CN114402984A - Seedling raising method for macadamia nut fruit trees - Google Patents
Seedling raising method for macadamia nut fruit trees Download PDFInfo
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- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 7
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- 239000000758 substrate Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 2
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- 235000014571 nuts Nutrition 0.000 description 28
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
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- 239000004475 Arginine Substances 0.000 description 3
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 3
- 235000003704 aspartic acid Nutrition 0.000 description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 3
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- 238000003786 synthesis reaction Methods 0.000 description 3
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- 238000001914 filtration Methods 0.000 description 1
- XEMZLVDIUVCKGL-UHFFFAOYSA-N hydrogen peroxide;sulfuric acid Chemical compound OO.OS(O)(=O)=O XEMZLVDIUVCKGL-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
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- 239000012286 potassium permanganate Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Soil Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of macadimia nut planting, in particular to a macadimia nut fruit tree seedling raising method which comprises the following steps: placing a microbial agent in the planting matrix, and then fixing the macadimia nut seedlings in the planting matrix; culturing macadimia nut seedlings, applying nutrient solution to a planting matrix every week, and simultaneously applying growth promoting solution to the leaf surfaces of the macadimia nut seedlings; the growth promoting liquid contains cysteine, lysine and proline. The technical scheme can solve the technical problem that the grafting success rate is not high due to limited activity of the seedling stock seedling in the prior art. The macadimia nut seedling cultivated by the scheme can be used as a macadimia nut seedling stock seedling, and has the advantages of high chlorophyll content, high light energy utilization efficiency, strong mineral element accumulation capacity and the like, can improve the grafting survival rate, promote the seedling growth, shorten the seedling period and have wide application prospect.
Description
Technical Field
The invention relates to the technical field of macadimia nut planting, and particularly relates to a macadimia nut fruit tree seedling raising method.
Background
Macadamia nuts are also called macadamia nut, macadamia walnut and Kunlun nut, and belong to the eye of the dragon, the macadamia nuts belong to evergreen arbor fruit trees. Macadamia is native to the coastal subtropical rainforest in the southeast of queensland, australia and in the north of new south wale. Macadamia nut is rich in unsaturated fatty acid, protein, various vitamins, amino acid and the like, is delicious and tasty, is rich in nutrition and has the reputation of nut king. The history of artificial plantation of macadamia nuts in foreign countries has more than 150 years, the history of introducing macadamia nuts in China has nearly one hundred years, and the commercial cultivation begins in 80 years of the 20 th century.
The field planting of the macadimia nut seedlings needs 8-10 years to bear fruit; the grafted seedlings bear fruits 3-4 years earlier than the seedlings by 5-6 years. The conventional seedling raising and grafting is crisp and hard, gum quickly flows out after being injured to form an isolation layer between the stock and the spike, and the stock and the spike are difficult to graft and survive. Therefore, macadamia nut seedling stock seedlings are generally adopted for grafting, so that the grafting survival rate is improved, the seedling growth is promoted, and the seedling culture period is shortened. How to simply, rapidly and efficiently culture the seedling stock seedling with high activity is the key for improving the economic benefit of the macadamia nut fruit tree and the nut quality.
Disclosure of Invention
The invention aims to provide a seedling raising method for macadimia nut fruit trees, and aims to solve the technical problem that grafting success rate is low due to limited activity of seedling stock seedlings in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a seedling raising method for macadimia nut trees comprises the steps of placing a microbial agent in a planting matrix, and then fixing the value of macadimia nut seedlings in the planting matrix; culturing macadimia nut seedlings, applying nutrient solution to a planting matrix every week, and simultaneously applying growth promoting solution to the leaf surfaces of the macadimia nut seedlings; the growth promoting liquid contains cysteine, lysine and proline.
The principle and the advantages of the scheme are as follows: the seedling raising method of the scheme can obviously improve the chlorophyll content of the seedling (seedling stock seedling), the chlorophyll a and b contents are increased, and the ratio of the chlorophyll a to the chlorophyll b is increased, so that the photosynthetic efficiency of the plant is ensured, and the quality of the seedling is greatly improved. The macadimia nut seedlings cultivated by the scheme can be used as the macadimia nut seedling stock seedlings, and are grafted on the macadimia nut adult trees, so that the grafting survival rate can be improved, the seedling growth can be promoted, and the seedling cultivation period can be shortened. The inventor tests a large number of different amino acids, and finds that the combination of three amino acids, namely cysteine, lysine and proline, has a synergistic growth promoting effect on the growth of macadamia nut seedlings. The inventor tries to replace lysine with arginine, replace proline with histidine and replace cysteine with aspartic acid, so that the growth promoting effect on seedlings is reduced, the content of chlorophyll is increased to a level lower than that of the technical scheme, and the ratio of chlorophyll a to chlorophyll b is also reduced. The inventor also tried the growth promoting liquid without proline, the growth promoting liquid without cysteine and the growth promoting liquid without lysine, and the chlorophyll content of the seedling is further reduced. This shows that cysteine, lysine and proline have a certain synergistic effect, and the combined use of the three can effectively improve the growth state of the seedlings.
