CN105613178A

CN105613178A - Method for cultivating acanthopanax sessiliflorus trees and acanthopanax tea with weight reducing function

Info

Publication number: CN105613178A
Application number: CN201610023899.8A
Authority: CN
Inventors: 隋丽梅
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-01-15
Filing date: 2016-01-15
Publication date: 2016-06-01

Abstract

The invention relates to a method for cultivating acanthopanax sessiliflorus trees and acanthopanax tea with a weight reducing function, and belongs to the field of health care tea. The method for cultivating the acanthopanax sessiliflorus trees is characterized in that (1) residues obtained after medical and edible dual purpose plants of oolong, poria cocos, lotus leaves, hawthorns, chrysanthemum and the like are extracted and are applied as a root fertilizer for the acanthopanax sessiliflorus trees; (2) from the second day of picking tender leaves of acanthopanax sessiliflorus, liquor prepared from extracts of the oolong, the poria cocos, the lotus leaves, the hawthorns, the chrysanthemum and the like and ginseng distilled water is sprayed to leaf faces every day; (3) the acanthopanax tea obtained by stir-frying the tender leaves of the acanthopanax sessiliflorus is prepared according to a tea production process by manual stir-frying. After being taken by a fat person, the acanthopanax tea disclosed by the invention also has effects of balancing blood pressure, relieving fatigue, deferring senility, reducing an occurrence rate of headache and enhancing the memory ability when having obvious lipid decreasing and weight reducing effects. The acanthopanax tea is suitable for people of all ages and both sexes. The tea has no any toxic or side effects and has no untoward effects when being taken.

Description

A kind of method cultivating Cortex Acanthopancis tree and there is the WUJIA CHA of weight losing function

Technical field

The present invention relates to a kind of method cultivating Cortex Acanthopancis tree and there is the WUJIA CHA of weight losing function, belonging to functional alternative tea field.

Background technology

Obesity is a kind of common chronic disease, and especially in some economically developed countries, obesity patient increases severely. France's " science and life " periodical was once reported, whole world obesity patient is more than 4 hundred million at present, and is still being on the increase, especially serious with America and Europe. In our country, along with the improvement gradually of eating condition, obesity patient is also increasing gradually. In the Liverpool, UK An Shu hospital pharmacology professor GerethWillams nearest 10 years world's incidence of obesity ranking list statistical graphs announced, China is listed in the 10th impressively. The nutritional survey report of China also indicates that, over 20 years, 4 overweight rates of regional Adult Groups such as Beijing, Shanghai have exceeded 30%. There is very big regional difference in China in overweight and obesity rates, the north is higher than south; Developed area exceedes backward areas; Women exceedes male. It addition, the simple obesity sickness rate of child is also improving constantly, according to investigations, the report that Beijing childhood obesity incidence rate is front than 10 years has had growth at double. One research shows, 7 years old Obese children of 40% and the Adolescent Obesity teenager of 70% still suffer from obesity after growing up. Therefore, children obesity must treated as soon as possible.

Obesity can cause metabolism and endocrine regulation, and can increase the initiation potential of the diseases such as type ii diabetes, atherosclerosis, hyperlipidemia, hypertension, coronary heart disease. Thus obesity has become as current comparatively general social medicine problem.

Up to now, the method for relatively conventional prevention and treatment obesity has pharmacotherapy, dietetic therapy, exercise therapy and the behavior therapy four kinds. The medicine with fat-reducing effect is mainly appetite inhibitor, accelerates the hormone of metabolism, affects the medicine etc. digested and assimilated with lipid mobilization. Appetite suppressant is regulated by the effect of catecholamine and 5-hydroxy tryptamine mediator to ingest and diet maincenter mostly, reduces appetite, so that weight loss. This kind of drug main to have amfetamine and derivant Fenfluramine etc. thereof. The hormone and the medicine that accelerate metabolism mostly make metabolic rate rise by the raw heat of increase, thus reaching fat-reducing purpose. They mainly have thyroxin, growth hormone and intend beta-adrenergic medicine etc. The medicine that impact is digested and assimilated, mainly by the emptying time extending stomach, increases satiety, and the factors such as energy and nutraceutical absorption that reduce make weight loss. These medicines include edible fiber, Olestra etc. And mobilizing fat-splitting medicine is by stimulating beta receptor or suppressing a₂Receptor promotes fat oxidation heat production to reach fat-reducing purpose. Such as norepinephrine, Yohimbine etc.

Although these medicines all have antiobesity action, but mostly have certain side effect, it is easy to cause nausea, the untoward reaction such as abdominal distention, diarrhoea and functional lesion, after drug withdrawal, also easily cause bounce-back. And while Drug therapy, cooperation low caloric diet of generally still needing is to increase fat-reducing effect. It is true that be not only pharmacotherapy, even exercise therapy and the behavior therapy also need in conjunction with hyposite. As can be seen here, dietetic therapy is method of weight-reducing the most basic, safest. Therefore screening has the beverage of the pure natural of antiobesity action, it has also become an important topic in fat-reducing research process. People to better effects if, have no side effect and the beverage with weight losing function, there is substantial amounts of demand.

It should be give full play to the effect that body health is favourable that human body desirably regulates function of obesity, is reduced as far as or eliminates body adverse influence. In the middle of long-term research and practice, it is found that nature also exists various material that can regulate body function of obesity, these materials transferring the regulative mechanism of body, improve body resistance against diseases, maintain own physiological balance etc. in played positive effect.

China has long tea culture, and a lot of people are used as having tea as the enjoyment of one way of life. And the health tea much with specific function occurred on market, it is formulated by Folium Camelliae sinensis and some health foods, in order to make its effective ingredient better be dissolved in water, these health teas are generally all ground into little granule, therefore this health tea is contained in little paper bag or non-woven bag more, when having tea, teabag is directly placed in cup to soak and drinks, but the quality of these Related products all also exists the defect that situation is different. Such as: prior art has been disclosed a kind of health tea with weight losing function, the Radix Et Caulis Acanthopanacis Senticosi green tea announced in number of patent application 02109604.X, it is that raw material is made by Wilsonii raw pulp extract and green tea, this tea probably due to granule is little and leak-off pocket, to stand a little while when drinking, and do not have the visual effect that conventional tea is immersed in cup, and therefore the people that samples tea being liked for those, they do not like and drink teabag.

Summary of the invention

For the defect in above-mentioned field, the present invention provides a kind of technique cultivating Cortex Acanthopancis tree and plucks the WUJIA CHA with weight losing function that this tree tender leaf prepares, in parch process, other any raw material and adjuvant need not be added, but by optimizing the cultivating technique of Cortex Acanthopancis tree, make overweight people drink this tea time and can substantially observe the effect of lowering blood-fat and reducing weight, simultaneously this tea-drinking with very convenient, do not have unqualified, and taste sweetness is moderate.

The method cultivating Cortex Acanthopancis tree, it is characterised in that: (1) uses as root fertilizer with the residue after the extractions such as medicine food dual purpose plant oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi; (2) from the first two sky that Cortex Acanthopancis tender leaf is plucked, every day, described nutritional solution was formulated by the extracts such as oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi and ginseng distilled water at foliage spray nutritional solution; (3) WUJIA CHA become by the preparation of its Cortex Acanthopancis tree tender leaf, is process for tea manufacture traditionally, forms through artificial parch completely, rather than dry in the sun forms as dry in the sun leaves.

