CN107637632A - A kind of three high crowd special biscuit and its preparation for processing - Google Patents
A kind of three high crowd special biscuit and its preparation for processing Download PDFInfo
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Abstract
The invention discloses special biscuit of a kind of three high crowds and preparation method thereof, including Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, resistant dextrin, egg, vegetable oil, skimmed milk power, raising agent, flavor enhancement.Compared with prior art, Radix et Rhizoma Gynurae divaricatae biscuit of the invention obtains a kind of sugar and the low biscuit of fat content, not only mouthfeel is good for the biscuit, and contains reducing blood sugar and blood fat composition using special Radix et Rhizoma Gynurae divaricatae extract as major ingredient.
Description
Technical field
The present invention relates to food processing technology field, more particularly to a kind of special biscuit of three high crowd and its
Preparation for processing.
Background technology
Radix et Rhizoma Gynurae divaricatae is composite family pseudo-ginseng platymiscium, also known as Radix et Rhizoma Gynurae divaricatae, Bai Dongfeng, beautiful loquat, asiatic toddalia root-bark, thick musculus cutaneus, chicken
Dish, big beef fat, white kind of amaranth etc., it is distributed in TaiWan, China to south China, a southwestern generation, happiness and is born on the shaded place of humidity.Its rhizome fertilizer
Greatly, meat, in lumps, footpath 2-3cm, lark when fresh, more pieces of the raw stem eye in side.
Radix et Rhizoma Gynurae divaricatae the whole province can be used as medicine, its acrid flavour, light；It is mild-natured, there is heat-clearing, relaxing muscles and tendons, hemostasis, dissolving stasis and other effects, energy
Enough treat the functions such as pertussis, rheumatalgia, fracture, traumatic bleeding, hypoglycemic, pulmonary tuberculosis.Contain flavones, flavones in Radix et Rhizoma Gynurae divaricatae
Internal oxygen radical can be effectively removed, improves blood circulation, cholesterol is reduced, blood glucose and ternary aliphatic acid third can also be reduced
Ester content, there is the effect of hypoglycemic, hypotensive, anti-oxidant, anti-aging and other effects, and Quercetin has hypotensive, antioxygen
Bioactivity, the Radix et Rhizoma Gynurae divaricatae medical values such as change, anti-inflammatory, antitumor and its regulation immunologic function are notable.
Obesity is a kind of state of adipose tissue excess accumulation in vivo, at present typically using body mass index BMI [body weight/
(height)2] judge obesity.BMI is defined as obesity by WHO more than 30, and more than 25 be overweight；China is fertilizer with BMI >=28
Fat, 24≤BMI ＜ 28 are overweight.With the development of society, people's living standard improves, the quantity of obese people is constantly soaring.
WHO data shows that global overweight people's quantity increases by 1 times in every 5 years.General Administration of Sport's national physique detection publication shows within 2010,
Rate that China adult is overweight is 32.1%, obesity rates 9.9%；The overweight rate and obesity rates of 60-69 year the elderly, also slightly above into
Year people；In addition, the body shape index such as the height of 3-6 year child, body weight, bust, is in rise appreciably trend, wherein amplification most
Big is body weight.Obesity has evolved into the formidable enemy for influenceing health of people, and it is listed as with AIDS, malicious anaesthetic addiction and alcoholophilia
The big medical science social concern in the world four.
Existing biscuit is in order to meet the taste of people, and the content of sugar and grease is very high in biscuit, be not suitable for hyperglycaemia and
People with hyperlipidemia is eaten, and excessive eat also results in obesity, has certain adverse effect to the health of people.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide the special biscuit of a kind of three high crowds and its add
Work preparation method.
Compared with prior art, technical scheme is as follows：A kind of special biscuit of three high crowd, its component and its
Parts by weight include：
Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder 70-80 parts；
Resistant dextrin 0.5-1 parts；
Egg 1-2 parts；
Vegetable oil 15-20 parts；
Skimmed milk power 1-2 parts；
Raising agent 0.5-1 parts；
Flavor enhancement 0.5-1 parts.