In conclusion, the seedlings cultured according to the scheme have the advantages of high chlorophyll content, high light energy utilization efficiency, strong mineral element accumulation capacity and the like, and can improve the grafting survival rate, promote the growth of seedlings and shorten the seedling culture period.
Further, the growth promoting solution contains 0.2-0.4mM of cysteine, 0.1-0.2mM of lysine and 0.2-0.4mM of proline.
By adopting the technical scheme, the three amino acids have synergistic effect in the concentration range, so that the accumulation of nitrogen, phosphorus and potassium in plants and the synthesis of chlorophyll are promoted.
Further, the dosage of the growth promoting liquid is 20mL for each macadamia nut seedling.
By adopting the technical scheme, the spraying amount can ensure that various amino acids in the growth promoting liquid play an ideal growth promoting role.
Further, the microbial agent consists of bacillus licheniformis and lactobacillus plantarum.
By adopting the technical scheme, the bacillus licheniformis and the lactobacillus plantarum are synergistic, the accumulation of nitrogen, phosphorus and potassium by plants is promoted, and the synthesis efficiency of various chlorophyll and the accumulation amount of the chlorophyll in seedling leaves are promoted.
Further, the mass ratio of the bacillus licheniformis to the lactobacillus plantarum is 4-8: 1.
By adopting the technical scheme, the bacillus licheniformis and the lactobacillus plantarum in the proportion can exert the most ideal growth promoting effect. If the amount of lactobacillus plantarum is too high, the growth of seedlings is adversely affected, but the addition of lactobacillus plantarum in a proper amount is effective in promoting plant growth.
Further, the amount of the microbial inoculum is 30g per macadamia seedling.
By adopting the technical scheme, 30g of microbial agent is applied to each seedling, so that enough microbes with growth promoting effect can be provided for the seedling, and positive promoting effect on the growth of the seedling is achieved.
Further, the conditions for culturing the macadamia nut seedlings are 85-90% of humidity, 25-27 ℃ of room temperature, 2000-3000lx of illumination intensity, and 12h of continuous illumination is given every day.
By adopting the technical scheme, the macadimia nut seedlings are under the appropriate growth condition so as to improve the growth quality of the macadimia nut plants.
Further, the water content of the planting substrate is maintained at 55-65%.
By adopting the technical scheme, the water content of the planting matrix is a relatively suitable condition for seedling growth.
Further, the nutrient solution is a Hoagland nutrient solution.
By adopting the technical scheme, the Hoagland nutrient solution is a conventional nutrient solution in the prior art, is easy to obtain and can provide sufficient nutrition for plants.
Further, the planting substrate is perlite with the grain size of 30 meshes.
By adopting the technical scheme, the perlite with the particle size can ensure the full penetration and filling of nutrient substances, and provide good nutrient environment and support for the growth of seedlings.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following examples and experimental examples are conventional means well known to those skilled in the art, and the materials, reagents and the like used therein are commercially available.
The following is further detailed by way of specific embodiments:
example 1
Plants which are robust in growth, free of diseases and insect pests, high in yield and stable in yield and good in nut quality are selected as seed trees, and the tree seeds used in the embodiment are Own Choice (OC). The tree species (mature tree) has dense crown, shrub shape, open, naturally drooping branches, small and twisted leaves, no or few thorns on leaf edges, reverse coiling, small and many branches, good wind resistance, high yield and medium and large fruits, and is suitable for seedling culture experiments. Collecting fully mature seeds, selecting fruits with naturally cracked outer shells, spreading the fruits at an indoor ventilation position, and taking out the seeds after cracking. The small seeds were sieved through a sieve with a pore size of 3cm and then surface sterilized with 0.5% potassium permanganate. And sowing seeds in a seedling raising tray, placing sterilized perlite in the seedling raising tray, and watering in time until the seeds germinate and grow into seedlings. To ensure the parallelism of the subsequent experiments, the selection criteria for seedlings were as follows: has 2-3 true leaves; 2-3 roots meeting the condition that the length is more than or equal to 4cm and 8cm are provided, and the seedlings selected for the subsequent experiments have consistent growth vigor.
