CN105996021A - Anti-aging marine protein healthcare product rich in amino acid and preparing method thereof - Google Patents
Anti-aging marine protein healthcare product rich in amino acid and preparing method thereof Download PDFInfo
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- 238000000034 method Methods 0.000 title abstract description 6
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- 238000000855 fermentation Methods 0.000 claims abstract description 12
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- 239000000047 product Substances 0.000 claims description 37
- 230000036541 health Effects 0.000 claims description 23
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- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses an anti-aging marine protein healthcare product rich in amino acid and a preparing method thereof. Multiple marine raw materials are in scientific compatibility, the anti-aging marine protein healthcare product rich in amino acid is obtained through microbial fermentation and a multistep continuous enzymolysis technology, novel marine economy is cultivated on the basis of marine protein high-valued development and utilization, the anti-aging healthcare requirement is met, and the living standard is improved. The marine healthcare product obtained through ultrasonic-assisted enzymolysis, aerobic fermentation process and secondary enzymolysis is crystal white in color, powdery and free of impurities and bitter taste, the total protein content is larger than or equal to 98%, and the content of peptide with the molecular weight smaller than 1000 daltons in the total protein is larger than or equal to 90%. The method is short in production cycle and low in cost and does not generate toxic and harmful substances, and the obtained product is high in safety, good in functional activity, balanced in nutrition, easy to absorb and digest and remarkable in anti-ageing effect.
Description
Technical field
The present invention relates to a kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, belong to health food manufacture field.
Background technology
Protein is the main matter constituting human tissue organ, human life activity plays a very important role, it is to maintain and repair necessary to body and cell growth, it not only affects the growth of body tissue, all many-sides such as the generation also participating in the generation of hormone, the maintenance of immunologic function, other nutrient substance and the transhipment of oxygen and hemoglobin, therefore, protein is to ensure that one of healthy important nutrient.
21 century is numerical ocean model, is the century of Sustainable Development of Marine Economy.Live about 400,000 kinds of biologies in the ocean taking up an area the ball gross area 71%, take up an area the 80% of ball living species sum.Someone estimates, ocean the most about produces 1350 × 108Ton organic carbon.In the case of not destroying ecological balance, ocean can provide 30 × 10 every year8Ton aquatic products, enough 30,000,000,000 people eat.Also someone calculates, ocean provides the ability of food to the mankind, is equivalent to 1000 times of land, the whole world the provided food of cultivated area.China not only has wide land, and has unbroken coastline, total up to more than 1.8 ten thousand kilometer, is one of coastline is the longest in the world country, marine protein aboundresources.At present, the amount of fishing of marine fish, shrimp, shellfish etc. oneself reach 93,000,000 t/a.These protein resources are in addition to part is directly the most edible, and the major part protein resource of low value and the leftover bits and pieces of processing of aquatic products or be processed into fish flour by squeezing, drying, or discarded, value-added content of product is the lowest.The waste water of fish flour processing is directly discharged into offshore waters, causes the severe contamination of offshore waters.Therefore, the marine protein of low value is carried out high-valued, resource, eco-utilization, be that current marine biotechnology is badly in need of the content that conducts a research.Wherein, use fermentation engineering and enzyme engineering technology, be the important channel developing marine protein resource.Protein is after protease hydrolyzed, it is low that its skin product has molecular weight, emulsifying effectiveness is good, suitable heat energy power increases, be easily absorbed by organisms and the advantage such as new biological activity, makes them can be applied to many fields, especially have broad application prospects in field of health care food.
Aging is the progressive process that each organ of body engenders that along with being continuously increased of age hypofunction and reserve capabillity decline, with advancing age, the same miopragia of various organs of human body, produces various pathological changes, little by little enters ageing state.Therefore prevention and slow down aging have become current social medical research focus.Therefore, exploitation defying age marine protein health product can meet anti-ageing healthcare demand, again can high-valued exploitation marine protein resource, cultivate novel sea economy.But directly drying after marine protein product mostly is protein purification at present and pulverize or prepare through simple enzymolysis process, in product, amino acid content is on the low side, not only affects the performance of marine protein functional activity, and have impact on the palatability of marine protein health promoting product.
Summary of the invention
The present invention provides a kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, scientific compatibility multiple marine protein raw material, fermentable and multistep cellulase hydrolysis technology is utilized to obtain rich in amino acid whose marine protein health promoting product, novel sea economy is cultivated on the basis of realizing the high-valued exploitation of marine protein, meet anti-ageing healthcare demand, advance in living standard.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The present invention provides a kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, it is characterised in that specifically include following steps:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
(2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%, regulate pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasonic frequency is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of insulations 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: adding in the marine protein after hydrolysis relative to marine protein mass ratio 3～5% molasses, 1～2% Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by relative marine protein mass ratio 2～3%, 1～2% inoculative proportion, Candida utilis, saccharomyces cerevisiae are seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: adding relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 protease, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) it is spray-dried: concentrated solution is carried out spray drying and obtains product defying age marine protein health product.
The present invention provides a kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, described marine protein is deep-sea cod collagen protein, Penaeus vannamei albumen, Oyster Protein powder and the mixture of abalone proteins, and its quality group becomes deep-sea cod collagen protein 20～30 parts, Penaeus vannamei albumen 30～40 parts, Oyster Protein 20～30 parts, abalone proteins 10～20 parts.
