CN106072548A - A kind of rich in amino acid whose infant marine protein health product and preparation method thereof - Google Patents
A kind of rich in amino acid whose infant marine protein health product and preparation method thereof Download PDFInfo
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- CN106072548A CN106072548A CN201610485958.3A CN201610485958A CN106072548A CN 106072548 A CN106072548 A CN 106072548A CN 201610485958 A CN201610485958 A CN 201610485958A CN 106072548 A CN106072548 A CN 106072548A
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 99
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 99
- 230000036541 health Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 title abstract description 13
- 230000007062 hydrolysis Effects 0.000 claims abstract description 17
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 17
- 230000000694 effects Effects 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 238000002604 ultrasonography Methods 0.000 claims abstract description 11
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 5
- 239000000047 product Substances 0.000 claims description 36
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 15
- 239000004365 Protease Substances 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 11
- 239000012141 concentrate Substances 0.000 claims description 10
- 238000010612 desalination reaction Methods 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
- 235000019419 proteases Nutrition 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 241000965254 Apostichopus japonicus Species 0.000 claims description 7
- 101100421450 Drosophila melanogaster Shark gene Proteins 0.000 claims description 7
- 241000239366 Euphausiacea Species 0.000 claims description 7
- 102000004407 Lactalbumin Human genes 0.000 claims description 7
- 108090000942 Lactalbumin Proteins 0.000 claims description 7
- 239000004382 Amylase Substances 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 108091005658 Basic proteases Proteins 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 5
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 5
- 239000000796 flavoring agent Substances 0.000 claims description 5
- 235000019634 flavors Nutrition 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 235000013379 molasses Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000012010 growth Effects 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 2
- 108010059892 Cellulase Proteins 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 239000011575 calcium Substances 0.000 abstract description 2
- 229910052791 calcium Inorganic materials 0.000 abstract description 2
- 229940106157 cellulase Drugs 0.000 abstract description 2
- 230000005714 functional activity Effects 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 231100000614 poison Toxicity 0.000 abstract 1
- 230000007096 poisonous effect Effects 0.000 abstract 1
- 235000013305 food Nutrition 0.000 description 5
- 238000009413 insulation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000002002 slurry Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 235000013360 fish flour Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- -1 because of This Proteins 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
- A23J3/08—Dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/343—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of rich in amino acid whose infant marine protein health product and preparation method thereof, scientific compatibility multiple marine protein raw material, fermentable and multistep cellulase hydrolysis technology is utilized to obtain rich in amino acid whose marine protein health promoting product, on the basis of realizing the high-valued exploitation of marine protein, cultivate novel sea economy, meet infant growth health care demand.The marine health product color that the present invention uses " ultrasound-assisted enzymolysis+aerobic fermentation process+secondary enzymolysis " to arrive is brilliant white, powder, free from admixture, without bitterness, total protein content >=98%, gross protein middle-molecular-weihydroxyethyl peptide content >=90% below 1000 dalton.The inventive method is with short production cycle, low cost, does not produce any poisonous and harmful substance, and products obtained therefrom safety is high, functional activity absorption good, balanced in nutrition, easy to digest, having significant growth promotion, raising infant immunity of organisms, the effect such as replenish the calcium, market application has a extensive future.
Description
Technical field
The present invention relates to a kind of rich in amino acid whose infant marine protein health product and preparation method thereof, belong to health care
Food processing field.
Background technology
Protein is the main matter constituting human tissue organ, plays highly important work in human life activity
With, it is to maintain and repair necessary to body and cell growth, and it not only affects the growth of body tissue, also participates in hormone
All many-sides such as the generation of generation, the maintenance of immunologic function, other nutrient substance and the transhipment of oxygen and hemoglobin, because of
This, protein is to ensure that one of healthy important nutrient.
