CN105432631A - Use of natural angular furocoumarin compound libanorin in agricultural bactericide - Google Patents
Use of natural angular furocoumarin compound libanorin in agricultural bactericide Download PDFInfo
- Publication number
- CN105432631A CN105432631A CN201510819082.7A CN201510819082A CN105432631A CN 105432631 A CN105432631 A CN 105432631A CN 201510819082 A CN201510819082 A CN 201510819082A CN 105432631 A CN105432631 A CN 105432631A
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- Prior art keywords
- libanorin
- compound
- agricultural
- bactericide
- agricultural chemicals
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- YCXXSERNLQPDHF-HNNXBMFYSA-N Libanorin Chemical compound C1=CC(=O)OC2=C1C=CC1=C2C[C@@H](C(C)(C)OC(=O)C=C(C)C)O1 YCXXSERNLQPDHF-HNNXBMFYSA-N 0.000 title claims abstract description 28
- 150000001875 compounds Chemical class 0.000 title claims abstract description 28
- YCXXSERNLQPDHF-UHFFFAOYSA-N Libanorin Natural products C1=CC(=O)OC2=C1C=CC1=C2CC(C(C)(C)OC(=O)C=C(C)C)O1 YCXXSERNLQPDHF-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 17
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 11
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 title abstract 4
- 201000010099 disease Diseases 0.000 claims abstract description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 13
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 10
- 240000002791 Brassica napus Species 0.000 claims abstract description 8
- 235000006008 Brassica napus var napus Nutrition 0.000 claims abstract description 8
- 239000006002 Pepper Substances 0.000 claims abstract description 8
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 8
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 8
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 8
- 241000221662 Sclerotinia Species 0.000 claims abstract description 8
- 235000021307 Triticum Nutrition 0.000 claims abstract description 8
- 235000016623 Fragaria vesca Nutrition 0.000 claims abstract description 7
- 240000009088 Fragaria x ananassa Species 0.000 claims abstract description 7
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims abstract description 7
- 206010039509 Scab Diseases 0.000 claims abstract description 7
- 240000007594 Oryza sativa Species 0.000 claims abstract description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 6
- 235000009566 rice Nutrition 0.000 claims abstract description 6
- 244000203593 Piper nigrum Species 0.000 claims abstract 3
- 239000002671 adjuvant Substances 0.000 claims description 11
- 239000003905 agrochemical Substances 0.000 claims description 8
- 235000013399 edible fruits Nutrition 0.000 claims description 7
- 238000009472 formulation Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 241000198596 Alternaria tomatophila Species 0.000 claims description 5
- 239000000839 emulsion Substances 0.000 claims description 5
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000002917 insecticide Substances 0.000 claims description 4
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 claims description 3
- 239000005846 Triadimenol Substances 0.000 claims description 3
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 claims description 3
- 235000010081 allicin Nutrition 0.000 claims description 3
- 239000004530 micro-emulsion Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- BAZVSMNPJJMILC-UHFFFAOYSA-N triadimenol Chemical compound C1=NC=NN1C(C(O)C(C)(C)C)OC1=CC=C(Cl)C=C1 BAZVSMNPJJMILC-UHFFFAOYSA-N 0.000 claims description 3
- 238000009736 wetting Methods 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims description 2
- 239000000417 fungicide Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000000375 suspending agent Substances 0.000 claims description 2
- 150000003852 triazoles Chemical class 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 claims 2
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 239000004480 active ingredient Substances 0.000 abstract description 8
- 241000209140 Triticum Species 0.000 abstract description 7
- 208000031888 Mycoses Diseases 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 229930014626 natural product Natural products 0.000 abstract description 2
- 238000001228 spectrum Methods 0.000 abstract description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract 2
- 240000003768 Solanum lycopersicum Species 0.000 abstract 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 231100000419 toxicity Toxicity 0.000 abstract 1
- 238000000034 method Methods 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 241000722363 Piper Species 0.000 description 5
- 150000004040 pyrrolidinones Chemical class 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 241000123650 Botrytis cinerea Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000813090 Rhizoctonia solani Species 0.000 description 3
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 3
- -1 polyoxyethylene Polymers 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920001285 xanthan gum Polymers 0.000 description 3
- 240000008574 Capsicum frutescens Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000125183 Crithmum maritimum Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 description 2
- 241000233616 Phytophthora capsici Species 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical compound C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- RNMDNPCBIKJCQP-UHFFFAOYSA-N 5-nonyl-7-oxabicyclo[4.1.0]hepta-1,3,5-trien-2-ol Chemical compound C(CCCCCCCC)C1=C2C(=C(C=C1)O)O2 RNMDNPCBIKJCQP-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000213004 Alternaria solani Species 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241001105070 Angelica valida Species 0.000 description 1
- 241001290235 Ceratobasidium cereale Species 0.000 description 1
- 241000222201 Colletotrichum capsici Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229940021584 osthol Drugs 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000004562 water dispersible granule Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses use of natural angular furocoumarin compound libanorin in an agricultural bactericide. The bactericide comprising the compound libanorin can be used for preventing agricultural fungal diseases, can effectively prevent crop diseases such as sclerotinia rot of colza, rice sheath blight, strawberry grey mould, wheat scab, pepper anthracnose, and tomato early blight, and particularly has an obvious effect when being used for preventing sclerotinia rot of colza, strawberry grey mould, wheat scab, pepper anthracnose and tomato early blight. The agricultural bactericide taking the natural compound libanorin as an active ingredient has the characteristics of being good in prevention effect, wide in spectrum, low in toxicity, and free of residue, pollution and public hazards, and particularly facilitates environmental protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to the purposes of a kind of natural angle-furocoumarins compound libanorin as active fraction preparation agricultural bacteriocide.