Selecting 30-mesh perlite as a planting matrix of the seedling, performing damp-heat sterilization on the perlite (121 ℃, 0.1MPa and 1h), then putting the perlite into a planting container, wherein the thickness of the perlite is 20cm, and then putting 30g of microbial inoculum on the upper surface of the perlite. And then placing the seedlings into a planting container, and covering the roots of the seedlings with perlite, wherein the thickness of the covering is about 5 cm. Placing the planting container with planted seedlings into a tissue culture room, keeping the relative air humidity of the tissue culture room at 85-90% and the room temperature at 25-27 ℃, keeping the water content of the planting matrix at 55-65%, and performing water management according to the value of the water content. Continuous illumination was given for 12h each day (illumination intensity 2000-. To ensure that the seedlings are adequately nourished, conventional Hoagland's nutrient solution is applied to the planting medium every week (the Hoagland's nutrient solution is applied for the first time on the day of planting), and 20mL of Hoagland's nutrient solution is added to each seedling. And simultaneously spraying growth promoting liquid to the leaves of the seedlings, and uniformly spraying 20ml of growth promoting liquid to the leaves of each seedling. The growth promoting solution contains 0.2mM of cysteine, 0.1mM of lysine and 0.2mM of proline.
The microbial agent consists of Bacillus licheniformis (Weigmann) Chester, ATCC 14580) and Lactobacillus plantarum (Orla-Jensen) Bergey et al, ATCC 14917). The preparation process of the microbial agent comprises the following steps: conventional strain activation is firstly carried out, bacillus licheniformis is inoculated in an LB solid culture medium, lactobacillus plantarum is inoculated in an MRS solid culture medium, and the strain activation is completed after the strain is placed in a constant temperature incubator and is statically cultured for 24 hours at the temperature of 28 ℃. The LB solid, liquid medium and MRS solid, liquid medium are common media in the prior art, and are not described herein. Inoculating single colony of Bacillus licheniformis in LB liquid culture medium at 30 deg.C and 12 deg.CCulturing at 0rpm for 24h to obtain Bacillus licheniformis primary culture solution; inoculating the lactobacillus plantarum single colony in an MRS liquid culture medium, and performing static culture at 30 ℃ for 24 hours to obtain a lactobacillus plantarum grade culture solution. Then, the bacillus licheniformis grade culture solution was inoculated in LB broth (inoculum size 10 vol.%), and the lactobacillus plantarum grade culture solution was inoculated in MRS broth (inoculum size 10 vol.%). Culturing two kinds of microorganisms to OD600The value is 1, the culture is finished, centrifugation and filtration are carried out to obtain thalli of two bacteria, and the bacillus licheniformis thalli and the lactobacillus plantarum thalli are mixed according to the mass ratio of 4:1 to obtain the microbial agent.
Culturing macadimia nut seedlings for 8 weeks, and carrying out experimental detection on the seedlings after the culture is finished, wherein the experimental detection comprises the measurement of the total nitrogen phosphorus potassium content of plant roots and leaves and the measurement of the chlorophyll content. When the total nitrogen, phosphorus and potassium are measured, a plant material is treated by adopting a sulfuric acid-hydrogen peroxide digestion method, then the nitrogen content is measured by adopting a semi-micro diffusion method, the phosphorus content is measured by adopting a molybdenum-antimony-scandium colorimetric method, and the potassium content is measured by adopting a flame photometer method. The total nitrogen phosphorus potassium is measured by selecting 10 seedlings cultured for 8 weeks, taking leaves (three leaves at the top of each seedling are cleaned and then tested) and roots (all roots are cleaned and then tested) to respectively measure the total nitrogen phosphorus potassium, and the test results are expressed in percentage by mass in table 1. Measuring the content of chlorophyll by a conventional ethanol extraction method, extracting chlorophyll of plant leaves by using 80% ethanol, measuring the content of chlorophyll by a conventional spectrophotometry method, respectively measuring the absorbance photometric values at 663nm and 645nm, and calculating the content of chlorophyll a and chlorophyll b (shown in the form that the chlorophyll accounts for the mass percent of the fresh weight of the leaves) and the ratio of the chlorophyll a to the chlorophyll b. Detection of chlorophyll 10 seedlings cultured for 8 weeks were selected, and chlorophyll detection was performed by taking leaves (three leaves at the top of each seedling were washed and chlorophyll extraction was performed), and the experimental results are detailed in table 2.
