CN108774086A - A kind of peach foliage bacterial fertilizer and the preparation method and application thereof - Google Patents
A kind of peach foliage bacterial fertilizer and the preparation method and application thereof Download PDFInfo
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- CN108774086A CN108774086A CN201810704685.6A CN201810704685A CN108774086A CN 108774086 A CN108774086 A CN 108774086A CN 201810704685 A CN201810704685 A CN 201810704685A CN 108774086 A CN108774086 A CN 108774086A
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- peach
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of peach foliage bacterial fertilizers and the preparation method and application thereof, with 20-40 parts of Bacillus cercus microbial inoculum, 4-8 parts of chitosan, 4-8 parts of amino acid, micro- 10-20 parts of fertilizer, 20-40 parts of water.Applied to the growth etc. for increasing the yield of peach, improving peach quality, improving peach storage performance, induce enhancing peach disease resistance, promote peach plant.Have many advantages, such as significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease, improves yield and quality, is of low cost and easy to spread.
Description
Technical field
The present invention relates to peach cultivation techniques and fertilising field, and in particular to a kind of peach foliage bacterial fertilizer and preparation method thereof
With application.
Background technology
Peach fruit delicious flavour, it is full of nutrition, it is one of the fresh fruit that people like the most.In addition to fresh food, it can also be processed into peach
Dried meat, peach butter, peach juice, dried peach and peach can.Also there is many parts of peach medical value, root, leaf, flower, benevolence can be used as medicine, and have
Have a functions such as cough-relieving, promoting blood circulation, defaecation, peach kernel oil content 45% can squeeze iundustrial oil, peach-pit hard shell can preparing active carbon, be more
The raw material of industry of purposes.Peach is early with result, high yield stability is good, and less strict, cultivation management is required to edaphic condition
The features such as being easy and cultivation apple, other deciduous fruit trees such as pears compare, and can faster be more easy to acquisition economic benefit, thus especially by
To the favor of grower.But with the development of industry, administrative skill level is backward, serious, yield and quality is low etc. occurs for pest and disease damage
Outstanding problem is presented, and then it is extremely urgent to establish high-quality, efficient, ecological, safe peach corresponding cultivation techniques.Foliage bacterial fertilizer is logical
It crosses after being sprayed onto plant leaf surface and can be absorbed by plant rapidly by plant leaf blade, avoided from nutrient when soil application
Easily by soil consolidation, root absorption is slow the problems such as, have many advantages, such as to absorb that fast, nutrient loss is few.Currently, existing blade face bacterium
There are many kinds of fertilizer, also has certain effect in the yield for improving crop, but all different degrees of presence improves crop yield
The defects of effect is not notable enough and there are no the ideal foliage bacterial fertilizers designed exclusively for peach in the prior art.
Invention content
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, meets peach at significant effect
The peach foliage bacterial fertilizer of growing nutrient demand.
Technical scheme is as follows：
A kind of peach foliage bacterial fertilizer, it is made from the following raw materials in parts by weight：It is prepared from the following ingredients in percentage by group
At：20-40 parts of Bacillus cercus microbial inoculum, 4-8 parts of chitosan, 4-8 parts of amino acid, micro- 10-20 parts of fertilizer, 20-40 parts of water.
Preferably, it is made from the following raw materials in parts by weight：30 parts of Bacillus cercus microbial inoculum, 6 parts of chitosan, amino acid
6 parts, fertile 15 parts micro-, 43 parts of water.
Preferably, the strain contained in Bacillus cercus microbial inoculum is Bacillus cercus LY05 (CCTCC NO:M
2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more).
Wherein, Bacillus cercus microbial inoculum preparation includes the following steps：(1) by bacillus cereus strain LY05, LGY06
Or the inclined-planes WL08 preserve test tube seed, are seeded in minimal medium respectively, shaken cultivation to exponential phase, obtain bacterium
Liquid；(2) bacterium solution of acquisition is seeded to the seed fermentation containing minimal medium with the inoculum concentration of 10% percent by volume
In tank, culture to exponential phase obtains seed liquor；(3) seed liquor of acquisition is connect according to the inoculum concentration of 10% volume ratio
Kind enter fermentation medium in, fermented and cultured 2-3 days at 30-35 DEG C, 150-200rpm, acquisition zymotic fluid, zymotic fluid it is effective
Viable count >=5,000,000,000/mL, zymotic fluid directly dilute 5-20 times as single bacterial strain liquid bacterial agent；(4) by wax-like gemma bar
The single bacterial strain liquid bacterial agent of bacteria strain LY05, LGY06 or WL08 are mixed final by a combination of one or more
Bacillus cercus mix bacterium agent.
