CN106941960A - A kind of cultivation matrix of roxburgh anoectochilus terminal bud - Google Patents
A kind of cultivation matrix of roxburgh anoectochilus terminal bud Download PDFInfo
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- CN106941960A CN106941960A CN201710194998.7A CN201710194998A CN106941960A CN 106941960 A CN106941960 A CN 106941960A CN 201710194998 A CN201710194998 A CN 201710194998A CN 106941960 A CN106941960 A CN 106941960A
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- 230000004054 inflammatory process Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000002015 leaf growth Effects 0.000 description 1
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- LPMXVESGRSUGHW-HBYQJFLCSA-N ouabain Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1C[C@@]2(O)CC[C@H]3[C@@]4(O)CC[C@H](C=5COC(=O)C=5)[C@@]4(C)C[C@@H](O)[C@@H]3[C@@]2(CO)[C@H](O)C1 LPMXVESGRSUGHW-HBYQJFLCSA-N 0.000 description 1
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- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
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- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention proposes a kind of cultivation matrix of roxburgh anoectochilus terminal bud, it is characterised in that：The cultivation matrix is prepared by the component of following weight proportion after mixing thoroughly：45 55 parts of planting soil, 15 25 parts of peanut shell, 15 25 parts of turf, 5 15 parts of sand and the water to moisten said components, the planting soil take from the place for once growing roxburgh anoectochilus terminal bud.The invention provides a kind of composition it is simple, prepare it is convenient, imitating wild planting, the roxburgh anoectochilus terminal bud cultivation matrix that disease occurs, improves roxburgh anoectochilus terminal bud seedling replanting survival rate can be reduced simultaneously, the roxburgh anoectochilus terminal bud survival rate of cultivation matrix plantation is high, imitate wild effect good, the roxburgh anoectochilus terminal bud nutritive value planted out and nutritional ingredient are close to wild gold lotus.
Description
Technical field
The invention belongs to medicinal plant artificial cultivation technique, more particularly to a kind of cultivation matrix of roxburgh anoectochilus terminal bud.
Background technology
Roxburgh anoectochilus terminal bud (Anoectochilus roburghii (Wall.) Lindl) alias Shorthairy Antenoron, rough melic herb, be
Orchid family opens lip plant flowers aspidistra and belongs to perennial Valuable Herbal Medicine, and it is wider in use scope among the people, have " king of medicine ", " gold grass ",
The laudatory titles such as " god's medicine ", " black ginseng ".《Compendium of Materia Medica》、《Chinese book on Chinese herbal medicine》、《Bencao Shiyi》、《Dictionary of medicinal plant》、《Fujian medicine
Will》、《China's economic flora》、《Zhejiang south book on Chinese herbal medicine is newly organized》Etc. the record that famous ancient books and records have roxburgh anoectochilus terminal bud.Roxburgh anoectochilus terminal bud is rich in a variety of
Brass, amino acid, taurine, sterol, polysaccharide, cardiac glycoside, trace element etc..Roxburgh anoectochilus terminal bud be distributed mainly on China's southeastern coast and
South east asia, happiness is shady and cool, moist, and growth temperature is at 20-25 DEG C, and illumination is about the 1/3 of normal illumination.Find after measured, gold
Amino acid composition in line lotus, composition, the content of content and activity of fighting against senium trace element be above domestic and wild American Ginseng and
Wild ginseng, the centuries is used as conventional herbal medicine among the people.
Roxburgh anoectochilus terminal bud main component：Containing sugar（Polysaccharide 13.326%, oligosaccharide 11.243%, reduced sugar 9.73%）, taurine, Huang
Ketone, Cardiac glycosides, esters, alkaloid, steroidal, several amino acids, trace element and inorganic elements etc..Wherein taurine, flavones,
Polysaccharide constituents have nutrition, anti-aging, improve blood circulation, can reduce cholesterol, improve cardiovascular and cerebrovascular disease, regulation people
The effect of body immunity of organism.Due to containing carbohydrate content in composition（Polysaccharide 13.326%, oligosaccharide 11.243%, reduced sugar 9.73%）、
The compositions such as taurine, flavones, Cardiac glycosides, esters, alkaloid, steroidal, thus roxburgh anoectochilus terminal bud have control spitting of blood, bronchitis, kidney
The function of the solidifying difficult illness such as inflammation, bladder fire, diabetes, blood urine, rheumatic arthritis, also there is certain effect to anticancer.
