CN106941958A - A kind of method for culturing seedlings for hybridizing paper mulberry - Google Patents
A kind of method for culturing seedlings for hybridizing paper mulberry Download PDFInfo
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- CN106941958A CN106941958A CN201710113852.5A CN201710113852A CN106941958A CN 106941958 A CN106941958 A CN 106941958A CN 201710113852 A CN201710113852 A CN 201710113852A CN 106941958 A CN106941958 A CN 106941958A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to agricultural plantation technology field, a kind of method for culturing seedlings for hybridizing paper mulberry, including the sterilization of (1) matrix are disclosed：It will be fitted into after matrix in container for plant growth, the limewash for being again 3% with mass concentration after the alcohol disinfecting for being 75% with mass concentration carries out disinfection to matrix, it is standby after then being covered 5 days with film；Wherein, matrix is mixed by the raw material of following parts by weight：10 15 parts of slow release fertilizer, 8 12 parts of activating phosphorite powder, 5 10 parts of middle sand, 5 10 parts of distillers' grains, 38 parts of fertile soil, 35 parts of diatomite, 38 parts of earthworm powder, 35 parts of rock wool, 35 parts of chitosan, 35 parts of expanded chicken manure；(2) selection of cuttage branch and processing, (3) cuttage, (4) cuttage final-period management.The method of the present invention obtains nursery wood, and survival rate is high, and the breeding paper mulberry selected is adapted to the improved seeds in national mountain areas scope popularizing planting.
Description
Technical field
The invention belongs to agricultural plantation technology field, more particularly to a kind of method for culturing seedlings for hybridizing paper mulberry.
Background technology
Paper mulberry is the distinctive fibre-bearing seeds of China, the extraordinary economic forest of category, and the value such as its bast fiber transfusion is high,
It is drought-resistant barren, it is that deserted mountain stony desertification handles proper seeds, is also help-the-poor campaign, peasant shakes off poverty the important way increased income
Footpath.The blade of paper mulberry is very high containing protein, and 20~30% or so, far above corn, wheat, amino acid, vitamin, carbon aquation
Compound enriches very much, for animal feeding-stuff, bark, and bar is used for papermaking, miscellaneous as country proposes paper mulberry poverty alleviation industry precedence requirement
It is that, through improvement of genes, a kind of fast-growing tree species with excellent resistance of artificial culture, can meet economical, raw to hand over paper mulberry
The many service functions of state, paper mulberry can largely be planted in China marginal land for hybridization, can obtain crude protein Woody feed, solve
Certainly the contradiction on ground is striven in agriculture and animal husbandry, is helped peasant household to shake off poverty and set out on the road to prosperity, can be improved the ecological environment of poverty-stricken area again, in the situation of accurate poverty alleviation
Under, paper mulberry poverty alleviation will be to construct the poverty-stricken area masses and shake off poverty to inject new very strong driving force.Hybridize paper mulberry or rocky mountainous area stone
Desertization administers the seeds high with the economic worth of production of forestry.Hybridization paper mulberry industry is to become the resource inferior position in mountain area to be economical excellent
Gesture, ecological dominance, benefit the economic industry of the rocky mountainous area people, are an item collection economy, society, ecological triple effect beneficial to one
Integrated agriculture industry.However, hybridization paper mulberry nursery is the bottleneck for suppressing hybridization paper mulberry industry, the tissue cultures of hybridization paper mulberry are present
The technical factor such as Multiple Buds difficulty and seedling easily vitrifying, tissue culture is higher to technology, equipment requirement, and fund input amount is big, from
Industry personnel specialty technical requirements height etc., the problems such as common cutting plantation is not high limits its application.
At present, the growing and cultivation of hybridization paper mulberry mainly carries out nursery using cuttage, due to many reasons, rooting rate compared with
Low, growth cycle is long, it is difficult to realize efficiently quickly heavy industrialization growing and cultivation.
The content of the invention
For it is above-mentioned positioned the problem of, goal of the invention of the invention be provide it is a kind of hybridize paper mulberry method for culturing seedlings,
Method by cuttage and seedling culture is obtained nursery wood by the application, and survival rate is high, the breeding structure selected by the application method
Tree is adapted to the improved seeds in national mountain areas scope popularizing planting, protecting ecology balance.
In order to realize foregoing invention purpose, the technical solution adopted by the present invention is as follows：
A kind of method for culturing seedlings for hybridizing paper mulberry, it is characterised in that：Including matrix sterilization, the selection of cuttage branch and processing,
Cuttage and cuttage final-period management；
(1) matrix is sterilized：To be fitted into after matrix in container for plant growth, with mass concentration for 75% alcohol disinfecting after use matter again
Amount concentration carries out disinfection for 3% limewash to matrix, standby after then being covered 5 days with film；
Wherein, matrix is mixed by the raw material of following parts by weight：10-15 parts of slow release fertilizer, activating phosphorite powder 8-
12 parts, middle sand 5-10 parts, 5-10 parts of distillers' grains, 3-8 parts of fertile soil, 3-5 parts of diatomite, 3-8 parts of earthworm powder, 3-5 parts of rock wool, shell gather
Sugared 3-5 parts, expanded 3-5 parts of chicken manure；
(2) selection and processing of cuttage branch, specifically include following processing：
1. the branch for selecting stalwartness is material；
2. the branch chosen is chipped away from hybridization paper mulberry with knife, branch progress amputation has then been left and taken 2-3
Bud point.Then inclined-plane trimming is carried out to the bottom of branch, then is with the clever solution of bacterium or mass concentration by the beveled ends trimmed
25-35% potassium permanganate dilutes 200-500 times and soaked 8-10 minutes, is rinsed after taking-up with clear water, obtains cuttage branch stand-by；
3. sugar is added in ABT takes root the solution that powder content is 1-5mg/L, stirring and dissolving is subsequently added into foregoing mixing and swashed
Plain solution, is subsequently added into acid loess and stirs into slurry of taking root, and will take root slurry natural cooling after high-temperature sterilization 18-25 minutes,
It is stand-by；
Wherein, sugared addition accounts for the 2% of solution weight, and the addition of hormotone solution accounts for the 1% of solution weight；
Wherein, hormotone solution is heteroauxin, 6- benzyls aminoadenine, methyl α-naphthyl acetate according to 10-15:2-3:3-5's
Weight is obtained than mixed configuration；
4. the beveled ends point of the cuttage branch obtained after 2. step is handled dips in slurry of taking root；
(3) cuttage：After being handled by step (1), the centre of the matrix in container for plant growth hold successfully aperture for 3-5 centimetres,
Depth is 3-5 centimetres of matrix cuttage cave, and the central axis in the matrix cuttage cave is horizontal by 35 ° -45 °, by cuttage
The beveled ends cuttage that branch point dips in slurry of taking root enters matrix cuttage cave, then fills up cave, then root water of drenching using river sand；
(5) cuttage final-period management：
A, to after cuttage cuttings carry out seedling in greenhouse, during cuttage rooting the shading rate of greenhouse be 75%, keep base
The moisture content of matter is 60%-70%, and to foliage-spray water, keeps blade face to moisten 4 hours/day, continuous wetness 7-15 days is treated
After blade face rejuvenates, remove the flower the more balance fertilizer for once diluting 800-1000 times is sprayed after sheltering from heat or light completely, 7-10 is spaced afterwards
It is sprayed once, while being encroached at interval of the Bordeaux mixture aqueous solution preventing disease and pest of sprinkling in 7-10 days, during cuttage rooting
It was found that having fallen leaves, the phenomenon dried up of going mouldy or/and change colour is removed in time；
B, after cuttage rooting, the Gibberellins solution for being 1000ppm with concentration sprays cuttings, and interval uses dense after 2-3 days
Spend and spray cuttings for 1000ppm indole -3-butyric acid solution, until the gemma in cuttings grows sprouting, then change spraying concentration
Accelerate the growth of sprouting for 500ppm 6-benzyladenine solution；
Wherein, spray is defined so that cuttings is squirted；
C, the preventing and treating for carrying out rich water quality management and pest and disease damage, can obtain intact hybridization paper mulberry cuttage seeding in 1-2 months.
