CN109105307A - A kind of feed and its preparation method and application and the method for breeding of Larimichthys crocea - Google Patents

A kind of feed and its preparation method and application and the method for breeding of Larimichthys crocea Download PDF

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CN109105307A
CN109105307A CN201810984238.0A CN201810984238A CN109105307A CN 109105307 A CN109105307 A CN 109105307A CN 201810984238 A CN201810984238 A CN 201810984238A CN 109105307 A CN109105307 A CN 109105307A
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feed
powder
vitamin
fish
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CN109105307B (en
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叶坤
韩星星
王秋荣
王志勇
陈丰林
柴志强
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Jimei University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
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    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The present invention relates to rheum officinale fish culture field, the method for breeding of a kind of feed and its preparation method and application and Larimichthys crocea is disclosed.The feed contains fish meal, degreasing black soldier flies powder, krill meal, corn protein powder, fermented bean dregs, Gluten, fish oil, wheat flour, alphalise starch, soybean lecithin, vitamin, minerals, calcium dihydrogen phosphate, choline chloride, taurine, cuttlefish cream and preservative, the content of fish meal is 20-40wt%, degreasing black soldier flies powder, fermented bean dregs, the content of Gluten and wheat flour is 5-15wt%, krill meal, corn protein powder, the content of fish oil and alphalise starch is 2-10wt%, the content of soybean lecithin and calcium dihydrogen phosphate is 0.5-3wt%, vitamin, minerals, choline chloride, the content of taurine is 0.5-2wt%, the content of cuttlefish cream is 0.1-1wt%, the content of preservative is 0.01-0.2wt%.After being cultivated using above-mentioned feed to Larimichthys crocea, growth performance, body ingredient and the Biochemical Indices In Serum of Larimichthys crocea, antibacterial, anti-oxidant result are preferable, and feed cost can be saved by reaching, and guarantee the culture efficiency of fish body health.

Description

A kind of feed and its preparation method and application and the method for breeding of Larimichthys crocea
Technical field
The invention belongs to rheum officinale fish culture fields, and in particular to a kind of feed and its preparation method and application and Larimichthys crocea Method for breeding.
Background technique
Fish meal is because containing essential amino acid and fatty acid abundant and more balance, and anti-nutritional factors is few and palatability is good, It is easy the features such as being digested absorption, is always high-quality protein source indispensable in aquatic feeds.The annual ocean fishing in the whole world at present Industry catches about 90,000,000 tons of total amount, wherein being directly used in about 24,000,000 tons of raw material fish of fish meal production, accounts for the 20% of fishing total amount. The processed side product (account for about fishing total amount 6.2%) that global Aquatic Product Process Industry generates is also used for fish meal processing.Therefore, global Fish meal processing industry consumes the 26.2% of global fishery fishing total amount in total.Recently as the continuous hair of World Aquaculture Exhibition, fish meal demand increasingly increases, and due to the decline of fishery resources, supply falls short of demand that its price is caused constantly to rise violently for fish meal.In order to Solve increasingly prominent imbalance between supply and demand, on the one hand can by seek substitute fish meal other protein sources, on the other hand then mention The utilization rate of high forage protein compares the latter, and it is more simple and practical to seek substitution protein sources.Therefore, alternative fish is researched and developed The protein sources of powder are the problems that culture fishery is in the urgent need to address at this stage.
Summary of the invention
The present invention is intended to provide a kind of new feed and its preparation method and application and the method for breeding of Larimichthys crocea.
Feed provided by the invention contains fish meal, degreasing black soldier flies powder, krill meal, corn protein powder, fermented bean dregs, glutelin Powder, fish oil, wheat flour, alphalise starch, soybean lecithin, vitamin, minerals, calcium dihydrogen phosphate, choline chloride, taurine, crow Crafty cream and preservative, on the basis of the total weight of the feed, the content of the fish meal is 20-40wt%, the degreasing Heisui River Horsefly powder, fermented bean dregs, Gluten and wheat flour content be each independently 5-15wt%, the krill meal, corn protein powder, The content of fish oil and alphalise starch is each independently 2-10wt%, and the content of the soybean lecithin and calcium dihydrogen phosphate is respectively only On the spot be 0.5-3wt%, the vitamin, minerals, choline chloride and taurine content be each independently 0.5- 2wt%, the content of the cuttlefish cream are 0.1-1wt%, the content of the preservative is 0.01-0.2wt%, and each component contains The sum of amount is 100wt%.