The macadimia nut seedlings cultivated in the method can be used as macadimia nut seedling stock seedlings, and can be grafted on macadimia nuts to form trees. The seedling cultured according to the scheme has the advantages of high chlorophyll content, high light energy utilization efficiency, strong mineral element accumulation capacity and the like, and can improve the grafting survival rate, promote the growth of the seedling and shorten the seedling culture period.
Example 2
This example is basically the same as example 1 except that the composition of the growth-promoting solution was 0.4mM for cysteine, 0.3mM for lysine, and 0.4mM for proline; mixing the bacillus licheniformis thalli and the lactobacillus plantarum thalli according to the mass ratio of 8:1 to obtain the microbial agent.
Comparative example 1
This comparative example is substantially the same as example 1 except that the composition of the growth promoting solution was 0.2mM cysteine, 0.1mM arginine and 0.2mM proline.
Comparative example 2
This comparative example is substantially the same as example 1 except that the composition of the growth-promoting solution was 0.2mM cysteine, 0.1mM lysine and 0.2mM histidine.
Comparative example 3
This comparative example is substantially the same as example 1 except that the composition of the growth promoting solution was 0.2mM aspartic acid, 0.1mM lysine and 0.2mM proline.
Comparative example 4
This comparative example is substantially the same as example 1 except that the composition of the growth-promoting solution was 0.2mM cysteine and 0.1mM lysine.
Comparative example 5
This comparative example is substantially the same as example 1 except that the composition of the growth promoting solution was 0.1mM of lysine and 0.2mM of proline.
Comparative example 6
This comparative example is substantially the same as example 1 except that the composition of the growth-promoting solution was 0.2mM cysteine and 0.2mM proline.
Comparative example 7
This comparative example is basically the same as example 1, except that the three amino acids were dissolved in the Hoagland's nutrient solution and applied to the seedling by root application, without spraying the growth promoting solution onto the leaf surface of the seedling. The concentrations of cysteine, lysine and proline in the Hoagland's nutrient solution were 0.2mM, 0.1mM and 0.2 mM.
Comparative example 8
This comparative example is substantially the same as example 1, except that the microbial agent contains only Bacillus licheniformis.
Comparative example 9
This comparative example is basically the same as example 1, except that the microbial agent contains only Lactobacillus plantarum.
Comparative example 10
This comparative example is substantially the same as example 1 except that a microbial inoculum was not added to the perlite.
Comparative example 11
This comparative example is substantially the same as example 1, except that the foliage was sprayed without using a lift-promoting agent.
The experimental results of example 1/2 and comparative examples 1-11 are shown in tables 1 and 2, and 10 specimens (one seedling) were selected for each test in each example.
Table 1: nitrogen phosphorus potassium content test results (mean + -SE, n ═ 10)
Table 1 Using Duncan multiple alignment analysis, the lower case letters in the same column indicate significant differences (p < 0.05).
The experimental results in table 1 show that the embodiment adopts the microbial agent and the means of spraying the promotion solution on the leaf surface, so that the contents of nitrogen, phosphorus, potassium and the like of the plant can be improved. Compared with the method of using the promotion solution alone in the comparative example 10 and the method of using the microbial agent alone in the comparative example 11, the two methods are combined, so that the accumulation of the seedling on nutrient elements can be effectively promoted, the grafting survival rate can be improved, the seedling growth can be promoted, and the seedling culture period can be shortened after grafting. In comparative example 9, the lactobacillus plantarum alone did not promote the accumulation of npk (relative to comparative example 10), but rather significantly reduced the capacity of the plant to accumulate nitrogen. However, the combined use of lactobacillus plantarum and bacillus licheniformis (examples 1 and 2) can improve the accumulation of nitrogen, phosphorus and potassium of plants, and a synergistic effect is formed.
Table 2; chlorophyll content test result (mean ± SE, n ═ 10)
| Grouping | Chlorophyll a (% fresh weight) | Chlorophyll b (% fresh weight) | Chlorophyll a/chlorophyll b |
| Example 1 | 1.957±0.039a | 1.067±0.017c | 1.83 |
| Example 2 | 1.857±0.022a | 0.991±0.012c | 1.87 |
| Comparative example 1 | 1.621±0.038b | 0.934±0.010a | 1.74 |
| Comparative example 2 | 1.729±0.048b | 0.983±0.017c | 1.76 |
| Comparative example 3 | 1.683±0.037b | 0.957±0.021a | 1.76 |
| Comparative example 4 | 1.467±0.051c | 0.887±0.016b | 1.65 |
| Comparative example 5 | 1.436±0.067c | 0.894±0.015b | 1.61 |
| Comparative example 6 | 1.511±0.077c | 0.912±0.021a | 1.66 |
| Comparative example 7 | 1.722±0.049b | 0.943±0.029a | 1.83 |
| Comparative example 8 | 1.576±0.065d | 0.873±0.030b | 1.81 |
| Comparative example 9 | 1.589±0.059d | 0.889±0.018b | 1.79 |
| Comparative example 10 | 1.247±0.034e | 0.943±0.024a | 1.32 |
| Comparative example 11 | 1.171±0.011e | 0.846±0.032b | 1.38 |
Table 2 Using Duncan multiple alignment analysis, the lower case letters in the same column indicate significant differences (p < 0.05).
