CN104686130A - Conservation method for juncellus serotinus (rottb.) C.B. clarke - Google Patents

Conservation method for juncellus serotinus (rottb.) C.B. clarke Download PDF

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CN104686130A
CN104686130A CN201410726994.5A CN201410726994A CN104686130A CN 104686130 A CN104686130 A CN 104686130A CN 201410726994 A CN201410726994 A CN 201410726994A CN 104686130 A CN104686130 A CN 104686130A
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pond
water level
herba cyperi
rottb
clarke
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CN104686130B (en
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李苇洁
周传燕
唐金刚
史云勋
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GUIZHOU MOUNTAINOUS RESOURCES INSTITUTE
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GUIZHOU MOUNTAINOUS RESOURCES INSTITUTE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a conservation method for juncellus serotinus (rottb.) C.B. clarke. The conservation method comprises the following steps: a pond is established, juncellus serotinus (rottb.) C.B. clarke culture media are uniformly placed in the pond, then juncellus serotinus (rottb.) C.B. clarke seeds are sown in the juncellus serotinus (rottb.) C.B. clarke culture media, the seed spacing of juncellus serotinus (rottb.) C.B. clarke is 25 cm* 15 cm, water is added to the pond until the juncellus serotinus (rottb.) C.B. clarke culture media are totally soaked with water while water does not exceed the surfaces of the juncellus serotinus (rottb.) C.B. clarke culture media, the water level is kept for 25-30 days, then the water level in the pond is increased to the height 20-40 cm higher than the surfaces of the juncellus serotinus (rottb.) C.B. clarke culture media, the water level is kept for 55-60 days, and the conservation of juncellus serotinus (rottb.) C.B. clarke is finished. The pond with small water level fluctuation is established, juncellus serotinus (rottb.) C.B. clarke is planted in the pond, the water level in the pond is controlled, juncellus serotinus (rottb.) C.B. clarke acquires the most ideal water depth condition for growth and development, so that normal growth of juncellus serotinus (rottb.) C.B. clarke is maintained, the maximum underground tuber biomass is obtained, and artificial conservation of the juncellus serotinus (rottb.) C.B. clarke is realized.

Description

Herba Cyperi Glomerati nursing method
Technical field
The present invention relates to field of agricultural science and technology, especially a kind of Herba Cyperi Glomerati nursing method.
Background technology
Grass sea is maximum natural fresh water lake, Guizhou, is the most important wetlands ecosystems in the Yunnan-Guizhou Plateau, is the main migratory bird wintering ground of China and migrates one of stopover site.And sedge family Herba Cyperi Glomerati ( juncellus serotinus(Rottb.) C.B. Clarke) be the marine main emergent aquactic plant of swimming bank band of grass, except purifying water, its underground stem tuber be careless sea survive the winter the aquatic birds such as migratory bird black-necked crane, egression, heron important foodstuffs source, be the key species of the stable careless extra large ecosystem.But, in recent years the normal growth of Herba Cyperi Glomerati has been had a strong impact on to the extra large city-building of grass, has caused the flat grain of its seed, block root rot, population quantity fewer and feweri.Artificial child care is extremely urgent.At present, had the artificial child care of people to Herba Cyperi Glomerati to be studied, Tang Jingang etc. once studied the impact of variable concentrations gibberellin seed soaking on grass marine trip bank band 5 kinds of emergent aquactic plant seed sproutings, and result display germination rate is not all more than 10%.
Summary of the invention
The object of the invention is: provide a kind of Herba Cyperi Glomerati nursing method, it is simple, with low cost, and germination rate is high, to overcome the deficiencies in the prior art.
