CN101717281B - Method for preparing edible fungi growth promoter by protein-containing waste - Google Patents
Method for preparing edible fungi growth promoter by protein-containing waste Download PDFInfo
- Publication number
- CN101717281B CN101717281B CN200910273154A CN200910273154A CN101717281B CN 101717281 B CN101717281 B CN 101717281B CN 200910273154 A CN200910273154 A CN 200910273154A CN 200910273154 A CN200910273154 A CN 200910273154A CN 101717281 B CN101717281 B CN 101717281B
- Authority
- CN
- China
- Prior art keywords
- protein
- edible fungi
- containing waste
- days
- waste
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002699 waste material Substances 0.000 title claims abstract description 27
- 241000233866 Fungi Species 0.000 title claims abstract description 23
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 14
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 12
- 239000007952 growth promoter Substances 0.000 title claims abstract description 9
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims abstract description 9
- 235000011941 Tilia x europaea Nutrition 0.000 claims abstract description 9
- 239000004571 lime Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 150000001413 amino acids Chemical class 0.000 claims description 9
- 230000007062 hydrolysis Effects 0.000 claims description 9
- 238000006460 hydrolysis reaction Methods 0.000 claims description 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 3
- 239000012460 protein solution Substances 0.000 claims description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 33
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract description 3
- 239000000413 hydrolysate Substances 0.000 abstract 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 235000001603 Pleurotus ostreatus Nutrition 0.000 abstract 1
- 240000001462 Pleurotus ostreatus Species 0.000 abstract 1
- 235000012343 cottonseed oil Nutrition 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 238000004904 shortening Methods 0.000 abstract 1
- 235000013305 food Nutrition 0.000 description 11
- 235000001014 amino acid Nutrition 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 238000004064 recycling Methods 0.000 description 4
- 239000002893 slag Substances 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000003531 protein hydrolysate Substances 0.000 description 3
- 235000015099 wheat brans Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000255789 Bombyx mori Species 0.000 description 2
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- 244000234623 Coprinus comatus Species 0.000 description 2
- 235000004439 Coprinus comatus Nutrition 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 210000003746 feather Anatomy 0.000 description 2
- 210000000003 hoof Anatomy 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 244000045069 Agrocybe aegerita Species 0.000 description 1
- 235000008121 Agrocybe aegerita Nutrition 0.000 description 1
- 244000028550 Auricularia auricula Species 0.000 description 1
- 235000000023 Auricularia auricula Nutrition 0.000 description 1
- 240000005710 Auricularia polytricha Species 0.000 description 1
- 235000000024 Auricularia polytricha Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000060234 Gmelina philippensis Species 0.000 description 1
- 240000001080 Grifola frondosa Species 0.000 description 1
- 235000007710 Grifola frondosa Nutrition 0.000 description 1
- 241000577951 Hydnum Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241000315067 Hymenopellis radicata Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000158441 Pleurotus sajor caju Species 0.000 description 1
- 235000004116 Pleurotus sajor caju Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- 235000012255 calcium oxide Nutrition 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method for preparing edible fungi growth promoter by protein-containing waste, comprising: adding 5-6% of lime (relative dry matter) in to the protein-containing waste; hydrolyzing for 1-2h at the high temperature of 121-155 DEG C in a sealed reaction kettle; filtering to remove residue or not remove residue; and adding protein-containing hydrolysate into a culture medium used for planting edible fungi, thus increasing the yield of the edible fungi, shortening the growth cycle and prolonging the fruiting time; therefore, the protein-containing hydrolysate can be used for culturing multiple edible fungis. 2-15% of protein-containing hydrolysate is added into the culture medium containing cottonseed hull and water, oyster mushroom seeds can be generated, and mushrooms come out after about seven days. The fruiting time is more than 3-5 days earlier than the common condition and is prolonged for 7-10 days, and the yield is increased by more than 50%. The method provides rich nitrogen source for the edible fungi, has the advantages of short growth cycle, high production yield and good mouthfeel, realizes reclamation of the protein-containing waste and reduces the secondary pollution of the protein-containing waste.