Residue applied amount after described plant extract is 50-60 kg/acre.

Described root fertilising be by plant extract after residue embedment Cortex Acanthopancis tree root.

Described root fertilizing time is that freeze-up soil is after spring breaks a seal.

The extracts such as described oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi are their distilled water extraction thing, and the time of extraction and number of times be: 60 minutes extraction times first, 40 minutes second extraction time.

The weight ratio of the extracts such as described oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi and ginseng distilled water is 1: 10-12.

The fountain height of described nutritional solution is 50-80Kg/ mu/sky, and each sprinkling has just started drip with blade face and has been as the criterion.

The spraying time of described nutritional solution is: the dew in morning carries out after being dried by the sun immediately.

Have the WUJIA CHA of weight losing function, said method the tender leaf parch of the Cortex Acanthopancis tree cultivated forms.

Cortex Acanthopancis tree uses the residue after medicine food dual purpose plant extracts and the mode that combines of foliage spray nutritional solution by special cultivating technique and root, after the soil Kaifeng that annual spring is freeze-up, root uses the residue after medicine food dual purpose plant extracts, by the weight portion of 60 kilograms every mu, dig the residue after appropriate medicine food dual purpose plant is extracted by a pitting by strain at Cortex Acanthopancis usage tree root to apply, then with grave lid, during early spring, namely executed object can decompose gradually and absorbed by plant root in soil; Pluck the first two sky at Cortex Acanthopancis tender leaf and carry out foliage spray nutritional solution, the weight ratio of the extracts such as oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi Yu ginseng distilled water is configured to nutritional solution with 1: 10-12, to spray blade face amount for 80Kg/ mu/sky, each sprinkling has just started drip and has been as the criterion with blade face, is entered in stamen leaf and participate in the body-internal-circulation of Cortex Acanthopancis plant by blade face pore.

Cortex Acanthopancis tender leaf is the leaf on the tender stem that the early spring, Cortex Acanthopancis tree newly sent, and locals is also referred to as bur rod leaf. Cortex Acanthopancis tender leaf picks by described position, according to a conventional method parch Folium Camelliae sinensis, and the prepared present invention has the WUJIA CHA of weight losing function.

Teaching and the checking such as professor Li Famei of Shenyang Pharmaceutical University through the Ma little Jun of China Medical Sciences Academy Medical Plants Institute, Cortex Acanthopancis is Araliaceae machaka, equal with Radix Et Caulis Acanthopanacis Senticosi belongs to together. Thorn on Cortex Acanthopancis stem, branch is little, is bordering on stingless, is distributed on the south Changbai Mountain, Jilin Province and the Changbai Mountain Yu Mai of east, Liaoning Province, Deng Ge mountain area, numerous mountains mountain range. In history in Hebei, Pekinese's Yanshan mountain range also once had distribution. Cortex Acanthopancis branch and leaf are luxuriant, and flowers and fruits are unusual, except being except one views and admires tree, or a kind of wild vegetable and important medicinal plants of keeping healthy. Cortex Acanthopancis is containing Alkaloid, heart tonifying work glycoside and 0.2% volatile oil and nicotinic acid, containing 0.23% cardiotonic glycoside, 0.1% volatile oil and a small amount of Saponin. Cortex Acanthopanacis Sessiliflori pharmacological action is multifarious, has strengthening by means of tonics, reduces blood fat, adjusts the effects such as blood pressure, nerve, blood glucose and cholesterol.

Cortex Acanthopancis is containing Cortex Acanthopancis polysaccharide and Cortex Acanthopancis glycosides A (eleutherosideA-cupreol glucoside), Cortex Acanthopancis B (syringoside syringin), Cortex Acanthopancis glycosides B1 (6,8-dimethoxy coumarin-7-glucoside), Cortex Acanthopancis glycosides L (galactite glycosides), Cortex Acanthopancis glycosides D and E (being the glucoside of the syringaresinol of two kinds of various configurations) etc. We are in the impact on normal mouse and immunosuppressed mice immune organ cell number and stereological parameter of the experimental study Cortex Acanthopancis polysaccharide. It is shown that Cortex Acanthopancis polysaccharide can strengthen mouse spleen and the cell number of mesenteric lymph node, spleen white pulp cumulative volume and cortex of lymph node cumulative volume, can substantially resist the immunosuppressive action of caused by cyclophosphamide. Conclusion: Cortex Acanthopancis polysaccharide has the effect strengthening immune function of mice. This is also the Main Function that Cortex Acanthopancis polysaccharide has to human body enhancing immunity function. Research also confirms that, the AccmRNA content compared with normal mice in injury of hypothalamus adipose tissues substantially reduces, and after taking in Cortex Acanthopancis polysaccharide-4, the AccmRNA level in its fatty tissue significantly improves. Therefore, its functions of lowering blood-fat and reducing weight may be by regulating what energy i (in vivo) metabolism realized.

Oolong tea is described as " tea of weight reducing " entrance Japan the seventies, and quickly spreads in the young woman paying close attention to fat-reducing, and the research of health efficacy starts to spread out. In these more than 30 years, study through numerous research worker, disclose various effect. Include: obesity, suppress blood pressure to rise, improve medium-sized fat, reduce cholesterol value, suppress blood glucose to rise, anticancer, skin Caring, beauty treatment, improve anaphylaxis, various effects such as rhythm of making the life better, prevention decayed tooth. Fujian Institute of Traditional Chinese Medicine has carried out following experiment: with Simple Obesity for the object of observation, drinks continuously after oolong tea, carries out clinical observation for the regulatory function suppressing fat effect and phat fat metabolism. During experiment, stop all regimen being likely to have fat-reducing effect and exercise therapy, it is desirable to their normal diet within the time of 6 weeks. Experimental result: simple obesity patient 75 example, weight loss, what remarkable result occur accounts for 15%, and what effective effect occur accounts for 52%, and total effective rate reaches 67%. Oolong tea has the effect of burning fatty acid, function in intensive aspect in promotion blood, improves heat consumption.

The dried leaves of Folium Nelumbinis Folium Nelumbinis system Nymphaeceae platymiscium lotus NelumbonuciferaGaertn.. Traditional medicine thinks Folium Nelumbinis nature and flavor bitter cold have clearing away summer-heat and eliminating dampness, and hair growth promoting is positive clearly, clearing away heart-fire reduce phlegm and internal heat hemostasis diuretic activity. " Folium Nelumbinis takes it, makes us thin bad ", " hair growth promoting vigour, benefit helps taste, and arresting seminal emission is turbid, scattered silt blood, detumescence pain, sends out variola " is recorded according to Compendium of Material Medica. Auxiliary blood fat reducing and weight losing function, observing the impact on serum total cholesterol (TC) and triglyceride (TG) of the Folium Nelumbinis water extract with acute hyperlipemia mice for block mold, treated in vitro observes medicine has suppression trend to hepatocyte synthetic cholesterol. With Folium Nelumbinis alcohol extract, hyperlipemia in mice being carried out pharmacological evaluation, result proves that Folium Nelumbinis alcohol extract can substantially reduce mice TC content, there was no significant difference with positive controls (inositol nicotinate sheet). With Folium Nelumbinis biology total alkali for index, investigate the impact on fat hyperlipidemia rats blood fat and body weight of such material. Result shows, Folium Nelumbinis biology total alkali 2.14��10.7mg/kg all can reduce TC, TG and the body weight growth figure such as Lee ' s index of obese rat, compare with model group that there were significant differences (P < 0.05 or P < 0.01) and there is dose dependent, but high density lipoprotein (HDL-C) is acted on inconspicuous. Experiment proves that Folium Nelumbinis electuary can reduce TC, TG content in the tentative hyperlipemia of rabbit, and lipid precipitation in animal tissue and atherosclerosis are all had inhibitory action. By the Intervention trial to hyperlipidemia animal model, confirm that Folium Nelumbinis can slow down the increase of obese rat body weight, promote fat cell metabolism, reduce adipose cell synthetic glycerine three ester and suck fatty acid, enzyme hydrolysis and the free fatty of strengthening triglyceride discharge in blood, have certain functions of lowering blood-fat and reducing weight.