In the present invention, further instruction, it is described step 3. in, the high-temperature sterilization be temperature be 90-100 DEG C
Under the conditions of carry out disinfection.
In the present invention, further instruction, the slow release fertilizer is made up of following raw material：Lapping 3-5
Part, 10-20 parts of effective fertilizer composition, 4-5 parts of auxiliary material；
Wherein lapping is obtained by mixing by following raw material：5-8 parts of sodium alginate oligosaccharide, 1-3 parts of epoxy resin,
0.5-1.5 parts of xanthans；
The effective fertilizer composition is made up of following raw material：It is trace element, urea, ammonium chloride, tortoise shell, pig testis, white
Granulated sugar, cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek, Gleditsia officinalis deadwood and cellulase；
Include boron fertilizer, cobalt fertilizer, zinc fertilizer, copper fertilizer, molydbenum fertilizer, manganese fertilizer, iron fertilizer in parts by weight；
The auxiliary material includes N- NBPTs and 3- methylpyrazoles.
In the present invention, further instruction, the slow release fertilizer is prepared by following method：
A, by tortoise shell, pig testis crush mix with white granulated sugar keep humidity be 65%, fermentation process 5-8 days obtains egg
White fermentate；
B, cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek and Gleditsia officinalis deadwood be crushed to particle diameter 1-
2cm, is then mixed with cellulase, and fermentation process 2-3 days obtains fiber fermentation thing；
C, protein fermentation thing, fiber fermentation thing and trace element, urea, ammonium chloride is mixed to get effective fertilizer composition；
D, N- NBPTs, 3- methylpyrazoles and effective fertilizer composition be mixed to get first composition fertilizer；
E, first composition fertilizer is sprayed to aqueous solution stirring moistening be defined to not water outlet is held, be then placed in what is constantly rotated
In packing machine, then lapping equably sprayed into by the nozzle on packing machine, until obtaining the granular fertilizer that particle diameter is 2-3mm
Material, is then dried processing, packs and get product.
In the present invention, further instruction, the container for plant growth is wide opening cup；The wide opening cup is bottomless penetrating shape；
The cup of wide opening cup includes at least two layers, and outermost layer is that film, innermost layer are brown paper.
In the present invention, further instruction, the activating phosphorite powder is specifically what is prepared by following methods：Choose
The phosphorus ore slag of Phosphorus Mineral Area is put into broth bouillon, Shaking culture 3 hours at 38 DEG C, then bacterium night progress plate streak is existed
Cultivated on nutrient medium, the condition of culture is that temperature is 37 DEG C, 5-6 hours, then chooses growing way and is presented dimmed
Bacterium colony is configured to bacterium solution, stand-by；By phosphorus content 250-300mg/kg phosphorus ore crushing, ball milling, 100 mesh sieves are crossed, are then added to
In stand-by bacterium solution, Shaking culture is carried out 2 hours under the conditions of temperature is 38 DEG C, you can obtain activating phosphorite powder.
In the present invention, further instruction, the expanded chicken manure is specifically what is prepared by following methods：By chicken manure
It is 50 DEG C of spontaneous fermentation 5-8 hour to build heap and keep internal temperature, and chicken manure then is opened into natural cooling, the then heating in boiler
Thermal cracking 2-3S to 800-900 DEG C, you can obtain expanded chicken manure.
The cutting medium of the present invention is developed according to cuttage seeding, and the active ingredient in composition is greatly improved, wherein, it is organic
Matter 6%~8.2%, nitrogen 11%~13%, phosphoric acid 0.59%~0.85%, potassium oxide 2.8%~4.7%, ammonium nitrogen 354~
395ppm, nitrate nitrogen, 25~37ppm, 98~109ppm of rapid available phosphorus, 320~406ppm of available potassium, wherein, distillers' grains, fertile soil,
Diatomite, filter mud of sugarcane, rock wool, chitosan, expanded chicken manure contain a large amount of organic matters and fibrous matter；Diatomite, expanded chicken manure,
Sugarcane diatomite also has substantial amounts of carbon, nitrogen, P elements；Activating phosphorite powder has more active P elements, holds
Easily it is absorbed and used by plants, ground phosphate rock can not only provide the friendly processing side of P elements or the discarded phosphorus ore slag of a developing
Formula, discarded phosphorus ore slag is relatively low due to its phosphorus content, is not often used to extract P elements, cost is too high, but phosphorus ore slag is random
Heap, which leaves about, directly to be utilized to plant, and only few plant, which can be just grown on phosphorus ore slag, can not make full use of phosphorus ore
P elements on slag.Ground phosphate rock is activated by the application, discarded phosphorus ore slag is regard as culture substratess, activated phosphorusore
Powder, can not only utilize discarded ground phosphate rock, moreover it is possible to the culture substratess made by discarded ground phosphate rock, for activating phosphorite powder,
Kill two birds with one stone.Slow release fertilizer, the slow release fertilizer obtained by the application formula and the processing of method parcel, not only nutrient content is high,
Also reach that slow Releasing nutrient is fully absorbed by cuttage seeding, first, by promoting growth of cuttage seedlings to go out root, not only needed
Nutrient and hormone are excited, in addition it is also necessary to a ventilative matrix environment, middle sand, distillers' grains, expanded chicken manure, the addition of activating phosphorite powder
One ventilative environment can be provided, more conducively hybridize taking root for paper mulberry cuttage seeding, substantial amounts of support is required supplementation with after then taking root
Point, distillers' grains, fertile soil, diatomite, filter mud of sugarcane, rock wool, chitosan, expanded chicken manure can provide nutriment, with culture
The passage of time, root system extension increases, while slow release fertilizer is by Releasing nutrient, slow release fertilizer prepared by the application is different from all
Fertilizer is wrapped up in the core using lapping, but glued fertilizer by lapping even application a thin layer lapping
Knot is good and prepares slow release fertilizer, and the time of its sustained release release fertility is grown not as being wrapped up using lapping, delays
The time of release fertility conforms exactly to the time of the nutrition utilized needed for cuttage seeding, and cuttage seeding also can just absorb enough support
Point, promote root growth, the well developed root system of cuttage seeding can improve the survival rate of cuttage seeding.
