CN106927941A - A kind of organic silicon fertilizer and preparation method thereof - Google Patents
A kind of organic silicon fertilizer and preparation method thereof Download PDFInfo
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- CN106927941A CN106927941A CN201511030420.5A CN201511030420A CN106927941A CN 106927941 A CN106927941 A CN 106927941A CN 201511030420 A CN201511030420 A CN 201511030420A CN 106927941 A CN106927941 A CN 106927941A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a kind of organic silicon fertilizer and preparation method thereof, described method includes step：By silicate bacteria, black-koji mould, bacillus licheniformis, bacillus subtilis, Lactococcus lactis, Lactobacillus plantarum, alcohol yeast according to viable count 30~35：25~30：8~10：8~10：8~10：8~10：13~15 ratio hybrid anaerobic fermentation, is made the zymocyte liquid of pH value 4.0~3.5；Sterilized potassium feldspar is mixed with zymocyte liquid, benayl aminopurine is selectively added, in 28~37 DEG C of closed standing for fermentation more than 20 days, products therefrom was organic silicon fertilizer.The present invention prepares biological organic siliceous fertilizer series of products by with potassium feldspar as raw material, using microorganism zymolysis technique, and gained siliceous fertilizer product stability is good, crop absorption ratio is high, practical.
Description
Technical field
The present invention is that specifically, the present invention is on one kind with biology enzyme on a kind of organic silicon fertilizer and preparation method thereof
Solution technology prepares the method and preparation-obtained organic silicon fertilizer of organic silicon fertilizer, belongs to novel biological fertilizer technical field.
Background technology
Element silicon proposes more late than nitrogen, phosphorus, potassium as the concept of fertilizer.Nineteen twenty-six, the U.S. found that element silicon can make water first
Rice, sugarcane production-increasing；Nineteen fifty-one Yamanashi, Japan university too Tian Daoxiong and holt are taught and find and propose paddy rice " autumn falls ", rice blast
Disease, early ageing and rotten etc. are relevant with scarce element silicon.Nineteen fifty-three have developed in the world first produced using industrial residue
New-type fertilizer --- siliceous fertilizer, nineteen fifty-five, siliceous fertilizer was written with fertilizer law by Japan.Then, South Korea, Korea, Philippine, Thailand and
TaiWan, China etc. has successively introduced the siliceous fertilizer production technology of Japan, enables siliceous fertilizer in the large-scale promotion application seventies.The U.S.,
Mauritius etc. absorbs the technical experience of Japan, sugarcane yield has almost been turned over some.Siliceous fertilizer Xian Bei worlds soil educational circles is true
Think after nitrogen (N), phosphorus (P2O5), potassium (K2O the fourth-largest element fertilizer after).13rd International Plant of September Japan in 1997
In NI, U.S., day, in many achievements in research show 21 century silicon element as plant must nutrient possibility.But
Japan have developed flyash-Si, potassic fertilizer again in recent years, both solve the problems, such as flyash, and the loss that potash fertilizer is solved again is asked
Topic.Autumn in 1999, the scientific seminar of " silicon is agriculturally " for the first time, mark are held in the world in the Florida State in the U.S.
Silicon nutrition and caused global concern, also indicate that the climax of popularization and application siliceous fertilizer in the world has arrived.China also
Silicon fertilizer is formally classified as one of agricultural fertilizer, and silicon fertilizer industry tentative standard has been promulgated with 2004.
Element silicon has significant impact to the yield and quality of various crop.Research shows：Element silicon is a kind of to paddy rice
Typical beneficial element, silicone content has exceeded the summation of the elements such as nitrogen, phosphorus, potassium in rice plant.To be inhaled during Rice Production
Substantial amounts of silicon is received, the silicon for having 20%~50% is provided by irrigation water, 75%~80% silicon comes from soil.With per mu yield paddy
500kg is calculated, and its stalk and paddy absorb silicon (SiO2) up to 75kg/ mus of amount, than N, P for absorbing2O5、K2O three's summation is higher by
1.5 times.Silicon is the chief component in plant.Applying silicon fertilizer can improve Rice Photosynthesis efficiency, improve root system and live
Power, increases surface texture intensity, makes stiff in straw, resistant to lodging, reduces pest and disease damage.Japan is annual with Korea merely with melting waste slag
Just production siliceous fertilizer (slag silicate calcium) 100~1,700,000 t, is mainly used in paddy rice.
In addition to paddy rice, sugarcane, silicon fertilizer has to crops such as wheat, corn, water fruits and vegetables, peanut, cotton, tobacco
Different degrees of effect of increasing production.Japanese high bridge English one have studied 175 kinds of plants, wherein：Thermophilic silicon plant has 42 kinds, accounts for 24%.It is right
The thermophilic silicon plant such as grass family, Cyperaceae, silicon is necessary element, and to non-thermophilic silicon plant such as tomato, cucumber etc., scarce silicon is susceptible to suffer from physiology
Disease, silicon is beneficial element.
Siliceous fertilizer species in the market is a lot, for example, Liaoning research institute of Academy of Agricultural Sciences with industrial residue, flyash and
Nitrogen phosphorus fertilizer is the silicon compound nitrogen phosphate fertilizer that raw material is developed；The SiO of the emerging super fine powder factory production of Tonghua City hundred2>=36.0%, CaO >=
20.0%th, calcareous fertilisers of MgO >=6.0%, potassium oxide >=0.6% etc..Separately have been reported that the slag of smeltery can also make siliceous fertilizer.
