CN106982844B - A kind of agricultural bactericidal composition of Bao Han famoxadones and hymexazol - Google Patents
A kind of agricultural bactericidal composition of Bao Han famoxadones and hymexazol Download PDFInfo
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- CN106982844B CN106982844B CN201710336226.2A CN201710336226A CN106982844B CN 106982844 B CN106982844 B CN 106982844B CN 201710336226 A CN201710336226 A CN 201710336226A CN 106982844 B CN106982844 B CN 106982844B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/76—1,3-Oxazoles; Hydrogenated 1,3-oxazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
- A01N47/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom containing >N—S—C≡(Hal)3 groups
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Abstract
The present invention relates to a kind of bactericidal composition of Han famoxadones and hymexazol, it is 1 that the bactericidal composition, which contains weight ratio,:20‑20:1 famoxadones and hymexazol.Further, can also include third component selected from the group below in the bactericidal composition：Triazolone, Triadimenol, metrafenone, Fluoxastrobin, captan or folpet；The weight ratio of the Famoxate, hymexazol and third component is：1‑10:1‑10:1:1‑3.Further, the present invention also provides the sterilization sustained release agents using bactericidal composition of the present invention made of specific composite slow release agent.Compared with single dose, binary or ternary bactericidal composition of the present invention all has significant synergistic effect, and coefficient of synergism is 130 or more；The present invention sterilizes sustained release agent using specific sustained release agent is obtained, has lasting slow releasing function, and have good stability.
Description
Technical field
The invention belongs to technical field of compounded pesticide, are related to a kind of bactericidal composition, and in particular to one kind includes Yi oxazoles
Bacterium ketone, the agricultural bactericidal composition that hymexazol is fungicide active ingredient.The invention further relates to the bactericidal compositions to have sustained release
The preparation formulation of effect and its in application agriculturally.
Famoxadone, English name：Famoxadone, chemical name：3- anilino- -5- methyl -5- (4- phenoxy group benzene
Base) -1,3- oxazoline -2,4- diketone , Shu oxazole series bactericidal agents.Famoxadone belongs to Mitochondrial electron and transmits inhibitor, to compound
Cytochrome c oxidoreductase has an inhibiting effect in body m, also have protect, treat, rooting out, permeating, systemic activity.Oxazole bacterium
Ketone is as a kind of new and effective, wide-spectrum bactericide, suitable for crop such as wheat, barley, pea, beet, rape, grape, Ma Ling
Potato, pawl class, capsicum, tomato etc. are mainly used for preventing the important disease such as white powder in Ascomycetes, Basidiomycetes, oomycetes subclass
Disease, rust, glume blight, net blotch, downy mildew, late blight etc..
Hymexazol, English name are：Hymexazol, chemical name are：3-hydroxy-5-methylisoxazole belongs to oxazole class and kills
Microbial inoculum.The medicament belongs to efficient, less toxic, environmentally friendly, broad-spectrum germicide, has preferably to plant disease caused by a variety of disease fungus
Control effect, to mastigomycetes, sac fungus, basidiomycetes, the pythium spp of Deuteromycotina, sickle-like bacteria, rhizoctonia, photovoltaicing leather bacteria, snow
Rotten bacterium etc. has good therapeutic effect.It is to inhibit pathogen line grain that hymexazol, which has interior suction and conduction, bactericidal mechanism,
Body respiration, but it is different from beta-methoxy acrylic ester fungicide.Meanwhile hymexazol is a kind of soil sterilants again, it is right
Pythium spp in soil has efficiently, can improve the sprouting ability for inhibiting germ spore with inorganic aluminum ions binding in the soil.It dislikes
Mould spirit is also act as plant growth regulator, and the metabolite in plant is two kinds of glucosides, can improve plant life
Activity is managed so as to promote the activity of the tiller of plant strain growth, root, the increase of root hair and root to improve.Hymexazol is to micro- life in soil
The ecology of object does not have an impact, and can resolve into the very low compound of toxicity, safe noresidue in the soil.
