CN107409983B - Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer - Google Patents

Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer Download PDF

Info

Publication number
CN107409983B
CN107409983B CN201710329333.2A CN201710329333A CN107409983B CN 107409983 B CN107409983 B CN 107409983B CN 201710329333 A CN201710329333 A CN 201710329333A CN 107409983 B CN107409983 B CN 107409983B
Authority
CN
China
Prior art keywords
water
seedling raising
fish protein
seedling
soluble fertilizer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710329333.2A
Other languages
Chinese (zh)
Other versions
CN107409983A (en
Inventor
徐兴阳
杨永平
李�杰
欧阳进
端永明
戴恩
高德福
胡卫宗
方正粉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kunming Company of Yunnan Tobacco Co
Original Assignee
Kunming Company of Yunnan Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kunming Company of Yunnan Tobacco Co filed Critical Kunming Company of Yunnan Tobacco Co
Priority to CN201710329333.2A priority Critical patent/CN107409983B/en
Publication of CN107409983A publication Critical patent/CN107409983A/en
Application granted granted Critical
Publication of CN107409983B publication Critical patent/CN107409983B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Fertilizers (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using a fish protein organic water-soluble fertilizer, which comprises the following working procedures of adding water, fertilizing, placing a tray and managing. Firstly, discharging water into a floating seedling raising pond to enable the water depth to be 8-12 cm, and then adding water according to the proportion of 1: and (2) adding the fish protein organic water-soluble fertilizer stock solution into water according to the volume ratio of 1000-6000, then placing the seedling culture tray with the seeds sowed into a floating seedling culture pond, and performing additional fertilization and management according to a conventional floating seedling culture method. The fish protein organic water-soluble fertilizer selected by the invention contains rich nutrients such as protein, peptide, amino acid, vitamins, a large amount of trace elements, enzymes and natural active substances, and is directly added into a water body by adopting a multipoint application method, so that the fertilizer is labor-saving, time-saving and easy to operate, can effectively promote the root development of tobacco seedlings, ensures that the stems are thick and the overground part and the underground part grow in a balanced manner, has certain cold-resisting and disease-resisting capabilities, is suitable for all main cultivated varieties in flue-cured tobacco production, and is particularly suitable for popularization and use in a cultivated area with lower temperature in the seedling stage or severe late spring coldness in the seedling stage.