Slow release fertilizer in the present invention can be utilized the material in the internal organ of animal there is provided necessary hormone, miscellaneous
Grass and agricultural waste material obtain being rich in a large amount of carbon sources, cellulose, chemical organic compounds and free state by cellulase fermentations
Ion, can by cuttage seeding Root Absorption utilize, trace element is the required nutrient raw material of plant growth, urea, chlorine
Change ammonium as nitrogen source, the auxiliary material (N- NBPTs and 3- methylpyrazoles) of addition suppresses urea activity, can reduced
The generation of ammonia, nitrogenous fertilizer will avoid the occurrence of the nitre state ammonia of high concentration and appearance with nitrite nitrogen, improve utilization rate of nitrogen fertilizer, promote nitrogen
Fertilizer exists in the form of ammonium nitrogen, reduces nitrate nitrogen and nitrite nitrogen form supplies the time of crop；Alleviate crops seedling stage
Poison, the ability of pest and disease damage is resisted in enhancing；The surface of lapping even application fertilizer is prepared into the slow of 2-3 millimeters of particle diameter
Release fertilizer, with can humidity be 65% under conditions of start within 19-21 days release fertility.
By adopting the above-described technical solution, the beneficial effects of the invention are as follows：
(1) cuttage and seedling culture method of the present invention is easily understood, and survival rate is high, nursery stock health and obtained varietal character plants it
After belong to improved seeds, well developed root system is colonized in mountain area plantation, survival rate to more than 90%.
(2) cuttage and seedling culture of the invention uses container for plant growth to carry out nursery, can directly connect after nursery success
Band container for plant growth is buried field planting in the lump, and material is buried in soil and is decomposed afterwards, and bottomless design does not hinder seedling growth, treats the stem of nursery stock
Container for plant growth is decomposed after dry cross growth, and plant, by continued growth, is also to have container for plant growth to exist in firm field planting, cuttage seeding
Root system is forced down to grow so that the viability increase after cuttage seeding field planting, the yield of ginseng will be greatly improved.
(3) processing of the cuttage seeding of the application is will to carry out inclined-plane processing to cuttage seeding, first disinfected using solution, so
Root-inducing powder is first mixed there is provided more capability facfors with sugar afterwards, hormotone solution can excite the factor in cuttage seeding constitution, enzyme
Activity, callus activity are so as to promote the long root of cuttage seeding, and additional that slurry of taking root is stirred into addition of acid loess is good there is provided one
Good pH environment and nutrient environment, promotes cuttage seeding quickly to grow.
Embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, tie below
Embodiment is closed, the present invention is expanded on further.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention,
It is not intended to limit the present invention.
Embodiment 1
First, prepared by raw material：
1. slow release fertilizer is made up of following raw material：1 part of trace element, 1 part of urea, 1 part of ammonium chloride, tortoise shell
0.5 part, 0.5 part of pig testis, 1.5 parts of white granulated sugar, 0.5 part of cocoanut tree skin, 0.5 part of banyan deadwood, 0.5 part of lotus bar, vicia amoena
1.5 parts of 0.5 part of rattan, 0.5 part of wild leek, 0.5 part of Gleditsia officinalis deadwood and cellulase
2.5 parts of N- NBPTs, 2.5 parts of 3- methylpyrazoles, 2 parts of sodium carboxymethylcellulose, 3 parts of cyanogen；
Wherein, trace element includes 1 part of boron fertilizer, 0.5 part of cobalt fertilizer, 1 part of zinc fertilizer, 1 part of copper fertilizer, 0.5 part of molydbenum fertilizer, manganese fertilizer 0.5
Part, 0.5 part of iron fertilizer；
The raw material of said ratio is applied to the following method for preparing slow release fertilizer, specifically included：
A, by tortoise shell, pig testis crush mix with white granulated sugar keep humidity be 65%, fermentation process 5 days obtains albumen
Fermentate；
B, cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek and Gleditsia officinalis deadwood be crushed to particle diameter
1cm, is then mixed with cellulase, and fermentation process 2 days obtains fiber fermentation thing；
C, protein fermentation thing, fiber fermentation thing and trace element, urea, ammonium chloride is mixed to get effective fertilizer composition；
D, N- NBPTs, 3- methylpyrazoles and effective fertilizer composition be mixed to get first composition fertilizer；
E, first composition fertilizer is sprayed to aqueous solution stirring moistening be defined to not water outlet is held, be then placed in what is constantly rotated
In packing machine, then lapping equably sprayed into by the nozzle on packing machine, until obtaining the granular fertilizer that particle diameter is 2mm
Material, is then dried processing, packs and get product.
2. matrix is mixed by the raw material of following parts by weight：10 parts of slow release fertilizer, 8 parts of activating phosphorite powder, middle sand 5
Part, 5 parts of distillers' grains, 3 parts of fertile soil, 3 parts of diatomite, 3 parts of earthworm powder, 3 parts of rock wool, 3 parts of chitosan, 3 parts of expanded chicken manure；
Wherein, activating phosphorite powder is specifically what is prepared by following methods：The phosphorus ore slag for choosing Phosphorus Mineral Area is put into meat soup
In culture medium, Shaking culture 3 hours at 38 DEG C, then bacterium night progress plate streak is cultivated on nutrient medium,
The condition of culture is that temperature is 37 DEG C, 5 hours, then chooses the dimmed bacterium colony of growing way presentation and is configured to bacterium solution, stand-by；It will contain
Phosphorus amount 250mg/kg phosphorus ore is crushed, ball milling, is crossed 100 mesh sieves, is then added in stand-by bacterium solution, is 38 DEG C of conditions in temperature
Lower progress Shaking culture 2 hours, you can obtain activating phosphorite powder.
Wherein, expanded chicken manure is specifically what is prepared by following methods：It is 50 DEG C that chicken manure, which is built heap to keep internal temperature,
Spontaneous fermentation 5 hours, then opens natural cooling by chicken manure, and thermal cracking 2S at 800 DEG C is then heated in boiler, you can
To expanded chicken manure.
2nd, matrix obtained above is applied on following method for culturing seedlings.