As the further optimization of such scheme, the preparation method of the Radix et Rhizoma Gynurae divaricatae extract, comprise the following steps：
(21) divaricate velvetplant leaf is sheared, is cut after natural air drying to 3mm fritters, crushed 30 mesh sieves；According to feed liquid
Than 1:40 ratios add 50% volume fraction ethanol, soak 120min, obtain first time Flavonoid extract；By first time Flavonoid extract
It is positioned over phase analysis in circulation reflux device, first time refluxing extraction is carried out under the conditions of 60 DEG C, flows back 90min；Returned in first time
After stream extraction, medicinal extract and phase analysis are positioned over power to extract 2.5min under 360W microwaves；Enter after Microwave Extraction and return next time
Stream extraction；After refluxing extraction 2 times, stop refluxing extraction and obtain medicinal extract, be filtered under diminished pressure 40min to crude drug amount 0.1g/L, obtain white
Carry on the back pseudo-ginseng supernatant extract solution；
(22) polyamide is weighed according to 2 times of mass ratioes of medicinal material, boils polyamide 2 times, each 1h with ethanol,
Wet method upper prop, it is 1 to obtain resin blade diameter length ratio:8；The concentrated extract pH3.0 that regulating step (5) obtains, with less than 4BV/h flow velocitys
Concentrated extract is added dropwise in polyamide, eluted by 70% ethanol of pH3.0 mass concentrations as eluent, 3BV/h
Detected to spectrophotometer and Flavonoid substances are not present in eluent, obtain Radix et Rhizoma Gynurae divaricatae precipitation extract；
(23) sodium gluconate and the 1-3 times of white back of the body three of 1 times of Radix et Rhizoma Gynurae divaricatae precipitation quality are added in Radix et Rhizoma Gynurae divaricatae precipitation
The trisodium citrate of seven precipitation quality, with lemon acid for adjusting pH to 6.0-7.0, is incubated 2-8h, through separation of solid and liquid at 80-100 DEG C
Afterwards, liquid portion is concentrated 2-3 times, the ethanol for adding 1-2 times of volume carries out alcohol precipitation, through separation of solid and liquid, retains solid portion, obtains
Obtain Radix et Rhizoma Gynurae divaricatae precipitation extract；
(24) the Radix et Rhizoma Gynurae divaricatae supernatant extract solution of step (22) is mixed with the Radix et Rhizoma Gynurae divaricatae precipitation extract of step (23)
Homogenate is broken into, through vacuum freeze drying, obtains Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder.
As the further optimization of such scheme, in the step (21), the Radix et Rhizoma Gynurae divaricatae blade in collection 8-10 months is made
For raw material.
As the further optimization of such scheme, the one kind of the vegetable oil in palm oil, corn oil, sunflower oil
Or a variety of mixtures.
As the further optimization of such scheme, one or more of the flavor enhancement in salt, essence, ground coffee are mixed
Compound.
As the further optimization of such scheme, the divaricate velvetplant leaf is prepared via a method which to form：
(11) tissue cultures：Radix et Rhizoma Gynurae divaricatae blade is taken under aseptic technique, to be connected to as explant with vacuum side of blade
Improve in MS inducing cultures, induction differentiation obtains callus, and for light culture after 8-10 days, it is 1000- to be transferred to intensity of illumination
In 1500Lx culturing room, humidity 80-85% is kept, is cultivated 8-10 days；Squamous subculture 2-3 transfers in differential medium after generation,
Wherein, differential medium is 0.2-0.4mg/L methyl α-naphthyl acetate, the 1/2MS culture medium of 2-5% mass fraction sucrose；1500-
Under 2000Lx intensities of illumination, cultivate 20-30 days, obtain test tube seedling；
(12) water planting：Test tube seedling is inoculated in water planting liquid, wherein, MnSO4,6.0- added with 40-45mg/L
7.0mg/L Zn SO4；Under natural light, cultivate 8-10 days, obtain Radix et Rhizoma Gynurae divaricatae seedling；
(13) greenhouse gardening：According to turf：Vermiculite：Fine sand is by volume (3-5):(0.5-1.5):(0.5-1.5) is prepared
Mixed culture medium；By Radix et Rhizoma Gynurae divaricatae seedling cultivation in mixed culture medium, under natural light, after cultivating 5-8 days, according to 1500-
2000kg/ mus apply decomposed manure；Divaricate velvetplant leaf is harvested after 4-5 months；Ventilation in good time, irrigate.
Present invention also offers a kind of preparation of the special biscuit of a kind of three high crowds as described in claim 1-6 is any
Method, it is characterised in that comprise the following steps：
(I) Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, resistant dextrin, egg, vegetable oil, degreasing are sequentially added according to formula rate
Milk powder, raising agent, flavor enhancement, often adding a kind of component will stir, and add water during stirring on demand, obtain mixing dough；
(II) semi-finished product are made according to conventional biscuit forming method in mixing dough, the Radix et Rhizoma Gynurae divaricatae is obtained after baked
Biscuit finished product.
