CN110537488A - Detection method for breeding of cinnamomum longepaniculatum improved variety - Google Patents
Detection method for breeding of cinnamomum longepaniculatum improved variety Download PDFInfo
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- CN110537488A CN110537488A CN201910639041.8A CN201910639041A CN110537488A CN 110537488 A CN110537488 A CN 110537488A CN 201910639041 A CN201910639041 A CN 201910639041A CN 110537488 A CN110537488 A CN 110537488A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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Abstract
The invention relates to the technical field of monkeytree fruit seed selection, and discloses a detection method for breeding improved monkeytree fruit seeds, which comprises the following steps: s1: selecting mature seeds with excellent growth vigor, picking the seeds, rotting, washing with water to remove seed coats, spreading the cleaned seeds in shade for one day, and storing the seeds in a sand mixing manner until the next year; s2: putting the substrate into a seedling culture container, disinfecting the substrate by using 50% alcohol and then using 3% lime water, covering the substrate by using a film for 5 days, and then sowing the monkeytree root and camphor seeds into the substrate; s3: after the seedlings come out of the earth, the terminal buds are treated by adopting an artificial mutagenesis method to obtain a mutagenesis material, and after the mutagenesis material is cultured for 15 days, variant branches are selected to extract chromosomes of the variant branches. The detection method for the improved variety breeding of the monkeynut tree solves the problem that the quality of the planted monkeynut tree is uneven and the planting income is influenced because most monkeynut tree seeds are directly planted at present and no improved variety breeding step is carried out.
Description
Technical Field
The invention relates to the technical field of monkeyhead camphor seed selection, in particular to a detection method for fine monkeyhead camphor seed breeding.
Background
monkeyau camphor (Cinnamomum bodinieri Levl.) is named separately: camphor, bergamot tree, nanmu, monkey-wood, camphor tree, arbor, up to 16 m. Red brown bark; the small branch is cylindrical and dark purple, and the tail section is provided with angular edges; . Leaf intergrowth, thick paper, oval or elliptical oval, 8-17 cm long and 3-10 cm wide. Is a plant which grows in a mountain wild forest and has the functions of dispelling wind and removing dampness; warming middle energizer to dispel cold; move qi and alleviate pain. Mainly treating wind-cold type common cold; rheumatic arthralgia; vomiting and diarrhea abdominal pain, abdominal mass; hernia pain, etc. Because the monkeytree fruit has huge medicinal value, people cultivate the monkeytree fruit more and more extensively, the monkeytree fruit seeds are mostly directly planted at present, and fine breed breeding steps are not carried out, so that the planted monkeytree fruit has uneven quality, and the planting income is influenced.
Disclosure of Invention
technical problem to be solved
Aiming at the defects of the prior art, the invention provides a detection method for breeding improved monkeytree fruit tree seeds, which has the advantage of improving the planting yield and solves the problems that the quality of the planted monkeytree fruit tree seeds is uneven and the planting yield is influenced because most monkeytree fruit tree seeds are directly planted at present and the improved variety breeding step is not carried out.
(II) technical scheme
In order to realize the purpose of improving the planting income, the invention provides the following technical scheme: a detection method for breeding improved monkeytree bark seeds comprises the following steps:
S1: selecting mature seeds with excellent growth vigor, picking the seeds, rotting, washing with water to remove seed coats, spreading the cleaned seeds in shade for one day, and storing the seeds in a sand mixing manner until the next year;
S2: putting the substrate into a seedling culture container, disinfecting the substrate by using 50% alcohol and then using 3% lime water, covering the substrate by using a film for 5 days, and then sowing the monkeytree root and camphor seeds into the substrate;
s3: after the seedlings come out of the earth, processing terminal buds by adopting an artificial mutagenesis method to obtain a mutagenesis material, culturing the mutagenesis material for 15 days, selecting variant branches, and extracting chromosomes of the variant branches;
s4: detecting the extracted chromosome by using a PCR technology, and searching a mutation site of the chromosome;
S5: cutting off redundant branch buds when the seedlings grow to a fixed main rod of 10 cm, reserving a thick branch, transplanting the branch, and recording the transplanted seedlings in real time;
S6: after the seedlings grow up, the best plants are selected, the corresponding chromosome detection results are selected, mutation sites of the plants are confirmed, a foundation is provided for analyzing the relation between the mutation sites and the goodness, and reference and basis can be provided for the screened cinnamomum longepaniculatum fine breeds according to the detection results.