Poria is the dry sclerotia of Polyporaceae plant Poria Poriacocos (Schw.) Wolf. Sweet in the mouth, light, property is put down. GUIXIN, spleen, lung meridian. Function: eliminating dampness and diuresis, strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming. Cure mainly: dysuria, edema distension, phlegm retention cough with dyspnea, nausea and vomiting, have loose bowels, pass out semen, stranguria with turbid discharge, palpitation with fear, forgetful.

Fructus Crataegi, fruit for rosaceous plant Fructus Crataegi CrataeguspinnatifidaBge. or Crataegi cuneatae. Also Fructus Pyracanthae (" 101 choosing side ") in famous mountain. Sour in the mouth, sweet, slightly warm in nature. Return spleen, stomach, Liver Channel. Function: help digestion long-pending, scattered silt blood, expelling tenia.

Flos Chrysanthemi, this product are the dry capitulum of feverfew. Containing echinacoside, chloro acid, flavonoid and mcg vitamin B1 in spending. Pharmacological action, this product coronary artery dilating, increase coronary flow, reduce blood pressure, resist local capillary permeability. In addition with antipyretic, antibacterial etc. Modern times have for treating hypertension, arteriosclerosis, coronary heart disease etc.

People utilize fresh ginseng for raw material in Radix Ginseng producing region, when processing Radix Ginseng Rubra, having one procedure is steam ginseng, in this procedure, create substantial amounts of ginseng distilled water, often processing 100kg Radix Ginseng, the ginseng distilled water of about 150kg can be produced, these distilled water are generally all thrown away in vain, all without carrying out comprehensive utilization, we test proves that, these ginseng distilled waters contain substantial amounts of ginsenoside, can be made full use of it completely in processing of Panax ginseng process, the high temperature sterilize just naturally simultaneously completed when steaming ginseng, and utilize the extraneous natural temperature (average temperature is-5 DEG C to 5 DEG C) of red ginseng processing production period, this temperature is conducive to these ginseng distilled waters can put in container, take care of to the three of the coming year, four, the production and application phase between May.

The contrast test experiment material of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide content that we do: tested material (experimental example 2 prepare WUJIA CHA) and reference substance (gloomy source, Beijing slender acanthopanax beverage company limited sells, on August 1st, 2006 produces, gloomy source board radix Acanthopanacis senticosi tea)

Radix Et Caulis Acanthopanacis Senticosi polysaccharide extracting method in Cortex Acanthopancis adopts microwave loss mechanisms: take tested material and reference substance 500g respectively, after broken, respectively weigh 100g and put in 500ml bottle, this flask is put in MCL-3 type curved continuous box girder, successively with 250mL petroleum ether (60��90 DEG C), ether and 80% alcohol reflux, adjust power 560W, 500W, 400W, 350W, 20 minutes response time. After residue volatilizes solvent, place in MCL-3 type curved continuous box girder, continue with water reflux, extract, 20 minutes, adjust power 630W, 600W, 560W, 450W, it is evaporated to-half volume, adds 0.1% activated carbon, decolouring, filter, filtrate adds 95% ethanol makes solution containing alcohol 80%, stands overnight, filters, residue ether, dehydrated alcohol cyclic washing, obtain Cortex Acanthopancis polysaccharide. Weighing after 60 DEG C of drying, this product is pressed dry product and is calculated, containing Radix Et Caulis Acanthopanacis Senticosi polysaccharide 0.075% in tested material, containing Radix Et Caulis Acanthopanacis Senticosi polysaccharide 0.025% in tester. Thus contrast Radix Et Caulis Acanthopanacis Senticosi polysaccharide contained by the Radix Et Caulis Acanthopanacis Senticosi that contained Radix Et Caulis Acanthopanacis Senticosi polysaccharide in the Cortex Acanthopancis of visible breeding method of the present invention plantation is planted than conventional method and have more 2 times more than. Therefore the Cortex Acanthopanacis Sessiliflori obtained by the present invention has better health care than conventional radix Acanthopanacis senticosi tea.

Modern Chinese medicine is thought, the obesity cause of disease is complicated, and each disease type haves both at the same time clinically, and treatment giving consideration to both the incidental and fundamental suitable with health care, the same use of reinforcing and reducing, number method is also executed, and can obtain reasonable effect.

Modern Chinese medicine it is also believed that obesity distinguish that disease typing can be divided into five kinds: 1. Splenic asthenia and obstruction of dampness clinical manifestation be that obesity, the tired weight of limbs, fatigue and weakness, distension and fullness in the abdomen, poor appetite lack of appetite, big loose stool be thin, pale tongue, greasy and thin fur, moderate pulse or moisten carefully. This kind of is the most common clinically. Health care principle is invigorating the spleen for eliminating dampness. 2. spleen kidney both deficiency type clinical manifestation be obesity, impractical swelling, fatigue and weak, deficiency of QI with disinclination to talk, dynamic pant, dizzy fear of cold, lack of appetite poor appetite, chills and pain of the waist and kness, big loose stool be thin or Diarrhoea, impotence, pale tongue, white and thin fur, deep-thready pulse. Severe obesity patient mostly is this kind of. Health care principle is warming YANG activating QI to promote diuresis. 3. the clinical manifestation of gastric heat damp-obstruction type is obesity, likes food delicious food or rapid digestion of food and polyorexia, halitosis xerostomia, constipation, red tongue, yellowish fur, slippery and rapid pulse. This kind of mostly is the young and middle-aged overweight people that body is strong. Health care principle is clearing away heat and eliminating dampness purging FU-organs. 4. qi stagnation and blood stasis type clinical manifestation be obesity, two side of body distension, gastral cavilty feeling of fullness, irritable, dry mouth and tougue, have a dizzy spell, insomnia and dreamful sleep, menoxenia or amenorrhea, dark tongue quality have ecchymosis, wiry and frequent pulse or thin string. Obesity with the passing of time visible this kind of. Health care principle is liver-smoothing, qi-regulating, blood circulation promoting and blood stasis dispelling. 5. kidney yin deficiency type clinical manifestation is obesity, dizzy headache, dysphoria with feverish sensation in the chest palms and soles, soreness of the waist and knees, the few tongue of red tongue, thready and rapid pulse or thin string. This kind of is more rare clinically. Health care principle is enriching yin and nourishing kidney.

