CN108530206A - A kind of tea tree foliage bacterial fertilizer and the preparation method and application thereof - Google Patents
A kind of tea tree foliage bacterial fertilizer and the preparation method and application thereof Download PDFInfo
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- CN108530206A CN108530206A CN201810703791.2A CN201810703791A CN108530206A CN 108530206 A CN108530206 A CN 108530206A CN 201810703791 A CN201810703791 A CN 201810703791A CN 108530206 A CN108530206 A CN 108530206A
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- fertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of tea tree foliage bacterial fertilizers and the preparation method and application thereof, with 20 40 parts of Bacillus cercus microbial inoculum, 28 parts of chitosan, 8 12 parts of amino acid, fertile 10 20 parts micro-, 20 40 parts of water.Enhance tea tree disease resistance applied to the yield, improvement tea tree quality, induction for increasing tea tree, promote low age and the growth etc. for the tea tree plant that grows into forest.Have many advantages, such as significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease, improves yield and quality, is of low cost and easy to spread.
Description
Technical field
The present invention relates to tree plant cultivation technologies and fertilising field, and in particular to a kind of tea tree foliage bacterial fertilizer and preparation method thereof
With application.
Background technology
The poor efficiency of chemical fertilizer and its high environmental risk brought are always to perplex the significant problem in the whole world in agricultural production,
Chemical fertilizer use has also resulted in tea place ecological environment severe exacerbation in Tea Production, and tea leaf quality is difficult to ensure.How to make
The special fertilizer of standby tealeaves green ecological, optimizes growth of tea plant environment, improves the yield and quality of tea tree, to promoting entire tea place to give birth to
The improvement of state system is that tea industry sustainable development is of great significance.And the tea leaf quality of simple application of organic fertilizers production is good,
But yield is not high.Although being increased substantially using the tealeaves yield of inorganic fertilizer production merely, quality is declined.And due to tea
Tree is more batches of harvestings in 1 year, relatively continuous and steady to the absorption of nutrient, without obviously peak period.Therefore tea tree
The fertilizer of application should fertilizer efficiency it is long, in order to avoid frequently fertilising cause root system to injure, nutrient supplies stabilization again, to ensure that blade is equal
It is even, improve tea quality.
Foliage bacterial fertilizer by can be absorbed by plant rapidly by plant leaf blade after being sprayed onto plant leaf surface, avoid from
When soil application nutrient easily by soil consolidation, root absorption is slow the problems such as, have that absorb that fast, nutrient loss is few etc. excellent
Point.Currently, there are many kinds of existing foliage bacterial fertilizers, also there are certain effect, but all different journeys in the yield for improving crop
The presence of degree improves the defects of crop yield effect is not notable enough.And there are no the ideals designed exclusively for tea tree in the prior art
Foliage bacterial fertilizer.
Invention content
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, meets tea tree at significant effect
The tea tree foliage bacterial fertilizer of growing nutrient demand.
Technical scheme is as follows：
A kind of tea tree foliage bacterial fertilizer, it is made from the following raw materials in parts by weight：It is prepared from the following ingredients in percentage by group
At：20-40 parts of Bacillus cercus microbial inoculum, 2-8 parts of chitosan, 8-12 parts of amino acid, micro- 10-20 parts of fertilizer, 20-40 parts of water.
Preferably, it is made from the following raw materials in parts by weight：30 parts of Bacillus cercus microbial inoculum, 5 parts of chitosan, amino acid
10 parts, fertile 15 parts micro-, 40 parts of water.
Preferably, the strain contained in Bacillus cercus microbial inoculum is Bacillus cercus LY05 (CCTCC NO:M
2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more).
Wherein, Bacillus cercus microbial inoculum preparation includes the following steps：(1) by bacillus cereus strain LY05, LGY06
Or the inclined-planes WL08 preserve test tube seed, are seeded in minimal medium respectively, shaken cultivation to exponential phase, obtain bacterium
Liquid；(2) bacterium solution of acquisition is seeded to the seed fermentation containing minimal medium with the inoculum concentration of 10% percent by volume
In tank, culture to exponential phase obtains seed liquor；(3) seed liquor of acquisition is connect according to the inoculum concentration of 10% volume ratio
Kind enter fermentation medium in, fermented and cultured 2-3 days at 30-35 DEG C, 150-200rpm, acquisition zymotic fluid, zymotic fluid it is effective
Viable count >=5,000,000,000/mL, zymotic fluid directly dilute 5-20 times as single bacterial strain liquid bacterial agent；(4) by wax-like gemma bar
The single bacterial strain liquid bacterial agent of bacteria strain LY05, LGY06 or WL08 are mixed final by a combination of one or more
Bacillus cercus mix bacterium agent.
