CN107114616A - A kind of selenium-rich rainbow trout feed and its preparation method and application - Google Patents

A kind of selenium-rich rainbow trout feed and its preparation method and application Download PDF

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CN107114616A
CN107114616A CN201710479076.0A CN201710479076A CN107114616A CN 107114616 A CN107114616 A CN 107114616A CN 201710479076 A CN201710479076 A CN 201710479076A CN 107114616 A CN107114616 A CN 107114616A
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feed
selenium
vitamin
rainbow trout
raw material
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张学振
王力
吴雷
田大才
刘琪
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Huazhong Agricultural University
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
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    • A23K20/22Compounds of alkali metals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
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    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract

The present invention relates to aquatic feeds field, and in particular to a kind of selenium-rich rainbow trout feed and its preparation method and application.The feed of the present invention includes peru fish meal, Swine blood meal, krill meal, chicken meal, corn protein powder, dregs of beans, soybean protein isolate, Gluten, pre-gelatinized starch, Squid Oil, soybean lecithin, glycine betaine, Choline Chloride, mineral substance premix, vitamin premix and yeast selenium.Using the forage feed rainbow trout of the present invention, raised through 70 days, the total Se content of rainbow trout muscle(Weight in wet base)It can reach 0.57 1.12 mg/kg, the complete total Se content of fish(Weight in wet base)It can reach 0.58 1.06 mg/kg.Rainbow trout using the feed cultivation of the present invention has preferable growth performance, efficiency of feed utilization and meat quality;In addition, the selenium-rich rainbow trout feed of the present invention also has effect of liver and gallbladder protection, enhancing rainbow trout oxidation resistance and nonspecific mental retardation.

Description

A kind of selenium-rich rainbow trout feed and its preparation method and application
Technical field
The present invention relates to aquatic products field, and in particular to a kind of selenium-rich rainbow trout feed and its preparation method and application.
Background technology
Selenium, since being found from 1817 by Sweden chemist Berzelius, has been considered as one kind in a very long time Toxic element.Until nineteen fifty-seven, Schwarz and Foltz have found to suppress the master for the hepatonecrosis factor III that rat is triggered by feed Want just to contain selenium in composition, selenium is just gradually recognized as the identity of required trace element in animal body by people.Then, greatly Quantifier elimination shows, selenium plays key player in various normal body functions, and selenium deficiency will cause Keshan disease, white muscle disease with And a variety of diseases such as angiocardiopathy.
Selenium is widely distributed in soil, can be converted by plant absorption, and animal and the selenium of the mankind are controlled by food chain Nutrition condition, therefore the selemium nutrition situation of the mankind and the distribution of selenium be closely related.Although China there are the title of " world's selenium is all " Bestow favour, but the Selenium in Soil resource distribution of China is seriously uneven, and China, which has one, includes black, the lucky, province of LIAODONG PENISULA ON NORTH three area, The North Chinas such as Beijing, Hebei, Shandong, the Inner Mongol, Gansu etc. is northern and northwestern, the southwest ground such as cloud, expensive, river and Tibet Including area by the southwest low selenium area in northeast.How to solve the problems, such as that the selenium-supply of the low selenium area people increasingly attracts attention.
The existence form of selenium is divided into inorganic selenium and the major class of Organic Selenium two, and inorganic selenium includes selenate, selenite etc., inorganic Selenium is for animal and people, and bioavailability is relatively low, and risk of toxicity is big, and improper use easily causes selenosis.And Organic Selenium The selenium with biomolecule combination of shape and state is then referred mainly to, can be by inorganic selenium be through animals and plants, microorganism sorption enhanced, including seleno Methionine, selenocysteine etc., the bioavailability of Organic Selenium in animal body are high, small toxicity, are more suitable for selenium-supply requirement.