The present invention principle be：The present invention is based on the abundant nutriment contained in Radix et Rhizoma Gynurae divaricatae, the main dialogue back of the body three
Flavonoid substances and soluble dietary fiber in seven are extracted and concentrated.Wherein Radix et Rhizoma Gynurae divaricatae flavones can reduce blood glucose
With ternary aliphatic propyl propionate content, there is hypoglycemic, hypotensive, anti-oxidant, anti-aging and other effects；Soluble dietary fiber has
There is very strong water absorption and swelling performance, swelling volume and weight increase 10-15 times after water suction, can increase the satiety of people, and not
Heat can be provided to avoid heat energy excessively raw and cause building up for body fat, be advantageous to healthy weight reducing；Resistant dextrin is except can
Increase the fiber content of food, also have and the characteristics of heat is lasting is provided, intake resistant dextrin can cause hypoglycemia, and (glucose is anti-
Should=25) and blood insulin react (insulin response=13), therefore as the carbohydrate slowly to release energy, it can be with
Partially or completely replace other carbohydrate, it helps postpone producing again for hunger, reduce human body sugar and fatty and take the photograph
Enter amount, be advantageous to lose weight.
The present invention has advantages below compared with prior art：
1st, the invention provides special biscuit of a kind of three high crowds and preparation method thereof, the Radix et Rhizoma Gynurae divaricatae biscuit is with special
Radix et Rhizoma Gynurae divaricatae extract be major ingredient, obtain it is a kind of sugar and the low biscuit of fat content, not only mouthfeel is good for the biscuit, and
Containing reducing blood sugar and blood fat composition, there is certain effect of weight reducing, compensate for the technological deficiency that edible biscuits are easily caused obesity.
2nd, the invention provides the special biscuit of a kind of three high crowds, the Radix et Rhizoma Gynurae divaricatae extract of use, from Radix et Rhizoma Gynurae divaricatae
Growth pattern improves, processing is extracted optimization etc. and set about, and effectively improves Radix et Rhizoma Gynurae divaricatae flavones pick-up rate.
3rd, the invention provides the special biscuit of a kind of three high crowds, for the Radix et Rhizoma Gynurae divaricatae extract of use, conjunction is passed through
Hormone, nutrient needed for reason selection tissue culture procedures so that final test tube seedling general flavone content brings up to 3.9mg/
G, and raising of the accumulation of test tube sprout total flavone effective dose to white-backed pseudo-ginseng total flavonoid content after maturation is made with important promotion
With.
4th, it is creative for the Radix et Rhizoma Gynurae divaricatae extract of use the invention provides the special biscuit of a kind of three high crowds
The water planting hardening for nascent test tube seedling progress 8-10 days that ground obtains tissue cultures, reasonable disposition Mn2+, Zn2+, Ca (NO3) 2,
(NH4) 2SO4 so that final ripe white-backed pseudo-ginseng total flavonoid content brings up to 11.8, and increase ratio is 640%.
5th, the invention provides the special biscuit of a kind of three high crowds, for the Radix et Rhizoma Gynurae divaricatae extract of use, improving
On the basis of Radix et Rhizoma Gynurae divaricatae plantation efficiency and quality, each Parameters variation in Radix et Rhizoma Gynurae divaricatae extraction process is also rationally controlled, is not only grasped
Make simple and improve 2-4% percentage points relative to traditional recovery rate 6-8%, have to scale extraction production important
Economic value.With reference to polyamide purge process, whole white-backed pseudo-ginseng total flavonoid pick-up rate can reach more than 11%, and purity surpasses
Cross 35%.
Embodiment
The invention provides the special biscuit of a kind of three high crowds, its component and its parts by weight include：
Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder 60-80 parts
Resistant dextrin 0.5-1 parts
Egg 1-2 parts
Vegetable oil 15-20 parts
Skimmed milk power 1-2 parts
Raising agent 0.5-1 parts
Flavor enhancement 0.5-1 parts
Wherein, the preparation method of the Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, comprises the following steps：
The preparation method of the Radix et Rhizoma Gynurae divaricatae extract, comprises the following steps：
The preparation method of the Radix et Rhizoma Gynurae divaricatae extract, comprises the following steps：
(21) divaricate velvetplant leaf is sheared, is cut after natural air drying to 3mm fritters, crushed 30 mesh sieves；According to feed liquid
Than 1:40 ratios add 50% volume fraction ethanol, soak 120min, obtain first time Flavonoid extract；By first time Flavonoid extract
It is positioned over phase analysis in circulation reflux device, first time refluxing extraction is carried out under the conditions of 60 DEG C, flows back 90min；Returned in first time
After stream extraction, medicinal extract and phase analysis are positioned over power to extract 2.5min under 360W microwaves；Enter after Microwave Extraction and return next time
Stream extraction；After refluxing extraction 2 times, stop refluxing extraction and obtain medicinal extract, be filtered under diminished pressure 40min to crude drug amount 0.1g/L, obtain white
Carry on the back pseudo-ginseng supernatant extract solution；
(22) polyamide is weighed according to 2 times of mass ratioes of medicinal material, boils polyamide 2 times, each 1h with ethanol,
Wet method upper prop, it is 1 to obtain resin blade diameter length ratio:8；The concentrated extract pH3.0 that regulating step (5) obtains, with less than 4BV/h flow velocitys
Concentrated extract is added dropwise in polyamide, eluted by 70% ethanol of pH3.0 mass concentrations as eluent, 3BV/h
Detected to spectrophotometer and Flavonoid substances are not present in eluent, obtain Radix et Rhizoma Gynurae divaricatae precipitation extract；
(23) sodium gluconate and the 1-3 times of white back of the body three of 1 times of Radix et Rhizoma Gynurae divaricatae precipitation quality are added in Radix et Rhizoma Gynurae divaricatae precipitation
The trisodium citrate of seven precipitation quality, with lemon acid for adjusting pH to 6.0-7.0, is incubated 2-8h, through separation of solid and liquid at 80-100 DEG C
Afterwards, liquid portion is concentrated 2-3 times, the ethanol for adding 1-2 times of volume carries out alcohol precipitation, through separation of solid and liquid, retains solid portion, obtains
Obtain Radix et Rhizoma Gynurae divaricatae precipitation extract；
(24) the Radix et Rhizoma Gynurae divaricatae supernatant extract solution of step (22) is mixed with the Radix et Rhizoma Gynurae divaricatae precipitation extract of step (23)
Homogenate is broken into, through vacuum freeze drying, obtains Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder.