Description
Technical field
What the present invention relates to is the method for edible fungi growth promoter, and particularly a kind of utilization contains the method that the albumen waste prepares edible fungi growth promoter.
Technical background
Edible mushrooms be a kind of can be the valuable source of food and pharmaceutical industry for human consumption, macroscopic meat, colloid or corky macro fungi.China from the 1950's to the calendar year 2001 between, obtained the success of domesticating and cultivating hedgehog hydnum and white fungus.Realized the extensive cultivation of auricularia auriculajudae, mushroom, white fungus, flat mushroom, Pleurotus sajor-caju, sliding mushroom, needle mushroom, Auricularia polytricha (Mout) Sacc., Twospore Mushroom, Grifola frondosa, Coprinus comatus, Agrocybe aegerita (Brig) Sing, Oudemansiella Radicata; Make the ultimate production of China edible mushrooms become world Edible Fungi big country; Account for 65% of Gross World Product, 22,700,000,000 yuan of the edible mushrooms gross output values.Also set up edible mushrooms research institution in many developed countries; Like mushroom research centre, U.S. University of Pennsylvania; Britain bohr Wen Si institute, Japanese bacterium wingceltis institute, Holland mushroom research centre; Fungal studies institute of Korea S Academy of Agricultural Sciences etc., and obtaining lot of research aspect the researchs such as edible mushrooms classification, ecology, physiology, heredity, breeding, cultivation, disease and pest.
Usually edible mushrooms is all growing after inoculation on its corresponding substratum, and these substratum are used compositions such as cotton seed hulls, wheat bran, cow dung, chicken manure, wood chip and other auxiliary material more.Also to apply growth stimulant in the edible fungus culturing.These growth stimulants are with the produced in conventional processes of hydrochloric acid, sodium hydroxide hydrolysis albumen waste in the prior art, and their potential of hydrogen is high, to the output of edible mushrooms, and growth cycle, mouthfeel is all influential.
Present technique is according to the artificial cultivation technique principle of edible mushrooms (like flat mushroom, mushroom, bisporous mushroom, Coprinus comatus, pork tripe mushroom etc.), and raw materials used basic characteristics (like cotton seed hulls, wheat bran, cow dung, chicken manure, wood chip and other auxiliary material).Proposition contains the albumen waste through lime hydrolysis becoming peptide class and amino acid whose mixed solution (often being called protein liquid); As the growth stimulant technical thought in the edible fungus culturing; To improve the output of edible mushrooms, shorten growth cycle, and improve mouthfeel; Simultaneously, also realize containing the recycling of albumen waste.
Summary of the invention
The present invention proposes and a kind ofly contain the albumen waste, and filter and produce peptide class, edible fungi growth promoter that aminoacids content is high with unslaked lime hydrolysis food factories.
The present invention is achieved in that its production stage is:
1, collects brewer's grains, sugar refinery's rice slag, slaughterhouse hair, hoof tips, blood, food and drink residue food, country fair fish scale, pluck, feather, food factory and milk company WWT mud, silkworm chrysalis, vinasse etc. and contain the albumen waste;
2, add lime 5%-6% (dry matter weight relatively) with containing the albumen waste, add 0.5-2.0 times of water, in the sealed reactor hydrolysis 1-2 hour, temperature was 121-152 ℃;
3, filter the protein solution mixed solution that obtains containing protein peptide class and amino acid composition;
4, be edible fungi growth promoter behind the interpolation 1-5 water doubly.
The present invention can be directly and Edible Fungi raw materials mix fermentation (traditional way of Edible Fungi) back inoculation culture edible mushrooms; Or other preparation of raw material are directly added the present invention after finishing again; But direct inoculation is cultivated after the mixing, is sprayed on the fruit body of edible fungi surface after also can passing through dilution.Because being protein peptide class and amino acid mixing liquid, this hydrolyzate forms.Mikrobe can directly transform or directly absorb, and has realized that the edible mushrooms microorganism growth is fast, growth is sturdy, and output is high, mouthfeel is good; Simultaneously also realized containing the recycling of albumen waste.