A preferred embodiment of the invention, on the basis of the total weight of the feed, the content of the fish meal For 25-35wt%, the content of the degreasing black soldier flies powder is 7-14wt%, and the content of the krill meal is 6-8wt%, the jade The content of rice gluten powder is 6-8wt%, and the content of the fermented bean dregs is 8-12wt%, and the content of the Gluten is 8- 14wt%, the content of the fish oil are 4-8wt%, and the content of the wheat flour is 7-12wt%, and the content of the alphalise starch is 4-8wt%, the content of the soybean lecithin are 1-2wt%, and the content of the vitamin is 0.8-1.2wt%, the mineral The content of matter is 0.8-1.2wt%, and the content of the calcium dihydrogen phosphate is 1-2wt%, and the content of the choline chloride is 0.8- 1.5wt%, the content of the taurine are 0.8-1.5wt%, and the content of the cuttlefish cream is 0.2-0.8wt%, the anti-corrosion The content of agent is 0.05-0.15wt%, and the sum of content of each component is 100wt%.
In the present invention, in terms of actual content, remaining ingredient contains the content of the fish oil, soybean lecithin and cuttlefish cream Amount is in terms of dry matter content.
The content of protein is converted into dry weight and is calculated as 58-65wt% in the krill meal.
Term " fermented bean dregs " refers to dregs of beans through the resulting product of microbial fermentation.
Preferably, the weight ratio of the fish meal and degreasing black soldier flies powder is (1.5-5): 1.
Fat content is high in general black soldier flies powder, typically larger than 40wt%, and the present invention is using black after degreasing Stratiomyiid powder, the fish oil for being rich in highly unsaturated fatty acid its object is to supplement addition, meets Larimichthys crocea nutritional need, black soldier flies Since fat content is too high, it is configured to equal rouge feed, fish oil is difficult to add.It is particularly preferred that in the degreasing black soldier flies powder Fat content is 12wt% or less.
Preferably, the vitamin be in multi-vitamins described in multi-vitamins and every kg containing carrotene 0.033g, Vitamin D 0.017g, tocopherol 0.127g, vitamin B10.02g, vitamin B20.063g, vitamin B60.017g, cyanogen cobalt element 0.033mg, biotin 0.003g, inositol 1.283g, niacin 0.257g, pantothenic acid 0.09g, folic acid 0.003g, choline chloride 2.623g and cellulose 0.64g, surplus are the mixture of glycine and glycine betaine according to 1:1.
Preferably, the minerals are to contain sodium fluoride 1mg, potassium iodide in composite mineral matter and the composite mineral matter 0.4mg, CoCL2 6H2O aqueous solution 25mg, cupric sulfate pentahydrate 5mg, the ferrous sulfate 40mg, zinc sulfate that concentration is 1wt% 25mg, manganese sulfate 30mg, epsom salt 600mg, calcium dihydrogen phosphate 1500mg, sodium chloride 50mg and zeolite powder 7.72g.
The preparation method of feed provided by the invention includes being uniformly mixed each component in above-mentioned feed.
Application the present invention also provides the feed as rheum officinale fish meal.
The present invention also provides a kind of method for breeding of Larimichthys crocea, wherein this method includes during the feeding process, in use Feed is stated to be fed.
Preferably, the water temperature of the breeding process is 18.4-23.4 DEG C, and the pH value of water body is 7.8-8.0, and salinity is 27 ‰ -29 ‰, dissolved oxygen > 5mg/L, ammonia nitrogen concentration < 0.05mg/L.
Larimichthys crocea is cultivated using feed provided by the invention, growth performance, body ingredient and the serum of Larimichthys crocea are raw It is preferable to change index, antibacterial and anti-oxidant result, feed cost can be saved in actual production and reach best culture efficiency.
Specific embodiment
The embodiment of the present invention is described below in detail, the examples of the embodiments are intended to be used to explain the present invention, and cannot It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, described according to the literature in the art Technology or conditions or carried out according to product description.Reagents or instruments used without specified manufacturer is that can lead to Cross the conventional products of commercially available acquisition.
