CN105052959B - The complex composition of osthole and thifluzamide and its application - Google Patents
The complex composition of osthole and thifluzamide and its application Download PDFInfo
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- CN105052959B CN105052959B CN201510464215.3A CN201510464215A CN105052959B CN 105052959 B CN105052959 B CN 105052959B CN 201510464215 A CN201510464215 A CN 201510464215A CN 105052959 B CN105052959 B CN 105052959B
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- Prior art keywords
- osthole
- thifluzamide
- complex composition
- sharp eyespot
- wheat sharp
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- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 title claims abstract description 42
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical group C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 title claims abstract description 42
- WOSNCVAPUOFXEH-UHFFFAOYSA-N thifluzamide Chemical compound S1C(C)=NC(C(F)(F)F)=C1C(=O)NC1=C(Br)C=C(OC(F)(F)F)C=C1Br WOSNCVAPUOFXEH-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 239000000203 mixture Substances 0.000 title claims abstract description 32
- 241000209140 Triticum Species 0.000 claims abstract description 20
- 235000021307 Triticum Nutrition 0.000 claims abstract description 20
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 9
- 239000003899 bactericide agent Substances 0.000 abstract description 6
- 239000002131 composite material Substances 0.000 abstract description 6
- 239000004615 ingredient Substances 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 2
- 231100000053 low toxicity Toxicity 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 13
- 150000001875 compounds Chemical class 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 230000003449 preventive effect Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 244000052616 bacterial pathogen Species 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 5
- 210000000582 semen Anatomy 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 231100000419 toxicity Toxicity 0.000 description 4
- 230000001988 toxicity Effects 0.000 description 4
- 241001290235 Ceratobasidium cereale Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000417 fungicide Substances 0.000 description 3
- 235000010603 pastilles Nutrition 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 239000000375 suspending agent Substances 0.000 description 3
- 241001430066 Anulocaulis Species 0.000 description 2
- 241001530056 Athelia rolfsii Species 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 239000005822 Propiconazole Substances 0.000 description 2
- 206010039509 Scab Diseases 0.000 description 2
- 150000001408 amides Chemical group 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 239000004495 emulsifiable concentrate Substances 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- WCZBUQIZTSIQFE-UHFFFAOYSA-N furan;thiophene Chemical compound C=1C=COC=1.C=1C=CSC=1 WCZBUQIZTSIQFE-UHFFFAOYSA-N 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- STJLVHWMYQXCPB-UHFFFAOYSA-N propiconazole Chemical compound O1C(CCC)COC1(C=1C(=CC(Cl)=CC=1)Cl)CN1N=CN=C1 STJLVHWMYQXCPB-UHFFFAOYSA-N 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- -1 thiazole Acid amide Chemical class 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241001254604 Angelica pubescens Species 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000212948 Cnidium Species 0.000 description 1
- 241001529717 Corticium <basidiomycota> Species 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000702321 Microvirus Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 244000303512 Peucedanum ostruthium Species 0.000 description 1
- 235000007155 Peucedanum ostruthium Nutrition 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000221300 Puccinia Species 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 244000296102 Selinum monnieri Species 0.000 description 1
- 235000019084 Selinum monnieri Nutrition 0.000 description 1
- 241000722133 Tilletia Species 0.000 description 1
- 241000221566 Ustilago Species 0.000 description 1
- OYVABZRZDZPVQD-UHFFFAOYSA-N [F].C=1C=CSC=1 Chemical group [F].C=1C=CSC=1 OYVABZRZDZPVQD-UHFFFAOYSA-N 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 239000000890 drug combination Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000009781 safety test method Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical group [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Abstract
The present invention relates to a kind of good with environment compatibility, efficient, low toxicity, low-residual, the composite bactericide being made up of osthole and thifluzamide, it is particularly useful for the applied research of wheat sharp eyespot.In complex composition, the summation action ratio of osthole and two kinds of effective ingredient of thifluzamide is 1：1～1:32, it is had outstanding performance in terms of application preventing and treating wheat sharp eyespot.
Description
Technical field
The present invention relates to a kind of be applied to prevent and treat the antibacterial of wheat sharp eyespot, specifically, it is that one kind is applied to prevent and treat
The osthole of wheat sharp eyespot, thifluzamide composite bactericide.