Background technology
The disease of crops is discovered by people in time due to easy unlike insect and weeds, so often cause the ignorance in control and difficulty, thus its harm is just even more serious.It is reported, whole world list is that the plant disease caused by disease fungus just reaches 10,000 kinds, and the loss caused accounts for 10% ~ 30% of crop year total losses.If the plant disease that virus, bacterium and nematode etc. cause also is calculated interior, its loss is just more considerable.
In order to meet people to grain and the growing needs of other living material, have to adopt chemicals with cover crop by the invasion and attack of pest, wherein bactericide plays an important role.There is the natural products of agricultural bactericidal activity, be generally easily absorbed by plants and conduct, little or without poisoning to phyto toxicity, therefore extremely pay attention to.
Compound English name libanorin, molecular formula: C
19h
20o
5, molecular weight 328.3591, CASNo.27303-26-0, derives from samphire Kingsoft Radix Angelicae Sinensis (AngelicavalidaDiels) molecular structure:
Compound libanorin, is dissolved in the organic solvents such as methyl alcohol, ethanol, acetone, ethyl acetate, chloroform.Less to the research of its active function, do not have as agricultural bacteriocide at the pertinent literature agriculturally applied or report so far.
Summary of the invention
The object of the invention is to explore compound libanorin and preparing the application of agricultural bacteriocide as active component.
The present invention is proved by large quantity research, compound libanorin has excellent bactericidal activity as the agricultural bacteriocide that active fraction preparation obtains, can be applicable to the fungal disease on the various crops of control, as: sclerotinia rot of colza, rice sheath blight disease, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato etc.Especially obvious to fungal disease sclerotinia rot of colza, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato control efficiency.
Compound libanorin is that separation and purification obtains from natural plants, also obtains by chemical synthesis process.Compound libanorin is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients.It can also with other agriculture chemical compounding, be mixed and made into insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Auxiliary agent used can adopt calcium dodecyl benzene sulfonate, pyrrolidones, the rare block compound of polyoxyethylene polyoxy third, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other agricultural chemicals used can be organic bactericide class, as triazole bactericidal agent Triadimenol; Botanical fungicide, as allicin etc.
Beneficial effect of the present invention compared with the prior art: compound libanorin is as bactericide, and antimicrobial spectrum is wide, and the lasting period is long, is agriculturally desirable bactericide.Compared with general chemical pesticide, owing to deriving from the extract of natural plants, for economic crops such as various grain, melon and fruit, vegetables, there is low toxicity, noresidue, pollution-free, non-harmful feature, more favorable environment protection.
Embodiment
Below by way of embodiment, effect of the present invention is described further:
Embodiment 1: compound libanorin is to the control of agricultural disease
Strains tested sclerotinite (Sclerotioiaselerotiorum) is separated from sclerotinia rot of colza bacterial strain and obtains; Rhizoctonia solani Kuhn (Thanatephoruscucumeris) is separated from the sick leaf of rice sheath blight disease; Botrytis cinerea (Botrytiscinerea), obtains from grey mould fruit rot of strawberry separation also known as the pathogen of Botrytis cinerea; Fusarium graminearum (Fusarium.graminearm) is separated from wheat scab fringe and obtains; Rhizoctonia cerealis (Rhizoctoniasolani) is separated from wheat sharp eyespot leaf and obtains; Phytophthora capsici (phytophthoracapsici) is separated from capsicum diseased plant; Capsicum thorn dish spore [Colletotrichumcapsici (syd.) Butl.] is separated from pepper anthracnose fruit and obtains; Sporulation (Alternariasolani.) is separated from early blight of tomato leaf and obtains.
Test compound libanorin(is located away from samphire Kingsoft Radix Angelicae Sinensis, is compound libanorin through Spectral Identification, and be dissolved in methyl alcohol and make 1000 μ g/mL mother liquors, 4 DEG C save backup.PDA medium: 200g peeled potatoes is cut into block, after boiling 10min, gets juice, adds 20g agar, is heated to fusing, then adds 20g glucose, be settled to 1000mL with water.