CN1962564A discloses a kind of production technology of organic silicon fertilizer, wherein using silica, aluminum oxide, three kinds of materials of calcium oxide
As raw material, reacted with water, reactant after it dry, pulverize with feces of livestock and poultry mixed fermentation, be made organic silicon fertilizer.
CN101096326A discloses a kind of organic silicon leaf fertilizer and preparation method thereof, wherein extract organosilicon from rice husk, and with nitrogen,
The compoundings such as phosphorus, potash fertilizer, micro- fertilizer, humic acid, plant growth regulator prepare foliar fertilizer.CN101768023A discloses a kind of organic
Silicon fertilizer, the material composition and its percentage by weight of the obtained organic agricultural fertilizer are：Oil shale powder raw material：60%~
70%, calcium superphosphate：10%~15%, potassium sulfate：10%~15%, magnesium sulfate：5%~10%.CN103539512A is disclosed
A kind of humic acid 30%~60%, the paddy rice-dedicated fertilizer of SILICA FUME 40%~70%.CN102875212A disclose it is a kind of with
Alkaline residue mixing various plants residuum, potassium nitrate, calcium superphosphate and animal wastes etc., under being placed in sunshine condition prepared by composting decomposition
The organic silicon fertilizer for obtaining.
The content of the invention
It is an object of the present invention to provide the preparation method of organic silicon fertilizer, to prepare a kind of crop absorption rate
High, high-quality and efficient comprehensive silicon bio-bacterial manure.
Another object of the present invention is to provide a kind of crop absorption rate organic silicon fertilizer high, high-quality and efficient.
Another object of the present invention is to provide a kind of mixed bacteria liquid for preparing the organic silicon fertilizer.
In technology of the invention, microorganism is mainly used to digest element silicon technology, having prepared a kind of crop can be directly
Compound bio organic silicon fertilizer series of products absorb, high-quality and efficient.
Specifically, on the one hand, the invention provides a kind of preparation method of organic silicon fertilizer, the method comprising the steps of：
A, by silicate bacteria (Bacillus mucilaginosus), black-koji mould (Aspergillus niger),
Clothing bacillus (Bacillus licheniformis), bacillus subtilis (Bacillus subtilis), Lactococcus lactis
(Lactoccus llctis subsplactjs), Lactobacillus plantarum (Lactobacillut plantarum Holland), wine
Smart saccharomycete (Saccharomyces cerevisiae) is according to viable count 30~35：25~30：8~10：8~10：8~10：8
~10：13~15 ratio mixing, in being fermented more than 15 days under 28~37 DEG C of anaerobic conditions, is made the fermentation of pH value 4.0~3.5
Bacterium solution；
B, potassium feldspar is mixed with the zymocyte liquid of step a, the two mixed proportion：Zymocyte liquid weight and potassium feldspar weight
Measure is 2~6：1；
C, by the mixed system of step b in 28~37 DEG C of closed standing for fermentation more than 20 days, organic silicon fertilizer is obtained.
Specific embodiment of the invention, the preparation method of organic silicon fertilizer of the invention is described in the step a
Silicate bacteria (Bacillus mucilaginosus), bacillus licheniformis (Bacillus licheniformis), withered grass
Bacillus (Bacillus subtilis) is the conventional strain in microbial organic fertilizer field, Lactococcus lactis (Lactoccus
Llctis subsplactjs), Lactobacillus plantarum (Lactobacillut plantarum Holland), alcohol yeast
(Saccharomyces cerevisiae, i.e. saccharomyces cerevisiae) is the conventional strain of field of food fermentation, black-koji mould
(Aspergillus niger) produces amylase, protease, carbohydrase, the common food of pectic acid using soy sauce, vinegar food factory
Level aspergillus niger.Additionally, wherein also optionally further include yellow ocher streptomycete (Streptomyces silaceus),
One or more in photosynthetic bacteria, bafillus natto, B. mucilaginocus etc., mixed proportion of these strains in mixed bacteria liquid can divide
It is not：One or more in silicate bacteria and yellow ocher streptomycete, photosynthetic bacteria, bafillus natto, B. mucilaginocus etc. of work
The ratio between bacterium number is 30~35：1~10.Each strain of the invention can be commercially available from Chinese agriculture microbial bacteria guarantor center, this
Metabolism producing enzyme performance during multi-cultur es culture, to specific strain characteristic without particular requirement, usual example are mainly used in invention
Each bacterial strain of deposit number Accc10012, Accc11080, Accc11025, Accc40022, Accc11016 can such as be chosen.This
A little strains are the conventional strains of art, can respectively be activated according to the record of prior art or the suggestion of strain supply businessman
Culture, is prepared into seed liquor, and hybrid anaerobic fermentation is carried out according still further to described viable count ratio.
In the present invention, the acidophilus of category more than above strain is heat-resisting, reproductive capacity is strong, the metabolism change that frequency is fast, inulinase-producing activity is high can be certainly
The type of supporting microorganism.The largely competent cell body rich in various biology enzymes is obtained by (aerobic) fermentation of individual plant, then by different strain
The enzyme system of generation is different, and anaerobic fermentation is stood after being combined by different proportion, and domestication culture promotes cell fusion.Fermentation 15 days with
On, after typically fermenting 15~20 days, mixed system pH value 4.0~3.5, that is, obtain the various lifes of stability of the invention after testing
The compound secondary metabolism energy of thing enzyme, makees the seed liquor of enzymolysis agent.