Background technology
Wheat sharp eyespot, also known as sharp eye spot disease (wheat sharp eyespot), is that one kind generally occurs on Semen Tritici aestivi, endangers
The serious silborne fungal diseases of evil.It is reported first abroad within 1934, China finds have wheat seedling big in nineteen fifty-five in Jiangyin county
The withered phenomenon of area, but it was called Semen Tritici aestivi damping-off at that time, it is not taken seriously.Until 1973, wheat sharp eyespot was just again
Secondary it is realized.In recent years, because the establishing in large scale of susceptible variety, liquid manure apply the factor such as unreasonable, sheath blight fungus are led to
A large amount of accumulate, this disease generation region expands rapidly, has different degrees of generation in nearly 20 provinces and cities of China, especially with Jiangsu,
Anhui, Shandong, Henan, Hebei etc. save the area of wheat and occur generally and endanger to be on the rise, and the yield and quality of Semen Tritici aestivi is caused directly
The threat connecing, the lighter's production loss 5%～10% severe one underproduction 20%～40%, or even cause withered booting, withered dead ears, granule no
Receive.Banded sclerotial blight has become the important restriction factor of wheat increase yield.In a foreseeable future, chemical bactericide is still preventing and treating Semen Tritici aestivi disease
The Main Means of evil, but chemical pesticide a large amount of for a long time unreasonably using result in cost accounting increase, environmental pollution, Drug resistance
The problems such as there is development.Mixing chemical pesticide from botanical fungicide and reasonable field bucket is to solve the not science conjunction of current pesticide
The important channel that reason uses.
As the osthole of botanical fungicide, chemical name be 7- methoxyl group -8- (3 '-methyl -2 '-cyclobutenyl) -
1- dihydrobenzopyrans ketone -2, are a kind of from Umbelliferae annual herb plant cnidium monnieri (Cnidium monnieric (L.)
Cuss. plant extract), exists in Imperatoria ostruthium, circle Radix Angelicae Sinensis, Angelica pubescens, the root of bolt wing celery, in rhizome, has multiple maturations
Extracting method, originally apply medically, later agriculturally can applying as insecticidal bactericide, but osthole and thiophene furan
The complex composition of amide have not been reported in terms of preventing and treating wheat sharp eyespot.Thifluzamide is thiophene fluorine bacterium amine again, belongs to thiazole
Acid amide fungicides, have strong Uptake and translocation and long lasting effect.Thifluzamide to Rhizoctonia, Puccinia, Ustilago,
The pathomycetes such as Tilletia, corticium, nuclear cavity Pseudomonas are all active；Thifluzamide is succinate dehydrogenation enzyme level
Agent, its mechanism of action is succinic acid dehydrogenase in suppression pathogenic bacteria tricarboxylic acid cycle, leads to thalline dead.
Content of the invention
For the important disease of effective prevention and control Semen Tritici aestivi, reduce the field rate of chemical bactericide, the present invention is in prior art
On the basis of, by furtheing investigate the application in agriculture field of osthole and thifluzamide complex composition, in particular for
The study on prevention of wheat sharp eyespot, there is provided a kind of osthole of preventing and treating wheat sharp eyespot, thifluzamide single dose and its compounding
Compositionss；This complex composition is to be compounded osthole with thifluzamide, through indoor and outdoor tests, has filtered out compounding killing
The optimum proportioning of microbial inoculum, potentiation is notable, is advantageously implemented and reduces environmental pollution, controls pathogenic bacterium resistance that the purpose of development occurs.
Osthole and the complex composition of thifluzamide, are characterized in that, its effective component of osthole and thiophene furan
The weight proportion of amide is 1：1～1：32；
Further, the potentiation weight proportion of osthole and thifluzamide is 1：8～1：16；
Further, the optimum weight ratio of osthole and thifluzamide is 1:8, potentiation system during optimum compound proportion example
Number is 2.04.
Osthole, two kinds of antibacterial of thifluzamide show good inhibitory action to sheath blight fungus, osthole and
Thifluzamide composite usage is prevented and treated banded sclerotial blight and is shown obvious potentiation.There is following advantage：
1st, there is potentiation.
Toxicity Determination result shows, osthole, thifluzamide are to both rhizoctonia cerealis 1:8 to 1:16 models
Potentiation is assumed, wherein with 1 in enclosing:8 potentiation is the most notable, and coefficient of synergism reaches 2.04.
2nd, optimum proportioning field bucket mixes preventive effect and projects, and decreases preparation consumption.
9% osthole thifluzamide complex composition 45,54g/ha make in field control wheat sharp eyespot in our hospital
The test of pesticide effectiveness in have outstanding performance, better than or be equivalent to the prevention effect of single dose, comparison medicament.Meanwhile, medicament being greatly decreased has
Effect Ingredient Amount, with directly according to label concentration bucket mixed phase ratio, complex composition 45,54g/ha reduce effective ingredient consumption respectively
40.79%th, 28.95%.