One or more mixtures of the vegetable oil in palm oil, corn oil, sunflower oil.
One or more mixtures of the flavor enhancement in salt, essence, ground coffee.
The preparation method of the Radix et Rhizoma Gynurae divaricatae biscuit, comprises the following steps：
(I) Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, resistant dextrin, egg, vegetable oil, degreasing are sequentially added according to formula rate
Milk powder, raising agent, flavor enhancement, often adding a kind of component will stir, and add water during stirring on demand, obtain mixing dough；
(II) semi-finished product are made according to conventional biscuit forming method in mixing dough, the Radix et Rhizoma Gynurae divaricatae is obtained after baked
Biscuit finished product.
Below premised on techniques described above scheme, technical scheme is made specifically in conjunction with specific embodiments
It is bright, but protection scope of the present invention is not limited to following embodiments.
Embodiment 1
1st, material
The present embodiment method therefor is known to those skilled in the art the conventional method of dawn unless otherwise instructed, used
The material such as reagent, unless otherwise instructed, be commercially available products.
2nd, method
The preparation of 2.1 Radix et Rhizoma Gynurae divaricataes, comprises the following steps：
(1) culture medium is prepared：Prepare improvement MS inducing cultures：30g/L sucrose is added, agar content 8%, adjusts pH
For 5.5-6,1.5mg/L 6-BA, 0.1mg/L NAA, 1.3mg/L PVP are added；Prepare differential medium：2% mass point
Number sucrose, add 0.2mg/L NAA；Prepare mill water culture nutrient solution preparation：Nutrient composition includes following 28g/L sucrose, Ca
(NO3)20.07g/L；(NH4)SO4 0.2g/L；KH2PO4 1g/L, CaCl22H2O 5g/L, KCl 5g/L, MgSO4 2g/
L；H3BO3 0.046g/L；MnCl2·4H2O 0.04g/L；ZnSO4·7H2O 0.06g/L；CuSO4·5H2O 0.003g/
L；H2MoO4H2O 0.001g/L, 1mL Fe-EDTA solution is added in every liter of nutrient solution, distilled water is prepared, and regulation pH is
5.8-6.2。
(2) tissue cultures：In April, health is taken to obtain its young piece without insect pest Radix et Rhizoma Gynurae divaricatae, clean, cut blade edge, obtain
Obtain 1*1cm explants；Under aseptic technique, Radix et Rhizoma Gynurae divaricatae explant is placed in inducing culture, light culture 8 days, kept
Humidity 80%, after the generation of squamous subculture 2, it is transferred in differential medium, is cultivated 20 days under 2000Lx intensities of illumination, obtains test tube seedling.
(3) water planting：Above-mentioned test tube seedling is inoculated in mill water culture nutrient solution, under natural light, cultivates 10 days, obtains Radix et Rhizoma Gynurae divaricatae
Seedling.
(4) greenhouse gardening：By turf, vermiculite and fine sand according to volume ratio 3:0.5:0.5 is configured to mixed culture medium, will be white
Pseudo-ginseng seedling cultivation is carried on the back in mixed culture medium, natural optical culture is after 8 days, and decomposed manure is applied according to 2000kg/ mus, 2
Second of decomposed manure is applied according to 1500kg/ mus after month, is harvested after 3 months.
