CN105648012A - Method for preparing aquatic protein bioactive peptides by means of solid-state fermentation and liquid-state enzymatic hydrolysis - Google Patents
Method for preparing aquatic protein bioactive peptides by means of solid-state fermentation and liquid-state enzymatic hydrolysis Download PDFInfo
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- CN105648012A CN105648012A CN201610129707.1A CN201610129707A CN105648012A CN 105648012 A CN105648012 A CN 105648012A CN 201610129707 A CN201610129707 A CN 201610129707A CN 105648012 A CN105648012 A CN 105648012A
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Abstract
The invention discloses a method for preparing aquatic protein bioactive peptides by means of solid-state fermentation and liquid-state enzymatic hydrolysis. The method includes main steps of mincing aquatic proteins for standby application, smashing vegetable proteins and then sieving the vegetable proteins by the aid of 60-mesh sieves for standby application; mixing the minced aquatic proteins and the smashed vegetable feed proteins with one another, adding nutrients and water into the minced aquatic proteins and the smashed vegetable feed proteins and uniformly mixing the nutrients, the water, the minced aquatic proteins and the smashed vegetable feed proteins with one another to obtain fermentation media; inoculating Bacillus licheniformis HL-3 capable of producing high-temperature-resistant protein proteases onto the fermentation media; carrying out solid-state aerobic fermentation on the media with inoculated strains at the temperatures of 55-60 DEG C for 38-48 h to obtain solid-state fermentation products; adding the solid-state fermentation products into water and carrying out enzymatic hydrolysis on the solid-state fermentation products at the temperatures of 55 DEC C for 2 h to obtain liquid-state enzymatic hydrolysis products; carrying out filtration, ultra-filtration and nano-filtration on the liquid-state enzymatic hydrolysis products to obtain concentrated solution and freezing and drying the concentrated solution to obtain bioactive peptide finished products. The method has the advantages that technical action conditions are mild, the bioactivity of prepared functional peptides and other functional ingredients of raw materials can be kept to a great extent, working procedures for preparing the aquatic protein bioactive peptides can be simplified, the production cost of the aquatic protein bioactive peptides can be reduced, and the method has an excellent application prospect.
Description
Technical field
The present invention relates to a kind of solid state fermentation and combine the method for preparing aquatic product protein active peptide of liquid enzymolysis, belong to aquatic productsProduct are processed and are utilized field.
Background technology
The little peptide of product after protein digestion not only easily digests, absorbs, but also has anti-hypertension, anti-courage admittedlyAlcohol, antithrombotic forms, improves lipid-metabolism, strengthen human body physical agility, help to set up, promote calcium phosphorus and other trace elementAbsorb, promote brain development, improve memory, strengthen macrophage and B cell viability, strengthen immunologic function, protection epidermis is thinBorn of the same parents, prevent melanin deposition, eliminate the effect such as interior free yl.
About the preparation of aquatic product protein active peptide, domesticly carry out a large amount of research. At present, the system of aquatic product protein active peptidePreparation Method mainly contains these four kinds of the methods that self-dissolving enzymolysis, enzymatic isolation method, liquid fermentation method and solid state fermentation combine with liquid enzymolysis.Patent application 200810195646.4 " endogenous enzymes is prepared the technique of bioactive micro peptide " discloses one and has utilized marine low-value fishThe method of the endogenous enzymes self-dissolving enzymolysis in body is prepared the method for aquatic product protein bioactive micro peptide. Patent applicationA kind of 201410145196.3 " methods of preparing scallop body active peptide " disclose a kind of enzymatic isolation method and have prepared aquatic product protein activityThe method of peptide. Patent application CN102028091A " a kind of method of preparing low molecular weight fish peptide through Bacillus natto fermentation method " discloses oneSolution fermentation is prepared the method for fish protein peptides, and patent application 201210539941.3 is " a kind of by fish protein peptides and and Soybean PeptideThe production method of protein peptides of composition " disclose and a kind ofly combine and prepare aquatic product protein active peptide with liquid enzymolysis by solid state fermentationMethod.
