CN108048097B - Beneficial microorganism accelerant for seaweed soil - Google Patents
Beneficial microorganism accelerant for seaweed soil Download PDFInfo
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- CN108048097B CN108048097B CN201711433440.6A CN201711433440A CN108048097B CN 108048097 B CN108048097 B CN 108048097B CN 201711433440 A CN201711433440 A CN 201711433440A CN 108048097 B CN108048097 B CN 108048097B
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
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- C—CHEMISTRY; METALLURGY
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Abstract
The invention discloses a beneficial microorganism accelerant for seaweed soil, which is prepared from the following raw materials in parts by weight: 100-120 parts of alginic acid, 60-80 parts of chitosan oligosaccharide, 10-20 parts of inulin, 5-10 parts of fructo-oligosaccharide, 3-5 parts of polylysine, 2-4 parts of garlic polysaccharide, 5-10 parts of algal polysaccharide and 0.5 part of cysteine. The beneficial microorganism accelerant for the seaweed soil is reasonable in formula, and the components have synergistic effect, so that the beneficial microorganism flora in the soil can be well promoted to be increased, and a reasonable soil flora structure is formed.
Description
Technical Field
The invention belongs to the technical field of soil improvement, and particularly relates to a beneficial microorganism accelerant for seaweed soil.
Background
Soil is one of the main resources depending on human survival and is also an important component of the human ecological environment. The soil contained 45% inorganic, 5% organic, 25% moisture and 25% air. The soil is usually stratified, with the top layer being the top layer, which contains humus, plant roots and living animals (such as microorganisms and earthworms), the more humus, the more fertile the top layer. On certain ground surfaces, such as forests, they have a lot of humus and form a barrier. The subsoil, which is below the topsoil, may contain a greater proportion of clay and less organic matter. Below the subsoil is weathered rock and further down is a hard bed.
The soil structure is the size of soil solid phase particles (including aggregates) and the form of spatial arrangement thereof, which not only affects the storage and supply capacity of soil moisture and nutrients required by plant growth, but also affects gas communication, heat balance, microbial activity, root system extension and the like in the soil.
Soil is an open system, so that many microorganisms live in soil, and are divided into two major groups, namely beneficial microbial flora and disease microbial flora, according to the action and relationship of the microorganisms on plants. The beneficial microbial flora is the major force in soil, can decompose organic matters and inorganic salts in the soil into protein and amino acid required by plants under the action of metabolic product enzyme of the beneficial microbial flora, and simultaneously, the propagation and metabolic products of the beneficial microbial flora are also protein and amino acid, and the existence of the substances forms a small protein and amino acid processing plant at the roots of the plants to protect the healthy growth of the plants. The establishment and maintenance of the beneficial microbial flora can promote the growth of the root system and the exertion of the functions of the beneficial microbial flora, and on the contrary, if the disease microbial flora occupies a dominant position, the root system is underdeveloped, the function of the root system is damaged, the overground part growth is inhibited, and the yield and the quality of crops are reduced.
Disclosure of Invention
The beneficial microorganism accelerant for the seaweed soil provided by the invention can well promote the increase of beneficial microorganism flora in the soil and form a reasonable soil flora structure.
The purpose of the invention is realized by the following technical scheme:
a beneficial microorganism accelerant for seaweed soil is prepared from the following raw materials in parts by weight: 100-120 parts of alginic acid, 60-80 parts of chitosan oligosaccharide, 10-20 parts of inulin, 5-10 parts of fructo-oligosaccharide, 3-5 parts of polylysine, 2-4 parts of garlic polysaccharide, 5-10 parts of algal polysaccharide and 0.5 part of cysteine.
Preferably, the beneficial microorganism accelerant for the seaweed soil is prepared from the following raw materials in parts by weight: 110 parts of alginic acid, 70 parts of chitosan oligosaccharide, 15 parts of inulin, 8 parts of fructo-oligosaccharide, 4 parts of polylysine, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharide and 0.5 part of cysteine.
The molecular weight of the alginic acid is 100-150 KDa.
The garlic polysaccharide is prepared by the following steps:
1) cleaning fresh garlic, removing garlic skin, and crushing to obtain garlic pulp;
2) adding 6 times of water into the garlic pulp, steaming at 90 deg.C for 1 hr, keeping the temperature for 0.5 hr, naturally cooling to room temperature, centrifuging, and collecting supernatant;
3) filtering the supernatant with ultrafiltration membrane with molecular weight of 10-20 KDa to obtain crude extractive solution;
4) adjusting pH of the crude extractive solution to neutral, decolorizing with active carbon, and keeping the temperature at 70 deg.C for 20min to obtain extractive solution;
5) concentrating the extractive solution at 60 deg.C under reduced pressure, and spray drying to obtain Bulbus Allii polysaccharide.