2.2 the preparation of Radix et Rhizoma Gynurae divaricatae extract
(21) divaricate velvetplant leaf in October is gathered, divaricate velvetplant leaf is sheared, is cut after natural air drying small to 3mm
Block, it crushed 30 mesh sieves；According to solid-liquid ratio 1:40 ratios add 50% volume fraction ethanol, soak 120min, obtain for the first time
Flavonoid extract；First time Flavonoid extract and phase analysis are positioned in circulation reflux device, first time backflow is carried out under the conditions of 60 DEG C
Extraction, flow back 90min；After first time refluxing extraction, medicinal extract and phase analysis are positioned over power to be extracted under 360W microwaves
2.5min；Enter refluxing extraction next time after Microwave Extraction；After refluxing extraction 2 times, stop refluxing extraction and obtain medicinal extract, depressurized
40min to crude drug amount 0.1g/L is filtered, obtains Radix et Rhizoma Gynurae divaricatae supernatant extract solution；
(22) polyamide is weighed according to 2 times of mass ratioes of medicinal material, boils polyamide 2 times, each 1h with ethanol,
Wet method upper prop, it is 1 to obtain resin blade diameter length ratio:8；The concentrated extract pH3.0 that regulating step (21) obtains, with less than 4BV/h flow velocitys
Concentrated extract is added dropwise in polyamide, eluted by 70% ethanol of pH3.0 mass concentrations as eluent, 3BV/h
Detected to spectrophotometer and Flavonoid substances are not present in eluent, obtain Radix et Rhizoma Gynurae divaricatae precipitation extract；
(23) sodium gluconate and the 1-3 times of white back of the body three of 1 times of Radix et Rhizoma Gynurae divaricatae precipitation quality are added in Radix et Rhizoma Gynurae divaricatae precipitation
The trisodium citrate of seven precipitation quality, with lemon acid for adjusting pH to 6.0-7.0, is incubated 2-8h, through separation of solid and liquid at 80-100 DEG C
Afterwards, liquid portion is concentrated 2-3 times, the ethanol for adding 1-2 times of volume carries out alcohol precipitation, through separation of solid and liquid, retains solid portion, obtains
Obtain Radix et Rhizoma Gynurae divaricatae precipitation extract；
(24) the Radix et Rhizoma Gynurae divaricatae supernatant extract solution of step (22) is mixed with the Radix et Rhizoma Gynurae divaricatae precipitation extract of step (23)
Homogenate is broken into, through vacuum freeze drying, obtains Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder.
The preparation of 2.3 Radix et Rhizoma Gynurae divaricatae biscuits
(I) by weight, raw material is weighed according to formula and number as follows：
60 parts of Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder；
0.5 part of resistant dextrin；
2 parts of egg；
20 parts of vegetable oil；
2 parts of skimmed milk power；
0.5 part of raising agent；
1 part of flavor enhancement；
Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, resistant dextrin, egg, vegetable oil, skimmed milk power, loose is sequentially added in order
Agent, flavor enhancement, often adding a kind of component will stir, and add water during stirring on demand, obtain mixing dough；
(II) semi-finished product are made according to conventional biscuit forming method in mixing dough, the Radix et Rhizoma Gynurae divaricatae is obtained after baked
Biscuit.
3rd, result verification
Influence of 3.0 culture mediums to Radix et Rhizoma Gynurae divaricatae yield of flavone
(1) influence of the inducing culture to Radix et Rhizoma Gynurae divaricatae yield of flavone
Preparing includes the improvement MS inducing cultures of different proportion hormone, as described in Table 1, in kind carries out induction training
Support, differentiation culture, using ponkan fourth as standard items after 30 days, general flavone amount in test tube seedling determined by ultraviolet specrophotometer,
As shown in table 2, in all improvement MS inducing cultures, 6-BA, NAA added with scope of the present invention, test tube sprout total flavone
Content is up to 3.6mg/g, as shown in table 1.
Influence of the different improvement MS inducing cultures of table 1 to test tube seedling general flavone content
(2) influence of differential medium Radix et Rhizoma Gynurae divaricatae yield of flavone
Preparing includes the 1/2MS differential mediums of different proportion NAA, sucrose, as described in Table 2, with 1.5mg/L, 0.1mg/
LNAA, 1.3mg/LPVP improvement MS inducing culture inductions are into callus, with above-mentioned 1/2MS differential mediums in the same terms
Lower differentiation culture, using ponkan fourth as standard items after 30 days, general flavone amount in test tube seedling is determined by ultraviolet specrophotometer, such as
Shown in table 2, under the conditions of all 1/2MS differential mediums, 6-BA, NAA added with scope of the present invention, test tube seedling is always yellow
Ketone content is up to 3.9mg/g, as shown in table 2.
