CN107467665A - 一种发酵提取油橄榄叶生物活性物质的方法 - Google Patents
一种发酵提取油橄榄叶生物活性物质的方法 Download PDFInfo
- Publication number
- CN107467665A CN107467665A CN201710789257.3A CN201710789257A CN107467665A CN 107467665 A CN107467665 A CN 107467665A CN 201710789257 A CN201710789257 A CN 201710789257A CN 107467665 A CN107467665 A CN 107467665A
- Authority
- CN
- China
- Prior art keywords
- growing leaves
- olive growing
- extraction
- olive
- ferments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000007817 Olea europaea Species 0.000 title claims abstract description 39
- 238000000605 extraction Methods 0.000 title claims abstract description 23
- 230000000975 bioactive effect Effects 0.000 title claims abstract description 16
- 239000000126 substance Substances 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 11
- 230000004151 fermentation Effects 0.000 claims abstract description 11
- 108010059892 Cellulase Proteins 0.000 claims abstract description 8
- 229940106157 cellulase Drugs 0.000 claims abstract description 8
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 5
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 230000002779 inactivation Effects 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000011282 treatment Methods 0.000 claims description 3
- 239000012137 tryptone Substances 0.000 claims description 3
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 claims 1
- 239000011149 active material Substances 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000000470 constituent Substances 0.000 abstract description 3
- 238000004090 dissolution Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000003672 processing method Methods 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 2
- 210000000170 cell membrane Anatomy 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 230000004069 differentiation Effects 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 abstract description 2
- 239000002207 metabolite Substances 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 5
- 235000002725 Olea europaea Nutrition 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000004006 olive oil Substances 0.000 description 2
- 235000008390 olive oil Nutrition 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 208000001953 Hypotension Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明公开了一种发酵提取油橄榄叶生物活性物质的方法，该方法用经少量油橄榄叶提取物诱导培养的枯草芽胞杆菌或酵母菌种子液对油橄榄叶进行发酵处理，利用生物转化手段实现活性物质的有效提取，这种处理方法较之传统提取方法的优点在于简单高效，能耗低，提取效率高，最大程度的保存了活性成分，叶料在纤维素酶的辅助作用下可有效的实现细胞壁的破坏，提高活性成分的溶出率，制得的产品中有效成分含量更高，同时在微生物的作用下还能将一部分活性物质转化为保健功能更为强大的代谢产物，更易被人体吸收利用，实现了油橄榄叶的高值化利用。
