CN109197206A - A method of shortening mango breeding cycle - Google Patents

A method of shortening mango breeding cycle Download PDF

Info

Publication number
CN109197206A
CN109197206A CN201811235376.5A CN201811235376A CN109197206A CN 109197206 A CN109197206 A CN 109197206A CN 201811235376 A CN201811235376 A CN 201811235376A CN 109197206 A CN109197206 A CN 109197206A
Authority
CN
China
Prior art keywords
mango
parts
branch
awns
seasons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201811235376.5A
Other languages
Chinese (zh)
Other versions
CN109197206B (en
Inventor
苏伟强
邱文武
刘业强
王小媚
蔡昭艳
李恒锐
董龙
方位宽
任惠
黄章保
郭凌飞
卢美瑛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baise Mango Research Institute
Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Original Assignee
Horticultural Research Institute of Guangxi Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Horticultural Research Institute of Guangxi Academy of Agricultural Sciences filed Critical Horticultural Research Institute of Guangxi Academy of Agricultural Sciences
Priority to CN201811235376.5A priority Critical patent/CN109197206B/en
Publication of CN109197206A publication Critical patent/CN109197206A/en
Application granted granted Critical
Publication of CN109197206B publication Critical patent/CN109197206B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention discloses a kind of method for shortening mango breeding cycle, belongs to fruit breeding technical field.The present invention selects four seasons honey awns as stock tree, graft hybridization parent, obtain hybrid seed, then it begins sowing in good time and cultivates hybrid seed seedling, using the aging healthy and strong terminal bud of 1 tip of seedling extraction as scion, grafting is in the bearing basal shoot top for the four seasons awns bearing tree that will be bloomed, the florigen for having accumulated and having been formed using its fruit tree of growing up, induction is still in the scion branch of juvenile phase, and grafting twig can yield positive results for 3-5 months after grafting.The present invention carries out passing colored pollination using calliphorid, and rate of yielding positive results is 90% or more.Method of the invention is simple and easy, only needs 10 months or so from seeding and seedling raising to yielding positive results, can preliminary assessment select filial generation, new varieties can be selected in 2-3, substantially reduce the shortening mango crossbreeding time limit.