Hymexazol is the larger fungicide kind of current domestic dosage, but is found in use, the biodegradation of hymexazol
Excessive velocities just lose bactericidal effect in only 5-10 days under conditions of use.And the duration that germ breaks out every time is generally 15
It is to a wheat harvesting period, so the method for dispenser repeatedly is usually taken in hymexazol when in use, i.e., it is primary per dispenser in 5-10 days, until
Germ is terminated stage of attack.Obviously, from artificial angle is saved, the method for dispenser is very uneconomical repeatedly, time-consuming and laborious, and peasant is urgent
Need a kind of preparation containing hymexazol of slow-release.
In the field of agrochemicals containing hymexazol active component, patent document have been disclosed hymexazol respectively with double alkynes acyls
Bacterium amine（CN105325428A）, mepanipyrim（CN105379731A）, fludioxonil（CN105104386A）, 2-cyano-3-amino-3-phenylancryic acetate
（CN104094942A）The bactericidal composition of compounding, but these bactericidal composition effects are general, and do not pay close attention to sustained release work(
The preparation formulation of effect.Patent CN103947673A discloses a kind of antibacterial root-growing agent of sustained release, for by sustained release agent, fungicide, reinforcing
The paste colloid that agent, root-growing agent and lytic agent are mixed to get, sterilization component can select hymexazol.The paste that the patent is related to
Colloid belongs to physical sustained release agent.The sustained-release pesticides containing ammonium hydrogen carbonate are discussed in patent US5432148, it is mentioned that hymexazol
Ingredient belongs to physical sustained release agent.Patent CN1419826A gives the polymer chemical slow release sterilization of the ingredient containing hymexazol
Hymexazol is linked on macromolecule carrier, extends the drug effect lasting period of hymexazol by agent；However these sustained-release dosage types are in the presence of slow
Release the deficiencies of ineffective, bad with the compatibility of active constituent.
Invention content
Having that bactericidal effect is good, the low stimulation of low toxicity the object of the present invention is to provide one kind, synergistic effect is notable after compounding, and
And drug resistance famoxadones and the bactericidal composition of hymexazol can be delayed.
To achieve the purpose of the present invention, technical scheme is as follows：
A kind of bactericidal composition of Han famoxadones and hymexazol, which is characterized in that the bactericidal composition contains weight
Amount is than being 1:20-20:1 famoxadones and hymexazol.
It is preferred that the weight ratio of the two is 1:5-5:1.
Further, can also include third component selected from the group below in the bactericidal composition：Triazolone, Triadimenol,
Metrafenone, Fluoxastrobin, captan or folpet；The weight ratio of the Famoxate, hymexazol and third component is：1-10:1-
10:1:1-3；It is highly preferred that the weight ratio of three is 1-5:1-5:1-2, most preferably 3:3:1.
Further, the present invention provides the sterilization sustained release agent of above-mentioned bactericidal composition, which is characterized in that the sustained release agent by
Following group by weight percentage is grouped as：
Bactericidal composition：1-15%；
Sustained release agent：50-70%；
Hardening agent：10-30%；
Surplus is water；
The hardening agent is aluminum sulfate, copper sulphate or ferrous sulfate；
The sustained release agent is that weight ratio is 1:2-4:The composite slow release agent of 1 sodium bentonite and cyclodextrin composition.
Sterilization sustained release agent preparation method of the present invention is as follows：
Step 1: according to described with each raw material of measurement；
Step 2: active constituent and hardening agent are added in water successively, stir evenly；
Step 3: the composite slow release agent of sodium bentonite and cyclodextrin composition is added and stirs, mixture is made fully to merge
At paste colloid.
Preferably, in the sterilization sustained release agent, bactericidal composition 5-10%；Most preferably 8%.
Preferably, the sustained release agent is 60%, and the sustained release agent is that weight ratio is 1:1-3:1 sodium bentonite and ring paste
The composite slow release agent of essence composition；More preferably weight ratio is 2:The composite slow release agent of 1 sodium bentonite and cyclodextrin composition.
Unless otherwise indicated, " % " of the invention is weight percentage.
Further, the present invention is provided above-mentioned bactericidal composition or sterilization sustained release agent for preventing cucumber downy mildew, horse
Bell potato late blight, the purposes of watermelon blight or capsicum damping-off.