Preferably, in the step S1, the seed collecting time is 10 months per year, and the seeding time in the step S2 is 3 months per year.
Preferably, in the step S2, the substrate is made of 10 parts of nitrogen fertilizer, 5 parts of plant ash, 3 parts of humus, 6 parts of earthworm powder and 3 parts of chicken manure.
preferably, in the step S2, before the cinnamomum kanahirai hance seeds are sown into the substrate, germination is performed on the cinnamomum kanahirai hance seeds, and the germination method is a water bath germination method or a film-wrapped germination method.
preferably, in the step S3, the artificial mutagenesis method includes a physical mutagenesis method and a chemical mutagenesis method, the physical mutagenesis method is one of X-ray, gamma-ray, ultraviolet ray and laser, and the chemical mutagenesis method is one of nitrous acid and diethyl sulfate.
Preferably, in the step S2, when the cinnamomum kanahirai hay bunge seeds are sown in the substrate, the row spacing of the seeds is sown in a row of 20cm, and the seeds are covered with rice straws after being sown.
Preferably, the cinnamomum kanahirai hay bunge seeds are marked in the whole process, and the growth log of each marked cinnamomum kanahirai hay bunge seed is recorded.
Preferably, the water bath germination accelerating method is to soak seeds with warm water at 50 ℃, and the seeds are soaked in the warm water again for 3 times by changing the water at 50 ℃ after the warm water is cooled.
preferably, the film-coated germination accelerating method is to coat seeds mixed with river sand with a film, sun-dry the seeds in the sun, turn over the seeds 3 times a day, keep the seeds moist, and sow the seeds until a small amount of seeds begin to germinate.
(III) advantageous effects
Compared with the prior art, the invention provides a detection method for breeding improved monkeytree fruit seeds, which has the following beneficial effects:
the detection method for the breeding of the improved monkeytree bark seeds comprises the steps of selecting excellent primary monkeytree bark seeds, carrying out mutagenesis on the seeds, selecting the mutated improved monkeytree bark seeds, detecting extracted chromosomes according to a PCR technology, determining mutation sites of the extracted chromosomes, providing a basis for analyzing the relationship between the mutation sites and the excellence, and providing reference and basis for the screened improved monkeytree bark seeds according to detection results, so that the improved monkeytree bark seeds are finally obtained according to the results, the growth performance of disease resistance of the improved monkeytree bark seeds is improved, and the planting income is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
a detection method for breeding improved monkeytree bark seeds comprises the following steps:
S1: selecting mature seeds with excellent growth vigor, picking the seeds, rotting, washing with water to remove seed coats, spreading the cleaned seeds in shade for one day, and storing the seeds in a sand mixing manner until the next year;
S2: putting the substrate into a seedling culture container, disinfecting the substrate by using 50% alcohol and then using 3% lime water, covering the substrate by using a film for 5 days, and then sowing the monkeytree root and camphor seeds into the substrate;
S3: after the seedlings come out of the earth, processing terminal buds by adopting an artificial mutagenesis method to obtain a mutagenesis material, culturing the mutagenesis material for 15 days, selecting variant branches, and extracting chromosomes of the variant branches;
S4: detecting the extracted chromosome by using a PCR technology, and searching a mutation site of the chromosome;
S5: cutting off redundant branch buds when the seedlings grow to a fixed main rod of 10 cm, reserving a thick branch, transplanting the branch, and recording the transplanted seedlings in real time;
S6: after the seedlings grow up, the best plants are selected, the corresponding chromosome detection results are selected, mutation sites of the plants are confirmed, a foundation is provided for analyzing the relation between the mutation sites and the goodness, and reference and basis can be provided for the screened cinnamomum longepaniculatum fine breeds according to the detection results.