In existing aquatic product protein active peptide technology of preparing, there is following defect. First, existing aquatic product protein active peptideIn technology of preparing, there is the process of high-temperature process. In above technology of preparing, there is high temperature this operation of enzyme of going out; Microorganism fermentationMethod raw material needs sterilizing. These high-temperature process above, can to a certain degree weaken Functional Polypeptides or the aquatic product protein itself of preparationBA in contained other functional components; High-temperature process in addition, can strengthen Mei Lade, makes enzymolysis liquid colorDeepen, for finished product process has increased burden. Secondly, the method for external enzyme enzymolysis needs additional protease, has increased cost;Self-dissolving enzymolysis, due to the deficiency of endogenous enzymes, causes enzymolysis not thorough. In addition, although microbe fermentation method need not additional protease,But raw material needs sterilizing to cause endogenous enzymes inactivation, can not effectively utilize fully endogenous enzymes.
Summary of the invention
The problem existing for existing aquatic product protein active peptide technology of preparing. Main purpose of the present invention is to provide oneThe method of preparing aquatic product protein active peptide of low temperature, to retain better Functional Polypeptides and the contained merit of aquatic product protein itself of preparationThe BA of energy property composition. Another object of the present invention is to provide a kind of method that fermentation method is prepared active peptide, makes micro-Biofermentation and endogenous enzymes enzymolysis process can organically combine. It is to simplify the preparation work of active peptide that the present invention also has an objectOrder, reduces its production cost.
In order to address the above problem, the technical solution adopted in the present invention is as follows: a kind of solid state fermentation is combined liquid enzymolysisThe method of preparing aquatic product protein active peptide, comprise the steps:
(1) by the bacillus licheniformis that can produce thermostable proteinase (Bacilluslicheniformis) HL-of slant preservation3 are linked in LB fluid nutrient medium cooling after sterilizing, cultivate 12h in 55 DEG C, 150r/min, are prepared into bacillus licheniformis(Bacilluslicheniformis) HL-3 seed liquor is for subsequent use;
(2), by for subsequent use after fresh aquatic product protein rubbing, after being pulverized, vegetable protein crosses 60 mesh sieves for subsequent use;
(3) vegetable protein after rubbing aquatic product protein step (2) being made and pulverizing is the ratio of 0.5 ~ 1.5:1 in mass ratioMix, and Ensure Liquid thing and water mixes, obtain final moisture content and be controlled at the fermentation medium in 40%～80% scope;
(4) on the step fermentation medium that so (3) make, access the prepared bacillus licheniformis (Bacillus of step (1)Licheniformis) HL-3 seed liquor, and mix;
(5) solid state fermentation: the fermentation medium that adds bacterium that step (4) is obtained ferments according to following method: fermentation trainingFoster base is divided in the fermentation tray of having sterilized, and charging thickness is 4cm～8cm, and in tray, fermentation medium surface coverage is gone outThe wet gauze of bacterium, then tray is placed in to the shelf top fermentation in sterilized solid fermentation room, the temperature that controls environment is 55 DEG C ~ 60DEG C, fermentation time is 38h～48h;
(6) liquid enzymolysis: step (5) is obtained to the ratio that product by solid-state fermentation is 1:3 with water in mass ratio and mix, and stir allAfter even, join in enzymatic vessel, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h;
(7) processing of finished product: the liquid enzyme hydrolysis products that step (6) is obtained is removed precipitation by filtration and obtained enzymolysis liquid; EnzymolysisThe ultrafiltration that liquid is 5000 daltonian milipore filters by molecular cut off is removed macro-molecular protein and is obtained seeing through liquid; See through liquidThen the nanofiltration that is 200 daltonian NF membrane by molecular cut off is removed amino acid and is obtained concentrate; Concentrate processFreeze drying and obtain active peptide finished product.
Bacillus licheniformis (the Bacillus that can produce thermostable proteinase used in described step (1)Licheniformis) HL-3 is preserved in microorganism fungus kind preservation center, Guangdong Province on January 18th, 2016, and deposit number isGDMCCNo:60003, preservation address is Guangdong Microbes Inst, 5th floors, No. 59 building of compound, No. 100, Xianlie Middle Road, Guangzhou City.
Aquatic product protein in described step (2) is leftovers of tilapia, krill, shrimp head, scallop body, cod leftover bits and piecesMaterial, squid leftover bits and pieces, oyster, blue or green squama fish offal material, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces and stripped tuna leftover bits and piecesIn one. Vegetable protein in described step (2) is a kind of in dregs of beans, peanut meal, Cottonseed Meal and the dish dregs of rice or wherein two kindsAbove mixture.