Principle is explained：The sterilization sustained release agent of the present invention focuses on be especially suitable for seedbed sustained release agent to wherein closing
The selection of key component sustained release agent：First, sodium bentonite itself is the nonmetallic minerals using montmorillonite as main mineral constituent,
There are certain cations for the layer structure that montmorillonite structure cell is formed, when sodium bentonite is contacted with water, these interlayer cations
Have the tendency that it is a kind of into water dissociate, spread, with cation disengaging, bentonite surface is negatively charged, at this time can be in solution
Cation generate Electrostatic Absorption, a portion cation and sodium bentonite are closely attracting, form adsorption layer, remaining is positive
Ion is evenly distributed in the liquid phase of surrounding with their aqueous solvent and forms diffusion layer；It is pasted when by sodium bentonite and ring
When essence is combined with special ratios, the two compatibility is good, can preferably excite the electric double layer characterization of adsorption of sodium bentonite so that kill
Bacterium active constituent, at condensation product, has very strong stability and lasting slow release by adsorpting aggregation.
Compared with prior art, beneficial effects of the present invention are：
（1）Compared with single dose, binary or ternary bactericidal composition of the present invention all has significant synergistic effect, increases
Coefficient is imitated 130 or more；
（2）The present invention sterilizes sustained release agent using specific sustained release agent is obtained, has lasting slow releasing function, and stability is good
It is good.
Specific implementation mode
Purpose, technical solution and its effect to facilitate the understanding of the present invention, now will in conjunction with the embodiments to the present invention do into
One step elaborates.
One, biological Examples
Embodiment 1, cucumber downy mildew indoor bioassay
In order to prevent the downy mildew in agricultural production, inventor Yi famoxadones, hymexazol have carried out the increasing of mutual compounding
Effect research, specific method are：
Experiment uses bacterium of downy mildew of cucumber（Pseudoperonospora cubensis）For test object.Active compound is prepared
At the test medicine of needs, test method reference《People's Republic of China's agricultural industry criteria NY/T1156.3-2006》.
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy first（It is antibacterial on the basis of pilot study result
Rate is set in the range of 5%~90% by geometric progression）.Experiment target bacterium is the Huang for picking up from the field leaf position of 4 leaves~6 from up to down
Melon Pseudoperonospora cubensis blade washes the Pseudoperonospora cubensis sporangium at the inferior lobe piece back side with 4 DEG C of distillations, is made into suspension（Concentration control exists
Every milliliter 1 × 105A~1 × 107A sporangium）, store at 4 DEG C spare.Liquid is uniformly sprayed on to the healthy leaves back side, is waited for
After liquid natural air drying, by each processing blade and blade backwards to upper, it is emitted in moisture preservation box after being marked by processing.Experiment is set without medicament
Processing make blank control.It is inoculated in vacuum side of blade with ready 10 μ L of Fresh spores capsule suspension drop.Per blade inoculation 4
Drop, often processing are no less than 5 leaves.Culture ware lid is covered after inoculation, be placed in growth cabinet or has the humidistat of illumination, daily
Continuous illumination/dark 12h replaces, 17 DEG C~22 DEG C of temperature, is cultivated under conditions of 90% or more relative humidity.Depending on processing and blank
Control incidence measures and records lesion diameter, and unit is millimeter（mm）, calculate control effect.Pass through the probability of control effect
Linear regression analysis between value and the logarithm of series concentration, finds out the EC of each medicament50 Value calculates mixture with Sun Yunpei's Method
Co-toxicity coefficient（CTC）, activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient of complex preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜
CTC ＜ 120 show as summation action.
Cucumber downy mildew The Toxicity Determination result described in the present embodiment is shown in Table 1.
1 famoxadone of table measures the synergy of cucumber downy mildew with hymexazol mixture
, famoxadones and hymexazol are respectively with 1 as can be seen from Table 1:20、1:15、1:10、1:8、1:5、1:2、1:1、2:
1、5:1、8:1、10:1、15:1、20:1 combination to cucumber downy mildew CTC values be 167.61,171.42,163.93,172.51,
163.84,143.29,155.95,171.69,148.72,144.98,133.10,174.57,146.54, Shuo Ming famoxadones with
Hymexazol is with mass ratio 1:20~20:1 is applied in combination and has synergistic effect to cucumber downy mildew germ, can be used for preventing cucumber cream
Mildew.