3rd, high security.
Osthole, thifluzamide are low to person poultry toxicity, and the complex composition that our hospital is obtained effectively becomes less than market single dose
The content divided, safer to crop, environment and eater.
4th, reduce the risk of pathogenic bacterium Drug resistance generation.
As described in advantage 2,3, the consumption of complex composition osthole and thifluzamide is significantly lower than city and sells single-dose product,
And field usage amount significantly reduces, this can be greatly reduced the selection pressure to both antibacterial for the wheat sharp eyespot pathogenic bacterium.This
Outward, osthole, the thifluzamide mechanism of action are different, and bucket mixes to use and is less susceptible to make pathogenic bacterium develop immunity to drugs.
Specific embodiment
The present invention is described in detail with following instantiation, but limitation of the present invention absolutely not.Percentage in all proportionings
It is weight percentage than all.
Embodiment 1
Toxicity Determination is screened with compound proportion
1.1 reagent agent
94.7% osthole active compound, academy of agricultural sciences of Jiangsu Province Safety of Food Quality institute provides.
96% thifluzamide active compound, Shandong Province Zhaoyuan three Far East Chemical Co., Ltd. product.
1.2 strains tested
Experiment target is designated as wheat sharp eyespot (Rhizoctonia cerealis Vander Hoeven) pathogenic bacteria bacterial strain sample
Separate from the old complaint stem of field and obtain, the first generation bacterial strain that culture purified obtains, save backup in 4 DEG C of refrigerators.
1.3 instrument and equipment
Mainly there are electronic balance, superclean bench, bio-incubator, culture dish, micropipettor etc..
1.4 experimental technique
The biomass growth rate method (Mycelium growth rate test) recommended using FAO.
1.4.1 composite reagent
Table 1 osthole and thifluzamide compound proportion
Table 2 compound drug diluted concentration gradient
1.4.2 the preparation of pastille flat board
The drug concentration gradient setting according to 1.4.1, medicament is added in the quantitative PDA culture medium dissolved, makes pastille
Flat board, with not pastille flat board as blank, every concentration is repeated 3 times.
1.4.3 connecing bacterium culture
Beaten along cultured colony edge with diameter 0.6 card punch and take bacterium disk, with sterilization Inoculating needle, bacterium disk is inverted and move
Plant on flat board, constant temperature culture 5 days under the conditions of being placed in 25 DEG C.
1.4.4 result investigation
Measurement method measurement bacterium colony extension diameter is intersected with ruler, compared with not dispenser comparison bacterium disk extension diameter, calculates
The suppression ratio to mycelial growth for the medicament.
1.5 statistical analysiss
Regression analyses are made according to each drug concentration logarithm value and corresponding mycelial growth inhibition rate niqueMin, calculates each medicament
Virulence regression equation, correlation coefficient (r) and EC50.
When carrying out drug combination toxicity test, calculate the coefficient of synergism (SR) of complex composition according to Wadley method, evaluate
The synergy type of complex composition.Evaluate the potentiation of composite reagent, i.e. SR according to coefficient of synergism (SR)<0.5 is
Antagonism, 0.5≤SR≤1.5 are summation action, SR>1.5 is potentiation.Coefficient of synergism (SR) presses formula (1), (2) calculate：
In formula：
X1----complex composition EC50Theoretical value, unit is mg/L；
PA--- the percentage composition of A in-complex composition, unit is percentage rate (%)；
PB--- the percentage composition of B in-complex composition, unit is percentage rate (%)；
The EC of A----A50Value, unit is mg/L；
The EC of B----B50Value, unit is mg/L.
In formula：
The coefficient of synergism of SR---- complex composition；
X1----complex composition EC50Theoretical value, unit is mg/L；
X2----complex composition EC50Measured value, unit is mg/L.
1.6 interpretation of result
Indoor optimum compound proportion example test result indicate that, osthole, both thifluzamides 1:2、1:4、1:32 have phase
Plus effect, 1:8、1:16 have a potentiation, and wherein 1:8 potentiation is the most notable, and coefficient of synergism is 2.04, for most preferably multiple
Proportioning example.