Description

Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer
Technical Field
The invention belongs to the technical field of tobacco floating seedling, and particularly relates to a floating seedling method for improving the stress resistance and disease resistance of tobacco seedlings by using a fish protein organic water-soluble fertilizer.
Background
In common words: half of the seedlings are harvested. Most of the nutrient components required by the tobacco seedlings in the tobacco floating seedling culture are supplied by the nutrient solution, and the root systems of the tobacco seedlings absorb nutrients and moisture from the nutrient solution through the matrix, so that the fertilizer components and the proportion applied in the floating seedling culture nutrient solution have very important significance for promoting the healthy growth of the tobacco seedlings, improving the stress resistance and the disease resistance of the tobacco seedlings and improving the transplanting survival rate. (1) With the increasing concern of tobacco pesticide residue by more and more consumers and the increasingly strict food safety laws and regulations of governments, the application of traditional chemical fertilizers in tobacco cultivation is gradually limited, and natural fertilizer products are more and more valued by producers and consumers; (2) the late spring coldness of the Kunming tobacco area exists all the year round in the seedling growing period, and the temperature of the whole seedling growing period of the northern region such as the region of seeking a pasture, salary and the like is lower, the late spring coldness is more prominent, the healthy growth of tobacco seedlings is not facilitated, and the seedling quality of the tobacco seedlings is even seriously influenced, so far, no effective method exists; (3) tobacco seedling raising is intensive tobacco seedling, the seedling raising field is highly concentrated, and after the tobacco seedling raising through chemical fertilizers, a large amount of fertilizers with residual nutrients are contained in water and are often poured on the spot or discharged into rivers, so that the soil is damaged, the rivers are polluted to a certain extent, and the environmental protection pressure is increased. The fish protein organic water-soluble fertilizer is prepared by performing biochemical extraction on the unique menhaden in the Atlantic ocean at low temperature, contains rich proteins, peptides, amino acids, vitamins, a large amount of trace elements, enzymes and natural active substances, is a novel functional liquid fertilizer product, and can generate the effects of resisting cold, resisting diseases, promoting growth and development and the like after being absorbed by crops (tobacco). However, if the organic water-soluble fertilizer is not applied properly, the organic water-soluble fertilizer has poor absorption of nutrient elements and limited improvement on the quality of tobacco seedlings, and the production cost is increased and the organic water-soluble fertilizer is wasted. Therefore, the method for screening and reasonably applying the fish protein organic water-soluble fertilizer on the tobacco is very important.
Disclosure of Invention
The invention aims to provide a floating seedling method for improving the stress resistance and disease resistance of tobacco seedlings by using a fish protein organic water-soluble fertilizer.
The purpose of the invention is realized as follows: the floating seedling raising method for improving the stress resistance and disease resistance of tobacco seedlings by using the fish protein organic water-soluble fertilizer comprises the following working procedures of water adding, fertilization, tray placing and management, and specifically comprises the following steps:
A. adding water: discharging water into the floating seedling raising pond to enable the water depth to be 8-12 cm;
B. fertilizing: according to the fish protein organic water-soluble fertilizer: water =1: adding a fish protein organic water-soluble fertilizer stock solution into water at a volume ratio of 1000-6000;
C. placing a plate: putting the seedling raising plate with the sowed seeds into a floating seedling raising pool;
D. management: topdressing and management are carried out according to a conventional floating seedling method.
Compared with the prior art, the invention has the following beneficial effects:
the fish protein organic water-soluble fertilizer selected by the invention is prepared from raw materials of the special menhaden in the Atlantic ocean by biochemical extraction at low temperature, contains rich nutrient substances such as protein, peptide, amino acid, vitamin, a large amount of trace elements, enzyme, natural active substances and the like, and is a novel functional liquid fertilizer product. Compared with the application method of spraying the leaf surfaces and mixing the substrate in the seedling stage, the method adopts a multipoint application method to directly add the tobacco into the water body, thereby not only saving labor and time and being easy to operate, but also effectively promoting the root system development of the tobacco seedlings, ensuring the stems to be thick and strong, and the overground part and the underground part to grow in a balanced way, and the tobacco seedlings have certain cold-resisting and disease-resisting capabilities, are suitable for all main cultivated varieties in the production of flue-cured tobacco, and are particularly suitable for being popularized and used in the cultivated areas with low temperature in the seedling stage or severe late spring coldness.
