CN114402984B - Seedling raising method for macadimia nut fruit trees - Google Patents
Seedling raising method for macadimia nut fruit trees Download PDFInfo
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- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000004472 Lysine Substances 0.000 claims abstract description 19
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- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims abstract description 18
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- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims abstract description 7
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 24
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- 229930002868 chlorophyll a Natural products 0.000 description 11
- 229930002869 chlorophyll b Natural products 0.000 description 11
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 11
- 229910052757 nitrogen Inorganic materials 0.000 description 11
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- 235000003704 aspartic acid Nutrition 0.000 description 3
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- 230000015572 biosynthetic process Effects 0.000 description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 3
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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- 241000238631 Hexapoda Species 0.000 description 1
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- XEMZLVDIUVCKGL-UHFFFAOYSA-N hydrogen peroxide;sulfuric acid Chemical compound OO.OS(O)(=O)=O XEMZLVDIUVCKGL-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application relates to the technical field of macadimia nut planting, in particular to a method for growing seedlings of a fruit tree of a macadimia nut, which comprises the following steps: placing a microbial agent in a planting matrix, and setting the value of the macadimia nut seedlings in the planting matrix; culturing macadimia nut seedlings, applying nutrient solution to a planting matrix every week, and simultaneously applying growth promoting solution to leaf surfaces of the macadimia nut seedlings; the growth promoting liquid contains cysteine, lysine and proline. The technical scheme can solve the technical problem that the grafting success rate is not high due to limited activity of the seedling of the stock in the prior art. The macadimia nut seedlings cultivated by the scheme can be used as macadimia nut actual stock seedlings, and the seedlings have the advantages of high chlorophyll content, high light energy utilization efficiency, strong mineral element accumulation capacity and the like, can improve grafting survival rate, promote seedling growth, shorten seedling growing period and have wide application prospects.
Description
Technical Field
The application relates to the technical field of macadimia nut planting, in particular to a method for growing seedlings of a fruit tree of a macadimia nut.
Background
Macadamia nuts are also called macadamia nuts, macadamia walnuts and quebracho nuts, and belong to the category of evergreen arbor trees in the genus of the macadamia in the family of the mountain longles. The macadamia nuts originally produced coastal subtropical rain forest in southeast of the state of australia and in north of the state of new south wilt. Macadimia nut is rich in unsaturated fatty acid, protein, multiple vitamins, amino acids and the like, delicious, rich in nutrition and has the reputation of 'king nut'. The foreign artificial planting of macadimia nuts has been 150 years old, the introduction of macadimia nuts in China has been hundreds of years old, and commercial cultivation begins in the 80 th century.
The field planting of the macadimia nut seedlings can be achieved in 8-10 years; the grafted seedling is fruiting 3-4 years earlier than the seedling by 5-6 years. Conventional seedling grafting is crisp and hard, and after injury, the gum flows out quickly to form an isolation layer between the stock ears, so that grafting survival is difficult. Therefore, the macadimia nut seedling stock seedlings are generally used for grafting, so that the grafting survival rate is improved, the growth of the seedlings is promoted, and the seedling period is shortened. How to simply, quickly and efficiently culture the high-activity seedling of the stock is a key for improving the economic benefit and the quality of the macadimia nut.
Disclosure of Invention
The application aims to provide a method for growing seedlings of macadimia nut fruit trees, which aims to solve the technical problem that grafting success rate is low due to limited activity of actual stock seedlings in the prior art.
In order to achieve the above purpose, the application adopts the following technical scheme:
a method for culturing seedlings of macadimia nut fruit trees comprises the steps of placing a microbial agent in a planting matrix, and setting the value of the macadimia nut seedlings in the planting matrix; culturing macadimia nut seedlings, applying nutrient solution to a planting matrix every week, and simultaneously applying growth promoting solution to leaf surfaces of the macadimia nut seedlings; the growth promoting liquid contains cysteine, lysine and proline.
