CN108925990A - 一种富含多种矿物质的益生菌铁锌钙粉及其制备方法 - Google Patents
一种富含多种矿物质的益生菌铁锌钙粉及其制备方法 Download PDFInfo
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- CN108925990A CN108925990A CN201810880812.8A CN201810880812A CN108925990A CN 108925990 A CN108925990 A CN 108925990A CN 201810880812 A CN201810880812 A CN 201810880812A CN 108925990 A CN108925990 A CN 108925990A
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- probiotics
- vitamin
- iron zinc
- calcium powder
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
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- A—HUMAN NECESSITIES
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明公开了一种富含多种矿物质的益生菌铁锌钙粉,其原料包括:食用葡萄糖、乳粉、益生菌、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3。本发明还公开了上述一种富含多种矿物质的益生菌铁锌钙粉的制备方法。本发明的益生菌铁锌钙粉含有丰富的铁锌钙元素,还含有丰富的氨基酸、葡萄糖、维生素、膳食纤维,能够满足人体的各种营养物质的吸收,营养价值全面,且营养物质释放效率高,易吸收。
Description
技术领域
本发明涉及保健食品的技术领域,尤其涉及一种富含多种矿物质的益生菌铁锌钙粉及其制备方法。
背景技术
铁、锌、钙均是人体所必需的微量元素,尤其对特殊人群如婴幼儿、儿童、青少年的生长发育,对孕妇和乳母的健康都具有重要意义。虽然上述微量元素在体内的含量很低,但是它们在酶活性的降低或丧失,激素、蛋白质和维生素的合成和代谢中起着关键的作用,缺失其中的一种都会对人体健康造成极大的危害。例如:缺钙容易发生软骨病、出牙迟、牙齿稀松、鸡胸、身体矮小、“X”形腿和畸形等佝偻病症状;缺铁会使细胞色素和含铁酶的活性减弱,导致供氧不足、能量代谢紊乱和免疫功能下降,甚至会出现贫血症状;缺锌会出现生长发育迟缓、智力下降、精神萎靡和厌食等症状,严重时还会造成缺锌性侏儒。
益生菌是一类对宿主有益的活性微生物,其定植于人体肠道、生殖***内,可改善宿主微生态平衡、发挥有益作用,随着人们生活水平的提高,益生菌的保健功效也被普遍接受并广泛应用。
目前,市面上的铁、锌、钙复合产品以及益生菌的产品越来越多样化。市面上的铁、锌、钙复合产品一般只注重营养成分的搭配,对钙、铁、锌复合产品的口感和稳定性研究的较少,导致目前铁、锌、钙复合产品的口感和稳定性较差,不利于铁、锌、钙复合产品的推广和应用,而益生菌产品的原料也比较单一,营养物质无法满足实际食用的需求,故此亟需开发一种富含多种矿物质的益生菌铁锌钙粉来解决现有技术中的问题。
发明内容
为解决背景技术中存在的技术问题,本发明提出一种富含多种矿物质的益生菌铁锌钙粉及其制备方法,含有丰富的铁锌钙元素,还含有丰富的氨基酸、葡萄糖、维生素、膳食纤维,能够满足人体的各种营养物质的吸收,营养价值全面,且营养物质释放效率高,易吸收。
本发明提出的一种富含多种矿物质的益生菌铁锌钙粉,其原料按包括:食用葡萄糖、乳粉、益生菌、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3。
进一步的,其原料按重量份包括:食用葡萄糖15-35份、乳粉30-34份、益生菌26-30份、水苏糖2-5份、低聚异麦芽糖1-5份、DHA藻油3-6份、膳食纤维4-8份、乳酸亚铁3-8份、葡萄糖酸锌2-4份、乳酸钙1-3份、牛磺酸3-5份、维生素A 2-5份、维生素B2 0.1-0.4份、维生素C 0.05-0.15份、维生素D3 0.02-0.06份。
进一步的,所述益生菌为乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌按重量比1-3:2-5:1-4:3-6:4-8混合而成。
进一步的,其原料按重量份包括缓释助剂10-20份。