The present invention is achieved in that Herba Cyperi Glomerati nursing method, set up pond, the peat dried is mixed with the mass ratio of the mellow soil excavated in pond according to 1:3, obtain Herba Cyperi Glomerati culture matrix, evenly applied in pond by Herba Cyperi Glomerati culture matrix, its thickness is 20-30cm; By Herba Cyperi Glomerati Seeding planting in Herba Cyperi Glomerati culture matrix, water is added in pond, make water complete immersion water nutgrass flatsedge culture matrix, but be no more than the surface of Herba Cyperi Glomerati culture matrix, keep this water level after 25-30 days, improve the water level in pond, make water level higher than the surperficial 20cm of Herba Cyperi Glomerati culture matrix, keep this water level after 25-30 days, the water level in pond is increased to the surperficial 20-40cm higher than Herba Cyperi Glomerati culture matrix, keep this water level 55-60 days, namely complete the child care to Herba Cyperi Glomerati.
The seeding row spacing of Herba Cyperi Glomerati is 25cm*15cm.
1, experimental scheme and assay method
1.1 experimental scheme
Test and carry out in the Liu Jia lane of prestige Ning County grass marine swamp upstream, on 5, the pool that upstream bank band excavation specification is 300 cm × 100, cm × 200 cm, the depth of water design of Herba Cyperi Glomerati growing environment is respectively (unit: cm): 0,20,40,60,80, the pool that water level is low is excavated toward higher position, in design pool, two real-time drainings of drinking-water pipe are installed, make water level in the pool remain on design level, object prevents the destruction of rising to test.Before plantation, mixed by the mass ratio of the early stage peat dried and the mellow soil excavated in the pool by 1:3, evenly apply in each pool, thickness is about 20-30cm, rakes, with the required nutrient of Herba Cyperi Glomerati growth during guarantee test.On May 15th, 2012 plants Herba Cyperi Glomerati in each pool, and plant height is (8.48+1.20) cm.During plantation, in the pool, the depth of water only flooded native face, be considered as 0cm, according to the seeding row spacing of 25cm*15cm, Herba Cyperi Glomerati 160 strain that the plantation growth of each pool is consistent, keeps this water level to stablize to plant survival and growth always, again according to experimental scheme water level raising, mid-June, the depth of water on 4 pools was raised to 20cm simultaneously, mid-July the depth of water on 3 pools be raised to 40 respectively, 60,80cm, keep the stable of each design water level in the pool afterwards.On September 15th, 2012, by the depth of water 0,20,40,60,80cm carried out gathering measuring to test specimen, mensuration project comprises the chlorophyll content of Morphologic Parameters (plant height, base stem, leaf is long, leaf is wide), underground biomass, blade, Plant samples 30 strain is gathered under each depth of water gradient, be divided into 2 groups of equivalent at random, carry out measurement and the mensuration of Morphologic Parameters and chlorophyll content respectively, the mensuration under each depth of water gradient repeats 3 times.
1.2 assay method
Method of testing: the mensuration of Morphologic Parameters, biomass and chlorophyll content: the Morphologic Parameters of plant adopts the direct method of measurement, underground biomass adopts weight method (fresh weight, be accurate to 0.01 g), the mensuration of chlorophyll content adopts acetone ethanol extraction method, measures with U.S. Thermo ND2000c ultraviolet-uisible spectrophotometer.
Data processing: test data adopts Excel 2003 to carry out adding up and charting, adopts one-way analysis of variance (One-way ANOVA) to measure the difference between different pieces of information.