In following embodiment and comparative example:
Krill meal is purchased from Xiamen Jia Kang feed corporation,Ltd；
Fermented bean dregs are purchased from Xiamen Jia Kang feed corporation,Ltd；
Vitamin is to contain carrotene 0.033g, vitamin D in multi-vitamins described in multi-vitamins and every kg 0.017g, tocopherol 0.127g, vitamin B10.02g, vitamin B20.063g, vitamin B60.017g, cyanogen cobalt element 0.033mg, Biotin 0.003g, inositol 1.283g, niacin 0.257g, pantothenic acid 0.09g, folic acid 0.003g, choline chloride 2.623g and fibre Element 0.64g is tieed up, surplus is the mixture of glycine and glycine betaine according to 1:1；
Minerals are in composite mineral matter and the composite mineral matter The CoCL2 6H2O aqueous solution 25mg of 1wt%, cupric sulfate pentahydrate 5mg, ferrous sulfate 40mg, zinc sulfate 25mg, manganese sulfate 30mg, Epsom salt 600mg, calcium dihydrogen phosphate 1500mg, sodium chloride 50mg and zeolite powder 7.72g；
Preservative is sodium Diacetate；
Fat content in degreasing black soldier flies powder is 11.25wt%.
Preparation example
Preparation example with fish meal, degreasing Heisui River gadfly powder be main protein sources, using fish oil as fat source, with degreasing Heisui River gadfly Powder substitute respectively 0% (control group), 20%, 40%, 60%, 80%, 100% fish meal be configured to the nitrogen (CP:45%) such as 6 kinds and The test feed of equal rouge (CL:10%), feed formula composition see the table below 1:
1 test feed of table composition and nutritional ingredient (wt%)
Feed G0 G20 G40 G60 G80 G100
Fish meal 42 33.6 25.2 16.8 8.4 0
Degreasing black soldier flies powder 0 7 14 21 28 35
Krill meal 5 5 5 5 5 5
Corn protein powder 5 5 5 5 5 5
Fermented bean dregs 10 10 10 10 10 10
Gluten 8 9.6 13.5 17.6 22 25.5
Fish oil 5.5 5.6 5.5 5.5 5.5 5.5
Wheat flour 11.4 11.1 8.7 6 3 0.9
Alphalise starch 5 5 5 5 5 5
Soybean lecithin 1.5 1.5 1.5 1.5 1.5 1.5
Multidimensional 1 1 1 1 1 1
More mines 1 1 1 1 1 1
Calcium dihydrogen phosphate 1.5 1.5 1.5 1.5 1.5 1.5
Choline chloride 1 1 1 1 1 1
Taurine 1 1 1 1 1 1
Cuttlefish cream 0.5 0.5 0.5 0.5 0.5 0.5
Preservative 0.1 0.1 0.1 0.1 0.1 0.1
Chrome green 0.5 0.5 0.5 0.5 0.5 0.5
It is total 100 100 100 100 100 100
Crude protein 45.27±0.35 45.05±0.52 45.00±0.26 45.06±0.53 45.30±0.63 44.98±0.75
Crude fat 10.26±0.96 10.46±0.86 10.45±0.99 10.53±0.73 10.52±0.89 10.7±0.6
Coarse ash 9..54±0.04 10.22±0.02 10.52±0.43 11.21±0.08 11.25±0.05 11.33±0.06
Note: in table 1, chrome green acts as the effect of indicator, the purpose of addition be in order to which follow-up test is prepared, G0, G20, G40, G60, G80, G100 are respectively indicated: control group substitutes 20% fish meal group, substitution using degreasing black soldier flies powder 40% fish meal group, 60% fish meal group of substitution, 80% fish meal group of substitution, 100% fish meal group of substitution, similarly hereinafter.
Embodiment
Larimichthys crocea is carried out feeding cultivation respectively using above six kinds of feeds (G0, G20, G40, G60, G80 and G100), often In triplicate, by 120 tail Larimichthys croceas (50.08 ± 3.31g), cement pit (2m × 1m × 1m) carries out 7 weeks feeding to group indoors every time Grow test.Feed is fed during test daily 2 times (morning 6:00 and afternoon 18:00) until Larimichthys crocea does not rob food, every time Feed soil pick-up after 1h, every morning 7:30 changes that water is primary, and water temperature is 18.4-23.4 DEG C, pH value 7.8-8.0 during test, salt Degree is 27 ‰ -29 ‰, dissolved oxygen > 5mg/L, ammonia nitrogen concentration < 0.05mg/L.Wherein, each group feed is to Larimichthys crocea growth performance Influence be shown in Table 2, the influence to rheum officinale nutrition components is shown in Table 3, and the influence to Larimichthys crocea Biochemical Indices In Serum is shown in Table 4, to rheum officinale Fish is anti-oxidant and the influence of antibacterial ability is shown in Table 5.