Further, the chitosan is water solubility, and deacetylation is more than 95%；The amino acid is proline, sweet ammonia
Acid, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine,
Arbitrary five kinds or more of combination in valine, alanine, serine, arginine, asparatate, cysteine；It is described
Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium of 0.5-1
The mixed fertilizer of+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer；The water is sterile water.
The preparation method of the tea tree foliage bacterial fertilizer of the present invention, comprises the technical steps that：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportioning, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportioning by regulation proportioning；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH6.0-6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to tea tree bacterial fertilizer products.
The bio-bacterial manure of the present invention can be applied in following (1)-(4) are at least one：
(1) increase the yield of tea tree；(2) improve tea tree quality, improve tea tree storage performance；(3) induction enhancing tea tree is anti-
Characteristic of disease, the diseases such as effective prevention and control tea tree anthracnose, moire blight, gall of tea；(4) promote the life of low age and the tea tree plant that grows into forest
It is long.
Compared with prior art, the present invention foliage bacterial fertilizer of the present invention using Bacillus cercus microbial inoculum, plant growth-promoting substance,
The compositions such as a great number of elements and trace element can effectively improve tea tree economic benefit, nutrient and flavour quality, and plant is promoted well to give birth to
It is long, and can effectively induce tea tree plant, the enhancing of fruit disease resistance and prevent tea tree anthracnose, moire blight, gall of tea etc. and is main
The generation of disease is wanted, there is significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease, improve yield and quality, cost
The advantages that cheap and easy to spread.
Specific implementation mode
With reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1：
1. bio-bacterial manure is formed and is prepared：
Bio-bacterial manure A formula compositions and preparation, (1) composition：30 parts of Bacillus cercus microbial inoculum, 5 parts of chitosan, amino acid
10 parts, fertile 15 parts micro-, 40 parts of water.(2) it prepares：1. Bacillus cercus microbial inoculum is taken by regulation proportioning, it is spare；2. being matched by regulation
It takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred to be dissolved in the required sterile water matched；3. 1. microbial inoculum that step is obtained is added
In 2. solution that step obtains, under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, it is sufficiently mixed in sterile blender
It is even, it is packed up to tea tree foliage bacterial fertilizer A.
Foliar fertilizer B formula compositions and preparation, (1) composition：5 parts of chitosan, 0 part of amino acid 1, fertile 15 parts micro-, 70 parts of water.
(2) it prepares：1. taking chitosan, amino acid and micro- fertilizer to be sufficiently stirred by regulation proportioning to be dissolved in the required sterile water matched, be made
Tea free leaven B.
2. field trial：
Experimental field overview：Experimental field it is located at Guizhou Zhengan County tea tree garden, kind is " Zhengan County's white tea ", and mean sea level is
1276m, subtropical zone monsoon moist climate, has a moderate climate, and 15.2 DEG C average, abundant rainfall, long annual rainfall is in 1100-1300
Between millimeter, the hydro-thermal same period.It is yellow earth for examination tea garden soil type, random in tea place before experiment, multiple spot mixing acquisition depth 0-
The soil sample of 60cm is as soil background, pH value 5.86, total nitrogen content 1.72gkg-1, the content of organic matter is
36.58g·kg-1, alkaline hydrolysis nitrogen content is 106.76mgkg-1, available phosphorus content 4.67mgkg-1, effective potassium content is
3.66mg·kg-1, effective iron content 55.36mgkg-1。
Field experimental design：RANDOMIZED BLOCK DESIGN is taken in experiment, is sprayed using complete stool (including blade face positive and negative, limb),
If 100-200 times of liquid of foliage bacterial fertilizer, 100 times of liquid of foliar fertilizer, spray clear water are control (CK), totally 3 processing, are repeated 4 times, totally 12
A cell around sets protection row.Administration time is tea tree rudiment application early period, and amount of application is 45kg/ mus.Weather is equal during experiment
Based on fine, or it is cloudy, spray in latter 3-4 days without rainfall.
3. analysis project and method：
Yield and quality：Tea polyphenols contains in investigation dry tea yield, the young sprout fresh leaf water extraction content of tealeaves, fresh tea leaves
Free aminoacid content, fresh tea leaves Determination of Chlorophyll total amount in amount, fresh tea leaves.Investigate breeding time tea tree anthracnose, moire blight,
Gall of tea incidence counts cumulative life-incidence；Incidence (%)=100 × (morbidity strain number/investigation strain number).
4. embodiment effect：
It is extremely aobvious from table 1 to table 2 as can be seen that can effectively increase tea cured leaf yield using tea tree special bio bacterial manure
It writes and improves fresh leaf water extraction, tea polyphenols, free amino acid and chlorophyll content, promote the formation of tea bush productivity and changing for quality
It is kind；And it can effectively reduce the generation of the diseases such as tea tree anthracnose, moire blight, gall of tea.