The continuous improvement of people's living standard and health care consciousness constantly strengthen, and are constantly sent out with the nutritional ingredient of roxburgh anoectochilus terminal bud
Existing, market demand just rises in the same scale, state that roxburgh anoectochilus terminal bud is in that supply falls short of demand.And because the market roxburgh anoectochilus terminal bud source of goods is short
Lack, country advocates artificial culture planting industry and exploitation roxburgh anoectochilus terminal bud energetically.Because roxburgh anoectochilus terminal bud is higher to growth environmental requirement, added
Degree harvesting, wild gold lotus is in the trend of increasingly depleted, so the technology urgent need of artificial cultivation roxburgh anoectochilus terminal bud is perfect.And in Planting
In training, roxburgh anoectochilus terminal bud is very strict to plantation environment, sanitary condition requirement, and training method relatively conventional at present is to utilize culture
Bottle carries out the mode of tissue culture, then proceedes to continue to cultivate in soilless culture medium, because roxburgh anoectochilus terminal bud is long in blake bottle cultivation technique
Phase in sterile closed Growth In Space, in addition tissue culture matrix during adapted added with a certain amount of hormone cause containing carbohydrate content,
Taurine, Cardiac glycosides, esters, alkaloid, steroidal, several amino acids, trace element and inorganic elements etc. decline to a great extent, so as to
Cause pharmacological to decline, but also there is samping off, larger with roxburgh anoectochilus terminal bud field grown environmental difference, survival rate of plant is relatively low,
Condition is poor, high cost, it is impossible to realize industrialization production.
Artificial cultivation roxburgh anoectochilus terminal bud is mainly divided to blake bottle to grow seedlings and transplant two links, and the area having at present can be adopted
Implanting and cultivating technology is carried out with the mode of compost, and the roxburgh anoectochilus terminal bud of the southern band in Zhejiang can not be cultivated using compost at present, only
Soilless culture can be carried out by the way of blake bottle tissue culture.Artificial cultivation needs seedling taking out cleaning from blake bottle when transplanting
And point cultivation is into cultivation matrix.Because roxburgh anoectochilus terminal bud is typically grown in the hill-side valley wall in remote mountains deep and secluded valley, permeable and water-retaining property is good
Hillside or stone gap are tilted, sparse mountain grass is other, under Secondary forest is cloudy, if so to reach that wild gold lotus is had when transplanting
Just need to imitate wild gold lotus growing environment during the similar pharmacological action having, roxburgh anoectochilus terminal bud is harsh to growth environmental requirement, and shows
Some roxburgh anoectochilus terminal bud seedling cultivation matrix multicomponents are complicated, and water conservation gas permeability is not good, easily causes multiple in roxburgh anoectochilus terminal bud growth course
The diseases such as samping off, soft rot, influence the transplanting survival rate of roxburgh anoectochilus terminal bud seedling.
The content of the invention
Based on above mentioned problem, present invention aims at providing, a kind of composition is simple, prepare convenient, imitating wild planting, simultaneously
The roxburgh anoectochilus terminal bud cultivation matrix that disease occurs, improves roxburgh anoectochilus terminal bud seedling replanting survival rate, the gold thread of cultivation matrix plantation can be reduced
Lotus survival rate is high, imitates that wild effect is good, and the roxburgh anoectochilus terminal bud nutritive value planted out and nutritional ingredient are close to wild gold lotus.
For problem above, there is provided following technical scheme：A kind of cultivation matrix of roxburgh anoectochilus terminal bud, it is characterised in that：It is described
Cultivation matrix is prepared by the component of following weight proportion after mixing thoroughly：45-55 parts of planting soil, 15-25 parts of peanut shell, turf 15-
25 parts, 5-15 parts of sand and the water to moisten said components, the planting soil are dug from the place for once growing roxburgh anoectochilus terminal bud
Take.
The present invention is further arranged to, and the cultivation matrix is prepared by the component of following weight proportion after mixing thoroughly：Plantation
Native 48-52 parts, 18-22 parts of peanut shell, 18-22 parts of turf, 8-12 parts of sand and the water to moisten said components.
The present invention is further arranged to, and the cultivation matrix is prepared by the component of following weight proportion after mixing thoroughly：Plantation
50 parts of soil, 20 parts of peanut shell, 20 parts of turf, 10 parts of sand and the water to moisten said components.