Influence of the 2 each group feed of table to Larimichthys crocea growth performance
Fish meal is substituted to Larimichthys crocea survival rate without significant shadow using degreasing Heisui River gadfly powder it can be seen from the result of table 2 It rings, in addition to 100% substitution (G100), other each group test group (G0, G20, G40, G60 and G80) fish survival rates are 82% More than, with the raising of substitution ratio, survival rate, rate of body weight gain, specific growth rate, protein efficiency ratio, PER all present first to increase to be declined afterwards Trend, while G20 and G40 rate of body weight gain, protein efficiency ratio, PER are significantly higher than control group (P < 0.05), and feed coefficient is substantially less than pair According to group (P < 0.05).
Influence (dry matter basis) of the 3 each group feed of table to muscles of Pseudosciaena crocea trophic component
Project 0 20 40 60 80 100
Crude protein 62.64±2.11a 63.42±2.17a 60.28±2.5ab 55.38±2.22b 49.91±3.54bc 46.14±1.92c
Crude fat 27.37±0.45b 29.46±0.8ab 31.51±0.66a 27.16±0.88b 25.57±0.5c 30.37±0.49a
Coarse ash 6.60±0.65b 6.57±0.50b 6.24±0.52b 7.34±0.39ab 7.78±0.72ab 8.61±0.09a
Using degreasing Heisui River gadfly powder substitution fish meal to crude protein in muscles of Pseudosciaena crocea it can be seen from the result of table 3 It influences, G0, G20, G40 difference is not significant (P > 0.05), and G60, G80, G100 content are on a declining curve, and G100 group test fish flesh Meat crude protein content is minimum, substantially less than other test groups (P < 0.05).Crude fat content in addition to G80 group significantly reduce (P < 0.05), G20 and G40 group is all remarkably higher than (P < 0.05) control group.With the rising of degreasing black soldier flies powder ratio, content of ashes The trend risen after falling before is presented, and G100 group content of ashes is significantly higher than control group (P < 0.05).
Influence of the 4 each group feed of table to Larimichthys crocea Biochemical Indices In Serum
Project G0 G20 G40 G60 G80 G100
TP (g/L) total protein 20.65±0.35b 21.10±0.14a 21.00±0.14ab 17.45±0.07d 17.5±0d 19.2±0c
ALB (g/L) albumin 2.20±0.14b 2.50±0a 2.35±0.07ab 1.90±0c 1.95±0.07c 2.30±0b
GLOB (g/L) globulin 18.45±0.49a 18.60±0.14a 18.65±0.21a 15.55±0.07c 15.55±0.07c 16.90±0b
A/G Archon ratio 0.12±0.01 0.13±0 0.13±0 0.12±0 0.13±0 0.14±0
ALT (u/L) glutamic-pyruvic transaminase 6.50±0.71d 13.50±0.71b 16.50±2.12a 9.50±0.71c 9.00±0c 14.00±0b
A/G is between each group without significant difference in serum it can be seen from the result of table 4.TP, ALB, GLOB, ALT are in each group Between significant difference (P < 0.05).With the increase of substitution ratio, TP, ALB, GLOB content are first increased in each group test fish serum After reduce and increase again, and reach maximum value in G20 or G40 group.GLOB difference is not significant in G0, G20, G40 group, ALT in each group All it is significantly higher than control group (P < 0.05) and maximum value appears in G40 group.
5 each group feed of table is anti-oxidant to Larimichthys crocea and the influence of antibacterial ability
As shown in table 5, T-AOC, SOD are presented first to rise and are declined afterwards as substitution ratio rises in Antioxidant Indexes Trend, and reach maximum value in G20 or G40 group, wherein G20 group T-AOC is significantly higher than control group (P < 0.05), G20 group CAT with Control group is compared, and difference is not significant, and MDA, LZM presentation are fallen before, rear ascendant trend, and difference is not significant (P > 0.05).
As can be seen from the above results, Larimichthys crocea is cultivated using G20-G40, the growth performance of Larimichthys crocea, body at Divide and Biochemical Indices In Serum, antibacterial, anti-oxidant result are preferable, shadow will not be caused to Larimichthys crocea juvenile growth performance in a short time It rings, feed cost can be saved in actual production and reach best culture efficiency.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance In the case where shield, it can be combined in any appropriate way.In order to avoid unnecessary repetition, the present invention to it is various can No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally The thought of invention, it should also be regarded as the disclosure of the present invention.