Table 3 osthole and thifluzamide suppression rhizoctonia cerealis optimum compound proportion example screening
Embodiment 2
Field plot trial
Osthole is filtered out according to indoor compound proportion screening experiment:Thifluzamide=1:8 is optimum proportioning, Wo Menpei
Make 9% osthole thifluzamide complex composition.According to the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC chief editor's《Pesticide field efficacy tries
Test criterion (two)》13rd, 121,181 parts design 9% osthole thifluzamide complex composition to wheat sharp eyespot
Field control effectiveness test.
2.1 reagent agent
240 g/l of thifluzamide suspending agent 54g/ha, Japanese Nissan chemical industry Co., Ltd.；
1% osthole water-emulsion agent 22g/ha, Chengdu Lvjin biotechnology Co., Ltd.
9% osthole thifluzamide 36,45,54g/ha, Yantai academy of agricultural sciences prepares.
2.2 comparison medicament
250 g/l of propiconazole emulsifiable concentrate 150g/ha, Xian Zhengda crop protection company limited of Switzerland product.
2.3 Quarter Design
Test sets 7 process altogether, often processes 4 repetitions, sets 28 cells, every cell 50m altogether2.Each cell random alignment.
Spraying equipment adopts MATABI SuperGreen 16 type knapsack sprayer, injection diameter 0.8mm, operating pressure 2.0 bar.Spray
Make during mist leave dual sides uniformly medicine, till dripping.
2.4 investigation method
0 grade：Anosis；
1 grade：Sheath is fallen ill but cane is not fallen ill；
3 grades：Sheath is fallen ill, and invades stem, but cane scab ring stem is less than 1/2；
5 grades：Cane scab ring stem is more than 1/2, but non-lodging or fracture；
7 grades：Withered, lodging, withered dead ears.
2.5 drug effect computational methods
2.6 result of the test
9% osthole thifluzamide complex composition 36,45,54g/ha preventive effect be respectively 72.26%, 75.80%,
81.44%, it is significantly higher than the preventive effect of 1% osthole water-emulsion agent 22g/ha, 9% osthole thifluzamide complex composition
54g/ha preventive effect and 240g/L thifluzamide suspending agent 54g/ha no significant difference；250 g/l of propiconazole emulsifiable concentrates of comparison medicament
The preventive effect of 150g/ha is suitable with the preventive effect of 9% osthole thifluzamide complex composition 45g/ha, but substantially less than 54g/
The preventive effect of ha and 240g/L thifluzamide suspending agent 54g/ha.
Table 4 9% osthole thifluzamide built composition for preventing and controlling wheat sharp eyespot field efficacy
Embodiment 3
Safety testing
In complex composition, osthole and thifluzamide content are less than the content of commercially available prod, therefore its poison to people and animals
Property is satisfactory with the safety of crop.Composite bactericide shows to belong to micro-virus kind agriculture through assert mechanism's toxicity test result
Medicine：
Rat acute toxicity♀>5000mg/kg；
Rat acute percutaneous toxicity♀>20000mg/kg.
Claims (4)
1. the complex composition of osthole and thifluzamide, is characterised by
The weight proportion of its effective component of osthole and thifluzamide is 1：8～1：16.
2. the complex composition of osthole as claimed in claim 1 and thifluzamide, is characterised by
The optimum weight ratio of osthole and thifluzamide is 1:8.
3. the complex composition of osthole and thifluzamide described in any one of claim 1-2 is applied to prevent and treat wheat sharp eyespot
Purposes.
4. purposes as claimed in claim 3 is prevented it is characterised in that making 9% osthole thifluzamide complex composition
Control wheat sharp eyespot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510464215.3A CN105052959B (en) | 2015-07-31 | 2015-07-31 | The complex composition of osthole and thifluzamide and its application |
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| CN201510464215.3A CN105052959B (en) | 2015-07-31 | 2015-07-31 | The complex composition of osthole and thifluzamide and its application |
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| CN1086909C (en) * | 1999-04-30 | 2002-07-03 | 湖南农业大学 | Preparation method of fungicide for controlling banded sclerotial blight |
| CN1119930C (en) * | 2001-08-08 | 2003-09-03 | 江苏省农业科学院 | Application of cnidiadin in agriculture |
| RU2017108785A (en) * | 2011-06-24 | 2019-01-22 | Доу Агросаенсиз Ллк | PESTICIDAL COMPOSITIONS AND RELATED WAYS |
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Denomination of invention: Compound composition of Osthol and thiafuramide and its application Effective date of registration: 20211123 Granted publication date: 20170301 Pledgee: Yantai Fushan huiran Private Capital Management Co.,Ltd. Pledgor: SHANDONG YANTAI AGRICULTURAL SCI & TECH INSTITUTE Registration number: Y2021980012999 |
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