Beneficial effects of the present invention：The invention provides a kind of cultivation matrix of roxburgh anoectochilus terminal bud, by using new formula, and
Composition is simple, and it is convenient that material is obtained and prepared, and simulates roxburgh anoectochilus terminal bud field grown environment, under daily humiture, light conditions not
Only survival rate is high, grow fine, and is conducive to being difficult introduced disease in roxburgh anoectochilus terminal bud root development, roxburgh anoectochilus terminal bud growth course, is conducive to
Roxburgh anoectochilus terminal bud survival rate of seedling is improved, and turns out the roxburgh anoectochilus terminal bud come and imitates that wild effect is good, the nutritional ingredient such as polysaccharide and brass
Content content close in wild gold lotus, roxburgh anoectochilus terminal bud nutritive value than being cultivated in traditional planting technology is higher, survival rate more
Height, solves in traditional production technique the pharmacological of roxburgh anoectochilus terminal bud, premunition and yields poorly, high cost can not realize industrialization production
The problems such as, to promoting efficient agriculture development with extremely long-pending effect.
Embodiment
With reference to embodiment, the embodiment to the present invention is described in further detail.Following examples are used for
Illustrate the present invention, but be not limited to the scope of the present invention.
Embodiment：
The present embodiment studies it to roxburgh anoectochilus terminal bud growth and the influence of general flavone content using different cultivation matrixes
1st, 5 processing cultivation matrixes of experimental design：
Processing is 1.：Planting soil：Peanut shell：Turf：Sand weight compares 5：2：2：1；
Processing is 2.：Planting soil；
Processing is 3.:Peanut shell：Turf：Sand weight compares 2：2：1；
Processing is 4.：Planting soil：Peanut shell：Turf：Perlite weight compares 2.5：2：2：1.
Processing is 5.：Common soil：Peanut shell：Turf：Sand weight compares 5：2：2：1.
Planting soil in above-mentioned 5 processing cultivation matrix takes from the place for once growing roxburgh anoectochilus terminal bud, common native from field
Take in ground.The roxburgh anoectochilus terminal bud seedling that tissue culture goes out is transplanted in aforementioned four processing culture matrix respectively, it is random using single-factor
Block design, 3 repetitions, totally 15 cells, each 144 plants of cell is then equal under outdoor normal temperature and normal illumination
Under the conditions of cultivate, it is watered in right amount in incubation.
2nd, data acquisition and statistical method
Survival rate:30 days after transplanting, 120 days, each cell, which randomly selects 72 plants of roxburgh anoectochilus terminal bud and calculates its survival rate, (survives strain number
Multiply very than all strain numbers).
Plant height, the number of blade, fresh weight statistics:Transplanting the same day claims roxburgh anoectochilus terminal bud mean fresh, 30 days after transplanting, 120 days, each
Cell randomly selects 10 plants of roxburgh anoectochilus terminal bud, calculates its average plant height, average leaf number, mean fresh, average general flavone content, individual plant
Increment refers to that 30 days roxburgh anoectochilus terminal bud single-strain fresh weights subtract 120 days roxburgh anoectochilus terminal bud individual plants after transplanting same day single-strain fresh weight, transplanting after transplanting
Fresh weight subtracts the value of 30 days after transplanting, and variance analysis is carried out using Excel softwares.
3rd, the effective constituent determination method of roxburgh anoectochilus terminal bud
The flavones that roxburgh anoectochilus terminal bud is included content before inflorescence can reach content after highest, inflorescence and gradually decrease, the gold thread of growth 120 days
Lotus is closer to the flavones in inflorescence phase, therefore measurement roxburgh anoectochilus terminal bud using the roxburgh anoectochilus terminal bud for transplanting cultivation 120 days.Specific method：Gold thread
Lotus seedling is cultivated 120 days, and the roxburgh anoectochilus terminal bud complete stool under different disposal cultivation matrix is harvested respectively, cleans up, is placed in 60 DEG C of baking ovens
Installed after middle baking 2h, natural cooling with hermetic bag, deliver to the measure that laboratory carries out general flavone.
The assay method of flavones：Using flavone compound and Al3+Red complex is generated, extinction is determined at 510nm
Degree, and then the total amount of flavone compound is determined, standard curve is drawn by standard substance of control substance of Rutin.