Embodiment 2, late blight of potato indoor bioassay
Subjects are the late blight of potato（Potato Late Bright）.The investigational agent that active compound is configured to need
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy by agent（On the basis of pilot study result, bacteriostasis rate 5%~
It is set by geometric progression in the range of 90%）.If clear water compares, it is repeated 3 times.Experiment uses mycelial growth rate method, will cultivate
Pathogen cut bacteria cake from colony edge aseptically with a diameter of 5 mm card punch, bacteria cake is connected to transfer needle
On the culture medium flat plate of different agents concentration, 28 DEG C are set, cultivate in saturated humidity incubator；When control group bacterium colony covers with, measure
Colony diameter calculates the percentage that each chemicals treatment inhibits mycelia growth, passes through the probit value of inhibiting rate and pair of series concentration
Linear regression analysis between numerical value finds out the EC of each medicament50Value calculates the co-toxicity coefficient of mixture with Sun Yunpei's Method（CTC）,
Activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient of complex preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜
CTC ＜ 120 show as summation action.
Late blight of potato The Toxicity Determination result described in the present embodiment is shown in Table 2.
2 famoxadone of table measures the synergy of the late blight of potato with hymexazol mixture
, famoxadones and hymexazol are respectively with 1 as can be seen from Table 2:20、1:15、1:10、1:8、1:5、1:2、1:1、2:
1、5:1、8:1、10:1、15:1、20:1 combination to late blight of potato CTC values be 148.77,150.69,158.51,
168.73,171.04,177.54,156.80,171.66,160.20,147.04,142.01,129.29,139.89, Shuo Ming Evil
Cycloheximide triazole is with hymexazol with mass ratio 1:20~20:1 is applied in combination and has synergistic effect to late blight of potato germ, can be used for
Prevent the late blight of potato.
Embodiment 3, watermelon blight indoor bioassay
Subjects are watermelon point fusarium（Fusarium oxysporum）.By famoxadone, hymexazol active compound, according to
The matched proportion density of setting is configured to required liquid, spare.Experiment uses mycelial growth rate method.
Aseptically, the culture medium melted in advance is quantitatively adding in sterile conical flask, from low concentration to high concentration
Liquid is quantitatively drawn successively, is separately added into above-mentioned conical flask, is fully shaken up.Then equivalent pours into the culture dish of 4 a diameter of 9cm
In, the drug containing tablet of respective concentration is made.Processing without medicament is repeated 4 times as blank control.
By cultured pathogen, aseptically, with the card punch of diameter 5mm, bacteria cake is cut from colony edge, used
Bacterium disease is inoculated in drug containing tablet center by inoculator, and mycelia is face-up, covers ware lid, sets 26 DEG C of incubator culture.According to sky
The growing state of bacterium investigates pathogen mycelia growing state in white control culture dish, with vernier caliper measurement colony diameter, cross
Interior extrapolation method is vertically respectively surveyed once, is averaged.
Synergistic effect of the bactericidal composition provided by the invention to watermelon blight is evaluated using co-toxicity coefficient method, with medicament
The logarithm and preventive effect niqueMin of concentration seek regression equation, and the EC of medicament pair is calculated50Value, then acquires co-toxicity coefficient.With
Co-toxicity coefficient evaluates synergy of the compound drug to watermelon blight, co-toxicity coefficient>120 are expressed as synergistic effect, altogether poison system
Number is expressed as summation action, co-toxicity coefficient between 80~120<80 are expressed as antagonism.