The method comprises the steps of selecting excellent native monkeytree fruit seeds, carrying out mutagenesis on the seeds, selecting improved monkeytree fruit seeds after mutagenesis, detecting extracted chromosomes according to a PCR technology, determining mutation sites of the seeds, providing a basis for analyzing the relation between the mutation sites and the excellence, and providing reference and basis for the screened improved monkeytree fruit seeds according to detection results, so that the improved monkeytree fruit seeds are finally obtained according to the results, the growth performance of disease resistance of the seeds is improved, and the planting income is improved.
in the step S1, the seed collecting time is 10 months per year, and the seeding time in the step S2 is 3 months per year.
in the step S2, the matrix is made of 10 parts of nitrogen fertilizer, 5 parts of plant ash, 3 parts of humus, 6 parts of earthworm powder and 3 parts of chicken manure.
and S2, before the cinnamomum kanehirae presl seeds are sowed into the substrate, accelerating germination by a water bath accelerating germination method or a film-wrapped accelerating germination method.
In the step S3, the artificial mutagenesis method includes a physical mutagenesis method and a chemical mutagenesis method, the physical mutagenesis method is X-ray, and the chemical mutagenesis method is diethyl sulfate.
And S2, when the cinnamomum kanehirae dunn seeds are sowed into the matrix, sowing row spacing is 20cm, covering the straw with soil after sowing, and keeping the surface soil of the matrix moist to facilitate germination of the seeds.
The monkeytree fruit seeds are marked in the whole process, and the growth log of each marked monkeytree fruit seed is recorded.
A water-bath germination accelerating method for promoting germination of seeds features that the seeds are immersed in warm water at 50 deg.C, and the seeds are immersed in the warm water again at 50 deg.C for 3 times to make the seeds germinate in advance for 10 days.
A film-coated germination accelerating method for seeds with river sand includes such steps as coating the seeds with film, sunning, turning over 3 times each day, moistening until a small amount of seeds begin to germinate, and sowing again for 10 days.
to sum up, the detection method for the breeding of the improved monkeytree bark seeds selects excellent primary monkeytree bark seeds, carries out mutagenesis on the seeds, picks out the mutated improved monkeytree bark seeds, detects the extracted chromosomes according to the PCR technology, ensures mutation sites of the seeds, provides a basis for analyzing the relation between the mutation sites and the advantages, and can provide reference and basis for the screened improved monkeytree bark seeds according to detection results, thereby finally obtaining the improved monkeytree bark seeds according to the results, improving the growth performance of the disease resistance of the seeds and further improving the planting income.
It is to be noted that the term "comprises," "comprising," or any other variation thereof is intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A detection method for breeding of an improved variety of a cinnamomum kanahirai dunn is characterized by comprising the following steps:
S1: selecting mature seeds with excellent growth vigor, picking the seeds, rotting, washing with water to remove seed coats, spreading the cleaned seeds in shade for one day, and storing the seeds in a sand mixing manner until the next year;
S2: putting the substrate into a seedling culture container, disinfecting the substrate by using 50% alcohol and then using 3% lime water, covering the substrate by using a film for 5 days, and then sowing the monkeytree root and camphor seeds into the substrate;
S3: after the seedlings come out of the earth, processing terminal buds by adopting an artificial mutagenesis method to obtain a mutagenesis material, culturing the mutagenesis material for 15 days, selecting variant branches, and extracting chromosomes of the variant branches;
S4: detecting the extracted chromosome by using a PCR technology, and searching a mutation site of the chromosome;
S5: cutting off redundant branch buds when the seedlings grow to a fixed main rod of 10 cm, reserving a thick branch, transplanting the branch, and recording the transplanted seedlings in real time;
S6: after the seedlings grow up, the best plants are selected, the corresponding chromosome detection results are selected, mutation sites of the plants are confirmed, a foundation is provided for analyzing the relation between the mutation sites and the goodness, and reference and basis can be provided for the screened cinnamomum longepaniculatum fine breeds according to the detection results.
