CN107879834A - A kind of agriculture microbial bacterial agent and its preparation method and application - Google Patents

A kind of agriculture microbial bacterial agent and its preparation method and application Download PDF

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Publication number
CN107879834A
CN107879834A CN201710990109.8A CN201710990109A CN107879834A CN 107879834 A CN107879834 A CN 107879834A CN 201710990109 A CN201710990109 A CN 201710990109A CN 107879834 A CN107879834 A CN 107879834A
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parts
biological
fruit peel
bacterial agent
soil
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柯江波
杨移斌
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Environmental Sciences (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Treatment Of Sludge (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The present invention relates to a kind of agriculture microbial bacterial agent, formed by the Raw material processing of following parts by weight:80 100 parts of powder of straw, 10 20 parts of biological activated sludge, digest biological 15 25 parts of residue dry powder, 58 parts of organobentonite, 36 parts of bee glue powder, 12 parts of function bacterium.The agriculture microbial bacterial agent of the present invention can increase the soil organism, improved soil structure, make loosing soil, porosity increase, and capacity mitigates, and promotes the development of microbial activity and crop root.Biological activated sludge is rich in saccharomycete and glucan, glucan is as macromolecular, the effect of with absorption carriage, harmful substance of the absorption carriage in by the soil of chemical contamination, promote recover due to soil over-burden and chemical contamination and lose lac balance, increase geobiont vigor, the formation that organobentonite promotes soil granular structure is introduced simultaneously, improve soil internal void space, coordinate solid, liquid, gas proportions in soil, promote the release of available nutrient, be advantageous to root growth, improve the resistance of crop.

Description

A kind of agriculture microbial bacterial agent and its preparation method and application
Technical field
The present invention relates to a kind of agriculture microbial bacterial agent and its preparation method and application, belong to agricultural biological technical field.
Background technology
Soil is the basic of our human survivals.Nowadays the wound that this piece soil that we live is subject to, must than more serious Corresponding measure need be taken, to carry out soil remediation, is such as ploughed, the appearance of chemical fertilizer is made " distinctions won on the battlefield " for volume increase, but near In order to devote exclusive attention to output over year, blindness, excessive chemical fertilizer input, the use of organic fertilizer is have ignored, causes to add input cost, but Do not increase yield, cause serious infringement to soil on the contrary, it is relatively low that appearance includes organic matter, soil acidification, Secondary salinization Soil degradation phenomenon including change, soil hardening etc., the fertility wretched insufficiency of soil.Especially soil in protected field environment, because setting It is big to apply evaporation capacity, again without effective precipitation, transplants the higher garden crop of economic value in facility in addition, fertilizer application amount is more Greatly, therefore soil environment is more severe in facility.So research soil amelioration techniques, reasonably change to soil in protected field It is good, it is our urgent problems to be solved.
Microorganism has improved soil, discharges plant active factors, promotes plant root growth, strengthens crop resistance, Improve crop yield and quality and it is free from environmental pollution the advantages that, be widely used in agriculture field in recent years.It is contemplated that Develop a kind of agriculture microbial bacterial agent and its preparation method and application.
The content of the invention
It is an object of the invention to solve the deficiencies in the prior art, there is provided a kind of agriculture microbial bacterial agent and preparation method thereof And application, the notable improved soil of agriculture microbial bacterial agent energy, active factors is discharged, promotes the self-regeneration and purification of soil.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of agriculture microbial bacterial agent, the agriculture microbial bacterial agent are formed by the Raw material processing of following parts by weight:
Powder of straw 80-100 parts, biological activated sludge 10-20 parts, digest biological residue dry powder 15-25 parts, organobentonite 5-8 Part, bee glue powder 3-6 parts, function bacterium 1-2 parts;
The biological activated sludge is by the waste water two after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan Obtained after the processing of person's mixed biologic through filtering, squeezing, drying and crushing, the mass content of saccharomycete is not low in biological activated sludge In 0.5%, the mass content of glucan is not less than 0.25%;
The biological residue dry powder of enzymolysis through homogeneous, biological enzymolysis and dries acquisition by fruit peel slag;
The function bacterium is bacillus subtilis, gel-shaped series bacillus, bacillus licheniformis, bacillus megaterium, Xie Dian Any one or a few mixture in afnyloliquefaciens, Brevibacillus laterosporus, streptomyces microflavus and Lactobacillus plantarum.