With the discovery successively of selenium various beneficial functions in vivo, the desire of people's selenium-supply is also increasingly stronger, with And come the research and development on selenium-enriched food it is also more and more.Fish are originated as a kind of food proteins of high-quality, rich on it The research of selenium product is also relatively more, the selenium-rich organic that such as Application No. CN201010579798.1 Chinese patent is announced Fish and its breeding method, the selenium-rich for being used to cultivate selenium-rich fish that the Chinese patent of Application No. 201110392618.3 is announced are raised Material and its preparation method and application, the Se-enriched feedstuff that these patents are announced can cultivate selenium-rich fish, but various different fishes Class has different nutritional needs, therefore these patents are not necessarily applied to present invention rainbow trout of interest.In addition, application number A kind of selenium-rich cold water fish forage is disclosed for 201410532760.7 Chinese patent, although the Feed Energy that the invention is provided exists Rainbow trout muscle is set to reach Se accumulation in a short time, but its feed Se content is of a relatively high (5-20mg/kg), and numerous studies show, to the greatest extent Pipe exists in Organic Selenium form, and higher feed Se content can still cause toxic action to rainbow trout, reduce rainbow trout growth performance, All many-sides such as feed conversion and immunity function, reduce yield, and this invention is not made to the health status of rainbow trout in itself and commented Valency.Therefore it is simple in order to reach selenium-rich purpose, and the underproduction that too high feed Se content is brought is have ignored, it will lose more than gain.Cause This, obtains a kind of safety, and the Se-enriched feedstuff being efficiently adapted to again with rainbow trout is extremely urgent.
The content of the invention
In view of the above-mentioned problems, it is an object of the invention to provide a kind of selenium-rich rainbow trout feed, the feed is ensuring cultivating Growth performance, feed conversion and meat quality of rainbow trout etc. will not be adversely affected while going out selenium-rich rainbow trout, it is ensured that close Suitable selenium addition, it is to avoid be excessively added, so as to bring the underproduction and the decline of fish meat.
It is also an object of the present invention to provide a kind of preparation method of selenium-rich rainbow trout feed, method is simple, easy.
Final object of the present invention is a kind of application of selenium-rich rainbow trout feed in selenium-rich rainbow trout feed is prepared.
To achieve the above object, technical scheme is as follows:
A kind of selenium-rich rainbow trout feed, including:
Peru fish meal 300-350g
Swine blood meal 40-70g
Krill meal 30-50g
Chicken meal 120-180g
Corn protein powder 40-60g
Dregs of beans 50-80g
Soybean protein isolate 40-60g
Gluten 30-50g
Pre-gelatinized starch 30-50g
Squid Oil 100-140g
Soybean lecithin 3-7g
Glycine betaine 3-7g
Choline Chloride 0.8-1.2g
Mineral substance premix 35-50g
Vitamin premix 2-3g
Selenium 1.65-7.00mg
Include per 1000g mineral substance premixes:Five water lactic acid calcium 350g, dipotassium hydrogen phosphate 250g, a water calcium dihydrogen phosphate 175g, sodium chloride 75g, KI 0.125g, cupric sulfate pentahydrate 37.5g, manganese sulfate monohydrate 2.5g, monohydrate zinc sulphate 14.05g and Potassium carbonate 35g, remaining is auxiliary material.
Include per 1000g vitamin premixs:Vitamin A 60g, vitamin B1 8g, vitamin B2 12g, vitamin B3 8g, vitamin B5 24g, vitamin B6 12g, vitamin B7 0.4g, vitamin B12 0.02g, vitamin C 600g, dimension life Plain D3 0.04g, vitamin E 96g, Vitamin K3 8g and folic acid 4g, remaining is auxiliary material.
The selenium is Organic Selenium, the addition in the form of yeast selenium preferably;
Described auxiliary material is the customary adjuvant of this area, preferably alpha-cellulose.
In schemes described above, it is preferred that selenium-rich rainbow trout feed includes:
Peru fish meal 334g
Swine blood meal 50g
Krill meal 40g
Chicken meal 150g
Corn protein powder 50g
Dregs of beans 60g
Soybean protein isolate 50g
Gluten 40g
Pre-gelatinized starch 40g
Squid Oil 130g
Soybean lecithin 5g
Glycine betaine 5g
Choline Chloride 1g
Mineral substance premix 40g
Vitamin premix 2.5g
Selenium 4mg.
A kind of preparation method of selenium-rich rainbow trout feed, including:
1) all of above component was crushed into 60 mesh sieves;
2) preparation of vitamin premix:The height of each group vitamin content in being matched respectively by vitamin premix, from It is low to high, raw material is added into auxiliary material one by one, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of adding for raw material Plus, until the addition of all raw materials is finished, fully mix, both obtain vitamin premix;
3) preparation of mineral substance premix:Respectively press mineral substance premix in each group vitamin content height, from it is low to Height, is added raw material into auxiliary material one by one, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of addition of raw material, Until the addition of all raw materials is finished, fully mix, both obtain mineral substance premix;
4) according to raw material proportion in feed formula, in addition to Squid Oil, remaining raw material is contained by each component in feed formula The height of amount, is added from low to high, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of addition of raw material, directly Finished to the addition of all raw materials, fully mix, obtain mixture 1;
5) Squid Oil is added into mixture 1, and is well mixed, mixture 2 is obtained;
6) 0.25kg clear water is added into every kg mixtures 2, is well mixed, obtains mixture 3;
7) mixture 3 is expanded through 75 DEG C, granulation both obtains feed granules;
8) obtained feed granules are placed in into ventilation to spread out, it is to avoid sunlight irradiates, natural air drying, it is ensured that moisture < 10%, both selenium-rich rainbow trout feed of the invention.