We are with Cortex Acanthopanacis Sessiliflori for monarch drug, lung benefiting asthenic fire cloudy, clear, replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization, nourishing the liver and kidney, replenishing vital essence to improve eyesight, and obesity, prevention fat absorption, reduction total lipid level and TG content reach effect of weight reducing simultaneously; With oolong tea, Folium Nelumbinis, Poria extract for ministerial drug, benefit helps stomach function regulating, and eliminating dampness and diuresis, help digestion long-pending, scattered silt blood, simultaneously digestion promoting, reduce blood pressure; Help again and make material with the extract of Fructus Crataegi, this two tastes wholefood seasoning plant of Flos Chrysanthemi as the assistant of this prescription, minimizing can be played and delay digesting and assimilating of nutrient, reduce blood fat, reduce the absorption to monosaccharide, fatty acid synthesis in vivo is made to decline, thus controlling the growth of body weight, so the purpose of fat-reducing can be reached. The fat-reducing effect making this prescription is better more comprehensively.

Simultaneously, we oolong tea, Folium Nelumbinis, Poria, Fructus Crataegi, this five kind of plant of Flos Chrysanthemi extract after, residue makees base fertilizer, extract does Foliage nutrient solution and sprays. Cortex Acanthopancis leaf is made to be primary raw material and the absorption carrier of nutritional solution, the problem decreasing the bad assurance of dosage size equalization of additive in tea frying process. So that our WUJIA CHA product quality safety and stability more, and be conducive to convenient production, simplify the production technology of tea frying link.

Extract used by the present invention all adopts the water solution extract of medicine food dual purpose plant, the temperature of backflow is lower than 100 DEG C, the effective ingredient in medicine is made not easily to change because of high temperature, adopt vacuum concentration when concentration simultaneously, the effective ingredient in extracting solution is made to be not affected by high temperatures, avoid some to the destruction of heat-labile effective ingredient and loss, save nutritional labeling and the fragrance of medicine and food raw materials better. The dry extract obtained after concentration need to blend into nutritional solution and could use.

Originally have the WUJIA CHA of weight losing function, said method the tender leaf parch of the Cortex Acanthopancis tree cultivated forms. By test example, its formula and WUJIA CHA production technology thereof, show that WUJIA CHA product effect of the present invention, test include function assessment test and safety toxicology test.

Present invention optimizes the breeding method of Cortex Acanthopancis tree, by the way of plant absorption nutrient as root fertilising, residue after medicine food dual purpose plant oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi etc. being extracted fills in the root of Cortex Acanthopancis plant, uses as root fertilizer; (3) WUJIA CHA become by the preparation of its Cortex Acanthopancis tree tender leaf, is process for tea manufacture traditionally, forms through artificial parch completely, rather than dry in the sun forms as dry in the sun leaves. Adopt again as plant leaf surface applied fertilizer, executes water way by us with the formulated mixed solution of the extracts such as oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi and ginseng distilled water, before plucking Cortex Acanthopancis tender leaf, reasonable time carries out foliage spray, carries out plant absorption and photosynthesis, makes the weight losing function of Cortex Acanthopancis Folium Camelliae sinensis be improved.

The WUJIA CHA of the present invention, give the suitable population such as physical labourer and brain worker, student, old man respectively to take, thus parts of body being adjusted to happy state, substantially alleviate the obesity that a variety of causes causes, thus commonly encountered diseases reduces, energetic, it is achieved the function of the fat-reducing that the present invention has.

Aqueous solution when ginseng distilled water is to steam Radix Ginseng, is often directly emit, but more or less has some ginseng effective components to be dissolved in water in the process steam Radix Ginseng, and therefore this raw material is all twice laid, becomes the perfect fertilizer cultivating Cortex Acanthopancis tree. Cortex Acanthopanacis Sessiliflori early has application as alternative tea, but its bitter in the mouth, common people are difficult to swallow, currently preferred Fructus Crataegi and this two tastes medicine food dual purpose plant of Flos Chrysanthemi are also aided with ginseng distilled water, they are except powerful to fat-reducing, utilize it sour, sweet simultaneously and the peculiar fragrance of Radix Ginseng is to regulate the taste of tea, make the WUJIA CHA of the present invention become the mass product that consumer is willing to accept.

WUJIA CHA of the present invention does not add all the other dispensings in parch process, but make function tea of the present invention by optimizing the cultivating technique of Cortex Acanthopancis tree, overweight people is made can substantially to see the effect of fat-reducing when drinking this health tea, this Folium Camelliae sinensis is very convenient when drinking simultaneously, there is no that complex tea frying formed various unqualified, taste is sour-sweet moderate simultaneously.

The present invention has the WUJIA CHA instructions of taking of weight losing function:

(1) as long as needing slimmer to drink after brewing with 80 DEG C of hot boiled water.

(2) WUJIA CHA parch of the present invention can be become as phoenix singly from the various tea types such as Radix Campylotropis Hirtella (Herba Myrsines Africanae), drink to facilitate.

(3) this product is cold and hot after brewing drinks, and hot drink energy warming the stomach, cold drink can quench one's thirst, and the effect of fat-reducing is constant.

Many suitable population are trying out generally reflect it is clearheaded after by WUJIA CHA of the present invention, and the big tripe of fat reduces, feeling relaxed, and health fatiguability is eased, and immune function improves, and major part has all broken away from medicine relevant to fat-reducing for many years.

Clinical verification, WUJIA CHA of the present invention is except having effect of obvious fat-reducing, moreover it is possible to balancing blood pressure, reduce blood fat, alleviating physical fatigue, slow down aging, reduction headache incidence rate, improve one's memory, somebody also have increase working strength effect. Drink this tea sutable for men, women, and children, and without any side effects and untoward reaction.

Detailed description of the invention

Below in conjunction with embodiment 1 and embodiment 2, WUJIA CHA of the present invention is described in further detail.

Embodiment 1

Feed proportioning: oolong tea 20kg, Folium Nelumbinis 20kg, Poria 20kg, Fructus Crataegi 20kg, Flos Chrysanthemi 20kg.

Its preparation method: the preparation method of the WUJIA CHA of the present invention according to claims and this specification is prepared.

1.1 root fertilisings

After the soil Kaifeng that annual spring is freeze-up, root uses the residue after medicine food dual purpose plant extracts, by the weight portion of 60 kilograms every mu, dig the residue after appropriate medicine food dual purpose plant is extracted by a pitting by strain at Cortex Acanthopancis usage tree root to apply, then with grave lid, during early spring, namely executed object can decompose gradually and absorbed by plant root in soil.

1.2 foliage dressing

The ratio of fountain height and Cortex Acanthopancis tender leaf: every mu of plantation Cortex Acanthopancis plant is 4200 strains, every strain tender leaf yield is 0.05kg, the fresh leaf-making quantity of every mu of Cortex Acanthopancis tender leaf is 210kg, the weight ratio of the extracts such as oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi and ginseng distilled water is configured to aqueous solution with 1:10, fountain height is 80Kg/ mu/sky, and fresh leaf tea frying is 21kg/ mu after giving money as a gift.

