CN107298601A - A kind of preparation method of compound marine alga fertilizer - Google Patents
A kind of preparation method of compound marine alga fertilizer Download PDFInfo
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- CN107298601A CN107298601A CN201710356940.8A CN201710356940A CN107298601A CN 107298601 A CN107298601 A CN 107298601A CN 201710356940 A CN201710356940 A CN 201710356940A CN 107298601 A CN107298601 A CN 107298601A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention belongs to alga fertilizer field, and in particular to a kind of preparation method of compound marine alga fertilizer.The preparation method of the compound marine alga fertilizer of the present invention includes the techniques such as pretreatment, enzymolysis, microbiological treatment, drying.The present invention is handled fresh feed by the way of cryogenic freezing is broken, effectively protects marine alga effective active composition, makes the alga fertilizer of gained nutritious.The present invention is using mode associated with a variety of enzymes, and macromolecular carbohydrate, proteinaceous components in being homogenized to marine alga are effectively degraded, and improve the extraction efficiency of DDGS in marine alga.The present invention prepares compound marine alga fertilizer using compound microorganism ferments are digested, so as to retain the active component of marine alga to greatest extent, the effect of alga fertilizer is improved, with preferable application prospect.
Description
Technical field
The invention belongs to alga fertilizer field, and in particular to a kind of preparation method of compound marine alga fertilizer.
Background technology
Marine alga is the general name of the marine algaes such as sea-tangle, seaweed, undaria pinnitafida, agar, is submarine algae, is to plant
Had a large amount in variety in the cryptogam of thing circle, algae and energy is all produced with photosynthesis.The species of marine alga much includes brown alga, red
Algae, green alga, common brown alga include kelp, sargassum and Fucus, and common red algae includes dulse, purple
Dish, Gelidium, Chondrus.Brown alga in marine alga is rich in polysaccharose substance, such as:Algin, fucoidin and sea-tangle are formed sediment
Powder etc., these polysaccharose substances are that many land plants are lacked.Algin is by mannuronic acid and guluronic acid two
The block linear polymer of kind of monomer composition, it has gel, chelating and the characteristic such as hydrophilic, and it changes soil by these characteristics
Some properties of earth, can be used as soil conditioner.After biochemical treatment, used as new-type fertilizer, with volume increase,
Mitigate the effects such as residual, the improvement local flavor of disease, reduction medicine.
Produce marine alga fertilizer using marine alga, critical technological point cellulose, pectic substance etc. mainly in marine alga is solved it is main into
Divide on the problem of being difficult to degraded, while retaining the active ingredients such as nutrient, plant hormone in marine alga to greatest extent.Mesh
Before, the main method using chemical degradation of processing of domestic alga fertilizer is more to effective active composition destruction in marine alga, after influence
The effect of phase fertilizer.
The content of the invention
It is an object of the invention to provide a kind of preparation method of compound marine alga fertilizer, using digesting compound microorganism ferments
Compound marine alga fertilizer is prepared, so as to retain the active component of marine alga to greatest extent, the effect of alga fertilizer is improved, with preferable
Application prospect.
The present invention is achieved using following technical scheme:
A kind of preparation method of compound marine alga fertilizer, specifically includes following steps:
(1) pre-process:Fresh brown alga, red algae, green alga, sargassum, bladder-wrack, clean repeatedly, filter out impurity, dehydration is extremely
Water content is 40~50wt%, is placed in 4~8h of freezing at -15 DEG C to -25 DEG C;Historrhexis's crusher machine is used after taking-up, by feed liquid
Mass ratio 1:0.5~1 adds clear water, and homogenate is made in stirring;
(2) enzymolysis processing:β-Isosorbide-5-Nitrae dextranase, cellobiohydrolase, Isosorbide-5-Nitrae-β-mannose are added in gained homogenate
Aldehydic acid lyases, bromelain, carbohydrase, 40~50 DEG C, pH 4~6, enzymolysis 7~12 hours, separation enzymolysis liquid and residual
Slag;
(3) fermentation process:In the residue obtained middle addition citrus peel residue of step (2), aspergillus niger, neurospora crassa, withered grass bar
Bacterium, 30~50 DEG C, pH5~7 are fermented 7~12 hours;
(4) prepared by fertilizer:Enzymolysis liquid obtained by step (2) is mixed with the fermentate obtained by step (3), zeolite is added
Powder, oyster shell whiting stir, and compound alga fertilizer is made in drying granulation.