3 famoxadone of table measures the synergy of watermelon blight with hymexazol mixture
, famoxadones and hymexazol are respectively with 1 as can be seen from Table 3:20、1:15、1:10、1:8、1:5、1:2、1:1、2:
1、5:1、8:1、10:1、15:1、20:1 combination to watermelon point fusarium CTC values be 148.87,159.21,163.47,160.13,
154.02,143.64,174.52,149.24,141.44,132.80,133.30,145.43,125.75, Shuo Ming famoxadones with
Hymexazol is with mass ratio 1:20~20:1 is applied in combination and has synergistic effect to watermelon blight, can be used for preventing potato late blight
Disease.
Embodiment 4, capsicum damping-off indoor bioassay
Subjects are Rhizoctonia solani Kuhn（Rizoctonia solani）.By famoxadone, hymexazol active compound, according to setting
Fixed matched proportion density is configured to required liquid, spare.Experiment uses mycelial growth rate method.
Aseptically, the culture medium melted in advance is quantitatively adding in sterile conical flask, from low concentration to high concentration
Liquid is quantitatively drawn successively, is separately added into above-mentioned conical flask, is fully shaken up.Then equivalent pours into the culture dish of 4 a diameter of 9cm
In, the drug containing tablet of respective concentration is made.Processing without medicament is repeated 4 times as blank control.
By cultured pathogen, aseptically, with the card punch of diameter 5mm, bacteria cake is cut from colony edge, used
Bacterium disease is inoculated in drug containing tablet center by inoculator, and mycelia is face-up, covers ware lid, sets 25 DEG C of incubator culture.According to sky
The growing state of bacterium investigates pathogen mycelia growing state in white control culture dish, with vernier caliper measurement colony diameter, cross
Interior extrapolation method is vertically respectively surveyed once, is averaged.
Synergistic effect of the bactericidal composition provided by the invention to capsicum damping-off is evaluated using co-toxicity coefficient method, with medicament
The logarithm and preventive effect niqueMin of concentration seek regression equation, and the EC50 values of medicament pair are calculated, and then acquire co-toxicity coefficient.With
Co-toxicity coefficient evaluates synergy of the compound drug to capsicum damping-off, co-toxicity coefficient>120 are expressed as synergistic effect, altogether poison system
Number is expressed as summation action, co-toxicity coefficient between 80~120<80 are expressed as antagonism.
4 famoxadone of table measures the synergy of capsicum damping-off with hymexazol mixture
, famoxadones and hymexazol are respectively with 1 as can be seen from Table 4:20、1:15、1:10、1:8、1:5、1:2、1:1、2:
1、5:1、8:1、10:1、15:1、20:1 combination Rhizoctonia solani CTC values be 146.70,141.48,155.36,159.07,
161.46,174.67,151.41,167.97,163.14,159.27,147.18,140.66,130.31, Shuo Ming famoxadones with
Hymexazol is with mass ratio 1:20~20:1 is applied in combination and has synergistic effect to capsicum damping-off, can be used for preventing capsicum stand it is withered
Disease.
In addition, the ternary bactericidal composition of the present invention, compares single component, and compared to the component of binary, it is same to have
There are synergistic effect, coefficient of synergism to be also all higher than 120；Such as the ternary formed Yi famoxadone, hymexazol and Fluoxastrobin is killed
For bacteria composition, Xiang Bi famoxadones, three specific monomers of hymexazol or Fluoxastrobin and Xiang Bi famoxadones and evil are mould
Spirit, hymexazol and Fluoxastrobin, three binary compositions formed Yi Ji famoxadones and Fluoxastrobin, all have synergistic effect, increase
It imitates coefficient and is more than 120.
Two, embodiment is prepared
Prepare embodiment 1：4 Chong Liang Fen famoxadones, 4 parts by weight hymexazols and 15 parts sulfuric acid aluminium are added into water
In, it stirs evenly, 20 parts by weight sodium bentonites and 40 parts by weight of cyclodextrin is then added, stir the mixture for uniformly, making to mix
It closes object and is fully fused into paste colloid, finished product.