1.2.1 weighing medicine and food raw materials: oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, each 20Kg of Flos Chrysanthemi, the quality standard of above-mentioned raw materials, except pressing, except oolong tea, the Folium Camelliae sinensis standard that country is relevant, all the other are pressed under the Pharmacopoeia of the People's Republic of China one item of version in 2010.

1.2.2 oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi distilled water being refluxed 3 hours, concentrate after extracting twice, concentration adopts vacuum concentration. Vacuum concentrates also known as concentrating under reduced pressure, evaporates at a lower temperature, is possible not only to save mass energy; Simultaneously as material is not affected by high temperatures, it is to avoid the destruction of thermally labile component and loss, save nutritional labeling and the fragrance of raw material better. Dry at 50-60 DEG C after concentration, prepare dry extract 20Kg;

1.2.3 its dry extract is broken into 200 order powder, then according to the ratio of 1: 10 joins in ginseng distilled water, makes ginseng distilled water aqueous solution 200kg.

1.2.4 select in the season winning Cortex Acanthopancis tender leaf, in the first two sky winning tender leaf, the aqueous solution made is loaded in the aerosol apparatus that get ready, up-to-standard, specification adapts to, the technical staff that tissue is trained, to the blade face being about to the Cortex Acanthopancis tender leaf won, divide three times by the aqueous solution of every mu of 80kg and implement metering spray. Every morning carries out after the sun dries dew, (in case of the rainy day needs to fill spray in time after spray), implements three days continuously.

1.2.5 selecting the morning that weather is fine, the technical staff that tissue is trained, what carry out Cortex Acanthopancis tender leaf wins work. Win the fresh tender leaf got off, dry in air in the dry in the sun Dropbox that rustless steel or wicker etc. are made will be placed on, it is impossible to stacking in heaps, it is prevented that generate heat, go mouldy.

1.2.6 by the frying production method of traditional tea, become Folium Camelliae sinensis with technique parches such as routine complete, or cake of press or pack evacuation are packed, and are WUJIA CHA of the present invention.

Embodiment 2

Feed proportioning: oolong tea 30kg, Folium Nelumbinis 30kg, Poria 30kg, Fructus Crataegi 30kg, Flos Chrysanthemi 30kg.

2.1 root fertilisings

2.2 foliage dressing

The ratio of fountain height and Cortex Acanthopancis tender leaf: every mu of plantation Cortex Acanthopancis plant is 4200 strains, every strain tender leaf yield is 0.05kg, the fresh leaf-making quantity of every mu of Cortex Acanthopancis tender leaf is 210kg, the weight ratio of the extracts such as oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi and ginseng distilled water is configured to aqueous solution with 1: 10, fountain height is 80Kg/ mu/sky, and fresh leaf tea frying is 21kg/ mu after giving money as a gift.

2.2.1 weighing medicine and food raw materials: oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, each 30Kg of Flos Chrysanthemi, the quality standard of above-mentioned raw materials, except pressing, except oolong tea, the Folium Camelliae sinensis standard that country is relevant, all the other are pressed under the Pharmacopoeia of the People's Republic of China one item of version in 2010.

2.2.2 oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi distilled water being refluxed 3 hours, concentrate after extracting twice, concentration adopts vacuum concentration. Vacuum concentrates also known as concentrating under reduced pressure, evaporates at a lower temperature, is possible not only to save mass energy; Simultaneously as material is not affected by high temperatures, it is to avoid the destruction of thermally labile component and loss, save nutritional labeling and the fragrance of raw material better. Dry at 50-60 DEG C after concentration, prepare dry extract 30kg;

2.2.3 its dry extract is broken into 200 order powder, then according to the ratio of 1: 10 joins in ginseng distilled water, makes ginseng distilled water aqueous solution 300kg.

2.2.4 select in the season winning Cortex Acanthopancis tender leaf, in the first two sky winning tender leaf, the aqueous solution made is loaded in the aerosol apparatus that get ready, up-to-standard, specification adapts to, the technical staff that tissue is trained, to the blade face being about to the Cortex Acanthopancis tender leaf won, divide three times by the aqueous solution of every mu of 80kg and implement metering spray. Every morning carries out after the sun dries dew, (in case of the rainy day needs to fill spray in time after spray), implements three days continuously.

2.2.5 selecting the morning that weather is fine, the technical staff that tissue is trained, what carry out Cortex Acanthopancis tender leaf wins work. Win the fresh tender leaf got off, dry in air in the dry in the sun Dropbox that rustless steel or wicker etc. are made will be placed on, it is impossible to stacking in heaps, it is prevented that generate heat, go mouldy.

2.2.6 by the frying production method of traditional tea, become Folium Camelliae sinensis with technique parches such as routine complete, or cake of press or pack evacuation are packed, and are WUJIA CHA of the present invention.

The weight losing function zoopery of WUJIA CHA of the present invention and human experimentation result are shown in experimental example 1 and experimental example 2.

The weight losing function test standard of the i.e. WUJIA CHA of this function tea, carries out according to Ministry of Public Health " health food inspection and assessment technique specification " (2003) year version, and result of the test is as follows:

The animal experiment study of 1 WUJIA CHA weight losing function of experimental example is as follows

1.1 principles

This method is to bring out animal obesity with high heat food, then gives tested material (obese model), observes the weight of animals, the change of body fat content and body health is had harmless.

1.2 instruments

Animal balance, dissecting instrument etc.

1.3 test methods

1.3.1 laboratory animal selects body weight to be the adult rat of 180g �� 200g, male, 100, and purchased from Animal Experimental Study center, Hubei Province, the quality certification number is the dynamic word the 2007-0806th of doctor. Often group 10.

1.3.2 dosage packet tested material (the WUJIA CHA water extract of the present embodiment 1, lower same) with recommended amounts oral 720mg/d every day that is grown up, by adult's 60kg weighing machine, namely 5 times of 12mg/ (kg body weight) are low dose group therein, and 10 times is high dose group. Matched group adopts blank. Tested material gives time totally 4 weeks 30d.

1.3.3 nutrient fodder formula

Normal feedstuff: Fructus Hordei Vulgaris powder 20%, dehydrated vegetable (cabbage of removal moisture) 10%, Semen Glycines powder 20%, yeast 1%, bone meal 5%, Semen Maydis powder 16%, wheat bran 16%, fish flour 10%, Sal 2%.

Nutrient fodder: normal feedstuff 80%, Adeps Sus domestica 10%, yolk powder 10%.

1.3.4 experimental procedure obese model method: after feeding rat 30d with nutrient fodder, the rat of the same age that weight gain is fed than normal diet increases, and difference has significance, then the obese model set up meets requirement of experiment. Obesity rat model is randomly divided into model control group and high and low two tested material test group after setting up. Test group gives the tested material of various dose, and model control group gives coordinative solvent.

1.3.5 observation index body weight, food ration, food utilization, body fat quality (testis and perirenal fat pad).

Table 1 respectively organizes Growth in Rats index and Lipid Metabolism Determination value

* P < 0.05 compared with matched group; It is n=6 that * compares (P < 0.01) a with matched group.

