CN108522520A - A kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene - Google Patents
A kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene Download PDFInfo
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- CN108522520A CN108522520A CN201810295702.5A CN201810295702A CN108522520A CN 108522520 A CN108522520 A CN 108522520A CN 201810295702 A CN201810295702 A CN 201810295702A CN 108522520 A CN108522520 A CN 108522520A
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- thiophene
- trifloxystrobin
- gloomy copper
- bactericidal composition
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene, invention has apparent synergistic effect using trifloxystrobin and the gloomy copper of thiophene as the composite bactericide of active ingredient, delay the generation of key drug resistance, and reduce production and application costs, can be used for the improvement of resistance disease.There is preferable control effect to sigatoka.
Description
Technical field
Present invention relates particularly to technical field of pesticide combination, and in particular to a kind of sterilization group containing trifloxystrobin Yu the gloomy copper of thiophene
Close object.
Background technology
Trifloxystrobin class wide-spectrum bactericide be from natural products Strobilurins as fungicide lead compound successfully
A new class of fungicides of exploitation.With efficient, wide spectrum, protection, treats, roots out, permeates, the punching of systemic activity, resistance to rainwater
The characteristics such as brush, lasting period length.To Isosorbide-5-Nitrae-demethylation enzyme inhibitor, benzamides, dicarboxyl amine and benzimidazole generate
The bacterial strain of resistance is effective, has fungicide no interactions resistance with current.To nearly all Mycophytes (Ascomycetes, Basidiomycetes,
Oomycete and Fungi Imperfecti) disease such as powdery mildew, rust, Ying's blight, net blotch, downy mildew, rice blast etc. has good work
Property.In addition to having special efficacy to powdery mildew, leaf spot, have well to rust, downy mildew, damping-off, apple apple scab, sclerotinia sclerotiorum
Activity.To crop safety, because it is capable of being fast degraded in soil, water, thus it is environmentally safe.Due to trifloxystrobin have wide spectrum,
Infiltration, the quickly performances such as distribution, crop absorb that fast, it is with upward absorbability in addition, therefore resistance of rainwater washing against performance is good, holding effect
Phase is long, therefore is considered as second generation methoxy acrylic bactericide.Trifloxystrobin is mainly used for cauline leaf process, protection activity
It is excellent, and there is certain therapeutic activity, and activity is not affected by environment.
The gloomy copper of thiophene is thiazoles organic copper fungicide, mainly prevents bacterial blight of rice, bacterial stripe, the soft corruption of Chinese cabbage
Disease and bacterial wilt of tomato.High-efficiency broad spectrum, toxicity is low, safety and environmental protection, nuisanceless, to bacterial disease special efficacy, to fungal disease
Efficiently, stablize in acid condition, it can be with other combined use of pesticides.
It is the most effective means of controlling plant diseases using chemical agent.But it applies to long-term continuous high dose single
Chemical bactericide, be easy to cause the problems such as residual of medicament, environmental pollution and drug-resistant fungi develop.Rational chemistry
Bactericide compounded or mixture have expand fungicidal spectrum, improve control effect, extend the optimum period for applying fertilizer, reduce dosage, reduce poisoning,
The positive features such as residual, the generation for delaying drug resistance of fungi and development are reduced, bactericide compounded is to solve above-mentioned ask
One of the maximally efficient method of topic.Develop new product fungicide price is constantly soaring, and in contrast, exploitation with research efficiently,
Low toxicity, the compounding of low-residual and mixture have that small investment, the lead time is short and is paid attention to both at home and abroad, and increasing one after another develops
Dynamics.We filter out pyraclostrobin and are compounded with the gloomy copper of thiophene based on the indoor screening and field trials, have bright
Aobvious synergistic effect.And it has not been reported so far about the bactericidal composition and application of trifloxystrobin and the compounding of the gloomy copper of thiophene.
Invention content
In order to overcome the problems of the above-mentioned prior art, a kind of Bactericidal composite containing trifloxystrobin Yu the gloomy copper of thiophene is provided
Object.
The technical solution that the present invention uses is:A kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene, it is described
Bactericidal composition is compounded by trifloxystrobin and the gloomy copper of thiophene, remaining is adjunct ingredient, the quality of the trifloxystrobin and the gloomy copper of thiophene
Than being 1: 40-40: 1.
The mass ratio of the trifloxystrobin and the gloomy copper of thiophene is 2: 4.
The trifloxystrobin and the gross mass of the gloomy copper of thiophene account for the 1-30% of entire Bactericidal composite amount of substance.