Description

A method of shortening mango breeding cycle
Technical field
The invention belongs to fruit breeding technical fields, and in particular to a method of shorten mango breeding cycle.
Background technique
Mango is tropical fruit (tree) famous in the world, usually enjoys the good reputation of " king of tropical fruit (tree) ", the deep happiness by people Love.Mango main producing region one of of the Guangxi because of advantageous geographical conditions and as the country, the Gui Qimang, Tai Nongmang produced, Jin Huangmang, pale reddish brown awns etc. are well-known throughout the country, are exported to all parts of the country.
The quality of fruit sales volume, a big chunk reason are the quality of fruit variety, and mango is no exception, therefore, training It educates and finds that mango new varieties play an important role the development of mango planting industry.To obtain high yield, mango is carried out Breed improvement is imperative.Since mango is the heterozygote of height, sexual hybridization is mainly carried out between kind, but by various The phenomenon that factor influences, and often the florescence occurs between each kind and does not meet, in addition, mango pollen is small, along with traditional artificial miscellaneous Friendship technology is immature, therefore the crossbreeding Comparision of mango is difficult, and effect is very low.
Due to undergo a juvenile phase stage in mango seedling tree growth and development process, if not right early period in mango seedling tree It is handled, and generally requiring could fruiting by 5-7.As it can be seen that being grown to mango seedling tree again etc. by mango hybrid seed It identifies that the selection of true and false hybrid undoubtedly takes a substantial amount of time and manpower, material resources and financial resources to its fruiting, influences mango hybridization The process of breeding.Grafting is able to maintain the hereditary capacity of scion, has a wide range of applications in the crossbreeding of fruit tree, traditional transfers The mode of connecing is to be carried out using longer scion, but this for the fruiting period for shortening mango, effect is unobvious.
Therefore, low for the length of breeding cycle existing for traditional grafting method and the pollination efficiency of mango artificial hybridization at this stage Under, the problems such as setting percentage is low for pollination, labour cost is high and long period, it is necessary to explore a kind of shortening mango breeding cycle Method, accelerate mango crossbreeding process.
Summary of the invention
It is an object of the present invention to provide a kind of methods for shortening mango breeding cycle, by the way that different mango Hybrids is real The result that the aging healthy and strong terminal bud of 1 tip of raw seedling extraction is grafted onto the four seasons honey Mang Shu that same will bloom as scion is female On branch, yields positive results within 3-5 months after grafting not only may be implemented, can also realize and several times a year yield positive results, and yield positive results Rate is high, realizes the purpose for accelerating the process of mango crossbreeding.
The invention is realized by the following technical scheme:
A method of shortening mango breeding cycle, comprising the following steps:
(1) acquisition of filial generation seed:
1. choosing the raw four seasons awns of 5-8 as stock tree, two parental breed intervals for being intended to hybridization are grafted four On the three-level major branch of Ji Mang, 3-6 tree is grafted in each cross combination;Brandreth is built in four seasons awns surrounding with steel pipe, in grafted branch The heading stage of item to small fruiting period takes 32-40 mesh fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is carried out in the flower spike pumping 4-6cm long of grafting twig Pollination after pollination, carries out Routine Management, to obtain the mango hybrid seed of directive breeding;
(2) cultivation of seedling: sterile-processed mango hybrid seed is seeded into 7-9 month and fills clean river sand Hole tray in, cross seed 1-2cm with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long extremely Field planting is transplanted to when 8-10cm;
(3) stock selects: choosing four seasons awns that 5-8 gives birth to as parent, selects the branch of robust growth as a result Female branch, every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 3-6 tree, and each tree grafts 30- 50 branches;Brandreth is built in four seasons awns surrounding with steel pipe, takes 32-40 mesh insect prevention in heading stage to the small fruiting period of grafting twig Net;
As a preferred option of the technical scheme, the diameter of the bearing basal shoot is 4-6mm.
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the 10-11 month of current year, transferred with point of a knife from female branch needs The position connect is chosen out by bast from xylem;
2. mango hybridization seedling is cut from terminal bud 3-4cm, obtained tender tip is as scion, by scion main bud base portion It is whittled into the long bevel that a length is 1-1.5cm on one side, then opposite is whittled into the short bevel that length is 0.5-0.7cm, makes scion Lower end is at flat wedge;10-15min is impregnated into the scion cut into the water, is placed into mixed containing methyl α-naphthyl acetate and heteroauxin It closes in solution and impregnates 3-5min, dried after taking-up;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel is no longer outer It is exposed to female branch section;After scion is plugged, the film for being 1-2cm with width will bind tight at graft union;
4. spraying nutrient solution to stock branch after having grafted, sprayed every 7-10 days 1 time, until graft union organization healing, Realize normal growth;Insect prevention microbial inoculum is sprayed to whole strain four seasons awns simultaneously;
As a preferred option of the technical scheme, the terminal bud is the aging healthy and strong terminal bud for 1 tip that mango hybridizes seedling extraction.
As a preferred option of the technical scheme, the mass ratio of methyl α-naphthyl acetate and heteroauxin is 1-3:1 in the mixed solution, described Mixed solution is that the methyl α-naphthyl acetate of 10-30mg and heteroauxin are dissolved in obtained in 1L 80-95% ethanol solution.
As a preferred option of the technical scheme, the nutrient solution be 5-7 parts of ammonium phosphate, 5-7 parts of potassium nitrate, 3-5 parts of ammonium sulfate, 0.8-1.5 parts of calcium chloride, 0.5-0.8 parts of magnesium sulfate and 0.3-0.5 parts of boric acid are dissolved in obtained in 500-800 parts of water.
As a preferred option of the technical scheme, the insect prevention microbial inoculum is that 5-7 parts of carbendazol wettable powders, 3-5 parts of Te Fuling can Wet powder and 0.5-1 parts of pesticide synergistic agents are dissolved in obtained in 500-700 parts of water.
As a preferred option of the technical scheme, 30-40 days tissues of the film bound at the graft union after grafting are cured completely It dismantles after conjunction.
(5) management after grafting, comprising:
1. fertilising: being 20-30cm digging width at mango trunk 50-60cm, depth is the annular ditch of 30-40cm, is put into The mixed fertilizer of urea, potassium chloride, calcium superphosphate, well-rotted farmyard manure composition, then the soil of one layer of 8-10cm is covered, depending on fruit tree growth feelings Condition is every putting disposable fertilizer the 2-3 month, while spraying concentration is the potassium dihydrogen phosphate of 0.2-0.4% to blade face, continuously sprays 2-3 It is secondary, every minor tick 10-12 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;It is anti-with conventional method Control mango pest and disease damage;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net It pollinates in canopy, after pollination, carries out Routine Management.
As a preferred option of the technical scheme, the mixed fertilizer is by 10-15 parts of urea, 7-12 parts of potassium chloride, 5-7 parts of peroxophosphoric acid Calcium, 10-15 parts of well-rotted farmyard manure compositions.
The parental breed of planning crossing of the invention is Jin Huangmang, Tai Nongmang, pale reddish brown awns and Gui Qimang.
Calliphorid of the invention is used containing 80% soybean powder and 20% fish meal captive breeding.
It is above-mentioned methyl α-naphthyl acetate, heteroauxin, ammonium phosphate, potassium nitrate, ammonium sulfate, calcium chloride, magnesium sulfate, boric acid, more Bacterium spirit wettable powder, Te Fuling wettable powder, pesticide synergistic agent, urea, potassium chloride, calcium superphosphate, well-rotted farmyard manure, phosphorus Acid dihydride potassium can be commercially available from the market, be not required to especially prepare, and the medical fluid that the percentage mentioned refers both to after dilution is dense Degree.
Compared with prior art, it advantages of the present invention and has the beneficial effect that
(1) 10-11 of the aging healthy and strong terminal bud for 1 tip that method of the invention extracts mango hybridization seedling out in current year Carry out bud grafting month, grafting twig can yield positive results through 3-5 months, and rate of yielding positive results is 90% or more, is greatly shortened Mango is yielded positive results the period.
(2) present invention carries out scion breeding using the aging healthy and strong terminal bud of 1 tip of mango hybridization seedling extraction, not only The character of seedling can be observed, and since the degree of lignification of terminal bud is low, histocyte is active, and graft union can faster heal, Survival rate is higher.
(3) present invention assists mango flower pollination hybridization using the calliphorid more than hair itself, in calliphorid flight course, compared to than Other common flies can be stained with more pollen, can significantly improve the efficiency of mango flower pollination and the setting percentage of pollination.And this Invention is will to launch in network access canopy near the calliphorid larva for the phase of pupating, and when mango enters full-bloom stage, calliphorid has also just hatched At realization efficient operation, this can not only shorten pollination time, and greatly improve pollination efficiency.Under double selection, mentioning Cost of labor is saved while the setting percentage for the efficiency and pollination that high mango flower is pollinated, overcomes current manual's hybridization pollination efficiency Lowly, the problem of pollination setting percentage is low, labour cost is high and long period.
(4) method of the invention is simple and easy, only needs 10 months or so from seeding and seedling raising to yielding positive results, can be preliminary Filial generation is selected in evaluation;And four seasons awns perpetual bloom, it may be implemented several times a year to yield positive results, therefore using of the invention Method can select mango new varieties in 2-3, and more existing mango breeding time at least shortens 2-3, significantly It shortens and shortens the mango crossbreeding time limit.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is described in more detail, but is not limited to protection scope of the present invention.
Embodiment 1
A method of shortening mango breeding cycle, comprising the following steps:
(1) acquisition of filial generation seed:
1. choosing 6 years raw four seasons awns as stock tree, the bright awns of gold and the interval Tai Nongmang are grafted to the three-level in four seasons awns On major branch, 5 trees are grafted;Brandreth is built in four seasons awns surrounding with steel pipe, takes 36 mesh in heading stage to the small fruiting period of grafting twig Fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is carried out in the flower spike pumping 4-6cm long of grafting twig Pollination after pollination, carries out Routine Management, to obtain the mango hybrid seed of directive breeding;
(2) cultivation of seedling: sterile-processed mango hybrid seed is seeded into August part and fills clean river sand In hole tray, seed 1-2cm is crossed with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long to 8- Field planting is transplanted to when 10cm;
(3) stock selects: choosing 6 years raw four seasons awns as parent, selects the diameter of robust growth for 4-6mm branch Female branch as a result, every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 5 trees, each tree Graft 40 branches;Brandreth is built in four seasons awns surrounding with steel pipe, takes 36 mesh insect prevention in heading stage to the small fruiting period of grafting twig Net;
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the October of current year, need to graft from female branch with point of a knife Position is chosen out by bast from xylem;
2. by being cut at the aging healthy and strong terminal bud 3-4cm of 1 tip of mango hybridization seedling extraction, obtained tender tip conduct Scion main bud base portion is whittled into the long bevel that a length is 1.2-1.5cm by scion on one side, then it is 0.5- that opposite, which is whittled into length, The short bevel of 0.7cm, makes scion lower end at flat wedge;12min is impregnated into the scion cut into the water, places into the naphthalene of 20mg Acetic acid and 10mg heteroauxin, which are dissolved in the mixed solution that 90% ethanol solution of 1L obtains, impregnates 4min, dries after taking-up;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel is no longer outer It is exposed to female branch section;After scion is plugged, the film for being 1.5cm with width will bind tight 35 days groups after grafting at graft union It dismantles after casting off full healing;
4. spraying 6 parts of ammonium phosphate, 6 parts of potassium nitrate, 4 parts of ammonium sulfate, 1.2 parts of calcium chloride, 0.7 to stock branch after having grafted Part magnesium sulfate and 0.4 part of boric acid are dissolved in nutrient solution obtained in 700 parts of water, spray every 8 days 1 time, until graft union tissue is cured It closes, realizes normal growth;Simultaneously to whole strain four seasons awns spray 6 parts of carbendazol wettable powders, 4 parts of Te Fuling wettable powders and 0.8 part of pesticide synergistic agent is dissolved in insect prevention microbial inoculum obtained in 600 parts of water;
(5) management after grafting, comprising:
1. fertilising: apart from mango trunk 55cm at dig width be 25cm, depth for 35cm annular ditch, be put into 12 parts of urea, The mixed fertilizer that 10 parts of potassium chloride, 6 parts of calcium superphosphate, 12 parts of well-rotted farmyard manures form, then the soil of one layer of 8-10cm is covered, depending on fruit tree Growing state is every putting disposable fertilizer the 2-3 month, while the potassium dihydrogen phosphate that spraying concentration is 0.3% continuously sprays 3 to blade face It is secondary, every minor tick 10 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;It is anti-with conventional method Control mango pest and disease damage;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net It pollinates in canopy, after pollination, carries out Routine Management.
It is 3 months from the time for being grafted onto result, and has 94.4% to yield positive results in the branch grafted.By training in 2 years It educates, has filtered out the hybrid new breed of Jin Huangmang and Tai Nongmang.
Embodiment 2
A method of shortening mango breeding cycle, comprising the following steps:
(1) acquisition of filial generation seed:
1. choosing 6 years raw four seasons awns as stock tree, golden bright awns and pale reddish brown awns interval are grafted to the three-level in four seasons awns On major branch, 5 trees are grafted;Brandreth is built in four seasons awns surrounding with steel pipe, takes 35 mesh in heading stage to the small fruiting period of grafting twig Fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is carried out in the flower spike pumping 4-6cm long of grafting twig Pollination after pollination, carries out Routine Management, to obtain the mango hybrid seed of directive breeding;
(2) cultivation of seedling: sterile-processed mango hybrid seed is seeded into August part and fills clean river sand In hole tray, seed 1-2cm is crossed with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long to 8- Field planting is transplanted to when 10cm;
(3) stock selects: choosing 6 years raw four seasons awns as parent, selects the diameter of robust growth for 4-6mm branch Female branch as a result, every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 5 trees, each tree Graft 40 branches;Brandreth is built in four seasons awns surrounding with steel pipe, takes 35 mesh insect prevention in heading stage to the small fruiting period of grafting twig Net;
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the October of current year, need to graft from female branch with point of a knife Position is chosen out by bast from xylem;
2. by being cut at the aging healthy and strong terminal bud 3-4cm of 1 tip of mango hybridization seedling extraction, obtained tender tip conduct Scion main bud base portion is whittled into the long bevel that a length is 1-1.2cm by scion on one side, then it is 0.5- that opposite, which is whittled into length, The short bevel of 0.7cm, makes scion lower end at flat wedge;13min is impregnated into the scion cut into the water, places into the naphthalene of 18mg Acetic acid and 12mg heteroauxin, which are dissolved in the mixed solution that 90% ethanol solution of 1L obtains, impregnates 4min, dries after taking-up;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel is no longer outer It is exposed to female branch section;After scion is plugged, the film for being 1.2cm with width will bind tight 35 days groups after grafting at graft union It dismantles after casting off full healing;
4. spraying 6.5 parts of ammonium phosphate, 5.5 parts of potassium nitrate, 4.5 parts of ammonium sulfate, 1 part of chlorination to stock branch after having grafted Calcium, 0.6 part of magnesium sulfate and 0.4 part of boric acid are dissolved in nutrient solution obtained in 650 parts of water, spray every 9 days 1 time, until graft union Normal growth is realized in organization healing;Spraying 6.5 parts of carbendazol wettable powders, 3.5 parts of Te Fuling to whole strain four seasons awns simultaneously can Wet powder and 0.9 part of pesticide synergistic agent are dissolved in insect prevention microbial inoculum obtained in 650 parts of water;
(5) management after grafting, comprising:
1. fertilising: apart from mango trunk 55cm at dig width be 25cm, depth for 35cm annular ditch, be put into 13 parts of urea, The mixed fertilizer that 11 parts of potassium chloride, 6.5 parts of calcium superphosphate, 14 parts of well-rotted farmyard manures form, then the soil of one layer of 8-10cm is covered, depending on fruit Tree growing state every putting disposable fertilizer 2-3 month, while spraying concentration for 0.3% potassium dihydrogen phosphate to blade face, continuously spray 2 It is secondary, every minor tick 10 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;It is anti-with conventional method Control mango pest and disease damage;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net It pollinates in canopy, after pollination, carries out Routine Management.
It is 3 months from the time for being grafted onto result, and has 93.6% to yield positive results in the branch grafted.By training in 2 years It educates, has filtered out the hybrid new breed of Jin Huangmang and pale reddish brown awns.
Embodiment 3
A method of shortening mango breeding cycle, comprising the following steps:
(1) acquisition of filial generation seed:
1. choosing 7 years raw four seasons awns as stock tree, Tai Nongmang and pale reddish brown awns interval are grafted to the three-level in four seasons awns On major branch, 4 trees are grafted;Brandreth is built in four seasons awns surrounding with steel pipe, takes 34 mesh in heading stage to the small fruiting period of grafting twig Fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is carried out in the flower spike pumping 4-6cm long of grafting twig Pollination after pollination, carries out Routine Management, to obtain the mango hybrid seed of directive breeding;
(2) cultivation of seedling: sterile-processed mango hybrid seed is seeded into July and fills clean river sand In hole tray, seed 1-2cm is crossed with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long to 8- Field planting is transplanted to when 10cm;
(3) stock selects: choosing 7 years raw four seasons awns as parent, selects the diameter of robust growth for 4-6mm branch Female branch as a result, every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 4 trees, each tree Graft 45 branches;Brandreth is built in four seasons awns surrounding with steel pipe, takes 34 mesh insect prevention in heading stage to the small fruiting period of grafting twig Net;
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the November of current year, need to graft from female branch with point of a knife Position is chosen out by bast from xylem;
2. by being cut at the aging healthy and strong terminal bud 3-4cm of 1 tip of mango hybridization seedling extraction, obtained tender tip conduct Scion main bud base portion is whittled into the long bevel that a length is 1-1.3cm by scion on one side, then it is 0.5- that opposite, which is whittled into length, The short bevel of 0.7cm, makes scion lower end at flat wedge;14min is impregnated into the scion cut into the water, places into the naphthalene of 15mg Acetic acid and 15mg heteroauxin, which are dissolved in the mixed solution that 85% ethanol solution of 1L obtains, impregnates 5min, dries after taking-up;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel is no longer outer It is exposed to female branch section;After scion is plugged, the film for being 1.8cm with width will bind tight 32 days groups after grafting at graft union It dismantles after casting off full healing;