A kind of application of selenium-rich rainbow trout feed in selenium-rich rainbow trout feed is prepared, the feed provided using the present invention is fed Rainbow trout is supported, or rainbow trout is fed with other feed formulations.
Compared to existing technical scheme, it is an advantage of the invention that:
The selenium-rich rainbow trout feed of the present invention is designed in strict accordance with the nutritional need of rainbow trout, and nutrition is balanced, and palatability is good, energy The nutritional need of rainbow trout normal growth is met, and adds krill meal and glycine betaine as phagostimulant, promotes fish.Also, The effect of selenium-rich rainbow trout feed of the present invention not only has liver and gallbladder protection, and the oxidation resistance of rainbow trout and non-spy can also be strengthened Specific immunological function.Feed base nutritional ingredient is as shown in table 1:
A kind of selenium-rich rainbow trout feed nutrient table of table 1
The present invention adds selenium source for emerging organic selenium source yeast selenium, and small toxicity, bioavailability is high;
The addition of strict control yeast selenium of the invention, on the premise of selenium-rich rainbow trout is cultivated, it is ensured that the health of rainbow trout, most Limits ensure growth performance, feed conversion and meat.
The selenium-rich rainbow trout feed of the present invention has liver and gallbladder protection effect, and can lift the anti-oxidant of rainbow trout to a certain extent Ability and nonspecific mental retardation.
Rainbow trout is cultivated using feed provided by the present invention 70 days, and the total Se content of rainbow trout muscle (in terms of weight in wet base) can reach 0.57-1.12mg/kg, full fish Se content can reach 0.58-1.06mg/kg.
Embodiment
The claim of the present invention is described in further detail with reference to embodiment, but not constituted to this Any limitation of invention, the still modification of anyone limited number of time made within the scope of the invention as claimed, the power in the present invention Within the scope of profit is claimed.Technical scheme of the present invention, is the ordinary skill in the art if not otherwise specified;It is used Reagent or material, if not otherwise specified, derive from commercial channel.
Embodiment 1:
A kind of selenium-rich rainbow trout feed (feed 1), including:
Include per 1000g mineral substance premixes:Five water lactic acid calcium 350g, dipotassium hydrogen phosphate 250g, a water calcium dihydrogen phosphate 175g, sodium chloride 75g, KI 0.125g, cupric sulfate pentahydrate 37.5g, manganese sulfate monohydrate 2.5g, monohydrate zinc sulphate 14.05g and Potassium carbonate 35g, remaining is auxiliary material.
Include per 1000g vitamin premixs:Vitamin A 60g, vitamin B1 8g, vitamin B2 12g, vitamin B3 8g, vitamin B5 24g, vitamin B6 12g, vitamin B7 0.4g, vitamin B12 0.02g, vitamin C 600g, dimension life Plain D3 0.04g, vitamin E 96g, Vitamin K3 8g and folic acid 4g, remaining is auxiliary material.
The selenium is added in the form of yeast selenium, the yeast selenium that the present invention is used, and is determined through inductive coupling plasma mass spectrometry, Its Se content is 4000mg/kg;
Described auxiliary material is alpha-cellulose.
A kind of selenium-rich rainbow trout feed (feed 2), including:
Remaining is identical with feed 1.
A kind of selenium-rich rainbow trout feed (feed 3), including:
Remaining is identical with feed 1.