In the preparation method of the compound marine alga fertilizer, step (1), brown alga, red algae, green alga, sargassum, the matter of bladder-wrack
Measure number as follows:50~70 parts of brown alga;10~15 parts of red algae;10~15 parts of green alga;5~10 parts of sargassum;Bladder-wrack 5~10
Part.
In the preparation method of the compound marine alga fertilizer, step (1), particle diameter is crushed to for 60~80 mesh.
The preparation method of the compound marine alga fertilizer, in the homogenate of step (2), the addition of enzyme is:β-Isosorbide-5-Nitrae dextranase
1.0~2.5g/L;2~2.5g/L of cellobiohydrolase;Isosorbide-5-Nitrae-β -0.8~1.2g/L of mannuronic acid lyases;Pineapple egg
White enzyme 0.5~1.0g/L, 1.5~2.0g/L of carbohydrase.
In the preparation method of the compound marine alga fertilizer, the residue of step (3), the addition of citrus peel residue for 4.0~
6.0g/kg, citrus peel residue particle diameter is 50~150 mesh;The addition of aspergillus niger is 1.0~2.0g/kg;The addition of neurospora crassa
Measure as 0.6~1.2g/kg;The addition of bacillus subtilis is 1.0~1.5g/kg.
In the preparation method of the compound marine alga fertilizer, the mixture of step (4), the addition of zeolite powder for 25~
100g/kg, the addition of oyster shell whiting is 50~200g/kg.
In the preparation method of the compound marine alga fertilizer, step (4), zeolite powder, the particle diameter of oyster shell whiting are 150~200 mesh.
In the preparation method of the compound marine alga fertilizer, step (4), drying temperature is 60~85 DEG C.
In the preparation method of the compound marine alga fertilizer, step (4), a diameter of 5~8mm is granulated.
Advantages of the present invention and beneficial effect are:
1st, the present invention is handled fresh feed by the way of cryogenic freezing is broken, effectively protects marine alga effectively to live
Property composition, make gained alga fertilizer it is nutritious.
2nd, the present invention is using mode associated with a variety of enzymes, and macromolecular carbohydrate, proteinaceous components in being homogenized to marine alga enter
Row effectively degraded, improves the extraction efficiency of DDGS in marine alga.
3rd, the present invention is suitable to the strain of agricultural production to the residue after enzymolysis by adding aspergillus niger, bacillus subtilis etc.
Carry out fermentation process.On the one hand reduce residue discharge, on the other hand residue is turned waste into wealth, ferment produce metabolite and
Thalline can be good at improving in itself the effect of fertilizer.The citrus peel residue added in fermentation process, on the one hand has
Effect alleviates fermentation ph requirement, on the other hand, substantial amounts of functional materials is contained in citrus peel residue, for example:Essential oil, orange peel
Glycosides, flavonoids etc., can further supplement the trophic component of fertilizer.
4th, the present invention carries out compounding granulation, zeolite powder, oyster shell whiting using zeolite powder, oyster shell whiting (oyster, scallop shell etc.) etc.
It is interior to contain abundant trace element (copper 96ppm, sodium 1.9wt%, magnesium 2wt%, potassium 1.38wt%, calcium 3wt%, iron
0.51wt%), while having higher cation exchange capacity (CEC) and aperture adsorptivity, nutrient element can be leaned on aperture absorption affinity and the moon
The electrostatic attraction of cation is tightly bound in around zeolite lattice, so that fertilizer efficiency is more lasting.
Brief description of the drawings
Fig. 1 is influence figure of the different fertilizer to Strawberry Leaves Determination of Chlorophyll content.