4. spraying 6 parts of ammonium phosphate, 6.5 parts of potassium nitrate, 3.5 parts of ammonium sulfate, 1.3 parts of chlorinations to stock branch after having grafted Calcium, 0.7 part of magnesium sulfate and 0.5 part of boric acid are dissolved in nutrient solution obtained in 750 parts of water, spray every 8 days 1 time, until graft union Normal growth is realized in organization healing;Spraying 5.5 parts of carbendazol wettable powders, 4.5 parts of Te Fuling to whole strain four seasons awns simultaneously can Wet powder and 0.7 part of pesticide synergistic agent are dissolved in insect prevention microbial inoculum obtained in 550 parts of water;
(5) management after grafting, comprising:
1. fertilising: being 30cm digging width at mango trunk 50cm, depth is the annular ditch of 40cm, is put into 14 parts of urea, 9 The mixed fertilizer that part potassium chloride, 5.5 parts of calcium superphosphate, 13 parts of well-rotted farmyard manures form, then the soil of one layer of 8-10cm is covered, depending on fruit tree Growing state is every putting disposable fertilizer the 2-3 month, while the potassium dihydrogen phosphate that spraying concentration is 0.4% continuously sprays 3 to blade face It is secondary, every minor tick 12 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;It is anti-with conventional method Control mango pest and disease damage;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net It pollinates in canopy, after pollination, carries out Routine Management.
It is 4 months from the time for being grafted onto result, and has 93.2% to yield positive results in the branch grafted.By training in 2 years It educates, has filtered out the hybrid new breed of Tai Nongmang and pale reddish brown awns.
Embodiment 4
A method of shortening mango breeding cycle, comprising the following steps:
(1) acquisition of filial generation seed:
1. choosing 7 years raw four seasons awns as stock tree, the bright awns of gold and seven awns interval of osmanthus are grafted to the three-level in four seasons awns On major branch, 4 trees are grafted;Brandreth is built in four seasons awns surrounding with steel pipe, takes 38 mesh in heading stage to the small fruiting period of grafting twig Fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is carried out in the flower spike pumping 4-6cm long of grafting twig Pollination after pollination, carries out Routine Management, to obtain the mango hybrid seed of directive breeding;
(2) cultivation of seedling: sterile-processed mango hybrid seed is seeded into September part and fills clean river sand In hole tray, seed 1-2cm is crossed with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long to 8- Field planting is transplanted to when 10cm;
(3) stock selects: choosing 7 years raw four seasons awns as parent, selects the diameter of robust growth for 4-6mm branch Female branch as a result, every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 4 trees, each tree Graft 35 branches;Brandreth is built in four seasons awns surrounding with steel pipe, takes 38 mesh insect prevention in heading stage to the small fruiting period of grafting twig Net;
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the November of current year, need to graft from female branch with point of a knife Position is chosen out by bast from xylem;
2. by being cut at the aging healthy and strong terminal bud 3-4cm of 1 tip of mango hybridization seedling extraction, obtained tender tip conduct Scion main bud base portion is whittled into the long bevel that a length is 1.3-1.5cm by scion on one side, then it is 0.5- that opposite, which is whittled into length, The short bevel of 0.7cm, makes scion lower end at flat wedge;11min is impregnated into the scion cut into the water, places into 22.5mg's Methyl α-naphthyl acetate and 7.5mg heteroauxin, which are dissolved in the mixed solution that 85% ethanol solution of 1L obtains, impregnates 3min, dries in the air after taking-up It is dry;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel is no longer outer It is exposed to female branch section;After scion is plugged, the film for being 2cm with width will bind tight, 38 days tissues after grafting at graft union It dismantles after healing completely;
4. spraying 5.5 parts of ammonium phosphate, 6 parts of potassium nitrate, 4 parts of ammonium sulfate, 1 part of calcium chloride, 0.7 to stock branch after having grafted Part magnesium sulfate and 0.5 part of boric acid are dissolved in nutrient solution obtained in 600 parts of water, spray every 9 days 1 time, until graft union tissue is cured It closes, realizes normal growth;6 parts of carbendazol wettable powders, 3.5 parts of Te Fuling wettable powders are sprayed to whole strain four seasons awns simultaneously Insect prevention microbial inoculum obtained in 600 parts of water is dissolved in 0.6 part of pesticide synergistic agent;
(5) management after grafting, comprising:
1. fertilising: being 25cm digging width at mango trunk 60cm, depth is the annular ditch of 40cm, is put into 11 parts of urea, 8 The mixed fertilizer that part potassium chloride, 6 parts of calcium superphosphate, 11 parts of well-rotted farmyard manures form, then the soil of one layer of 8-10cm is covered, it is raw depending on fruit tree Long situation is every putting disposable fertilizer the 2-3 month, while the potassium dihydrogen phosphate that spraying concentration is 0.3% is to blade face, continuous spray 3 times, Every minor tick 11 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;It is anti-with conventional method Control mango pest and disease damage;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net It pollinates in canopy, after pollination, carries out Routine Management.
It is 4 months from the time for being grafted onto result, and has 92.8% to yield positive results in the branch grafted.By training in 3 years It educates, has filtered out the hybrid new breed of Jin Huangmang and Gui Qimang.
Embodiment 5
A method of shortening mango breeding cycle, comprising the following steps:
(1) acquisition of filial generation seed:
1. choosing 5 years raw four seasons awns as stock tree, Tai Nongmang and seven awns interval of osmanthus are grafted to the three-level in four seasons awns On major branch, 6 trees are grafted;Brandreth is built in four seasons awns surrounding with steel pipe, takes 32 mesh in heading stage to the small fruiting period of grafting twig Fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is carried out in the flower spike pumping 4-6cm long of grafting twig Pollination after pollination, carries out Routine Management, to obtain the mango hybrid seed of directive breeding;
(2) cultivation of seedling: sterile-processed mango hybrid seed is seeded into September part and fills clean river sand In hole tray, seed 1-2cm is crossed with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long to 8- Field planting is transplanted to when 10cm;