Nutrients in described step (3) is corn flour, food-grade calcium chloride, food stage frerrous chloride and food grade phosphoric acidThe mixture of hydrogen dipotassium composition. Nutraceutical addition in described step (3) accounts for fermentation training with the nutraceutical quality of addingSupport base gross mass percentage meter, be respectively: corn flour 0.01%～5%, food-grade calcium chloride 0.01%～5%, food stage protochlorideIron 0.01%～5%, food grade phosphoric acid hydrogen dipotassium 0.01%～5%.
In described step (4) inoculum concentration of bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor withThe quality of access seed liquor accounts for fermentation medium gross mass percentage meter, is 1%～15%.
Compared with prior art, the present invention has the following advantages.
(1) whole preparation process does not have high-temperature process, has retained better Functional Polypeptides and the institute of aquatic product protein own of preparationContaining the BA in other functional components. The present patent application adopts high temperature resistant the lichen bacillus ferments, at high temperature barUnder part, can suppress the growth of pathogenic bacteria and spoilage organisms, therefore in this technology culture medium without sterilizing. In addition, this technology adopts superFilter membrane is removed the protease in enzymolysis liquid, without the high temperature enzyme that goes out.
(2) simplify operation, reduced cost. Because the present patent application does not have the go out process of enzyme of high temperature, this not only reducesEnergy consumption, but also can greatly reduce the Maillard reaction of enzymolysis liquid, so the color of enzymolysis liquid is superficial. And follow-up film dividesFrom there being again certain decolorizing effect, therefore in this technology, can, without this program of decolouring, simplify operation, reduce cost.
(3) perfect adaptation of endogenous enzymes enzymolysis and microorganism fermentation. In existing fermentation technique, raw material all needs sterilizing, thisNot only increase energy consumption, and made again the endogenous enzymes inactivation of aquatic product protein. This technology Raw, without sterilizing, has retained aquatic productsThe activity of endogenous enzymes in albumen. And the temperature of microorganism fermentation is consistent with the peak enzymolysis-ability temperature of endogenous enzymes, makes endogenous enzymesEnzymolysis is together with the perfect adaptation of microorganism fermentation energy. Just because of the enzymolysis that makes full use of endogenous enzymes in the present invention, makeObtain the present invention without separately adding protease.
(4) preparation cost not only will be combined liquid zymolysis technique lower than existing solid state fermentation, and ferment effect is also excellentCombine liquid zymolysis technique in existing solid state fermentation. Compared with the technology of preparing active peptide with existing employing fermentation method, thisBright application raw material is without extinction, capable of reducing energy consumption and cost; Because the technical scheme that the present patent application provides is become extinct without high temperature,The enzyme that has retained aquatic product protein endogenous proteinase is lived, and this is that existing employing normal temperature fermentation bacterial classification is prepared active peptide and cannot be accomplished, under the assistance of aquatic product protein endogenous proteinase, the present patent application can additional protease. At high temperature protease enzymeActivity stronger, therefore, this technology adopts high temperature resistant bacterial classification to ferment, the degree of hydrolysis of aquatic product protein will be higher than existing normal temperatureStrain fermentation technology.
The invention provides a kind of solid state fermentation and combine the method for preparing aquatic product protein active peptide of liquid enzymolysis. The present inventionTechnical role mild condition, has retained in the Functional Polypeptides of preparation and aquatic product protein contained other functional components itself moreBA, and simplified preparation section and the production cost that has reduced active peptide, there is good application prospect.
Detailed description of the invention
In order to understand better the present invention, below with regard to kind, the fermentation parameter of the kind of aquatic product protein, vegetable protein with send outThe invention will be further described in conjunction with the embodiments in the selection of ferment mode, but protection scope of the present invention is not limited to this.