The algal polysaccharide has sulfate radical content not higher than 30% and molecular weight of 50KDa-100 KDa.
The invention has the beneficial effects that: the beneficial microorganism accelerant for the seaweed soil is reasonable in formula, and the components have synergistic effect, so that the beneficial microorganism flora in the soil can be well promoted to be increased, and a reasonable soil flora structure is formed; the advantages of the flora are exerted, the soil fertility is improved, a good crop growth environment is formed, the development of root systems is promoted, the growth and division of cells are promoted, the synthesis of nucleic acid and protein is promoted, the supply of water and nutrients of the roots to the overground part and active substances such as cytokinin is ensured, the normal activity of the overground part of the plant is enhanced, and the crop yield is obviously improved; inhibiting pathogenic microorganism, and reducing pest and disease damage.
Detailed Description
The invention is illustrated below with reference to specific examples. It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention in any way.
The experimental procedures of the following examples are conventional unless otherwise specified. The raw materials and reagent materials used in the following examples are all commercially available products unless otherwise specified.
The polymerization degree of the chitosan oligosaccharide is between 2 and 20, and the molecular weight is less than or equal to 3200 Da.
The molecular weight of the polylysine is more than 3 KDa.
Example 1
A beneficial microorganism accelerant for seaweed soil is prepared from the following raw materials in parts by weight: 110 parts of alginic acid, 70 parts of chitosan oligosaccharide, 15 parts of inulin, 8 parts of fructo-oligosaccharide, 4 parts of polylysine, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharide and 0.5 part of cysteine.
The molecular weight of the alginic acid is 100-150 KDa.
The garlic polysaccharide is prepared by the following steps:
1) cleaning fresh garlic, removing garlic skin, and crushing to obtain garlic pulp;
2) adding 6 times of water into the garlic pulp, steaming at 90 deg.C for 1 hr, keeping the temperature for 0.5 hr, naturally cooling to room temperature, centrifuging, and collecting supernatant;
3) filtering the supernatant with ultrafiltration membrane with molecular weight of 10-20 KDa to obtain crude extractive solution;
4) adjusting pH of the crude extractive solution to neutral, decolorizing with active carbon, and keeping the temperature at 70 deg.C for 20min to obtain extractive solution;
5) concentrating the extractive solution at 60 deg.C under reduced pressure, and spray drying to obtain Bulbus Allii polysaccharide.
The algal polysaccharide has sulfate radical content not higher than 30% and molecular weight of 50KDa-100 KDa.
Example 2
A beneficial microorganism accelerant for seaweed soil is prepared from the following raw materials in parts by weight: 100 parts of alginic acid, 80 parts of chitosan oligosaccharide, 20 parts of inulin, 10 parts of fructo-oligosaccharide, 3 parts of polylysine, 2 parts of garlic polysaccharide, 5 parts of algal polysaccharide and 0.5 part of cysteine.
The molecular weight of the alginic acid is 100-150 KDa.
The garlic polysaccharide is prepared by the following steps:
1) cleaning fresh garlic, removing garlic skin, and crushing to obtain garlic pulp;
2) adding 6 times of water into the garlic pulp, steaming at 90 deg.C for 1 hr, keeping the temperature for 0.5 hr, naturally cooling to room temperature, centrifuging, and collecting supernatant;
3) filtering the supernatant with ultrafiltration membrane with molecular weight of 10-20 KDa to obtain crude extractive solution;
4) adjusting pH of the crude extractive solution to neutral, decolorizing with active carbon, and keeping the temperature at 70 deg.C for 20min to obtain extractive solution;
5) concentrating the extractive solution at 60 deg.C under reduced pressure, and spray drying to obtain Bulbus Allii polysaccharide.
The algal polysaccharide has sulfate radical content not higher than 30% and molecular weight of 50KDa-100 KDa.
Example 3
A beneficial microorganism accelerant for seaweed soil is prepared from the following raw materials in parts by weight: 120 parts of alginic acid, 60 parts of chitosan oligosaccharide, 10 parts of inulin, 5 parts of fructo-oligosaccharide, 5 parts of polylysine, 4 parts of garlic polysaccharide, 10 parts of algal polysaccharide and 0.5 part of cysteine.
The molecular weight of the alginic acid is 100-150 KDa.