(3) stock selects: choosing 5 years raw four seasons awns as parent, selects the diameter of robust growth for 4-6mm branch Female branch as a result, every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 6 trees, each tree Graft 50 branches;Brandreth is built in four seasons awns surrounding with steel pipe, takes 32 mesh insect prevention in heading stage to the small fruiting period of grafting twig Net;
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the October of current year, need to graft from female branch with point of a knife Position is chosen out by bast from xylem;
2. by being cut at the aging healthy and strong terminal bud 3-4cm of 1 tip of mango hybridization seedling extraction, obtained tender tip conduct Scion main bud base portion is whittled into the long bevel that a length is 1-1.5cm by scion on one side, then it is 0.5- that opposite, which is whittled into length, The short bevel of 0.7cm, makes scion lower end at flat wedge;15min is impregnated into the scion cut into the water, places into the naphthalene of 5mg Acetic acid and 5mg heteroauxin, which are dissolved in the mixed solution that 95% ethanol solution of 1L obtains, impregnates 3min, dries after taking-up;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel is no longer outer It is exposed to female branch section;After scion is plugged, the film for being 2cm with width will bind tight, 40 days tissues after grafting at graft union It dismantles after healing completely;
4. spraying 7 parts of ammonium phosphate, 5 parts of potassium nitrate, 3 parts of ammonium sulfate, 1.5 parts of calcium chloride, 0.8 to stock branch after having grafted Part magnesium sulfate and 0.5 part of boric acid are dissolved in nutrient solution obtained in 800 parts of water, spray every 7 days 1 time, until graft union tissue is cured It closes, realizes normal growth;Simultaneously to whole strain four seasons awns spray 7 parts of carbendazol wettable powders, 3 parts of Te Fuling wettable powders and 1 part of pesticide synergistic agent is dissolved in insect prevention microbial inoculum obtained in 700 parts of water;
(5) management after grafting, comprising:
1. fertilising: apart from mango trunk 60cm at dig width be 30cm, depth for 40cm annular ditch, be put into 15 parts of urea, The mixed fertilizer that 12 parts of potassium chloride, 5 parts of calcium superphosphate, 15 parts of well-rotted farmyard manures form, then the soil of one layer of 8-10cm is covered, depending on fruit tree Growing state is every putting disposable fertilizer the 2-3 month, while the potassium dihydrogen phosphate that spraying concentration is 0.4% continuously sprays 2 to blade face It is secondary, every minor tick 10 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;It is anti-with conventional method Control mango pest and disease damage;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net It pollinates in canopy, after pollination, carries out Routine Management.
It is 5 months from the time for being grafted onto result, and has 91.7% to yield positive results in the branch grafted.By training in 3 years It educates, has filtered out the hybrid new breed of Tai Nongmang and Gui Qimang.
Embodiment 6
A method of shortening mango breeding cycle, comprising the following steps:
(1) acquisition of filial generation seed:
1. choosing 8 years raw four seasons awns as stock tree, pale reddish brown awns and seven awns interval of osmanthus are grafted to the three-level in four seasons awns On major branch, 3 trees are grafted;Brandreth is built in four seasons awns surrounding with steel pipe, takes 40 mesh in heading stage to the small fruiting period of grafting twig Fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is carried out in the flower spike pumping 4-6cm long of grafting twig Pollination after pollination, carries out Routine Management, to obtain the mango hybrid seed of directive breeding;
(2) cultivation of seedling: sterile-processed mango hybrid seed is seeded into July and fills clean river sand In hole tray, seed 1-2cm is crossed with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long to 8- Field planting is transplanted to when 10cm;
(3) stock selects: choosing 8 years raw four seasons awns as parent, selects the diameter of robust growth for 4-6mm branch Female branch as a result, every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 3 trees, each tree Graft 30 branches;Brandreth is built in four seasons awns surrounding with steel pipe, takes 40 mesh insect prevention in heading stage to the small fruiting period of grafting twig Net;
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the November of current year, need to graft from female branch with point of a knife Position is chosen out by bast from xylem;
2. by being cut at the aging healthy and strong terminal bud 3-4cm of 1 tip of mango hybridization seedling extraction, obtained tender tip conduct Scion main bud base portion is whittled into the long bevel that a length is 1-1.5cm by scion on one side, then it is 0.5- that opposite, which is whittled into length, The short bevel of 0.7cm, makes scion lower end at flat wedge;10min is impregnated into the scion cut into the water, places into 2.5mg's Methyl α-naphthyl acetate and 7.5mg heteroauxin, which are dissolved in the mixed solution that 80% ethanol solution of 1L obtains, impregnates 5min, dries in the air after taking-up It is dry;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel is no longer outer It is exposed to female branch section;After scion is plugged, the film for being 1cm with width will bind tight, 30 days tissues after grafting at graft union It dismantles after healing completely;
4. spraying 5 parts of ammonium phosphate, 7 parts of potassium nitrate, 5 parts of ammonium sulfate, 0.8 part of calcium chloride, 0.5 to stock branch after having grafted Part magnesium sulfate and 0.3 part of boric acid are dissolved in nutrient solution obtained in 500 parts of water, spray every 10 days 1 time, until graft union tissue is cured It closes, realizes normal growth;Simultaneously to whole strain four seasons awns spray 5 parts of carbendazol wettable powders, 5 parts of Te Fuling wettable powders and 0.5 part of pesticide synergistic agent is dissolved in insect prevention microbial inoculum obtained in 500 parts of water;
(5) management after grafting, comprising:
1. fertilising: being 20cm digging width at mango trunk 50cm, depth is the annular ditch of 30cm, is put into 10 parts of urea, 7 The mixed fertilizer that part potassium chloride, 7 parts of calcium superphosphate, 10 parts of well-rotted farmyard manures form, then the soil of one layer of 8-10cm is covered, it is raw depending on fruit tree Long situation is every putting disposable fertilizer the 2-3 month, while the potassium dihydrogen phosphate that spraying concentration is 0.2% is to blade face, continuous spray 3 times, Every minor tick 12 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;It is anti-with conventional method Control mango pest and disease damage;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net It pollinates in canopy, after pollination, carries out Routine Management.
It is 5 months from the time for being grafted onto result, and has 90.5% to yield positive results in the branch grafted.By training in 3 years It educates, has filtered out pale reddish brown awns and the hybrid new breed of Gui Qimang.