Microorganism is that indirectly, the complex effect of various biology enzymes is to enzymolysis potassium feldspar to the effect that ore deposit element is catalytically decomposed
There is very strong affinity and selectivity.Experiment proves that silicate bacteria can be in nitrogen-free agars such as potassium feldspar, apatite, ground phosphate rock
Upper growth, expands fermentation and produces certain organic acid, amino acid and a large amount of biotins；Black-koji mould grows preference temperature at 37 DEG C
Produce acid protease, cellulase, carbohydrase；Bacillus licheniformis and bacillus subtilis breeding life power are extremely strong, lichens bud
Spore bacillus can in stone seam growth and breeding, once temperature condition can suitably make stone mountain be covered with moss；Bacillus subtilis, hair
24 hours viable counts of ferment culture are up to：4.21×1010cfu/ml.Using the biochemistry advantage composite fermentation of multi-cultur es, producing enzyme passage
Property it is good, enzymatic reaction speed accelerate, product is changed into by zymolyte also higher.
In the present invention, culture medium during each Spawn incubation can also refer to the record of prior art or building for strain supply businessman
View.Growth of microorganism needs nutrient, conventional mainly inorganic salts：Phosphorus, calcium, magnesium, sulphur+carbon source, nitrogen source.As silicate is thin
The typical culture medium each component ratio of bacterium：K2HPO40.5g、CaCO31g、MgSO40.2g+ mannitol 10g, yeast extract 0.4g, water
1000ml；The typical culture medium each component ratio of black-koji mould：PDA- (czapek's medium)；Lichens, bacillus subtilis typical case
Culture medium each component ratio：Beef extract, peptone, NaCl each 5g, water 1000ml etc..
Specific embodiment of the invention, in the preparation method of organic silicon fertilizer of the invention, the independent liquid of each strain
The composite anaerobic fermentation of body culture and multi-cultur es can also be with the soya-bean milk of solid content 8~20% as culture medium, or in soya-bean milk
In further addition peptone, yeast extract or trace element meeting the nutritional need of growth of microorganism.
Specific embodiment of the invention, the preparation method of organic silicon fertilizer of the invention, wherein, potassium is long in step b
Stone is the stone or stone flour to granularity less than 5cm × 5cm × 5cm size-reduced in advance.
Specific embodiment of the invention, the preparation method of organic silicon fertilizer of the invention, potassium feldspar need not in step b
Be added to by being sterilized in fermentation system of the invention, also can in advance by sterilizing, for example, the sterilizing of potassium feldspar be according to
What following methods were carried out：
Potassium feldspar clear water is washed into floating dust thing, spraying surface disinfection with 75% ethanol is placed in closed environment
Closing more than 8 hours, taking out standing makes ethanol volatilize, the potassium feldspar for being sterilized.
Specific embodiment of the invention, in the preparation method of organic silicon fertilizer of the invention, the mixed system of step b
Middle alternative addition benayl aminopurine, addition of the benayl aminopurine in zymocyte liquid is preferably more than 20mg/L zymophytes
Liquid.
Specific embodiment of the invention, the preparation method of organic silicon fertilizer of the invention, wherein, step c and step b
After mixing sealed fermenting was more than 20 days, fermentation system produces layering, and upper strata is suspension, centre is colloidal thing, bottom is heavy
Starch.Wherein, upper strata suspension section be silicone fluid (dark brown), living bacteria count (cfu) >=200,000,000/g, coliform group count≤
100/g, induced worm egg death rate >=95%, heavy metal As, Ca, Pb, Cr, Hg content's index meets the rule in NY/T798-2004
It is fixed.The colloidal thing in middle level be colloidal silica (brown color goo), living bacteria count (cfu) >=0.5 hundred million/g, coliform group count≤
100/g, induced worm egg death rate >=95%, heavy metal content index meets the regulation of NY/T798-2004.The precipitation portion of bottom
It is divided into silicon element slag (canescence), living bacteria count (cfu) >=0.2 hundred million/g, coliform group count≤100/g, induced worm egg death rate
>=95%, heavy metal content index meets NY/884-2012 regulations.This three parts (upper strata suspension, intermediate rubber of fermentation system
Body shape thing, bottom sediment thing) can be respectively as biological organic siliceous fertilizer, or further mixing unclassified stores prepares organosilicon
Fertilizer.Therefore, the preparation method of organic silicon fertilizer of the invention includes step：
D, by the upper strata suspension of fermentation system, mesocolloid shape thing, bottom sediment thing any one or more separation,
Respectively as organic silicon fertilizer or mixing two or three preparation organic silicon fertilizer therein, or with one or more therein more
Further organic silicon fertilizer product is mixed with other raw materials.
Specific embodiment of the invention, the preparation method of organic silicon fertilizer of the invention, it may include below at least one
Individual step：
D-1, the upper strata suspension separation by fermentation system, prepare liquid silicon fertilizer；
D-2, the mesocolloid shape thing separation by fermentation system, prepare pulvis siliceous fertilizer；
D-3, the bottom sediment thing separation by fermentation system, prepare particle siliceous fertilizer.
When more specifically preparing liquid silicon fertilizer, carry out in accordance with the following methods：
Directly it is separated by filtration the upper strata suspension stoste as liquid silicon fertilizer that the fermentation system is produced；
Or by 3~8 parts of humic acid, 90~95 parts of the upper strata suspension of 0~8 part of potassium dihydrogen phosphate and the fermentation system
Mixing, the liquid of 18~35 DEG C of standing for fermentation after 12~72 hours is liquid silicon fertilizer.