The garlic polysaccharide is prepared by the following steps:
1) cleaning fresh garlic, removing garlic skin, and crushing to obtain garlic pulp;
2) adding 6 times of water into the garlic pulp, steaming at 90 deg.C for 1 hr, keeping the temperature for 0.5 hr, naturally cooling to room temperature, centrifuging, and collecting supernatant;
3) filtering the supernatant with ultrafiltration membrane with molecular weight of 10-20 KDa to obtain crude extractive solution;
4) adjusting pH of the crude extractive solution to neutral, decolorizing with active carbon, and keeping the temperature at 70 deg.C for 20min to obtain extractive solution;
5) concentrating the extractive solution at 60 deg.C under reduced pressure, and spray drying to obtain Bulbus Allii polysaccharide.
The algal polysaccharide has sulfate radical content not higher than 30% and molecular weight of 50KDa-100 KDa.
Comparative example 1
A beneficial microorganism accelerant for seaweed soil is prepared from the following raw materials in parts by weight: 110 parts of alginic acid, 15 parts of inulin, 8 parts of fructo-oligosaccharide, 4 parts of polylysine, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharide and 0.5 part of cysteine.
The molecular weight of the alginic acid is 100-150 KDa.
The garlic polysaccharide is prepared by the following steps:
1) cleaning fresh garlic, removing garlic skin, and crushing to obtain garlic pulp;
2) adding 6 times of water into the garlic pulp, steaming at 90 deg.C for 1 hr, keeping the temperature for 0.5 hr, naturally cooling to room temperature, centrifuging, and collecting supernatant;
3) filtering the supernatant with ultrafiltration membrane with molecular weight of 10-20 KDa to obtain crude extractive solution;
4) adjusting pH of the crude extractive solution to neutral, decolorizing with active carbon, and keeping the temperature at 70 deg.C for 20min to obtain extractive solution;
5) concentrating the extractive solution at 60 deg.C under reduced pressure, and spray drying to obtain Bulbus Allii polysaccharide.
The algal polysaccharide has sulfate radical content not higher than 30% and molecular weight of 50KDa-100 KDa.
Comparative example 2
A beneficial microorganism accelerant for seaweed soil is prepared from the following raw materials in parts by weight: 110 parts of alginic acid, 70 parts of chitosan oligosaccharide, 15 parts of inulin, 8 parts of fructo-oligosaccharide, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharide and 0.5 part of cysteine.
The molecular weight of the alginic acid is 100-150 KDa.
The garlic polysaccharide is prepared by the following steps:
1) cleaning fresh garlic, removing garlic skin, and crushing to obtain garlic pulp;
2) adding 6 times of water into the garlic pulp, steaming at 90 deg.C for 1 hr, keeping the temperature for 0.5 hr, naturally cooling to room temperature, centrifuging, and collecting supernatant;
3) filtering the supernatant with ultrafiltration membrane with molecular weight of 10-20 KDa to obtain crude extractive solution;
4) adjusting pH of the crude extractive solution to neutral, decolorizing with active carbon, and keeping the temperature at 70 deg.C for 20min to obtain extractive solution;
5) concentrating the extractive solution at 60 deg.C under reduced pressure, and spray drying to obtain Bulbus Allii polysaccharide.
The algal polysaccharide has sulfate radical content not higher than 30% and molecular weight of 50KDa-100 KDa.
Comparative example 3
A beneficial microorganism accelerant for seaweed soil is prepared from the following raw materials in parts by weight: 110 parts of alginic acid, 70 parts of chitosan oligosaccharide, 15 parts of inulin, 8 parts of fructo-oligosaccharide, 4 parts of polylysine and 0.5 part of cysteine.
The molecular weight of the alginic acid is 100-150 KDa.
Culture medium colony assay
The beneficial microorganism promoters for the soil of the seaweeds prepared in examples 1 to 3 and comparative examples 1 to 3 were added to the culture media respectively, and the specific compositions of the culture media were: 50g of yeast extract, 80g of mannitol, 50g of agar and 20g of marine algae soil beneficial microorganism accelerant. And (3) streaking and inoculating azotobacter and agrobacterium to the 6 culture mediums respectively, culturing for 45 hours at the temperature of 32-37 ℃, observing and counting the average diameter of single colonies of the two bacteria on the culture mediums after the culture is finished, and specifically showing the result in table 1.
TABLE 1 results of the culture Medium colony test
The azotobacter is azotobacter chroococcum with the preservation number of CICC 21686; the agrobacterium is agrobacterium tumefaciens, the accession number is ACCC 10601, and the agrobacterium tumefaciens is a commercially available strain.
Field test of cotton
7 cotton fields with consistent soil characters, uniform and medium fertility and serious cotton wilt and verticillium wilt are selected and divided into test fields 1-6 and control fields, wherein the test fields 1-3 are respectively applied with 25 kg/mu of the marine algae soil beneficial microorganism accelerant prepared in examples 1-3 and 100 kg/mu of the common fertilizer, the test fields 4-6 are respectively applied with 25 kg/mu of the marine algae soil beneficial microorganism accelerant prepared in comparative examples 1-3 and 100 kg/mu of the common fertilizer, and the control fields are applied with 125 kg/mu of the common fertilizer.
