CN106072665A - A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof - Google Patents
A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof Download PDFInfo
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- CN106072665A CN106072665A CN201610488413.8A CN201610488413A CN106072665A CN 106072665 A CN106072665 A CN 106072665A CN 201610488413 A CN201610488413 A CN 201610488413A CN 106072665 A CN106072665 A CN 106072665A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
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Abstract
The invention discloses a kind of person in middle and old age with aminoacid calcium-replenishing marine protein health product and preparation method thereof, scientific compatibility multiple marine protein raw material, fermentable and multistep cellulase hydrolysis technology is utilized to obtain rich in amino acid whose marine protein health promoting product, on the basis of realizing the high-valued exploitation of marine protein, cultivate novel sea economy, meet middle-aged and elderly people nourishing healthy demand.The marine health product color that the present invention uses " ultrasound-assisted enzymolysis+aerobic fermentation process+secondary enzymolysis " to arrive is brilliant white, powder, free from admixture, without bitterness, total protein content >=98%, gross protein middle-molecular-weihydroxyethyl peptide content >=90% below 1000 dalton.The inventive method is with short production cycle, low cost, does not produce any poisonous and harmful substance, and products obtained therefrom safety is high, functional activity absorption good, balanced in nutrition, easy to digest；Having and significantly replenish the calcium, improve the effects such as middle-aged and elderly people immunity of organisms, market application has a extensive future.
Description
Technical field
The present invention relates to a kind of person in middle and old age with aminoacid calcium-replenishing marine protein health product and preparation method thereof, belong to health care
Food processing field.
Background technology
Protein is the main matter constituting human tissue organ, plays highly important work in human life activity
With, it is to maintain and repair necessary to body and cell growth, and it not only affects the growth of body tissue, also participates in hormone
All many-sides such as the generation of generation, the maintenance of immunologic function, other nutrient substance and the transhipment of oxygen and hemoglobin, because of
This, protein is to ensure that one of healthy important nutrient.
21 century is numerical ocean model, is the century of Sustainable Development of Marine Economy.In the ocean taking up an area the ball gross area 71%
Live about 400,000 kinds of biologies, take up an area the 80% of ball living species sum.Someone estimates, ocean the most about produces 1350 × 108
Ton organic carbon.In the case of not destroying ecological balance, ocean can provide 30 × 10 every year8Ton aquatic products, enough 30,000,000,000 people's foods
With.Also someone calculates, ocean provides the ability of food to the mankind, is equivalent to land, the whole world the provided food of cultivated area
1000 times.China not only has wide land, and has unbroken coastline, total up to more than 1.8 ten thousand kilometer, is in the world
One of country that coastline is the longest, marine protein aboundresources.At present, the amount of fishing of marine fish, shrimp, shellfish etc. oneself reach 93,000,000
t/a.These protein resources in addition to part is directly the most edible, most of protein resource of low value and the leftover bits and pieces of processing of aquatic products or
By squeezing, drying and be processed into fish flour, or discard, value-added content of product is the lowest.The waste water of fish flour processing is directly discharged into coastal waters
Waters, causes the severe contamination of offshore waters.Therefore, the marine protein to low value carries out high-valued, resource, ecology
Utilize, be that current marine biotechnology is badly in need of the content that conducts a research.Wherein, use fermentation engineering and enzyme engineering technology, be out
Send out the important channel utilizing marine protein resource.Protein is after protease hydrolyzed, and it is low that its peptide prod has molecular weight, breast
Change heat energy power effective, suitable to increase, be easily absorbed by organisms and the advantage such as new biological activity, make them can be applied to many
Field, especially has broad application prospects in field of health care food.
Shortage and the loss of calcium can generally occur after people in middle age, old during therefore calcium nutrition and effective calcium-supplement are
The one big health problem of year people, the mid-aged population that China's needs are replenished the calcium accounts for 50%.Shortage and the loss of calcium are usually associated with bone
Meeting the loss of middle collagen protein and shortage, therefore, replenishing the calcium and be necessary for replenishing collagen in bone road, collagen peptide is extremely easy to absorption of human body
Utilizing, be human body calcium absorption and effective medium of bone road calcium deposit, deep-sea fish skin collagen peptide is the purest current, safety equally
" green " ossein nutrient.Therefore, exploitation middle-aged and elderly people calcium-replenishing marine protein health product can meet middle-aged and elderly people guarantor
Strong demand, again can high-valued exploitation marine protein resource, cultivate novel sea economy.But marine protein product mostly is at present
Directly drying after protein purification and pulverize or prepare through simple enzymolysis process, in product, amino acid content is on the low side, not only affects sea
The performance of ocean protein function activity, and have impact on the palatability of marine protein health promoting product.
Summary of the invention
The present invention provides a kind of person in middle and old age by aminoacid calcium-replenishing marine protein health product and preparation method thereof, scientific compatibility
Multiple marine protein raw material, utilizes fermentable and multistep cellulase hydrolysis technology to obtain and keeps healthy rich in amino acid whose marine protein
Product, cultivates novel sea economy on the basis of realizing the high-valued exploitation of marine protein, meets middle-aged and elderly people nutrition and protects
Strong demand.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The present invention provides a kind of person in middle and old age with aminoacid calcium-replenishing marine protein health product and preparation method thereof, it is characterised in that tool
Body comprises the following steps:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
It is (2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%,
Regulation pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of guarantors
Temperature 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: add relative to marine protein mass ratio 3～5% molasses, 1～2% in the marine protein after hydrolysis
Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2～3%, 1～2% inoculation ratio
Example is seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: add relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 albumen
Enzyme, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) it is spray-dried: concentrated solution is carried out spray drying and obtains product middle-aged and elderly people calcium-replenishing marine protein health product.