Preferably, the agriculture microbial bacterial agent is formed by the Raw material processing of following parts by weight:
90 parts of powder of straw, 15 parts of biological activated sludge digest biological 20 parts of residue dry powder, 6 parts of organobentonite, 5 parts of bee glue powder, 1.5 parts of function bacterium.
Preferably, the preparation method of the biological activated sludge is as follows:
(1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
(2)The two is with volume ratio 2:1 ratio mixing, stirring 1-2 hours stand more than 24h to full and uniform, in 30-35 DEG C, Residue natural subsidence;
(3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge.
Preferably, the fruit peel is loquat skin, pineapple peel, Grape Skin, mangrove bark, apple skin, litchi rind, Hami melon skin With any one or a few the mixture in pear skin.
Preferably, the preparation method of the biological residue dry powder of enzymolysis is as follows:
(1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 20-30 times of compound alkali lye leaching 30-45min, fruit peel slag mud is obtained after further squeezing the juice;
(2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 1.5-2.0%, antioxidant 0.5-0.8%, surplus are deionization Water;
(3)Enzymolysis:Step(2)Slurries adjust pH to 4.0-4.5, appropriate complex enzyme is added, in 40-50 DEG C of constant temperature while stirring 1.5-2.0h is digested, is then heated to 80-90 DEG C of enzyme deactivation, the complex enzyme presses enzyme activity ratio 2 by pectase and cellulase:3~1: 1 composition;
(4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry.
Preferably, the compound alkali lye is made up of following parts by weight component:20-30 parts sodium acid carbonate, aminophylline 0.5- 1.2 parts, 1-3 parts alum and 30-40 part water.
Preferably, described compound alkali lye forms 0.75 part of 25 parts of sodium acid carbonates, aminophylline by following parts by weight component, 3 parts of alum and 35 parts of water.
Preferably, step(2)Described in homogenizing fluid percentage by weight composition it is as follows:Water-soluble dextran 1.8%, antioxygen Agent 0.75%, surplus are deionized water, and high-pressure homogeneous condition is:30-35 DEG C of temperature, pressure 20-30Mpa, homogenizing time 30-45min。
A kind of preparation method of agriculture microbial bacterial agent, the preparation method are as follows:
The first step, prepare biological activated sludge
(1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
(2)The two is with volume ratio 2:1 ratio mixing, stirring 1-2 hours stand more than 24h to full and uniform, in 30-35 DEG C, Residue natural subsidence;
(3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge, through 60-80 mesh sieve It is standby;
Second step, prepare and digest biological residue dry powder
(1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 20-30 times of compound alkali lye leaching 30-45min, fruit peel slag mud is obtained after further squeezing the juice;
(2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 1.5-2.0%, antioxidant 0.5-0.8%, surplus are deionization Water;
(3)Enzymolysis:Step(2)Slurries adjust pH to 4.0-4.5, appropriate complex enzyme is added, in 40-50 DEG C of constant temperature while stirring 1.5-2.0h is digested, is then heated to 80-90 DEG C of enzyme deactivation, the complex enzyme presses enzyme activity ratio 2 by pectase and cellulase:3~1: 1 composition;
(4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry, through 60-80 mesh sieving for standby;
3rd step, powder of straw, organobentonite and bee glue powder respectively cross 60-80 mesh sieves, weigh formula ratio organobentonite and Bee glue powder premixes 0.5-1h, and then the powder of straw with formula ratio, biological activated sludge, the biological residue dry powder of enzymolysis and function bacterium are mixed Close uniform.