Claims (10)

1. a kind of feed, which is characterized in that the feed contains fish meal, degreasing black soldier flies powder, krill meal, corn protein powder, hair Ferment dregs of beans, Gluten, fish oil, wheat flour, alphalise starch, soybean lecithin, vitamin, minerals, calcium dihydrogen phosphate, chlorination gallbladder Alkali, taurine, cuttlefish cream and preservative, on the basis of the total weight of the feed, the content of the fish meal is 20-40wt%, The degreasing black soldier flies powder, fermented bean dregs, Gluten and wheat flour content be each independently 5-15wt%, the krill Powder, corn protein powder, fish oil and alphalise starch content be each independently 2-10%, the soybean lecithin and calcium dihydrogen phosphate Content be each independently 0.5-3wt%, the vitamin, minerals, the content of choline chloride and taurine are respectively independent Ground is 0.5-2wt%, and the content of the cuttlefish cream is 0.1-1wt%, and the content of the preservative is 0.01-0.2wt%, and each The sum of content of component is 100wt%, and the content of the fish oil, soybean lecithin and cuttlefish cream is in terms of actual content, remaining set The content divided is in terms of dry matter content.
2. feed according to claim 1, which is characterized in that on the basis of the total weight of the feed, the fish meal Content is 25-35wt%, and the content of the degreasing black soldier flies powder is 7-14wt%, and the content of the krill meal is 6-8wt%, institute The content for stating corn protein powder is 6-8wt%, and the content of the fermented bean dregs is 8-12wt%, and the content of the Gluten is 8- 14wt%, the content of the fish oil are 4-8wt%, and the content of the wheat flour is 7-12wt%, and the content of the alphalise starch is 4-8wt%, the content of the soybean lecithin are 1-2wt%, and the content of the vitamin is 0.8-1.2wt%, the mineral The content of matter is 0.8-1.2wt%, and the content of the calcium dihydrogen phosphate is 1-2wt%, and the content of the choline chloride is 0.8- 1.5wt%, the content of the taurine are 0.8-1.5wt%, and the content of the cuttlefish cream is 0.2-0.8wt%, the anti-corrosion The content of agent is 0.05-0.15wt%, and the sum of content of each component is 100wt%, the fish oil, soybean lecithin and cuttlefish The content of cream is in terms of actual content, and the content of remaining ingredient is in terms of dry matter content.
3. feed according to claim 1, which is characterized in that the weight ratio of the fish meal and degreasing black soldier flies powder is (1.5-5): 1.
4. feed described in any one of -3 according to claim 1, which is characterized in that the fat in the degreasing black soldier flies powder Content is 12wt% or less.
5. feed described in any one of -3 according to claim 1, which is characterized in that the vitamin be multi-vitamins and Contain carrotene 0.033g, vitamin D 0.017g, tocopherol 0.127g, vitamin B in multi-vitamins described in every kg1 0.02g, vitamin B20.063g, vitamin B60.017g, cyanogen cobalt element 0.033mg, biotin 0.003g, inositol 1.283g, Buddhist nun Gram acid 0.257g, pantothenic acid 0.09g, folic acid 0.003g, choline chloride 2.623g and cellulose 0.64g, surplus are glycine and sweet tea Dish alkali according to 1:1 mixture.
6. feed described in any one of -3 according to claim 1, which is characterized in that the minerals be composite mineral matter and In the composite mineral matter containing sodium fluoride 1mg, potassium iodide 0.4mg, concentration be 1wt% CoCL2 6H2O aqueous solution 25mg, five Brochanite 5mg, ferrous sulfate 40mg, zinc sulfate 25mg, manganese sulfate 30mg, epsom salt 600mg, calcium dihydrogen phosphate 1500mg, sodium chloride 50mg and zeolite powder 7.72g.
7. the preparation method of feed described in any one of claim 1-6, which is characterized in that this method includes wanting right The each component in feed described in any one of 1-6 is asked to be uniformly mixed.
8. application of the feed as rheum officinale fish meal described in any one of claim 1-6.
9. a kind of method for breeding of Larimichthys crocea, which is characterized in that this method includes during the feeding process, using claim 1-6 Any one of described in feed fed.
10. method for breeding according to claim 9, wherein the water temperature of the breeding process is 18.4-23.4 DEG C, water body PH value be 7.8-8.0, salinity be 27 ‰ -29 ‰, dissolved oxygen > 5mg/L, ammonia nitrogen concentration < 0.05mg/L.
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