4th, test results and analysis
（1）Influence of the different disposal cultivation matrix to roxburgh anoectochilus terminal bud transplanting survival rate
Table 1：Different disposal cultivation matrix is examined or check to roxburgh anoectochilus terminal bud survival rate
| Handle cultivation matrix | Transplant 30 days survival rates | Transplant 120 days survival rates |
| Processing is 1. | 100% | 100% |
| Processing is 2. | 97.7% | 97.7% |
| Processing is 3. | 99.5% | 99.5% |
| Processing is 4. | 100% | 100% |
| Processing is 5. | 95% | 91% |
As known from Table 1, influence of the different cultivation matrixes to roxburgh anoectochilus terminal bud transplanting survival rate is different, and processing is 1., 4. roxburgh anoectochilus terminal bud is moved for processing
Survival rate highest after planting 30 days, 120 days, survival rate is 100%, and 3. 2. ＞ is handled for processing, and survival rate is taken second place, and handles 5. roxburgh anoectochilus terminal bud
Survival rate is minimum after transplanting 30 days, 120 days, and survival rate be that survival rate is 91% after 95%, 120 days after transplanting 30 days, illustrates to plant
Soil is more beneficial for the survival rate of roxburgh anoectochilus terminal bud.
（2）Influence of the different disposal cultivation matrix to roxburgh anoectochilus terminal bud plant height
Table 2：Different disposal cultivation matrix is examined or check to roxburgh anoectochilus terminal bud plant height
| Handle cultivation matrix | Transplant 30 days plant heights（cm） | Transplant 120 days plant heights (cm) |
| Processing is 1. | 8.9 | 9.06 |
| Processing is 2. | 6.91 | 7.13 |
| Processing is 3. | 8.7 | 8.94 |
| Processing is 4. | 6.28 | 6.38 |
| Processing is 5. | 6.43 | 6.51 |
Influence of the different disposal cultivation matrix to roxburgh anoectochilus terminal bud plant height is different as known from Table 2, from roxburgh anoectochilus terminal bud transplant 30 days after plant height put down
From the point of view of average, can draw processing 1. ＞ processing 3. ＞ processing 2. ＞ processing 5. ＞ processing 4., its average value is followed successively by:8.9cm、
1. 8.7cm, 6.91cm, 6.43cm, 6.28cm, from the point of view of plant height average value after roxburgh anoectochilus terminal bud is transplanted 120 days, can draw processing at ＞
Reason 3. ＞ processing 2. ＞ processing 5. ＞ processing 4., its average value is followed successively by:9.06cm、8.94cm、7.13cm、6.51cm、
6.38cm, from 30 days to 120 days, illustrates that planting soil is more beneficial for the height growth of roxburgh anoectochilus terminal bud, and handle 1. to roxburgh anoectochilus terminal bud plant height
Effect is most obvious.
（3）Influence of the different cultivation matrixes to the imitative wild gold lotus number of blade
Table 3：:Different disposal cultivation matrix is to imitative wild gold lotus number of blade examination
| Handle cultivation matrix | Transplant 30 days numbers of blade (piece) | Transplant 120 days numbers of blade（Piece） |
| Processing is 1. | 4.8 | 4.8 |
| Processing is 2. | 4.8 | 4.5 |
| Processing is 3. | 4.93 | 4.2 |
| Processing is 4. | 4.73 | 3.87 |
| Processing is 5. | 4.76 | 4.03 |
As known from Table 3, influence of the different cultivation matrixes to the roxburgh anoectochilus terminal bud number of blade is different, transplants 30 days rear blade numbers from roxburgh anoectochilus terminal bud and puts down
From the point of view of average, can draw processing 3. ＞ processing 1.=processing 2. ＞ processing 5. ＞ processing 4., its average value is followed successively by:4.93,
4.8,4.8,4.76,4.73, from the point of view of roxburgh anoectochilus terminal bud transplants 120 days rear blade number average value, processing 1. ＞ can be drawn
Processing 2. ＞ processing 3. ＞ processing 5. ＞ processing 4., its average value is followed successively by:4.8,4.5,4.2,4.03,3.87
Piece, from 30 days to 120 days, 1. explanation processing can more preferably promote the leaf growth of roxburgh anoectochilus terminal bud.
（4）Different disposal imitates the influence of wild gold lotus individual plant increment
Table 4：Different disposal is to imitative wild gold lotus individual plant increment examination
| Handle cultivation matrix | Transplant increment during contrast plantation in 30 days（cm） | Transplant contrast in 120 days and transplant the increment (cm) of 30 days |
| Processing is 1. | 0.29 | 0.59 |
| Processing is 2. | 0.24 | 0.30 |
| Processing is 3. | 0.25 | 0.29 |
| Processing is 4. | 0.28 | 0.23 |
| Processing is 5. | 0.21 | 0.19 |
As known from Table 4, influence of the different cultivation matrixes to roxburgh anoectochilus terminal bud individual plant increment is different, processing 1. roxburgh anoectochilus terminal bud transplant 30 days,
The individual plant increment highest of roxburgh anoectochilus terminal bud after 120 days, 5. roxburgh anoectochilus terminal bud transplanting 30 days, 120 days individual plant increments are minimum for processing.
