CN105104046A - Method of melatonin induction processing for improving resistance to rice bacterial leaf blight - Google Patents
Method of melatonin induction processing for improving resistance to rice bacterial leaf blight Download PDFInfo
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- CN105104046A CN105104046A CN201510565734.9A CN201510565734A CN105104046A CN 105104046 A CN105104046 A CN 105104046A CN 201510565734 A CN201510565734 A CN 201510565734A CN 105104046 A CN105104046 A CN 105104046A
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- 230000006698 induction Effects 0.000 title claims abstract description 16
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 title abstract description 8
- 229960003987 melatonin Drugs 0.000 title abstract description 8
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a method of melatonin induction processing for improving resistance to rice bacterial leaf blight, which comprises following steps: during the adult stage, the rice is irrigated with melatonin solution of 50 to 500 mu mol/L of concentration, for 5 to 10 days. According to the invention, the method is feasible; the operation is convenient; the investment cost is low and the effect is rapid; and the resistance performance of the rice to the bacterial leaf blight can be improved in short term, thus increasing the economy benefit of the rice during the outbreak of bacterial leaf blight.
Description
Technical field
The invention belongs to paddy disease-resistant research field, be specifically related to a kind of method being improved rice bacterial blight resistance by short time epiphysin process, the method is applicable to any plantation of regional paddy rice of China and the paddy growth of bacterial blight-resisting.
Background technology
Plant is often faced with infecting of miscellaneous pathogenic microorganism, and these disease infestation are inevitable unfavorable factors in plant life history.But, plant can not be moved due to self, stress from outside can only be replied when suffering adverse circumstance environment, thus with the long-term coevolution process of pathogenic microorganism, the regulatory mechanism of series of complex and the precision of evolving out, experience outside to coerce and transmission of signal, thus infecting of most of pathogenic microorganism can be resisted.The thicker cuticula that the cell structure of one side plant corpus itself and cell wall comprise and lignin etc. can resist infecting of external pathogen to a certain extent; On the other hand when external pathogen infection plant, can usually be induced in plant corpus to produce hypersensitivity, by the position of pathogen infection by producing hypersensitivity inducing cell death, to stop pathogen infecting further plant, infecting the position that plant corpus also can be made not infected and can produce systemic acquired resistance simultaneously due to bacterium.
Disease resistance of plant is the result of a series of Defense response gene coordinate expression, and transcriptional control is the major way of plant defense gene expression regulation, and it can determine the disease-resistant or susceptible degree that infect of plant to pathogen.The transcriptional control of plant disease-resistant defense response relates to the effect of numerous transcription factor.After plant experiences invasion pathogen signal, first experience by the inductor on plant cell membrane, then signal is transmitted to downstream and causes the generation of second messenger, after a series of signal transmission event, transcription factor is activated, the transcription factor of activation is done mutually with the specificity of the cis-acting elements generation albumen in corresponding gene promotor and DNA in cell nucleus, thus activates the transcriptional expression of defense response related gene.
Paddy rice is one of important crops of depending on for existence of the mankind, generally cultivates in the coastal plain in the areas such as the torrid zone, subtropics and temperate zone, the waterflooding of tidal delta and river basin.Bacterial leaf-blight is one of Major Diseases of paddy rice, comes into being yellow green spot in diseased plant blade tip and edge, develops into pale asphyxia, the rectangular spot of yellowish-brown along vein afterwards, finally becomes canescence and withered.Diseased plant easily lodges, and the unreal rate of spike of rice increases.Germ survives the winter propagation on seed and ill straw.Tillering stage, disease started development.High temperature and humidity, storm, rice field by flood and nitrogenous fertilizer too much time be conducive to plant disease epidemic.Bacterial blight of rice all has generation in Ge Dao district of China, comparatively large to yield effect, not plump paddy and crack rice many, and the underproduction reaches 20%-30%, and heavy reached at 50%-60%, even No kernels or seeds are gathered, as in a year of scarcity.The mechanism of Study On Rice bacterial blight-resisting, thus the various measure that can improve rice bacterial blight resistance ability is provided or directly cultivates disease-resistant variety, obviously significant.