Claims (8)

1. a kind of method for shortening mango breeding cycle, which comprises the following steps:
(1) acquisition of filial generation seed:
1. choosing the raw four seasons awns of 5-8 as stock tree, two parental breed intervals for being intended to hybridization are grafted in four seasons awns Three-level major branch on, 3-6 tree is grafted in each cross combination;Brandreth is built in four seasons awns surrounding with steel pipe, in grafting twig Heading stage to small fruiting period takes 32-40 mesh fly net;
2. the calliphorid larva near the phase of pupating is put into net canopy and is pollinated in the flower spike pumping 4-6cm long of grafting twig, After pollination, Routine Management is carried out, to obtain the mango hybrid seed of directive breeding;
(2) sterile-processed mango hybrid seed the cultivation of seedling: is seeded into the cave for filling clean river sand in 7-9 month In disk, seed 1-2cm is crossed with fine sand lid after broadcasting, water of drenching, daily water drenching moisturizing later;When mango hybridization seedling is long to 8- Field planting is transplanted to when 10cm;
(3) stock selects: the raw four seasons awns of 5-8 is chosen as parent, selects the branch of robust growth mother's branch as a result, Every is only stayed the 1-2 tip, is trimmed to remaining miscellaneous branch;Each Hybrid grafts 3-6 tree, and each tree grafts 30-50 Branch;Brandreth is built in four seasons awns surrounding with steel pipe, takes 32-40 mesh fly net in heading stage to the small fruiting period of grafting twig;
(4) it grafts:
1. cutting bearing basal shoot from 2-3cm below the tip in the 10-11 month of current year, need to graft from female branch with point of a knife Position is chosen out by bast from xylem;
2. mango hybridization seedling is cut from terminal bud 3-4cm, obtained tender tip is as scion, by scion main bud base portion one side It is whittled into the long bevel that a length is 1-1.5cm, then opposite is whittled into the short bevel that length is 0.5-0.7cm, makes scion lower end At flat wedge;10-15min is impregnated into the scion cut into the water, it is molten to place into the mixing containing methyl α-naphthyl acetate and heteroauxin 3-5min is impregnated in liquid, is dried after taking-up;
3. scion is inserted into the notch of female branch, scion and the cortex of female branch notch is set to match, until long bevel no longer exposes to Female branch section;After scion is plugged, the film for being 1-2cm with width will bind tight at graft union;
4. spraying nutrient solution to stock branch after having grafted, sprayed every 7-10 days 1 time, until graft union organization healing, is realized Normal growth;Insect prevention microbial inoculum is sprayed to whole strain four seasons awns simultaneously;
(5) management after grafting, comprising:
1. fertilising: apart from mango trunk 50-60cm at dig width be 20-30cm, depth for 30-40cm annular ditch, be put into urea, The mixed fertilizer of potassium chloride, calcium superphosphate, well-rotted farmyard manure composition, then the soil of one layer of 8-10cm is covered, it is every depending on fruit tree growth situation Every putting disposable fertilizer the 2-3 month, while the potassium dihydrogen phosphate that spraying concentration is 0.2-0.4% is to blade face, continuous spray 2-3 times, often Minor tick 10-12 days;
2. the prevention and control of plant diseases, pest control: paying attention to trimming clear garden, remove sick branch, the sick leaf on four seasons Mang Shu in time;Awns is prevented and treated with conventional method Fruit disease insect pest;
3. blooming period management: in the flower spike pumping 4-6cm long of grafting twig, the calliphorid larva near the phase of pupating being put into net canopy It pollinates, after pollination, carries out Routine Management.
2. the method according to claim 1 for shortening mango breeding cycle, which is characterized in that the diameter of the bearing basal shoot For 4-6mm.
3. the method according to claim 1 for shortening mango breeding cycle, which is characterized in that the terminal bud is mango hybridization The aging healthy and strong terminal bud of 1 tip of seedling extraction.
4. the method according to claim 1 for shortening mango breeding cycle, which is characterized in that naphthalene second in the mixed solution The mass ratio of acid and heteroauxin is 1-3:1, and the mixed solution is that the methyl α-naphthyl acetate of 10-30mg and heteroauxin are dissolved in 1L Obtained in 80-95% ethanol solution.
5. the method according to claim 1 for shortening mango breeding cycle, which is characterized in that the nutrient solution is 5-7 parts Ammonium phosphate, 5-7 part potassium nitrate, 3-5 parts of ammonium sulfate, 0.8-1.5 parts of calcium chloride, 0.5-0.8 parts of magnesium sulfate and 0.3-0.5 parts of boric acid It is dissolved in obtained in 500-800 parts of water.
6. the method according to claim 1 for shortening mango breeding cycle, which is characterized in that the insect prevention microbial inoculum is 5-7 Part carbendazol wettable powder, 3-5 parts of Te Fuling wettable powders and 0.5-1 parts of pesticide synergistic agents are dissolved in 500-700 parts of water It obtains.
7. the method according to claim 1 for shortening mango breeding cycle, which is characterized in that bound at the graft union Film is dismantled after the 30-40 days tissues healing completely after grafting.
8. the method according to claim 1 for shortening mango breeding cycle, which is characterized in that the mixed fertilizer is by 10-15 Part urea, 7-12 parts of potassium chloride, 5-7 parts of calcium superphosphate, 10-15 parts of well-rotted farmyard manures compositions.
CN201811235376.5A 2018-10-23 2018-10-23 Method for shortening mango breeding cycle Active CN109197206B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811235376.5A CN109197206B (en) 2018-10-23 2018-10-23 Method for shortening mango breeding cycle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811235376.5A CN109197206B (en) 2018-10-23 2018-10-23 Method for shortening mango breeding cycle