2, interpretation of result
2.1 Herba Cyperi Glomerati stems, leaf changing features
Test shows, under the different water depth gradient of 0-80cm, the rising that the long and Ye Kuanjun of the plant height of Herba Cyperi Glomerati, base stem, leaf shows as along with water level first increases rear downward trend (Fig. 1), wherein along with the further increase of the depth of water, plant height recruitment obviously reduces, the plant height recruitment of 0-20 cm is 14.97cm, 20-40cm plant height recruitment is 13.02cm; 40-60cm plant height recruitment is 5.92cm; 60-80cm plant height recruitment is-20.9cm.Base stem increasing degree under the different water depth gradient of 0-20cm is maximum, and under the depth of water of 20-60 cm, increasing degree is less, and under the 80cm depth of water, base stem have dropped 0.08cm than the 60cm depth of water.Leaf length increases 17.15cm at 0-20cm, only increases 2.29cm between 20-40cm, decreases 7.3cm between 40-60cm, and 60-80cm increases 1.49.Leaf is wide is increase trend between 0-60cm, but 0-20 increasing degree is comparatively large, and 80cm have dropped 0.06 than 60cm.One-way analysis of variance (One-way ANOVA) result shows, under each depth of water gradient, Herba Cyperi Glomerati plant height, base stem, leaf difference that is long, wide of leaf do not reach significance level (p > 0.05).Visible, along with the rising of water level, in the scope that it can tolerate, Herba Cyperi Glomerati is that the biomass by increasing the ground such as plant height, leaf length, Ye Kuan increases and the contact surface in the external world, to obtain the necessary energy of growth, this is the self-adaptation of plant to environmental change instinct, but this tolerance is within the specific limits, when the depth of water is more than 60cm, there is downward trend in this regulating power, plant height, leaf length, Ye Kuan etc. no longer increase, and illustrate that the depth of water now has exceeded the tolerance range of plant.
2.2 chlorophyll content changes
Under different water depth gradient, as shown in Figure 2, within the scope of 0-60cm hydraulic gradiant, along with the rising of water level, chlorophyll content is totally in increase trend for the difference between Herba Cyperi Glomerati chlorophyll content in leaf blades.One-way analysis of variance show this species diversity do not reach significance level ( p>0.05), be the highest during 40cm water level, reach (1.912 ± 0.146) mg.g -1, and be minimum during 80cm water level, for the content of 64.4%. chlorophyll a during 40cm water level is totally higher than chlorophyll b (exceeding about 278.7%), and both and chlorophyll content have identical variation tendency, and minimum is respectively 63.3% and 64.4% of peak.2 results and analysis figure figure
2.3 underground biomass changes
As can be seen from the change (Fig. 3) of different water level biomass, 0-60cm hydraulic gradiant test time, the underground biomass of Herba Cyperi Glomerati all increases along with the rising of water level, and the difference between different water level reach significance level ( p<0.05), during its middle water level 20cm, stem tuber mean fresh in underground is 12.31g, is the maximum of all test depth of water, exceeds 27.04% than 0 water level, exceed 82.11% than the 80cm depth of water, but the underground stem tuber weight differential of 40cm and the 60cm depth of water is less.As can be seen from plant height, leaf is long, leaf is wide analysis, along with the rising of water level, ground biomass then has larger difference, visible, Herba Cyperi Glomerati is by regulating the allocation proportion of ground biomass and underground biomass to adapt to the water level constantly raised, when water level is more than 20cm, in order to increase the contact area with air, improve the acquisition speed of oxygen, reduce the consumption of breathing by reducing underground biomass, this is that plant is to the self-control of adverse circumstance and adaptation.Water level can be worked as when being increased to 80cm, underground biomass presents and significantly declines, be only 54.31% during 20cm water level, and there is putrefactive phenomenon in most stem tuber, visible, depth of water 60cm is a threshold value of Herba Cyperi Glomerati growing environment, more than 60cm, its plant height, leaf are long, leaf is wide no longer in the trend increased, but have the decline of a large scale.Herba Cyperi Glomerati is applicable to shallow water area growth in a word, is advisable, does not the highlyest exceed 60cm with the 20-40cm depth of water.
3 conclusions and discussion
1) in the depth of water graded of 0-60cm, Herba Cyperi Glomerati plant height, leaf length, Ye Kuan, base stem and subterranean stem fresh weight increase all to some extent, and as water level 20cm, this increase is the most remarkable, and during more than 40cm, increasing degree slowly reduces.When the depth of water is more than 60cm, Herba Cyperi Glomerati growth is subject to serious impact, and every analysis indexes all shows reduction in various degree, is exactly the most significantly wherein that underground stem tuber rots and weight reduction.Visible, Herba Cyperi Glomerati loses the ability of self-adaptation under high water level environment, and it is more suitable for the depth of water environment of below 60cm.
