CN108911838A - A kind of stauntonvine foliage bacterial fertilizer and the preparation method and application thereof - Google Patents
A kind of stauntonvine foliage bacterial fertilizer and the preparation method and application thereof Download PDFInfo
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- CN108911838A CN108911838A CN201810703338.1A CN201810703338A CN108911838A CN 108911838 A CN108911838 A CN 108911838A CN 201810703338 A CN201810703338 A CN 201810703338A CN 108911838 A CN108911838 A CN 108911838A
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- stauntonvine
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
- Wood Science & Technology (AREA)
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- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
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- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of stauntonvine foliage bacterial fertilizers and the preparation method and application thereof, with 20-40 parts of Bacillus cercus microbial inoculum, 6-10 parts of chitosan, 6-10 parts of amino acid, micro- 10-20 parts of fertilizer, 20-40 parts of water.Applied to the yield of increase stauntonvine, improvement stauntonvine quality, improve stauntonvine storage performance, induction enhancing stauntonvine disease resistance, the growth of promotion stauntonvine plant etc..Have many advantages, such as significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease, improves yield and quality, is low in cost and easy to spread.
Description
Technical field
The present invention relates to stauntonvine cultivation techniques and fertilising field, and in particular to a kind of stauntonvine foliage bacterial fertilizer and its preparation
Method and application.
Background technique
Stauntonvine is one of third generation fruit, belongs to medical and edible dual purpose plant, because its fruits nutrition is abundant, there are many medicinal and guarantors
Health-care function and gain great popularity.Zhengan County stauntonvine amino acid element and oleanolic acid containing there are many, particularly with high super oxygen
Compound is disproportionated plum (SOD), and suppressing the hyperactive liver and easing the stomach, anticancer of drying, anti-aging, anti-inflammatory is antibacterial, reducing blood lipid, enhances immune function of human body
Great concern is at home and abroad caused etc. a variety of medicinal and healthcare function.
Zhengan County, which develops stauntonvine industry, has advantageous resources advantage, is the important breakthrough transformed agricultural production
Mouthful.In recent years, using stauntonvine industrial upgrading as breach, give full play to leading enterprise takes the lead exemplary role for Zhengan County, forms
Company+base+peasant household industrialization pattern, gradually forms the significant production, supply and sales of the relatively stable sufficient output benefit of resource, agriculture,
The industrialization management development pattern that work, trade are integrated.
But with the development of industry, administrative skill level is backward, the outstanding problems such as serious, yield and quality is low occur for pest and disease damage
It presents, and then it is extremely urgent to establish high-quality, efficient, ecological, safe stauntonvine corresponding cultivation techniques.Chemical fertilizer in agricultural production
Poor efficiency and the high environmental risk of its bring are always to perplex the significant problem in the whole world.Foliage bacterial fertilizer is by being sprayed onto leaves of plants
Can be absorbed by plant rapidly by plant leaf blade behind face, avoid from nutrient when soil application easily by soil consolidation,
The problems such as root absorption is slow has many advantages, such as to absorb fast, nutrient loss less.Currently, there are many kinds of existing foliage bacterial fertilizers,
Improving also has certain effect in the yield of crop, but all different degrees of presence raising crop yield effect is not significant etc. enough
Defect and there are no the ideal foliage bacterial fertilizers designed exclusively for stauntonvine in the prior art.
Summary of the invention
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, significant effect meet wild wood
The stauntonvine foliage bacterial fertilizer of melon growing nutrient demand.
Technical scheme is as follows：
A kind of stauntonvine foliage bacterial fertilizer, it is made from the following raw materials in parts by weight：It is prepared from the following ingredients in percentage by
Composition：20-40 parts of Bacillus cercus microbial inoculum, 6-10 parts of chitosan, 6-10 parts of amino acid, micro- 10-20 parts of fertilizer, water 20-40
Part.
Preferably, it is made from the following raw materials in parts by weight：30 parts of Bacillus cercus microbial inoculum, 8 parts of chitosan, amino
It is 8 parts sour, fertile 15 parts, 39 parts of water micro-.
Preferably, the strain contained in Bacillus cercus microbial inoculum is Bacillus cercus LY05 (CCTCC NO: M
2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more).
Wherein, Bacillus cercus microbial inoculum preparation includes the following steps：(1) by bacillus cereus strain LY05,
The inclined-plane LGY06 or WL08 saves test tube seed, is seeded in minimal medium respectively, shaken cultivation to logarithmic growth phase obtains
Obtain bacterium solution；(2) bacterium solution of acquisition is seeded to the seed containing minimal medium with the inoculum concentration of 10% percent by volume
In fermentor, culture to logarithmic growth phase obtains seed liquor；(3) by the seed liquor of acquisition according to 10% volume ratio inoculation
Amount is inoculated in fermentation medium, fermented and cultured 2-3 days at 30-35 DEG C, 150-200rpm, obtains fermentation liquid, fermentation liquid
Living bacteria count >=5,000,000,000/mL, fermentation liquid directly dilute 5-20 times as single bacterial strain liquid bacterial agent；(4) by wax-like bud
The single bacterial strain liquid bacterial agent of spore bacillus strain LY05, LGY06 or WL08 are mixed most by a combination of one or more
Whole Bacillus cercus mix bacterium agent.