A kind of application of agriculture microbial bacterial agent, reparation and conditioning for soil.
The beneficial effects of the invention are as follows:The agriculture microbial bacterial agent of the present invention can increase the soil organism, improved soil knot Structure, make loosing soil, porosity increase, capacity mitigates, and promotes the development of microbial activity and crop root.Biological activated sludge Rich in saccharomycete and glucan, glucan has the effect of absorption carriage, soil of the absorption carriage by chemical contamination as macromolecular Harmful substance in earth, promote recover due to soil over-burden and chemical contamination and lose lac balance, increase soil Earth biologos, while the formation that organobentonite promotes soil granular structure is introduced, improve soil internal void space, coordinate Solid, liquid, gas proportions in soil, promote the release of available nutrient, be advantageous to root growth, improve the resistance of crop.Honeybee The addition of rubber powder suppresses the breeding of harmful bacteria in soil, avoids soil nutrient of excessively overdrawing, and is won for the conditioning and reparation of soil Time.
Embodiment
Below by specific embodiment, technical scheme is described in further detail.
Embodiment 1:
A kind of agriculture microbial bacterial agent, the agriculture microbial bacterial agent are formed by the Raw material processing of following parts by weight:Powder of straw 80 Part, 10 parts of biological activated sludge, digest biological 15 parts of residue dry powder, 5 parts of organobentonite, 3 parts of bee glue powder, 1 part of function bacterium;
The biological activated sludge is by the waste water two after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan Obtained after the processing of person's mixed biologic through filtering, squeezing, drying and crushing, the mass content of saccharomycete is not less than in activated sludge 0.5%, the mass content of glucan is not less than 0.25%;
The biological residue dry powder of enzymolysis through homogeneous, biological enzymolysis and dries acquisition by fruit peel slag;
The function bacterium is the mixture of bacillus subtilis and gel-shaped series bacillus.
Specific to the present embodiment, the preparation method of the biological activated sludge is as follows:
(1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
(2)The two is with volume ratio 2:1 ratio mixing, stirring 1-2 hours stand more than 24h to full and uniform, in 30-35 DEG C, Residue natural subsidence;
(3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge.
In the present embodiment, the fruit peel is the mixture of loquat skin and pineapple peel, the biological residue dry powder of enzymolysis Preparation method is as follows:
(1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 20 times of compound alkali lye leachings 45min, fruit peel slag mud is obtained after further squeezing the juice;
(2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 1.5%, antioxidant 0.5%, surplus are deionized water;
(3)Enzymolysis:Step(2)Slurries adjust pH to 4.0, add appropriate complex enzyme, in 40 DEG C while stirring constant temperature digest 1.5h, is then heated to 80 DEG C of enzyme deactivations, and the complex enzyme presses enzyme activity ratio 2 by pectase and cellulase:3 compositions;
(4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry.
In above-mentioned preparation method, the compound alkali lye is made up of following parts by weight component:20 parts of sodium acid carbonates, aminophyllines 0.5 part, 1 part of alum and 30 parts of water.
The preparation method of the agriculture microbial bacterial agent of the present invention is as follows:
The first step, prepare biological activated sludge
(1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
(2)The two is with volume ratio 2:1 ratio mixing, stirring 1 hour stand more than 24h in 30 DEG C to full and uniform, residue from So sedimentation;
(3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge, through 60 mesh sieve it is standby With;
Second step, prepare and digest biological residue dry powder
(1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 20 times of compound alkali lye leachings 30min, fruit peel slag mud is obtained after further squeezing the juice;
(2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 1.5%, antioxidant 0.5%, surplus are deionized water;
(3)Enzymolysis:Step(2)Slurries adjust pH to 4.0, add appropriate complex enzyme, in 40 DEG C while stirring constant temperature digest 1.5h, is then heated to 80 DEG C of enzyme deactivations, and the complex enzyme presses enzyme activity ratio 2 by pectase and cellulase:3 compositions;
(4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry, through 60 mesh sieving for standby;
3rd step, powder of straw, organobentonite and bee glue powder cross 60 mesh sieves respectively, are ready for the biology of formula ratio and formula ratio Activated sludge, the biological residue dry powder of enzymolysis and function bacterium are well mixed.