1.3.6 result is as shown in table 1, and high and low dose WUJIA CHA water extract group compares with matched group, and body weight, surrounding genital fat weight average substantially reduce, but zero difference between high and low dose group. Under high power microscope, adipocyte count seen in each visual field, WUJIA CHA water extract group be significantly more than matched group, but cell volume is significantly less than matched group, illustrate that in the adipose cell of WUJIA CHA water extract group, fatty amount is few, together, the cell number therefore seen in the same visual field is more for cell accumulation. Result above shows, this WUJIA CHA water extract really can reduce the accumulation of fat, and once reach effective dose, then escalated dose can not make fat-reducing effect more preferably.

1.4 data process and result judges

The body weight of experimental group and body fat quality, or body weight and fat body ratio are lower than model control group, difference has significance, and food ration is not significantly lower than model control group, and to body without obvious damage, namely can determine that the result of the test of this tested material animal weight losing function is for the positive.

According to " health food inspection and assessment technique specification " (2003 editions) criterion to weight losing function health food, can determine that this WUJIA CHA water extract has antiobesity action.

The human experiment experimentation of 2 WUJIA CHA fat-reducing effects of experimental example is as follows

2.1 principles

To the edible tested material of Simple Obesity experimenter (the WUJIA CHA water extract of the present embodiment 2, lower same), observe body weight, body fat changes of contents and body health had harmless.

2.2 instrument human body component sensing equipments, weighing machine.

2.3 test methods

2.3.1 the selection study subject of experimenter is Simple Obesity crowd. In adult body, total fat percentage reaches man > 25%, the volunteer of female > 30%. Child and teenager actual measurement body weight are above standard the 20% of body weight. Experimenter does not have gallbladder disease.

The serious diseases such as the experimenter that should not participate in the experiment that 2.3.2 we have confirmed that merges intentionally, liver, kidney and hemopoietic system, psychotic; Not by regulation credit tested material, it is impossible to judge that effect or data not umbra ring effect or safety judgement person; Take the article relevant with tested function in a short time, have influence on the judgement person of result.

2.3.3 test requirements document

This WUJIA CHA water extract is the weight losing function beverage not substituting staple food. Do not substitute the weight losing function test of staple food by regulation, adopt control experiment design between own control and group. It is randomly divided into test-meal group and matched group by the body weight of experimenter, body lipid amount, considers the principal element such as age, sex, diet, moving situation etc. that affect result as far as possible, carry out harmonious inspection, with the comparability between guarantee group. Test number often organizes experimenter no less than 10 examples.

2.3.4 the dosage of tested material and using method

This WUJIA CHA water extract is the weight losing function beverage not substituting staple food: test-meal group takes tested material by the instructions of taking recommended, dose, tested material is with recommended amounts oral 720mg/d every day that is grown up, by adult's 60kg weighing machine, namely 12mg/ (kg body weight) takes, 2 times is low dose group therein, and 3 times is high dose group. Matched group adopts blank. Test-meal experiment is carried out by blind. Tested material gives time 30d.

2.3.5 observation index

2.3.5.1 safety indexes

General status includes spirit, sleep, diet, defecation, blood pressure etc., blood, urine, feces routine examination, and Liver and kidney function checks Chest X-rays, electrocardiogram, Abdominal B type ultrasonography inspection (indices checks once before experiment), blood uric acid, urine ketoboidies. Exercise tolerance is tested: exercise tolerance method of testing is cycle ergometer test. Before and after test-meal, experimenter is with identical motion scheme power cycle test, recorded heart rate, and applies the maximal oxygen uptake (L/ divides) of each experimenter of nomography indirect determination of Sstrand and Ryhming. Other untoward reaction are observed: such as anorexia, diarrhoea etc.

2.3.5.2 dietary factors and motion conditions requirement

To experimenter before experiment starts, terminate before carry out 3d dietary survey. In order to get rid of the dietary factor impact on result of the test, it is desirable to consistent with diet as far as possible. The motion of experimenter during experiment is carried out inquiry observe, it is desirable to consistent with daily exercise situation.

2.3.5.3 efficiency index

Before and after experiment, the mensuration of body weight, calculating Body Mass Index, standard body weight, body fat content etc. are in Table 2:

Table 2 respectively organizes human body indicators and Lipid Metabolism Determination value (test number often organizes experimenter 10 example)

* P < 0.05 compared with matched group; Material compares (P < 0.01) with matched group

1.3.6 result is as shown in table 2, and high and low dose WUJIA CHA water extract beverage group compares with matched group, and body weight, fat weight average substantially reduce, but zero difference between high and low dose group. Under high power microscope, adipocyte count seen in each visual field, WUJIA CHA water extract beverage group be significantly more than matched group, but cell volume is significantly less than matched group, illustrate that in the adipose cell of WUJIA CHA water extract beverage group, fatty amount is few, together, the cell number therefore seen in the same visual field is more for cell accumulation. Result above shows, this WUJIA CHA water extract beverage really can reduce the accumulation of fat. Carrying out overall merit according to These parameters measurement result before and after test, wherein body fat content has significance through statistical procedures difference, and to body health without obvious damage. According to " health food inspection and assessment technique specification " (2003 editions) criterion to weight losing function health food, can determine that this WUJIA CHA water extract beverage has antiobesity action.

The safety toxicology experimental conditions that experimental example 3 has weight losing function WUJIA CHA is as follows:

3.1, material

3.1.1 sample human body recommended intake is 5.6g/ (people d), be equivalent to 0.1k/ (kg body weight d) (body mass is in 60kg), WUJIA CHA water extract is this laboratory sample, is configured to the gavage liquid of required trial with distilled water. Adopt the WUJIA CHA that embodiment 1 prepares.

3.1.2 experimental animal ICR kind mice, SD rat of weaning, cleaning grade, medical animal experiment center, Beijing provide. the animal quality certification number: capital doctor examination occupancy permit No. 0608.: BJVS0805.

3.2 methods

3.2.1 each 20 of acute toxicity test mice female, male, 18��22g. Adopt engler (Horm) method suddenly, be randomly divided into 4 groups by body weight, often organize female, male each 5, respectively 1000mg/kg, 4640mg/kg, 10000mg/kg group. WUJIA CHA water extract is given the test agent, is configured to the gavage liquid of desired concn with distilled water. Concrete compound method is as follows: weighs 10.0g WUJIA CHA water extract, adds distilled water and be settled to 40mL, be i.e. 0.5g/mL, and this is high dose group; Other three dosage groups are prepared by that analogy. Animal fasting (be can't help drinking water) after 16h, gives tested material with the capacity gavage of 0.4ml/20g body weight, then observes 7d.

3.2.2 genetic toxicity test

3.2.2.1Ames test TA97a, TA98, TA100, TA102 reference culture and S9 derives from Beijing Disease Prevention and Control Centre, identified Pass Test requirement. adopting conventional panel incorporation methods, test dose is respectively set to 8ug/ ware, 40ug/ ware, 200ug/ ware, 1000ug/ ware, 5000ug/ ware. Concrete compound method is as follows: takes sample 5.00g and adds distilled water to 100mL, for 5000ug/ ware dosage, take this liquid 5mL to add distilled water and be settled to 25mL, for 1000ug/ ware dosage, three below dosage is prepared by that analogy. sample solution with aseptic suction method degerming after, respectively in top agar add 0.1mL test strain enrichment liquid, 0.1mL tested material solution 0.5mLS9 mixed liquor (when needing metabolism activation) mixing after pour on bottom culture medium flat plate. Do untreated control, solvent control and positive control (TA97a [-S9]: 500ug/ ware atabrine simultaneously; TA98 [-S9]; 6.0ug/ ware positive definite toxin; TA100 [-S9] and TA021 [-S9]: 1.0ug/ ware methyl methylsulfonate; TA97a [+S9], TA98 [+S9] and TA100 [+S9]: 10.0ug/ ware 2-aminofluorene; TA102 [+S9]: 50.0ug/ ware 1.8-dihydroxyanthraquinone). a whole set of test repeats again under the same conditions.