Melatonin is human brain depths a kind of amine bormones that size " pineal gland " is secreted as pine nut, so there is people to be its " epiphysin ".Melatonin is the hormone be secreted by the pineal gland being positioned at diacele rear wall.The maximum feature of epiphysin should be that it is the strongest endogenous free radical scavenger found so far.The basic function of epiphysin participates in antioxidant system exactly, and prevent cell from producing oxidative damage, in this respect, its effect has exceeded known all substance in vivos, and the activity of various endocrine gland in its control volume.At present, both at home and abroad to the biological function of melatonin, the health care especially as dietary supplements conducts extensive research, show its have promote sleep, regulate the time difference, anti-ageing, immunity moderation, the multinomial physiological function such as antitumor.Recent study finds also also particularly to have eaten in various plants containing melatonin in plant and detected in medicinal plant, and the nutrition to the mankind, medicine and agricultural are provided the information be highly profitable by the research therefore extensively carrying out melatonin in plant.
Summary of the invention
The present inventor, in the test of Study On Rice bacterial blight-resisting mechanism for many years, is surprised to find that the epiphysin induction process by low concentration can improve the characteristic of rice bacterial blight resistance.
Therefore, the object of the present invention is to provide a kind of method improving rice bacterial blight resistance, the method is easy, easy and simple to handle, takes effect quick, can improve the resistance of paddy rice to bacterial leaf-blight in a short time, improve the economic benefit of paddy rice when bacterial leaf-blight breaks out.
In order to realize above-mentioned goal of the invention, technical scheme overview of the present invention is as follows:
Epiphysin induction process improves a method for rice bacterial blight resistance, and the method comprises the steps: to pour to the paddy rice being in Adult plant the epiphysin solution that concentration is 50-500 μm of ol/L continuously, and the time is 5-10d.
It should be noted that, before epiphysin induction process paddy rice, also need to do following routine work:
(1) planting site preparation: the mechanism utilizing plough, rake, in conjunction with agrotechnique such as fertilising, irrigation and efficent rotations, make rice field smooth, Tanaka is without accumulated water, and dry field degree is consistent, is beneficial to paddy growth neat; Make rice field soil block in small, broken bits, clay fertilizer melts mutually, and topsoil is soft, without trash simultaneously.Then add base fertilizer, every mu adds composite fertilizer's (containing potassium sulfate and nitrate nitrogen, general total nutrient is not less than 45%) of about 50 kilograms, finally flattens bed surface.
(2) seedling management: first can cultivate rice seedling certain block Tanaka, after planting to 1 leaf dew point, temperature is based on insulation, and temperature is at 25-30 DEG C, and 2 leaf phases kept 25 DEG C, and 3 leaf phases kept 20-22 DEG C.Nighttime temperature is on the low side, should take in booth, to open the measure such as electric light or some burning rice husk to heat, at the outer cover straw of canopy or cold-proofly will being heated by the measure of grade of hut nursery, guarantees to reach the minimum temperature index needed for each leaf age of rice shoot, makes seed rice can normal growth growth on seedbed.When rice shoot grows tall about 8cm, by manual pull up seedlings rice transplanting, rice transplanting, the machine transplanting of rice etc., the fair weather of cold tail is selected to start the work of transplanting; Rice transplanting will be accomplished shallow, even, straight, steady, and transplant depth half is no more than 3 centimetres, and accomplishes that rice shoot band medicine, band are fertile, band soil.At the nursery initial stage, suitably water, after 40 days, irrigate according to natural situation rainwater.
Further preferably, epiphysin induction process described above improves the method for rice bacterial blight resistance, wherein starts Rice Seedling Height 40-60cm when pouring epiphysin solution.
Further preferably, epiphysin induction process described above improves the method for rice bacterial blight resistance, and the concentration of epiphysin solution is wherein 100-500 μm of ol/L, and every mu pours epiphysin solution 800-1200L.
Further preferably, epiphysin induction process described above improves the method for rice bacterial blight resistance, and the time wherein pouring epiphysin solution is continuously 7d, 1 time/d.
By epiphysin solution 100 or the process of 500 μm of ol/L (namely containing 0.023g or 0.116g epiphysin in every premium on currency) of above short time (7 days) low concentration, find that treated water rice material ratio does not have treated water rice material growing state when bacterial leaf-blight infects good, show as scab length on blade shorter, more disease-resistant.As shown in Figure 1, after with the epiphysin solution 100 of low concentration or 500 μm of ol/L (namely in every premium on currency containing 0.023g or 0.116g epiphysin) pretreatment, bacterial leaf-blight infected rice leaf after 15 days, 72%-79% when scab length is epiphysin pretreatment useless on rice leaf.Based on above-mentioned achievement in research, present invention also offers the purposes of epiphysin in paddy disease-resistant Sexual Physiology inductive technology, that is: epiphysin is improving the application in rice bacterial blight resistance.