Publications (2)

Publication Number Publication Date
CN109197206A true CN109197206A (en) 2019-01-15
CN109197206B CN109197206B (en) 2021-04-20

Family

ID=64981332

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811235376.5A Active CN109197206B (en) 2018-10-23 2018-10-23 Method for shortening mango breeding cycle

Country Status (1)

Country Link
CN (1) CN109197206B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111887092A (en) * 2020-08-14 2020-11-06 河北省农林科学院昌黎果树研究所 Breeding method for two-year-of-one-year-of-breeding of grapes
CN113508747A (en) * 2021-07-19 2021-10-19 云南农业大学 Method for inducing robust growth of mango seedling root system
CN114097445A (en) * 2021-12-28 2022-03-01 中国林业科学研究院经济林研究所 Mutation method for peach-apricot intergeneric grafting hybrid
CN114938752A (en) * 2022-05-26 2022-08-26 中国热带农业科学院南亚热带作物研究所 Mango self-rooting stock cultivation method

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101699970A (en) * 2009-11-17 2010-05-05 广州甘蔗糖业研究所 Method for shortening the production period of mango
CN102599049A (en) * 2012-03-28 2012-07-25 何寒 Pollination method of mango by breeding flies in flowering phase for propagation
CN102742456A (en) * 2012-07-10 2012-10-24 南京农业大学 Method for shortening juvenile span by grafting hybrid seedling rootstocks
CN102860221A (en) * 2012-07-14 2013-01-09 中国热带农业科学院橡胶研究所 Cuttage-grafting seedling raising method for rubber trees
CN102919060A (en) * 2012-11-08 2013-02-13 云南省农业科学院热带亚热带经济作物研究所 Novel method for rainproof grafting of mango saplings or seedlings
CN104855203A (en) * 2015-04-27 2015-08-26 李廷夫 Top grafting canopy replacement method of red mangoes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101699970A (en) * 2009-11-17 2010-05-05 广州甘蔗糖业研究所 Method for shortening the production period of mango
CN102599049A (en) * 2012-03-28 2012-07-25 何寒 Pollination method of mango by breeding flies in flowering phase for propagation
CN102742456A (en) * 2012-07-10 2012-10-24 南京农业大学 Method for shortening juvenile span by grafting hybrid seedling rootstocks
CN102860221A (en) * 2012-07-14 2013-01-09 中国热带农业科学院橡胶研究所 Cuttage-grafting seedling raising method for rubber trees
CN102919060A (en) * 2012-11-08 2013-02-13 云南省农业科学院热带亚热带经济作物研究所 Novel method for rainproof grafting of mango saplings or seedlings
CN104855203A (en) * 2015-04-27 2015-08-26 李廷夫 Top grafting canopy replacement method of red mangoes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李学坚等: "芒果叶药材规范化种植技术", 《中国现代中药》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111887092A (en) * 2020-08-14 2020-11-06 河北省农林科学院昌黎果树研究所 Breeding method for two-year-of-one-year-of-breeding of grapes
CN113508747A (en) * 2021-07-19 2021-10-19 云南农业大学 Method for inducing robust growth of mango seedling root system
CN114097445A (en) * 2021-12-28 2022-03-01 中国林业科学研究院经济林研究所 Mutation method for peach-apricot intergeneric grafting hybrid
CN114097445B (en) * 2021-12-28 2022-10-18 中国林业科学研究院经济林研究所 Mutation method for peach-apricot intergeneric grafting hybrid
CN114938752A (en) * 2022-05-26 2022-08-26 中国热带农业科学院南亚热带作物研究所 Mango self-rooting stock cultivation method
CN114938752B (en) * 2022-05-26 2023-04-28 中国热带农业科学院南亚热带作物研究所 Cultivation method of mango self-root stock

Also Published As

Publication number Publication date
CN109197206B (en) 2021-04-20

Similar Documents

Publication Publication Date Title
CN109197206A (en) A method of shortening mango breeding cycle
CN105766517A (en) Planting method of red juice oranges
CN106804368A (en) A kind of red fuji apple implantation methods
CN105961118A (en) Method for interplanting pasture in camellia oleifera field for raising and stocking poultry
CN108476843A (en) A kind of cultural method that August is fried
CN112205229B (en) Efficient cost-saving citrus cultivation method
CN106034598A (en) Interplanting cultivation method for marigold and konjak
CN108450167A (en) A kind of method of the high-quality stingless Chinese pricklyash of engrafting and cultivating
CN110178625A (en) A kind of management method of fertile mandarin orange bearing-age tree
CN107056354A (en) A kind of high yield dragon fruit implantation methods
CN106386367A (en) Plum tree planting method
CN106171376A (en) A kind of cherry tomato ratooning method
CN104012357A (en) Sand land honey pomelo planting method
CN105660102A (en) Nest sowing seedling reserving and transplanting dual-purpose rape planting method
CN106376322A (en) Saffron crocus seedball propagation method
CN105123179A (en) Oilseed rape ridge culture plantation method
CN105075781A (en) Cultivating method for mulberries
CN113261465B (en) Quick citrus orchard forming method
CN107251804A (en) A kind of implantation methods of apple tree
CN106856965A (en) The method and the Mango Fruit Garden ecosystem of a kind of Mango Fruit Garden planting grass
CN106818388A (en) A kind of lemon orchard zoology breeding method
CN1244338A (en) High-yield litchi cultivation method including grafting one-year old sapling and bearing fruits in two years after grafting
CN112205228B (en) Cultivation method for artificially promoting natural renewal of litsea cubeba
CN109757274A (en) Luohanguo With Plantlets of Tissue Culture high-yield and high-efficiency method for transplanting
CN106665047B (en) Greenhouse melon cultivation method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Su Weiqiang

Inventor after: Liu Yeqiang

Inventor after: Huang Zhangbao

Inventor after: Huang Zhanwei

Inventor after: Zuo Xingjun

Inventor after: Wang Zhitao

Inventor after: Guo Lingfei

Inventor after: Li Hengrui

Inventor after: Lu Meiying

Inventor after: Lu Dimin

Inventor after: Qiu Wenwu

Inventor after: Cai Zhaoyan

Inventor after: Dong Long

Inventor after: Wang Xiaomei

Inventor after: Shi Pingli

Inventor after: Fang Weikuan

Inventor after: Ren Hui

Inventor before: Su Weiqiang

Inventor before: Huang Zhangbao

Inventor before: Guo Lingfei

Inventor before: Lu Meiying

Inventor before: Qiu Wenwu

Inventor before: Liu Yeqiang

Inventor before: Wang Xiaomei

Inventor before: Cai Zhaoyan

Inventor before: Li Hengrui

Inventor before: Dong Long

Inventor before: Fang Weikuan

Inventor before: Ren Hui

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20210316

Address after: 530007, 174 East University Road, the Guangxi Zhuang Autonomous Region, Nanning

Applicant after: HORTICULTURAL Research Institute GUANGXI ACADEMY OF AGRICULTURAL SCIENCES

Applicant after: Baise mango Research Institute

Address before: 530007, 174 East University Road, the Guangxi Zhuang Autonomous Region, Nanning

Applicant before: HORTICULTURAL Research Institute GUANGXI ACADEMY OF AGRICULTURAL SCIENCES

GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20210618

Address after: No.174, daxiangtang University East Road, Nanning, Guangxi 530007

Patentee after: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES

Patentee after: Baise mango Research Institute

Address before: 530007, 174 East University Road, the Guangxi Zhuang Autonomous Region, Nanning

Patentee before: HORTICULTURAL Research Institute GUANGXI ACADEMY OF AGRICULTURAL SCIENCES

Patentee before: Baise mango Research Institute