Detailed Description
The present invention is further illustrated but not limited in any way by the following description, and any alterations or substitutions based on the teachings of the present invention are intended to fall within the scope of the present invention.
The invention relates to a floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using a fish protein organic water-soluble fertilizer, which comprises the following working procedures of water adding, fertilization, tray placing and management, and specifically comprises the following steps:
the water adding process comprises the following steps: discharging water into the floating seedling raising pond to enable the water depth to be 8-12 cm;
the fertilizing process comprises the following steps: according to the fish protein organic water-soluble fertilizer: water =1: adding a fish protein organic water-soluble fertilizer stock solution into water at a volume ratio of 1000-6000;
the tray placing process comprises the following steps: putting the seedling raising plate with the sowed seeds into a floating seedling raising pool;
the management process comprises the following steps: topdressing and management are carried out according to a conventional floating seedling method.
The water adding process is that the water discharging depth of the floating seedling raising pond is preferably 10 cm.
In the fertilization procedure, the volume ratio of the fish protein organic water-soluble fertilizer to water is preferably 1: 2000-5000. Adopting a multipoint adding method and stirring uniformly.
In the fertilizing procedure, the organic matter of the fish protein organic water-soluble fertilizer stock solution is more than or equal to 32%, the pH value is 3.0-5.0, and the water-insoluble substance is less than or equal to 2%. The fish protein organic water-soluble fertilizer stock solution is a 'good-stand' fish protein organic water-soluble fertilizer, the pH value is 3.0-5.0, the organic matter is more than or equal to 32%, the total nitrogen content is more than or equal to 4.50%, the total phosphorus content is more than or equal to 0.3%, the total potassium content is more than or equal to 1.5%, the total free amino acid content is more than or equal to 4.0%, the total copper content is less than or equal to 3.0mg/kg, the total zinc content is more than or equal to 15.0mg/kg, the total iron content is less than or equal to 450mg/kg, the total manganese content is more than or equal to 5.0mg/kg, the total molybdenum content is more than or equal to 0.01mg/kg, and the total boron. The content of organic nitrogen, phosphorus and potassium is more than or equal to 97 percent, and the content of organic medium trace elements is more than or equal to 95 percent.
The seeds in the tray placing procedure can be any one of all main cultivars in flue-cured tobacco production, can be Honghuadajinyuan or KRK26 with high utilization rate of matrix fertilizer, and can also be K326, Yunyan 87, Yunyan 97 or Yunyan 203 with low utilization rate of matrix fertilizer.
Management procedure the topdressing refers to: applying 24.000-25.500 g of special compound fertilizer for seedling cultivation to each seedling cultivation plate of the seedlings under the film; 48.000-51.000 g of special compound fertilizer for additional application of seedling for each seedling raising plate in conventional seedling raising, 0.800-0.850 kg and 1.600-1.700 kg of special compound fertilizer for additional application of seedling raising for each pool based on 32 seedling raising plates, which is equivalent to 0.144-0.153 kg and 0.288-0.306 kg of pure nitrogen respectively.
The length and width of the seedling raising plate are 66.5cm multiplied by 34 cm.
The special compound fertilizer for seedling culture is a seedling fertilizer with a formula of 'rainbow leaves' brand tobacco floating seedling culture.
Example 1
1.0 ton of water is discharged into the floating seedling raising pond to enable the water depth to be 10cm, and then the water is discharged into the floating seedling raising pond according to the volume ratio of 1:2000, adding the original solution of the 'Harden' fish protein organic water-soluble fertilizer into water by adopting a multipoint adding method, and uniformly stirring. The pH value of the 'Harden' fish protein organic water-soluble fertilizer is 4.26, the organic matter is 44.94%, the total nitrogen content is 4.90%, the total phosphorus content is 0.33%, the total potassium content is 1.66%, the total amount of free amino acids is 4.09%, the total copper content is 2.66mg/kg, the total zinc content is 15.16mg/kg, the total iron content is 419.98mg/kg, the total manganese content is 18.21mg/kg, the total molybdenum content is 0.11mg/kg, and the total boron content is 9.51 mg/kg. Next, a seedling raising plate sown with the Honghuadajinyuan seeds is placed into a floating seedling raising pool, and the length and width of the seedling raising plate are 66.5cm multiplied by 34 cm. And then topdressing and management are carried out according to a conventional floating seedling method. Namely, 24.000g of special compound fertilizer for seedling dressing is applied to each seedling raising plate of the seedlings under the film; 48.000g of special compound fertilizer for additional application of seedling for each seedling-raising plate in conventional seedling raising, 0.800kg and 1.600kg of special compound fertilizer for additional application of seedling raising for each pool based on 32 seedling-raising plates, which is equivalent to 0.144kg and 0.288kg of pure nitrogen respectively.