Embodiment
In specific implementation process, the preparation method of the compound marine alga fertilizer of the present invention is as follows:
(1) pre-process:Fresh brown alga (sea-tangle, sargassum etc.), red algae (seaweed, agar etc.), green alga (sea lettuce), horse hair
Algae, bladder-wrack etc. are raw material, and brown alga, red algae, green alga, sargassum, the preferred mass ratio of bladder-wrack are 7:2:11:3:5, use clear water
Clean repeatedly, remove wherein impurity;Frond is dried naturally or extrusion dehydration to water content is 40~50wt%;Frond is put
In 4~8h of freezing at -15 DEG C to -25 DEG C;60~80 mesh are crushed to using tissue mashing machine after taking-up, by feed liquid mass ratio 1:
0.5~1 addition clear water, which is mixed, is made homogenate;
(2) digest:Circumscribed β-Isosorbide-5-Nitrae-dextranase (1~2.5g/L), cellobiohydrolase are added in gained homogenate
(2~2.5g/L), Isosorbide-5-Nitrae-β-mannuronic acid lyases (0.6~1.2g/L), bromelain (0.5~1.0g/L), saccharification
Enzyme (1.5~2.0g/L), in 40~50 DEG C, the enzymolysis 7~12 hours of pH 4~6 times;After the completion of enzymolysis, using pipeline centrifuge or
Filter membrane row separation of solid and liquid, obtains marine alga enzymolysis liquid and enzymolysis residue;
(3) microbial fermentation:Citrus peel residue (4.0~6.0g/L), aspergillus niger (1.0 are added in the enzymolysis residue of gained
~2.0g/L), neurospora crassa (0.6~1.2g/L), bacillus subtilis (1.0~1.5g/L), 30~50 DEG C, pH5~
7, interval stirring is fermented 7~12 hours;
(4) prepared by fertilizer:Marine alga enzymolysis liquid obtained by step (2) is mixed with the fermentate obtained by step (3), added
Zeolite powder, the oyster shell whiting (mass ratio 1 of 150~200 mesh:0.2~0.5), granulation is dried after stirring, drying temperature control exists
60~85 DEG C, compound marine alga organic granular fertilizer is made, a diameter of 5~8mm is granulated.
Below, the present invention is further elaborated on by embodiment and accompanying drawing.
Embodiment 1
In the present embodiment, the preparation method of marine alga fertilizer is combined, following steps are specifically included:
(1) pre-process:Fresh brown alga, red algae, green alga, sargassum, bladder-wrack, brown alga, red algae, green alga, sargassum, ink
The mass ratio of angle algae is as follows:70:10:10:5:5.Clean repeatedly, filter out impurity, dehydration to water content is 45wt%, be placed in-
6h is freezed at 20 DEG C;It is 70 mesh that historrhexis's crusher machine to particle diameter is used after taking-up, by feed liquid mass ratio 1:0.8 adds clear water, stirs
Mixing is into homogenate;
(2) enzymolysis processing:β-Isosorbide-5-Nitrae dextranase, cellobiohydrolase, Isosorbide-5-Nitrae-β-mannose are added in gained homogenate
Aldehydic acid lyases, bromelain, carbohydrase, 45 DEG C, pH 5, enzymolysis 10 hours, separation enzymolysis liquid and residue;
Wherein, the addition of enzyme is:β-Isosorbide-5-Nitrae dextranase 1.5g/L;Cellobiohydrolase 2.2g/L;Isosorbide-5-Nitrae-β-sweet dew
Uronic acid lyases 1.0g/L;Bromelain 0.8g/L, carbohydrase 1.8g/L.
(3) fermentation process:In the residue obtained middle addition citrus peel residue of step (2), aspergillus niger, neurospora crassa, withered grass bar
Bacterium, 40 DEG C, pH6 ferment 9 hours;
Wherein, the addition of citrus peel residue is 5.0g/kg, and citrus peel residue particle diameter is 100 mesh;The addition of aspergillus niger is
1.5g/kg;The addition of neurospora crassa is 0.9g/kg;The addition of bacillus subtilis is 1.2g/kg.
(4) prepared by fertilizer:Enzymolysis liquid obtained by step (2) is mixed with the fermentate obtained by step (3), zeolite is added
Powder, oyster shell whiting stir, and zeolite powder, the particle diameter of oyster shell whiting are 180 mesh, and zeolite powder, the addition of oyster shell whiting are respectively:40g/
Kg, 160g/kg, at 70 DEG C of drying temperature, compound alga fertilizer is made in granulation, granulates a diameter of 6mm.
Embodiment 2
In the present embodiment, the preparation method of marine alga fertilizer is combined, following steps are specifically included:
(1) pre-process:Fresh brown alga, red algae, green alga, sargassum, bladder-wrack, brown alga, red algae, green alga, sargassum, ink
The mass ratio of angle algae is as follows:50:15:15:10:10.Clean repeatedly, filter out impurity, dehydration to water content is 41wt%, is put
In freezing 5h at -16 DEG C;It is 60 mesh that historrhexis's crusher machine to particle diameter is used after taking-up, by feed liquid mass ratio 1:0.6 adds clear water,
Homogenate is made in stirring;
(2) enzymolysis processing:β-Isosorbide-5-Nitrae dextranase, cellobiohydrolase, Isosorbide-5-Nitrae-β-mannose are added in gained homogenate
Aldehydic acid lyases, bromelain, carbohydrase, 42 DEG C, pH 4, enzymolysis 8 hours, separation enzymolysis liquid and residue;
Wherein, the addition of enzyme is:β-Isosorbide-5-Nitrae dextranase 1.2g/L;Cellobiohydrolase 2.1g/L;Isosorbide-5-Nitrae-β-sweet dew
Uronic acid lyases 0.9g/L;Bromelain 0.6g/L, carbohydrase 1.6g/L.