3.2.2.2 each 25 of Micronucleus test mice female, male, 25��30g. It is randomly divided into 5 groups by body weight, negative control group (distilled water), WUJIA CHA water extract 2500mg/ (kg body weight d), 500mg/ (kg body weight d) and 10000mg/ (kg body weight d) group, positive control [cyclophosphamide 40mg/ (kg body weight d)] group, female, male each 5. with 2 gavages of 0.4mL/20g body weight (interval 24h), after second time gavage, 6h takes breastbone film-making microscopy.

3.2.2.3 mouse inbred strain male mice 25,25��35g. It is randomly divided into 5 grades by body weight, negative control group (distilled water), WUJIA CHA water extract 2500mg/ (kg body weight d), 500mg/ (kg body weight d) and 10000mg/ (kg body weight d) organize positive control [cyclophosphamide 40mg/ (kg body weight d)] group, female, male each 5. with 0.4mL/20g body weight gavage, continuous 5d, every day 1 time, after gavage first, 35d takes bilateral epididymis film-making microscopy.

3.2.330 sky feeding trial

3.2.3.1 animal and packet ablactation SD rat 80, be randomly divided into 4 groups by body weight, often each 10 of male and female of group. and tested material is mixed through beverage and is given. If: Normal group: give full nutrition and coordinate beverage; Three dosage groups of WUJIA CHA water extract, low dose group: 5.0g/ (kg body weight d) (is equivalent to 50 times of recommended dose), and namely 1kg feedstuff is containing WUJIA CHA water extract 50.0g; Middle dosage group: 7.5g/ (kg body weight d) (is equivalent to 75 times of recommended dose), and namely 1kg feedstuff is containing WUJIA CHA water extract 75.0g; High dose group: 10.0g/ (kg body weight d) (is equivalent to 100 times of recommended dose), and namely 1kg feedstuff is containing WUJIA CHA water extract 100.0g. Animal list cage is raised, and freely takes the photograph water, Continuous Observation 30d, and result is fed.

3.2.3.2 observation index

3.2.3.2.1 ordinary circumstance observes animal once every day.

3.2.3.2.2 body weight upgrowth situation is weighed weekly once.

3.2.3.2.3 food consumption and utilization rate add weekly feedstuff secondary, record of weighing; And record the forage volume scattered and disappeared.

3.2.3.2.4 hematological examination is in testing 30d, and femoral artery takes blood and carries out hemoglobinometry, and erythrocyte, leukocyte, platelet, reticulocyte count and leukocyte differential count measure.

3.2.3.2.5 blood biochemical analysis checks in testing 30d, femoral artery takes blood, separate serum, use Hitachi-7150 automatic clinical chemistry analyzer to carry out total protein (TP), albumin (ALB), glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), glucose (GLU), creatinine (CR), blood urea nitrogen (BUN), cholesterol (T-CHE) and triglyceride (TG) and measure.

3.2.3.2.6 organ weights and histopathologic examination's off-test, big employing femoral artery sacrificed by exsanguination, dissect immediately and carry out gross examination; Weigh liver, spleen, Bilateral Renal and testis, calculate dirty body ratio respectively; With formalin fixing liver, kidney, Stomach duodenum, ovary, testis. Specimens paraffin embedding slices, H.F dyeing carries out histopathologic examination.

3.2.3.2.7 data statistics processing methods experiment the data obtained SAS6.12 software carry out many groups between com-parison and analysis.

3.3 results

3.3.1 acute toxicity test

WUJIA CHA water extract acute toxicity test mouse survival situation of the present invention is in Table 1. its mouse oral LD50 > 10.0g/ (kg body weight d). The no abnormality seen such as animal activity, feed, behavior within the observation period, according to LD50 dose fractionation standard, the nontoxic level in the true border of this sample.

Table 1 nutritional capsule of the present invention acute toxicity test mice relative situation

3.3.2 genetic toxicity test

3.3.2.1Ames test

Capsules A mes result of the test of the present invention in Table 2 and table 3, regardless of whether add S9 mixed liquor, is returned and is become clump count all not more than certainly beaming back 2 times of parameter, and have no dose-response relationship under each concentration of this test. And each positive controls all shows strong mutagenesis. According to Salmonella reversion test result criterion, the WUJIA CHA water extract of this experimental test has no to mutation effect.

Table 2 WUJIA CHA water extract of the present invention first time Salmonella reversion test result

Positive control: be not added with S9:TA97a500.0ug/ ware atabrine; TA986.0ug/ ware rubidomycin; TA100 and TA1021.0ug/ ware methyl methylsulfonate; Add with S9:TA97a, TA98, TA10010.0ug/ ware 2-aminofluorene; TA10250.0ug/ ware 1.8-dihydroxyanthraquinone.

Table 3 WUJIA CHA water extract second time Salmonella reversion test result of the present invention

Positive control is with first time test.

3.3.2.2 Micronucleus test: result of the test is in Table 4. Test group animal is under each test dose, and there are no significant for the difference of polychromatic erythrocyte (PCE) Rate of Appearance Micronuclei, PCE/ mature erythrocyte (RBC) and negative control group, and positive controls shows strong mutagenic action. According to this result of the test, the WUJIA CHA water extract of the present invention of this experimental test has no mutagenic action under 2500mg/ (kg. body weight), 5000mg/ (kg. body weight), 10000mg/ (kg. body weight).

The impact on bone marrow cell micronucleus incidence rate of the table 4 WUJIA CHA water extract

3.3.2.3 mouse inbred strain: result of the test is in Table 5. The rate of teratosperm of negative control group is 1.76%; belong in mice normally deformity scope (normal aberration rate is 0.8%��3.4%); the rate of teratosperm of positive controls (cyclophosphamide group) is 6.20%, and Germ Cells of Male Mice is had strong genetoxic by display.

The rate of teratosperm respectively 1.84%, 1.96%, 1.82% of 3 dosage groups of test. No significant difference with negative control group. Explanation has no WUJIA CHA water extract has genetoxic to mouse sperm.

The impact on mouse sperm deformity rate of the table 5 WUJIA CHA water extract of the present invention

3.3.330 sky feeding trial

3.3.3.1 ordinary circumstance: during whole test, animal hair color is normal, has no dystropy, occurs without dead.

3.3.3.230 nutrition purposes, especially for feeding animals body weights in sky is in Table 6. Each dosage treated animal hypertrophy compares through one factor analysis of variance with matched group, no significant difference (P > 0.05).