Influence of the different 1/2MS differential mediums of table 2 to test tube seedling general flavone content
2. the influence of cultivating condition Radix et Rhizoma Gynurae divaricatae yield of flavone
(1)Mn2+、Zn2+Influence to Radix et Rhizoma Gynurae divaricatae yield of flavone
Inducing culture is used as using 1.5mg/L, 0.1mg/LNAA, 1.3mg/LPVP improvement MS；With 0.2mg/L, 2% matter
Measure fraction sucrose 1/2MS culture mediums and obtain test tube seedling as differential medium.Different water planting liquid are prepared, are entered with same culture conditions
Row culture, is transferred to greenhouse and is planted May, harvesting in October, general flavone amount is determined with ultraviolet specrophotometer, such as table 3, MnSO4、
ZnSO4In scope provided by the invention, flavones content can reach 11.8mg/g, and exceed the scope, and flavones content is equal
Have and decline by a relatively large margin.
Table 3Mn2+、Zn2+Influence to white-backed pseudo-ginseng total flavonoid content
(2) it is different to supply influence of the nitrogen level to Radix et Rhizoma Gynurae divaricatae yield of flavone
0.05g/L is prepared respectively：0.20g/L；0.06g/L:0.19g/L；0.07g/L:0.28g/L；0.09 g/L:
0.26g/L；0.15g/L:0.60g/L；0.01g/L:0.04g/L mass ratio Ca (NO3)2：(NH4)2SO4；Other conditions are identical, with
Ultraviolet specrophotometer determines general flavone amount, such as table 4.
Table 4 is different to supply influence of the nitrogen level to white-backed pseudo-ginseng total flavonoid content
3. extracting flavonoids procedure parameter com-parison and analysis
(1) influence of the Different Extraction Method to white-backed pseudo-ginseng total flavonoid yield
White-backed pseudo-ginseng total flavonoid extraction is carried out with microwave method, circumfluence method and microwave of the present invention+circumfluence method respectively
Experiment, by absorbance measuring, microwave method general flavone yield is 7.4%, circumfluence method general flavone yield is 9.1%, microwave+return
Stream method white-backed pseudo-ginseng total flavonoid yield is 10.1%, it is seen that it is always yellow to carry out Radix et Rhizoma Gynurae divaricatae with microwave provided by the invention+circumfluence method
Ketone extraction can largely improve general flavone yield.
(2) soak time influences on white-backed pseudo-ginseng total flavonoid yield before extracting
With 10 minutes for grade, 30-180min is set respectively, ethanol soak time parallel test, entered with backflow+microwave method
Row white-backed pseudo-ginseng total flavonoid recovery rate is tested, by absorbance measuring, as ethanol soak time extends, final general flavone yield
The 9.8% of 100 minutes is brought up to from 3.4%, subsequent general flavone yield starts slowly to decline, under general flavone yield during 180min
It is down to 8.7%.
(3) concentration of alcohol influences on white-backed pseudo-ginseng total flavonoid yield
It is respectively that 20%, 30%, 40%, 50%, 60%, 70%, 80% ethanol carries out the+microwave method that flows back with mass concentration
Carry out white-backed pseudo-ginseng total flavonoid recovery rate experiment, general flavone yield is respectively 5.2%, 5.8%, 6.7%, 9.0%, 9.7%,
8.1%th, 7.3%.
(4) solid-liquid ratio influences on white-backed pseudo-ginseng total flavonoid yield
1 is set respectively:10、1:20、1:30、1:40、1:50、1:60、1:70、1:80 solid-liquid ratios carry out the+microwave method that flows back
Carry out white-backed pseudo-ginseng total flavonoid recovery rate experiment, general flavone yield is respectively 3.1%, 6.8%, 8.9%, 9.4%, 9.9%,
10.2%th, 10.1%, 9.8%.
(5) extraction time influences on white-backed pseudo-ginseng total flavonoid yield
Using 1+1 microwave of backflow as time cycle, 1,2,3,4,5,6 backflow+microwave method is carried out respectively and carries out the white back of the body three
Seven general flavone recovery rates are tested, and general flavone yield is respectively 8.1%, 9.3%, 9.9%, 10.3%, 10.1%, 10.2%, is said
It is bright when extraction time more than 4 times after, general flavone yield does not change.
(6) microwave treatment time influences on white-backed pseudo-ginseng total flavonoid recovery rate
Using 0.5min as grade, respectively set 0.5min, 1.0min, 1.5min, 2.0min, 2.5min, 3.0min,
The 3.5min microwave digestion times, 3 wheel backflows+microwave method white-backed pseudo-ginseng total flavonoid extraction is carried out, on every grade microwave digestion time
Often wheel extraction time is identical, and general flavone yield is respectively 8.3%, 8.8%, 9.4%, 9.8%, 9.9%, 9.4%, 8.6%.