(3) fermentation process:In the residue obtained middle addition citrus peel residue of step (2), aspergillus niger, neurospora crassa, withered grass bar
Bacterium, 32 DEG C, pH5 ferment 8 hours;
Wherein, the addition of citrus peel residue is 4.2g/kg, and citrus peel residue particle diameter is 60 mesh;The addition of aspergillus niger is
1.2g/kg;The addition of neurospora crassa is 0.7g/kg;The addition of bacillus subtilis is 1.2g/kg.
(4) prepared by fertilizer:Enzymolysis liquid obtained by step (2) is mixed with the fermentate obtained by step (3), zeolite is added
Powder, oyster shell whiting stir, and zeolite powder, the particle diameter of oyster shell whiting are 150 mesh, and zeolite powder, the addition of oyster shell whiting are respectively:60g/
Kg, 120g/kg, at 65 DEG C of drying temperature, compound alga fertilizer is made in granulation, granulates a diameter of 5mm.
Embodiment 3
In the present embodiment, the preparation method of marine alga fertilizer is combined, following steps are specifically included:
(1) pre-process:Fresh brown alga, red algae, green alga, sargassum, bladder-wrack, brown alga, red algae, green alga, sargassum, ink
The mass ratio of angle algae is as follows:65:10:11:6:8.Clean repeatedly, filter out impurity, dehydration to water content is 48wt%, be placed in-
7h is freezed at 22 DEG C;It is 80 mesh that historrhexis's crusher machine to particle diameter is used after taking-up, by feed liquid mass ratio 1:0.9 adds clear water, stirs
Mixing is into homogenate;
(2) enzymolysis processing:β-Isosorbide-5-Nitrae dextranase, cellobiohydrolase, Isosorbide-5-Nitrae-β-mannose are added in gained homogenate
Aldehydic acid lyases, bromelain, carbohydrase, 48 DEG C, pH 6, enzymolysis 10 hours, separation enzymolysis liquid and residue;
Wherein, the addition of enzyme is:β-Isosorbide-5-Nitrae dextranase 2.4g/L;Cellobiohydrolase 2.4g/L;Isosorbide-5-Nitrae-β-sweet dew
Uronic acid lyases 1.1g/L;Bromelain 0.9g/L, carbohydrase 1.9g/L.
(3) fermentation process:In the residue obtained middle addition citrus peel residue of step (2), aspergillus niger, neurospora crassa, withered grass bar
Bacterium, 50 DEG C, pH7 ferment 11 hours;
Wherein, the addition of citrus peel residue is 5.8g/kg, and citrus peel residue particle diameter is 150 mesh;The addition of aspergillus niger is
1.8g/kg;The addition of neurospora crassa is 1.1g/kg;The addition of bacillus subtilis is 1.4g/kg.
(4) prepared by fertilizer:Enzymolysis liquid obtained by step (2) is mixed with the fermentate obtained by step (3), zeolite is added
Powder, oyster shell whiting stir, and zeolite powder, the particle diameter of oyster shell whiting are 200 mesh, and zeolite powder, the addition of oyster shell whiting are respectively:30g/
Kg, 100g/kg, in the case where drying temperature is 80 DEG C, compound alga fertilizer is made in granulation, granulates a diameter of 7mm.
Application examples:Influence of the compound fertilizer to Strawberry Leaves Determination of Chlorophyll content
Phjytotron is set as 26 DEG C, humidity 55%RH.Using identical flowerpot and soil, same plant height is selected, is not hung
The strawberry of fruit is tested, and by fixed dose (100ml and 500ml, less than 500ml clear water polishings), is existed weekly
The root of strawberry sprays the compound marine alga fertilizer of different dilution ratios, and negative control group sprays the clear water of same volume.With market
The Rayleigh fertilizer purchased is as positive control, and the test period is 2 months.15 groups of progress of experiment point, every group of 10 plants of strawberries.Off-test
Afterwards, each test group blade is plucked, is homogenized after mixing, utilizes chlorophyll test kit pilot blade Determination of Chlorophyll content.