Prepare embodiment 2：In addition to 48 parts by weight sodium bentonites and 12 parts by weight of cyclodextrin are added, remaining is implemented with preparation
Example 1；
Prepare embodiment 3：In addition to 30 parts by weight sodium bentonites and 30 parts by weight of cyclodextrin are added, remaining is implemented with preparation
Example 1；
Prepare embodiment 4：In addition to 45 parts by weight sodium bentonites and 15 parts by weight of cyclodextrin are added, remaining is implemented with preparation
Example 1；
Prepare embodiment 5：In addition to 40 parts by weight sodium bentonites and 20 parts by weight of cyclodextrin are added, remaining is implemented with preparation
Example 1；
Comparative example 1：In addition to 60 parts by weight sodium bentonites are added, remaining is the same as preparation embodiment 1；
Comparative example 2：In addition to 60 parts by weight of cyclodextrin are added, remaining is the same as preparation embodiment 1；
Comparative example 3：In addition to 60 parts by weight talcum powder are added, remaining is the same as preparation embodiment 1；
Comparative example 4：In addition to 15 parts by weight sodium bentonites and 45 parts by weight of cyclodextrin are added, remaining is implemented with preparation
Example 1；
Comparative example 5：In addition to 50 parts by weight sodium bentonites and 10 parts by weight of cyclodextrin are added, remaining is implemented with preparation
Example 1.
Three, field control effectiveness test.
1, cucumber downy mildew field control effectiveness test
Carried out prevention cucumber downy mildew field trial in Shouguang City of Shandong Province within 2016, demonstrate above-mentioned test medicine and
Control effect of the contrast agents to cucumber downy mildew and the safety to cucumber.
Trial crops are cucumber, and controlling object is cucumber downy mildew（Pseudoperonospora cubensis）.Experiment
It is located at Shouguang City rice field rice fields Zhen Dong village, experimental plot topography is flat, and soil is loam, middle fertility, pH value 6.9, during experiment
Fertilizer and water management is medium.Test medicine and dosage refer to table 5.Blank control separately is set, often handles 4 repetitions, per cell 30 ㎡, totally 10
A cell, random district's groups arrangement.Using conventional spray-on process, mu dispenser liquid 45kg is uniformly sprayed at leave dual sides.
Investigation and statistical method：State of an illness radix is investigated before dispenser, 10~14d is reinvestigated once after last time dispenser, altogether
Investigation 2 times.Take at random per cell at 4 points, investigate 2 plants, and the whole blades of every plant of investigation, every leaf accounts for leaf area according to scab at every
Percentage classification record.Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for 5% or less entire leaf area；
3 grades：Lesion area accounts for the 6%~10% of entire leaf area；
5 grades：Lesion area accounts for the 11%~25% of entire leaf area；
7 grades：Lesion area accounts for the 26%~50% of entire leaf area；
9 grades：Lesion area accounts for 50% or more of entire leaf area.
Disease index=[∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total leaf number × 9)] × 100
Control effect (%)=[1- (disease index after disease index × processing dispenser before control dispenser)/（Disease after control dispenser
Disease index before feelings index × processing dispenser）×100
Table 5 prevents cucumber downy mildew field test results
As can be seen from the above table, example of formulations of the invention is better than comparative example, especially when example of formulations 5 is selected
When selecting the composite slow release agent of special ratios, best results；Compared to single sustained release agent, other sustained release agents and in ratio of the present invention
Composite slow release agent outside range, the present invention have more preferably control effect, show that its slow release effect is more excellent.
Security survey to cucumber is observed after spray for several days after the 1st day and medicine, and each test process of the present invention is to cucumber
No poisoning phenomenon occurs.
2, late blight of potato field control effectiveness test
The 7-8 months in 2016 have carried out prevention late blight of potato field trial in Fugu County of Shaanxi Province.Test arrangement is being gone through
The plot of year morbidity weight, test medicine and dosage are shown in Table 6, separately set blank control, 4 repetitions are often handled, per plot area 25
㎡, totally 10 cells, random district's groups arrange.Using conventional spray-on process, blade positive and negative uniformly squirt, and 50 liters of liquid dosage/
Mu.When seeing the blade beginning, second of dispenser after 14d, dispenser is secondary altogether for first time dispenser.
Investigation and statistical method：14 d carry out disease index investigation after second of dispenser, are taken for 5 points per cell diagonal line
Sample, every is investigated 3 plants, investigates whole blades, and record total number of sheets, the sick number of sheets at different levels and sick series calculate disease index.