进一步的,所述缓释助剂的原料按重量份包括:壳聚糖4-8份、壳寡糖3-9份、纤维蛋白胶5-15份、透明质酸4-9份。
进一步的,所述缓释助剂按如下工艺进行制备:将壳聚糖于1100-1130℃,熔炼0.5-1.5h,然后降温至600-700℃,保温45-55h,接着于70-80℃,水浴搅拌65-75h,接着于750-850℃煅炼4-6h,研磨至50-150μm颗粒,冷却至室温得到物料a;将壳寡糖、纤维蛋白胶和透明质酸混合均匀,于350-650r/min转速搅拌20-30min,接着于-30~-50℃冷冻干燥机中干燥20-40min得到缓释助剂。
本发明提出的一种富含多种矿物质的益生菌铁锌钙粉的制备方法,包括如下步骤:
S1:益生菌的活化:将乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌五种菌种分别接种到四个MRS斜面培养基上,于30-37℃培养10-16h,得到活化的斜面菌种;
S2、扩大培养:将所得斜面菌种分别接种于MRS液体培养基中,30-37℃培养10-16h,得到液体菌种,然后将五中液体菌种分别按0.2-0.5%(v/v)的接种量接入灭菌的培养液中,30-37℃培养1-2d,得菌种扩大液;
S3、发酵:以食用葡萄糖、乳粉、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3、缓释助剂为原料制备发酵培养基,将其灭菌后按3-5%(v/v)的接种量接种菌种扩大液,30-37℃密封培养1-2d,至pH降至3.5-4.0时终止发酵;
S4、冷冻干燥:将发酵产物过滤,所得发酵液经冷冻干燥制得所述益生菌铁锌钙粉。
本发明以乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌五种菌作为益生菌接种培养后进行扩大培养得到菌种扩大液,接着以食用葡萄糖、乳粉、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3、缓释助剂为原料制备发酵培养基,接着将菌种扩大液于发酵培养基上进行发酵处理,接着冷冻干燥得到,不仅含有丰富的益生菌,还含有丰富的营养物质,营养物质释放效率高,营养物质吸收效果好,能够有效满足人体对各种营养物质的需求。
具体实施方式
下面结合具体实施例对本发明做出详细说明,应当了解,实施例只用于说明本发明,而不是用于对本发明进行限定,任何在本发明基础上所做的修改、等同替换等均在本发明的保护范围内。
在实施例中,所述益生菌铁锌钙粉,其原料按重量份包括:食用葡萄糖15-35份、乳粉30-34份、益生菌26-30份、水苏糖2-5份、低聚异麦芽糖1-5份、DHA藻油3-6份、膳食纤维4-8份、乳酸亚铁3-8份、葡萄糖酸锌2-4份、乳酸钙1-3份、牛磺酸3-5份、维生素A 2-5份、维生素B2 0.1-0.4份、维生素C 0.05-0.15份、维生素D3 0.02-0.06份、缓释助剂10-20份。
所述益生菌为乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌按重量比1-3:2-5:1-4:3-6:4-8混合而成。
所述缓释助剂按如下工艺进行制备:按重量份将4-8份壳聚糖于1100-1130℃,熔炼0.5-1.5h,然后降温至600-700℃,保温45-55h,接着于70-80℃,水浴搅拌65-75h,接着于750-850℃煅炼4-6h,研磨至50-150μm颗粒,冷却至室温得到物料a;将3-9份壳寡糖、5-15份纤维蛋白胶和4-9份透明质酸混合均匀,于350-650r/min转速搅拌20-30min,接着于-30~-50℃冷冻干燥机中干燥20-40min得到缓释助剂。
本发明还提供一种富含多种矿物质的益生菌铁锌钙粉的制备方法,包括如下步骤:
S1:益生菌的活化:将乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌五种菌种分别接种到四个MRS斜面培养基上,于30-37℃培养10-16h,得到活化的斜面菌种;
S2、扩大培养:将所得斜面菌种分别接种于MRS液体培养基中,30-37℃培养10-16h,得到液体菌种,然后将五中液体菌种分别按0.2-0.5%(v/v)的接种量接入灭菌的培养液中,30-37℃培养1-2d,得菌种扩大液;
S3、发酵:以食用葡萄糖、乳粉、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3、缓释助剂为原料制备发酵培养基,将其灭菌后按3-5%(v/v)的接种量接种菌种扩大液,30-37℃密封培养1-2d,至pH降至3.5-4.0时终止发酵;
