CN105028435A

CN105028435A - Use of natural compound as agricultural fungicide

Info

Publication number: CN105028435A
Application number: CN201510376170.4A
Authority: CN
Inventors: 宋萍萍; 韩召军; 王年鹤; 吕晔
Original assignee: Institute of Botany of CAS; Nanjing Agricultural University
Current assignee: Institute of Botany of CAS; Nanjing Agricultural University
Priority date: 2015-07-01
Filing date: 2015-07-01
Publication date: 2015-11-11
Anticipated expiration: 2035-07-01
Also published as: CN105028435B

Abstract

The invention discloses use of a natural compound Pd-c-V(b) as an agricultural fungicide. The compound Pd-c-V(b) serving as the fungicide is used for preventing agricultural fungi diseases, can effectively prevent crop diseases such as rape sclerotinia rot, strawberry grey mould and wheat scab, and particularly has an obvious prevention effect on the fungi diseases namely the rape sclerotinia rot and the wheat scab. The natural compound Pd-c-V(b) serving as the agricultural fungicide prepared from active ingredients has the characteristics of good prevention effect, wide adaptability, low toxicity, no residue, no pollution and no public nuisance, and particularly facilitates environmental protection.

Description

A kind of native compound is as the purposes of agricultural bacteriocide

Technical field

The invention belongs to agricultural technology field, be specifically related to the purposes of a kind of natural line style dihydropyran coumarin compound Pd-c-V (b) as active fraction preparation agricultural bacteriocide.

Background technology

The disease of crops is discovered by people in time due to easy unlike insect and weeds, so often cause the ignorance in control and difficulty, thus its harm is just even more serious.It is reported, whole world list is that the plant disease caused by disease fungus just reaches 10,000 kinds, and the loss caused accounts for 10% ~ 30% of crop year total losses.If the plant disease that virus, bacterium and nematode etc. cause also is calculated interior, its loss is just more considerable.

In order to meet people to grain and the growing needs of other living material, have to adopt chemicals with cover crop by the invasion and attack of pest, wherein bactericide plays an important role.There is the natural products of agricultural bactericidal activity, be generally easily absorbed by plants and conduct, little or without poisoning to phyto toxicity, therefore extremely pay attention to.

Compound Pd-c-V (b), molecular formula: C ₂₁h ₂₂o ₇.Derive from samphire RADIX PEUCEDANI angelicadecursiva.

ESI-MSm／z：387[M+H] ⁺,HNMR(CDC1 ₃)δ：7.60(1H,d，J=9.6Hz，H-4),6.23(1H,d,J=9.6Hz,H-3),6.81(1H,s,H-8),7.39(1H,s,H-5),6.08(1H,d，J=6.0Hz，H-4')，5.25(1H,d，J=6.0Hz,H-3')，6.17(1H),1.84(3H),2.02(3H),2.07(3H)。

Molecular structure:

Pd-c-Ⅴ(b)R=-O-ace,R'=-O-ang

Compound Pd-c-V (b) is colorless needle crystals.Be dissolved in the organic solvents such as methyl alcohol, ethanol, acetone, ethyl acetate, chloroform.Less to the research of its active function, do not have as agricultural bacteriocide at the pertinent literature agriculturally applied or report so far.

Summary of the invention

The object of the invention is to explore compound Pd-c-V (b) and preparing the application of agricultural bacteriocide as active component.

The present invention is proved by large quantity research, compound Pd-c-V (b) has excellent bactericidal activity as the agricultural bacteriocide that active fraction preparation obtains, can be applicable to the fungal disease on the various crops of control, as: sclerotinia rot of colza, grey mould fruit rot of strawberry, wheat scab etc.Especially to fungal disease sclerotinia rot of colza and wheat scab control efficiency obvious.

Compound Pd-c-V (b) is that separation and purification obtains from natural plants, also obtains by chemical synthesis process.Compound Pd-c-V (b) is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients.It can also with other agriculture chemical compounding, be mixed and made into insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Auxiliary agent used can adopt calcium dodecyl benzene sulfonate, pyrrolidones, the rare block compound of polyoxyethylene polyoxy third, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other agricultural chemicals used can be organic bactericide class, as triazole bactericidal agent Triadimenol; Botanical fungicide, as allicin etc.

Beneficial effect of the present invention compared with the prior art: compound Pd-c-V (b) is as bactericide, and antimicrobial spectrum is wide, and the lasting period is long, is agriculturally desirable bactericide.Compared with general chemical pesticide, owing to deriving from the extract of natural plants, for economic crops such as various grain, melon and fruit, vegetables, there is low toxicity, noresidue, pollution-free, non-harmful feature, more favorable environment protection.