The trifloxystrobin and the gross mass of the gloomy copper of thiophene account for the 30% of entire Bactericidal composite amount of substance.
The bactericidal composition be missible oil, suspending agent, wettable powder, water dispersible granules, aqueous emulsion, microemulsion,
One kind in granula, microcapsule formulations.
The beneficial effects of the invention are as follows:The present invention provides a kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene, hair
It is bright that there is apparent synergistic effect as the composite bactericide of active ingredient using trifloxystrobin and the gloomy copper of thiophene, delay the production of key drug resistance
It is raw, and production and application costs are reduced, it can be used for the improvement of resistance disease.There is preferable prevention to sigatoka
Effect.
Specific implementation mode
With reference to embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention,
It is not limitation of the invention.
Embodiment:Trifloxystrobin and the gloomy copper compounding prevention sigatoka formula indoor screening test of thiophene
Subjects:Sigatoka bacterium
Reagent agent:
Pharmacy type | Chinese/dosage form/content | Unit is provided |
Test medicine | 97% trifloxystrobin TC | The Nantong chemical inc Tai He |
Test medicine | The gloomy copper TC of 92% thiophene | Zhejiang east wind Chemical Co., Ltd. |
Test method and technical essential:
Test method:Plating.Evaluation method:The bioactivity of fungicide is evaluated with Toxicity baseline and EC50, it is comprehensive
EC50 and co-toxicity coefficient evaluate drug combination type of action.
Test result:
Conclusion:
Trifloxystrobin and the gloomy copper of thiophene have good bacteriostatic activity to sigatoka bacterium.There is apparent increase after the two compounding
Effect acts on.In conjunction with indexs such as EC50 and co-toxicity coefficients, the proportioning 2: 4 of trifloxystrobin and the gloomy copper of thiophene that enterprise provides has reasonability,
It can further research and develop.
Test objective
By Zhejiang, east wind Chemical Co., Ltd. is entrusted, and using Plating, tests trifloxystrobin and the gloomy copper single dose of thiophene to banana
The indoor bactericidal activity of leaf spot fungi, and 5 proportionings are devised, verification screening is carried out, is carried for field trial and next step research and development
For foundation.
Experimental condition
For trying pathogen
Sigatoka bacterium, it is indoor to preserve throughout the year.
Instrument and equipment
Sartorius BP211D 0.01mg electronic balances, eppendorf research 100-1000 μ l, liquid-transfering gun,
Microscope, pipette, conical flask, graduated cylinder, beaker, card punch, slide calliper rule, culture dish, stainless steel medicine spoon, electric heating forced air drying
Case, incubator etc..
Experimental design
Reagent agent
97% trifloxystrobin TC (offer of the Nantong chemical inc Tai He);
The gloomy copper TC of 92% thiophene (offer of Zhejiang east wind Chemical Co., Ltd.).
Test dose designs
It is matched according to trifloxystrobin and the compounding of the gloomy copper of thiophene 2: 4, sets 1: 5,1.5: 4.5,2: 4,2.5: 3.5,3: 3 etc. 5 altogether
Combination, specific test dose design are shown in Table 1.
Processing mode
Processing time
On January 24, -2018 years on the 21st January in 2018
Administrated method
Plating
Chemicals treatment
The gloomy each 1000mg of copper TC of thiophene of 97% trifloxystrobin TC and 92% are weighed with electronic balance, and N, N- dimethyl methyls is added
Amide dissolves, spare with 20000mg/L mother liquors are diluted to containing 0.2% Tween 80 aqueous solution.
The preparation of toxic medium
Table is designed according to 1 test dose of table, each test medicament and the PSA culture mediums prepared mutually are blended into drug concentration
For 2000mg/L, 1000mg/L, 500mg/L, 250mg/L, 125mg/L, 62,5mg/L, 31.25mg/L, 15.625mg/L,
The toxic tablet of 7.813mg/L and 3.906mg/L is for use, and each processing sets 4 repetitions, while setting solvent-laden water and milk solution and making
Blank control.
Inoculation method
By cultured pathogen, the sterilization punchers of diameter 5mm are aseptically used, bacterium is cut from colony edge
Cake, with inoculator by pure culture biscuits involvng inoculation in drug containing tablet center, mycelia is face-up, covers ware lid, sets in 25 DEG C of incubators and cultivates.
Investigation method:
Investigation method:It is measured with slide calliper rule after cultivating 3 days in the incubator and measures colony growth diameter (mm), each bacterium colony is used
Crossing method vertical measurement diameter is each primary, takes its average value, and the diameter that bacterium colony is grown is calculated after deducting bacteria cake diameter.