When preparing pulvis siliceous fertilizer, can carry out in accordance with the following methods：
By 10~30 parts of flyash, 10~30 parts of humic acid, 0~6 part of potassium dihydrogen phosphate, trace element 0~3 part with it is described
35~80 parts of mixing of mesocolloid shape thing of fermentation system, are prepared into pulvis siliceous fertilizer；
Preferably, with flyash 20%, the mesocolloid shape thing 60% that humic acid 20% is carrier and the fermentation system
Mixing, is prepared into pulvis siliceous fertilizer.
When preparing particle siliceous fertilizer, carry out in accordance with the following methods：
20~30 parts of humic acid, careless 20~30 parts of carbon, 5~15 parts of wormcast, 3~6 parts of clay, 0~8 part of trace element with
15~40 parts of the bottom sediment thing mixing of the fermentation system, directly under granulation or airtight condition 18~35 DEG C ferment 5~10
Granulated after it, obtain particle siliceous fertilizer；
Preferably, with humic acid 30%, careless carbon 30%, the wormcast 15% for becoming thoroughly decomposed, clay 5% for carrier and the fermentation
The bottom sediment thing 20% of system mixes, and directly granulation obtains particle siliceous fertilizer.
On the other hand, present invention also offers a kind of organic silicon fertilizer, it is prepared according to the above-mentioned method of the present invention
's.As described above, organic silicon fertilizer of the invention, can be by 28~37 DEG C of closed standing for fermentation 20~40 preferably 30 in step c
The tunning of~40 days is directly as organic silicon fertilizer；Can also be by sealed fermenting in step c more than 20 days after typically 30
Any one or more separation in upper strata suspension, mesocolloid shape thing, the bottom sediment thing of~40 days or so, respectively as having
Machine siliceous fertilizer mixes two or three preparation organic silicon fertilizer therein；Can also be with sealed fermenting in step c more than 20 days after
One or more in upper strata suspension, mesocolloid shape thing, the bottom sediment thing of typically 30~40 days or so is further
Organic silicon fertilizer product is mixed with other raw materials, more specifically can be that the upper strata suspension of fermentation system is separated into mixing
Other raw materials prepare liquid silicon fertilizer, or the mesocolloid shape thing separation of fermentation system are mixed into other raw materials and prepare pulvis
Siliceous fertilizer, can also be that the bottom sediment thing separation of fermentation system is mixed into other raw materials prepares particle siliceous fertilizer.
Additionally, present invention also offers a kind of zymocyte liquid for preparing described organic silicon fertilizer, it is according to following
What method was prepared：By silicate bacteria (Bacillus mucilaginosus), black-koji mould (Aspergillus
Niger), bacillus licheniformis (Bacillus licheniformis), bacillus subtilis (Bacillus subtilis),
Lactococcus lactis (Lactoccus llctis subsplactjs), Lactobacillus plantarum (Lactobacillut plantarum
Holland), alcohol yeast (Saccharomyces cerevisiae) is according to viable count 30~35：25~30：8~10：8
~10：8~10：8~10：13~15 ratio mixing, in being fermented more than 15 days under 28~37 DEG C of anaerobic conditions, is made pH value
4.0~3.5 zymocyte liquid.The zymocyte liquid contains the high active substance of specific enzymolysis element silicon, is a kind of biological enzymolysis liquid.
In sum, the present invention promotes the Efficient Conversion of biomass to digest core technology as breach, realizes biology
The essence for digesting mineral matter technology breaks through.The present invention by with potassium feldspar as raw material, using biological enzymolysis element silicon technology, can
Prepare the organic silicon fertilizer of the features such as effective ingredient good stability, crop absorption ratio are high, practical, water-soluble composition is more.It is real
Test and show, the organic silicon fertilizer is applied to the multiple kinds of crops such as paddy rice, wheat, corn, sugarcane, tobacco, peanut, fruit tree, can lure
The division of crop seed eukaryotic is led, the biosynthesis ability of make to take root cell and protein strengthens, in Seed Germination,
Taking root increases, thus development of plants children phase can just fully absorb the moisture and nutritional ingredient in soil, be the later stage of crop
Material base is established in growth；Promote photosynthesis enhancing, strengthen Chlorophyll synthesis ability, carbon aquation is constantly formed by blade
Compound existence deposit nutrient, and it is eventually fed the fruit of plant.
Organic silicon fertilizer product of the invention is a kind of activation repairing soil, the synergist of fortification of plants cytotrophy, is it
A kind of incomparable Multifunction fertilizer of its chemical fertilizer.Both fertilizer can have been made nutrient was provided, soil conditioner had been can be used as again
Improved soil, while having the effect of diseases prevention, insect prevention and degraded toxin and heavy metal pollution concurrently.So that its is nonpoisonous and tasteless, never degenerate, no
The outstanding feature such as loss, nuisanceless, the exclusive invention of category, determining will be as the high-efficiency high-quality fertilizer of development ecological organic agricultural.
Specific embodiment
In order to be more clearly understood to technical characteristic of the invention, purpose and beneficial effect, in conjunction with instantiation
Technical scheme is carried out described further below, techniques not described means of the present invention are entered by usual manner in the art
OK, it should be understood that these examples are only illustrative of the invention and is not intended to limit the scope of the invention.
Embodiment 1
First, the preparation of biological enzymolysis liquid
1st, the selection of strain
A, silicate bacteria (Bacillus mucilaginosus)；
B, black-koji mould (Aspergillus niger)；
C, bacillus licheniformis (Bacillus licheniformis)；
D, bacillus subtilis (Bacillus subtilis)；
E, Lactococcus lactis (Lactoccus llctis subsp lactjs)；
F, Lactobacillus plantarum (Lactobacillut plantarum Holland)；
G, alcohol yeast (Saccharomyces cerevisiae).