Embodiment 2:
A kind of agriculture microbial bacterial agent, the agriculture microbial bacterial agent are formed by the Raw material processing of following parts by weight:Powder of straw 100 Part, 20 parts of biological activated sludge, digest biological 25 parts of residue dry powder, 8 parts of organobentonite, 6 parts of bee glue powder, 2 parts of function bacterium;
The biological activated sludge is by the waste water two after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan Obtained after the processing of person's mixed biologic through filtering, squeezing, drying and crushing, the mass content of saccharomycete is not less than in activated sludge 0.5%, the mass content of glucan is not less than 0.25%;
The biological residue dry powder of enzymolysis through homogeneous, biological enzymolysis and dries acquisition by fruit peel slag;
The function bacterium is the mixture of bacillus licheniformis and bacillus megaterium.
Specific to the present embodiment, the preparation method of the biological activated sludge is as follows:
(1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
(2)The two is with volume ratio 2:1 ratio mixing, stirring 2 hours stand more than 24h in 35 DEG C to full and uniform, residue from So sedimentation;
(3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge.
In the present embodiment, the fruit peel is the mixture of Grape Skin, mangrove bark and apple skin, the biological residue of enzymolysis The preparation method of dry powder is as follows:
(1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 30 times of compound alkali lye leachings 30min, fruit peel slag mud is obtained after further squeezing the juice;
(2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 2.0%, antioxidant 0.8%, surplus are deionized water;
(3)Enzymolysis:Step(2)Slurries adjust pH to 4.5, add appropriate complex enzyme, in 50 DEG C while stirring constant temperature digest 2.0h, is then heated to 90 DEG C of enzyme deactivations, and the complex enzyme presses enzyme activity ratio 1 by pectase and cellulase:1 composition;
(4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry.
In above-mentioned preparation method, the compound alkali lye is made up of following parts by weight component:30 parts of sodium acid carbonates, ammonia tea 1.2 parts of alkali, 3 parts of alum and 40 parts of water.
The preparation method of the agriculture microbial bacterial agent of the present invention is as follows:
The first step, prepare biological activated sludge
(1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
(2)The two is with volume ratio 2:1 ratio mixing, stirring 2 hours stand more than 24h in 35 DEG C to full and uniform, residue from So sedimentation;
(3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge, through 80 mesh sieve it is standby With;
Second step, prepare and digest biological residue dry powder
(1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 30 times of compound alkali lye leachings 45min, fruit peel slag mud is obtained after further squeezing the juice;
(2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 2.0%, antioxidant 0.8%, surplus are deionized water;
(3)Enzymolysis:Step(2)Slurries adjust pH to 4.5, add appropriate complex enzyme, in 50 DEG C while stirring constant temperature digest 2.0h, is then heated to 90 DEG C of enzyme deactivations, and the complex enzyme presses enzyme activity ratio 1 by pectase and cellulase:1 composition;
(4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry, through 80 mesh sieving for standby;
3rd step, powder of straw, organobentonite and bee glue powder cross 80 mesh sieves respectively, are ready for the biology of formula ratio and formula ratio Activated sludge, the biological residue dry powder of enzymolysis and function bacterium are well mixed.