The principle and the advantages of the scheme are as follows: the seedling raising method can have a remarkable improvement effect on the chlorophyll content of seedlings (stock seedlings), the content of chlorophyll a and the content of chlorophyll b are increased, the ratio of chlorophyll a to chlorophyll b are increased, the photosynthetic efficiency of plants is further guaranteed, and the quality of the seedlings is improved greatly. The macadimia nut seedlings cultivated by the scheme can be used as the macadimia nut real-growth stock seedlings, and can be grafted on the macadimia nut adult tree, so that the grafting survival rate can be improved, the growth of seedlings can be promoted, and the seedling raising period can be shortened. The inventor tests a large number of different amino acids, and discovers that the combination of three amino acids of cysteine, lysine and proline produces synergistic growth promotion effect on the growth of macadimia nut seedlings. The inventor tries to replace lysine with arginine, replace proline with histidine and replace cysteine with aspartic acid, so that the promotion effect of seedlings is reduced, the content of chlorophyll is increased to a level lower than that of the technical scheme, and the ratio of chlorophyll a to chlorophyll b is reduced. The inventors also tried to further decrease the chlorophyll content of seedlings without using proline growth promoting liquid, without using cysteine growth promoting liquid, and without using lysine growth promoting liquid. This shows that there is a certain synergistic effect among cysteine, lysine and proline, and the three can be used in combination to effectively promote the growth state of seedlings.
In conclusion, the seedlings cultured according to the scheme have the advantages of high chlorophyll content, high light energy utilization efficiency, high mineral element accumulation capacity and the like, and can improve grafting survival rate, promote seedling growth and shorten seedling culture period.
Further, the growth promoting liquid contains 0.2-0.4mM cysteine, 0.1-0.2mM lysine and 0.2-0.4mM proline.
By adopting the technical scheme, in the concentration range, three amino acids act cooperatively to promote accumulation of nitrogen, phosphorus and potassium in plants and synthesis of chlorophyll.
Further, the dosage of the growth promoting liquid is 20mL for each macadimia nut seedling sprayed.
By adopting the technical scheme, the spraying amount can ensure that various amino acids in the growth promoting liquid have ideal growth promoting effect.
Further, the microbial agent consists of bacillus licheniformis and lactobacillus plantarum.
By adopting the technical scheme, the bacillus licheniformis and the lactobacillus plantarum are synergistic, so that the accumulation of plants on nitrogen, phosphorus and potassium is improved, and the synthesis efficiency of various chlorophyll and the accumulation amount of the chlorophyll in seedling leaves are improved.
Further, the mass ratio of the bacillus licheniformis to the lactobacillus plantarum is 4-8:1.
By adopting the technical scheme, the bacillus licheniformis and the lactobacillus plantarum with the proportion can exert the most ideal growth promoting effect. If the amount of Lactobacillus plantarum is too high, it will have a negative effect on the growth of seedlings, but the addition of Lactobacillus plantarum in an appropriate amount is effective to promote plant growth.
Further, the microbial agent was used in an amount of 30g per macadamia nut seedling.
By adopting the technical scheme, 30g of microbial agent is applied to each seedling, so that enough microorganisms with growth promoting effect can be provided for the seedling, and the forward promoting effect is achieved on the growth of the seedling.
Further, the condition for culturing macadimia nut seedlings is that the humidity is 85-90%, the room temperature is 25-27 ℃, the illumination intensity is 2000-3000lx, and continuous illumination is given for 12 hours every day.
By adopting the technical scheme, the macadimia nut seedlings are under proper growth conditions so as to improve the plant growth quality.
Further, the water content of the planting substrate is maintained at 55-65%.
By adopting the technical scheme, the water content of the planting matrix is a condition suitable for seedling growth.
Further, the nutrient solution is Hoagland's nutrient solution.
By adopting the technical scheme, the Hoagland nutrient solution is a conventional nutrient solution in the prior art, is easy to obtain and can provide sufficient nutrition for plants.
Further, the planting matrix is perlite with the particle size of 30 meshes.
By adopting the technical scheme, the perlite with the particle size can ensure the full permeation and filling of nutrient substances, and provide good nutrient environment and support for the growth of seedlings.