Further, the chitosan is water solubility, and deacetylation is more than 95%；The amino acid is proline, sweet ammonia
Acid, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine,
Arbitrary five kinds or more of combination in valine, alanine, serine, arginine, asparatate, cysteine；It is described
Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium of 0.5-1
The mixed fertilizer of+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer；The water is sterile water.
The preparation method of the peach foliage bacterial fertilizer of the present invention, comprises the technical steps that：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportioning, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportioning by regulation proportioning；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH6.0-6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to peach bacterial fertilizer products.
The bio-bacterial manure of the present invention can be applied in following (1)-(4) are at least one：
(1) increase the yield of peach；(2) improve peach quality, improve peach storage performance；(3) induction enhancing peach is anti-
Characteristic of disease, the diseases such as effective prevention and control peach shothole disease, rot disease, brown spot；(4) promote the growth of peach plant.
Compared with prior art, the present invention foliage bacterial fertilizer of the present invention using Bacillus cercus microbial inoculum, plant growth-promoting substance,
The compositions such as a great number of elements and trace element can effectively improve peach economic benefit, nutrient and flavour quality and storage performance, promote to plant
The good growth of strain, and peach plant, the enhancing of fruit disease resistance can be effectively induced, there is significant effect, green non-pollution, fertilizer profit
With rate height, prevention and control disease pest, improve yield and quality, it is of low cost and easy to spread the advantages that.
Specific implementation mode
With reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1：
1. bio-bacterial manure is formed and is prepared：
Bio-bacterial manure A formula compositions and preparation, (1) composition：30 parts of Bacillus cercus microbial inoculum, 6 parts of chitosan, amino acid
6 parts, fertile 15 parts micro-, 43 parts of water.(2) it prepares：1. Bacillus cercus microbial inoculum is taken by regulation proportioning, it is spare；2. being matched by regulation
It takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred to be dissolved in the required sterile water matched；3. 1. microbial inoculum that step is obtained is added
In 2. solution that step obtains, under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, it is sufficiently mixed in sterile blender
It is even, it is packed up to peach foliage bacterial fertilizer A.
Foliar fertilizer B formula compositions and preparation, (1) composition：6 parts of chitosan, 6 parts of amino acid, fertile 15 parts micro-, 73 parts of water.(2)
It prepares：1. taking chitosan, amino acid and micro- fertilizer to be sufficiently stirred by regulation proportioning to be dissolved in the required sterile water matched, peach is made
Foliar fertilizer B.
2. field trial：
Experimental field overview：Experimental field it is located at Pingba County, Guizhou peach garden, kind is " bright red peach ".It is for examination orchard soil type
Yellow earth, as soil background, pH value is random in orchard before experiment, multiple spot mixing acquisition depth 0-60cm soil sample
5.77, total nitrogen content 1.45gkg-1, content of organic matter 29.43gkg-1, alkaline hydrolysis nitrogen content is 98.54mgkg-1,
Available phosphorus content is 7.43mgkg-1。
Field experimental design：RANDOMIZED BLOCK DESIGN is taken in experiment, using complete stool (including blade face positive and negative, limb, fruit
Deng) spraying, if 100-200 times of liquid of foliage bacterial fertilizer, 100 times of liquid of foliar fertilizer, spray clear water are control (CK), totally 3 processing are often located
5 trees of reason, are repeated 4 times, 12 cells totally, around set protection row.Administration time is peach leaf stretching period, setting phase, nutriment
The accumulation phase applies respectively, amount of application is respectively 30,45,60kg/ mus.Weather is based on fine during experiment, or it is cloudy, spray
Afterwards without rainfall in 3-4d.
3. analysis project and method：
After fruit maturation, is harvested by cell, measure each cell single plant yield, 200 fruits of each cell random acquisition,
Take back experimental determination fruit quality, single fruit weight.Nutrient quality assay method：Dry matter is measured with National Standard Method method；Vitamin C contains
Amount is measured with 2,6 dichlorophenol indophenol method；Titratable acid (TA) content determination of acid-basetitration；Soluble solid (SSC) content
It is measured with PAL-1 type refractometers.Storage time is all rotted the time using stored peach.
Incidence and maturation history：It is every 2 days investigation fruit incidences to start softening in fruit, counts accumulative morbidity
Rate；And investigate peach plant diseases incidence.Incidence (%)=100 × (hair the number of sick fruits/investigation the total number of fruits) or incidence
(%)=100 × (morbidity strain number/investigation strain number).The growth indexes such as peach plant leaf SPAD values are observed 7-8 months in fertilising.
4. embodiment effect：
From table 1 to table 4 as can be seen that can effectively increase single plant yield, mean fruit weight using peach foliage bacterial fertilizer,
Fruit dry matter, vitamin C and soluble solid (SSC) content are significantly improved, titratable acid (TA) content is reduced, promotes peach
Set the improvement of the formation and quality of yield；And the hardness of fruit can be effectively improved and maintain, fruit weight-loss ratio is reduced, peach is improved
Fruit storage property；The generation of peach disease can also effectively be reduced；The growth of peach plant can be effectively facilitated.