Influence of the application foliage bacterial fertilizer of table 1 to tea bush productivity quality
Note：With marking different lowercase letters after column data in P<0.05 time difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 time difference is same up to pole level of signifiance following tables
A situation arises to diseases and pests of tea tree for the application of table 2 foliage bacterial fertilizer
The specific application example of the above only present invention, the present invention also has other embodiments, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection domain of the presently claimed invention.
Claims (7)
1. a kind of tea tree foliage bacterial fertilizer, it is characterised in that：It is made from the following raw materials in parts by weight：Bacillus cercus microbial inoculum
20-40 parts, 2-8 parts of chitosan, 8-12 parts of amino acid, micro- 10-20 parts of fertilizer, 20-40 parts of water.
2. tea tree foliage bacterial fertilizer according to claim 1, it is characterised in that：It is made from the following raw materials in parts by weight：Wax
30 parts of shape gemma bacillus agent, 5 parts of chitosan, 0 part of amino acid 1, fertile 15 parts micro-, 40 parts of water.
3. tea tree foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：Contain in said waxy gemma bacillus agent
Some strains are Bacillus cercus LY05 (CCTCC NO:M 2015397), Bacillus cercus LGY06 (CCTCC NO:M
And Bacillus cercus WL08 (CCTCCNO 2015398):M2015399 a combination of one or more).
4. tea tree foliage bacterial fertilizer according to claim 3, it is characterised in that：It is prepared by said waxy gemma bacillus agent
Following steps：(1) inclined-planes bacillus cereus strain LY05, LGY06 or WL08 are preserved into test tube seed, be seeded to respectively inorganic
In salt culture medium, shaken cultivation to exponential phase obtains bacterium solution；(2) by the bacterium solution of acquisition with 10% percent by volume
Inoculum concentration is seeded in the seed fermentation tank containing minimal medium, and culture to exponential phase obtains seed liquor；(3) will
The seed liquor of acquisition is inoculated with according to the inoculum concentration of 10% volume ratio in fermentation medium, at 30-35 DEG C, 150-200rpm
Fermented and cultured 2-3 days, obtains zymotic fluid, the living bacteria count of zymotic fluid >=5,000,000,000/mL, zymotic fluid is directly diluted 5-20 times
As single bacterial strain liquid bacterial agent；(4) by the single bacterial strain liquid bacterial agent of bacillus cereus strain LY05, LGY06 or WL08
Final Bacillus cercus mix bacterium agent is mixed and made by a combination of one or more.
5. tea tree foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：The chitosan is water solubility, deacetylated
Degree is more than 95%；The amino acid be proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, lysine,
Threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate, half
Arbitrary five kinds or more of combination in cystine；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:
0.5-1:0.5-1:The mixed fertilizer of potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1；It is described
Water is sterile water.
6. a kind of preparation method of tea tree foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that：Including following technique
Step：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportioning, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportioning by regulation proportioning；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, the item of pH6.0-6.8
Under part, it is sufficiently mixed in sterile blender uniformly, it is packed up to tea tree bacterial fertilizer products.
7. a kind of application of tea tree foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that including the following aspects
Using：(1) increase the yield of tea tree；(2) improve tea tree quality；(3) induction enhancing tea tree disease resistance, effective prevention and control include tea tree
Disease including anthracnose, moire blight, gall of tea；(4) promote the growth of tea tree plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810703791.2A CN108530206A (en) | 2018-06-30 | 2018-06-30 | A kind of tea tree foliage bacterial fertilizer and the preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810703791.2A CN108530206A (en) | 2018-06-30 | 2018-06-30 | A kind of tea tree foliage bacterial fertilizer and the preparation method and application thereof |
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| CN108530206A true CN108530206A (en) | 2018-09-14 |
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ID=63487410
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810703791.2A Withdrawn CN108530206A (en) | 2018-06-30 | 2018-06-30 | A kind of tea tree foliage bacterial fertilizer and the preparation method and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108863618A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of tea tree selenium-rich liquid bacterial manure and the preparation method and application thereof |
| CN111205133A (en) * | 2020-03-11 | 2020-05-29 | 华南农业大学 | Special foliage fertilizer for golden camellia and preparation method and application thereof |
| CN112851446A (en) * | 2021-01-28 | 2021-05-28 | 于佳骏 | Foliar fertilizer for increasing large leaf yield of Pu' er tea trees and preparation method thereof |
-
2018
- 2018-06-30 CN CN201810703791.2A patent/CN108530206A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108863618A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of tea tree selenium-rich liquid bacterial manure and the preparation method and application thereof |
| CN111205133A (en) * | 2020-03-11 | 2020-05-29 | 华南农业大学 | Special foliage fertilizer for golden camellia and preparation method and application thereof |
| CN112851446A (en) * | 2021-01-28 | 2021-05-28 | 于佳骏 | Foliar fertilizer for increasing large leaf yield of Pu' er tea trees and preparation method thereof |
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Application publication date: 20180914 |