S4、冷冻干燥:将发酵产物过滤,所得发酵液经冷冻干燥制得所述益生菌铁锌钙粉。
实施例1
一种富含多种矿物质的益生菌铁锌钙粉,其原料按重量份包括:食用葡萄糖25份、乳粉32份、益生菌28份、水苏糖3.5份、低聚异麦芽糖3份、DHA藻油4.5份、膳食纤维6份、乳酸亚铁5.5份、葡萄糖酸锌3份、乳酸钙2份、牛磺酸4份、维生素A3.5份、维生素B2 0.25份、维生素C 0.1份、维生素D3 0.04份、缓释助剂15份。
所述益生菌为乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌按重量比2:3.5:2.5:4.5:6混合而成。
所述缓释助剂按如下工艺进行制备:按重量份将6份壳聚糖于1120℃,熔炼1h,然后降温至650℃,保温50h,接着于75℃,水浴搅拌70h,接着于800℃煅炼5h,研磨至100μm颗粒,冷却至室温得到物料a;将6份壳寡糖、10份纤维蛋白胶和6.5份透明质酸混合均匀,于550r/min转速搅拌25min,接着于-40℃冷冻干燥机中干燥30min得到缓释助剂。
本发明还提供一种富含多种矿物质的益生菌铁锌钙粉的制备方法,包括如下步骤:
S1:益生菌的活化:将乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌五种菌种分别接种到四个MRS斜面培养基上,于33℃培养13h,得到活化的斜面菌种;
S2、扩大培养:将所得斜面菌种分别接种于MRS液体培养基中,34℃培养13h,得到液体菌种,然后将五中液体菌种分别按0.35%(v/v)的接种量接入灭菌的培养液中,33℃培养1d,得菌种扩大液;
S3、发酵:以食用葡萄糖、乳粉、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3、缓释助剂为原料制备发酵培养基,将其灭菌后按4%(v/v)的接种量接种菌种扩大液,33℃密封培养2d,至pH降至3.6时终止发酵;
S4、冷冻干燥:将发酵产物过滤,所得发酵液经冷冻干燥制得所述益生菌铁锌钙粉。
实施例2
一种富含多种矿物质的益生菌铁锌钙粉,其原料按重量份包括:食用葡萄糖15份、乳粉34份、益生菌26份、水苏糖5份、低聚异麦芽糖1份、DHA藻油6份、膳食纤维4份、乳酸亚铁8份、葡萄糖酸锌2份、乳酸钙3份、牛磺酸3份、维生素A5份、维生素B2 0.1份、维生素C 0.15份、维生素D3 0.02份、缓释助剂20份。
所述益生菌为乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌按重量比1:5:1:6:4混合而成。
所述缓释助剂按如下工艺进行制备:按重量份将4份壳聚糖于1130℃,熔炼0.5h,然后降温至700℃,保温45h,接着于80℃,水浴搅拌65h,接着于850℃煅炼4h,研磨至150μm颗粒,冷却至室温得到物料a;将3份壳寡糖、15份纤维蛋白胶和4份透明质酸混合均匀,于650r/min转速搅拌20min,接着于-50℃冷冻干燥机中干燥20min得到缓释助剂。
本发明还提供一种富含多种矿物质的益生菌铁锌钙粉的制备方法,包括如下步骤:
S1:益生菌的活化:将乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌五种菌种分别接种到四个MRS斜面培养基上,于30℃培养16h,得到活化的斜面菌种;
S2、扩大培养:将所得斜面菌种分别接种于MRS液体培养基中,30℃培养16h,得到液体菌种,然后将五中液体菌种分别按0.2%(v/v)的接种量接入灭菌的培养液中,37℃培养1d,得菌种扩大液;
S3、发酵:以食用葡萄糖、乳粉、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3、缓释助剂为原料制备发酵培养基,将其灭菌后按3%(v/v)的接种量接种菌种扩大液,37℃密封培养1d,至pH降至4.0时终止发酵;
S4、冷冻干燥:将发酵产物过滤,所得发酵液经冷冻干燥制得所述益生菌铁锌钙粉。
实施例3
一种富含多种矿物质的益生菌铁锌钙粉,其原料按重量份包括:食用葡萄糖35份、乳粉30份、益生菌30份、水苏糖2份、低聚异麦芽糖5份、DHA藻油3份、膳食纤维8份、乳酸亚铁3份、葡萄糖酸锌4份、乳酸钙1份、牛磺酸5份、维生素A2份、维生素B2 0.4份、维生素C 0.05份、维生素D3 0.06份、缓释助剂10份。
所述益生菌为乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌按重量比3:2:4:3:8混合而成。