The agriculture microbial bacterial agent of the present invention can increase the soil organism, improved soil structure, make loosing soil, porosity Increase, capacity mitigate, and promote the development of microbial activity and crop root.Biological activated sludge is rich in saccharomycete and glucan, Glucan has the effect of absorption carriage, harmful substance of the absorption carriage in by the soil of chemical contamination, promoted as macromolecular Recover due to soil over-burden and chemical contamination and lose lac balance, increase geobiont vigor, introduce simultaneously Organobentonite promotes the formation of soil granular structure, improves soil internal void space, coordinates solid, liquid, gas three in soil Ratio, promote the release of available nutrient, be advantageous to root growth, improve the resistance of crop.The addition of bee glue powder suppresses soil The breeding of middle harmful bacteria, soil nutrient of excessively overdrawing is avoided, gained time for the conditioning and reparation of soil.
Embodiment described above is a kind of preferable scheme of the present invention, not the present invention is made any formal Limitation, there are other variants and remodeling on the premise of without departing from the technical scheme described in claim.

Claims (10)

  1. A kind of 1. agriculture microbial bacterial agent, it is characterised in that:The agriculture microbial bacterial agent by following parts by weight Raw material processing Form:
    Powder of straw 80-100 parts, biological activated sludge 10-20 parts, digest biological residue dry powder 15-25 parts, organobentonite 5-8 Part, bee glue powder 3-6 parts, function bacterium 1-2 parts;
    The biological activated sludge is by the waste water two after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan Obtained after the processing of person's mixed biologic through filtering, squeezing, drying and crushing, the mass content of saccharomycete is not low in biological activated sludge In 0.5%, the mass content of glucan is not less than 0.25%;
    The biological residue dry powder of enzymolysis through homogeneous, biological enzymolysis and dries acquisition by fruit peel slag;
    The function bacterium is bacillus subtilis, gel-shaped series bacillus, bacillus licheniformis, bacillus megaterium, Xie Dian Any one or a few mixture in afnyloliquefaciens, Brevibacillus laterosporus, streptomyces microflavus and Lactobacillus plantarum.
  2. A kind of 2. agriculture microbial bacterial agent according to claim 1, it is characterised in that:The agriculture microbial bacterial agent is under The Raw material processing of row parts by weight forms:
    90 parts of powder of straw, 15 parts of biological activated sludge digest biological 20 parts of residue dry powder, 6 parts of organobentonite, 5 parts of bee glue powder, 1.5 parts of function bacterium.
  3. A kind of 3. agriculture microbial bacterial agent according to claim 1 or 2, it is characterised in that:The biological activated sludge Preparation method is as follows:
    (1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
    (2)The two is with volume ratio 2:1 ratio mixing, stirring 1-2 hours stand more than 24h to full and uniform, in 30-35 DEG C, Residue natural subsidence;
    (3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge.
  4. A kind of 4. agriculture microbial bacterial agent according to claim 1, it is characterised in that:The fruit peel is loquat skin, spinach Any one or a few mixture in trailing plants skin, Grape Skin, mangrove bark, apple skin, litchi rind, Hami melon skin and pear skin.
  5. A kind of 5. agriculture microbial bacterial agent according to claim 4, it is characterised in that:The biological residue dry powder of enzymolysis Preparation method is as follows:
    (1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 20-30 times of compound alkali lye leaching 30-45min, fruit peel slag mud is obtained after further squeezing the juice;
    (2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 1.5-2.0%, antioxidant 0.5-0.8%, surplus are deionization Water;
    (3)Enzymolysis:Step(2)Slurries adjust pH to 4.0-4.5, appropriate complex enzyme is added, in 40-50 DEG C of constant temperature while stirring 1.5-2.0h is digested, is then heated to 80-90 DEG C of enzyme deactivation, the complex enzyme presses enzyme activity ratio 2 by pectase and cellulase:3~1: 1 composition;
    (4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry.
  6. A kind of 6. agriculture microbial bacterial agent according to claim 5, it is characterised in that:The compound alkali lye is by following weight Number component forms:20-30 parts sodium acid carbonate, aminophylline 0.5-1.2 parts, 1-3 parts alum and 30-40 part water.