Grade scale is:
0 grade, disease-free spot；
1 grade, lesion area accounts for 5% or less entire leaf area；
3 grades, lesion area accounts for the 6%-10% of entire leaf area；
5 grades, lesion area accounts for the 11%-20% of entire leaf area；
7 grades, lesion area accounts for the 21%-50% of entire leaf area；
9 grades, lesion area accounts for 50% or more of entire leaf area.
Disease index and control effect computational methods：
Disease index=Σ（The disease numbers of sheets at different levels × opposite value of series）/（Investigate total number of sheets × 9）×100
Control effect（%）=[（Disease index after disease index-chemicals treatment district dispenser after blank district dispenser）/ empty
Disease index after white check plot dispenser] × 100
Table 6 prevents late blight of potato field test results
As can be seen from the above table, example of formulations of the invention is better than comparative example, especially when example of formulations 5 is selected
When selecting the composite slow release agent of special ratios, best results；Compared to single sustained release agent, other sustained release agents and in ratio of the present invention
Composite slow release agent outside range, the present invention have more preferably control effect, show that its slow release effect is more excellent.
Security survey to potato is observed, at each medicament of the present invention for several days after the 1st day and medicine after spray every time
Potato occurs without poisoning phenomenon for reason.
3, watermelon blight field control effectiveness test
In August, 2016 has carried out prevention watermelon blight field trial in Shaanxi Province Weinan City Dali County.Test arrangement exists
The plot of morbidity weight over the years, test medicine and dosage are shown in Table 7, separately set blank control, 4 repetitions are often handled, per plot area
30 ㎡, totally 10 cells, random district's groups arrange.Using conventional spray-on process, 45 liter/mu of liquid dosage.Disease initial stage applies for the first time
Medicine, second of dispenser after 14d, dispenser is secondary altogether.
Investigation and statistical method：Whether all plant have the classical symptom of droop in investigation cell, record droop
Strain number of falling ill and investigation total strain number.
Disease index and control effect computational methods：
Diseased plant rate（%）=diseased plant number/investigation total strain number × 100
Control effect（%）=（Blank district diseased plant rate-chemicals treatment district diseased plant rate）/ blank district diseased plant rate ×
100
Table 7 prevents watermelon blight field test results
As can be seen from the above table, example of formulations of the invention is better than comparative example, especially when example of formulations 5 is selected
When selecting the composite slow release agent of special ratios, best results；Compared to single sustained release agent, other sustained release agents and in ratio of the present invention
Composite slow release agent outside range, the present invention have more preferably control effect, show that its slow release effect is more excellent.
Security survey to watermelon is observed, each chemicals treatment of the present invention for several days after the 1st day and medicine after spray every time
Watermelon is occurred without poisoning phenomenon.
4, capsicum damping-off field control effectiveness test
In October, 2016 has carried out prevention capsicum damping-off field trial in Baoji, Shaanxi province city Mei County.Test arrangement is being gone through
The plot of year morbidity weight, test medicine and dosage are shown in Table 8, separately set blank control, 4 repetitions are often handled, per plot area 20
㎡, totally 10 cells, random district's groups arrange.Using conventional spray-on process, blade positive and negative uniformly squirt, and 45 liters of liquid dosage/
Mu.Disease first time at initial stage dispenser, second of dispenser after 14d, dispenser is secondary altogether.
Investigation and statistical method：Per the 5 points of samplings of cell diagonal line, every is investigated 20 plants, records morbidity strain number and total strain number.
Disease index and control effect computational methods：
Diseased plant rate（%）=diseased plant number/investigation total strain number × 100
Control effect（%）=（Blank district diseased plant rate-chemicals treatment district diseased plant rate）/ blank district diseased plant rate ×
100
Table 8 prevents capsicum damping-off field test results
As can be seen from the above table, example of formulations of the invention is better than comparative example, especially when example of formulations 5 is selected
When selecting the composite slow release agent of special ratios, best results；Compared to single sustained release agent, other sustained release agents and in ratio of the present invention
Composite slow release agent outside range, the present invention have more preferably control effect, show that its slow release effect is more excellent.