As can be seen from the data in table 2, in the embodiments 1 and 2, the seedling raising method according to the present disclosure can significantly improve the chlorophyll content of the seedling (seedling stock seedling), the chlorophyll a and b contents are both increased, and the ratio of the chlorophyll a to the chlorophyll b is also increased, so as to ensure the photosynthetic efficiency of the plant and greatly improve the growth effect of the seedling. The macadimia nut seedlings cultivated by the method can be used as macadimia nut seedling stock seedlings, and can be grafted on the macadimia nut adult trees, so that the grafting survival rate can be improved, the seedling growth can be promoted, and the seedling cultivation period can be shortened.
The effect of arginine for lysine in comparative example 1, histidine for proline in comparative example 2, and aspartic acid for cysteine in comparative example 3 was reduced, the chlorophyll content was increased to a level lower than that in the examples, and the ratio of chlorophyll a to chlorophyll b was also reduced. Comparative example 4 did not use proline, comparative example 5 did not use cysteine, and comparative example 6 did not use lysine, and the chlorophyll content of seedlings was further reduced. This shows that cysteine, lysine and proline have a certain synergistic effect, and the combined use of the three can effectively improve the growth state of the seedlings.
Comparative example 7 the method of spraying the growth promoting liquid to the leaf surface was changed to root application, but the effect of promoting photosynthesis of seedlings was inferior to that of the example, which shows that the application method of the growth promoting agent has a significant effect on the effect, and the leaf surface spraying is preferable. Comparative examples 8 and 9 respectively use bacillus licheniformis and lactobacillus plantarum as microbial agents, but the effect of promoting the chlorophyll content is not ideal, which indicates that the two microbial agents are required to be used together to obtain the ideal growth promoting effect.
The comparison example 10 does not use the microbial inoculum, the comparison example 11 does not use the growth promoting liquid, and the chlorophyll content in the seedling, the ratio of chlorophyll a to chlorophyll b are greatly different from those of the embodiment, which shows that the microbial inoculum and the growth promoting liquid need to be used simultaneously to effectively promote the synthesis and accumulation of chlorophyll of the seedling, thereby having positive influence on the photosynthesis efficiency of the plant.
The foregoing is merely an example of the present invention and common general knowledge in the art of designing and/or characterizing particular aspects and/or features is not described in any greater detail herein. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several variations and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A seedling raising method for macadimia nut trees is characterized in that a microbial agent is placed in a planting matrix, and then macadimia nut seedlings are placed in the planting matrix in a fixed value mode; culturing macadimia nut seedlings, applying nutrient solution to a planting matrix every week, and simultaneously applying growth promoting solution to the leaf surfaces of the macadimia nut seedlings; the growth promoting liquid contains cysteine, lysine and proline.
2. The method of claim 1, wherein the growth promoting solution comprises 0.2-0.4mM cysteine, 0.1-0.2mM lysine and 0.2-0.4mM proline.
3. The method for raising seedlings of macadamia nut trees as claimed in claim 2, wherein the amount of the growth promoting liquid is 20mL per seedling of the macadamia nut sprayed.
4. The method of claim 3, wherein the microbial inoculum comprises Bacillus licheniformis and Lactobacillus plantarum.
5. The method for raising seedlings of macadamia nut trees according to claim 4, wherein the mass ratio of the bacillus licheniformis to the lactobacillus plantarum is 4-8: 1.
6. The method of claim 5, wherein the amount of the microbial inoculum is 30g per seedling of macadamia nut.
7. The method as claimed in claim 6, wherein the conditions for culturing the seedlings of macadamia nut are humidity 85-90%, room temperature 25-27 ℃, illumination intensity 2000-.
8. The method of claim 5, wherein the moisture content of the planting substrate is maintained at 55-65%.
9. The method of claim 8, wherein the nutrient solution is Hoagland's nutrient solution.
10. The method of claim 9, wherein the planting substrate is perlite with a particle size of 30 meshes.
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