After a kind of method for culturing seedlings for hybridizing paper mulberry, including matrix sterilization, the selection of cuttage branch and processing, cuttage and cuttage
Period management；
(1) matrix is sterilized：To be fitted into after matrix in container for plant growth, with mass concentration for 75% alcohol disinfecting after use matter again
Amount concentration carries out disinfection for 10% limewash to matrix, standby after then being covered 5 days with film；
(2) selection and processing of cuttage branch, specifically include following processing：
1. selection maternal plant is good, and the branch without pest and disease damage, semi-lignified；
2. the branch chosen is chipped away from hybridization paper mulberry foundation portion with knife, then the bottom progress inclined-plane of branch repaiied
Cut, then the beveled ends trimmed are soaked 8 minutes with the clever solution of bacterium, is rinsed after taking-up with clear water, is obtained cuttage branch and treat
With；
3. sugar is added in ABT takes root the solution that powder content is 1mg/L, stirring and dissolving is subsequently added into foregoing hormotone
Solution, is subsequently added into acid loess and stirs into slurry of taking root, and the slurry that will take root passes through 18 points of the high-temperature sterilization under the conditions of temperature is 90 DEG C
Natural cooling after clock, it is stand-by；
Wherein, sugared addition accounts for the 2% of solution weight, and the addition of hormotone solution accounts for the 1% of solution weight；
Wherein, hormotone solution is heteroauxin, 6- benzyls aminoadenine, methyl α-naphthyl acetate according to 10:2:3 weight is than mixed
Close what configuration was obtained；
4. the beveled ends point of the cuttage branch obtained after 2. step is handled dips in slurry of taking root；
(3) cuttage：The time of cuttage is annual April, after being handled by step (1), the matrix in container for plant growth
Hold the matrix cuttage cave that aperture is 3 centimetres, depth is 3 centimetres, the central axis and level in the matrix cuttage cave successfully in centre
Face is in 35 °, and the beveled ends cuttage that cuttage branch point is dipped in into slurry of taking root enters matrix cuttage cave, then fills up hole using river sand
Cave, then root water of drenching；
Wherein, container for plant growth is wide opening cup, and bottomless penetrating shape；The cup of wide opening cup includes at least two layers, and outermost layer is
Film, innermost layer are brown paper.
(5) cuttage final-period management：
A, to after cuttage cuttings carry out seedling in greenhouse, during cuttage rooting the shading rate of greenhouse be 75%, keep base
The moisture content of matter is 60%, and to foliage-spray water, keeps blade face to moisten 4 hours/day, continuous wetness 7 days treats that blade face is recovered
After vigor, remove the flower the more balance fertilizer for once diluting 800 times is sprayed after sheltering from heat or light completely, interval is sprayed once for 7 days afterwards, while often
Bordeaux mixture aqueous solution preventing disease and pest infringement of interval sprinkling in 7 days, finds there are fallen leaves, go mouldy and change colour during cuttage rooting
Withered phenomenon is removed in time；
B, after cuttage rooting, the Gibberellins solution for being 1000ppm with concentration sprays cuttings, interval concentration after 2 days
Cuttings is sprayed for 1000ppm indole -3-butyric acid solution, until the gemma in cuttings grows sprouting, then changing spraying concentration is
500ppm 6-benzyladenine solution accelerates the growth of sprouting；
Wherein, spray is defined so that cuttings is squirted；
C, the preventing and treating for carrying out rich water quality management and pest and disease damage, can obtain intact hybridization paper mulberry cuttage seeding in 1 month.
Embodiment 2：
First, prepared by raw material：
1. slow release fertilizer is made up of following raw material：5 parts of trace element, 4 parts of urea, 3 parts of ammonium chloride, tortoise shell 2
Part, 2 parts of pig testis, 0.5 part of white granulated sugar, 1 part of cocoanut tree skin, 1 part of banyan deadwood, 2 parts of lotus bar, 1 part of vicia amoena rattan, wild leek
1.5 parts of 1 part, 1 part of Gleditsia officinalis deadwood and cellulase
5 parts of N- NBPTs, 5 parts of 3- methylpyrazoles, 2 parts of sodium carboxymethylcellulose, cyanoacrylate-second
2 parts of acylated 2 parts of hydroxypropyl cellulose, 2 parts of hydroxyethyl cellulose and methylcellulose；
Wherein, trace element includes 2 parts of boron fertilizer, 3 parts of cobalt fertilizer, 3 parts of zinc fertilizer, 3 parts of copper fertilizer, 3 parts of molydbenum fertilizer, 3 parts of manganese fertilizer, iron fertilizer
3 parts；
The raw material of said ratio is applied to the following method for preparing slow release fertilizer, specifically included：
A, by tortoise shell, pig testis crush mix with white granulated sugar keep humidity be 65%, fermentation process 6 days obtains albumen
Fermentate；
B, cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek and Gleditsia officinalis deadwood be crushed to particle diameter
1.5cm, is then mixed with cellulase, and fermentation process 3 days obtains fiber fermentation thing；
C, protein fermentation thing, fiber fermentation thing and trace element, urea, ammonium chloride is mixed to get effective fertilizer composition；
D, N- NBPTs, 3- methylpyrazoles and effective fertilizer composition be mixed to get first composition fertilizer；
E, first composition fertilizer is sprayed to aqueous solution stirring moistening be defined to not water outlet is held, be then placed in what is constantly rotated
In packing machine, then lapping equably sprayed into by the nozzle on packing machine, until obtaining the granular fertilizer that particle diameter is 3mm
Material, is then dried processing, packs and get product.
2. matrix is mixed by the raw material of following parts by weight：15 parts of slow release fertilizer, 12 parts of activating phosphorite powder, middle sand
10 parts, 10 parts of distillers' grains, 8 parts of fertile soil, 5 parts of diatomite, 8 parts of earthworm powder, 5 parts of rock wool, 5 parts of chitosan, 5 parts of expanded chicken manure；
Wherein, activating phosphorite powder is specifically what is prepared by following methods：The phosphorus ore slag for choosing Phosphorus Mineral Area is put into meat soup
In culture medium, Shaking culture 3 hours at 38 DEG C, then bacterium night progress plate streak is cultivated on nutrient medium,
The condition of culture is that temperature is 37 DEG C, 6 hours, then chooses the dimmed bacterium colony of growing way presentation and is configured to bacterium solution, stand-by；It will contain
Phosphorus amount 300mg/kg phosphorus ore is crushed, ball milling, is crossed 100 mesh sieves, is then added in stand-by bacterium solution, is 38 DEG C of conditions in temperature
Lower progress Shaking culture 2 hours, you can obtain activating phosphorite powder.
Wherein, expanded chicken manure is specifically what is prepared by following methods：It is 50 DEG C that chicken manure, which is built heap to keep internal temperature,
Spontaneous fermentation 8 hours, then opens natural cooling by chicken manure, and thermal cracking 3S at 900 DEG C is then heated in boiler, you can
To expanded chicken manure.
2nd, matrix obtained above is applied on following method for culturing seedlings.