Detailed Description
The present application will be described in further detail with reference to examples, but embodiments of the present application are not limited thereto. Unless otherwise indicated, the technical means used in the following examples and experimental examples are conventional means well known to those skilled in the art, and the materials, reagents and the like used are all commercially available.
The following is a further detailed description of the embodiments:
example 1
Plants which are strong in growth, free of plant diseases and insect pests, high in yield and stable in yield and good in nut quality are selected as seed picking trees, and the tree seeds used in the embodiment are grown choice (OC). The tree is dense in crown, shrub-shaped, open, natural in drooping of branches, small in leaf distortion, free of thorn or little thorn at leaf edges, small and more in branches, good in wind resistance, high in yield, medium in fruits and large in size, and is suitable for seedling experiments. Collecting fully mature seeds, selecting fruits with naturally cracked outer seed shells, spreading the fruits at indoor ventilation positions, and taking out the seeds after cracking. The small seeds were removed by sieving with a sieve having a pore size of 3cm, and then surface-sterilized with 0.5% potassium permanganate. Sowing seeds in a seedling raising tray, placing sterilized perlite in the seedling raising tray, and watering in time until the seeds germinate and grow into seedlings. To ensure parallelism in subsequent experiments, the selection criteria for seedlings were as follows: 2-3 true leaves; 2-3 roots meeting the condition that the length of 8cm is more than or equal to 4cm are provided, and seedlings selected for subsequent experiments are consistent in growth vigor.
The method comprises the steps of selecting 30-mesh perlite as a planting matrix of seedlings, carrying out damp-heat sterilization (121 ℃ C., 0.1MPa, 1 h) on the perlite, then placing the perlite into a planting container, wherein the thickness of the perlite is 20cm, and then placing 30g of microbial agent on the upper surface of the perlite. Then placing the seedlings into a planting container, and covering the roots of the seedlings by perlite, wherein the covering thickness is about 5cm. Placing the planting container of the planted seedlings into a tissue culture room, keeping the relative humidity of air in the tissue culture room to be 85-90%, keeping the room temperature to be 25-27 ℃, keeping the water content of the planting matrix to be 55-65%, and carrying out water management according to the water content value. Continuous 12h of light (intensity of light 2000-3000 lx) was given daily. To ensure adequate nutrition for the seedlings, conventional Hoagland's nutrient solution (applied first on the day of just-fixed planting) was applied weekly to the planting substrate, and 20mL of Hoagland's nutrient solution was added to each seedling. And simultaneously spraying growth promoting liquid to the leaves of the seedlings, and uniformly spraying 20ml of growth promoting liquid to the leaf surfaces of each seedling. The growth-promoting solution contained 0.2mM cysteine, 0.1mM lysine and 0.2mM proline.
The microbial inoculum consisted of bacillus licheniformis (Bacillus licheniformis (Weigmann) cheter, ATCC 14580) and lactobacillus plantarum (Lactobacillus plantarum subsp. The preparation process of the microbial agent comprises the following steps: firstly, conventional strain activation is carried out, bacillus licheniformis is inoculated in an LB solid culture medium, lactobacillus plantarum is inoculated in an MRS solid culture medium, and the strain is subjected to stationary culture for 24 hours at 28 ℃ in a constant temperature incubator, so that the strain activation is completed. LB solid and liquid culture media and MRS solid and liquid culture media are common culture media in the prior art, and are not described herein. Inoculating single bacterial colony of bacillus licheniformis into LB liquid culture medium, culturing at 30deg.C and 120rpm for 24h to obtain primary bacillus licheniformis culture solution; inoculating the single colony of the lactobacillus plantarum into an MRS liquid culture medium, and standing and culturing for 24 hours at the temperature of 30 ℃ to obtain a lactobacillus plantarum grade culture solution. Then, the Bacillus licheniformis grade culture solution was inoculated into LB liquid medium (inoculum size 10 vol.%) and the Lactobacillus plantarum grade culture solution was inoculated into MRS liquid medium (inoculum size 10 vol.%). Two microorganisms were cultured to OD 600 The value is 1, the culture is finished, the two bacteria are obtained by centrifugation and filtration, and the bacillus licheniformis bacteria and the lactobacillus plantarum bacteria are mixed according to the mass ratio of 4:1, so that the microbial agent of the scheme is obtained.