The measure of 3.1 Radix et Rhizoma Gynurae divaricatae flavones
Material powder, Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder after spectrophotometry Radix et Rhizoma Gynurae divaricatae drying and crushing and
The content of general flavone, respectively 80.6mg/g, 179.0mg/g in Radix et Rhizoma Gynurae divaricatae biscuit, 87.3mg/g, as a result as can be seen that originally
Contain abundant flavones in the Radix et Rhizoma Gynurae divaricatae biscuit of invention.
The detection of 3.2 soluble dietary fibre contents
Take and take Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, Radix et Rhizoma Gynurae divaricatae biscuit sample respectively, through α-amylase, protease and Portugal
After grape sugar former times enzyme enzymolysis, digestion, with ethanol precipitation, filtering, residue is washed with ethanol and acetone, and material is weighed as total after drying
Dietary fiber (total dietary fiber, TDF) residue；Another materials directly is filtered after above-mentioned three kinds of enzymes enzymolysis, residue
With hot wash, weigh after drying, produce insoluble diedairy fiber (insoluble dietary fiber, IDF) residue,
Filtrate obtains soluble dietary fiber (soluble dietary with being weighed after 95% ethanol precipitation of 4 times of volumes, filtering, drying
Fiber, SDF) residue.After residue drying obtained as above is weighed, protein and ash content are determined respectively.It is total dietary fiber, insoluble
The residue of dietary fiber and soluble dietary fiber, which deducts protein, ash content and blank, can calculate soluble dietary in sample
The content of fiber.
As a result the content of soluble dietary fiber is 62.4% (quality) in Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, Radix et Rhizoma Gynurae divaricatae
The content of soluble dietary fiber is 40.2% (quality) in biscuit.
3.3 animal experiment
Take more than 6 week old health and the suitable rat 30 of body weight, male is divided into I, II, III group, identical environment and
Under the conditions of carry out routine feeding, wherein：Increase daily to I rat of group and feed Radix et Rhizoma Gynurae divaricatae biscuit 25g, increase to II rat of group and feed commercially available milk
Oil cake does 25g, and group III is blank control, does not increase feeding thing, continues 6 weeks, weigh body weight weekly once, and every group takes average body weight,
As a result it is as shown in table 5 below：
Table 5：Animal feeding trials result
As can be seen that passing through the nursing of 6 months, group I is on the basis of increase feeds Radix et Rhizoma Gynurae divaricatae biscuit, rat in table 5
Body weight growth rate be 16.7%, less than the 22.0% of blank control group (group III), far below the 37.4% of group II, illustrate this hair
The bright Radix et Rhizoma Gynurae divaricatae biscuit prepared has certain antiobesity action, and more foods do not influence body weight not only, also has certain subtract
Weight function.
Embodiment 2
The special biscuit of a kind of three high crowds that the present embodiment provides, by weight, component includes：
80 parts of Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder
Resistant dextrin 1
1 part of egg
15 parts of vegetable oil
1 part of skimmed milk power
0.5 part of raising agent
0.5 part of flavor enhancement
Wherein, the preparation method of Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, with embodiment 1.
The preparation method of Radix et Rhizoma Gynurae divaricatae biscuit, with embodiment 1.
Embodiment 3
The special biscuit of a kind of three high crowds that the present embodiment provides, by weight, component includes：
70 parts of Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder
Resistant dextrin 1
2 parts of egg
18 parts of vegetable oil
2 parts of skimmed milk power
1 part of raising agent
1 part of flavor enhancement
Wherein, the preparation method of Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, with embodiment 1.
The preparation method of Radix et Rhizoma Gynurae divaricatae biscuit, with embodiment 1.
Be above a kind of detailed embodiment of the invention and specific operating process, be using technical solution of the present invention before
Put and implemented, but protection scope of the present invention is not limited to the above embodiments.
Claims (7)
1. the special biscuit of a kind of three high crowd, it is characterised in that its component and its parts by weight include：
Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder 70-80 parts；
Resistant dextrin 0.5-1 parts；
Egg 1-2 parts；
Vegetable oil 15-20 parts；
Skimmed milk power 1-2 parts；
Raising agent 0.5-1 parts；
Flavor enhancement 0.5-1 parts.
