CN103141491A - Applications of natural compound as agriculture bactericide - Google Patents
Applications of natural compound as agriculture bactericide Download PDFInfo
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- CN103141491A CN103141491A CN2013100983624A CN201310098362A CN103141491A CN 103141491 A CN103141491 A CN 103141491A CN 2013100983624 A CN2013100983624 A CN 2013100983624A CN 201310098362 A CN201310098362 A CN 201310098362A CN 103141491 A CN103141491 A CN 103141491A
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Abstract
The invention discloses applications of a natural compound Prangenone as an agriculture bactericide. Serving as a bactericide for preventing and controlling agricultural fungoid and bacterial diseases, the compound Prangenone is capable of effectively preventing and controlling crop diseases such as sclerotinia rot of colza, bacterial blight of rice, scab of wheat, wheat sharp eyespot, and phytophthora capsici, and especially has a significant effect on preventing and controlling the fungoid disease sclerotinia rot of colza and the bacterial disease bacterial blight of rice. The agriculture bactericide made from the natural compound Prangenone as an active ingredient has the characteristics of excellent prevention and control effect, wide spectrum, low toxicity, no residue, no pollution and public nuisance, and is especially conducive to environmental protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to a kind of native compound Prangenone as the purposes of active fraction preparation agricultural bacteriocide.
Background technology
The disease of crops is in time discovered by people due to easy unlike insect and weeds, thus often cause ignorance and difficulty in control, thereby its harm is just even more serious.It is reported, the whole world is singly just to reach 10,000 kinds by the plant disease that disease fungus causes, the loss that causes accounts for 10%～30% of crop year total losses.If the plant disease that virus, bacterium and nematode etc. cause is also calculated interior, its loss is just more considerable.
In order to satisfy people to grain and the growing needs of other living material, have to adopt chemicals not to be subjected to the invasion and attack of pest with cover crop, wherein bactericide plays an important role.Natural products with agricultural bactericidal activity, general easy in plant absorbing and conduction, little or without poisoning to phyto toxicity, therefore extremely pay attention to.
Compound Prangenone, molecular formula: C
16H
14O
5Derive from samphire Qinghai Radix Angelicae Sinensis Angelica nitida Wolff.
Chemical name:
HNMR(CDCl
3)δ：7.76(1H，d，J＝9.6Hz，H-4)，6.37(1H，d，J＝9.6Hz，H-3)，7.66(1H，d，J＝2.2Hz，H-2′)，6.81(1H，d，J＝2.2Hz，H-3′)，7.37(1H，s，H-5)，5.19(2H，s)，3.07(1H，m)，1.22(3H)，1.21(3H)。
Molecular structure:
Compound Prangenone is colourless acicular crystal, 105～107 ℃ of fusing points (petroleum ether-ethyl acetate).Be dissolved in the organic solvents such as methyl alcohol, ethanol, acetone, ethyl acetate, chloroform.Less to its active function research, pertinent literature or the report do not used on agricultural as agricultural bacteriocide so far.
Summary of the invention
The objective of the invention is to explore compound Prangenone as the application of active component at the preparation agricultural bacteriocide.
The present invention is by studies have shown that in a large number, the agricultural bacteriocide that compound Prangenone obtains as active fraction preparation has excellent bactericidal activity, can be applicable to bacterium and fungal disease on the various crops of control, as: sclerotinia rot of colza, bacterial blight of rice, wheat scab, wheat sharp eyespot, Phytophthora capsici disease etc.Especially remarkable to fungal disease sclerotinia rot of colza and bacterial disease bacterial blight of rice control efficiency.
Compound Prangenone is that separation and purification obtains from natural plants, also can obtain by chemical synthesis process.Compound Prangenone is mixed with as active component and insecticides adjuvant, excipients any one formulation that allows on agricultural chemicals.It can also with other agriculture chemical compounding, be mixed and made into insecticides adjuvant, excipients any one formulation that allows on agricultural chemicals.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Auxiliary agent used can adopt calcium dodecyl benzene sulfonate, pyrrolidones, the rare block compound of polyoxyethylene polyoxy third, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other agricultural chemicals used can be the organic bactericide class, as the triazole bactericidal agent Triadimenol; Botanical fungicide is as allicin etc.
Beneficial effect of the present invention compared with the prior art: compound Prangenone is as bactericide, and antimicrobial spectrum is wide, and the lasting period is long, is the bactericide of agriculture coideal.Compare with general chemical pesticide, due to the extract that derives from natural plants, have low toxicity, noresidue, pollution-free, non-harmful characteristics for economic crops such as various grains, melon and fruit, vegetables, more favorable environment protection.
Embodiment
Below by embodiment, effect of the present invention is described further:
Embodiment 1: the control of compound Prangenone to agricultural disease
Strains tested sclerotinite (Sclerotioia selerotiorum) obtains from the separation of sclerotinia rot of colza bacterial strain; Rice Xanthomonas (Xanthomonas oryzae pv.oryzae) separates from the sick leaf of bacterial blight of rice; Fusarium graminearum (Fusarium.graminearm) obtains from the separation of wheat scab fringe; Rhizoctonia cerealis (Rhizoctonia solani) obtains from the separation of wheat sharp eyespot leaf; Phytophthora capsici (phytophthora capsici) separates from the capsicum diseased plant.