Influence of the application foliage bacterial fertilizer of table 1 to expensive long peach yield and quality
Note：With marking different lowercase letters after column data in P<0.05 time difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 time difference is same up to pole level of signifiance following tables
2 peach foliage bacterial fertilizer storage performance of table is investigated
A situation arises to expensive long peach disease for the application of table 3 foliage bacterial fertilizer
4 peach garden of table uses foliage bacterial fertilizer plant growth condition
The specific application example of the above only present invention, the present invention also has other embodiments, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection domain of the presently claimed invention.
Claims (7)
1. a kind of peach foliage bacterial fertilizer, it is characterised in that：It is comprised the following components in percentage by weight：Bacillus cercus bacterium
20-40 parts of agent, 4-8 parts of chitosan, 4-8 parts of amino acid, micro- 10-20 parts of fertilizer, 20-40 parts of water.
2. peach foliage bacterial fertilizer according to claim 1, it is characterised in that：It is made from the following raw materials in parts by weight：Wax
30 parts of shape gemma bacillus agent, 6 parts of chitosan, 6 parts of amino acid, fertile 15 parts micro-, 43 parts of water.
3. peach foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：Contain in said waxy gemma bacillus agent
Some strains are Bacillus cercus LY05 (CCTCC NO:M 2015397), Bacillus cercus LGY06 (CCTCC NO:M
And Bacillus cercus WL08 (CCTCCNO 2015398):M2015399 a combination of one or more).
4. peach foliage bacterial fertilizer according to claim 3, it is characterised in that：It is prepared by said waxy gemma bacillus agent
Following steps：(1) inclined-planes bacillus cereus strain LY05, LGY06 or WL08 are preserved into test tube seed, be seeded to respectively inorganic
In salt culture medium, shaken cultivation to exponential phase obtains bacterium solution；(2) by the bacterium solution of acquisition with 10% percent by volume
Inoculum concentration is seeded in the seed fermentation tank containing minimal medium, and culture to exponential phase obtains seed liquor；(3) will
The seed liquor of acquisition is inoculated with according to the inoculum concentration of 10% volume ratio in fermentation medium, at 30-35 DEG C, 150-200rpm
Fermented and cultured 2-3 days, obtains zymotic fluid, the living bacteria count of zymotic fluid >=5,000,000,000/mL, zymotic fluid is directly diluted 5-20 times
As single bacterial strain liquid bacterial agent；(4) by the single bacterial strain liquid bacterial agent of bacillus cereus strain LY05, LGY06 or WL08
Final Bacillus cercus mix bacterium agent is mixed and made by a combination of one or more.
5. peach foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：The chitosan is water solubility, deacetylated
Degree is more than 95%；The amino acid be proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, lysine,
Threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate, half
Arbitrary five kinds or more of combination in cystine；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:
0.5-1:0.5-1:The mixed fertilizer of potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1；It is described
Water is sterile water.
6. a kind of preparation method of peach foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that：Including following technique
Step：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportioning, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportioning by regulation proportioning；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, the item of pH6.0-6.8
Under part, it is sufficiently mixed in sterile blender uniformly, it is packed up to peach bacterial fertilizer products.
7. a kind of application of peach foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that including the following aspects
Using：(1) increase the yield of peach；(2) improve peach quality, improve peach storage performance；(3) induction enhancing peach is disease-resistant
Property, disease of effective prevention and control including peach shothole disease, rot disease, brown spot；(4) promote the growth of peach plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810704685.6A CN108774086A (en) | 2018-06-30 | 2018-06-30 | A kind of peach foliage bacterial fertilizer and the preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810704685.6A CN108774086A (en) | 2018-06-30 | 2018-06-30 | A kind of peach foliage bacterial fertilizer and the preparation method and application thereof |
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| Publication Number | Publication Date |
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| CN108774086A true CN108774086A (en) | 2018-11-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810704685.6A Withdrawn CN108774086A (en) | 2018-06-30 | 2018-06-30 | A kind of peach foliage bacterial fertilizer and the preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114402984A (en) * | 2022-02-07 | 2022-04-29 | 临沧市林业科学院 | Seedling raising method for macadamia nut fruit trees |
-
2018
- 2018-06-30 CN CN201810704685.6A patent/CN108774086A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114402984A (en) * | 2022-02-07 | 2022-04-29 | 临沧市林业科学院 | Seedling raising method for macadamia nut fruit trees |
| CN114402984B (en) * | 2022-02-07 | 2023-12-05 | 临沧市林业科学院 | Seedling raising method for macadimia nut fruit trees |
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Application publication date: 20181109 |