2) in the depth of water graded of 0-60cm, chlorophyll content is totally in increase trend, embody it, within the scope of the water depth ratio of 0-60cm, there is certain tolerance, when the depth of water is more than 60cm, chlorophyll content presents downward trend, and it grows the impact received to a certain degree, finally causes yellow leaf, plant is short and small, and underground tuber biomass reduces and rots.
3) found by above result of the test, Herba Cyperi Glomerati is suitable at shallow water area growth and breeding, and 20-40cm is its optimum growth water level, and Herba Cyperi Glomerati population quantity within the scope of this depth of water is comparatively large, grows more prosperous.But when water level rises again, it also has certain tolerance, the threshold value of this tolerance is 60cm.
4) should select at upstream bank band the zone design plantation pool that water-level fluctuation is less, be suitable for Herba Cyperi Glomerati growth for Herba Cyperi Glomerati child care, Artificial Control water level (20cm≤H≤40cm), to reach the object of child care.
Owing to have employed technique scheme, compared with prior art, the present invention sets up the little pond of water-level fluctuation, in pond, plant Herba Cyperi Glomerati, control the water level in pond, make Herba Cyperi Glomerati obtain its optimal depth condition that grows, thus maintain its normal growth, obtain maximum underground tuber biomass, realize the artificial child care of Herba Cyperi Glomerati, for the extra large Wintering area of grass provides more food.The present invention is simple, with low cost, and result of use is good.
Accompanying drawing explanation
Fig. 1 is that Herba Cyperi Glomerati plant height, base stem, leaf are long, the wide change with the depth of water of leaf;
Fig. 2 is the leaf green changes of contents of Herba Cyperi Glomerati;
Fig. 3 is underground stem block weight.
Embodiment
Embodiments of the invention 1: Herba Cyperi Glomerati nursing method, in careless marine swamp upstream, bank band higher position sets up pond, the peat dried is mixed with the mass ratio of the mellow soil excavated in pond according to 1:3, obtain Herba Cyperi Glomerati culture matrix, evenly applied in pond by Herba Cyperi Glomerati culture matrix, its thickness is 25cm; Mid-May, by Herba Cyperi Glomerati Seeding planting in Herba Cyperi Glomerati culture matrix, the seeding row spacing of Herba Cyperi Glomerati is 25cm*15cm, water is added in pond, make water complete immersion water nutgrass flatsedge culture matrix, but be no more than the surface of Herba Cyperi Glomerati culture matrix, keep this water level after 30 days, improve the water level in pond, make water level higher than the surperficial 20cm of Herba Cyperi Glomerati culture matrix, keep this water level after 30 days, the water level in pond is increased to the surperficial 40cm higher than Herba Cyperi Glomerati culture matrix, keep this water level 60 days, namely complete the child care to Herba Cyperi Glomerati.
Embodiments of the invention 2: Herba Cyperi Glomerati nursing method, in careless marine swamp upstream, bank band higher position sets up pond, the peat dried is mixed with the mass ratio of the mellow soil excavated in pond according to 1:3, obtain Herba Cyperi Glomerati culture matrix, evenly applied in pond by Herba Cyperi Glomerati culture matrix, its thickness is 20cm; Mid-May, by Herba Cyperi Glomerati Seeding planting in Herba Cyperi Glomerati culture matrix, the seeding row spacing of Herba Cyperi Glomerati is 25cm*15cm, water is added in pond, make water complete immersion water nutgrass flatsedge culture matrix, but be no more than the surface of Herba Cyperi Glomerati culture matrix, keep this water level after 30 days, improve the water level in pond, make water level higher than the surperficial 20cm of Herba Cyperi Glomerati culture matrix, keep this water level after 25 days, the water level in pond is increased to the surperficial 30cm higher than Herba Cyperi Glomerati culture matrix, keep this water level 55 days, namely complete the child care to Herba Cyperi Glomerati.
Embodiments of the invention 3: Herba Cyperi Glomerati nursing method, in careless marine swamp upstream, bank band higher position sets up pond, the peat dried is mixed with the mass ratio of the mellow soil excavated in pond according to 1:3, obtain Herba Cyperi Glomerati culture matrix, evenly applied in pond by Herba Cyperi Glomerati culture matrix, its thickness is 30cm; Mid-May, by Herba Cyperi Glomerati Seeding planting in Herba Cyperi Glomerati culture matrix, the seeding row spacing of Herba Cyperi Glomerati is 25cm*15cm, in pond, add water, makes water complete immersion water nutgrass flatsedge culture matrix, but be no more than the surface of Herba Cyperi Glomerati culture matrix, keep this water level after 25 days, improve the water level in pond, make water level higher than the surperficial 20cm of Herba Cyperi Glomerati culture matrix, keep this water level 90 days, namely complete the child care to Herba Cyperi Glomerati.

Claims (2)

1. a Herba Cyperi Glomerati nursing method, it is characterized in that: set up pond, the peat dried is mixed with the mass ratio of the mellow soil excavated in pond according to 1:3, obtain Herba Cyperi Glomerati culture matrix, evenly applied in pond by Herba Cyperi Glomerati culture matrix, its thickness is 20-30cm; Then by Herba Cyperi Glomerati Seeding planting in Herba Cyperi Glomerati culture matrix, water is added in pond, make water complete immersion water nutgrass flatsedge culture matrix, but be no more than the surface of Herba Cyperi Glomerati culture matrix, keep this water level after 25-30 days, improve the water level in pond, make water level higher than the surperficial 20cm of Herba Cyperi Glomerati culture matrix, keep this water level after 25-30 days, the water level in pond is increased to the surperficial 20-40cm higher than Herba Cyperi Glomerati culture matrix, keep this water level 55-60 days, namely complete the child care to Herba Cyperi Glomerati.
2. Herba Cyperi Glomerati nursing method according to claim 1, is characterized in that: the field planting seeding row spacing of Herba Cyperi Glomerati is 25cm*15cm.
CN201410726994.5A 2014-12-04 2014-12-04 Conservation method for juncellus serotinus (rottb.) C.B. clarke Expired - Fee Related CN104686130B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113742945A (en) * 2021-09-30 2021-12-03 中国科学院东北地理与农业生态研究所 Optimization method for optimal water level of cattail growth in soda saline-alkali wetland

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1350777A (en) * 2000-10-27 2002-05-29 金东春 Culture of cyperus
CN101759296A (en) * 2009-12-30 2010-06-30 昆明理工大学 Courtyard-style landscape wetland
CN101838053A (en) * 2009-03-20 2010-09-22 宝山钢铁股份有限公司 Method for constructing artificial wetland through aged refuse for processing sewage
CN102060386A (en) * 2010-11-17 2011-05-18 中国科学院南京地理与湖泊研究所 Method for constructing floating wetland
CN102491525A (en) * 2011-12-07 2012-06-13 中国科学院武汉植物园 Method for building river course ecological restoration vegetation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1350777A (en) * 2000-10-27 2002-05-29 金东春 Culture of cyperus
CN101838053A (en) * 2009-03-20 2010-09-22 宝山钢铁股份有限公司 Method for constructing artificial wetland through aged refuse for processing sewage
CN101759296A (en) * 2009-12-30 2010-06-30 昆明理工大学 Courtyard-style landscape wetland
CN102060386A (en) * 2010-11-17 2011-05-18 中国科学院南京地理与湖泊研究所 Method for constructing floating wetland
CN102491525A (en) * 2011-12-07 2012-06-13 中国科学院武汉植物园 Method for building river course ecological restoration vegetation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113742945A (en) * 2021-09-30 2021-12-03 中国科学院东北地理与农业生态研究所 Optimization method for optimal water level of cattail growth in soda saline-alkali wetland

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