Description
技术领域
本发明涉及一种从油橄榄叶中提起活性物质的方法，具体涉及一种发酵提取油橄榄叶生物活性物质的方法。
背景技术
油橄榄是世界上具有悠久历史的一种著名的树种,被誉为世界稀缺高价值资源。油橄榄果实核小、肉厚、含油多,是提取橄榄油的良品，具有较高的使用价值。相对于具有稀缺性的橄榄油，油橄榄叶产量较大，综合利用潜力大，随着现代提取工业及分析技术的发展,从油橄榄叶中分离出多种生物活性成分,如裂环烯醚萜类、黄酮类、甾醇、烷烃类及挥发性成分等。研究表明油橄榄叶提取物具有抗氧化、降血糖、降血压、抗菌抗病毒、抗癌、保护心脑血管等作用。在国外，油橄榄叶及其提取物的保健产品在国外已得到较为充分的开发利用，国内市场也开始出现一些以油橄榄叶及其提取物为原料的保健茶、保健食品及其他保健产品。
目前制备油橄榄叶提起物的方法主要是通过热提取、超声波辅助提取、微波辅助、超临界CO2提取等手段，这些处理方法存在耗能大、耗材多、耗时长、破坏成分、提取率低等缺陷，不利于企业经济效益和产品竞争力的提高，急需研发更为高效的提取手段。
利用微生物发酵炮制中药的方法古已有之，并且是一种相当重要的炮制加工方法，药物里面的活性成分在发酵过程中利用微生物的转化能力来达到改变药性、提高药效、降低毒副作用等功效。《发酵辅助提取对三七总皂苷提取物生物活性的影响》一文揭示了发酵处理能提高三七中有效成分的溶出，稀有皂苷成分增加，清除自由基及抗菌能力更佳，由此表明利用微生物发酵转化技术在生物活性成分提取方面有极大的应有价值。目前油橄榄叶的发酵处理也有一些报道，但是只局限于生产饲料和酿制橄榄酒，经济价值不高。
发明内容
本发明针对已有提起技术的缺陷，提供一种发酵提取油橄榄叶生物活性物质的方法。
本发明是通过以下技术方案实现的：
一种发酵提取油橄榄叶生物活性物质的方法，包括以下步骤：
（1）挑选新鲜健康的油橄榄叶，清洗干净后阴干至含水量≤10%，随后进行粉碎处理，过50目筛，所得粉料灭菌处理后与碳源、氮源、麦芽汁、纤维素酶混匀，接种种子液，在30-35℃条件下发酵10-15天；
（2）发酵结束后过滤留清液备用；
（3）将步骤（2）得到的液体经真空浓缩处理，产物在60-65℃条件下干燥灭活，即得油橄榄叶生物活性提取物。
所述的步骤（1）中粉料、碳源、氮源、麦芽汁、纤维素酶、种子液的重量比为1:0.1:0.2:0.01:0.001:0.1。
所述的种子液是枯草芽孢杆菌孢子悬浮液或酵母菌孢子悬浮液接种到种子培养基中培养得到。
所述的种子液中固形物的体积分数为20-30%。
所述的种子培养基中各组分及其浓度为：胰蛋白胨0.5-1%、葡萄糖2-3%、氯化钠0.1-0.2%、硫酸铵0.1-0.35%、牛肉膏0.01-0.02%、琼脂3-5%、市售油橄榄叶提取物0.01-0.02%，水余量，培养基pH值为7.0-7.5。
本发明用经少量油橄榄叶提取物诱导培养的枯草芽胞杆菌或酵母菌种子液对油橄榄叶进行发酵处理，利用生物转化手段实现活性物质的有效提取，这种处理方法较之传统提取方法的优点在于简单高效，能耗低，提取效率高，最大程度的保存了活性成分，叶料在纤维素酶的辅助作用下可有效的实现细胞壁的破坏，提高活性成分的溶出率，制得的产品中有效成分含量更高，同时在微生物的作用下还能将一部分活性物质转化为保健功能更为强大的代谢产物，更易被人体吸收利用，实现了油橄榄叶的高值化利用。
具体实施方式
一种发酵提取油橄榄叶生物活性物质的方法，包括以下步骤：
（1）挑选新鲜健康的油橄榄叶，清洗干净后阴干至含水量≤10%，随后进行粉碎处理，过50目筛，取100kg粉料灭菌处理后与碳源、氮源、麦芽汁、纤维素酶混匀后接种种子液，在30℃条件下发酵15天，其中粉料、碳源、氮源、麦芽汁、纤维素酶、种子液的重量比为1:0.1:0.2:0.01:0.001:0.1；
（2）发酵结束后过滤留清液备用；
（3）将步骤（2）得到的液体经真空浓缩处理，产物在60℃条件下干燥灭活，即得油橄榄叶生物活性提取物。
其中种子液是枯草芽孢杆菌孢子悬浮液或酵母菌孢子悬浮液接种到种子培养基中培养得到，其中固形物的体积分数为20%。
采用的种子培养基中各组分及其浓度为：胰蛋白胨0.5%、葡萄糖2%、氯化钠0.1%、硫酸铵0.1%、牛肉膏0.01%、琼脂3%、市售油橄榄叶提取物0.01%，水余量，培养基pH值为7.0。
Claims (5)
1.一种发酵提取油橄榄叶生物活性物质的方法，其特征在于，包括以下步骤：
（1）挑选新鲜健康的油橄榄叶，清洗干净后阴干至含水量≤10%，随后进行粉碎处理，过50目筛，所得粉料灭菌处理后与碳源、氮源、麦芽汁、纤维素酶混匀，接种种子液，在30-35℃条件下发酵10-15天；
（2）发酵结束后过滤留清液备用；
（3）将步骤（2）得到的液体经真空浓缩处理，产物在60-65℃条件下干燥灭活，即得油橄榄叶生物活性提取物。
2.如权利要求1所述的一种发酵提取油橄榄叶生物活性物质的方法，其特征在于，所述的步骤（1）中粉料、碳源、氮源、麦芽汁、纤维素酶、种子液的重量比为1:0.1:0.2:0.01:0.001:0.1。
3.如权利要求1或2所述的一种发酵提取油橄榄叶生物活性物质的方法，其特征在于，所述的种子液是枯草芽孢杆菌孢子悬浮液或酵母菌孢子悬浮液接种到种子培养基中培养得到。
4.如权利要求1或2或3所述的一种发酵提取油橄榄叶生物活性物质的方法，其特征在于，所述的种子液中固形物的体积分数为20-30%。