After a kind of method for culturing seedlings for hybridizing paper mulberry, including matrix sterilization, the selection of cuttage branch and processing, cuttage and cuttage
Period management；
(1) matrix is sterilized：To be fitted into after matrix in container for plant growth, with mass concentration for 75% alcohol disinfecting after use matter again
Amount concentration carries out disinfection for 15% limewash to matrix, standby after then being covered 5 days with film；
(2) selection and processing of cuttage branch, specifically include following processing：
1. selection maternal plant is good, and the branch without pest and disease damage, semi-lignified；
2. the branch chosen is chipped away from hybridization paper mulberry foundation portion with knife, then the bottom progress inclined-plane of branch repaiied
Cut, then the beveled ends trimmed are soaked 10 minutes for 1000 times with the clever solution of bacterium, is rinsed after taking-up with clear water, obtains cuttage
Branch is stand-by；
3. sugar is added in ABT takes root the solution that powder content is 5mg/L, stirring and dissolving is subsequently added into foregoing hormotone
Solution, is subsequently added into acid loess and stirs into slurry of taking root, and the slurry that will take root passes through 25 points of the high-temperature sterilization under the conditions of temperature is 100 DEG C
Natural cooling after clock, it is stand-by；
Wherein, sugared addition accounts for the 2% of solution weight, and the addition of hormotone solution accounts for the 1% of solution weight；
Wherein, hormotone solution is heteroauxin, 6- benzyls aminoadenine, methyl α-naphthyl acetate according to 15:3:5 weight is than mixed
Close what configuration was obtained；
4. the beveled ends point of the cuttage branch obtained after 2. step is handled dips in slurry of taking root；
(3) cuttage：The time of cuttage is annual October, after being handled by step (1), the matrix in container for plant growth
Hold the matrix cuttage cave that aperture is 5 centimetres, depth is 5 centimetres, the central axis and level in the matrix cuttage cave successfully in centre
Face is in 45 °, and the beveled ends cuttage that cuttage branch point is dipped in into slurry of taking root enters matrix cuttage cave, then fills up hole using river sand
Cave, then root water of drenching；
Wherein, container for plant growth is wide opening cup, and bottomless penetrating shape；The cup of wide opening cup includes at least two layers, and outermost layer is
Film, innermost layer are brown paper.
(5) cuttage final-period management：
A, to after cuttage cuttings carry out seedling in greenhouse, during cuttage rooting the shading rate of greenhouse be 75%, keep base
The moisture content of matter is 70%, and to foliage-spray water, keeps blade face to moisten 4 hours/day, continuous wetness 15 days treats that blade face is extensive
Bring back to life after power, remove the flower the more balance fertilizer for once diluting 1000 times is sprayed after sheltering from heat or light completely, interval is sprayed once for 10 days afterwards, together
When encroached at interval of the Bordeaux mixture aqueous solution preventing disease and pest of sprinkling in 10 days, find there are fallen leaves, go mouldy during cuttage rooting
Or the withered phenomenon of discoloration is removed in time；
B, after cuttage rooting, the Gibberellins solution for being 1000ppm with concentration sprays cuttings, interval concentration after 3 days
Cuttings is sprayed for 1000ppm indole -3-butyric acid solution, until the gemma in cuttings grows sprouting, then changing spraying concentration is
500ppm 6-benzyladenine solution accelerates the growth of sprouting；
Wherein, spray is defined so that cuttings is squirted；
C, the preventing and treating for carrying out rich water quality management and pest and disease damage, can obtain intact hybridization paper mulberry cuttage seeding in 2 months.
Embodiment 3：
First, prepared by raw material：
1. slow release fertilizer is made up of following raw material：3 parts of trace element, 2 parts of urea, 2 parts of ammonium chloride, tortoise shell
1.5 parts, 1.5 parts of pig testis, 1 part of white granulated sugar, 1 part of cocoanut tree skin, 1.5 parts of banyan deadwood, 1 part of lotus bar, vicia amoena rattan 1.5
Part, 1 part of wild leek, 1 part of Gleditsia officinalis deadwood and 1.5 parts of cellulase
4 parts of N- NBPTs, 5 parts of 3- methylpyrazoles, cyanoacrylate-acetylation hydroxypropyl cellulose 2
2 parts of part, 2 parts of hydroxyethyl cellulose and methylcellulose；
Wherein, trace element includes 0.5 part of boron fertilizer, 1 part of cobalt fertilizer, 0.5 part of zinc fertilizer, 0.5 part of copper fertilizer, 0.5 part of molydbenum fertilizer, manganese fertilizer
1 part, 1 part of iron fertilizer；
The raw material of said ratio is applied to the following method for preparing slow release fertilizer, specifically included：
A, by tortoise shell, pig testis crush mix with white granulated sugar keep humidity be 65%, fermentation process 6 days obtains albumen
Fermentate；
B, cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek and Gleditsia officinalis deadwood be crushed to particle diameter
1cm, is then mixed with cellulase, and fermentation process 3 days obtains fiber fermentation thing；
C, protein fermentation thing, fiber fermentation thing and trace element, urea, ammonium chloride is mixed to get effective fertilizer composition；
D, N- NBPTs, 3- methylpyrazoles and effective fertilizer composition be mixed to get first composition fertilizer；
E, first composition fertilizer is sprayed to aqueous solution stirring moistening be defined to not water outlet is held, be then placed in what is constantly rotated
In packing machine, then lapping equably sprayed into by the nozzle on packing machine, until obtaining the granular fertilizer that particle diameter is 3mm
Material, is then dried processing, packs and get product.
2. matrix is mixed by the raw material of following parts by weight：12 parts of slow release fertilizer, 9 parts of activating phosphorite powder, middle sand 6
Part, 7 parts of distillers' grains, 5 parts of fertile soil, 4 parts of diatomite, 5 parts of earthworm powder, 4 parts of rock wool, 4 parts of chitosan, 4 parts of expanded chicken manure；
Wherein, activating phosphorite powder is specifically what is prepared by following methods：The phosphorus ore slag for choosing Phosphorus Mineral Area is put into meat soup
In culture medium, Shaking culture 3 hours at 38 DEG C, then bacterium night progress plate streak is cultivated on nutrient medium,
The condition of culture is that temperature is 37 DEG C, 5.5 hours, then chooses the dimmed bacterium colony of growing way presentation and is configured to bacterium solution, stand-by；Will
Phosphorus content 265mg/kg phosphorus ore is crushed, ball milling, is crossed 100 mesh sieves, is then added in stand-by bacterium solution, is 38 DEG C of bars in temperature
Shaking culture is carried out under part 2 hours, you can obtain activating phosphorite powder.
Wherein, expanded chicken manure is specifically what is prepared by following methods：It is 50 DEG C that chicken manure, which is built heap to keep internal temperature,
Spontaneous fermentation 7 hours, then opens natural cooling by chicken manure, and thermal cracking 2S at 850 DEG C is then heated in boiler, you can
To expanded chicken manure.
2nd, matrix obtained above is applied on following method for culturing seedlings.