Culturing macadimia nut seedlings lasts for 8 weeks, and after culturing, experimental detection is carried out on the seedlings, wherein the experimental detection comprises determination of the content of total nitrogen, phosphorus and potassium in plant roots and leaves and determination of chlorophyll content. When the determination of total nitrogen, phosphorus and potassium is carried out, firstly, a sulfuric acid-hydrogen peroxide digestion method is adopted to treat plant materials, then a semi-micro diffusion method is adopted to determine the nitrogen content, a molybdenum-antimony-scandium colorimetric method is adopted to determine the phosphorus content, and a flame photometer method is adopted to determine the potassium content. 10 seedlings after 8 weeks of culture are selected for measuring the total nitrogen, phosphorus and potassium, the leaves (three leaves at the top of each seedling are cleaned and tested) and the roots (all roots of each seedling are cleaned and tested) are respectively measured, the total nitrogen, phosphorus and potassium are expressed in terms of mass percent, and the experimental results are shown in Table 1 in detail. The chlorophyll content is determined by a conventional ethanol extraction method, chlorophyll in plant leaves is extracted by using 80% ethanol, then the chlorophyll content is determined by a conventional spectrophotometry method, absorbance values at 663nm and 645nm are respectively determined, and then the chlorophyll a and chlorophyll b contents (both shown in the form of mass percent of chlorophyll in fresh weight of leaves) and the ratio of chlorophyll a to chlorophyll b are calculated. Chlorophyll detection 10 seedlings were selected after 8 weeks of cultivation, and leaves were taken (three leaves at the top of each seedling were washed and chlorophyll was extracted) for chlorophyll detection, and the experimental results are shown in table 2.
The macadimia nut seedlings cultivated in the embodiment can be used as macadimia nut real stock seedlings, and can be grafted on macadimia nut adult trees. The seedlings cultured according to the scheme have the advantages of high chlorophyll content, high light energy utilization efficiency, strong mineral element accumulation capacity and the like, and can improve grafting survival rate, promote seedling growth and shorten seedling period.
Example 2
This example is substantially the same as example 1, except that the growth promoting liquid has a composition of cysteine 0.4mM, lysine 0.3mM and proline 0.4mM; the bacillus licheniformis thalli and the lactobacillus plantarum thalli are mixed according to the mass ratio of 8:1, so that the microbial agent of the scheme is obtained.
Comparative example 1
This comparative example is substantially the same as in example 1, except that the composition of the growth promoting fluid is 0.2mM cysteine, 0.1mM arginine and 0.2mM proline.
Comparative example 2
This comparative example is substantially the same as in example 1, except that the growth promoting fluid has a composition of 0.2mM cysteine, 0.1mM lysine and 0.2mM histidine.
Comparative example 3
This comparative example is substantially the same as in example 1, except that the growth promoting fluid has a composition of 0.2mM aspartic acid, 0.1mM lysine and 0.2mM proline.
Comparative example 4
This comparative example is substantially the same as in example 1, except that the growth promoting fluid has a composition of 0.2mM cysteine and 0.1mM lysine.
Comparative example 5
This comparative example is substantially the same as in example 1, except that the growth promoting fluid has a composition of 0.1mM lysine and 0.2mM proline.
Comparative example 6
This comparative example is substantially the same as in example 1, except that the growth promoting fluid has a composition of 0.2mM cysteine and 0.2mM proline.
Comparative example 7
This comparative example is basically the same as example 1 except that the growth promoting liquid is not sprayed on the leaf surfaces of the seedlings, but three amino acids are dissolved in the Hoagland's nutrient solution and applied to the seedlings by the root application method. The concentrations of cysteine, lysine and proline in the Hoagland's nutrient solution were 0.2mM, 0.1mM and 0.2mM.