A kind of 2. special biscuit of three high crowd according to claim 1, it is characterised in that：The Radix et Rhizoma Gynurae divaricatae extract
Preparation method, comprise the following steps：
(21) divaricate velvetplant leaf is sheared, is cut after natural air drying to 3mm fritters, crushed 30 mesh sieves；According to solid-liquid ratio 1:
40 ratios add 50% volume fraction ethanol, soak 120min, obtain first time Flavonoid extract；By first time Flavonoid extract and leaching
Slag is positioned in circulation reflux device, and first time refluxing extraction is carried out under the conditions of 60 DEG C, and flow back 90min；In first time, backflow carries
After taking, medicinal extract and phase analysis are positioned over power to extract 2.5min under 360W microwaves；Enter backflow next time after Microwave Extraction to carry
Take；After refluxing extraction 2 times, stop refluxing extraction and obtain medicinal extract, be filtered under diminished pressure 40min to crude drug amount 0.1g/L, obtain the white back of the body three
Seven supernatant extract solutions；
(22) polyamide is weighed according to 2 times of mass ratioes of medicinal material, polyamide 2 times, each 1h is boiled with ethanol, in wet method
Post, it is 1 to obtain resin blade diameter length ratio:8；The concentrated extract pH3.0 that regulating step (5) obtains, soaked with will be concentrated less than 4BV/h flow velocitys
Cream is added dropwise in polyamide, and light splitting light is eluted to as eluent, 3BV/h by 70% ethanol of pH3.0 mass concentrations
Flavonoid substances are not present in degree meter detection eluent, obtain Radix et Rhizoma Gynurae divaricatae precipitation extract；
(23) sodium gluconate of 1 times of Radix et Rhizoma Gynurae divaricatae precipitation quality of addition and 1-3 times of Radix et Rhizoma Gynurae divaricatae sink in Radix et Rhizoma Gynurae divaricatae precipitation
The trisodium citrate of deposit amount, with lemon acid for adjusting pH to 6.0-7.0,2-8h is incubated at 80-100 DEG C, will after separation of solid and liquid
Liquid portion concentrates 2-3 times, and the ethanol for adding 1-2 times of volume carries out alcohol precipitation, through separation of solid and liquid, retains solid portion, obtains white
Carry on the back pseudo-ginseng precipitation extract；
(24) the Radix et Rhizoma Gynurae divaricatae supernatant extract solution of step (22) and the Radix et Rhizoma Gynurae divaricatae precipitation extract of step (23) are mixed into
Homogenate, through vacuum freeze drying, obtain Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder.
3. the special biscuit of a kind of three high crowd according to claim 1, it is characterised in that in the step (21), adopt
Collect the Radix et Rhizoma Gynurae divaricatae blade in 8-10 months as raw material.
4. the special biscuit of a kind of three high crowd according to claim 1, it is characterised in that the vegetable oil is selected from palm
One or more mixtures in oil, corn oil, sunflower oil.
A kind of 5. special biscuit of three high crowd according to claim 1, it is characterised in that the flavor enhancement be selected from salt,
One or more mixtures in essence, ground coffee.
6. the special biscuit of a kind of three high crowd according to claim 2, it is characterised in that the divaricate velvetplant leaf leads to
Following method is crossed to be prepared：
(11) tissue cultures：Radix et Rhizoma Gynurae divaricatae blade is taken under aseptic technique, improvement to be connected to vacuum side of blade as explant
In MS inducing cultures, induction differentiation obtains callus, and for light culture after 8-10 days, it is 1000-1500Lx to be transferred to intensity of illumination
In culturing room, humidity 80-85% is kept, is cultivated 8-10 days；Squamous subculture 2-3 transfers in differential medium after generation, wherein, point
Change methyl α-naphthyl acetate, the 1/2MS culture mediums of 2-5% mass fraction sucrose that culture medium is 0.2-0.4mg/L；1500-2000Lx illumination
Under intensity, cultivate 20-30 days, obtain test tube seedling；
(12) water planting：Test tube seedling is inoculated in water planting liquid, wherein, the MnSO added with 40-45mg/L4, 6.0-7.0mg/L
Zn SO4；Under natural light, cultivate 8-10 days, obtain Radix et Rhizoma Gynurae divaricatae seedling；
(13) greenhouse gardening：According to turf：Vermiculite：Fine sand is by volume (3-5):(0.5-1.5):(0.5-1.5) prepares mixing
Culture medium；By Radix et Rhizoma Gynurae divaricatae seedling cultivation in mixed culture medium, under natural light, after cultivating 5-8 days, according to 1500-2000kg/
Mu applies decomposed manure；Divaricate velvetplant leaf is harvested after 4-5 months；Ventilation in good time, irrigate.
A kind of 7. preparation method of the special biscuit of a kind of three high crowds as described in claim 1-6 is any, it is characterised in that
Comprise the following steps：
(I) Radix et Rhizoma Gynurae divaricatae extract freeze-drying powder, resistant dextrin, egg, vegetable oil, defatted milk are sequentially added according to formula rate
Powder, raising agent, flavor enhancement, often adding a kind of component will stir, and add water during stirring on demand, obtain mixing dough；
(II) semi-finished product are made according to conventional biscuit forming method in mixing dough, the Radix et Rhizoma Gynurae divaricatae biscuit is obtained after baked
Finished product.
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| CN108771713A (en) * | 2018-07-17 | 2018-11-09 | 佛山市聚成生化技术研发有限公司 | A kind of decompression fat reducing composition and preparation method thereof |
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