After a kind of method for culturing seedlings for hybridizing paper mulberry, including matrix sterilization, the selection of cuttage branch and processing, cuttage and cuttage
Period management；
(1) matrix is sterilized：To be fitted into after matrix in container for plant growth, with mass concentration for 75% alcohol disinfecting after use matter again
Amount concentration carries out disinfection for 12% limewash to matrix, standby after then being covered 5 days with film；
(2) selection and processing of cuttage branch, specifically include following processing：
1. selection maternal plant is good, and the branch without pest and disease damage, semi-lignified；
2. the branch chosen is chipped away from hybridization paper mulberry foundation portion with knife, then the bottom progress inclined-plane of branch repaiied
Cut, the beveled ends trimmed then are diluted into 800 times with thiophanate methyl solution soaks 6 minutes, is rinsed after taking-up with clear water,
Obtain cuttage branch stand-by；
3. sugar is added in ABT takes root the solution that powder content is 3mg/L, stirring and dissolving is subsequently added into foregoing hormotone
Solution, is subsequently added into acid loess and stirs into slurry of taking root, and the slurry that will take root passes through 20 points of the high-temperature sterilization under the conditions of temperature is 95 DEG C
Natural cooling after clock, it is stand-by；
Wherein, sugared addition accounts for the 2% of solution weight, and the addition of hormotone solution accounts for the 1% of solution weight；
Wherein, hormotone solution is heteroauxin, 6- benzyls aminoadenine, methyl α-naphthyl acetate according to 12:2:4 weight is than mixed
Close what configuration was obtained；
4. the beveled ends point of the cuttage branch obtained after 2. step is handled dips in slurry of taking root；
(3) cuttage：The time of cuttage is annual June, after being handled by step (1), the matrix in container for plant growth
Hold the matrix cuttage cave that aperture is 4 centimetres, depth is 4 centimetres, the central axis and level in the matrix cuttage cave successfully in centre
Face is in 40 °, and the beveled ends cuttage that cuttage branch point is dipped in into slurry of taking root enters matrix cuttage cave, then fills up hole using river sand
Cave, then root water of drenching；
Wherein, container for plant growth is wide opening cup, and bottomless penetrating shape；The cup of wide opening cup includes at least two layers, and outermost layer is
Film, innermost layer are brown paper.
(5) cuttage final-period management：
A, to after cuttage cuttings carry out seedling in greenhouse, during cuttage rooting the shading rate of greenhouse be 75%, keep base
The moisture content of matter is 65%, and to foliage-spray water, keeps blade face to moisten 4 hours/day, continuous wetness 10 days treats that blade face is extensive
Bring back to life after power, remove the flower the more balance fertilizer for once diluting 900 times is sprayed after sheltering from heat or light completely, interval is sprayed once for 8 days afterwards, simultaneously
At interval of the Bordeaux mixture aqueous solution preventing disease and pest infringement of sprinkling in 8 days, find have fallen leaves and discoloration dry during cuttage rooting
Withered phenomenon is removed in time；
B, after cuttage rooting, the Gibberellins solution for being 1000ppm with concentration sprays cuttings, interval concentration after 3 days
Cuttings is sprayed for 1000ppm indole -3-butyric acid solution, until the gemma in cuttings grows sprouting, then changing spraying concentration is
500ppm 6-benzyladenine solution accelerates the growth of sprouting；
Wherein, spray is defined so that cuttings is squirted；
C, the preventing and treating for carrying out rich water quality management and pest and disease damage, can obtain intact hybridization paper mulberry cuttage seeding in 2 months.
Embodiment 4：
First, prepared by raw material：
1. slow release fertilizer is made up of following raw material：3 parts of trace element, 4 parts of urea, 4 parts of ammonium chloride, tortoise shell 1
Part, 0.5 part of pig testis, 1.5 parts of white granulated sugar, 1 part of cocoanut tree skin, 0.5 part of banyan deadwood, 0.5 part of lotus bar, 1 part of vicia amoena rattan,
1.5 parts of 0.5 part of wild leek, 1 part of Gleditsia officinalis deadwood and cellulase
3 parts of N- NBPTs, 4 parts of 3- methylpyrazoles, 3 parts of sodium carboxymethylcellulose, 2 parts of hydroxyethyl cellulose
With 2 parts of methylcellulose；
Wherein, trace element includes 2 parts of boron fertilizer, 2 parts of cobalt fertilizer, 2 parts of zinc fertilizer, 2 parts of copper fertilizer, 2 parts of molydbenum fertilizer, 2 parts of manganese fertilizer, iron fertilizer
2 parts；
The raw material of said ratio is applied to the following method for preparing slow release fertilizer, specifically included：
A, by tortoise shell, pig testis crush mix with white granulated sugar keep humidity be 65%, fermentation process 7 days obtains albumen
Fermentate；
B, cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek and Gleditsia officinalis deadwood be crushed to particle diameter
1.5cm, is then mixed with cellulase, and fermentation process 2 days obtains fiber fermentation thing；
C, protein fermentation thing, fiber fermentation thing and trace element, urea, ammonium chloride is mixed to get effective fertilizer composition；
D, N- NBPTs, 3- methylpyrazoles and effective fertilizer composition be mixed to get first composition fertilizer；
E, first composition fertilizer is sprayed to aqueous solution stirring moistening be defined to not water outlet is held, be then placed in what is constantly rotated
In packing machine, then lapping equably sprayed into by the nozzle on packing machine, until obtaining the granular fertilizer that particle diameter is 3mm
Material, is then dried processing, packs and get product.
2. matrix is mixed by the raw material of following parts by weight：14 parts of slow release fertilizer, 11 parts of activating phosphorite powder, middle sand
8 parts, 8 parts of distillers' grains, 7 parts of fertile soil, 4 parts of diatomite, 7 parts of earthworm powder, 4 parts of rock wool, 4 parts of chitosan, 4 parts of expanded chicken manure；
Wherein, activating phosphorite powder is specifically what is prepared by following methods：The phosphorus ore slag for choosing Phosphorus Mineral Area is put into meat soup
In culture medium, Shaking culture 3 hours at 38 DEG C, then bacterium night progress plate streak is cultivated on nutrient medium,
The condition of culture is that temperature is 37 DEG C, 6 hours, then chooses the dimmed bacterium colony of growing way presentation and is configured to bacterium solution, stand-by；It will contain
Phosphorus amount 280mg/kg phosphorus ore is crushed, ball milling, is crossed 100 mesh sieves, is then added in stand-by bacterium solution, is 38 DEG C of conditions in temperature
Lower progress Shaking culture 2 hours, you can obtain activating phosphorite powder.
Wherein, expanded chicken manure is specifically what is prepared by following methods：It is 50 DEG C that chicken manure, which is built heap to keep internal temperature,
Spontaneous fermentation 6 hours, then opens natural cooling by chicken manure, and thermal cracking 3S at 880 DEG C is then heated in boiler, you can
To expanded chicken manure.
2nd, matrix obtained above is applied on following method for culturing seedlings.