21 century is numerical ocean model, is the century of Sustainable Development of Marine Economy.In the ocean taking up an area the ball gross area 71%
Live about 400,000 kinds of biologies, take up an area the 80% of ball living species sum.Someone estimates, ocean the most about produces 1350 × 108
Ton organic carbon.In the case of not destroying ecological balance, ocean can provide 30 × 10 every year8Ton aquatic products, enough 30,000,000,000 people's foods
With.Also someone calculates, ocean provides the ability of food to the mankind, is equivalent to land, the whole world the provided food of cultivated area
1000 times.China not only has wide land, and has unbroken coastline, total up to more than 1.8 ten thousand kilometer, is in the world
One of country that coastline is the longest, marine protein aboundresources.At present, the amount of fishing of marine fish, shrimp, shellfish etc. oneself reach 93,000,000
t/a.These protein resources in addition to part is directly the most edible, most of protein resource of low value and the leftover bits and pieces of processing of aquatic products or
By squeezing, drying and be processed into fish flour, or discard, value-added content of product is the lowest.The waste water of fish flour processing is directly discharged into coastal waters
Waters, causes the severe contamination of offshore waters.Therefore, the marine protein to low value carries out high-valued, resource, ecology
Utilize, be that current marine biotechnology is badly in need of the content that conducts a research.Wherein, use fermentation engineering and enzyme engineering technology, be out
Send out the important channel utilizing marine protein resource.Protein is after protease hydrolyzed, and it is low that its skin product has molecular weight, breast
Change heat energy power effective, suitable to increase, be easily absorbed by organisms and the advantage such as new biological activity, make them can be applied to many
Field, especially has broad application prospects in field of health care food.
Infant, at the important stage grown up, needs to absorb a large amount of protein and various nutritional labeling, how to obtain nutrition
Equilibrium, dissolubility egg albumen powder good, absorption easy to digest are the targets that this area is pursued always.Therefore, exploitation infant ocean
Albumen health product can meet infant care demand, again can high-valued exploitation marine protein resource, cultivate novel sea
Economical.But directly dry after marine protein product mostly is protein purification at present and pulverize or prepare through simple enzymolysis process, product
Middle amino acid content is on the low side, not only affects the performance of marine protein functional activity, and have impact on marine protein health promoting product
Palatability.
Summary of the invention
The present invention provides a kind of rich in amino acid whose infant marine protein health product and preparation method thereof, scientific compatibility
Multiple marine protein raw material, utilizes fermentable and multistep cellulase hydrolysis technology to obtain and keeps healthy rich in amino acid whose marine protein
Product, cultivates novel sea economy on the basis of realizing the high-valued exploitation of marine protein, meets infant growth health care
Demand.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The present invention provides a kind of rich in amino acid whose infant marine protein health product and preparation method thereof, it is characterised in that tool
Body comprises the following steps:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
It is (2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%,
Regulation pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of guarantors
Temperature 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: add relative to marine protein mass ratio 3～5% molasses, 1～2% in the marine protein after hydrolysis
Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2～3%, 1～2% inoculation ratio
Example is seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: add relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 albumen
Enzyme, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) it is spray-dried: concentrated solution is carried out spray drying and obtains product infant marine protein health product.
The present invention provides a kind of rich in amino acid whose infant marine protein health product and preparation method thereof, described ocean
Albumen is shark protein, lactalbumin, Antarctic krill egg albumen powder and the mixture of Stichopus japonicus albumen, and its quality group becomes shark protein
20～30 parts, lactalbumin 30～40 parts, Antarctic krill protein 20～30 parts, Stichopus japonicus protein 10～20 parts.
The present invention provides a kind of rich in amino acid whose infant marine protein health product and preparation method thereof, the sea obtained
Ocean albumen health-care product color and luster is brilliant white, powder, free from admixture, without bitterness, connected fermentable effect and makes in product not
Contain only marine protein, and the most certain tropina, total protein content >=98% in product, the most water-soluble after measured
Property protein content >=99%；Gross protein middle-molecular-weihydroxyethyl peptide content >=90% below 1000 dalton；Amino acid content >=
72%, amino acid content equalizes, and the particularly comparision contents of essential amino acids is high, and its ratio is apparently higher than WHO/FAO standard
(35.38%).The reducing power of marine protein health product reaches 0.507, and ABTS radical scavenging activity reaches 1.96mmol/L
Trolox.The nutrition digestibility more existing report level utilizing zoopery to measure marine protein health product exceeds 38.44%；Tool
Having significant growth promotion, raising infant immunity of organisms, the effect such as replenish the calcium, market application has a extensive future；And experiment knot
Fruit shows to change marine protein raw material composition or production technology can significantly reduce marine protein health product effect (P < 0.01).