2. The detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 1, wherein the detection method comprises the following steps: in the step S1, the seed collecting time is 10 months per year, and the seeding time in the step S2 is 3 months per year.
3. The detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 1, wherein the detection method comprises the following steps: in the step S2, the matrix is made of 10 parts of nitrogen fertilizer, 5 parts of plant ash, 3 parts of humus, 6 parts of earthworm powder and 3 parts of chicken manure.
4. the detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 1, wherein the detection method comprises the following steps: and in the step S2, before the cinnamomum kanehirae presl seeds are sowed into the substrate, carrying out pregermination treatment on the cinnamomum kanehirae presl seeds, wherein the pregermination method is a water bath pregermination method or a film-wrapped pregermination method.
5. The detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 1, wherein the detection method comprises the following steps: in the step S3, the artificial mutagenesis method includes a physical mutagenesis method and a chemical mutagenesis method, the physical mutagenesis method is one of X-ray, gamma-ray, ultraviolet ray and laser, and the chemical mutagenesis method is one of nitrous acid and diethyl sulfate.
6. the detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 1, wherein the detection method comprises the following steps: and in the step S2, when the cinnamomum kanehirae dunn seeds are sowed into the matrix, the row spacing of the seeds is sowed in a row manner by 20cm, and the straws are covered with soil after sowing.
7. The detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 1, wherein the detection method comprises the following steps: and marking the monkeytree fruit seeds in the whole process, and recording the growth log of each marked monkeytree fruit seed.
8. The detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 4, wherein the detection method comprises the following steps: the water bath germination accelerating method specifically comprises the steps of soaking seeds in warm water at 50 ℃, and repeatedly soaking the seeds in water at 50 ℃ for 3 times after the warm water is cooled.
9. the detection method for the breeding of the cinnamomum longepaniculatum fine variety according to claim 4, wherein the detection method comprises the following steps: the film-wrapped germination accelerating method specifically comprises the steps of wrapping seeds mixed with river sand with a film, drying the seeds in the sun, turning over the seeds 3 times every day, keeping the seeds moist, and sowing the seeds when a small amount of seeds begin to germinate.
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Citations (5)
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| CN106941958A (en) * | 2017-02-28 | 2017-07-14 | 河池乐康生态农业科技有限公司 | A kind of method for culturing seedlings for hybridizing paper mulberry |
| CN107251724A (en) * | 2016-09-23 | 2017-10-17 | 雷学军 | Develop the method that new weather economy realizes net zero carbon emission in the whole world |
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2019
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| JPH10191776A (en) * | 1996-12-28 | 1998-07-28 | Gunze Ltd | Vegetative propagation method of camphor-wood by cuttage |
| WO2010002276A1 (en) * | 2008-06-30 | 2010-01-07 | New Zealand Forest Research Institute Limited | Compositions and methods for improving trees |
| CN105918114A (en) * | 2016-01-05 | 2016-09-07 | 湖南省核农学与航天育种研究所 | Method for improving screening efficiency of target traits in plant radiation mutation breeding |
| CN107251724A (en) * | 2016-09-23 | 2017-10-17 | 雷学军 | Develop the method that new weather economy realizes net zero carbon emission in the whole world |
| CN106941958A (en) * | 2017-02-28 | 2017-07-14 | 河池乐康生态农业科技有限公司 | A kind of method for culturing seedlings for hybridizing paper mulberry |
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Application publication date: 20191206 |