Example 2
0.6 ton of water is discharged into the floating seedling raising pond to enable the water depth to be 8cm, and then the water is discharged into the floating seedling raising pond according to the volume ratio of 1: 1000, adding the original solution of the good-standing fish protein organic water-soluble fertilizer into water by adopting a multipoint adding method, and uniformly stirring. The effective components of the 'Haridren' fish protein organic water-soluble fertilizer are the same as example 1. Next, a seedling raising tray on which seeds of yunyan 87 were sown was placed in the floating seedling raising pond, and the length and width of the seedling raising tray were the same as those of example 1. And then topdressing and management are carried out according to a conventional floating seedling method. The top dressing scheme was the same as in example 1.
Example 3
1.2 tons of water are discharged into the floating seedling raising pond to ensure that the water depth is 12cm, and then the water is discharged into the floating seedling raising pond according to the volume ratio of 1: 5000, adding the original liquid of the good-standing fish protein organic water-soluble fertilizer into water by adopting a multipoint adding method, and uniformly stirring. The effective components of the 'Haridren' fish protein organic water-soluble fertilizer are the same as example 1. Next, a seedling raising tray on which K326 seeds were sown was placed in a floating seedling raising pond, and the length and width of the seedling raising tray were the same as those of example 1. And then topdressing and management are carried out according to a conventional floating seedling method. The top dressing scheme was the same as in example 1.
Example 4
Test materials: the test was carried out at the seek Dian scientific test base of Kunming City, Nicotiana, Yunnan province. The flue-cured tobacco variety used is Yunyan 203, the organic fertilizer is 'Haoliden' fish protein organic water-soluble fertilizer, purchased from Piliton agricultural science and technology (Nanjing) Limited, the domestic registration number is agricultural fertilizer (2015) standard 4670, which belongs to soil fertilizing and improving substances, and the active ingredients are detailed in the detection result of 'Haoliden' fish protein organic water-soluble fertilizer in Table 1. The test time is 2016 years from 8 months to 9 months.
Table 1 results of organic water-soluble fertilizer test for heilideng fish protein (Yunnan three-label agriculture and forestry science and technology limited, 2017)
Figure DEST_PATH_IMAGE002
Processing and setting: the experiment is carried out by adopting 3 modes of spraying on leaf surfaces, mixing matrix during sowing and adding water during sowing, setting 5 treatments, additionally setting 2 control groups in a polypeptide protection and conventional mode, and totally 7 treatments. The method comprises the following specific steps:
a1: preparing a fish protein organic water-soluble fertilizer: water =1:200, spraying for 5-7 times in a seedling emergence period for 1 time, and continuously for 3 times;
a2: preparing a fish protein organic water-soluble fertilizer: water =1: 300, spraying for 5-7 times in 5-7 days for 1 time, and continuously for 3 times;
a3: preparing a fish protein organic water-soluble fertilizer: water =1: mixing according to the volume ratio of 400, spraying for 5-7 days for 1 time at the emergence stage, and continuously for 3 times;
a4: preparing a fish protein organic water-soluble fertilizer: water =1: 100 volume ratio, and mixing the mixture into a matrix;
a5: and (3) performing organic water-soluble fertilizer preparation on the fish protein in a sowing period: water =1: 5000, and putting the mixture into a water body (the concentration is 0.02%);
a6 (CK 1): the polypeptide is mixed with the matrix, and each plant is 0.2 g (200 g is needed for each mu of seedlings).
A7 (CK 2): and (4) conventionally culturing the fish fries without applying polypeptide to protect fish protein.
The seedling raising plates with 162 holes are used for floating seedling raising, 1 pond (6 ponds) is treated for each treatment, the substrate with nutrient solution fertilizer is uniformly applied in the seedling emergence stage, the pest control is unified, the illumination is reasonably adjusted (the net is covered and uncovered at proper time), and other measures are managed according to the strong seedling culture standard.
The test method comprises the following steps: recording the sowing period, the emergence period and the time for spraying the fish protein organic water-soluble fertilizer each time; the effect records include: after spraying for 3 times (equivalent to 20 days after emergence), investigation is carried out, and the conditions of tobacco plant uniformity, growth and development (weight of overground part and underground part), tobacco seedling color and the like are compared; and investigating stem height, stem circumference and seedling rate during transplanting. According to the above process, the comprehensive effect evaluation is performed on each treatment of the test. Recording a standard reference tobacco agronomic trait investigation measuring method YCT 142-2010.
And (3) test results:
1. different treatment of tobacco seedling growth period performance