Compared with prior art, the present invention has the following advantages and progressive: the method is easy, easy and simple to handle, and input cost is low, instant effect, can improve the resistance of paddy rice to bacterial leaf-blight in a short time, thus increases the economic benefit of paddy rice when bacterial leaf-blight breaks out.
Accompanying drawing explanation
Fig. 1 a kind ofly waters variable concentrations epiphysin solution induction process 7 days continuously and affects schematic diagram to rice bacterial blight resistance;
The rice material of normal growth is divided into three parts, first part is normally watered running water 7 days, second part is watered 100 μm of ol/L epiphysin solution (namely containing 0.023g epiphysin in every premium on currency) 7 days, and the 3rd part is watered 500 μm of ol/L epiphysin solution (namely containing 0.116g epiphysin in every premium on currency) 7 days.Then get the rice leaf of these three parts of materials, pick bacterial leaf spot bacterium liquid (OD with scissors
600=0.5) cut rice leaf, the consistent plant of 20 strain growing ways inoculated by each material, and the leaf of 3 full extension is cut in every strain, infecting 5 days, 10 days and 15 days time investigation scab length.
Embodiment
Be below the specific embodiment that the present invention relates to, technical scheme of the present invention is described further, but protection scope of the present invention is not limited to this embodiment.Every do not deviate from the present invention's design change or equivalent substituting include within protection scope of the present invention.
Embodiment 1: epiphysin induction process improves the experimental study of rice bacterial blight resistance:
1, planting site prepares:
Utilize plough, the mechanism of rake, in conjunction with agrotechnique such as fertilising, irrigation and efficent rotations, make rice field smooth, Tanaka is without accumulated water, and dry field degree is consistent, is beneficial to paddy growth neat; Make rice field soil block in small, broken bits, clay fertilizer melts mutually, and topsoil is soft, without trash simultaneously.Then add base fertilizer, every mu adds composite fertilizer's (containing potassium sulfate and nitrate nitrogen, general total nutrient is not less than 45%) of 50 kilograms, finally flattens bed surface.
2, seedling management:
First cultivate rice seedling certain block Tanaka, after planting to 1 leaf dew point, temperature is based on insulation, and optimum temperature 25-28 DEG C, 2 leaf phases kept 25 DEG C, and 3 leaf phases kept 20-22 DEG C.Nighttime temperature is on the low side, should take in booth, to open the measure such as electric light or some burning rice husk to heat, at the outer cover straw of canopy or cold-proofly will being heated by the measure of grade of hut nursery, guarantees to reach the minimum temperature index needed for each leaf age of rice shoot, makes seed rice can normal growth growth on seedbed.Rice shoot grow tall about eight centimeters time, by manual pull up seedlings rice transplanting, rice transplanting, the machine transplanting of rice etc., select the fair weather of cold tail to start the work of transplanting; Rice transplanting will be accomplished shallow, even, straight, steady, and transplant depth half is no more than 3 centimetres, and accomplishes that rice shoot band medicine, band are fertile, band soil.At the nursery initial stage, suitably water, after 40 days, irrigate according to natural situation rainwater.
3, epiphysin induction process:
Choose the paddy rice (height is 50cm about) of Adult plant, continuous epiphysin solution 100 or 500 μm of ol/L (namely containing 0.023g or 0.116g epiphysin in every premium on currency) [every mu of 1000L epiphysin solution that waters (altogether containing 23g or 116g epiphysin in 1000L epiphysin solution)] of within 7 days, watering low concentration, once a day.In other times, water according to normal condition, i.e. the every mu of 1500L that waters; After 40 days, irrigate according to natural situation rainwater.