Embodiment

Below by way of embodiment, effect of the present invention is described further:

Embodiment 1: compound Pd-c-V (b) is to the control of agricultural disease

Strains tested sclerotinite ( sclerotioiasclerotiorum) obtain from the separation of sclerotinia rot of colza bacterial strain; Fusarium graminearum ( fusarium.graminearum) obtain from the separation of wheat scab fringe; The pathogen of Botrytis cinerea ( botrytiscinerea) obtain from the separation of Botrytis cinerea diseased plant.

Reagent agent compound Pd-c-V (b) (being located away from samphire RADIX PEUCEDANI, is compound Pd-c-V (b) through Spectral Identification), be dissolved in acetone and make 1000 μ g/mL mother liquors, 4 DEG C save backup.PDA medium: 200g peeled potatoes is cut into block, after boiling 10min, gets juice, adds 20g agar, is heated to fusing, then adds 20g glucose, be settled to 1000mL with water.

Mycelial growth rate determination method is adopted to measure the suppression of mycelial growth, from PDA flat board, pre-incubated beating for examination bacterium edge gets bacterium dish (diameter 5mm), by reagent agent mother liquor and medium Homogeneous phase mixing, make reagent agent ultimate density be respectively 30,20,10,5,0ppm, mixed liquor respectively impouring diameter is make flat board in the sterilizing culture dish of 9cm, a bacterium dish inoculated by every ware, and mycelia faces up, and often processes repetition 3 times.25 DEG C are cultured to contrast bacterium colony when being covered with culture dish more than 2/3rds, and right-angled intersection method is measured bacterium colony and increased diameter.Growth inhibition ratio is calculated by colony diameter mean value (mm).To the data DPSV3.01 professional version software analysis process of gained, draw calibration curve and EC ₅₀value.

Can find out that from result compound Pd-c-V (b) can prevent and treat sclerotinia rot of colza, grey mould fruit rot of strawberry, wheat scab etc., effectively to concentration EC in this several disease suppression ₅₀be 13.17 ~ 71.89ppm, specific experiment the results are shown in Table 1; Fully demonstrated the remarkable result of agricultural chemicals of the present invention by experiment, especially to fungal disease sclerotinia rot of colza and wheat scab control efficiency obvious.

table 1 compound Pd-c-V (b) is to agricultural disease toxicity test result

Embodiment 2: Weigh Compound Pd-c-V (b) and the customary adjuvant such as lignin, diatomite conveniently technique, make the wetting powder that active ingredient concentration is 2%.

Embodiment 3:

Weigh Compound Pd-c-V (b) and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, solvent wet goods customary adjuvant conveniently technique, make the missible oil that active ingredient concentration is 2%.

Embodiment 4:

Weigh Compound Pd-c-V (b) and the customary adjuvant such as wooden sodium sulfonate, kaolin conveniently technique, make the water dispersible granules that active ingredient concentration is 2%.

Embodiment 5:

Weigh Compound Pd-c-V (b) and solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, makes the aqueous emulsion that active ingredient concentration is 1%.

Embodiment 6:

Weigh Compound Pd-c-V (b) and the customary adjuvant such as calcium dodecyl benzene sulfonate, pyrrolidones conveniently technique, make the microemulsion that active ingredient concentration is 1%.

Embodiment 7:

According to ratio Weigh Compound Pd-c-V (b) and the Triadimenol of weight ratio 1:5, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 5%.

Embodiment 8:

According to ratio Weigh Compound Pd-c-V (b) and the allicin of weight ratio 6:1, with solvent naphtha, pyrrolidones, the customary adjuvant such as xanthans conveniently technique, make the aqueous emulsion that active ingredient concentration is 5%.

Claims

1. compound Pd-c-V (b) is preparing the purposes in agricultural bacteriocide as active component.

2. purposes according to claim 1, is characterized in that compound Pd-c-V (b) is mixed with any one formulation that agricultural chemicals allows as active component and insecticides adjuvant, excipients; Or compound Pd-c-V (b) is mixed with as active component and other agricultural chemicals and insecticides adjuvant, excipients any one formulation that agricultural chemicals allows.

3. purposes according to claim 2, is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule; Other described agricultural chemicals are triazole bactericidal agent Triadimenol or botanical fungicide allicin.

4. purposes according to claim 1, is characterized in that described bactericide is for preventing and treating agricultural fungal evil.

5. purposes according to claim 4, is characterized in that described agricultural fungal evil is sclerotinia rot of colza, grey mould fruit rot of strawberry, wheat scab etc.