As can be seen from Table 2, there was some variation in the growth period of tobacco seedlings between treatments. Compared with 3 modes of foliage spraying, matrix mixing during sowing and water body adding during sowing, when the growth and development of tobacco seedlings are observed to be in the cat ear period, the modes of directly adding the water body (A5), matrix mixing (A4) and foliage spraying (A1, A2 and A3) are carried out in sequence, and the growth progress of the 'directly adding the water body' is equivalent to that of the control 1.
TABLE 2 observation and record of growth period of seedling stage
Figure DEST_PATH_IMAGE004
2. Quality performance of tobacco seedlings treated differently
As can be seen from Table 3, there was some difference in the quality of tobacco seedlings between the different treatments. The root system of the treated A5 (directly added into water) is most developed and superior to that of two controls and other treatments; the roots were weakest with treatment a3 (400-fold spray) and were lower than the two control and other treatments. Treatment a5 was inferior to control 2 only in terms of total biomass. Thus, the tobacco seedlings treated in the stage A5 are the most robust, and the overground part and the underground part grow coordinately, so that the tobacco seedlings have a material basis for forming strong seedlings.
TABLE 3 tobacco seedling quality index statistics in seedling stage (first leaf-cutting day)
Figure DEST_PATH_IMAGE006
Remarking: measuring date of 9 months and 20 days
3. Quality performance of seedling stage of different treatments
As seen from Table 4, there was some difference in the quality of tobacco seedlings between treatments at the seedling stage. The stem circumference of the treated A5 (directly added into water) is the largest, the variation degree is smaller, and the stem circumference is superior to that of two controls; the stem is highest in comparison 2, and the degree of variation is largest; the seedling rate is equivalent to A5, A6 and A7, and reaches over 83 percent. In conclusion, the tobacco seedlings treated by the method A5 have medium stem height, thick stems, good uniformity, high seedling rate and best comprehensive quality.
TABLE 4 tobacco seedling quality statistics in seedling stage
Figure DEST_PATH_IMAGE008
Remarking: measuring date of 9 months and 20 days
1) Stem height and stem thickness, selecting representative 10 plants for each treatment, and calculating an average value after measurement;
2) the seedling rate, namely the proportion of tobacco seedlings which can be transplanted, is calculated by selecting 3 trays for observation.
Experiments of 3 application modes (foliage spraying, matrix mixing during sowing and water body adding during sowing) of the 'Haierden' fish protein organic water-soluble fertilizer show that the effects of foliage spraying and matrix mixing during seedling stage are poor, labor and time are wasted, the effect of cultivating flue-cured tobacco strong seedlings by directly adding water body is good, the developed root system, the stout stems and the balanced growth of overground parts and underground parts can be promoted, and the fertilizer is labor-saving and easy to operate and is an ideal choice.
Example 5
Test materials: the test was carried out at the seek Dian scientific test base of Kunming City, Nicotiana, Yunnan province. The flue-cured tobacco variety is Yunyan 203, the organic fertilizer is 'Haoren' fish protein organic water-soluble fertilizer, the fertilizer is purchased from Piliton agricultural science and technology (Nanjing) Limited company, and the active ingredients of the fertilizer are detailed in a table 1 of a detection result of the Haoren fish protein organic water-soluble fertilizer. The test time is 2016 years 10 months to 12 months.
Processing and setting: the test totaled 6 treatments. The method comprises the following specific steps:
t1: preparing a fish protein organic water-soluble fertilizer: water =1:200, spraying the mixture 3-4 days after final singling, 1 time in 5-7 days, and 3 times continuously;
t2: and (3) performing organic water-soluble fertilizer preparation on the fish protein in a sowing period: water =1: 5000, and putting the mixture into a water body (the concentration is 0.02%);
t3: and (3) performing organic water-soluble fertilizer preparation on the fish protein in a sowing period: water =1:2000 volume percent, putting into water (the concentration is 0.05 percent);
t4: and (3) performing organic water-soluble fertilizer preparation on the fish protein in a sowing period: water =1: 8000 percent, and putting the mixture into a water body (the concentration is 0.01 percent);
t5 (CK 1): mixing the polypeptide (special for seedling stage) with a matrix, wherein the dosage is 0.15-0.2 g per plant;
t6 (CK 2): and (4) conventionally culturing the fish fries without applying polypeptide to protect fish protein.
Sowing the seeds in 10 months and 26 days. And (3) carrying out floating seedling raising by using 325-hole seedling raising plates, uniformly applying a self-contained nutrient solution fertilizer to the substrate in the seedling emergence stage in each treatment 1 pond (6 plates), uniformly preventing and treating insect pests, and managing other measures according to strong seedling culture standards.
The test method comprises the following steps:
1. physiological and biochemical detection of tobacco seedling quality:
detection indexes are as follows: the leaf and root system are selected to separately detect 6 physiological and biochemical indexes from 1 heart of 6 leaves to 1 heart of 7 leaves of tobacco seedlings. Including chlorophyll (chlorophyll a, chlorophyll b) content, proline (Pro), Malondialdehyde (MDA), amylase Activity (AMY), superoxide dismutase (SOD), and Nitrate Reductase (NR) activity.
The sampling method comprises the following steps: sampling from 8 to 9 am, storing in a curling stone, and performing sample preparation and detection (after the sample is processed, storing in a refrigerator at 2 ℃).
a. Root system sampling: 20 strains were taken per dish, and 1 sample was taken per 10 strains. The root system was washed clean, and the root tip was taken 1/3 for each plant, the root system of 10 tobacco plants per sample.
b. Leaf sampling: 20 strains were taken per dish, and 1 sample was taken per 10 strains. 1 leaf per plant, and 10 leaves per sample, the leaf tip 1/5 of the 2 nd leaf below the heart leaf (less than 1 cm).
2. Observation and recording:
a standard reference tobacco agronomic trait investigation and measurement method YCT142-2010 and a tobacco pest classification and investigation method GB/T23222-2008 are recorded.
And (3) test results:
1. physiological and biochemical indexes of quality of tobacco seedlings in seedling stage
As shown in table 5, compared with the control group without applying the fish protein organic water-soluble fertilizer, the differences of the 4 indexes except the malonaldehyde content and the chlorophyll content are smaller among the 6 physiological and biochemical indexes. Wherein, the proline content (reflecting the stress degree of the tobacco seedlings under the adverse circumstances, the numerical value is larger and the stress degree of the tobacco seedlings under the adverse circumstances is larger) is lower than that of the control group, which indicates that the tobacco seedlings treated by the fish protein organic water-soluble fertilizer are lower in the stress degree of the tobacco seedlings under the adverse circumstances; the amylase activity (reflecting the accumulation degree of dry matters, the larger the numerical value is), and the nitrate reductase activity (reflecting the activities of leaves and roots, the larger the numerical value is, the stronger the activity is) exceed those of the control group, which indicates that the dry matters of the tobacco seedlings treated by the fish protein organic water-soluble fertilizer are accumulated more, and the activities of the roots and the leaves are stronger. Therefore, the fish protein organic water-soluble fertilizer obviously enhances the adaptability, the vitality and the dry matter accumulation of the tobacco seedlings and creates favorable conditions for cultivating healthy disease-free strong seedlings.
TABLE 5-1 detection results of physiological and biochemical indexes of tobacco seedlings in seedling stage
Figure DEST_PATH_IMAGE010
Remarking: chlorophyll unit is mg.g-1.FW。
TABLE 5-2 detection results of enzyme Activity indexes of tobacco seedlings in seedling stage
Figure DEST_PATH_IMAGE012
Remarking: the superoxide dismutase unit is U g-1min-1(ii) a Nitrate reductase units of NO2-μg.g-1FW.h-1
2. Seedling stage application mode verification test result
As can be seen from Table 6, there was some difference in the quality of tobacco seedlings between the different treatments. The comprehensive performance is best for treating T3, the root system is equivalent to polypeptide protector (CK 1), but the ratio of root cap is superior to CK 1; the second was T2, while the remaining treatments showed poor root development. Therefore, the effect of adding the 'Harden' fish protein organic water-soluble fertilizer into the water body is superior to the effect of spraying the foliage, and the ratio of the water body to the water body is 1: preferably 2000-5000, and is beneficial to cultivating strong seedlings.
TABLE 6 tobacco seedling quality index statistics in seedling stage (4 leaves 1 heart-5 leaves 1 heart)
Figure DEST_PATH_IMAGE014
Example 6
Test materials: the same as in example 5.
Processing and setting: the test totaled 2 treatments. The method comprises the following specific steps:
SF: and (3) performing organic water-soluble fertilizer preparation on the fish protein in a sowing period: water =1: 5000 times of the solution is put into water (the concentration is 0.02%);
CK: and (4) conventionally culturing the fish fries without applying polypeptide to protect fish protein.
Sowing the seeds in 11 months and 4 days. The SF treatment is that a 'good-stand' fish protein organic water-soluble fertilizer is additionally applied on the basis of conventional seedling culture, and the water concentration is 0.02 percent; and the floating seedling culture is carried out on the 2 treatments by using a 162-hole seedling culture plate.
The test method comprises the following steps: the same as in example 5.
And (3) test results: as seen from Table 7, the total biomass of the tobacco seedlings applied with the fish protein organic water-soluble fertilizer exceeds that of the control group, the root system has obvious advantages, the overground part has slight advantages, and the root cap ratio is good; on the other hand, the width of the blade is increased, the length of the blade is reduced, and the blade shape is wider and round. Therefore, the test result further proves the seedling strengthening effect of the 'Harden' fish protein organic water-soluble fertilizer.
TABLE 7 tobacco seedling quality index statistics in seedling stage (4 leaves 1 heart-5 leaves 1 heart)
Figure DEST_PATH_IMAGE016