Each strain is purchased from Chinese agriculture microbial bacteria and protects center.
2nd, individual plant aerobic fermentation, the suggestion of culture medium and fermentation condition reference strain supplier are carried out above-mentioned strain.Respectively
Strain can ferment to viable count and reach 5~1,000,000,000/gram or 5~1,000,000,000/mL concentrations above, as seed liquor.Afterwards by each seed
Liquid is according to silicate bacteria (Bacillus mucilaginosus), black-koji mould (Aspergillus niger), lichens gemma
Bacillus (Bacillus licheniformis), bacillus subtilis (Bacillus subtilis), Lactococcus lactis
(Lactoccus llctis subsplactjs), Lactobacillus plantarum (Lactobacillut plantarum Holland), wine
Smart saccharomycete (Saccharomyces cerevisiae) viable count about 35：30：10：10：10：10：15 ratio mixing, with solid
The soybean milk of content 10% is issued as nutrient solution, inoculation mixed bacteria liquid (inoculum concentration about 5%) in 28~30 DEG C of anaerobic conditions
Ferment, ferments about 20 days, obtains the acid fermentation bacterium solution of pH value about 3.8, wherein having rich in various biology enzymes, to enzymolysis element silicon
Certain selectivity, is that high-activity biological digests liquid (stand-by).
2nd, potassium feldspar treatment
1、K2O·Al2O3·6SiO2Molecular formula, main component is：SiO2、Al2O3、K2O、Na2O, CaO, are alkaline-earth metal
Aluminium silicate mineral matter crystal structure, belong to rack-like structure.
The 2nd, feldspar in powder is broken to the bulk of no more than 5cm × 5cm × 5cm, floating dust thing is washed with clear water, use 75% second
Alcohol sprinkling surface disinfection is placed in closed container, is closed more than 8 hours, is taken out and (allow ethanol to wave naturally after standing 2 hours
Hair), the potassium feldspar for being sterilized is put into vertical response container (vitreum is optimal).
3rd, enzymolysis process
1st, the potassium feldspar after sterilizing is placed in vertical type fermentation container, enzymolysis liquid is slowly added into proportion, ratio is 5：1 or
4：1 (metering is carried out suitably adjusting by the difference of potassium feldspar silicon content).
2nd, 28~30 DEG C of sealing and fermentings stand catalytic decomposition, and potassium feldspar can be observed within the 10th~15 day has outstanding in a liquid
Floating, 15~20 days potassium feldspar leachables are presented splashing type bubble, and potassium feldspar starts cracking, and silica gel suspension progressively immerses bacteria liquid
In, pH value is incrementally increased.
3rd, by standing, enzymatic reaction system double decomposition is settled 7~10 days, and the upper suspension of generation is by fermentation system layering
Silicone fluid, intermediate complex is colloidal silica, and bottom sediment thing is silicon element slag.
4th, the preparation of liquid silicon fertilizer
The upper suspension (composite bacteria liquid) for leaching enzymatic hydrolysis and fermentation generation is liquid silicon fertilizer.
After testing, the supernatant liquid part that above-mentioned fermentation system is produced is silicone fluid (dark brown) to main component, and pH value is
11 (alkalescence), living bacteria count (cfu) >=200,000,000/g, coliform group count≤100/g, induced worm egg death rate >=95%, heavy metal
As, Ca, Pb, Cr, Hg content's index meet NY/T798-2004 regulations.
Products characteristics：This product can be completely soluble complex biological fluid silicon fertilizer, and its water solubility is titanium dioxide
More than ten thousand times of silicon, are the silicon nutrients that uniquely can be directly absorbed by plant.Fundamentally solve activity, the water of element silicon
Dissolubility and the difficult problem of solubility in citric acid, are the single-element fertilizer with national standard.Its main feature：
(1) suitable foliage spray, irrigations, rush apply, foliage top dressing.Nutrient Absorption is fast, and fertilizer efficiency is good, and enzymolysis silicon nutrient is in ion
State, crop can directly absorb.By the activity of microorganism, nutrient to the transportation range of plant, utilization rate are shortened
Up to 100%.
(2) present invention is silicon, potassium complex biological fertilizer, can meet paddy rice, wheat, sugarcane, corn and happiness silicon, the fruit of happiness potassium
The crop particularity such as tree, vegetables needs fertilizer, the demand of nutrient can be determined to support according to soil nutrient Condition of abundance or deficiency and crop
The proportioning divided, determines dose and sprays number of times, nontoxic.
(3) present invention is with liquid silicic acid potassium and middle trace element, the compound system of organic substance equilibrium establishment, scientific matching
Fertilizer efficiency is favorably improved to the utilization rate of element silicon and various nutrients using crop can be effectively improved, is for spraying and filling
Fertilising is irrigate, the new-type fertilizer in water-fertilizer integral new agricultural technology field is realized.
The compound bacteria liquid fertilizer 1g of the present embodiment is sprayed onto the quartz sand soaked by Dobereiner nitrogen-free agars
Upper (putting quartz sand 25g per ware), determines by 5,10,15 nitrogen quantity fixed round the clock, and using individually fix bacterium bacterium solution as than
Compared with, not spray the sample of any bacterium solution as control.Result is referring to table 1 below.
Table 1
The composite bacteria liquid of the present embodiment and the only culture of phosphobacteria containing gemma are inoculated in the soil of sterilizing respectively, are passed through
15 culture round the clock, determines the situation of change of phosphobacteria vegetative cell and Number of spores, knot on gravy peptone agar plate
Fruit is recorded in table 2.Non-sterilized soil is separately taken, composite bacteria liquid and single phosphobacteria is respectively connected to, not connect bacterium as control,
Thermophilic culture 10 days, determines each soil water soluble phosphorus content, is as a result recorded in table 3.