Comparative example 8
This comparative example is substantially the same as example 1 except that the microbial agent contains only Bacillus licheniformis.
Comparative example 9
This comparative example is basically the same as example 1 except that the microbial agent contains only Lactobacillus plantarum.
Comparative example 10
This comparative example is essentially the same as example 1, except that no microbial agent is added to the perlite.
Comparative example 11
This comparative example is essentially the same as example 1, except that the foliar spray is not applied with an elevating agent.
The results of experiments in examples 1/2 and comparative examples 1 to 11 are shown in tables 1 and 2, and 10 samples (one sample is a seedling) were taken for each test in each example.
Table 1: results of the nitrogen phosphorus potassium content test (mean±se, n=10)
Table 1 using a danken multiplex comparison analysis, the lower case letter differences in the same column indicate significant differences (p < 0.05).
From the experimental results in table 1, the embodiment adopts the means of microbial agent and foliage spraying promoting liquid, so that the contents of nitrogen, phosphorus, potassium and the like of plants can be improved. Compared with the method of singly using the lifting promoting liquid in the comparative example 10 and the method of singly using the microbial agent in the comparative example 11, the two methods are combined, so that the accumulation of nutrient elements of seedlings can be effectively promoted, the survival rate of grafting can be improved, the growth of seedlings can be promoted, and the seedling period can be shortened after grafting. In comparative example 9, lactobacillus plantarum alone did not promote accumulation of nitrogen, phosphorus and potassium (relative to comparative example 10), but rather significantly reduced the ability of the plant to accumulate nitrogen. However, the combination of Lactobacillus plantarum and Bacillus licheniformis (examples 1 and 2) can enhance the accumulation of nitrogen, phosphorus and potassium in plants, and form a synergistic effect.
Table 2; chlorophyll content test results (mean±se, n=10)
| Grouping | Chlorophyll a (% fresh weight) | Chlorophyll b (% fresh weight) | Chlorophyll a/chlorophyll b |
| Example 1 | 1.957±0.039a | 1.067±0.017c | 1.83 |
| Example 2 | 1.857±0.022a | 0.991±0.012c | 1.87 |
| Comparative example 1 | 1.621±0.038b | 0.934±0.010a | 1.74 |
| Comparative example 2 | 1.729±0.048b | 0.983±0.017c | 1.76 |
| Comparative example 3 | 1.683±0.037b | 0.957±0.021a | 1.76 |
| Comparative example 4 | 1.467±0.051c | 0.887±0.016b | 1.65 |
| Comparative example 5 | 1.436±0.067c | 0.894±0.015b | 1.61 |
| Comparative example 6 | 1.511±0.077c | 0.912±0.021a | 1.66 |
| Comparative example 7 | 1.722±0.049b | 0.943±0.029a | 1.83 |
| Comparative example 8 | 1.576±0.065d | 0.873±0.030b | 1.81 |
| Comparative example 9 | 1.589±0.059d | 0.889±0.018b | 1.79 |
| Comparative example 10 | 1.247±0.034e | 0.943±0.024a | 1.32 |
| Comparative example 11 | 1.171±0.011e | 0.846±0.032b | 1.38 |
Table 2 shows that there is a significant difference (p < 0.05) in the lowercase letters of the same column using Duncan multiple comparison analysis.
From the data in table 2, it can be seen that in examples 1 and 2, the seedling raising method according to the present embodiment can have a significant improvement effect on the chlorophyll content of seedlings (actual stock seedlings), the chlorophyll a and b contents are both increased, and the ratio of chlorophyll a to chlorophyll b is also increased accordingly, thereby ensuring the photosynthetic efficiency of plants, and greatly improving the growth effect of seedlings. The macadimia nut seedlings cultivated by the method can be used as the macadimia nut actual stock seedlings, and the macadimia nut seedlings can be grafted on the macadimia nut adult tree, so that the grafting survival rate can be improved, the growth of seedlings can be promoted, and the seedling raising period can be shortened.