After a kind of method for culturing seedlings for hybridizing paper mulberry, including matrix sterilization, the selection of cuttage branch and processing, cuttage and cuttage
Period management；
(1) matrix is sterilized：To be fitted into after matrix in container for plant growth, with mass concentration for 75% alcohol disinfecting after use matter again
Amount concentration carries out disinfection for 14% limewash to matrix, standby after then being covered 5 days with film；
(2) selection and processing of cuttage branch, specifically include following processing：
1. selection maternal plant is good, and the branch without pest and disease damage, semi-lignified；
2. the branch chosen is chipped away from hybridization paper mulberry foundation portion with knife, then the bottom progress inclined-plane of branch repaiied
Cut, the beveled ends trimmed then are diluted into 1000 times with thiophanate methyl solution soaks 9 minutes, is rushed after taking-up with clear water
Wash, obtain cuttage branch stand-by；
3. sugar is added in ABT takes root the solution that powder content is 4mg/L, stirring and dissolving is subsequently added into foregoing hormotone
Solution, is subsequently added into acid loess and stirs into slurry of taking root, and the slurry that will take root passes through 22 points of the high-temperature sterilization under the conditions of temperature is 97 DEG C
Natural cooling after clock, it is stand-by；
Wherein, sugared addition accounts for the 2% of solution weight, and the addition of hormotone solution accounts for the 1% of solution weight；
Wherein, hormotone solution is heteroauxin, 6- benzyls aminoadenine, methyl α-naphthyl acetate according to 13:3:4 weight is than mixed
Close what configuration was obtained；
4. the beveled ends point of the cuttage branch obtained after 2. step is handled dips in slurry of taking root；
(3) cuttage：The time of cuttage is annual August, after being handled by step (1), the matrix in container for plant growth
Hold the matrix cuttage cave that aperture is 4 centimetres, depth is 4 centimetres, the central axis and level in the matrix cuttage cave successfully in centre
Face is in 42 °, and the beveled ends cuttage that cuttage branch point is dipped in into slurry of taking root enters matrix cuttage cave, then fills up hole using river sand
Cave, then root water of drenching；
Wherein, container for plant growth is wide opening cup, and bottomless penetrating shape；The cup of wide opening cup includes at least two layers, and outermost layer is
Film, innermost layer are brown paper.
(5) cuttage final-period management：
A, to after cuttage cuttings carry out seedling in greenhouse, during cuttage rooting the shading rate of greenhouse be 75%, keep base
The moisture content of matter is 68%, and to foliage-spray water, keeps blade face to moisten 4 hours/day, continuous wetness 12 days treats that blade face is extensive
Bring back to life after power, remove the flower the more balance fertilizer for once diluting 900 times is sprayed after sheltering from heat or light completely, interval is sprayed once for 8 days afterwards, simultaneously
At interval of the Bordeaux mixture aqueous solution preventing disease and pest infringement of sprinkling in 9 days, find to have to go mouldy and change colour during cuttage rooting and do
Withered phenomenon is removed in time；
B, after cuttage rooting, the Gibberellins solution for being 1000ppm with concentration sprays cuttings, interval concentration after 2 days
Cuttings is sprayed for 1000ppm indole -3-butyric acid solution, until the gemma in cuttings grows sprouting, then changing spraying concentration is
500ppm 6-benzyladenine solution accelerates the growth of sprouting；
Wherein, spray is defined so that cuttings is squirted；
C, the preventing and treating for carrying out rich water quality management and pest and disease damage, can obtain intact hybridization paper mulberry cuttage seeding in 2 months.
Comparative example 1：
Do not use the matrix of the application to be cultivated, but use the charcoal of Shuyang County Xinhe River town little Mo flower plants and nursery stocks
Block fertile soil nutrient matrix is cultivated, and other processing are same as Example 3.
Comparative example 2：
The slurry of taking root of the application is not used, but is directly configured to take root slurry to hybridization structure using root-inducing powder+yellow mud
Tree cuttage seedling inclined-plane is handled, and other processing are same as Example 3.
Comparative example 3：
Nursery is not carried out using container for plant growth, the nursery directly on seedling culture frame, other processing are same as Example 3.
Embodiment 1-4 and comparative example 1-3 is subjected to experimental plot cultivation on rivers and ponds Dahua County greenhouse farm, and to growth feelings
Condition is recorded, and records particular situation such as following table：
Table 1
From above-mentioned data, the cuttage seeding obtained by the application matrix and method plantation is suitable for the ring of growth
Border, survival rate is higher.
The obtained paper mulberry cuttage seeding of embodiment 1-4 and comparative example 1-3 is divided into 7 each groups, each three groups of component, each
10 nursery stocks of group, guest Wuxuan Mao Shan, Nanning are planted in by the nursery stock of each embodiment and three groups of comparative example respectively
Wuming mouse mountain and statistics after the field planting half a year of rivers and ponds bigization cockchafer mountain, are shown in Table 2：
Table 2
| Project | Guest Wuxuan survival tree | Nanning Wuming survival tree | Rivers and ponds bigization survival tree |
| Embodiment 1 | 10 | 9 | 10 |
| Embodiment 2 | 9 | 9 | 9 |
| Embodiment 3 | 10 | 9 | 10 |
| Embodiment 4 | 10 | 9 | 10 |
| Comparative example 1 | 8 | 7 | 8 |
| Comparative example 2 | 7 | 7 | 8 |
| Comparative example 3 | 6 | 6 | 5 |
As seen from the above table, the paper mulberry survival rate come out using the present processes and substrate seedling is had compared with prior art
Certain advantage.
The obtained slow release fertilizers of embodiment 1-4 are placed in the environment of certain humidity, retrieval slow release fertilizer starts dissolving
Discharge the time of fertility
Each three groups of embodiment point, is humidity 50%, humidity 65%, humidity 75% respectively per three moist gradients of component
Detected, testing result is shown in Table 3：
Table 3
As shown in Table 2, the time that the slow release fertilizer that prepared by the application discharges fertilizer under certain condition is different, can basis
Different demand conditions cause by ambient humidity, obtain the time of required fertility matched with plant growth.
The invention is not limited in foregoing embodiment.The present invention, which is expanded to, any in this manual to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (7)
1. a kind of method for culturing seedlings for hybridizing paper mulberry, it is characterised in that：Including matrix sterilization, the selection of cuttage branch and processing, skewer
Insert and cuttage final-period management；
(1) matrix is sterilized：It will be fitted into after matrix in container for plant growth, be dense with quality again after 75% alcohol disinfecting with mass concentration
Spend and matrix is carried out disinfection for 3% limewash, it is standby after then being covered 5 days with film；
Wherein, matrix is mixed by the raw material of following parts by weight：10-15 parts of slow release fertilizer, 8-12 parts of activating phosphorite powder,
Middle sand 5-10 parts, 5-10 parts of distillers' grains, 3-8 parts of fertile soil, 3-5 parts of diatomite, 3-8 parts of earthworm powder, 3-5 parts of rock wool, chitosan 3-5
Part, expanded chicken manure 3-5 parts；
(2) selection and processing of cuttage branch, specifically include following processing：
1. the branch for selecting stalwartness is material；
2. the branch chosen is chipped away from hybridization paper mulberry with knife, 2-3 bud point then has been left and taken into branch progress amputation.