A kind of preparation method of selenium-rich rainbow trout feed, including:
1) component of feed 1, feed 2 or feed 3 was crushed into 60 mesh sieves;
2) preparation of vitamin premix:The height of each group vitamin content in being matched respectively by vitamin premix, from It is low to high, raw material is added into alpha-cellulose one by one, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of raw material Addition, until all raw materials addition finish, fully mix, both vitamin premix;
3) preparation of mineral substance premix:Respectively press mineral substance premix in each group vitamin content height, from it is low to Height, is added raw material into alpha-cellulose one by one, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of adding for raw material Plus, until the addition of all raw materials is finished, fully mix, both obtain mineral substance premix;
4) according to raw material proportion in feed formula, in addition to Squid Oil, remaining raw material is contained by each component in feed formula The height of amount, is added from low to high, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of addition of raw material, directly Finished to the addition of all raw materials, fully mix, obtain mixture 1;
5) Squid Oil is added into mixture 1, and is well mixed, mixture 2 is obtained;
6) 0.25kg clear water is added into every kg mixtures 2, is well mixed, obtains mixture 3;
7) mixture 3 is expanded through 75 DEG C, granulation both obtains feed granules;
Obtained feed granules are placed in into ventilation to spread out, it is to avoid sunlight irradiates, natural air drying, it is ensured that moisture < 10%, Both selenium-rich rainbow trout feed is obtained.
According to above-mentioned formula and method, three kinds of different Se-enriched feedstuffs of Se content, respectively feed 1, the and of feed 2 have been made Feed 3.For following examples.
Embodiment 2:
A kind of application of selenium-rich rainbow trout feed in selenium-rich rainbow trout is cultivated:
The present embodiment carries out culture experiment using 3 kinds of feeds obtained in embodiment 1 as experiment feed.Experiment starts Before, the dry of feed 1~3, crude protein, crude fat, ash content and total Se content are determined, measurement result such as table 2:
Table 2 tests feed proximate component and total Se content
Before experiment starts, 180 tail average weights are put into 9 mouthfuls of annular plastics cylinders at random for 144.87 ± 26.45g rainbow trout In (1000L), 9 mouthfuls of cylinders are randomly divided into 3 groups, and hungry 24 hours of all fishes are simultaneously weighed one by one.After experiment starts, 4 groups of fishes are per natural gift It is not satiated with food and feeds four kinds of experiment feeds twice (9:00 and 16:00) food ration, is recorded in detail, and water temperature is controlled in 9-11 DEG C, dissolved oxygen 6.5±0.5mg/L.After 70 days cultivate, stop eating 24 hours, and weigh one by one, amount body is long.And 6 tail fishes are taken at random per cylinder, its In 3 tails take in muscle samples collection, and weigh liver and visceral mass weight, remaining 3 tail is used for full fish index determining.All knots Fruit carries out one-way analysis of variance by SPSS 19.0 (SPSS Inc., Chicago, IL, USA), with significant difference In the case of carry out Duncan ' s Multiple range tests, significance is set as 0.05.
Rainbow trout growth performance and feed conversion are as shown in table 3:
The growth performance of table 3 and feed conversion
Experimental result is shown, in three treatment groups, and that there are no significant is poor for the growth of rainbow trout items and feed conversion index of correlation It is different, illustrate the safe model that the yeast selenium pitch-based sphere in a kind of selenium-rich rainbow trout feed provided by the present invention can adapt in rainbow trout In enclosing, toxic action is not caused to rainbow trout, so as to reduce rainbow trout growth performance and feed conversion.In this experiment, each group rainbow trout Survival rate is 100%, illustrates that the basal feed and Se-enriched feedstuff of the present invention disclosure satisfy that the primary demand of rainbow trout, can guarantee that rainbow The survival of trout;In addition, the feed coefficient of feed 1,2,3 has reached that less than 1.4, particularly feed 2 have reached 1.27, illustrate this The selenium-rich rainbow trout feed that invention is provided has higher feed conversion rate, can be digested and assimilated well by rainbow trout, and this experiment Cultivation temperature used is 9-11 DEG C, relatively low, so the speed of growth of fish is slower, if used in more preferable water temperature The selenium-rich rainbow trout feed of the present invention, feed coefficient can also be reduced.These results illustrate, the equal energy of selenium-rich rainbow trout feed of the invention The growth demand of rainbow trout is met well, and efficiency of feed utilization is higher, and added yeast Se content can bear in rainbow trout Safe range in, it is non-toxic to rainbow trout, feed the life that a kind of selenium-rich rainbow trout feed provided by the present invention does not interfere with rainbow trout Long performance, feed conversion and meat quality, and bring the underproduction and the decline of meat quality.
Rainbow trout muscle and the total Se content of full fish are as shown in table 4:
The muscle of table 4 and the total Se content of full fish (mg/kg weight in wet bases)
In per a line, different Superscript letters are represented with significant difference (P < 0.05).
