CN103141491B - Applications of natural compound as agriculture bactericide - Google Patents
Applications of natural compound as agriculture bactericide Download PDFInfo
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- CN103141491B CN103141491B CN201310098362.4A CN201310098362A CN103141491B CN 103141491 B CN103141491 B CN 103141491B CN 201310098362 A CN201310098362 A CN 201310098362A CN 103141491 B CN103141491 B CN 103141491B
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- prangenone
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Abstract
The invention discloses applications of a natural compound Prangenone as an agriculture bactericide. Serving as a bactericide for preventing and controlling agricultural fungoid and bacterial diseases, the compound Prangenone is capable of effectively preventing and controlling crop diseases such as sclerotinia rot of colza, bacterial blight of rice, scab of wheat, wheat sharp eyespot, and phytophthora capsici, and especially has a significant effect on preventing and controlling the fungoid disease sclerotinia rot of colza and the bacterial disease bacterial blight of rice. The agriculture bactericide made from the natural compound Prangenone as an active ingredient has the characteristics of excellent prevention and control effect, wide spectrum, low toxicity, no residue, no pollution and public nuisance, and is especially conducive to environmental protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to the purposes of a kind of native compound Prangenone as active fraction preparation agricultural bacteriocide.
Background technology
The disease of crops is discovered by people in time due to easy unlike insect and weeds, so often cause the ignorance in control and difficulty, thus its harm is just even more serious.It is reported, whole world list is that the plant disease caused by disease fungus just reaches 10,000 kinds, and the loss caused accounts for 10% ~ 30% of crop year total losses.If the plant disease that virus, bacterium and nematode etc. cause also is calculated interior, its loss is just more considerable.
In order to meet people to grain and the growing needs of other living material, have to adopt chemicals with cover crop by the invasion and attack of pest, wherein bactericide plays an important role.There is the natural products of agricultural bactericidal activity, be generally easily absorbed by plants and conduct, little or without poisoning to phyto toxicity, therefore extremely pay attention to.
Compound Prangenone, molecular formula: C
16h
14o
5.Derive from samphire Qinghai Radix Angelicae Sinensis Angelica nitida Wolff.
Chemical name:
HNMR(CDCl
3)δ：7.76(1H，d，J＝9.6Hz，H-4)，6.37(1H，d，J＝9.6Hz，H-3)，7.66(1H，d，J＝2.2Hz，H-2′)，6.81(1H，d，J＝2.2Hz，H-3′)，7.37(1H，s，H-5)，5.19(2H，s)，3.07(1H，m)，1.22(3H)，1.21(3H)。
Molecular structure:
Compound Prangenone is colorless needle crystals, fusing point 105 ~ 107 DEG C (petroleum ether-ethyl acetate).Be dissolved in the organic solvents such as methyl alcohol, ethanol, acetone, ethyl acetate, chloroform.Less to the research of its active function, do not have as agricultural bacteriocide at the pertinent literature agriculturally applied or report so far.
Summary of the invention
The object of the invention is to explore compound Prangenone and preparing the application of agricultural bacteriocide as active component.
The present invention is proved by large quantity research, compound Prangenone has excellent bactericidal activity as the agricultural bacteriocide that active fraction preparation obtains, can be applicable to the bacterium on the various crops of control and fungal disease, as: sclerotinia rot of colza, bacterial blight of rice, wheat scab, wheat sharp eyespot, Phytophthora capsici are sick.Especially to fungal disease sclerotinia rot of colza and bacterial disease bacterial blight of rice control efficiency remarkable.
Compound Prangenone is that separation and purification obtains from natural plants, also obtains by chemical synthesis process.Compound Prangenone is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients.It can also with other agriculture chemical compounding, be mixed and made into insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Auxiliary agent used can adopt calcium dodecyl benzene sulfonate, pyrrolidones, the rare block compound of polyoxyethylene polyoxy third, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other agricultural chemicals used can be organic bactericide class, as triazole bactericidal agent Triadimenol; Botanical fungicide, as allicin etc.
Beneficial effect of the present invention compared with the prior art: compound Prangenone is as bactericide, and antimicrobial spectrum is wide, and the lasting period is long, is agriculturally desirable bactericide.Compared with general chemical pesticide, owing to deriving from the extract of natural plants, for economic crops such as various grain, melon and fruit, vegetables, there is low toxicity, noresidue, pollution-free, non-harmful feature, more favorable environment protection.
Embodiment
Below by way of embodiment, effect of the present invention is described further:
Embodiment 1: compound Prangenone is to the control of agricultural disease
Strains tested sclerotinite (Sclerotioia selerotiorum) is separated from sclerotinia rot of colza bacterial strain and obtains; Rice Xanthomonas (Xanthomonas oryzae pv.oryzae) is separated from the sick leaf of bacterial blight of rice; Fusarium graminearum (Fusarium.graminearm) is separated from wheat scab fringe and obtains; Rhizoctonia cerealis (Rhizoctonia solani) is separated from wheat sharp eyespot leaf and obtains; Phytophthora capsici (phytophthora capsici) is separated from capsicum diseased plant.