Embodiment 1: by the bacillus licheniformis (Bacillus that can produce thermostable proteinase of slant preservationLicheniformis) HL-3 is linked in LB fluid nutrient medium cooling after sterilizing, cultivates 12h, system in 55 DEG C, 150r/minStandby one-tenth bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor is for subsequent use. After fresh krill is rubbedFor subsequent use; After being pulverized, dregs of beans crosses 60 mesh sieves for subsequent use. 60 mesh sieves are pulverized and crossed to the krill of 100 kilograms of rubbings and 100 kilogramsDregs of beans mix, and add 5 kilograms of corn flour, 500 grams of food-grade calcium chlorides, 500 grams of food stage frerrous chlorides, 650 grams of foodLevel dipotassium hydrogen phosphate mixes together with 10 kg of water, obtains the fermentation medium after mixing; Fermented and cultured after mixing adds 10Kilogram bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor, stirs and mixes; PostvaccinalFerment culture medium is divided in the fermentation tray of the alcohol surface sterilization of using 75%, and the thickness of feeding in tray is 8 centimetres, in trayThe wet gauze of fermentation medium surface coverage sterilization treatment, then tray is placed on the shelf in the solid fermentation room of sterilizing and sends outFerment, the temperature that controls environment is 55 DEG C, fermentation time is 45h; After fermentation ends, by product by solid-state fermentation and water be in mass ratioThe ratio of 1:3 is mixed and is stirred, and joins in enzymatic vessel, and controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h; By obtained aboveLiquid enzyme hydrolysis products is removed precipitation by filtration and is obtained enzymolysis liquid, and enzymolysis liquid is 5000 daltonian ultrafiltration by molecular cut offThe ultrafiltration of film is removed macro-molecular protein and is obtained seeing through liquid; Seeing through liquid is then 200 daltonian receiving by molecular cut offThe nanofiltration of filter membrane is removed amino acid and is obtained concentrate; Concentrate obtains active peptide finished product through freeze drying.
Embodiment 2: by the bacillus licheniformis (Bacillus that can produce thermostable proteinase of slant preservationLicheniformis) HL-3 is linked in LB fluid nutrient medium cooling after sterilizing, cultivates 12h, system in 55 DEG C, 150r/minStandby one-tenth bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor is for subsequent use. By the shrimp of fresh Penaeus VannmeiHead is for subsequent use after rubbing; After being pulverized, peanut meal crosses 60 mesh sieves for subsequent use. The shrimp head of 100 kilograms of rubbings and 100 kilograms are pulverized and mistakeThe peanut meal of 60 mesh sieves mixes, and add 6 kilograms of corn flour, 510 grams of food-grade calcium chlorides, 550 grams of food stage frerrous chlorides,580 grams of food grade phosphoric acid hydrogen dipotassiums mix together with 8 kg of water, obtain the fermentation medium after mixing; Fermentation training after mixingSupport and add 9 kilograms of bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor, stir and mix; InoculationAfter fermentation medium be divided in the fermentation tray of the alcohol surface sterilization of using 75%, the thickness of feeding in tray is 4 centimetres,The wet gauze of fermentation medium surface coverage sterilization treatment in tray, then tray is placed in to the shelf in the solid fermentation room of sterilizingTop fermentation, the temperature that controls environment is 60 DEG C, fermentation time is 48h; After fermentation ends, product by solid-state fermentation and water are pressed to qualityThan mixing and stir for the ratio of 1:3, join in enzymatic vessel, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h; To obtain aboveTo liquid enzyme hydrolysis products remove precipitation by filtration and obtain enzymolysis liquid, enzymolysis liquid is 5000 daltonian by molecular cut offThe ultrafiltration of milipore filter is removed macro-molecular protein and is obtained seeing through liquid; Seeing through liquid is then 200 dalton by molecular cut offThe nanofiltration of NF membrane remove amino acid and obtain concentrate; Concentrate obtains active peptide finished product through freeze drying.