Detailed description of the invention
Embodiment 1:
A kind of rich in amino acid whose infant marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: accurately join by shark protein 30 parts, lactalbumin 30 parts, 30 parts of Antarctic krill albumen, Stichopus japonicus protein 10 part
Material；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 2%, 1%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulations
3h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 1h；
(7) preparation culture medium: add relative to marine protein mass ratio 5% molasses, 2% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: Candida utilis, saccharomyces cerevisiae are inoculated by relative marine protein mass ratio 3%, 2% inoculative proportion
To culture medium, cultivate 12h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 2%SM98011 protease, 3%
Flavor protease, enzymolysis 2h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product infant marine protein health product.
Embodiment 2:
A kind of rich in amino acid whose infant marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: accurately join by shark protein 20 parts, lactalbumin 40 parts, Antarctic krill protein 20 part, Stichopus japonicus protein 20 part
Material；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1%, 0.5%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 60min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulations
4h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 2h；
(7) preparation culture medium: add relative to marine protein mass ratio 3% molasses, 1% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: Candida utilis, saccharomyces cerevisiae are inoculated by relative marine protein mass ratio 2%, 1% inoculative proportion
To culture medium, cultivate 24h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 1%SM98011 protease, 2%
Flavor protease, enzymolysis 3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product infant marine protein health product.
Embodiment 3:
A kind of rich in amino acid whose infant marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: accurately join by shark protein 25 parts, lactalbumin 35 parts, Antarctic krill protein 25 part, Stichopus japonicus protein 15 part
Material；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1.5%, 0.8%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 45min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2.5 ‰, regulates pH6.0～7.0,60 DEG C of insulations
3.5h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 4 ‰, controls 40 DEG C of hydrolysis 1.5h；
(7) preparation culture medium: add relative to marine protein mass ratio 4% molasses, 1.5% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.35% ammonium sulfate, 0.15% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2.5%, 1.5% inoculative proportion
It is seeded to culture medium, cultivates 18h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 1.5%SM98011 protease,
2.5% flavor protease, enzymolysis 2.5h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product infant marine protein health product.
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to aforementioned reality
Execute example to be described in detail by invention, but for the person of ordinary skill of the art, still can be to aforementioned reality
Execute the technical scheme described in example to modify, or wherein portion of techniques feature is carried out equivalent；And these are revised
Or replace, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (2)
1. the preparation method rich in amino acid whose infant marine protein health product, it is characterised in that specifically include following
Step:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
It is (2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%,
Regulation pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of guarantors
Temperature 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: add relative to marine protein mass ratio 3～5% molasses, 1～2% in the marine protein after hydrolysis
Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2～3%, 1～2% inoculation ratio
Example is seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: add relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 albumen
Enzyme, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) it is spray-dried: concentrated solution is carried out spray drying and obtains product infant marine protein health product.
A kind of preparation method rich in amino acid whose infant marine protein health product the most according to claim 1, its
It is characterised by that described marine protein is shark protein, lactalbumin, Antarctic krill egg albumen powder and the mixture of Stichopus japonicus albumen, its matter
Amount consists of shark protein 20～30 parts, lactalbumin 30～40 parts, Antarctic krill protein 20～30 parts, Stichopus japonicus protein 10～20
Part.
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| CN104263794A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process technique for preparing wheat gluten powder peptide by fermentation and enzymolysis method |
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| CN105624250A (en) * | 2016-03-09 | 2016-06-01 | 广东海洋大学 | Enzymolysis-fermentation coupled aquatic protein active peptide preparation method |
| CN107603799A (en) * | 2017-10-31 | 2018-01-19 | 无锡甜丰食品有限公司 | A kind of method for producing rich nitrogen specialized syrup of beer |
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| CN104187682A (en) * | 2014-09-25 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Preparation technology for blood-sugar-reducing health Chinese herbal gluten peptides |
| CN104263794A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process technique for preparing wheat gluten powder peptide by fermentation and enzymolysis method |
| CN105624249A (en) * | 2016-03-09 | 2016-06-01 | 广东海洋大学 | Preparation method of aquatic protein and plant protein compound active peptide |
| CN105624250A (en) * | 2016-03-09 | 2016-06-01 | 广东海洋大学 | Enzymolysis-fermentation coupled aquatic protein active peptide preparation method |
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Application publication date: 20161109 |