Further, the chitosan is water solubility, and deacetylation is greater than 95%；The amino acid is proline, sweet ammonia
Acid, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine,
Valine, alanine, serine, arginine, asparatate, any five kinds or more of combination in cysteine；It is described
Micro- fertilizer for mass ratio is: 3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+magnesium of 0.5-1
Fertilizer+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer；The water is sterile water.
The preparation method of stauntonvine foliage bacterial fertilizer of the invention, comprises the technical steps that：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportion, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportion by regulation proportion；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH 6.0-6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to stauntonvine bacterial fertilizer products.
Bio-bacterial manure of the invention can be applied in following (1)-(4) at least one：
(1) increase the yield of stauntonvine；(2) improve stauntonvine quality, improve stauntonvine storage performance；(3) induction enhancing
Stauntonvine disease resistance, the diseases such as effective prevention and control stauntonvine leaf blight, zonate spot, root rot；(4) promote the life of stauntonvine plant
It is long；
Compared with prior art, the present invention foliage bacterial fertilizer of the present invention using Bacillus cercus microbial inoculum, plant growth-promoting substance,
The composition such as a great number of elements and microelement, can effectively improve stauntonvine economic benefit, nutrient and flavour quality and storage performance, promote
Plant well grows, and can effectively induce stauntonvine plant, the enhancing of fruit disease resistance, has significant effect, green non-pollution, fertilizer
The advantages that expecting utilization rate height, prevention and control disease, improving yield and quality, is low in cost and easy to spread.
Specific embodiment
Below with reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1：
1. bio-bacterial manure composition and preparation：
Bio-bacterial manure A formula composition and preparation, (1) composition：30 parts of Bacillus cercus microbial inoculum, 8 parts of chitosan, amino acid
8 parts, it is fertile 15 parts, 39 parts of water micro-.(2) it prepares：1. Bacillus cercus microbial inoculum is taken by regulation proportion, it is spare；2. being matched by regulation
It takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred to be dissolved in the required sterile water matched；3. 1. microbial inoculum that step is obtained is added
In 2. solution that step obtains, under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, it is sufficiently mixed in sterile blender
It is even, it is packed up to stauntonvine foliage bacterial fertilizer A.
Foliar fertilizer B formula composition and preparation, (1) composition：It is 8 parts of chitosan, 8 parts of amino acid, fertile 15 parts, 69 parts of water micro-.(2)
Preparation：1. taking chitosan, amino acid and micro- fertilizer to be sufficiently stirred by regulation proportion to be dissolved in the required sterile water matched, wild wood is made
Melon foliar fertilizer B.
2. field trial：
Experimental field it is located at the Guizhou Zhengan County town Feng Yi stauntonvine garden, kind is " Zhengan County stauntonvine " for trying orchard soil type
For yellow earth, as soil background, pH value is random in orchard before test, multiple spot mixing acquisition depth 0-60cm soil sample
5.69, total nitrogen content 1.56gkg-1, content of organic matter 30.43gkg-1, alkaline hydrolysis nitrogen content is 98.55mgkg-1,
Available phosphorus content is 14.24mgkg-1, effective potassium content is 23.41mgkg-1。
Field experimental design：RANDOMIZED BLOCK DESIGN is taken in test, using complete stool (including blade face front and back sides, limb, fruit
Deng) spraying, if 100 times of 100-200 times of liquid of foliage bacterial fertilizer, foliar fertilizer liquid, spraying clear water as control (CK), totally 3 processing, every place
5 trees are managed, are repeated 4 times, totally 12 cells, shield row is around set.Administration time is stauntonvine leaf stretching period, setting phase, nutriment
The accumulation phase applies respectively, and amount of application is respectively 30,45,60kg/ mus.Weather is based on fine during test, or cloudy, spray
It applies in latter 3-4 days without rainfall.
3. analysis project and method：
After fruit maturation, is harvested by cell, measure each cell single plant yield, 200 fruits of each cell random acquisition
It is real, take back experimental determination fruit quality, single fruit weight.Nutrient quality measuring method：Dry matter is measured with National Standard Method；Vitamin C
Content is measured with 2,6 dichlorophenol indophenol method；Titratable acid (TA) content determination of acid-basetitration；Soluble solid (SSC) contains
Amount is measured with PAL-1 type refractometer.Storage time is all rotted the time using stored peach.
Incidence and maturation history：Starting softening in fruit is every 2d investigation fruit incidence, counts accumulative morbidity
Rate；And investigate the incidences such as stauntonvine plant leaf blight, zonate spot, root rot.Disease incidence (%)=100 × (morbidity fruit
Number/investigation the total number of fruits) or disease incidence (%)=100 × (morbidity strain number/investigation strain number).Stauntonvine is observed in 7-8 month in fertilising
The growth indexes such as plant leaf SPAD value.