Embodiment 3: by the bacillus licheniformis (Bacillus that can produce thermostable proteinase of slant preservationLicheniformis) HL-3 is linked in LB fluid nutrient medium cooling after sterilizing, cultivates 12h, system in 55 DEG C, 150r/minStandby one-tenth bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor is for subsequent use. By the oyster strand after fresh shellingBroken rear for subsequent use; After being pulverized, dregs of beans crosses 60 mesh sieves for subsequent use. 60 mesh sieves are pulverized and crossed to the oyster of 100 kilograms of rubbings and 100 kilogramsDregs of beans mix, and add 4 kilograms of corn flour, 560 grams of food-grade calcium chlorides, 550 grams of food stage frerrous chlorides, 650 grams of foodLevel dipotassium hydrogen phosphate mixes together with 8 kg of water, obtains the fermentation medium after mixing; Fermented and cultured after mixing adds 9 public affairsJin bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor, stirs and mixes; Postvaccinal fermentationCulture medium is divided in the fermentation tray of the alcohol surface sterilization of using 75%, and the thickness of feeding in tray is 6 centimetres, in tray, sends outFerment media surface covers the wet gauze of sterilization treatment, then tray is placed in to the shelf top fermentation in the solid fermentation room of sterilizing,The temperature that controls environment is 57 DEG C, and fermentation time is 46h; After fermentation ends, be 1:3 in mass ratio by product by solid-state fermentation and waterRatio mix and stir, join in enzymatic vessel, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h; By liquid obtained aboveState enzymolysis product is removed precipitation by filtration and is obtained enzymolysis liquid, and enzymolysis liquid is 5000 daltonian milipore filters by molecular cut offUltrafiltration remove macro-molecular protein and obtain see through liquid; Seeing through liquid is then 200 daltonian nanofiltrations by molecular cut offThe nanofiltration of film is removed amino acid and is obtained concentrate; Concentrate obtains active peptide finished product through freeze drying.
Claims (7)
1. the invention discloses a kind of solid state fermentation and combine the method for preparing aquatic product protein active peptide of liquid enzymolysis, its feature existsIn: the method comprises the steps:
(1) by the bacillus licheniformis that can produce thermostable proteinase (Bacilluslicheniformis) HL-3 of slant preservationBe linked in LB fluid nutrient medium cooling after sterilizing, cultivate 12h in 55 DEG C, 150r/min, be prepared into bacillus licheniformis(Bacilluslicheniformis) HL-3 seed liquor is for subsequent use;
(2), by for subsequent use after fresh aquatic product protein rubbing, after being pulverized, vegetable protein crosses 60 mesh sieves for subsequent use;
(3) vegetable protein after rubbing aquatic product protein step (2) being made and pulverizing is the ratio of 0.5 ~ 1.5:1 in mass ratioMix, and Ensure Liquid thing and water mixes, obtain final moisture content and be controlled at the fermentation medium in 40%～80% scope;
(4) on the step fermentation medium that so (3) make, access the prepared bacillus licheniformis (Bacillus of step (1)Licheniformis) HL-3 seed liquor, and mix;
(5) solid state fermentation: the fermentation medium that adds bacterium that step (4) is obtained ferments according to following method: fermentation trainingFoster base is divided in the fermentation tray of having sterilized, and charging thickness is 4cm～8cm, and in tray, fermentation medium surface coverage is gone outThe wet gauze of bacterium, then tray is placed in to the shelf top fermentation in sterilized solid fermentation room, the temperature that controls environment is 55 DEG C ~ 60DEG C, fermentation time is 38h～48h;
(6) liquid enzymolysis: step (5) is obtained to the ratio that product by solid-state fermentation is 1:3 with water in mass ratio and mix, and stir allAfter even, join in enzymatic vessel, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h;
(7) processing of finished product: the liquid enzyme hydrolysis products that step (6) is obtained is removed precipitation by filtration and obtained enzymolysis liquid; EnzymolysisThe ultrafiltration that liquid is 5000 daltonian milipore filters by molecular cut off is removed macro-molecular protein and is obtained seeing through liquid; See through liquidThen the nanofiltration that is 200 daltonian NF membrane by molecular cut off is removed amino acid and is obtained concentrate; Concentrate processFreeze drying and obtain active peptide finished product.
2. a kind of solid state fermentation according to claim 1 is combined the method for preparing aquatic product protein active peptide of liquid enzymolysis,It is characterized in that: bacillus licheniformis (Bacilluslicheniformis) HL-3 used in described step (1) is in GuangdongThe deposit number at province microorganism fungus kind preservation center is GDMCCNo:60003.
3. a kind of solid state fermentation according to claim 1 is combined the method for preparing aquatic product protein active peptide of liquid enzymolysis,It is characterized in that: the aquatic product protein in described step (2) is leftovers of tilapia, krill, shrimp head, scallop body, codUnder leftover bits and pieces, squid leftover bits and pieces, oyster, blue or green squama fish offal material, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces and stripped tunaOne in pin material.
