CN106035984A - Ocean protein healthcare product rich in amino acid for teenagers and preparation method thereof - Google Patents
Ocean protein healthcare product rich in amino acid for teenagers and preparation method thereof Download PDFInfo
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- CN106035984A CN106035984A CN201610485968.7A CN201610485968A CN106035984A CN 106035984 A CN106035984 A CN 106035984A CN 201610485968 A CN201610485968 A CN 201610485968A CN 106035984 A CN106035984 A CN 106035984A
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 93
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 93
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
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- 230000000694 effects Effects 0.000 claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 4
- 239000000047 product Substances 0.000 claims description 39
- 230000036541 health Effects 0.000 claims description 26
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 20
- 230000007062 hydrolysis Effects 0.000 claims description 16
- 238000006460 hydrolysis reaction Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 15
- 239000004365 Protease Substances 0.000 claims description 14
- 239000012141 concentrate Substances 0.000 claims description 10
- 238000010612 desalination reaction Methods 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000002604 ultrasonography Methods 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
- 235000019419 proteases Nutrition 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 101100421450 Drosophila melanogaster Shark gene Proteins 0.000 claims description 7
- 241000239366 Euphausiacea Species 0.000 claims description 7
- 102000004407 Lactalbumin Human genes 0.000 claims description 7
- 108090000942 Lactalbumin Proteins 0.000 claims description 7
- 241000237509 Patinopecten sp. Species 0.000 claims description 7
- 235000020637 scallop Nutrition 0.000 claims description 7
- 239000004382 Amylase Substances 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 108091005658 Basic proteases Proteins 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 5
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 5
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- 235000019634 flavors Nutrition 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 235000013379 molasses Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
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- 238000011081 inoculation Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 11
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- 230000012010 growth Effects 0.000 abstract description 3
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- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
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- 231100000331 toxic Toxicity 0.000 abstract 1
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- 235000013360 fish flour Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- -1 because of This Proteins 0.000 description 1
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- 210000004556 brain Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses an ocean protein healthcare product rich in amino acid for teenagers and a preparation method thereof. Various ocean protein raw materials are scientifically added; microbial fermentation and multi-step continuous enzymolysis processes are utilized to acquire the ocean protein healthcare product rich in amino acid; novel ocean economy is fostered on the basis of realizing the high-value developing utilization of ocean protein; the healthcare requirement of teenagers is met; and the living standard is promoted. The ocean healthcare product acquired by adopting the ultrasonic-assisted enzymolysis, aerobic fermentation technique and secondary enzymolysis is white in color and luster, is in powder form and is free from impurity and bitter taste; the total protein content is more than or equal to 98%; and the content of peptide with the molecular weight below 1000 Dalton in the total protein is more than or equal to 90%. According to the method provided by the invention, the production period is short, the cost is low, no toxic and harmful matter is generated, and the acquired product has the advantages of high safety, good functional activity, balanced nutrition and easy digestive absorption and has effects of promoting growth and memory and increasing the learning efficiency.
Description
Technical field
The present invention relates to a kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, belong to health care
Food processing field.
Background technology
Protein is the main matter constituting human tissue organ, plays highly important work in human life activity
With, it is to maintain and repair necessary to body and cell growth, and it not only affects the growth of body tissue, also participates in hormone
All many-sides such as the generation of generation, the maintenance of immunologic function, other nutrient substance and the transhipment of oxygen and hemoglobin, because of
This, protein is to ensure that one of healthy important nutrient.