4. embodiment effect：
From table 1 to table 3 as can be seen that can effectively increase single plant yield, average fruit using stauntonvine foliage bacterial fertilizer
Weight significantly improves fruit dry matter, vitamin C and soluble solid (SSC) content, reduces titratable acid (TA) content, promotees
Into the formation of stauntonvine yield and the improvement of quality；Stauntonvine leaf blight, zonate spot, heart-eating worm, yellow aphids, root can also be effectively reduced
Maize ear rot etc. occurs；Effectively facilitate the growth of stauntonvine plant.
Table 1 applies influence of the foliage bacterial fertilizer to expensive long stauntonvine yield and quality
Note：It indicates with different lowercases are marked after column data in P<0.05 lower difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 lower difference is same up to extremely significant horizontal following table
Table 2 applies foliage bacterial fertilizer, and to stauntonvine disease, a situation arises
3 stauntonvine garden of table uses foliage bacterial fertilizer plant growth condition
It is above specific application example of the invention, there are other embodiments of the invention, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection scope of the presently claimed invention.
Claims (7)
1. a kind of stauntonvine foliage bacterial fertilizer, it is characterised in that：It is comprised the following components in percentage by weight：Bacillus cercus
20-40 parts of microbial inoculum, 6-10 parts of chitosan, 6-10 parts of amino acid, micro- 10-20 parts of fertilizer, 20-40 parts of water.
2. stauntonvine foliage bacterial fertilizer according to claim 1, it is characterised in that：It is made from the following raw materials in parts by weight：
It is 30 parts of Bacillus cercus microbial inoculum, 8 parts of chitosan, 8 parts of amino acid, fertile 15 parts, 39 parts of water micro-.
3. stauntonvine foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：In said waxy gemma bacillus agent
The strain contained is Bacillus cercus LY05 (CCTCC NO:M 2015397), Bacillus cercus LGY06 (CCTCC NO:M
And Bacillus cercus WL08 (CCTCCNO 2015398):M2015399 a combination of one or more).
4. stauntonvine foliage bacterial fertilizer according to claim 3, it is characterised in that：Said waxy gemma bacillus agent preparation packet
Include following steps：(1) inclined-plane bacillus cereus strain LY05, LGY06 or WL08 is saved into test tube seed, is seeded to nothing respectively
In machine salt culture medium, shaken cultivation to logarithmic growth phase obtains bacterium solution；(2) by the bacterium solution of acquisition with 10% percent by volume
Inoculum concentration be seeded in the seed fermentation tank containing minimal medium, culture obtains seed liquor to logarithmic growth phase；(3)
The seed liquor of acquisition is inoculated in fermentation medium according to the inoculum concentration of 10% volume ratio, in 30-35 DEG C, 150-200rpm
Lower fermented and cultured 2-3 days, obtain fermentation liquid, the living bacteria count of fermentation liquid >=5,000,000,000/mL, fermentation liquid is directly diluted into 5-20
It is again single bacterial strain liquid bacterial agent；(4) by the single bacterial strain liquid bacteria of bacillus cereus strain LY05, LGY06 or WL08
Agent is mixed and made into final Bacillus cercus mix bacterium agent by a combination of one or more.
5. stauntonvine foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：The chitosan is water solubility, takes off second
Acyl degree is greater than 95%；The amino acid is proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, relies ammonia
Acid, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate,
Any five kinds or more of combination in cysteine；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:
0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer of 0.5-1；It is described
Water is sterile water.
6. a kind of preparation method of stauntonvine foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that：Including following work
Skill step：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportion, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportion by regulation proportion；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, the item of pH6.0-6.8
Under part, it is sufficiently mixed in sterile blender uniformly, it is packed up to stauntonvine bacterial fertilizer products.
7. a kind of application of stauntonvine foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that including the following aspects
Application：(1) increase the yield of stauntonvine；(2) improve stauntonvine quality, improve stauntonvine storage performance；(3) induction enhancing is wild
Pawpaw disease resistance, disease of effective prevention and control including stauntonvine leaf blight, zonate spot, root rot；(4) promote stauntonvine plant
Growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810703338.1A CN108911838A (en) | 2018-06-30 | 2018-06-30 | A kind of stauntonvine foliage bacterial fertilizer and the preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810703338.1A CN108911838A (en) | 2018-06-30 | 2018-06-30 | A kind of stauntonvine foliage bacterial fertilizer and the preparation method and application thereof |
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| Publication Number | Publication Date |
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| CN108911838A true CN108911838A (en) | 2018-11-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810703338.1A Withdrawn CN108911838A (en) | 2018-06-30 | 2018-06-30 | A kind of stauntonvine foliage bacterial fertilizer and the preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112745157A (en) * | 2020-10-30 | 2021-05-04 | 广西壮族自治区农业科学院 | Selenium-enriched biological foliar fertilizer and preparation method and application thereof |
-
2018
- 2018-06-30 CN CN201810703338.1A patent/CN108911838A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112745157A (en) * | 2020-10-30 | 2021-05-04 | 广西壮族自治区农业科学院 | Selenium-enriched biological foliar fertilizer and preparation method and application thereof |
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Application publication date: 20181130 |
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