By the cultivation of 70 days, feed 1 provided by the present invention, feed 2, feed 3 significantly improves rainbow trout muscle and complete The total Se content of fish, and the increase of muscle and total Se content of full fish has dosage effect, illustrates selenium-rich provided by the present invention Rainbow trout feed can have preferable selenium-rich effect.
Rainbow trout meat quality is as shown in table 5
The meat quality of table 5
In per a line, different letters represent significant difference (P < 0.05).
As a result show, in this experiment, in addition to drip loss, remaining meat quality index of correlation there are no significant difference is said The selenium addition of bright selenium-rich rainbow trout feed provided by the present invention does not reduce the meat quality of rainbow trout, the selenium that the present invention is recommended Addition is acceptable.Although each index (in addition to drip loss) there are no significant difference, from the numerical result of indices From the point of view of, in terms of meat quality, the pitch-based sphere using the every kg feeds of 4mg selenium is optimal.
To sum up, this example demonstrates that a kind of selenium-rich rainbow trout feed provided by the present invention disclosure satisfy that the normal need of rainbow trout Ask, it is ensured that survival rate, and with higher feed conversion rate, can well be absorbed by rainbow trout;The selenium-rich rainbow of the present invention The selenium addition of trout feed is not made in the safe range of rainbow trout to the growth performance of rainbow trout, feed conversion and meat quality Into adverse effect, and when adding 4mg selenium levels with every kg feeds, the meat quality of rainbow trout is up to optimal.
Embodiment 3:
In order to further illustrate a kind of feeding effect of selenium-rich rainbow trout feed provided by the present invention, the present embodiment is by supporting Grow experiment and the contrast of culture efficiency has been carried out with a kind of commercial feed to a kind of selenium-rich rainbow trout feed of the present invention.
Before experiment starts, 480 tail average weights are put into 6 mouthfuls of annular plastics cylinders at random for 12.37 ± 0.03g rainbow trout In (1000L), 6 mouthfuls of cylinders are randomly divided into 2 groups, and hungry 24 hours of all fishes are simultaneously weighed one by one.After experiment starts, 2 groups of fishes are per natural gift It is not satiated with food and feeds a kind of commercially available commercial feed and the feed 2 obtained by embodiment 1, feeds daily 3 times (7:00,12:30 and 18: 00) food ration, is recorded in detail, and water temperature is controlled in 16-17 DEG C, 8.0 ± 0.5mg/L of dissolved oxygen.After 30 days cultivate, stop eating 24 small When, and weigh one by one, amount body is long.Take 5 tail fishes at random per cylinder in addition, gather serum, measurement liver and visceral mass weight.All knots Fruit carries out independent samples t test by SPSS 19.0 (SPSS Inc., Chicago, IL, USA), and significance is set as 0.05。
Growth and feed conversion result are as shown in table 6:
Table 6 grows and feed conversion
* represent that same data line has significant difference (P < 0.05).
Growth and feed conversion result show that the survival rate of rainbow trout is 100% in 2 groups of feed, higher than commercial feed group;Raise Material 2 significantly increases the rate of body weight gain and specific growth rate of rainbow trout, illustrates that the rainbow trout that feed provided by the present invention is raised has More preferable growth performance;Also, the feed coefficient of feed 2 is lower than commercial feed, and numerically illustrate institute of the present invention less than 1 The efficiency of feed utilization of the selenium-rich rainbow trout feed of offer is high, can be utilized well by rainbow trout.
Serum related enzyme activity index result is as shown in table 7:
The serum related enzyme activity index of table 7
* represent that same data line has significant difference (P < 0.05).
In serum, the change of liver function of the reacting condition of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease activity, when liver is impaired, Gpt activity in serum can be lifted with glutamic-oxalacetic transaminease activity, in this experiment, compared to commercial feed, feed feed 2 significantly reduce the glutamic-oxalacetic transaminease activity of rainbow trout serum, illustrate the selenium-rich rainbow trout feed of the present invention compared to commercially available business rainbow Trout Feed Energy preferably safeguards rainbow trout liver function, the effect of with liver and gallbladder protection;In the anti-oxidant relevant enzyme of serum, feed 2 is notable The total superoxides enzymatic activity of rainbow trout is improved, illustrates feed of the invention relative to commercially available business rainbow trout feed for the anti-of rainbow trout Oxidability has certain lifting, and mda content reflects level of lipid, is significantly dropped in the rainbow trout serum for feeding feed 2 Low mda content also demonstrate that lifting of the selenium-rich rainbow trout feed for rainbow trout oxidation resistance of the present invention;Lysozyme, alkali The acid phosphatase enzyme related to acid phosphatase right and wrong specific immunity, feed 2 significantly enhances rainbow trout Serum Basic phosphoric acid Enzyme and activity of acid phosphatase, illustrate that the selenium-rich rainbow trout feed for feeding the present invention is same for the nonspecific mental retardation of rainbow trout In the presence of certain humidification.