Security survey to capsicum is observed, each chemicals treatment of the present invention for several days after the 1st day and medicine after spray every time
Capsicum is occurred without poisoning phenomenon.
A kind of Bao Han famoxadones of the present invention and the agricultural bactericidal composition of hymexazol by specific example into
Description is gone, those skilled in the art can use for reference the content of present invention, and the links such as appropriate feed change, process conditions are corresponding to realize
Other purposes, correlation change all without departing from present disclosure, all similar substitutions and modifications are for this field skill
It is it will be apparent that being considered as being included within the scope of the present invention for art personnel.
Claims (10)
1. a kind of bactericidal composition of Han famoxadones and hymexazol, which is characterized in that the sterilization of the bactericidal composition is lived
It is 1 that property ingredient, which is weight ratio,:20-20:1 famoxadones and hymexazol.
2. bactericidal composition according to claim 1, which is characterized in that the weight ratio of the two is 1:5-5:1.
3. bactericidal composition according to claim 1, which is characterized in that can also include to be selected from the group in bactericidal composition
Third component：Triazolone, Triadimenol, metrafenone, Fluoxastrobin, captan or folpet；Suo Shu famoxadones, hymexazol and the
The weight ratio of three components is：1-10:1-10:1:1-3.
4. bactericidal composition , according to claim 3 it is characterized in that , famoxadones, hymexazol and third component weight
Amount is than being 1-5:1-5:1-2.
5. bactericidal composition , according to claim 3 it is characterized in that , famoxadones, hymexazol and third component weight
Amount is than being 3:3:1.
6. the sterilization sustained release agent of bactericidal composition according to any one of claims 1 to 5, which is characterized in that the sustained release agent
It is grouped as by following group by weight percentage：
Bactericidal composition：1-15%；
Sustained release agent：50-70%；
Hardening agent：10-30%；
Surplus is water；
The hardening agent is aluminum sulfate, copper sulphate or ferrous sulfate；
The sustained release agent is that weight ratio is 1:2-4:The composite slow release agent of 1 sodium bentonite and cyclodextrin composition.
7. sterilization sustained release agent according to claim 6, which is characterized in that the bactericidal composition is 8%.
8. sterilization sustained release agent according to claim 6, which is characterized in that the sustained release agent is 60%.
9. sterilization sustained release agent according to claim 6, which is characterized in that the sustained release agent is that weight ratio is 2:1 sodium base
The composite slow release agent of bentonite and cyclodextrin composition.
10. bactericidal composition according to any one of claims 1 to 5 or any sterilization sustained release agent of claim 6 to 9
Purposes for preventing cucumber downy mildew, the late blight of potato, watermelon blight or capsicum damping-off.
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| CN107980791A (en) * | 2017-12-14 | 2018-05-04 | 广东广康生化科技股份有限公司 | Containing captan He the bactericidal composition of famoxadone and its application |
| FR3096872A1 (en) * | 2019-06-05 | 2020-12-11 | UPL Corporation Limited | fungicidal composition for controlling zymoseptoria infection in plants |
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| WO2007031507A1 (en) * | 2005-09-13 | 2007-03-22 | Bayer Cropscience Ag | Fungicide composition comprising an arylamidine derivative and two known fungicide compounds |
| CN103918691A (en) * | 2013-01-12 | 2014-07-16 | 王学权 | Imazalil-containing sterilization composition |
| CN105052995A (en) * | 2015-07-31 | 2015-11-18 | 广西田园生化股份有限公司 | Compound composition containing Dufulin and oxazole bactericide, and preparation thereof |
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|---|---|---|---|---|
| WO2007031507A1 (en) * | 2005-09-13 | 2007-03-22 | Bayer Cropscience Ag | Fungicide composition comprising an arylamidine derivative and two known fungicide compounds |
| CN103918691A (en) * | 2013-01-12 | 2014-07-16 | 王学权 | Imazalil-containing sterilization composition |
| CN105052995A (en) * | 2015-07-31 | 2015-11-18 | 广西田园生化股份有限公司 | Compound composition containing Dufulin and oxazole bactericide, and preparation thereof |
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