  7. 7. the processing method of the chromium-rich mangrove bark dietary-fiber biscuit of selenium-rich according to claim 6, it is characterised in that:It is described Compound alkali lye 0.75 part of 25 parts of sodium acid carbonates, aminophylline, 3 parts of alum and 35 parts of water are formed by following parts by weight component.
  8. A kind of 8. agriculture microbial bacterial agent according to claim 5, it is characterised in that:Step(2)Described in homogenizing fluid Percentage by weight composition is as follows:Water-soluble dextran 1.8%, antioxidant 0.75%, surplus are deionized water, high-pressure homogeneous bar Part is:30-35 DEG C of temperature, pressure 20-30Mpa, homogenizing time 30-45min.
  9. A kind of a kind of 9. preparation method of agriculture microbial bacterial agent described in claim 1, it is characterised in that:The preparation method It is as follows:
    The first step, prepare biological activated sludge
    (1)Collect the waste water after saccharomycete biofermentation waste water and yeast cell wall alkalinity extraction glucan;
    (2)The two is with volume ratio 2:1 ratio mixing, stirring 1-2 hours stand more than 24h to full and uniform, in 30-35 DEG C, Residue natural subsidence;
    (3)Collection step(2)Residue, it is filtered, squeezing, dry and crush obtain biological activated sludge, through 60-80 mesh sieve It is standby;
    Second step, prepare and digest biological residue dry powder
    (1)Fruit peel pre-processes:Fruit peel slag is collected, removes core and stubble in fruit peel slag, in 20-30 times of compound alkali lye leaching 30-45min, fruit peel slag mud is obtained after further squeezing the juice;
    (2)Fruit peel slag mud homogeneous:The high-pressure homogeneous acquisition slurry of fruit peel slag mud, appropriate homogenizing fluid is added in homogeneous, it is described The percentage by weight composition of homogenizing fluid is as follows:Water-soluble dextran 1.5-2.0%, antioxidant 0.5-0.8%, surplus are deionization Water;
    (3)Enzymolysis:Step(2)Slurries adjust pH to 4.0-4.5, appropriate complex enzyme is added, in 40-50 DEG C of constant temperature while stirring 1.5-2.0h is digested, is then heated to 80-90 DEG C of enzyme deactivation, the complex enzyme presses enzyme activity ratio 2 by pectase and cellulase:3~1: 1 composition;
    (4)Step(3)Enzymolysis liquid must digest biological residue dry powder through dry, through 60-80 mesh sieving for standby;
    3rd step, powder of straw, organobentonite and bee glue powder respectively cross 60-80 mesh sieves, weigh formula ratio organobentonite and Bee glue powder premixes 0.5-1h, and then the powder of straw with formula ratio, biological activated sludge, the biological residue dry powder of enzymolysis and function bacterium are mixed Close uniform.
  10. A kind of a kind of 10. application of agriculture microbial bacterial agent described in claim 1, it is characterised in that:Reparation for soil With conditioning.
CN201710990109.8A 2017-10-23 2017-10-23 A kind of agriculture microbial bacterial agent and its preparation method and application Pending CN107879834A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108503474A (en) * 2018-07-10 2018-09-07 潍坊华潍新材料科技有限公司 A kind of soil conditioner and its preparation method and application
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CN110121971A (en) * 2019-04-24 2019-08-16 广西博世科环保科技股份有限公司 A kind of method of metal mine acidity discarded ground ecological recovery
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CN108719335A (en) * 2018-05-07 2018-11-02 柯江波 Sustained release bacteriostatic agent and preparation method for Vegetable culture medium processing
CN108503474A (en) * 2018-07-10 2018-09-07 潍坊华潍新材料科技有限公司 A kind of soil conditioner and its preparation method and application
CN110627577A (en) * 2019-03-13 2019-12-31 扬州博轩生物科技有限公司 Compound formula of microbial agent for effectively improving soil and preparation method thereof
CN110121971A (en) * 2019-04-24 2019-08-16 广西博世科环保科技股份有限公司 A kind of method of metal mine acidity discarded ground ecological recovery

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