Ten sampling methods are adopted in each test field, 100 plants are sampled in each test field, cotton wilt and verticillium wilt are investigated after 50 days of fertilization, morbidity and prevention and treatment rate are recorded and counted, and the results are shown in table 2. The method for calculating the morbidity and the prevention rate is as follows:
incidence (%) = number of diseased plants/number of total investigated plants × 100;
control rate (%) = (control field incidence-test field incidence)/control field incidence x 100.
After harvesting the cotton field, recording the yield of the cotton seeds, and calculating the yield increase rate, wherein the calculation method comprises the following steps:
yield increase (%) = (test field seed cotton yield-control field seed cotton yield)/control field seed cotton yield x 100.
TABLE 2 Cotton field test results
The above description is only of the preferred embodiments of the present invention, and it should be noted that: it will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the principles of the invention and these are intended to be within the scope of the invention.
Claims (1)
1. The beneficial microorganism accelerant for the seaweed soil is characterized by being prepared from the following raw materials in parts by weight: 110 parts of alginic acid, 70 parts of chitosan oligosaccharide, 15 parts of inulin, 8 parts of fructo-oligosaccharide, 4 parts of polylysine, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharide and 0.5 part of cysteine;
the molecular weight of the alginic acid is 100KDa-150 KDa;
the garlic polysaccharide is prepared by the following steps:
1) cleaning fresh garlic, removing garlic skin, and crushing to obtain garlic pulp;
2) adding 6 times of water into the garlic pulp, steaming at 90 deg.C for 1 hr, keeping the temperature for 0.5 hr, naturally cooling to room temperature, centrifuging, and collecting supernatant;
3) filtering the supernatant with ultrafiltration membrane with molecular weight of 10-20 KDa to obtain crude extractive solution;
4) adjusting pH of the crude extractive solution to neutral, decolorizing with active carbon, and keeping the temperature at 70 deg.C for 20min to obtain extractive solution;
5) concentrating the extractive solution at 60 deg.C under reduced pressure, and spray drying to obtain Bulbus Allii polysaccharide;
the algal polysaccharide has sulfate radical content not higher than 30% and molecular weight of 50KDa-100 KDa.
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| CN109734521A (en) * | 2019-03-18 | 2019-05-10 | 赤峰学院 | A kind of fertilizing method for vegetables mineral nutrition balance |
| CN110819359A (en) * | 2019-11-22 | 2020-02-21 | 山东寡糖谷生物科技有限公司 | Method for preparing composite oligosaccharide soil remediation agent |
| CN114395607B (en) * | 2022-03-28 | 2022-07-12 | 卡力(烟台)农业发展有限公司 | Seaweed oligopeptide powder and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| DE102008024968A1 (en) * | 2008-04-15 | 2009-10-22 | Tilco Biochemie Gmbh | Preparation for plants |
| WO2016040564A1 (en) * | 2014-09-12 | 2016-03-17 | Sun Chemical Corporation | Micronutrient fertilizer |
| CN107298601A (en) * | 2017-05-16 | 2017-10-27 | 中国科学院烟台海岸带研究所 | A kind of preparation method of compound marine alga fertilizer |
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| CN101870604B (en) * | 2010-06-02 | 2013-09-11 | 崔玉梅 | Composite oligosaccharide plant growth stimulator and preparation method thereof |
| CN105016930A (en) * | 2015-08-10 | 2015-11-04 | 青岛鲸灵海洋科技有限公司 | Organic fertilizer for promoting growth of profitable strain in soil |
| CN105130705A (en) * | 2015-08-21 | 2015-12-09 | 北京雷力海洋生物新产业股份有限公司 | Soil conditioner and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE102008024968A1 (en) * | 2008-04-15 | 2009-10-22 | Tilco Biochemie Gmbh | Preparation for plants |
| WO2016040564A1 (en) * | 2014-09-12 | 2016-03-17 | Sun Chemical Corporation | Micronutrient fertilizer |
| CN107298601A (en) * | 2017-05-16 | 2017-10-27 | 中国科学院烟台海岸带研究所 | A kind of preparation method of compound marine alga fertilizer |
Non-Patent Citations (2)
| Title |
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| "Agricultural uses of plant biostimulants";Pamela Calvo et al;《Plant and Soil》;20140508;第383卷;第3-41页 * |
| "海藻对不同促生菌生长的影响及应用";李智卫;《中国优秀硕士学位论文全文数据库 农业科技辑》;20110615(第06期);第1-66页 * |
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