From the point of view of single-strain fresh weight increment average value after roxburgh anoectochilus terminal bud is transplanted 30 days, it can show that 1. ＞ handles 4. ＞ processing for processing
3. 2. 5. ＞ processing, after transplanting 120 days from the point of view of single-strain fresh weight increment average value, can show that 1. ＞ handles 2. ＞ for processing for ＞ processing
3. 5. ＞ handles 4. ＞ processing for processing, from 30 days to 120 days, it is seen that 1. processing can more preferably improve the fresh weight of roxburgh anoectochilus terminal bud, promotes to plant
Strain growth.
（5）Different disposal imitates the influence of wild gold lotus general flavone
Table 5：Measure of the different disposal to imitative wild gold lotus general flavone
| Handle cultivation matrix | General flavone content (mg/g) after transplanting 120 days |
| Processing is 1. | 5.875 |
| Processing is 2. | 5.429 |
| Processing is 3. | 4.939 |
| Processing is 4. | 5.661 |
| Processing is 5. | 4.778 |
As known from Table 5, its general flavone content is measured to be followed successively by：Processing 1. ＞ processing 4. ＞ processing 2. ＞ processing 3. ＞ processing 5.,
It can be seen that planting soil is conducive to improving the flavones content of pseudo-wild cultivating roxburgh anoectochilus terminal bud, and handle 1. raising roxburgh anoectochilus terminal bud flavone component content
It is the most obvious.
5th, conclusion
Shown by above-mentioned result of the test, different cultivation matrixes are to roxburgh anoectochilus terminal bud transplanting survival rate, plant height, blade, fresh weight, general flavone etc.
Aspect has different influences, and experimental data shows that the roxburgh anoectochilus terminal bud cultivated by planting soil grows more preferably, and common soil can not
The cultivating and growing demand of roxburgh anoectochilus terminal bud is met, and is shown by the data result cultivated 30 days to the data result of cultivation 120 days, is located
Reason 1. under the conditions of the roxburgh anoectochilus terminal bud survival rate cultivated, plant height, blade, fresh weight, general flavone be optimal, the processing cultivation matrix is not
Only promote growing for roxburgh anoectochilus terminal bud plant, not only survival rate is high under daily humiture, light conditions, grow fine, and approaches
Introduced disease is difficult in the natural growing environment of roxburgh anoectochilus terminal bud, roxburgh anoectochilus terminal bud growth course, is conducive to improving roxburgh anoectochilus terminal bud survival rate of seedling,
And turn out the roxburgh anoectochilus terminal bud come and imitate that wild effect is good, the content of the nutritional ingredient such as polysaccharide and brass contains close in wild gold lotus
Amount, while planting soil：Peanut shell：Turf：Sand weight compares 5：2：2：1 is optimal.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, on the premise of the technology of the present invention principle is not departed from, some improvement and modification can also be made, above-mentioned hypothesis these
Improve and modification also should be regarded as protection scope of the present invention.
Claims (3)
1. a kind of cultivation matrix of roxburgh anoectochilus terminal bud, it is characterised in that：The cultivation matrix is by the component of following weight proportion through mixing thoroughly
After prepare：45-55 parts of planting soil, 15-25 parts of peanut shell, 15-25 parts of turf, 5-15 parts of sand and to moisten said components
Water, the planting soil takes from the place for once growing roxburgh anoectochilus terminal bud.
2. a kind of cultivation matrix of roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：The cultivation matrix is by following heavy
The component of amount proportioning is prepared after mixing thoroughly：48-52 parts of planting soil, 18-22 parts of peanut shell, 18-22 parts of turf, 8-12 parts of sand with
And to moisten the water of said components.
3. a kind of cultivation matrix of roxburgh anoectochilus terminal bud according to claim 1, it is characterised in that：The cultivation matrix is by following heavy
The component of amount proportioning is prepared after mixing thoroughly：50 parts of planting soil, 20 parts of peanut shell, 20 parts of turf, 10 parts of sand and to moisten
The water of said components.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107400009A (en) * | 2017-07-27 | 2017-11-28 | 江苏九谷生态农业有限公司 | A kind of pot flowers Nutrition Soil and preparation method thereof |
| CN108668836A (en) * | 2018-04-28 | 2018-10-19 | 福建省大山金线莲科技开发有限公司 | A kind of cultivation matrix and its cultural method of the nursery of roxburgh anoectochilus terminal bud high viability |
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