4. a kind of solid state fermentation according to claim 1 is combined the method for preparing aquatic product protein active peptide of liquid enzymolysis,It is characterized in that: the vegetable protein in described step (2) is a kind of in dregs of beans, peanut meal, Cottonseed Meal and the dish dregs of rice or wherein twoMixture more than kind.
5. a kind of solid state fermentation according to claim 1 is combined the method for preparing aquatic product protein active peptide of liquid enzymolysis,It is characterized in that: the nutrients in described step (3) is corn flour, food-grade calcium chloride, food stage frerrous chloride and food stageThe mixture of dipotassium hydrogen phosphate composition.
6. a kind of solid state fermentation according to claim 1 is combined the method for preparing aquatic product protein active peptide of liquid enzymolysis,It is characterized in that: the nutraceutical addition in described step (3) accounts for the total matter of fermentation medium with the nutraceutical quality of addingAmount percentage meter, is respectively: corn flour 0.01%～5%, and food-grade calcium chloride 0.01%～5%, food stage frerrous chloride 0.01%～5%, food grade phosphoric acid hydrogen dipotassium 0.01%～5%.
7. a kind of solid state fermentation according to claim 1 is combined the method for preparing aquatic product protein active peptide of liquid enzymolysis,It is characterized in that: the inoculation of bacillus licheniformis (Bacilluslicheniformis) HL-3 seed liquor in described step (4)Amount accounts for fermentation medium gross mass percentage in the quality of access seed liquor, is 1%～15%.
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Cited By (8)
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| CN105961842A (en) * | 2016-07-13 | 2016-09-28 | 陈雨 | Compound shrimp peptide protein for feed and preparation process thereof |
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| CN106173259A (en) * | 2016-07-13 | 2016-12-07 | 陈雨 | A kind of feedstuff compound fish peptide albumen and preparation technology thereof |
| CN107095198A (en) * | 2017-04-27 | 2017-08-29 | 温州科技职业学院 | The quick method for preparing biological compound calcium powder |
| CN108850686A (en) * | 2018-06-09 | 2018-11-23 | 浙江亿丰海洋生物制品有限公司 | A kind of preparation method of the enzymatic hydrolysis and fermentation squid liver paste for fishes and shrimps opening material |
| CN113755550A (en) * | 2021-08-31 | 2021-12-07 | 海南华研胶原科技股份有限公司 | Marine fish oligopeptide prepared from deep sea cod skin and preparation method thereof |
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| CN114712400A (en) * | 2022-04-13 | 2022-07-08 | 济南瑞隆安生物技术有限公司 | Probiotic fermented clam powder composition capable of reducing blood sugar and blood pressure and preparation method thereof |
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Cited By (9)
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| CN106072665A (en) * | 2016-06-29 | 2016-11-09 | 青岛浩大海洋保健食品有限公司 | A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof |
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| CN106173259A (en) * | 2016-07-13 | 2016-12-07 | 陈雨 | A kind of feedstuff compound fish peptide albumen and preparation technology thereof |
| CN107095198A (en) * | 2017-04-27 | 2017-08-29 | 温州科技职业学院 | The quick method for preparing biological compound calcium powder |
| CN107095198B (en) * | 2017-04-27 | 2020-04-03 | 温州科技职业学院 | Method for rapidly preparing biological compound calcium powder |
| CN108850686A (en) * | 2018-06-09 | 2018-11-23 | 浙江亿丰海洋生物制品有限公司 | A kind of preparation method of the enzymatic hydrolysis and fermentation squid liver paste for fishes and shrimps opening material |
| CN113755550A (en) * | 2021-08-31 | 2021-12-07 | 海南华研胶原科技股份有限公司 | Marine fish oligopeptide prepared from deep sea cod skin and preparation method thereof |
| CN114231584A (en) * | 2021-12-27 | 2022-03-25 | 苏州梅氏健康产业管理有限公司 | Preparation method and application of amino acid polypeptide |
| CN114712400A (en) * | 2022-04-13 | 2022-07-08 | 济南瑞隆安生物技术有限公司 | Probiotic fermented clam powder composition capable of reducing blood sugar and blood pressure and preparation method thereof |
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Application publication date: 20160608 |