Table 2
Table 3
※ improves 183.33% and improves 30.8% than independent phosphobacteria than control
5th, the preparation of pulvis siliceous fertilizer
The middle level complex compound that above-mentioned fermentation system is produced is colloidal silica (brown color goo), is containing effective silicon composition
38%, pH value about 9.5 (alkalescence), living bacteria count (cfu) >=0.5 hundred million/g, coliform group count≤100/g, roundworm egg is dead
Rate >=95%, heavy metal content index meets NY/T798-2004 regulations.
By 20 parts of flyash (100 mesh pulvis), 20 parts of humic acid (100 mesh pulvis), the 60 parts of mixing of middle level complex compound, obtain
Mixture d, natural air drying (water content is 30%), is sieved with 100 mesh vibratory sieves and obtains pulvis siliceous fertilizer product at normal temperatures.
This product is a kind of organosilicon K compound fertilizer with special molecular structure and notable biological activity.Its main spy
Point：
(1) suitable seed dressing, seedbed, nutritive cube, nursery, promote plant root cells mitosis, strengthen photosynthesis, promote
The biosynthesis of protein.Seedbed and crop field base fertilizer can be applied to by appropriate proportioning, using convenient；
(2) it is easy to the compounding of various nutrient elements, is used as nutrition regulating agent and soil conditioner, by root system and leaf
Piece constantly forms carbohydrate, is crop deposit nutrient；
(3) increase Available Silicon In Soils, humic acid and the content of organic matter, effectively alleviate and prevent to draw because of agricultural chemicals, herbicide etc.
The pest and disease damage for rising, promotes various plant physiology metabolism, increases logical plant water vein and improves yield, improving quality.
Using the pulvis siliceous fertilizer (multifunctional bio-organic fertilizer) of the present embodiment as base fertilizer, reality is carried out to soybean crops
Test, as a result referring to table 4.
Table 4
Conspicuousness：T=3.74 ＞ t (001)=2.97
Corn crop is tested using the pulvis siliceous fertilizer (multifunctional bio-organic fertilizer) of the present embodiment, as a result referring to
Table 5.
Table 5
6th, the preparation of particle siliceous fertilizer
The bottom sediment part that above-mentioned fermentation system is produced is silicon element slag (canescence), and pH value about 9.5 (alkalescence) is effectively living
Bacterium number (cfu) >=0.2 hundred million/g, coliform group count≤100/g, induced worm egg death rate >=95%, heavy metal content index meets
NY/884-2012 specifies.
30 parts of humic acid (100 mesh pulvis), careless 30 parts of carbon (100 mesh pulvis), 5 parts of clay (100 mesh pulvis), the earthworm become thoroughly decomposed
15 parts of earthworm excrement (100 mesh pulvis), bottom silicon 20 parts of slag of element, is stirred with mixer, obtains Mischung；
100 mesh are crushed after silicon element slag is air-dried, is stirred with Mischung and is added 3~5% silicone fluids by granulation requirement, entered
Row granulation, drying, sub-sieve obtain particle fertilizer product.Its main feature：
(1) suitable field crops apply fertilizer to the subsoil, seed manure and topdress, and can also be beneficial to the application and large area fertilising of plant equipment
Operation, prevents the loss of N, P, K fertilizer, increases fertilizer efficiency；
(2) alleviate heavy metal to poison crops and the pollution to soil, enhancing plant tissue mechanical strength, disease resistance,
It is resistant to lodging；
(3) more preferably, element silicon forms silicrete to basal dressing liquid feeding fertilizer spray effect from crop root to cauline leaf, strengthens plant
To the protective barrier of pest and disease damage, saving water, resisting drought, raising salt resistance of plants.
Paddy rice is tested using the particle siliceous fertilizer (multifunctional bio-organic fertilizer) of the present embodiment, as a result referring to table 6.
Table 6
Numeral is the average value of three repetitions, graceful work in table：F=1.751.63 ＞ F (0.01)=9.78
Wheat is tested using the particle siliceous fertilizer (multifunctional bio-organic fertilizer) of the present embodiment, as a result referring to table 7.
Table 7
Embodiment 2
1st, the selection of strain
A, silicate bacteria (Bacillus mucilaginosus) Accc10012；
B, black-koji mould (Aspergillus niger), purchased from Harbin Zhenyang river soy sauce, vinegar food factory, it is the factory
Food-grade aspergillus niger for producing amylase, protease, carbohydrase, the enzyme preparation of pectic acid；
C, bacillus licheniformis (Bacillus licheniformis) Accc11080；
D, bacillus subtilis (Bacillus subtilis) Accc11025；
E, Lactococcus lactis (Lactoccus llctis subsp lactjs) Accc11016；
F, Lactobacillus plantarum (Lactobacillut plantarum Holland) P-8；
G, saccharomyces cerevisiae (Saccharomyces cerevisiae), are to commonly use in beer preparation technology purchased from brewery
Saccharomyces cerevisiae；
H, B. mucilaginocus (potassium bacteria), filter out according to prior art from tobacco rhizosphere soil, through mirror
It is fixed not find that there were significant differences with traditional B. mucilaginocus, according to traditional B. mucilaginocus culture.