Mechanism of the present invention is such; The waste of food factories, food and beverage enterprise, country fair and relevant sludge of sewage treatment plant contain a large amount of protein, and heavy metal free ion and harmful organic and inorganic thing etc.; Through the lime pyrohydrolysis can be peptide class, amino acid mixing liquid with contained proteolysis; Peptide class, amino acid can directly be utilized by mikrobe, remove mikrobe self from and produce proteolytic enzyme, again the protein raw materials enzymolysis are become the biological process of utilizing again behind peptide class or the amino acid; For edible mushrooms provides abundant nitrogenous source, also realized containing the recycling of albumen waste simultaneously.
The present invention has unique advantage
1, the characteristics that food factories contains the albumen waste have been made full use of; As brewer's grains, sugar refinery's rice slag, slaughterhouse hair, hoof tips, blood, food and drink residue food, country fair fish scale, pluck, feather, food factory and milk company WWT mud, silkworm chrysalis, vinasse, etc. contain the albumen waste; Replace utilizing in traditional planting edible mushroom process wheat bran, Semen Maydis powder, the lime raw material of price higher (2500 yuan/ton); Realized that also this type of waste carries out recycling, alleviated and pollute the second time that contains the albumen waste.
2, utilize the lime hydrolysis to contain the albumen waste; Filter the peptide class that arrives, aminoacid mixture filtrating; This filtrating can be directly be sprayed on the sporophore surface with other raw materials mix preparation growth mediums of plantation edible mushrooms or edible fungi growth to the certain period; To improve edible mushrooms output and to improve mouthfeel, shorten growth cycle, the salt concn that method caused that has also overcome traditional hydrochloric acid, sodium hydroxide hydrolysis albumen waste high and influence growing of edible mushrooms shortcoming.
3, can realize improving edible mushrooms output more than 50%, the fruiting time shifts to an earlier date 5-7 days, has more the mushroom time lengthening 7-10 days, and mouthfeel is good.
Embodiment
Below in conjunction with embodiment the present invention is further specified.
Embodiment 1 adds lime 5%-6% (dry-matter relatively) with containing the albumen waste; In the sealed reactor hydrolysis 1-2 hour, filter and remove residue or do not remove slag added in the substratum that contains cotton seed hulls, water containing proteolysate 2-15%; Sterilized 20-60 minute for 80-120 ℃; Meet flat mushroom kind 2-5%, 18-25 ℃ of cultivation got final product fruiting about 7 days.Fruiting time ratio general condition descends morning more than 3-5 days, fruiting time lengthening 7-10 days, and ultimate production has more 45-50%.
Embodiment 2 adds lime 5%-6% (dry-matter relatively) with containing the albumen waste, and in the sealed reactor hydrolysis 1-2 hour, filter and remove residue or do not remove slag; Add in the substratum that contains straw, cow dung, water hybrid composting fermentation 3-5 days to containing proteolysate 2-15%, cooling inoculation bisporous mushroom bacterial classification is cultivated fruiting; Or will contain proteolysate to spray on the substratum of compost fermentation, inoculate the voluminous 40-50% of institute's bisporous mushroom output of producing; Growth is fast, and mouthfeel is good.