Reagent agent compound Prangenone (being located away from samphire Qinghai Radix Angelicae Sinensis Angelica nitida Wolff, is compound Prangenone through Spectral Identification), be dissolved in acetone and make 1000 μ g/mL mother liquors, 4 DEG C save backup.PDA medium: 200g peeled potatoes is cut into block, after boiling 10min, gets juice, adds 20g agar, is heated to fusing, then adds 20g glucose, be settled to 1000mL with water.
Mycelial growth rate determination method is adopted to measure the suppression of mycelial growth, from PDA flat board, pre-incubated beating for examination bacterium edge gets bacterium dish (diameter 5mm), by reagent agent mother liquor and medium Homogeneous phase mixing, make reagent agent ultimate density be respectively 10,5,2.5,1.25,0ppm, mixed liquor respectively impouring diameter is make flat board in the sterilizing culture dish of 9cm, a bacterium dish inoculated by every ware, and mycelia faces up, and often processes repetition 3 times.25 DEG C are cultured to contrast bacterium colony when being covered with culture dish more than 2/3rds, and right-angled intersection method is measured bacterium colony and increased diameter.Growth inhibition ratio is calculated by colony diameter mean value (mm).To the data DPS V3.01 professional version software analysis process of gained, draw calibration curve and EC
50value.Positive reference substance is Osthole (purchased from Nat'l Pharmaceutical & Biological Products Control Institute), calculates growth inhibition ratio with same concentration same method.
Can find out that from result compound Prangenone can prevent and treat sclerotinia rot of colza, bacterial blight of rice, wheat scab, wheat sharp eyespot, Phytophthora capsici disease etc. effectively, to concentration EC in this several disease suppression
50be 2.92 ~ 83.91ppm, specific experiment the results are shown in Table 1; And positive reference substance Osthole is to concentration EC in sclerotinia rot of colza, bacterial blight of rice, wheat scab, wheat sharp eyespot, Phytophthora capsici these 5 kinds of disease suppressions sick
50be respectively 43.33,16.34,56.94,39.96,34.64ppm, fully demonstrated the remarkable result of agricultural chemicals of the present invention by contrast experiment, especially to fungal disease sclerotinia rot of colza and bacterial disease bacterial blight of rice control efficiency obvious.
Table 1 compound Prangenone is to agricultural disease toxicity test result
Embodiment 2:
Weigh Compound Prangenone and the customary adjuvant such as lignin, diatomite conveniently technique, make the wetting powder that active ingredient concentration is 2%.
Embodiment 3:
Weigh Compound Prangenone and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, solvent wet goods customary adjuvant conveniently technique, make the missible oil that active ingredient concentration is 2%.
Embodiment 4:
Weigh Compound Prangenone and the customary adjuvant such as wooden sodium sulfonate, kaolin conveniently technique, make the water dispersible granules that active ingredient concentration is 2%.
Embodiment 5:
Weigh Compound Prangenone and solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, makes the aqueous emulsion that active ingredient concentration is 1%.
Embodiment 6:
Weigh Compound Prangenone and the customary adjuvant such as calcium dodecyl benzene sulfonate, pyrrolidones conveniently technique, make the microemulsion that active ingredient concentration is 1%.
Embodiment 7:
According to ratio Weigh Compound Prangenone and the Triadimenol of weight ratio 1: 5, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 5%.
Embodiment 8:
According to ratio Weigh Compound Prangenone and the allicin of weight ratio 6: 1, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 5%.
Claims (3)
1. compound pabularinone does harm to the purposes of sclerotinia rot of colza, wheat scab, wheat sharp eyespot, Phytophthora capsici disease and agricultural bacterial evil bacterial blight of rice for preventing and treating agricultural fungal as bacteriostatic active ingredients.
2. purposes according to claim 1, is characterized in that compound pabularinone is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients; Or compound pabularinone is mixed with as active component and other agricultural chemicals and insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.
3. purposes according to claim 2, is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule; Other described agricultural chemicals are triazole bactericidal agent Triadimenol or botanical fungicide allicin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310098362.4A CN103141491B (en) | 2013-03-26 | 2013-03-26 | Applications of natural compound as agriculture bactericide |
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| CN201310098362.4A CN103141491B (en) | 2013-03-26 | 2013-03-26 | Applications of natural compound as agriculture bactericide |
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| CN103141491A CN103141491A (en) | 2013-06-12 |
| CN103141491B true CN103141491B (en) | 2015-05-27 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105432631B (en) * | 2015-11-24 | 2017-12-12 | 江苏省中国科学院植物研究所 | A kind of natural purposes of the angle-furocoumarins compound as agricultural bacteriocide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448057A (en) * | 2002-04-01 | 2003-10-15 | 张水良 | Dressing agent |
| EP2526769A1 (en) * | 2011-05-25 | 2012-11-28 | Cognis IP Management GmbH | Biocide compositions comprising (poly)glycerol glycosides |
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2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448057A (en) * | 2002-04-01 | 2003-10-15 | 张水良 | Dressing agent |
| EP2526769A1 (en) * | 2011-05-25 | 2012-11-28 | Cognis IP Management GmbH | Biocide compositions comprising (poly)glycerol glycosides |
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