Arginine was used instead of lysine in comparative example 1, histidine was used instead of proline in comparative example 2, aspartic acid was used instead of cysteine in comparative example 3, the effect of promoting growth of seedlings was reduced, the level of chlorophyll was not higher than in examples and the ratio of chlorophyll a to chlorophyll b was also reduced. Comparative example 4 did not use proline, comparative example 5 did not use cysteine, comparative example 6 did not use lysine, and chlorophyll content of seedlings was further decreased. This shows that there is a certain synergistic effect among cysteine, lysine and proline, and the three can be used in combination to effectively promote the growth state of seedlings.
The comparative example 7 changes the mode of spraying the growth promoting liquid on the leaf surface into root application, but the effect of promoting the photosynthesis of seedlings is poorer than that of the example, which shows that the application mode of the growth promoting agent has more obvious influence on the effect, and the leaf surface spraying is preferable. Comparative examples 8 and 9 used Bacillus licheniformis and Lactobacillus plantarum, respectively, as microbial agents, but the effect of promoting chlorophyll content was not ideal, indicating that the combination of these two agents was required to achieve the desired growth promoting effect.
The comparative example 10 uses no microbial agent, the comparative example 11 uses no growth promoting liquid, and the chlorophyll content in seedlings and the ratio of chlorophyll a to chlorophyll b are greatly different from those of the examples, which indicates that the microbial agent and the growth promoting liquid need to be used simultaneously to effectively promote the synthesis and accumulation of chlorophyll in the seedlings, and further positively influence the photosynthesis efficiency of plants.
The foregoing is merely exemplary of the present application, and specific technical solutions and/or features that are well known in the art have not been described in detail herein. It should be noted that, for those skilled in the art, several variations and modifications can be made without departing from the technical solution of the present application, and these should also be regarded as the protection scope of the present application, which does not affect the effect of the implementation of the present application and the practical applicability of the patent. The protection scope of the present application is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (1)
1. A method for culturing seedlings of macadimia nut fruit trees is characterized in that a microbial agent is placed in a planting matrix, and then the macadimia nut seedlings are planted in the planting matrix; culturing macadimia nut seedlings, applying nutrient solution to a planting matrix every week, and simultaneously applying growth promoting solution to leaf surfaces of the macadimia nut seedlings; the growth promoting liquid contains cysteine, lysine and proline;
the growth promoting liquid contains 0.2-0.4mM cysteine, 0.1-0.2mM lysine and 0.2-0.4mM proline; the dosage of the growth promoting liquid is 20mL of each macadimia nut seedling sprayed each time;
the microbial agent consists of bacillus licheniformis and lactobacillus plantarum; the mass ratio of the bacillus licheniformis to the lactobacillus plantarum is 8:1; the microbial agent is applied in an amount of 30g per macadimia nut seedling; the microbial agent is prepared by the following method: inoculating single bacterial colony of bacillus licheniformis into LB liquid culture medium, culturing at 30deg.C and 120rpm for 24h to obtain primary bacillus licheniformis culture solution; inoculating single colony of lactobacillus plantarum into MRS liquid culture medium, and standing and culturing at 30 ℃ for 24 hours to obtain primary lactobacillus plantarum culture solution; then, inoculating the bacillus licheniformis primary culture solution into an LB liquid culture medium, and inoculating the lactobacillus plantarum primary culture solution into an MRS liquid culture medium; two microorganisms were cultured to OD 600 The value is 1, the culture is finished, the two bacteria are obtained by centrifugation and filtration, and the bacillus licheniformis bacteria and the lactobacillus plantarum bacteria are mixed to obtain the microbial inoculum;
the nutrient solution is Hoagland's nutrient solution;
culturing macadimia nut seedlings under the conditions of humidity of 85-90%, room temperature of 25-27 ℃ and illumination intensity of 2000-3000lx, and continuously illuminating for 12 hours every day;
the water content of the planting matrix is maintained at 55-65%; the planting matrix is perlite with the particle size of 30 meshes; the thickness of the perlite is 20cm, and then 30g of microbial agent is put on the upper surface of the perlite; next, the seedlings were placed in a planting container, and then the roots of the seedlings were covered with perlite to a thickness of 5cm.
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