Claims (5)

1. A floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using a fish protein organic water-soluble fertilizer is characterized by comprising the following working procedures of water adding, fertilizer application, tray placement and management, and specifically comprises the following steps:
A. adding water: discharging water into the floating seedling raising pond to enable the water depth to be 8-12 cm;
B. fertilizing: adding a fish protein organic water-soluble fertilizer stock solution into water according to the volume ratio of the fish protein organic water-soluble fertilizer to the water =1: 2000-5000; the fish protein organic water-soluble fertilizer raw solution has the organic matter content of more than or equal to 32 percent, the pH value of 3.0-5.0, the water-insoluble substance content of less than or equal to 2 percent, the total nitrogen content of more than or equal to 4.50 percent, the total phosphorus content of more than or equal to 0.3 percent, the total potassium content of more than or equal to 1.5 percent, the total free amino acid content of more than or equal to 4.0 percent, the total copper content of less than or equal to 3.0mg/kg, the total zinc content of more than or equal to 15.0mg/kg, the total iron content of less than or equal to 450mg/kg, the total manganese content of more than or equal to 8.0mg/kg, the total molybdenum content of;
C. placing a plate: putting the seedling raising plate with the sowed seeds into a floating seedling raising pool; the seed is any one of Honghuadajinyuan, K326, Yunyan 87, KRK26, Yunyan 97 and Yunyan 203;
D. management: topdressing and management are carried out according to a conventional floating seedling method.
2. The floating seedling raising method according to claim 1, wherein the depth of the water discharged from the floating seedling raising pond in the step A is 10 cm.
3. A float seedling method according to claim 1, characterized in that the stock solution of the fish protein organic water-soluble fertilizer in the step B is added by a multipoint adding method and is stirred uniformly.
4. A floating seedling raising method according to claim 1, wherein the additional fertilizer in the step D is: applying 24.000-25.500 g of special compound fertilizer for seedling cultivation to each seedling cultivation plate of the seedlings under the film; 48.000-51.000 g of special compound fertilizer for additional application of seedling for each seedling raising plate in conventional seedling raising, 0.800-0.850 kg and 1.600-1.700 kg of special compound fertilizer for additional application of seedling raising for each pool based on 32 seedling raising plates, which is equivalent to 0.144-0.153 kg and 0.288-0.306 kg of pure nitrogen respectively.
5. A floating seedling raising method according to claim 4, wherein the length and width of the seedling raising tray in the step D are 66.5cm x 34 cm.
CN201710329333.2A 2017-05-11 2017-05-11 Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer Active CN107409983B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710329333.2A CN107409983B (en) 2017-05-11 2017-05-11 Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710329333.2A CN107409983B (en) 2017-05-11 2017-05-11 Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer

Publications (2)

Publication Number Publication Date
CN107409983A CN107409983A (en) 2017-12-01
CN107409983B true CN107409983B (en) 2020-05-12

Family

ID=60425900

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710329333.2A Active CN107409983B (en) 2017-05-11 2017-05-11 Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer

Country Status (1)

Country Link
CN (1) CN107409983B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111165517A (en) * 2019-12-29 2020-05-19 山东劲脉植物细胞信息技术有限公司 Natural organic-inorganic anti-freezing preparation and production method thereof
CN111187119A (en) * 2020-03-16 2020-05-22 河南中烟工业有限责任公司 Leaf fertilizer for improving disease resistance of flue-cured tobacco
CN113349227B (en) * 2021-05-12 2022-06-17 湖南省烟草公司株洲市公司 Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method
CN113287506A (en) * 2021-06-15 2021-08-24 河南农业大学 Method for improving freezing resistance of tobacco seedlings

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101811894A (en) * 2010-04-06 2010-08-25 中国农业大学 Preparation and seedling methods of nutrient solution and seedling medium for organic floating seedling of tobacco
CN102627492A (en) * 2012-05-03 2012-08-08 昆明东燃科技开发有限公司 Tobacco floating seedling culture nutrient solution and seedling culture method by using same
CN103891588A (en) * 2014-04-04 2014-07-02 云南省烟草公司楚雄州公司 Flue-cured tobacco float seedling breeding method using novel substrate

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101811894A (en) * 2010-04-06 2010-08-25 中国农业大学 Preparation and seedling methods of nutrient solution and seedling medium for organic floating seedling of tobacco
CN102627492A (en) * 2012-05-03 2012-08-08 昆明东燃科技开发有限公司 Tobacco floating seedling culture nutrient solution and seedling culture method by using same
CN103891588A (en) * 2014-04-04 2014-07-02 云南省烟草公司楚雄州公司 Flue-cured tobacco float seedling breeding method using novel substrate

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
不同有机营养液对烟苗生长发育及生理特性的影响;赵会纳等;《河南农业科学》;20130131;第42卷(第1期);第38-42页 *

Also Published As

Publication number Publication date
CN107409983A (en) 2017-12-01

Similar Documents

Publication Publication Date Title
CN105027940B (en) A kind of Rocky Desertification Region herbage planting and establishing method and its application method
CN107409983B (en) Floating seedling raising method for improving stress resistance and disease resistance of tobacco seedlings by using fish protein organic water-soluble fertilizer
Esakkiammal et al. Studies on the combined effect of vermicompost and vermiwash prepared from organic wastes by earthworms on the growth and yield parameters of Dolichous lab lab
CN109315247B (en) Method for producing selenium-rich rice by using astragalus sinicus and rape activated soil
CN105638025A (en) Anthocephalus chinensiss seedling culture method
Ng’etich et al. Effects of composted farmyard manure on growth and yield of spider plant (Cleome gynandra)
CN109258325B (en) Alkaloids-blume planting method in alpine region
CN110476739B (en) Method suitable for planting capsicum in Hainan province
CN105519398B (en) Container seedling raising method for special valuable tree species corbicula fluminea at karst
JP2000224928A (en) Cultivation of rice
Hossen et al. effect of mat type rice seedling growing media on block formation and fungal infection.
CN105104090A (en) Walnut-tree cultivation method
CN109937814A (en) A kind of implantation methods of passion fruit
Ng'etich et al. Growth, yield and physiological responses of spider plant (Cleome gynandra L.) to calcium ammonium nitrate rates.
CN107409984B (en) Floating seedling method for cultivating healthy strong seedlings by using biological organic liquid fertilizer
Sardoei et al. Effects of zinc sulphate and ascorbic acid on flowering characteristics of ornamental plant Gazania (Gazania rigens) cv. Daybreak Red Stripe
CN104620920B (en) A kind of greenhouse production method efficient, sustainable and fruit tree potting media
CN107011032B (en) Mushroom residue composite matrix for cultivating organic watermelon seedlings
Pegu et al. Performance of some blackgram genotypes in relation to physio-chemical, root parameters and yield as influence by foliar feeding with boron
Jawaharlal et al. Comparative analysis of conventional and precision farming systems for African marigold (Tagetes erecta L.)
Eliyanti et al. Improving drought tolerance in chili pepper by the application of local microorganisms (LMo)
Sadaqa et al. Allelopathic activity of some common weeds species in onion fields
CN107484539B (en) Method for maintaining soil fertility and controlling heavy metal content in soil by organic corn production system
Hidayah et al. Katokkon chili Livestock Manure RESPONSE OF GROWTH AND YIELD OF KATOKKON CHILI PLANTS (Capsicum chinense JACQ.) TO THE TYPE AND DOSAGE OF ANIMAL MANURE FERTILIZER
Thakur et al. Effect of different soil agro-techniques on leaf nutrient status of peach grown in a replant sick soil

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant