CN103190405B - Use of natural compound as agricultural bactericide or pesticide - Google Patents
Use of natural compound as agricultural bactericide or pesticide Download PDFInfo
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- CN103190405B CN103190405B CN201310129846.0A CN201310129846A CN103190405B CN 103190405 B CN103190405 B CN 103190405B CN 201310129846 A CN201310129846 A CN 201310129846A CN 103190405 B CN103190405 B CN 103190405B
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Abstract
The invention discloses use of a natural compound Auraptenol as an agricultural bactericide or pesticide. The Auraptenol can be taken as the pesticide for preventing and treating Lepidoptera and homopetera insects or the bactericide for preventing and treating agricultural fungus diseases, and especially has remarkable control efficiency for sclerotinia rot of colza, fusarium wilt of cucumber, rice sheath blight disease, scab of wheat, anthracnose of hot pepper, rice bakanae disease, wheat sharp eyespot, tomato late blight, gray mold and the like. The Auraptenol as the agricultural bactericide or pesticide is good in effect, broad in spectrum, low in toxicity, free from residue, non-pollution and nuisanceless, and is especially in favor of environment protection.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to the purposes of a kind of native compound Auraptenol as active fraction preparation agricultural fungicides or bactericide.
Background technology
Pest occur in the many regional large area of China, and frequency is in rising trend, and harm increases the weight of.At present still take chemical pesticide control as major control measure.Due to the long-term chemical pesticide control that uses, cause insect to produce pesticide resistance in various degree to number of chemical insecticides such as organochlorine, organic phosphor, carbamate, pyrethroids, control efficiency significantly declines, make peasant have to strengthen dosage to improve preventive effect, but reduced again the safety of agricultural product.
In addition, in agricultural production, the pathogen of damage to crops also has fungi, bacterium, virus etc., wherein having the greatest impact of fungi, and crop production reduction is obvious, and the economic loss especially being caused by fungus-caused disease because of plant disease can not be ignored.
Auraptenol, No. CAS: 1221-43-8, chemical name: (+)-8-(2-Hydroxy-3-methyl-3-butenyl)-7-methoxy-2H-1-benzopyran-2-one, molecular structure:
Auraptenol is colourless acicular crystal, is dissolved in the organic solvents such as methyl alcohol, ethanol, acetone and chloroform, little to its activity research, the pertinent literature or the report that do not have so far it to apply in agricultural as bactericide or insecticide.
Summary of the invention
The object of the invention is to explore Auraptenol as active component in the application of preparing agricultural fungicides or bactericide.
The present invention is by studies have shown that in a large number, and the agricultural fungicides that Auraptenol obtains as active fraction preparation has good insecticidal activity, can be used for preventing and treating various pest on crop, especially for control Lepidoptera and Homoptera insect.Lepidopterous insects can be Noctuidae, diamond-back moth section, Sulfur butterfly insect; Described noctuid is preferably prodenia litura, beet armyworm, mythimna separata; Diamond-back moth section insect is preferably diamond-back moth; Sulfur butterfly insect is preferably cabbage caterpillar.Described Homoptera insect is Aphidiadae insect, is preferably black peach aphid.
The agricultural bacteriocide that Auraptenol obtains as active fraction preparation also has excellent bactericidal activity, can be applicable to the disease on the various crops of control, be particularly suitable for preventing and treating agricultural fungal diseases, as: sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc.
Auraptenol is that separation and purification obtains or synthetic can obtaining from natural plants.Auraptenol is mixed with as active component and insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals.Auraptenol can also and other agriculture chemical compounding, be mixed and made into insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals.Described formulation can be missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule etc.Auxiliary agent used can adopt calcium dodecyl benzene sulfonate, pyrrolidones, the rare block compound of polyoxyethylene polyoxy third, polyoxyethylene nonylphenol ether etc., and excipient can be cyclohexanone, solvent naphtha, solvent naphtha, water, propane diols, deionized water etc.Composite other agricultural chemicals used are organic phosphates, as: thimet (3911), demeton (1059, E1059), parathion (1605, E605), dichlorvos (DDV), Rogor, chlorophos etc.; Pyrethroid, as: cypermethrin, decamethrin, kill bacterium fat etc.; Microbial source insecticide, as bacillus thuringiensis,Bt (Bt), Avermectin, white muscardine fungi etc.
Beneficial effect of the present invention: Auraptenol is as insecticide or bactericide compared with the prior art, and insecticidal spectrum, antimicrobial spectrum are wide, and desinsection, antibacterial time are short, and the lasting period is long, is insecticide, the bactericide of agriculture coideal.Compared with general chemical pesticide; insect is had tag, stomach poison function, effect is remarkable, and Auraptenol derives from the extract of natural plants; there is low toxicity, noresidue, pollution-free, non-harmful feature for economic crops such as various grain, melon and fruit, vegetables, more favorable environment protection.
Embodiment
By embodiment, effect of the present invention is described further below:
The control of embodiment 1:Auraptenol to agricultural fungal diseases
Strains tested Fusarium graminearum (Fusarium.graminearm) separates and obtains from wheat scab fringe; Fusarium oxysporum (F.oxysporum) separates and obtains from cucumber fusarium axysporum diseased plant; Rhizoctonia solani Kuhn (Rhizoctonia solani) separates and obtains from rice sheath blight disease leaf; Rhizoctonia cerealis (Rhizoctonia solani) separates and obtains from wheat sharp eyespot leaf; Sclerotinite (Sclerotioia selerotiorum) separates and obtains from sclerotinia rot of colza bacterial strain; Fusarium moniliforme (F.moniliform) separates from bakanae disease of rice fringe; Capsicum anthrax-bacilus (Colletotrichum capsici) separates from the sick leaf of capsicum; Botrytis cinerea (Botrytis cinerea) separates from cucumber; Sporulation (Alternariasolani) separates from the sick leaf of early blight of tomato; Phytophthora capsici (phytophthora capsici) separates from capsicum diseased plant.Reagent agent Auraptenol (separating evaluation from the samphire root of Dahurain angelica obtains), is dissolved in acetone and is made 1000 μ g/mL mother liquors, and 4 ℃ save backup.PDA medium: 200g peeling potato is cut into piece, boils after 10min, gets juice, adds 20g agar, is heated to fusing, then adds 20g glucose, and water is settled to 1000mL.
Adopt mycelial growth rate determination method to measure the inhibition of hypha,hyphae growth, from PDA flat board, pre-incubated fungi edge is beaten and is got bacterium dish (diameter 5mm), reagent agent mother liquor is evenly mixed with medium, make that reagent agent ultimate density is respectively 0,1,2.5,5,10ppm, in the mixed liquor sterilizing culture dish that impouring diameter is 9cm respectively, make flat board, every ware is inoculated a bacterium dish, and mycelia faces up, and every processing repeats 3 times.25 ℃ are cultured to contrast bacterium colony and are covered with culture dish 2/3rds when above, and right-angled intersection method is measured bacterium colony growth diameter.Calculate growth inhibition ratio by colony diameter mean value (mm).To DPS V3.01 professional version software analysis processing for the data of gained, draw calibration curve and EC
50value.Positive reference substance is Osthole (purchased from Nat'l Pharmaceutical & Biological Products Control Institute), with same concentration same method calculating conk inhibiting rate.
From result, can find out that Auraptenol can prevent and treat sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, pepper anthracnose, bakanae disease of rice, wheat sharp eyespot, tomato late blight, gray mold etc. effectively, concentration EC during these several fungal diseases are suppressed
50be 2.84～18.46ppm, specific experiment the results are shown in Table 1; And positive reference substance Osthole is to concentration EC in sclerotinia rot of colza, cucumber fusarium axysporum, rice sheath blight disease, wheat scab, these 5 kinds of fungal diseases inhibition of wheat sharp eyespot
50be respectively 43.33,41.82,26.84,56.94,39.96ppm, fully demonstrated the remarkable result of agricultural chemicals of the present invention by contrast experiment.
Table 1Auraptenol is to agriculture fungi toxicity test result
Embodiment 2:Auraptenol as active component to agricultural insect management
Be that field population 3～6 instar larvaes that gather are raised and laid eggs to pupating at indoor artificial feed for examination worm source, select indoor F1 and F2 for the basically identical larva of size for toxicity test (wherein, cabbage caterpillar is field collection population).Accurately take Auraptenol (separating evaluation from the samphire root of Dahurain angelica obtains), be dissolved in acetone, making concentration is the confession examination mother liquor of 2.000mg/ml, is then diluted to 6 different concentration as for subsequent use for reagent liquid by 1 ‰ TritonX-100 aqueous emulsions.With defoliator by fresh clean cabbage leaves (wherein, for mythimna separata, wheat leaf blade, tea geometrid are fresh tea leaf in its) make the leaf dish that diameter is 1.5cm, then it is soaked in for reagent liquid and within 10 seconds, take out afterwards, rear taking-up is dried and is placed in the culture dish that contains 1% water agar or moisturizing filter paper, access the examination worm of hungry 3h, be placed in artificial climate indoor [(25 ± 1) ℃, L/D=14/10, RH75%], the liquid of each concentration is processed 30 cephalonts.Blank group adds 1 ‰ Triton100 aqueous emulsion dipping blades with acetone, and positive reference substance is Osthole (purchased from Nat'l Pharmaceutical & Biological Products Control Institute), processes and tries worm with the same leaf dipping method of same concentration.48h checks lethality, calculates the dead borer population of each processing, the borer population of living, and calculates larval mortality.
Result shows: in 48h, reagent agent is effective to prodenia litura, beet armyworm, diamond-back moth, cabbage caterpillar, mythimna separata, black peach aphid, and concrete lethality, in table 2, has been tested the virulence activity to anomala corpulenta adult in addition, and it is invalid to chafer that result shows; And positive controls, concentration is that the lethality that examination worm prodenia litura, beet armyworm, mythimna separata, the black peach aphid of 1.000mg/ml Osthole processing tags is all less than 40%.Show by comparative trial, Auraptenol, as agricultural insecticide, has the advantage that the desinsection time is short, desinsection scope is wide.
The virulence test result of table 2Auraptenol to insect
Embodiment 3:
Take the conventional auxiliary materials such as Auraptenol and lignin, diatomite according to common process, make active ingredient concentration and be 2% wetting powder.
Embodiment 4:
Take Auraptenol and calcium dodecyl benzene sulfonate, phenethyl phenol polyethenoxy ether, the conventional auxiliary material of solvent wet goods according to common process, make active ingredient concentration and be 2% missible oil.
Embodiment 5:
Claim the conventional auxiliary materials such as Auraptenol and wooden sodium sulfonate, kaolin according to common process, make active ingredient concentration and be 2% water dispersible granules.
Embodiment 6:
Take Auraptenol and solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 1% aqueous emulsion.
Embodiment 7:
Take the conventional auxiliary materials such as Auraptenol and calcium dodecyl benzene sulfonate, pyrrolidones according to common process, make active ingredient concentration and be 1% microemulsion.
Embodiment 8:
Take Auraptenol and thimet according to the weight ratio ratio of 1: 5, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 12% aqueous emulsion.
Embodiment 9:
Take Auraptenol and decamethrin according to the weight ratio ratio of 6: 1, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 21% aqueous emulsion.
Embodiment 10:
Take Auraptenol and Avermectin according to the weight ratio ratio of 1: 3, with solvent naphtha, pyrrolidones, the conventional auxiliary material such as xanthans is according to common process, makes active ingredient concentration and be 4% aqueous emulsion.
Claims (8)
1.Auraptenol is preparing the purposes of agricultural fungicides as active component.
2. purposes according to claim 1, is characterized in that Auraptenol is mixed with as active component and insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals; Or Auraptenol is mixed with as active component and other agricultural chemicals and insecticides adjuvant, excipients any one formulation allowing on agricultural chemicals.
3. purposes according to claim 2, is characterized in that described formulation is missible oil, suspending agent, aqueous emulsion, wetting powder, microemulsion or water dispersion granule; Other described agricultural chemicals are organophosphorus insecticide, pyrethroid pesticide or microbial source insecticide.
4. purposes according to claim 1, is characterized in that described insecticide is for preventing and treating Lepidoptera and Homoptera insect.
5. purposes according to claim 4, is characterized in that described lepidopterous insects is noctuid, diamond-back moth section insect, Sulfur butterfly insect.
6. purposes according to claim 5, is characterized in that described noctuid is prodenia litura, beet armyworm, mythimna separata; Described diamond-back moth section insect is diamond-back moth; Described Sulfur butterfly insect is cabbage caterpillar.
7. purposes according to claim 4, is characterized in that described Homoptera insect is Aphidiadae insect.
8. purposes according to claim 7, is characterized in that described Aphidiadae insect is black peach aphid.
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| CN102093322A (en) * | 2010-12-31 | 2011-06-15 | 厦门大学 | Application of osthole in preparation of marine antifouling paint |
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Non-Patent Citations (7)
| Title |
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| 《柑橘及其近缘属植物中天然香豆素化合物及药效作用综述》;韦献果;《食品科学》;20120715;343-347 * |
| 《蛇床子的化学成分及药理作用的研究进展》;陈艳;《沈阳药科大学学报》;20060425;256-260 * |
| bikash ranjan barik.《coumarins of murraya exotiva -absolute configuration of auraptenol》.《phytochemistry》.1983,第22卷(第3期),792-794. |
| bikash ranjan barik.《coumarins of murraya exotiva-absolute configuration of auraptenol》.《phytochemistry》.1983,第22卷(第3期),792-794. * |
| 吴寿金、赵泰等.《香豆素的结构类型》.《现代中草药成分化学》.2002,189-190. * |
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| 韦献果.《柑橘及其近缘属植物中天然香豆素化合物及药效作用综述》.《食品科学》.2012,343-347. |
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