The impact on rat body weight (g) of the 30 days feeding trials of table 6 WUJIA CHA water extract

3.3.3.3 food utilization: in rat weightening finish g number/100g beverage, result is in Table 7 and table 8. Each dosage group food utilization compares through one factor analysis of variance with same sex matched group, no significant difference (P > 0.05).

Rat chow utilization rate is affected by table 7 WUJIA CHA water extract for 30 days

The impact on rat each week food utilization of the table 8 WUJIA CHA water extract

3.3.4 hematological examination: test non-phase hemoglobin, erythrocyte, leukocyte, platelet, reticulocyte determination result in Table 9, table 10

30 days feeding trial rat bloods of table 9 WUJIA CHA water extract check result

30 days feeding trial rat bloods of table 10 WUJIA CHA water extract check result

3.3.5 blood biochemistry checking: surveyed the total serum protein (TP) of each treated animal, albumin

(ALB), glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), glucose (GLU), creatinine (CR), blood urea nitrogen (BUN), cholesterol (T-che) and triglyceride (TG) are all in range of normal value. In Table 11, table 12

30 days feeding trial rat blood serum results of biochemical of table 11 WUJIA CHA water extract

30 days feeding trial rat blood serum results of biochemical of table 12 WUJIA CHA water extract

3.3.6 dirty stop than: WUJIA CHA water extract of the present invention the impact of Rats Organs and Tissues absolute mass and dirty body ratio in Table 13, table 14. The internal organs absolute mass of each group and liver weight/body weight thereof, spleen weight/body weight, testis weight/weight ratio compare no abnormality seen with matched group.

The impact on Rats Organs and Tissues absolute mass of the 30 days feeding trials of table 13 WUJIA CHA water extract of the present invention

The impact on the dirty body ratio of rat of the 30 days feeding trials of table 14 WUJIA CHA of the present invention water extract

3.3.7 histopathological examination: during gross anatomy, matched group and WUJIA CHA water extract basic, normal, high three dosage group rat liver,kidney,spleens, Stomach duodenum, ovary, testis observe no abnormality seen at body, therefore only select high dose group to carry out histopathological examination. Under mirror, visible matched group has 3 example rats (female 1 example, male 2 examples) hepatocyte to be mild swelling, it is mild swelling that high dose group has 2 example rats (each 1 example of male and female) hepatocyte to be mild fatty degeneration, 1 example female rats hepatocyte, dilatation and congestion that sinus hepaticus is slight; All the other each example rat hepatocytes have no degeneration necrosis, lobules of liver structural integrity, and sinus hepaticus has no obvious expansion. Renal histopathology inspection shows, matched group has 2 example male rats in 3 example rats (female 2 examples, male 1 example), high dose group, the visible stove cell infiltration of renal interstitial, all the other each example rats have no messangial cell number showed increased, blood capillary tube chamber is without expansion, renal cells, without obvious degeneration necrosis, has no cast. Spleen tissue pathological examination shows, matched group and in test group each example rat be cladded with thin layer connective tissue, spleen is red, white pulp is clear, and central artery occupies acini lienalis central authorities, and acini lienalis and spleen trabeculae all belong to normal. Gastric tissue pathological examination result shows, matched group has 2 example rats (each 1 example of male and female), high dose group to have 3 example rats (female 1 example, male 2 examples), visible glutinous cured epithelial cell partial exfoliation, and having a small amount of cell infiltration, each Rotating fields of all the other rat stomach is clear, and mucosal epithelium is without degeneration necrosis, the intrinsic body of gland of Submucosa is without atrophy, hypertrophy or changes raw, interstitial connective tissues blood vessel is without dilatation and congestion, and muscle layer structure is intact, and serous coat is without inflammatory extractum. Duodenal Tissues pathological examination shows, matched group has 3 example rats (female 1 example, male 2 examples), high dose group to have 2 example rats (each 1 example of male and female), visible mucosal epithelial cells comes off, mucous layer has a small amount of cell infiltration, all the other each Rotating fields of each example are clear, mucosal epithelium is without coming off, and Submucosa has no cell infiltration, and muscle layer structure is intact. Compare between male adult Mus convoluted tubule of testis matched group and high dose group and have no obviously difference. The corpus luteum formed after the follicle of the interior visible different developmental phases of Adult female ovary and ovulation or lean type, compare between matched group and high dose group and have no obvious pathology difference.

Above histopathological examination result is all within normal range, and high dose group compares with blank group and has no obvious biological differences, prompting test agent to liver, kidney, spleen, Stomach duodenum, ovary, testis be without obvious damaging action.

3.4. conclusion

(1) water extract of WUJIA CHA of the present invention, its mouse oral LD50 > 10.0g/ (kg. body weight) female, male. According to LD50 dose fractionation standard, the nontoxic level in the true border of this sample.

(2) negative test result of genetic toxicity test (Salmonella reversion test, Micronucleus test, mouse inbred strain) shows, the water extract of WUJIA CHA of the present invention has no Genotoxic Effect.

(3) rat 30d feeding trial result shows, comparing with matched group, as three dosage group rats 0 of the water extract of WUJIA CHA of the present invention, situation, body weight, food utilization, hematology, blood biochemical analysis, dirty body when histopathologic examination are showed no exception.

We are also by clinical verification, WUJIA CHA of the present invention is except the effect with fat-reducing, moreover it is possible to balancing blood pressure, blood fat reducing, blood sugar lowering, antioxidation, alleviating physical fatigue, slow down aging, reduction headache incidence rate, improve one's memory, also have increase working strength effect. Sutable for men, women, and children. This WUJIA CHA of long-term drink is without any side effects and untoward reaction.

Claims

1. cultivate the method for Cortex Acanthopancis tree and there is the WUJIA CHA of weight losing function for one kind, it is characterised in that: (1) uses as root fertilizer with the residue after the extractions such as medicine food dual purpose plant oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi; (2) from the first two sky that Cortex Acanthopancis tender leaf is plucked, every day, described nutritional solution was formulated by the extracts such as oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi and ginseng distilled water at foliage spray nutritional solution; (3) WUJIA CHA become by the preparation of its Cortex Acanthopancis tree tender leaf, is process for tea manufacture traditionally, forms through artificial parch completely, rather than dry in the sun forms as dry in the sun leaves.

2. method according to claim 1, the residue applied amount after described plant extract is 50-60 kg/acre.

3. method according to claim 1, described root fertilising be by plant extract after residue embedment Cortex Acanthopancis tree root.

4. method according to claim 1, described root fertilizing time is that freeze-up soil is after spring breaks a seal.

5. method according to claim 1, the extract such as described oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi is their distilled water extraction thing, and the time of extraction and number of times be: 60 minutes extraction times first, 40 minutes second extraction time.

6. method according to claim 1, the weight ratio of the extract such as described oolong tea, Poria, Folium Nelumbinis, Fructus Crataegi, Flos Chrysanthemi and ginseng distilled water is 1: 10-12.

7. method according to claim 1, the fountain height of described nutritional solution is 50-80Kg/ mu/sky, and each sprinkling has just started drip with blade face and has been as the criterion.

8. method according to claim 1, the spraying time of described nutritional solution is: the dew in morning carries out after being dried by the sun immediately.

9. have the WUJIA CHA of weight losing function, claim 1-8 either method the tender leaf parch of the Cortex Acanthopancis tree cultivated forms.