Data statistic analysis:
Computational methods
According to investigation result, each concentration for the treatment of is calculated to the mycelial growth inhibition rate for trying target bacterium by formula (1), (2),
Unit is percentage (%), and result of calculation retains 2 significant digits.
D=D1-D2............. (1)
In formula:D- bacterium colonies increase diameter;Mono- colony diameters of D1;Mono- bacteria cake diameters of D2.
I=(D0-Dt) × 100/D0............. (2)
In formula:Mono- mycelial growth inhibition rates of I;Mono- blank control mycelia growth diameters of D0;Mono- chemicals treatment bacterium colonies of Dt increase
Diameter.
Statistical disposition
Make regression analysis according to each drug concentration logarithm and corresponding mycelial growth inhibition rate probit value, each medicament
EC50,95% confidence limit.The total poison system (CTC) of mixture is calculated according still further to Sun Yunpei's Method.The compounding agents of CTC >=120 show synergy;
The compounding agents of CTC≤80 show antagonism:80 < CTC <, 20 compounding agents show as being added.Specific formula for calculation is as follows:
ATI=S × 100/M.............. (3)
The toxicity index that mono- mixtures of ATI are surveyed in formula;The EC50 of mono- standard agents of S, unit are milligrams per liter (mg/L);M
One for try mixture EC50, unit be milligrams per liter (mg/L).
TTI=TIA × PA+TIB × PB....... (4)
The theoretical toxicity index of TTI- mixtures in formula;TIA-A medicament toxicity index;Percentage of the PA-A medicaments in intermixture
Content, unit are percentage (%);TIB-B medicament toxicity index;Percentage composition of the PB-B medicaments in intermixture, unit hundred
Divide rate (%).
CTC=ATI × 100/TTI....... (5)
CTC- co-toxicity coefficients in formula;ATI- mixture surveys toxicity indexes;TTI- mixtures theoretical toxicity indexes.
Toxicity test
2 trifloxystrobin of table and the gloomy copper single dose of thiophene and 5 compoundings are with comparison sigatoka plate measurement result
5 EC50 values with comparison sigatoka of trifloxystrobin and the gloomy copper compounding of thiophene are 13.9596 as can be seen from Table 2
~15.7165mg/L, 2 medicaments are respectively less than 2 single doses by EC50 after this experimental design proportioning compounding.
Synergy is evaluated
5 compoundings of 3 trifloxystrobin of table and the gloomy copper of thiophene are with the synergy evaluation for comparing sigatoka
5 co-toxicity coefficients with comparison sigatoka of trifloxystrobin and the gloomy copper compounding of thiophene are as can be seen from Table 3
121.72~134.57, all have synergistic effect.
Conclusion
Trifloxystrobin and the gloomy copper of thiophene have good bacteriostatic activity to sigatoka bacterium.There is apparent increase after the two compounding
Effect acts on.In conjunction with indexs such as EC6 and co-toxicity coefficients, the proportioning 2: 4 of trifloxystrobin and the gloomy copper of thiophene that enterprise provides has reasonability, can
Further to research and develop.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (5)
1. a kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene, which is characterized in that the bactericidal composition is by trifloxystrobin
It is compounded with the gloomy copper of thiophene, remaining is adjunct ingredient, and the mass ratio of the trifloxystrobin and the gloomy copper of thiophene is 1: 40-40: 1.
2. a kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene according to claim 1, which is characterized in that described
The mass ratio of trifloxystrobin and the gloomy copper of thiophene is 2: 4.
3. a kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene according to claim 1, which is characterized in that described
Trifloxystrobin and the gross mass of the gloomy copper of thiophene account for the 1-30% of entire Bactericidal composite amount of substance.
4. a kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene according to claim 3, which is characterized in that described
Trifloxystrobin and the gross mass of the gloomy copper of thiophene account for the 30% of entire Bactericidal composite amount of substance.
5. a kind of bactericidal composition containing trifloxystrobin Yu the gloomy copper of thiophene according to claim 1, which is characterized in that described
Bactericidal composition is in missible oil, suspending agent, wettable powder, water dispersible granules, aqueous emulsion, microemulsion, granule, microcapsule formulations
One kind.
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Citations (1)
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CN104222116A (en) * | 2014-09-16 | 2014-12-24 | 浙江东风化工有限公司 | Saisentong-containing agricultural bactericidal composition |
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CN104222116A (en) * | 2014-09-16 | 2014-12-24 | 浙江东风化工有限公司 | Saisentong-containing agricultural bactericidal composition |
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