所述缓释助剂按如下工艺进行制备:按重量份将8份壳聚糖于1100℃,熔炼1.5h,然后降温至600℃,保温55h,接着于70℃,水浴搅拌75h,接着于750℃煅炼6h,研磨至50μm颗粒,冷却至室温得到物料a;将9份壳寡糖、5份纤维蛋白胶和9份透明质酸混合均匀,于350r/min转速搅拌30min,接着于-30℃冷冻干燥机中干燥40min得到缓释助剂。
本发明还提供一种富含多种矿物质的益生菌铁锌钙粉的制备方法,包括如下步骤:
S1:益生菌的活化:将乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌五种菌种分别接种到四个MRS斜面培养基上,于30℃培养16h,得到活化的斜面菌种;
S2、扩大培养:将所得斜面菌种分别接种于MRS液体培养基中,30℃培养16h,得到液体菌种,然后将五中液体菌种分别按0.2%(v/v)的接种量接入灭菌的培养液中,37℃培养1d,得菌种扩大液;
S3、发酵:以食用葡萄糖、乳粉、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3、缓释助剂为原料制备发酵培养基,将其灭菌后按5%(v/v)的接种量接种菌种扩大液,30℃密封培养2d,至pH降至3.5时终止发酵;
S4、冷冻干燥:将发酵产物过滤,所得发酵液经冷冻干燥制得所述益生菌铁锌钙粉。
以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,根据本发明的技术方案及其发明构思加以等同替换或改变,都应涵盖在本发明的保护范围之内。
Claims (7)
1.一种富含多种矿物质的益生菌铁锌钙粉,其特征在于,其原料按包括:食用葡萄糖、乳粉、益生菌、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3。
2.根据权利要求1所述的益生菌铁锌钙粉,其特征在于,其原料按重量份包括:食用葡萄糖15-35份、乳粉30-34份、益生菌26-30份、水苏糖2-5份、低聚异麦芽糖1-5份、DHA藻油3-6份、膳食纤维4-8份、乳酸亚铁3-8份、葡萄糖酸锌2-4份、乳酸钙1-3份、牛磺酸3-5份、维生素A 2-5份、维生素B2 0.1-0.4份、维生素C 0.05-0.15份、维生素D3 0.02-0.06份。
3.根据权利要求1或2所述的益生菌铁锌钙粉,其特征在于,所述益生菌为乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌按重量比1-3:2-5:1-4:3-6:4-8混合而成。
4.根据权利要求1-3任一项所述的益生菌铁锌钙粉,其特征在于,其原料按重量份包括缓释助剂10-20份。
5.根据权利要求1-4任一项所述的益生菌铁锌钙粉,其特征在于,所述缓释助剂的原料按重量份包括:壳聚糖4-8份、壳寡糖3-9份、纤维蛋白胶5-15份、透明质酸4-9份。
6.根据权利要求1-5任一项所述的益生菌铁锌钙粉,其特征在于,所述缓释助剂按如下工艺进行制备:将壳聚糖于1100-1130℃,熔炼0.5-1.5h,然后降温至600-700℃,保温45-55h,接着于70-80℃,水浴搅拌65-75h,接着于750-850℃煅炼4-6h,研磨至50-150μm颗粒,冷却至室温得到物料a;将壳寡糖、纤维蛋白胶和透明质酸混合均匀,于350-650r/min转速搅拌20-30min,接着于-30~-50℃冷冻干燥机中干燥20-40min得到缓释助剂。
7.一种根据权利要求1-6任一项所述的益生菌铁锌钙粉的制备方法,其特征在于,包括如下步骤:
S1:益生菌的活化:将乳双歧杆菌、嗜酸乳杆菌、干酪乳杆菌、长双歧杆菌、植物乳杆菌五种菌种分别接种到四个MRS斜面培养基上,于30-37℃培养10-16h,得到活化的斜面菌种;
S2、扩大培养:将所得斜面菌种分别接种于MRS液体培养基中,30-37℃培养10-16h,得到液体菌种,然后将五中液体菌种分别按0.2-0.5%(v/v)的接种量接入灭菌的培养液中,30-37℃培养1-2d,得菌种扩大液;
S3、发酵:以食用葡萄糖、乳粉、水苏糖、低聚异麦芽糖、DHA藻油、膳食纤维、乳酸亚铁、葡萄糖酸锌、乳酸钙、牛磺酸、维生素A、维生素B2、维生素C、维生素D3、缓释助剂为原料制备发酵培养基,将其灭菌后按3-5%(v/v)的接种量接种菌种扩大液,30-37℃密封培养1-2d,至pH降至3.5-4.0时终止发酵;
S4、冷冻干燥:将发酵产物过滤,所得发酵液经冷冻干燥制得所述益生菌铁锌钙粉。
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