 | 中南林业科技大学 | High-efficacy foliar fertilizer for eucalyptus and preparation method thereof |
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Application publication date: 20150826 |