21 century is numerical ocean model, is the century of Sustainable Development of Marine Economy.In the ocean taking up an area the ball gross area 71%
Live about 400,000 kinds of biologies, take up an area the 80% of ball living species sum.Someone estimates, ocean the most about produces 1350 × 108
Ton organic carbon.In the case of not destroying ecological balance, ocean can provide 30 × 10 every year8Ton aquatic products, enough 30,000,000,000 people's foods
With.Also someone calculates, ocean provides the ability of food to the mankind, is equivalent to land, the whole world the provided food of cultivated area
1000 times.China not only has wide land, and has unbroken coastline, total up to more than 1.8 ten thousand kilometer, is in the world
One of country that coastline is the longest, marine protein aboundresources.At present, the amount of fishing of marine fish, shrimp, shellfish etc. oneself reach 93,000,000
t/a.These protein resources in addition to part is directly the most edible, most of protein resource of low value and the leftover bits and pieces of processing of aquatic products or
By squeezing, drying and be processed into fish flour, or discard, value-added content of product is the lowest.The waste water of fish flour processing is directly discharged into coastal waters
Waters, causes the severe contamination of offshore waters.Therefore, the marine protein to low value carries out high-valued, resource, ecology
Utilize, be that current marine biotechnology is badly in need of the content that conducts a research.Wherein, use fermentation engineering and enzyme engineering technology, be out
Send out the important channel utilizing marine protein resource.Protein is after protease hydrolyzed, and it is low that its skin product has molecular weight, breast
Change heat energy power effective, suitable to increase, be easily absorbed by organisms and the advantage such as new biological activity, make them can be applied to many
Field, especially has broad application prospects in field of health care food.
Teenager work and study task is heavy, breeds to health and brings huge adverse effect, to nutraceutical product,
The potential demand of health product of particularly making a noise is bigger.Therefore, exploitation teenager marine protein health product can meet teenager guarantor
Strong demand, again can high-valued exploitation marine protein resource, cultivate novel sea economy.But marine protein product mostly is at present
Directly drying after protein purification and pulverize or prepare through simple enzymolysis process, in product, amino acid content is on the low side, not only affects sea
The performance of ocean protein function activity, and have impact on the palatability of marine protein health promoting product.
Summary of the invention
The present invention provides a kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, scientific compatibility
Multiple marine protein raw material, utilizes fermentable and multistep cellulase hydrolysis technology to obtain and keeps healthy rich in amino acid whose marine protein
Product, cultivates novel sea economy on the basis of realizing the high-valued exploitation of marine protein, meets teenager health care demand,
Advance in living standard.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The present invention provides a kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, it is characterised in that tool
Body comprises the following steps:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
It is (2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%,
Regulation pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of guarantors
Temperature 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: add relative to marine protein mass ratio 3～5% molasses, 1～2% in the marine protein after hydrolysis
Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2～3%, 1～2% inoculation ratio
Example is seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: add relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 albumen
Enzyme, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) it is spray-dried: concentrated solution is carried out spray drying and obtains product teenager marine protein health product.
The present invention provides a kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, described ocean
Albumen is shark protein, scallop albumen, Antarctic krill egg albumen powder and the mixture of lactalbumin, and its quality group becomes shark protein
20～30 parts, scallop albumen 30～40 parts, Antarctic krill protein 20～30 parts, lactalbumin 10～20 parts.
The present invention provides a kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, the sea obtained
Ocean albumen health-care product color and luster is brilliant white, powder, free from admixture, without bitterness, connected fermentable effect and makes in product not
Contain only marine protein, and the most certain tropina, total protein content >=98% in product, the most water-soluble after measured
Property protein content >=99%；Gross protein middle-molecular-weihydroxyethyl peptide content >=90% below 1000 dalton；Amino acid content >=
70%, amino acid content equalizes, and the particularly comparision contents of essential amino acids is high, and its ratio is apparently higher than WHO/FAO standard
(35.38%).The reducing power of marine protein health product reaches 0.502, and ABTS radical scavenging activity reaches 1.94mmol/L
Trolox.Utilize zoopery to measure the oxidation resistance of marine protein health product, result show mice respectively organize in MDA
Content relatively matched group averagely reduces by 26.43%, and relatively aging model group averagely declines 37.02%, mice respectively organize in SOD vigor relatively
Matched group averagely improves 39.06%, and relatively aging model group averagely improves 41.34%；And test result indicate that change marine protein is former
Material composition or production technology can significantly reduce marine protein health product effect (P < 0.01).At present, this product has carried out human body
Test-meal is tested, and growth promotion, brain supplementing action effect are notable, have and preferably promote effects such as remembering, improve learning efficiency, and market should
Wide with promotion prospect.
Detailed description of the invention
Embodiment 1:
A kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: accurately join by shark protein 30 parts, 30 parts of scallop albumen, 30 parts of Antarctic krill albumen, lactalbumin 10 parts
Material；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 2%, 1%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulations
3h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 1h；
(7) preparation culture medium: add relative to marine protein mass ratio 5% molasses, 2% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: Candida utilis, saccharomyces cerevisiae are inoculated by relative marine protein mass ratio 3%, 2% inoculative proportion
To culture medium, cultivate 12h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 2%SM98011 protease, 3%
Flavor protease, enzymolysis 2h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product teenager marine protein health product.
Embodiment 2:
A kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: accurately join by shark protein 20 parts, 40 parts of scallop albumen, Antarctic krill protein 20 part, lactalbumin 20 parts
Material；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1%, 0.5%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 60min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 3 ‰, regulates pH6.0～7.0,60 DEG C of insulations
4h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 5 ‰, controls 40 DEG C of hydrolysis 2h；
(7) preparation culture medium: add relative to marine protein mass ratio 3% molasses, 1% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: Candida utilis, saccharomyces cerevisiae are inoculated by relative marine protein mass ratio 2%, 1% inoculative proportion
To culture medium, cultivate 24h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 1%SM98011 protease, 2%
Flavor protease, enzymolysis 3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product teenager marine protein health product.
Embodiment 3:
A kind of rich in amino acid whose teenager marine protein health product and preparation method thereof, concrete preparation process is as follows:
(1) dispensing: accurately join by shark protein 25 parts, 35 parts of scallop albumen, Antarctic krill protein 25 part, lactalbumin 15 parts
Material；
(2) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
(3) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1.5%, 0.8%, regulation
PH7.0～8.0；
(4) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 45min；
(5) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2.5 ‰, regulates pH6.0～7.0,60 DEG C of insulations
3.5h；
(6) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 4 ‰, controls 40 DEG C of hydrolysis 1.5h；
(7) preparation culture medium: add relative to marine protein mass ratio 4% molasses, 1.5% Semen Maydis in the marine protein after hydrolysis
Slurry, 0.35% ammonium sulfate, 0.15% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(8) sterilizing: 121 DEG C of sterilizing 30min；
(9) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(10) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2.5%, 1.5% inoculative proportion
It is seeded to culture medium, cultivates 18h for 28～32 DEG C；
(11) twice ultrasonic ripple enzymolysis: add in fermentation liquid relative marine protein raw materials quality than 1.5%SM98011 protease,
2.5% flavor protease, enzymolysis 2.5h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(12) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(13) desalination: use membrane separation technique to carry out desalination；
(14) concentrate: vacuum low-pressure concentrates；
(15) it is spray-dried: concentrated solution is carried out spray drying and obtains product teenager marine protein health product.