2nd, individual plant fermentation, the suggestion of culture medium and fermentation condition reference strain supplier are carried out above-mentioned strain.Each strain
Can ferment to viable count and reach 5~1,000,000,000/gram or 5~1,000,000,000/mL concentrations above, as seed liquor.Each seed liquor is pressed afterwards
According to silicate bacteria (Bacillus mucilaginosus), black-koji mould (Aspergillus niger), bacillus licheniformis
(Bacillus licheniformis), bacillus subtilis (Bacillus subtilis), Lactococcus lactis (Lactoccus
Llctis subsplactjs), Lactobacillus plantarum (Lactobacillut plantarum Holland), alcohol yeast
(Saccharomyces cerevisiae), B. mucilaginocus (potassium bacteria) viable count about 30：25：8：8：8：8：
13：8 ratio mixing, the soybean milk using solid content 8% is used as nutrient solution, wherein adding about 2% yeast extract, inoculation is mixed
Bacterium solution (inoculum concentration about 10%) is closed, in being fermented under 30 DEG C of anaerobic conditions, is fermented about 20 days, obtain the acid fermentation of pH value about 3.6
Bacterium solution, is that high-activity biological digests liquid (stand-by) wherein having certain selectivity rich in various biology enzymes, to enzymolysis element silicon.
The 3rd, feldspar in powder is broken to the bulk of no more than 5cm × 5cm × 5cm, the high-activity biological enzymolysis of step 2 is added to
In liquid, potassium feldspar and enzyme liquid ratio are 1：5,30 DEG C of standing for fermentation are sealed, potassium feldspar can be observed within the 15th day has outstanding in a liquid
Floating, 20 days potassium feldspar leachables are presented splashing type bubble, and potassium feldspar starts cracking, and silica gel suspension is progressively immersed in bacteria liquid,
PH value is incrementally increased.
By standing, enzymatic reaction system double decomposition is settled about 7 days, and it is silica gel that fermentation system layering will produce upper suspension
Liquid, intermediate complex is colloidal silica, and bottom sediment thing is silicon element slag.
4th, the preparation of liquid silicon fertilizer
95 parts of the upper suspension of enzymatic hydrolysis and fermentation generation is leached, 5 parts mixs with humic acid, 28~30 DEG C of standing for fermentation 2 days are filtered
Go out liquid, as liquid silicon fertilizer (composite bacteria liquid).After testing, the supernatant liquid part that above-mentioned fermentation system is produced is silicone fluid
(dark brown), pH value is 11~13 (alkalescence), living bacteria count (cfu) >=200,000,000/g, coliform group count≤100/g, roundworm egg
The death rate >=95%, heavy metal As, Ca, Pb, Cr, Hg content's index meets NY/T798-2004 regulations.
The composite bacteria liquid of the present embodiment is inoculated in the soil of sterilizing respectively with single B. mucilaginocus, is right not connect bacterium
According to thermophilic culture 15 days determines each Soil Available potassium content, is as a result recorded in table 8.
Table 8
5th, the preparation of particle siliceous fertilizer
The middle level complex compound that above-mentioned fermentation system is produced is colloidal silica (brown color goo), is about containing effective silicon composition
37.5%, pH value is about 10 (alkalescence), and living bacteria count (cfu) >=0.5 hundred million/g, coliform group count≤100/g, roundworm egg is dead
Die rate >=95%, heavy metal content index meets NY/T798-2004 regulations.
The bottom sediment part that above-mentioned fermentation system is produced is silicon element slag (canescence), and pH value is about 10.5 (alkalescence), has
Effect viable count (cfu) >=0.2 hundred million/g, coliform group count≤100/g, induced worm egg death rate >=95%, heavy metal content index
Meet NY/884-2012 regulations.
By 10 parts of flyash (100 mesh pulvis), 25 parts of humic acid (100 mesh pulvis), 45 parts of middle level complex compound, bottom silicon element
20 parts of slag, mixing, granulation, drying, sub-sieve obtain particle fertilizer product.
Tobacco leaf is tested using the particle siliceous fertilizer (multifunctional bio-organic fertilizer) of the present embodiment, as a result referring to table 9.
Table 9
Result shows, using multi-functional composite bacterial fertilizer of the invention, can improve the yield of tobacco leaf and the quality of improvement tobacco leaf.
Claims (10)
1. a kind of preparation method of organic silicon fertilizer, the method comprising the steps of：
A, by silicate bacteria (Bacillus mucilaginosus), black-koji mould (Aspergillus niger), lichens bud
Spore bacillus (Bacillus licheniformis), bacillus subtilis (Bacillus subtilis), Lactococcus lactis
(Lactoccus llctis subsplactjs), Lactobacillus plantarum (Lactobacillut plantarum Holland), wine
Smart saccharomycete (Saccharomyces cerevisiae) is according to viable count 30~35：25~30：8~10：8~10：8~10：8
~10：13~15 ratio mixing, in being fermented more than 15 days under 28~37 DEG C of anaerobic conditions, is made the fermentation of pH value 4.0~3.5
Bacterium solution；
B, potassium feldspar is mixed with the zymocyte liquid of step a, the two mixed proportion：Zymocyte liquid weight is 2 with potassium feldspar weight
~6：1；
C, by the mixed system of step b in 28~37 DEG C of closed standing for fermentation more than 20 days, organic silicon fertilizer is obtained.
2. method according to claim 1, wherein, in step b potassium feldspar be it is size-reduced in advance to granularity less than 5cm ×
The stone and/or stone flour of 5cm × 5cm.