The present invention provides a kind of person in middle and old age with aminoacid calcium-replenishing marine protein health product and preparation method thereof, described ocean
Albumen is Oyster Protein, deep-sea cod collagen protein powder, scallop egg albumen powder and the mixture of shark cartilage powder, and its quality group becomes
Oyster Protein 20～30 parts, deep-sea cod collagen protein powder 30～40 parts, scallop protein 20～30 parts, shark cartilage powder 10～20
Part.
The present invention provides a kind of person in middle and old age with aminoacid calcium-replenishing marine protein health product and preparation method thereof, the sea obtained
Ocean albumen health-care product color and luster is brilliant white, powder, free from admixture, without bitterness, connected fermentable effect and makes in product not
Contain only marine protein, and the most certain tropina, total protein content >=98% in product, the most water-soluble after measured
Property protein content >=99%；Gross protein middle-molecular-weihydroxyethyl peptide content >=90% below 1000 dalton；Amino acid content >=
70%, amino acid content equalizes, and the particularly comparision contents of essential amino acids is high, and its ratio is apparently higher than WHO/FAO standard
(35.38%).The reducing power of marine protein health product reaches 0.507, and ABTS radical scavenging activity reaches 1.96mmol/L
Trolox.The effect of supplemented calcium more existing report level utilizing zoopery to measure marine protein health product exceeds 38.44%；Have
Significantly replenishing the calcium, improve the effects such as middle-aged and elderly people immunity of organisms, market application has a extensive future；And test result indicate that and change
Become marine protein raw material composition or production technology can significantly reduce marine protein health product effect (P < 0.01).
Detailed description of the invention
Embodiment 1:
A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof, and concrete preparation process is as follows:
(1) dispensing: by Oyster Protein 30 parts, deep-sea cod collagen protein powder 30 parts, 30 parts of scallop albumen, shark cartilage powder 10 parts
Accurate feed proportioning；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 2%, 1%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulations
3h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 1h；
(7) preparation culture medium: add relative to marine protein mass ratio 5% molasses, 2% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: Candida utilis, saccharomyces cerevisiae are inoculated by relative marine protein mass ratio 3%, 2% inoculative proportion
To culture medium, cultivate 12h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 2%SM98011 protease, 3%
Flavor protease, enzymolysis 2h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product middle-aged and elderly people calcium-replenishing marine protein health product.
Embodiment 2:
A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof, and concrete preparation process is as follows:
(1) dispensing: by Oyster Protein 20 parts, deep-sea cod collagen protein powder 40 parts, scallop protein 20 part, shark cartilage powder 20 parts
Accurate feed proportioning；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1%, 0.5%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 60min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulations
4h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 2h；
(7) preparation culture medium: add relative to marine protein mass ratio 3% molasses, 1% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: Candida utilis, saccharomyces cerevisiae are inoculated by relative marine protein mass ratio 2%, 1% inoculative proportion
To culture medium, cultivate 24h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 1%SM98011 protease, 2%
Flavor protease, enzymolysis 3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product middle-aged and elderly people calcium-replenishing marine protein health product.
Embodiment 3:
A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof, and concrete preparation process is as follows:
(1) dispensing: by Oyster Protein 25 parts, deep-sea cod collagen protein powder 35 parts, scallop protein 25 part, shark cartilage powder 15 parts
Accurate feed proportioning；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1.5%, 0.8%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 45min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2.5 ‰, regulates pH6.0～7.0,60 DEG C of insulations
3.5h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 4 ‰, controls 40 DEG C of hydrolysis 1.5h；
(7) preparation culture medium: add relative to marine protein mass ratio 4% molasses, 1.5% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.35% ammonium sulfate, 0.15% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2.5%, 1.5% inoculative proportion
It is seeded to culture medium, cultivates 18h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 1.5%SM98011 protease,
2.5% flavor protease, enzymolysis 2.5h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product middle-aged and elderly people calcium-replenishing marine protein health product.
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to aforementioned reality
Execute example to be described in detail by invention, but for the person of ordinary skill of the art, still can be to aforementioned reality
Execute the technical scheme described in example to modify, or wherein portion of techniques feature is carried out equivalent；And these are revised
Or replace, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (2)
1. person in middle and old age are by the preparation method of aminoacid calcium-replenishing marine protein health product, it is characterised in that specifically include following
Step:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
It is (2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%,
Regulation pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of guarantors
Temperature 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: add relative to marine protein mass ratio 3～5% molasses, 1～2% in the marine protein after hydrolysis
Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2～3%, 1～2% inoculation ratio
Example is seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: add relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 albumen
Enzyme, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) be spray-dried: concentrated solution is carried out spray drying obtain product person in middle and old age with aminoacid calcium-replenishing marine protein keep healthy
Product.
A kind of person in middle and old age the most according to claim 1 by the preparation method of aminoacid calcium-replenishing marine protein health product, its
It is characterised by that described marine protein is the mixed of shark protein, deep-sea cod collagen protein powder, scallop egg albumen powder and shark cartilage powder
Compound, its quality group becomes shark protein 20～30 parts, deep-sea cod collagen protein powder 30～40 parts, scallop protein 20～30
Part, shark cartilage powder 10～20 parts.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110637998A (en) * | 2019-11-01 | 2020-01-03 | 青岛浩大生物科技工程有限责任公司 | Composite fermentation type marine functional product |
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| CN102133231A (en) * | 2011-03-23 | 2011-07-27 | 济南康麦国际贸易有限公司 | Product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage and preparation method thereof |
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| CN110637998A (en) * | 2019-11-01 | 2020-01-03 | 青岛浩大生物科技工程有限责任公司 | Composite fermentation type marine functional product |
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