CN103858874A - Application of natural compound as agricultural bactericide - Google Patents
Application of natural compound as agricultural bactericide Download PDFInfo
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- CN103858874A CN103858874A CN201410009288.9A CN201410009288A CN103858874A CN 103858874 A CN103858874 A CN 103858874A CN 201410009288 A CN201410009288 A CN 201410009288A CN 103858874 A CN103858874 A CN 103858874A
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- auraptenol
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Abstract
The invention discloses a natural compound Auraptenol used as a bactericide for preventing and treating agricultural fungal diseases, and the natural compound has a remarkable prevention and treatment effect especially on sclerotinia rot of colza, fusarium wilt of cucumber, a rice sheath blight disease, wheat scab, pepper anthracnose, a rice bakanae disease, wheat sharp eyespot, tomato late blight, gray mold and the like. As an agricultural bactericide, the Auraptenol has the characteristics of good effect, broad spectrum, low toxicity, no residues, no pollution and no harm, and is especially beneficial to environmental protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to the purposes of a kind of native compound Auraptenol as active fraction preparation agricultural bacteriocide.
Background technology
In agricultural production, the pathogen of damage to crops also has fungi, bacterium, virus etc., wherein having the greatest impact of fungi, and crop production reduction is obvious, and the economic loss especially being caused by fungus-caused disease because of plant disease can not be ignored.
Auraptenol, No. CAS: 1221-43-8, chemical name: (+)-8-(2-Hydroxy-3-methyl-3-butenyl)-7-methoxy-2H-1-benzopyran-2-one, molecular structure:
Auraptenol is colourless acicular crystal, is dissolved in the organic solvents such as methyl alcohol, ethanol, acetone and chloroform, little to its activity research, there is no so far its pertinent literature of applying in agricultural as bactericide or report.
Summary of the invention
The object of the invention is to explore Auraptenol and preparing the application of agricultural bacteriocide as active component.
The present invention is by studies have shown that in a large number, the agricultural bacteriocide that Auraptenol obtains as active fraction preparation also has excellent bactericidal activity, can be applicable to the disease on the various crops of control, be particularly suitable for preventing and treating agricultural fungal diseases, as: sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc.
Auraptenol is that separation and purification obtains or synthetic can obtaining from natural plants.Auraptenol is mixed with as active component and insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals.Auraptenol can also and other agriculture chemical compounding, be mixed and made into insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Auxiliary agent used can adopt calcium dodecyl benzene sulfonate, pyrrolidones, the rare block compound of polyoxyethylene polyoxy third, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other agricultural chemicals used are organic phosphates, as: thimet (3911), demeton (1059, E1059), parathion (1605, E605), dichlorvos (DDV), Rogor, chlorophos etc.; Pyrethroid, as: cypermethrin, decamethrin, kill bacterium fat etc.; Microbial source insecticide, as bacillus thuringiensis,Bt (Bt), Avermectin, white muscardine fungi etc.
Beneficial effect of the present invention: Auraptenol is as bactericide compared with the prior art, and antimicrobial spectrum is wide, and the antibacterial time is short, and the lasting period is long, is the bactericide of agriculture coideal.Compared with general chemical pesticide, effect is remarkable, and Auraptenol derives from the extract of natural plants, has low toxicity, noresidue, pollution-free, non-harmful feature for economic crops such as various grain, melon and fruit, vegetables, more favorable environment protection.
Embodiment
By embodiment, effect of the present invention is described further below:
The control of embodiment 1:Auraptenol to agricultural fungal diseases
Strains tested Fusarium graminearum (Fusarium.graminearm) separates and obtains from wheat scab fringe; Fusarium oxysporum (F.oxysporum) separates and obtains from cucumber fusarium axysporum diseased plant; Rhizoctonia solani Kuhn (Rhizoctonia solani) separates and obtains from rice sheath blight disease leaf; Rhizoctonia cerealis (Rhizoctonia solani) separates and obtains from wheat sharp eyespot leaf; Sclerotinite (Sclerotioia selerotiorum) separates and obtains from sclerotinia rot of colza bacterial strain; Fusarium moniliforme (F.moniliform) separates from bakanae disease of rice fringe; Capsicum anthrax-bacilus (Colletotrichum capsici) separates from the sick leaf of capsicum; Botrytis cinerea (Botrytis cinerea) separates from cucumber; Sporulation (Alternariasolani) separates from the sick leaf of early blight of tomato; Phytophthora capsici (phytophthora capsici) separates from capsicum diseased plant.Reagent agent Auraptenol (separating qualification from the samphire root of Dahurain angelica obtains), is dissolved in acetone and is made 1000 μ g/mL mother liquors, and 4 DEG C save backup.PDA medium: 200g peeling potato is cut into piece, boils after 10min, gets juice, adds 20g agar, is heated to fusing, then adds 20g glucose, and water is settled to 1000mL.
Adopt mycelial growth rate determination method to measure the inhibition of hypha,hyphae growth, from PDA flat board, pre-incubated fungi edge is beaten and is got bacterium dish (diameter 5mm), reagent agent mother liquor is evenly mixed with medium, make that reagent agent ultimate density is respectively 0,1,2.5,5,10ppm, in the mixed liquor sterilizing culture dish that impouring diameter is 9cm respectively, make flat board, every ware is inoculated a bacterium dish, and mycelia faces up, and every processing repeats 3 times.25 DEG C are cultured to contrast bacterium colony and are covered with culture dish 2/3rds when above, and right-angled intersection method is measured bacterium colony growth diameter.Calculate growth inhibition ratio by colony diameter mean value (mm).To DPS V3.01 professional version software analysis processing for the data of gained, draw calibration curve and EC
50value.Positive reference substance is Osthole (purchased from Nat'l Pharmaceutical & Biological Products Control Institute), with same concentration same method calculating conk inhibiting rate.