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to aforementioned reality
Execute example to be described in detail by invention, but for the person of ordinary skill of the art, still can be to aforementioned reality
Execute the technical scheme described in example to modify, or wherein portion of techniques feature is carried out equivalent；And these are revised
Or replace, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (2)
1. the preparation method rich in amino acid whose teenager marine protein health product, it is characterised in that specifically include following
Step:
(1) pretreatment: add water in marine protein in the marine protein raw materials quality ratio than 3～5 times, stir；
It is (2) enzyme-added: to be separately added into papain, alkaline protease in the ratio of marine protein mass ratio 1～2%, 0.5～1%,
Regulation pH7.0～8.0；
(3) ultrasound-assisted enzymolysis: under the conditions of controlled enzymatic hydrolysis temperature 40～50 DEG C, under ultrasonic assistant effect, ultrasound wave frequency
Rate is 400w, 25kHz, enzymolysis 30～60min；
(4) liquefaction: be separately added into amylase in the ratio of marine protein mass ratio 2～3 ‰, regulates pH6.0～7.0,60 DEG C of guarantors
Temperature 3～4h；
(5) saccharifying: be separately added into saccharifying enzyme in the ratio of marine protein mass ratio 3～5 ‰, controls 40 DEG C of hydrolysis 1～2h；
(6) preparation culture medium: add relative to marine protein mass ratio 3～5% molasses, 1～2% in the marine protein after hydrolysis
Semen Maydis pulp, 0.2～0.5% ammonium sulfate, 0.1～0.2% potassium dihydrogen phosphate, adjusting initial pH is 7.0；
(7) sterilizing: 121 DEG C of sterilizing 30min；
(8) Candida utilis, saccharomyces cerevisiae activation: Candida utilis, saccharomyces cerevisiae are coupled with in YPD culture medium,
30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed；Then with 1:100 ratio, seed liquor is received in YPD culture medium,
To saccharomyces cerevisiae and Candida utilis liquid seeds；
(9) aerobic fermentation: by Candida utilis, saccharomyces cerevisiae by relative marine protein mass ratio 2～3%, 1～2% inoculation ratio
Example is seeded to culture medium, cultivates 12～24h for 28～32 DEG C；
(10) twice ultrasonic ripple enzymolysis: add relative marine protein raw materials quality in fermentation liquid than 1～2%SM98011 albumen
Enzyme, 2～3% flavor protease, enzymolysis 2～3h under ultrasonic assistant effect, hydrolysis temperature controls at 40～50 DEG C；
(11) centrifugal: fermentation liquid is centrifuged to obtain fermented supernatant fluid；
(12) desalination: use membrane separation technique to carry out desalination；
(13) concentrate: vacuum low-pressure concentrates；
(14) it is spray-dried: concentrated solution is carried out spray drying and obtains product teenager marine protein health product.
A kind of preparation method rich in amino acid whose teenager marine protein health product the most according to claim 1, its
It is characterised by that described marine protein is shark protein, scallop albumen, Antarctic krill egg albumen powder and the mixture of lactalbumin, its matter
Amount consists of shark protein 20～30 parts, scallop albumen 30～40 parts, Antarctic krill protein 20～30 parts, lactalbumin 10～20
Part.
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| CN113508896A (en) * | 2021-05-27 | 2021-10-19 | 南宁山万生物科技有限公司 | Method for preparing seafood seasoning base material by utilizing cane molasses enzymatic method and application |
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| CN104187682A (en) * | 2014-09-25 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Preparation technology for blood-sugar-reducing health Chinese herbal gluten peptides |
| CN104263794A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process technique for preparing wheat gluten powder peptide by fermentation and enzymolysis method |
| CN105624249A (en) * | 2016-03-09 | 2016-06-01 | 广东海洋大学 | Preparation method of aquatic protein and plant protein compound active peptide |
| CN105624250A (en) * | 2016-03-09 | 2016-06-01 | 广东海洋大学 | Enzymolysis-fermentation coupled aquatic protein active peptide preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104187682A (en) * | 2014-09-25 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Preparation technology for blood-sugar-reducing health Chinese herbal gluten peptides |
| CN104263794A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process technique for preparing wheat gluten powder peptide by fermentation and enzymolysis method |
| CN105624249A (en) * | 2016-03-09 | 2016-06-01 | 广东海洋大学 | Preparation method of aquatic protein and plant protein compound active peptide |
| CN105624250A (en) * | 2016-03-09 | 2016-06-01 | 广东海洋大学 | Enzymolysis-fermentation coupled aquatic protein active peptide preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113508896A (en) * | 2021-05-27 | 2021-10-19 | 南宁山万生物科技有限公司 | Method for preparing seafood seasoning base material by utilizing cane molasses enzymatic method and application |
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