3. method according to claim 1 and 2, wherein, potassium feldspar is that sterilized or non-sterilized potassium is long in step b
Stone；Benayl aminopurine is selectively added in the mixed system of step b；
Preferably, the sterilizing of potassium feldspar is carried out in accordance with the following methods：
Potassium feldspar clear water is washed into floating dust thing, spraying surface disinfection with 75% ethanol is placed in being closed in closed environment
More than 8 hours, taking out standing made ethanol volatilize, the potassium feldspar for being sterilized.
4. method according to claim 1, wherein, after sealed fermenting was more than 20 days in step c, fermentation system is produced point
Layer, upper strata is suspension, centre is colloidal thing, bottom is sediment, and the method also includes step：
D, by the upper strata suspension of fermentation system, mesocolloid shape thing, bottom sediment thing any one or more separation, respectively
As organic silicon fertilizer or mixing two or three preparation organic silicon fertilizer therein, or more enter one with one or more therein
Step is mixed with organic silicon fertilizer series of products with other raw materials.
5. method according to claim 4, the method includes following at least one step：
D-1, the upper strata suspension separation by fermentation system, prepare liquid silicon fertilizer；
D-2, the mesocolloid shape thing separation by fermentation system, prepare pulvis siliceous fertilizer；
D-3, the bottom sediment thing separation by fermentation system, prepare particle siliceous fertilizer.
6. method according to claim 5, wherein：When preparing liquid silicon fertilizer, carry out in accordance with the following methods：
Directly it is separated by filtration the upper strata suspension stoste as liquid silicon fertilizer that the fermentation system is produced；
Or 3~8 parts of humic acid, 0~8 part of potassium dihydrogen phosphate are mixed with 90~95 parts of the upper strata suspension of the fermentation system
Close, the liquid of 18~35 DEG C of standing for fermentation after 12~72 hours is liquid silicon fertilizer.
7. method according to claim 5, wherein：When preparing pulvis siliceous fertilizer, carry out in accordance with the following methods：
By 10~30 parts of flyash, 10~30 parts of humic acid, 0~6 part of potassium dihydrogen phosphate, trace element 0~3 part with the fermentation
35~80 parts of mixing of mesocolloid shape thing of system, are prepared into pulvis siliceous fertilizer；
Preferably, with flyash 20%, humic acid 20% for carrier mixes with the mesocolloid shape thing 60% of the fermentation system,
It is prepared into pulvis siliceous fertilizer.
8. method according to claim 5, wherein：When preparing particle siliceous fertilizer, carry out in accordance with the following methods：
20~30 parts of humic acid, careless 20~30 parts of carbon, 5~15 parts of wormcast, 3~6 parts of clay, 0~8 part of trace element with it is described
15~40 parts of the bottom sediment thing mixing of fermentation system, directly under granulation or airtight condition 18~35 DEG C ferment 5~10 days after
Granulation, obtains particle siliceous fertilizer；
Preferably, with humic acid 30%, careless carbon 30%, the wormcast 15% for becoming thoroughly decomposed, clay 5% for carrier and the fermentation system
Bottom sediment thing 20% mix, directly granulation obtain particle siliceous fertilizer.
9. a kind of organic silicon fertilizer, it is prepared according to the method described in any one of claim 1~8.
10. a kind of zymocyte liquid for preparing the organic silicon fertilizer described in claim 9, it is to prepare in accordance with the following methods
's：
By silicate bacteria (Bacillus mucilaginosus), black-koji mould (Aspergillus niger), lichens gemma
Bacillus (Bacillus licheniformis), bacillus subtilis (Bacillus subtilis), Lactococcus lactis
(Lactoccus llctis subsplactjs), Lactobacillus plantarum (Lactobacillut plantarum Holland), wine
Smart saccharomycete (Saccharomyces cerevisiae) is according to viable count 30~35：25~30：8~10：8~10：8~10：8
~10：13~15 ratio mixing, in being fermented more than 15 days under 28~37 DEG C of anaerobic conditions, is made the fermentation of pH value 4.0~3.5
Bacterium solution.
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| CN111909709A (en) * | 2020-09-04 | 2020-11-10 | 湖北理工学院 | Soil conditioner special for artemisia field and preparation method and application thereof |
| CN112174746A (en) * | 2020-10-09 | 2021-01-05 | 赢享(宁夏)管理咨询有限公司 | Production process of double-soluble water-soluble citrate-soluble porous structure composite silicon fertilizer |
| CN112939701A (en) * | 2021-04-20 | 2021-06-11 | 鹤壁亿丰科技有限公司 | Preparation method of bio-organic fertilizer |
| CN114349573A (en) * | 2022-01-26 | 2022-04-15 | 黑龙江鑫福垚科技发展有限公司 | Multifunctional high-silicon fertilizer prepared from Baoqing water-containing fly ash and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909709A (en) * | 2020-09-04 | 2020-11-10 | 湖北理工学院 | Soil conditioner special for artemisia field and preparation method and application thereof |
| CN111909709B (en) * | 2020-09-04 | 2021-06-04 | 湖北理工学院 | Soil conditioner special for artemisia field and preparation method and application thereof |
| CN112174746A (en) * | 2020-10-09 | 2021-01-05 | 赢享(宁夏)管理咨询有限公司 | Production process of double-soluble water-soluble citrate-soluble porous structure composite silicon fertilizer |
| CN112939701A (en) * | 2021-04-20 | 2021-06-11 | 鹤壁亿丰科技有限公司 | Preparation method of bio-organic fertilizer |
| CN114349573A (en) * | 2022-01-26 | 2022-04-15 | 黑龙江鑫福垚科技发展有限公司 | Multifunctional high-silicon fertilizer prepared from Baoqing water-containing fly ash and preparation method thereof |
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