The present invention provides a kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, the marine protein health-care product color and luster obtained is brilliant white, powder, free from admixture, without bitterness, connected fermentable effect and made product does not contain only marine protein, and the most certain tropina, total protein content >=98% in product, wherein water-soluble protein content >=99% after measured；Gross protein middle-molecular-weihydroxyethyl peptide content >=90% below 1000 dalton；Amino acid content >=70%, amino acid content equalizes, and the particularly comparision contents of essential amino acids is high, and its ratio is apparently higher than WHO/FAO standard (35.38%).The reducing power of marine protein health product reaches 0.502, and ABTS radical scavenging activity reaches 1.94mmol/L
Trolox.Zoopery is utilized to measure the oxidation resistance of marine protein health product, result show mice respectively organize in the content relatively matched group of MDA averagely reduce by 27.11%, relatively aging model group averagely declines 37.15%, during mice is respectively organized, the relatively matched group of SOD vigor averagely improves 39.29%, and relatively aging model group averagely improves 42.55%；And test result indicate that change marine protein raw material composition or production technology can significantly reduce marine protein health product effect (P < 0.01).At present, this product has carried out human feeding trial, and anti-aging effects is notable, and market application has a extensive future.
Detailed description of the invention
Embodiment 1:
A kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: by deep-sea cod collagen protein 30 parts, 30 parts of Penaeus vannamei albumen, Oyster Protein 30 parts, 10 parts of accurate feed proportionings of abalone proteins；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 2%, 1%, regulate pH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasonic frequency is 400w, 25kHz, enzymolysis 30min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulation 3h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 1h；
(7) preparation culture medium: adding relative to marine protein mass ratio 5% molasses, 2% Semen Maydis pulp, 0.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate in the marine protein after hydrolysis, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: by relative marine protein mass ratio 3%, 2% inoculative proportion, Candida utilis, saccharomyces cerevisiae are seeded to culture medium, cultivates 12h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: adding relative marine protein raw materials quality in fermentation liquid than 2%SM98011 protease, 3% flavor protease, enzymolysis 2h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product defying age marine protein health product.
Embodiment 2:
A kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: by deep-sea cod collagen protein 20 parts, 40 parts of Penaeus vannamei albumen, Oyster Protein 20 parts, 20 parts of accurate feed proportionings of abalone proteins；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1%, 0.5%, regulate pH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasonic frequency is 400w, 25kHz, enzymolysis 60min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulation 4h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 2h；
(7) preparation culture medium: adding relative to marine protein mass ratio 3% molasses, 1% Semen Maydis pulp, 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate in the marine protein after hydrolysis, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: by relative marine protein mass ratio 2%, 1% inoculative proportion, Candida utilis, saccharomyces cerevisiae are seeded to culture medium, cultivates 24h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: adding relative marine protein raw materials quality in fermentation liquid than 1%SM98011 protease, 2% flavor protease, enzymolysis 3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product defying age marine protein health product.
Embodiment 3:
A kind of rich in amino acid whose defying age marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: by deep-sea cod collagen protein 25 parts, 35 parts of Penaeus vannamei albumen, Oyster Protein 25 parts, 15 parts of accurate feed proportionings of abalone proteins；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1.5%, 0.8%, regulate pH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasonic frequency is 400w, 25kHz, enzymolysis 45min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2.5 ‰, regulates pH6.0～7.0,60 DEG C of insulation 3.5h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 4 ‰, controls 40 DEG C of hydrolysis 1.5h；
(7) preparation culture medium: adding relative to marine protein mass ratio 4% molasses, 1.5% Semen Maydis pulp, 0.35% ammonium sulfate, 0.15% potassium dihydrogen phosphate in the marine protein after hydrolysis, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: by relative marine protein mass ratio 2.5%, 1.5% inoculative proportion, Candida utilis, saccharomyces cerevisiae are seeded to culture medium, cultivates 18h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: adding relative marine protein raw materials quality in fermentation liquid than 1.5%SM98011 protease, 2.5% flavor protease, enzymolysis 2.5h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product defying age marine protein health product.
Above example is merely to illustrate technical scheme, rather than is limited；Although being described in detail by invention with reference to previous embodiment, but for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent；And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (2)
1. the preparation method rich in amino acid whose defying age marine protein health product, it is characterised in that specifically include following steps:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
(2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%, regulate pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasonic frequency is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of insulations 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: adding in the marine protein after hydrolysis relative to marine protein mass ratio 3～5% molasses, 1～2% Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by relative marine protein mass ratio 2～3%, 1～2% inoculative proportion, Candida utilis, saccharomyces cerevisiae are seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: adding relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 protease, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) it is spray-dried: concentrated solution is carried out spray drying and obtains product defying age marine protein health product.
A kind of preparation method rich in amino acid whose defying age marine protein health product the most according to claim 1, it is characterized in that described marine protein is deep-sea cod collagen protein, Penaeus vannamei albumen, Oyster Protein powder and the mixture of abalone proteins, its quality group becomes deep-sea cod collagen protein 20～30 parts, Penaeus vannamei albumen 30～40 parts, Oyster Protein 20～30 parts, abalone proteins 10～20 parts.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610485970.4A CN105996021A (en) | 2016-06-29 | 2016-06-29 | Anti-aging marine protein healthcare product rich in amino acid and preparing method thereof |
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| CN109295146A (en) * | 2018-11-07 | 2019-02-01 | 北京康宝利华生物科技有限公司 | Enzyme process abalone enzymatic extract and preparation method thereof |
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