To sum up, the present embodiment further illustrates a kind of selenium-rich rainbow trout feed provided by the present invention compared to commercially available business Feed has more excellent performance.

Claims (6)

1. a kind of selenium-rich rainbow trout feed, including:
Peru fish meal 300-350g
Swine blood meal 40-70g
Krill meal 30-50g
Chicken meal 120-180g
Corn protein powder 40-60g
Dregs of beans 50-80g
Soybean protein isolate 40-60g
Gluten 30-50g
Pre-gelatinized starch 30-50g
Squid Oil 100-140g
Soybean lecithin 3-7g
Glycine betaine 3-7g
Choline Chloride 0.8-1.2g
Mineral substance premix 35-50g
Vitamin premix 2-3g
Selenium 1.65-7.00mg;
Include per 1000g mineral substance premixes:Five water lactic acid calcium 350g, dipotassium hydrogen phosphate 250g, a water calcium dihydrogen phosphate 175g, Sodium chloride 75g, KI 0.125g, cupric sulfate pentahydrate 37.5g, manganese sulfate monohydrate 2.5g, monohydrate zinc sulphate 14.05g and carbonic acid Potassium 35g, remaining is auxiliary material;
Include per 1000g vitamin premixs:Vitamin A 60g, vitamin B1 8g, vitamin B2 12g, vitamin B3 8g, Vitamin B5 24g, vitamin B6 12g, vitamin B7 0.4g, vitamin B12 0.02g, vitamin C 600g, vitamine D3 0.04g, vitamin E 96g, Vitamin K3 8g and folic acid 4g, remaining is auxiliary material;
The selenium is Organic Selenium.
2. feed according to claim 1, described selenium is added in the form of yeast selenium.
3. feed according to claim 1, described auxiliary material is alpha-cellulose.
4. feed according to claim 1, it is characterised in that:
Peru fish meal 334g
Swine blood meal 50g
Krill meal 40g
Chicken meal 150g
Corn protein powder 50g
Dregs of beans 60g
Soybean protein isolate 50g
Gluten 40g
Pre-gelatinized starch 40g
Squid Oil 130g
Soybean lecithin 5g
Glycine betaine 5g
Choline Chloride 1g
Mineral substance premix 40g
Vitamin premix 2.5g
Selenium 4mg.
5. a kind of preparation method of selenium-rich rainbow trout feed, including:
1)The all components of feed described in claim 1 were crushed into 60 mesh sieves;
2)The preparation of vitamin premix:Respectively by vitamin premix match in each group vitamin content height, from it is low to Height, is added raw material into auxiliary material one by one, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of addition of raw material, Until the addition of all raw materials is finished, fully mix, both obtain vitamin premix;
3)The preparation of mineral substance premix:The height of each group vitamin content in mineral substance premix is pressed respectively, from low to high, by One is added raw material into auxiliary material, is mixed rapidly after often adding a kind of raw material, then carries out down a kind of addition of raw material, until institute There is raw material addition to finish, fully mix, both obtain mineral substance premix;
4)According to raw material proportion in feed formula, in addition to Squid Oil, remaining raw material presses each component content in feed formula Just, it is added, is mixed rapidly after often adding a kind of raw material from low to high, then carry out down a kind of addition of raw material, until institute There is raw material addition to finish, fully mix, obtain mixture 1;
5)Squid Oil is added into mixture 1, and is well mixed, mixture 2 is obtained;
6)0.25kg clear water is added into every kg mixtures 2, is well mixed, obtains mixture 3;
7)Mixture 3 is expanded through 75 DEG C, and granulation both obtains feed granules.
6. application of the feed in selenium-rich rainbow trout feed is prepared described in claim 1.
CN201710479076.0A 2017-06-22 2017-06-22 A kind of selenium-rich rainbow trout feed and its preparation method and application Pending CN107114616A (en)

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