4, paddy rice is to the Analysis of Resistance of bacterial leaf-blight:
First at PSA solid culture medium (peptone 10g/L; Sucrose 10g/L; Glutamic acid 1g/L; PH6.8-7.0; Every 1L adds agar powder 20g) upper 28 DEG C of activation pathogen, i.e. Xanthomonas oryzae strain X oo-PXO99, picking list bacterium colony, with PSA liquid nutrient medium (peptone 10g/L; Sucrose 10g/L; Glutamic acid 1g/L; PH6.8-7.0) 180r/min shakes bacterium propagation.Get bacterium liquid after 1 day to rule on PSA solid culture medium again, light culture 2 days, then with sterile water, bacterium is washed from medium, bacterial concentration is transferred to OD for 28 DEG C
600=0.5.Adopt the paddy rice of leaf-cutting method to Adult plant to inoculate, method is as follows: choose rice leaf inoculation, pick bacterial leaf spot bacterium liquid (OD with scissors
600=0.5) cut rice leaf, the plant that each material inoculation 10-20 strain growing way is consistent, the leaf of 3 full extension is cut in every strain, is infecting 5 days, 10 days and 15 days time investigate scab length.
By epiphysin solution 100 or the process of 500 μm of ol/L (namely containing 0.023g or 0.116g epiphysin in every premium on currency) of above short time (7 days) low concentration, find that treated water rice material ratio does not have treated water rice material growing state when bacterial leaf-blight infects good, show as scab length on blade shorter, more disease-resistant.Specifically as shown in Figure 1, after with the epiphysin solution 100 of low concentration or 500 μm of ol/L (namely in every premium on currency containing 0.023g or 0.116g epiphysin) pretreatment, when bacterial leaf-blight infects rice leaf 15 days, 72%-79% when scab length is epiphysin pretreatment useless on rice leaf.
The inventive method is easy, easy and simple to handle, takes effect quick, can improve the resistance of paddy rice to bacterial leaf-blight in a short time, improve the economic benefit of paddy rice when bacterial leaf-blight breaks out.
Claims (5)
1. epiphysin induction process improves a method for rice bacterial blight resistance, and it is characterized in that, the method comprises the steps: to pour to the paddy rice being in Adult plant the epiphysin solution that concentration is 50-500 μm of ol/L continuously, and the time is 5-10d.
2. epiphysin induction process improves the method for rice bacterial blight resistance according to claim 1, it is characterized in that, starts Rice Seedling Height 40-60cm when pouring epiphysin solution.
3. epiphysin induction process improves the method for rice bacterial blight resistance according to claim 1, and it is characterized in that, the concentration of described epiphysin solution is 100-500 μm of ol/L, and every mu pours epiphysin solution 800-1200L.
4. epiphysin induction process improves the method for rice bacterial blight resistance according to claim 1, and it is characterized in that, the time pouring epiphysin solution is continuously 7d, 1 time/d.
5. epiphysin is improving the application in rice bacterial blight resistance.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107156128A (en) * | 2017-06-09 | 2017-09-15 | 江苏省农业科学院 | A kind of utilization epiphysin improves method of the paddy rice to strip virus resistance |
| CN108013037A (en) * | 2017-12-15 | 2018-05-11 | 江西农业大学 | A kind of method using epiphysin increase female flower of cucumber rate |
| CN108684677A (en) * | 2018-05-08 | 2018-10-23 | 江苏省农业科学院 | Application of the epiphysin in preventing and treating bacterial leaf streak of rice |
| CN109479689A (en) * | 2018-12-14 | 2019-03-19 | 扬州大学 | A kind of epiphysin efficiently concentrating method in Vegetables in Brassica sprout |
| CN110024611A (en) * | 2019-05-24 | 2019-07-19 | 海南大学 | A kind of betel nut alkali process improves the method and application of betel nut disease resistance |
| CN110326477A (en) * | 2019-07-12 | 2019-10-15 | 中国科学院合肥物质科学研究院 | A method of wheat is improved to powder mildew resistance |
| CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
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| CN201450921U (en) * | 2009-08-26 | 2010-05-12 | 云南省农业科学院生物技术与种质资源研究所 | Special inoculation device for rice bacterial leaf blight |
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| CN108684677A (en) * | 2018-05-08 | 2018-10-23 | 江苏省农业科学院 | Application of the epiphysin in preventing and treating bacterial leaf streak of rice |
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| CN109479689B (en) * | 2018-12-14 | 2021-08-24 | 扬州大学 | Method for efficiently enriching melatonin in brassica vegetable sprouts |
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| CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
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