5.如权利要求3所述的一种发酵提取油橄榄叶生物活性物质的方法，其特征在于，所述的种子培养基中各组分及其浓度为：胰蛋白胨0.5-1%、葡萄糖2-3%、氯化钠0.1-0.2%、硫酸铵0.1-0.35%、牛肉膏0.01-0.02%、琼脂3-5%、市售油橄榄叶提取物0.01-0.02%，水余量，培养基pH值为7.0-7.5。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710789257.3A CN107467665A (zh) | 2017-09-05 | 2017-09-05 | 一种发酵提取油橄榄叶生物活性物质的方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710789257.3A CN107467665A (zh) | 2017-09-05 | 2017-09-05 | 一种发酵提取油橄榄叶生物活性物质的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107467665A true CN107467665A (zh) | 2017-12-15 |
Family
ID=60604399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710789257.3A Pending CN107467665A (zh) | 2017-09-05 | 2017-09-05 | 一种发酵提取油橄榄叶生物活性物质的方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107467665A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129124A (zh) * | 2019-05-07 | 2019-08-16 | 青岛科技大学 | 一种香叶油的提取方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676627A (zh) * | 2012-06-05 | 2012-09-19 | 浙江大学 | 三七总皂苷的微生物发酵提取方法 |
| CN106046078A (zh) * | 2016-06-28 | 2016-10-26 | 郭舒洋 | 一种从油橄榄叶中提取橄榄苦苷的方法 |
| CN106822214A (zh) * | 2015-12-03 | 2017-06-13 | 李飞燕 | 一种橄榄叶提取物制备方法 |
-
2017
- 2017-09-05 CN CN201710789257.3A patent/CN107467665A/zh active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676627A (zh) * | 2012-06-05 | 2012-09-19 | 浙江大学 | 三七总皂苷的微生物发酵提取方法 |
| CN106822214A (zh) * | 2015-12-03 | 2017-06-13 | 李飞燕 | 一种橄榄叶提取物制备方法 |
| CN106046078A (zh) * | 2016-06-28 | 2016-10-26 | 郭舒洋 | 一种从油橄榄叶中提取橄榄苦苷的方法 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129124A (zh) * | 2019-05-07 | 2019-08-16 | 青岛科技大学 | 一种香叶油的提取方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103263448B (zh) | 用于发酵预处理改进银杏叶黄酮提取的发酵菌种及应用 | |
| WO2015143783A1 (zh) | 一种天然调理养生酵素及其制造方法 | |
| KR101164529B1 (ko) | 진세노사이드 증대를 위한 인삼발효 방법 | |
| CN104774228B (zh) | 一种从柿子中制备柿单宁的方法 | |
| CN103981054A (zh) | 一种生物酶酿造油茶酒的方法 | |
| CN104256584A (zh) | 一种辣木酵素及其制备方法 | |
| CN106993807A (zh) | 一种生姜酵素的制备方法 | |
| CN104788511A (zh) | 一种从柿子中制备小分子单宁含量多的柿单宁的方法 | |
| CN111135123B (zh) | 一种卷柏发酵提取液的制备方法和应用 | |
| CN103060145A (zh) | 一种蛹虫草葡萄酒的制备工艺 | |
| CN101020911A (zh) | 一种生物学提取方法及其应用 | |
| CN107156822A (zh) | 提高水果酵素中sod的制备方法 | |
| CN111349678A (zh) | 一种油菜花粉多糖提取方法以及提取产物 | |
| CN104651109B (zh) | 一种香蕉风味啤酒的发酵方法 | |
| CN106867739A (zh) | 一种人参蜂蜜葡萄酒及其制备方法 | |
| CN109275819A (zh) | 一种灵芝液体发酵技术及其发酵制品与应用 | |
| CN107467665A (zh) | 一种发酵提取油橄榄叶生物活性物质的方法 | |
| CN110973614A (zh) | 一种连续发酵制备灵芝酵素的方法 | |
| CN106071605A (zh) | 一种灵芝发酵制备酵素的方法 | |
| CN104593199A (zh) | 一种利用丹参药渣酿造保健黄酒的方法 | |
| CN105176724A (zh) | 一种促进免疫、益肾、明目和养颜葡萄酒的生产方法 | |
| CN108669483A (zh) | 一种马尾藻发酵品及其液体发酵制备工艺和应用 | |
| CN112430516B (zh) | 一种发酵莓子酒及其制备方法 | |
| CN114452357A (zh) | 一种含六堡茶具有降血脂降血糖作用的制剂及其制备方法 | |
| CN107183454A (zh) | 一种葛根醋饮料及其制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171215 |