Then carry out inclined-plane trimming to the bottom of branch, then by beveled ends trim with bacterium spirit solution or mass concentration be 25-
35% potassium permanganate dilutes 200-500 times and soaked 8-10 minutes, is rinsed after taking-up with clear water, obtains cuttage branch stand-by；
3. sugar is added in ABT takes root the solution that powder content is 1-5mg/L, stirring and dissolving is subsequently added into foregoing hormotone molten
Liquid, is subsequently added into acid loess and stirs into slurry of taking root, and will take root slurry natural cooling after high-temperature sterilization 18-25 minutes, stand-by；
Wherein, sugared addition accounts for the 2% of solution weight, and the addition of hormotone solution accounts for the 1% of solution weight；
Wherein, hormotone solution is heteroauxin, 6- benzyls aminoadenine, methyl α-naphthyl acetate according to 10-15:2-3:3-5 weight
Obtained than mixed configuration；
4. the beveled ends point of the cuttage branch obtained after 2. step is handled dips in slurry of taking root；
(3) cuttage：After being handled by step (1), the centre of the matrix in container for plant growth holds aperture successfully for 3-5 centimetres, depth
For 3-5 centimetres of matrix cuttage cave, the central axis in the matrix cuttage cave is horizontal by 35 ° -45 °, by cuttage branch
The beveled ends cuttage that point dips in slurry of taking root enters matrix cuttage cave, then fills up cave, then root water of drenching using river sand；
(4) cuttage final-period management：
A, seedling in greenhouse is carried out to the cuttings after cuttage, the shading rate of greenhouse is 75% during cuttage rooting, keeps matrix
Moisture content is 60%-70%, and to foliage-spray water, keeps blade face to moisten 4 hours/day, continuous wetness 7-15 days treats blade face
After rejuvenating, remove the flower the more balance fertilizer for once diluting 800-1000 times is sprayed after sheltering from heat or light completely, be spaced 7-10 days and spray afterwards
Apply once, while being encroached at interval of the Bordeaux mixture aqueous solution preventing disease and pest of sprinkling in 7-10 days, found during cuttage rooting
There are fallen leaves, the phenomenon dried up of going mouldy or/and change colour is removed in time；
B, after cuttage rooting, the Gibberellins solution for being 1000ppm with concentration sprays cuttings, interval after 2-3 days concentration be
1000ppm indole -3-butyric acid solution sprays cuttings, until the gemma in cuttings grows sprouting, then changing spraying concentration is
500ppm 6-benzyladenine solution accelerates the growth of sprouting；
Wherein, spray is defined so that cuttings is squirted；
C, the preventing and treating for carrying out rich water quality management and pest and disease damage, can obtain intact hybridization paper mulberry cuttage seeding in 1-2 months.
2. it is according to claim 1 hybridization paper mulberry method for culturing seedlings, it is characterised in that it is described step 3. in, the height
Temperature sterilization is carried out disinfection under the conditions of temperature is 90-100 DEG C.
3. the method for culturing seedlings of hybridization paper mulberry according to claim 1, it is characterised in that the slow release fertilizer is by following weight
Part raw material is made：3-5 parts of lapping, 10-20 parts of effective fertilizer composition, 4-5 parts of auxiliary material；
Wherein lapping is obtained by mixing by following raw material：5-8 parts of sodium alginate oligosaccharide, 1-3 parts of epoxy resin, xanthan
0.5-1.5 parts of glue；
The effective fertilizer composition is made up of following raw material：Trace element, urea, ammonium chloride, tortoise shell, pig testis, white granulated sugar,
Cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek, Gleditsia officinalis deadwood and cellulase；
Include boron fertilizer, cobalt fertilizer, zinc fertilizer, copper fertilizer, molydbenum fertilizer, manganese fertilizer, iron fertilizer in parts by weight；
The auxiliary material includes N- NBPTs and 3- methylpyrazoles.
4. the method for culturing seedlings of hybridization paper mulberry according to claim 1, it is characterised in that the slow release fertilizer is by following
What method was prepared：
A, by tortoise shell, pig testis crush mix with white granulated sugar holding humidity be 65%, fermentation process 5-8 days, obtain albumen send out
Ferment thing；
B, cocoanut tree skin, banyan deadwood, lotus bar, vicia amoena rattan, wild leek and Gleditsia officinalis deadwood be crushed to particle diameter 1-2cm,
Then mixed with cellulase, fermentation process 2-3 days obtains fiber fermentation thing；
C, protein fermentation thing, fiber fermentation thing and trace element, urea, ammonium chloride is mixed to get effective fertilizer composition；
D, N- NBPTs, 3- methylpyrazoles and effective fertilizer composition be mixed to get first composition fertilizer；
E, first composition fertilizer is sprayed to aqueous solution stirring moistening be defined to not water outlet is held, be then placed in the parcel constantly rotated
In machine, then lapping equably sprayed into by the nozzle on packing machine, until the granular fertilizer that particle diameter is 2-3mm is obtained,
Then processing is dried, packs and gets product.
5. the method for culturing seedlings of hybridization paper mulberry according to claim 1, it is characterised in that the container for plant growth is wide opening cup；
The wide opening cup is bottomless penetrating shape；The cup of wide opening cup includes at least two layers, and outermost layer is that film, innermost layer are ox-hide
Paper.
6. it is according to claim 1 hybridization paper mulberry method for culturing seedlings, it is characterised in that the activating phosphorite powder be specifically by
What following methods were prepared：The phosphorus ore slag for choosing Phosphorus Mineral Area is put into broth bouillon, Shaking culture 3 hours at 38 DEG C,
Bacterium night progress plate streak is cultivated on nutrient medium again, the condition of culture is that temperature is 37 DEG C, 5-6 hours,
Then choose the dimmed bacterium colony of growing way presentation and be configured to bacterium solution, it is stand-by；By phosphorus content 250-300mg/kg phosphorus ore crushing, ball
Mill, crosses 100 mesh sieves, is then added in stand-by bacterium solution, and Shaking culture is carried out 2 hours under the conditions of temperature is 38 DEG C, you can
Obtain activating phosphorite powder.
7. it is according to claim 1 hybridization paper mulberry method for culturing seedlings, it is characterised in that the expanded chicken manure be specifically by with
Lower section method is prepared：It is 50 DEG C of spontaneous fermentation 5-8 hour that chicken manure, which is built heap to keep internal temperature, and chicken manure then is opened into oneself
So cooling, is then heated to thermal cracking 2-3S at 800-900 DEG C, you can obtain expanded chicken manure in boiler.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN114504669A (en) * | 2021-12-27 | 2022-05-17 | 正大农业科技(浙江)有限公司 | Safe disinfection equipment and disinfection method for soilless culture substrate |
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