From result, can find out that Auraptenol can prevent and treat sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc. effectively, concentration EC during these several fungal diseases are suppressed
50be 2.84～18.46ppm, specific experiment the results are shown in Table 1; And positive reference substance Osthole is to concentration EC in sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, these 5 kinds of fungal diseases inhibition of wheat sharp eyespot
50be respectively 43.33,41.82,26.84,56.94,39.96ppm, fully demonstrated the remarkable result of agricultural chemicals of the present invention by contrast experiment.
Table 1Auraptenol is to agriculture fungi toxicity test result
Embodiment 2:
Take the conventional auxiliary materials such as Auraptenol and lignin, diatomite according to common process, make active ingredient concentration and be 2% wetting powder.
Embodiment 3:
Take Auraptenol and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, the conventional auxiliary material of solvent wet goods according to common process, make active ingredient concentration and be 2% missible oil.
Embodiment 4:
Claim the conventional auxiliary materials such as Auraptenol and wooden sodium sulfonate, kaolin according to common process, make active ingredient concentration and be 2% water dispersible granules.
Embodiment 5:
Take Auraptenol and solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 1% aqueous emulsion.
Embodiment 6:
Take the conventional auxiliary materials such as Auraptenol and calcium dodecyl benzene sulfonate, pyrrolidones according to common process, make active ingredient concentration and be 1% microemulsion.
Embodiment 7:
Take Auraptenol and thimet according to the weight ratio ratio of 1: 5, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 12% aqueous emulsion.
Embodiment 8:
Take Auraptenol and decamethrin according to the weight ratio ratio of 6: 1, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 21% aqueous emulsion.
Embodiment 9:
Take Auraptenol and Avermectin according to the weight ratio ratio of 1: 3, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 4% aqueous emulsion.
Claims (5)
1.Auraptenol as active component in the purposes of preparing in agricultural bacteriocide.
2. purposes according to claim 1, is characterized in that Auraptenol is mixed with as active component and insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals; Or Auraptenol is mixed with as active component and other agricultural chemicals and insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals.
3. purposes according to claim 2, is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule.
4. purposes according to claim 1, it is characterized in that Auraptenol as bactericide for preventing and treating agricultural fungal diseases.
5. application according to claim 4, is characterized in that described agricultural fungal diseases is sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410009288.9A CN103858874B (en) | 2013-04-16 | 2013-04-16 | Application of natural compound as agricultural bactericide |
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| CN201410009288.9A CN103858874B (en) | 2013-04-16 | 2013-04-16 | Application of natural compound as agricultural bactericide |
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| CN201310129846.0A Division CN103190405B (en) | 2013-04-16 | 2013-04-16 | Use of natural compound as agricultural bactericide or pesticide |
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| CN103858874A true CN103858874A (en) | 2014-06-18 |
| CN103858874B CN103858874B (en) | 2015-04-08 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028047A (en) * | 2015-06-30 | 2015-11-11 | 云南天质网络科技有限公司 | Control method for rice gibberellic disease |
| CN105432631A (en) * | 2015-11-24 | 2016-03-30 | 江苏省中国科学院植物研究所 | Use of natural angular furocoumarin compound libanorin in agricultural bactericide |
| CN106748787A (en) * | 2016-04-23 | 2017-05-31 | 何华琼 | A kind of agricultural bacteriocide and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101456853A (en) * | 2009-01-05 | 2009-06-17 | 江苏省农业科学院 | Bionic novel series compounds 7-alkoxyl-8-(3,3'-disubstituted propyl) benzo pyran-2-ones synthesis and its application as pesticides |
| CN102093322A (en) * | 2010-12-31 | 2011-06-15 | 厦门大学 | Application of osthole in preparation of marine antifouling paint |
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2013
- 2013-04-16 CN CN201410009288.9A patent/CN103858874B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101456853A (en) * | 2009-01-05 | 2009-06-17 | 江苏省农业科学院 | Bionic novel series compounds 7-alkoxyl-8-(3,3'-disubstituted propyl) benzo pyran-2-ones synthesis and its application as pesticides |
| CN102093322A (en) * | 2010-12-31 | 2011-06-15 | 厦门大学 | Application of osthole in preparation of marine antifouling paint |
Non-Patent Citations (2)
| Title |
|---|
| BIKASH RANJAN BARIK: ""coumarins of murraya exotiva -absolute configuration of aurapteno"", 《PHYTOCHEMISTRY》, vol. 22, no. 3, 31 December 1983 (1983-12-31), pages 792 - 794 * |
| 韦献果: ""柑橘及其近缘属植物中天然香豆素化合物及药效作用综述"", 《沈阳药科大学学报》, 15 July 2012 (2012-07-15), pages 343 - 347 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028047A (en) * | 2015-06-30 | 2015-11-11 | 云南天质网络科技有限公司 | Control method for rice gibberellic disease |
| CN105432631A (en) * | 2015-11-24 | 2016-03-30 | 江苏省中国科学院植物研究所 | Use of natural angular furocoumarin compound libanorin in agricultural bactericide |
| CN106748787A (en) * | 2016-04-23 | 2017-05-31 | 何华琼 | A kind of agricultural bacteriocide and preparation method thereof |
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| CN103858874B (en) | 2015-04-08 |
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Address after: 211225 Lishui City, Nanjing province Baima Town National Agricultural Science and Technology Park plant base Applicant after: Institute of Botany, Jiangsu Chinese Academy of Sciences Address before: 210014, No. 1, former village, Xuanwu District, Xuanwu District, Jiangsu, Nanjing Applicant before: Institute of Botany, Jiangsu Chinese Academy of Sciences |
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