As a result as shown in figure 1, compared with the control, applying mixing quality ratio 20:1~60:The strawberry of 1 compound alga fertilizer is planted
Strain, blade Determination of Chlorophyll concentration is in 34.315mg/L~38.722mg/L, obviously higher than clear water control group, is also higher than simple
Big algae fertilizer (sea-tangle fertilizer), microalgae fertilizer, Rayleigh fertilizer, illustrate that the compound alga fertilizer in this dilution factor contains to Strawberry Leaves Determination of Chlorophyll
The lifting of amount has preferable effect.Dose contrast display, 100ml and 500ml dose influence are little.Applied for the later stage
Consider, can using 100ml dose undoubtedly more economical and effective.
Claims (9)
1. a kind of preparation method of compound marine alga fertilizer, it is characterised in that specifically include following steps:
(1) pre-process:Fresh brown alga, red algae, green alga, sargassum, bladder-wrack, clean repeatedly, filter out impurity, and dehydration is to aqueous
Measure as 40~50wt%, be placed in 4~8h of freezing at -15 DEG C to -25 DEG C;Historrhexis's crusher machine is used after taking-up, by feed liquid quality
Than 1:0.5~1 adds clear water, and homogenate is made in stirring;
(2) enzymolysis processing:β-Isosorbide-5-Nitrae dextranase, cellobiohydrolase, Isosorbide-5-Nitrae-β-mannuronic acid are added in gained homogenate
Lyases, bromelain, carbohydrase, 40~50 DEG C, pH 4~6, enzymolysis 7~12 hours, separation enzymolysis liquid and residue;
(3) fermentation process:In the residue obtained middle addition citrus peel residue of step (2), aspergillus niger, neurospora crassa, hay bacillus, 30
~50 DEG C, pH5~7, ferment 7~12 hours;
(4) prepared by fertilizer:Enzymolysis liquid obtained by step (2) is mixed with the fermentate obtained by step (3), zeolite powder, shellfish is added
Shell powder stirs, and compound alga fertilizer is made in drying granulation.
2. according to claim 1 be combined marine alga fertilizer preparation method, it is characterised in that in step (1), brown alga, red algae,
Green alga, sargassum, bladder-wrack mass fraction it is as follows:50~70 parts of brown alga;10~15 parts of red algae;10~15 parts of green alga;Horse hair
5~10 parts of algae;5~10 parts of bladder-wrack.
3. the preparation method of marine alga fertilizer is combined according to claim 1, it is characterised in that in step (1), be crushed to particle diameter
For 60~80 mesh.
4. the preparation method of marine alga fertilizer is combined according to claim 1, it is characterised in that in the homogenate of step (2), enzyme
Addition is:β -1.0~2.5g/L of Isosorbide-5-Nitrae dextranase;2~2.5g/L of cellobiohydrolase;Isosorbide-5-Nitrae-β-mannuronic acid splits
Solve 0.8~1.2g/L of enzyme;0.5~1.0g/L of bromelain, 1.5~2.0g/L of carbohydrase.
5. the preparation method of marine alga fertilizer is combined according to claim 1, it is characterised in that in the residue of step (3), citrus
The addition of skin slag is 4.0~6.0g/kg, and citrus peel residue particle diameter is 50~150 mesh;The addition of aspergillus niger is 1.0~2.0g/
kg;The addition of neurospora crassa is 0.6~1.2g/kg;The addition of bacillus subtilis is 1.0~1.5g/kg.
6. the preparation method of marine alga fertilizer is combined according to claim 1, it is characterised in that in the mixture of step (4), boiling
The addition of stone flour is 25~100g/kg, and the addition of oyster shell whiting is 50~200g/kg.
7. the preparation method of marine alga fertilizer is combined according to claim 1 or 6, it is characterised in that in step (4), zeolite powder,
The particle diameter of oyster shell whiting is 150~200 mesh.
8. the preparation method of marine alga fertilizer is combined according to claim 1, it is characterised in that in step (4), drying temperature is
60~85 DEG C.
9. being combined the preparation method of marine alga fertilizer according to claim 1, it is characterised in that in step (4), granulate a diameter of
5~8mm.
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CN112794767A (en) * | 2021-02-25 | 2021-05-14 | 湖南开门子肥业有限公司 | Compound seaweed fertilizer and preparation method thereof |
CN114164240A (en) * | 2021-09-08 | 2022-03-11 | 新洋丰农业科技股份有限公司 | Preparation method of seaweed extract by segmented enzymolysis |
CN114504065A (en) * | 2022-03-09 | 2022-05-17 | 湖北丰甜生物科技有限公司 | Method for producing natural bait low-temperature composite algae for aquatic animals |
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