Mycelial growth rate determination method is adopted to measure the suppression of mycelial growth, from PDA flat board, pre-incubated beating for examination bacterium edge gets bacterium dish (diameter 5mm), by reagent agent mother liquor and medium Homogeneous phase mixing, reagent agent ultimate density is made to be 30ppm, mixed liquor impouring diameter is make flat board in the sterilizing culture dish of 9cm, a bacterium dish inoculated by every ware, and mycelia faces up, and often processes repetition 3 times.25 DEG C are cultured to contrast bacterium colony when being covered with culture dish more than 2/3rds, and right-angled intersection method is measured bacterium colony and increased diameter.Growth inhibition ratio is calculated by colony diameter mean value (mm).
With Osthole Osthol for positive reference substance, can find out that from result compound libanorin can prevent and treat sclerotinia rot of colza, rice sheath blight disease, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato etc. effectively, concentration 30ppm is respectively 70.00%, 26.88%, 68.79%, 54.27%, 42.87%, 38.43% to this several disease suppression rate, and specific experiment the results are shown in Table 1; Fully demonstrate the remarkable result of agricultural chemicals of the present invention by experiment, especially obvious to fungal disease sclerotinia rot of colza, rice sheath blight disease and pepper anthracnose control efficiency.
Table 1 compound libanorin(concentration is 30ppm) bacteriostasis rate to agricultural disease
Embodiment 2:
Weigh Compound libanorin and the customary adjuvant such as lignin, diatomite conveniently technique, make the wetting powder that active ingredient concentration is 2%.
Embodiment 3:
Weigh Compound libanorin and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, solvent wet goods customary adjuvant conveniently technique, make the missible oil that active ingredient concentration is 2%.
Embodiment 4:
Weigh Compound libanorin and the customary adjuvant such as wooden sodium sulfonate, kaolin conveniently technique, make the water dispersible granules that active ingredient concentration is 2%.
Embodiment 5:
Weigh Compound libanorin and solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, makes the aqueous emulsion that active ingredient concentration is 1%.
Embodiment 6:
Weigh Compound libanorin and the customary adjuvant such as calcium dodecyl benzene sulfonate, pyrrolidones conveniently technique, make the microemulsion that active ingredient concentration is 1%.
Embodiment 7:
According to ratio Weigh Compound libanorin and the Triadimenol of weight ratio 1:5, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 5%.
Embodiment 8:
According to ratio Weigh Compound libanorin and the allicin of weight ratio 6:1, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 5%.
Claims (5)
1. compound libanorin is preparing the purposes in agricultural bacteriocide as active component.
2. purposes according to claim 1, is characterized in that compound libanorin is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients; Or compound libanorin is mixed with as active component and other agricultural chemicals and insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.
3. purposes according to claim 2, is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule; Other described agricultural chemicals are triazole bactericidal agent Triadimenol or botanical fungicide allicin.
4. purposes according to claim 1, is characterized in that described bactericide is for preventing and treating agricultural fungal evil.
5. purposes according to claim 4, is characterized in that described agricultural fungal evil is sclerotinia rot of colza, rice sheath blight disease, grey mould fruit rot of strawberry, wheat scab, pepper anthracnose, early blight of tomato etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510819082.7A CN105432631B (en) | 2015-11-24 | 2015-11-24 | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510819082.7A CN105432631B (en) | 2015-11-24 | 2015-11-24 | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105432631A true CN105432631A (en) | 2016-03-30 |
| CN105432631B CN105432631B (en) | 2017-12-12 |
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|---|---|---|---|
| CN201510819082.7A Expired - Fee Related CN105432631B (en) | 2015-11-24 | 2015-11-24 | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide |
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| CN (1) | CN105432631B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632799A (en) * | 2020-04-27 | 2021-11-12 | 兰州大学 | Application of praeruptorin A in preventing and treating agricultural diseases |
| CN114656428A (en) * | 2020-12-23 | 2022-06-24 | 浙江清华长三角研究院 | New structural compound for resisting plant pathogenic fungi |
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| CN103141491A (en) * | 2013-03-26 | 2013-06-12 | 江苏省中国科学院植物研究所 | Applications of natural compound as agriculture bactericide |
| CN103858874A (en) * | 2013-04-16 | 2014-06-18 | 江苏省中国科学院植物研究所 | Application of natural compound as agricultural bactericide |
| CN105028435A (en) * | 2015-07-01 | 2015-11-11 | 江苏省中国科学院植物研究所 | Use of natural compound as agricultural fungicide |
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| CN103141491A (en) * | 2013-03-26 | 2013-06-12 | 江苏省中国科学院植物研究所 | Applications of natural compound as agriculture bactericide |
| CN103858874A (en) * | 2013-04-16 | 2014-06-18 | 江苏省中国科学院植物研究所 | Application of natural compound as agricultural bactericide |
| CN105028435A (en) * | 2015-07-01 | 2015-11-11 | 江苏省中国科学院植物研究所 | Use of natural compound as agricultural fungicide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632799A (en) * | 2020-04-27 | 2021-11-12 | 兰州大学 | Application of praeruptorin A in preventing and treating agricultural diseases |
| CN114656428A (en) * | 2020-12-23 | 2022-06-24 | 浙江清华长三角研究院 | New structural compound for resisting plant pathogenic fungi |
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| CN105432631B (en) | 2017-12-12 |
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