Claims (1)
1. a utilization contains the method that the albumen waste prepares edible fungi growth promoter, it is characterized in that step is:
1), collection contains the albumen waste;
2), with containing the lime that the albumen waste adds relative dry matter weight 5%-6%, add 0.2--2.0 times of water, in the sealed reactor hydrolysis 1-2 hour, temperature was 121--155 ℃;
3), filter the protein solution mixed solution that obtains containing protein peptide class and amino acid composition;
4), be edible fungi growth promoter behind the interpolation 1-5 water doubly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910273154A CN101717281B (en) | 2009-12-11 | 2009-12-11 | Method for preparing edible fungi growth promoter by protein-containing waste |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910273154A CN101717281B (en) | 2009-12-11 | 2009-12-11 | Method for preparing edible fungi growth promoter by protein-containing waste |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101717281A CN101717281A (en) | 2010-06-02 |
| CN101717281B true CN101717281B (en) | 2012-10-03 |
Family
ID=42431927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910273154A Expired - Fee Related CN101717281B (en) | 2009-12-11 | 2009-12-11 | Method for preparing edible fungi growth promoter by protein-containing waste |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101717281B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580998B (en) * | 2012-02-22 | 2015-05-20 | 湖北大学 | Method for preparing solid repair agent by using protein peptide and repairing soil by using soil repair agent |
| CN105884441A (en) * | 2014-10-09 | 2016-08-24 | 遵义市鼎新菌业有限公司 | Mushrooms culture medium |
| CN106967664A (en) * | 2017-05-04 | 2017-07-21 | 廊坊恒益生物技术有限公司 | A kind of preparation method for the nutriment for promoting microorganism to grow |
| CN112401224B (en) * | 2020-09-08 | 2022-11-15 | 山东省农业科学院农产品研究所 | Edible fungus enzymatic hydrolysate and preparation method thereof |
| CN112089044B (en) * | 2020-09-08 | 2023-09-01 | 沈阳红梅食品有限公司 | Edible fungus seasoning and preparation method thereof |
| CN112251238A (en) * | 2020-10-28 | 2021-01-22 | 武汉市秀谷科技有限公司 | Microbial preparation for farmland heavy metals and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101058522A (en) * | 2007-04-30 | 2007-10-24 | 新疆农业科学院微生物应用研究所 | Leaf surface fertilizer for composite amino acid chelate complex |
-
2009
- 2009-12-11 CN CN200910273154A patent/CN101717281B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101058522A (en) * | 2007-04-30 | 2007-10-24 | 新疆农业科学院微生物应用研究所 | Leaf surface fertilizer for composite amino acid chelate complex |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101717281A (en) | 2010-06-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102603391B (en) | Compound amino acid organic fertilizer and production method of compound amino acid organic fertilizer | |
| CN101717281B (en) | Method for preparing edible fungi growth promoter by protein-containing waste | |
| CN102696458A (en) | Edible fungus compost, production method thereof and edible fungus culture process | |
| CN103102189B (en) | Organic fertilizer formula making use of factory sludge and seafood mushroom dregs and production process of organic fertilizer formula | |
| CN1594232A (en) | Composite amino acid ecological nutritious agent | |
| CN106396807A (en) | Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi | |
| CN103652364A (en) | High-calcium leaf meal feed as well as production method and application thereof | |
| CN107089873B (en) | Cultivation material and cultivation method for cultivating oyster mushrooms by utilizing needle mushroom residues | |
| CN101743844A (en) | Cultivation method of white beech mushroom | |
| CN102550282A (en) | Method for producing edible fungus with high yield by carrying out enzymolysis on crop straws with enzymatic microorganisms | |
| CN107787765A (en) | A kind of method for producing high-quality pleurotus eryngii | |
| CN106495896B (en) | A kind of Pleurotus eryngii waste chaff is culture materials of edible fungi of raw material and preparation method thereof | |
| CN104641942A (en) | Method for cultivating oyster mushroom on mulberry twigs | |
| CN108476863A (en) | A kind of cultivation base stock and preparation method thereof of sliding mushroom | |
| CN105104756B (en) | A kind of mushroom bran protein peptides of alternative fish meal and preparation method thereof and the application in aquaculture | |
| CN101830757A (en) | Vetiver substrate for culturing edible mushrooms | |
| CN106588279A (en) | Cultivation method of selenium-rich edible mushrooms | |
| CN106718021A (en) | A kind of yield Volvaria volvacea cultivation method high | |
| CN105218257A (en) | A kind of pleurotus eryngii cultivating material suppressing miscellaneous bacteria and preparation method thereof | |
| CN1083408C (en) | Rapid fermenting process for preparing multi-element liquid fertilizer of amino acids | |
| CN1114352C (en) | Process for preparing nutritive activated peptide as additive | |
| CN1460403A (en) | Agaricus bisporus dry preparation composite base material and its preparation method | |
| CN102084931B (en) | Method for preparing organic zinc enzyme preparation | |
| CN110447464A (en) | A kind of hickory chick culture substrate and preparation method thereof | |
| AU2021103569A4 (en) | A method cultivating volvariella volvacea with flammulina velutipes mushroom bran |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121003 |