Reagent agent compound Prangenone (being located away from samphire Qinghai Radix Angelicae Sinensis Angelica nitida Wolff, is compound Prangenone through Spectral Identification) is dissolved in acetone with it and makes 1000 μ g/mL mother liquors, and 4 ℃ save backup.PDA medium: 200g peeling potato is cut into piece, after boiling 10min, gets juice, adds 20g agar, is heated to fusing, then adds 20g glucose, and water is settled to 1000mL.
Adopt the mycelial growth rate determination method that the inhibition of mycelial growth is measured, pre-incubated beating for examination bacterium edge got bacterium dish (diameter 5mm) from the PDA flat board, the reagent agent mother liquor is evenly mixed with medium, make that the reagent agent ultimate density is respectively 10,5,2.5,1.25,0ppm, mixed liquor impouring diameter respectively is to make flat board in the sterilization culture dish of 9cm, every ware inoculation one bacterium dish, mycelia faces up, and every processing repeats 3 times.25 ℃ are cultured to the contrast bacterium colony and are covered with culture dish 2/3rds when above, and the right-angled intersection method is measured bacterium colony growth diameter.Calculate growth inhibition ratio by colony diameter mean value (mm).Data to gained are processed with DPS V3.01 professional version software analysis, draw calibration curve and EC
50Value.Positive reference substance is Osthole (available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute), calculates growth inhibition ratio with same concentration same method.
Can find out that from result compound Prangenone can prevent and treat sclerotinia rot of colza, bacterial blight of rice, wheat scab, wheat sharp eyespot, Phytophthora capsici disease etc. effectively, to concentration EC in these several disease suppressions
50Be 2.92～83.91ppm, concrete experimental result sees Table 1; And the positive reference substance Osthole is to concentration EC in sclerotinia rot of colza, bacterial blight of rice, wheat scab, wheat sharp eyespot, sick these the 5 kinds of disease suppressions of Phytophthora capsici
50Be respectively 43.33,16.34,56.94,39.96,34.64ppm, demonstrated fully the remarkable result of agricultural chemicals of the present invention by the contrast experiment, especially obvious to fungal disease sclerotinia rot of colza and bacterial disease bacterial blight of rice control efficiency.
Table 1 compound Prangenone is to agricultural disease toxicity test result
Embodiment 2:
Take the conventional auxiliary materials such as compound Prangenone and lignin, diatomite according to common process, make active ingredient concentration and be 2% wetting powder.
Embodiment 3:
Take compound Prangenone and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, the conventional auxiliary material of solvent wet goods according to common process, make active ingredient concentration and be 2% missible oil.
Embodiment 4:
Take the conventional auxiliary materials such as compound Prangenone and wooden sodium sulfonate, kaolin according to common process, make active ingredient concentration and be 2% water dispersible granules.
Embodiment 5:
Take compound Prangenone and solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 1% aqueous emulsion.
Embodiment 6:
Take the conventional auxiliary materials such as compound Prangenone and calcium dodecyl benzene sulfonate, pyrrolidones according to common process, make active ingredient concentration and be 1% microemulsion.
Embodiment 7:
Take compound Prangenone and Triadimenol according to the weight ratio ratio of 1: 5, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 5% aqueous emulsion.
Embodiment 8:
Take compound Prangenone and allicin according to the weight ratio ratio of 6: 1, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 5% aqueous emulsion.
Claims (6)
1. compound Prangenone is as the purposes of active component in the preparation agricultural bacteriocide.
2. purposes according to claim 1, is characterized in that compound Prangenone is mixed with as active component and insecticides adjuvant, excipients any one formulation that allows on agricultural chemicals; Perhaps compound Prangenone is mixed with as active component and other agricultural chemicals and insecticides adjuvant, excipients any one formulation that allows on agricultural chemicals.
3. purposes according to claim 2, is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule; Described other agricultural chemicals are triazole bactericidal agent Triadimenol or botanical fungicide allicin.
4. purposes according to claim 1, is characterized in that described bactericide is used for preventing and treating agriculture bacterial disease and fungal disease.
5. purposes according to claim 4, is characterized in that described agriculture bacterial disease is bacterial blight of rice.
6. purposes according to claim 4, is characterized in that described agriculture fungal disease is sclerotinia rot of colza, bacterial blight of rice, wheat scab, wheat sharp eyespot, Phytophthora capsici disease etc.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105432631A (en) * | 2015-11-24 | 2016-03-30 | 江苏省中国科学院植物研究所 | Use of natural angular furocoumarin compound libanorin in agricultural bactericide |
Citations (2)
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|---|---|---|---|---|
| CN1448057A (en) * | 2002-04-01 | 2003-10-15 | 张水良 | Dressing agent |
| EP2526769A1 (en) * | 2011-05-25 | 2012-11-28 | Cognis IP Management GmbH | Biocide compositions comprising (poly)glycerol glycosides |
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2013
- 2013-03-26 CN CN201310098362.4A patent/CN103141491B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448057A (en) * | 2002-04-01 | 2003-10-15 | 张水良 | Dressing agent |
| EP2526769A1 (en) * | 2011-05-25 | 2012-11-28 | Cognis IP Management GmbH | Biocide compositions comprising (poly)glycerol glycosides |
Non-Patent Citations (11)
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| A.CHATTERJEE 等: ""Lactonic constituents of Prangos pabularia Lindl(umbelliferae)"", 《TETRAHEDRON》, vol. 28, no. 20, 31 December 1972 (1972-12-31), pages 5175 - 5182 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105432631A (en) * | 2015-11-24 | 2016-03-30 | 江苏省中国科学院植物研究所 | Use of natural angular furocoumarin compound libanorin in agricultural bactericide |
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