CN104450651A - 一种β-葡萄糖苷酶突变体及其应用 - Google Patents
一种β-葡萄糖苷酶突变体及其应用 Download PDFInfo
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- CN104450651A CN104450651A CN201410753327.6A CN201410753327A CN104450651A CN 104450651 A CN104450651 A CN 104450651A CN 201410753327 A CN201410753327 A CN 201410753327A CN 104450651 A CN104450651 A CN 104450651A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
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Abstract
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CN201410753327.6A CN104450651B (zh) | 2014-12-09 | 2014-12-09 | 一种β‑葡萄糖苷酶突变体及其应用 |
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CN201410753327.6A CN104450651B (zh) | 2014-12-09 | 2014-12-09 | 一种β‑葡萄糖苷酶突变体及其应用 |
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CN104450651A true CN104450651A (zh) | 2015-03-25 |
CN104450651B CN104450651B (zh) | 2017-08-15 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104975039A (zh) * | 2015-06-24 | 2015-10-14 | 方诩 | 一种重组质粒及其在降解纤维素原料中的应用 |
CN105524903A (zh) * | 2016-01-28 | 2016-04-27 | 中国农业科学院饲料研究所 | 一种β-葡萄糖苷酶改良突变体E168Q及其编码基因和应用 |
CN105567663A (zh) * | 2016-01-28 | 2016-05-11 | 中国农业科学院饲料研究所 | 一种催化效率提高的β-葡萄糖苷酶突变体F67Y及其编码基因和应用 |
CN105567662A (zh) * | 2016-01-28 | 2016-05-11 | 中国农业科学院饲料研究所 | 一种嗜热β-葡萄糖苷酶突变体-M36N及其编码基因和应用 |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
CN117402858A (zh) * | 2023-12-13 | 2024-01-16 | 北京科为博生物科技有限公司 | 一种耐热性提高的β-葡萄糖苷酶突变体 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004099228A2 (en) * | 2003-05-02 | 2004-11-18 | Novozymes Inc. | Variants of beta-glucosidases |
CN103589701A (zh) * | 2013-11-18 | 2014-02-19 | 青岛蔚蓝生物集团有限公司 | 一种低温纤维素酶及其应用 |
CN103642778A (zh) * | 2013-12-18 | 2014-03-19 | 青岛蔚蓝生物集团有限公司 | 一种β-内切葡聚糖酶突变体及其应用 |
CN103725660A (zh) * | 2013-12-04 | 2014-04-16 | 青岛蔚蓝生物集团有限公司 | 一种内切葡聚糖酶及其应用 |
CN103865905A (zh) * | 2014-03-13 | 2014-06-18 | 青岛蔚蓝生物集团有限公司 | 一种内切葡聚糖酶 |
-
2014
- 2014-12-09 CN CN201410753327.6A patent/CN104450651B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004099228A2 (en) * | 2003-05-02 | 2004-11-18 | Novozymes Inc. | Variants of beta-glucosidases |
CN103589701A (zh) * | 2013-11-18 | 2014-02-19 | 青岛蔚蓝生物集团有限公司 | 一种低温纤维素酶及其应用 |
CN103725660A (zh) * | 2013-12-04 | 2014-04-16 | 青岛蔚蓝生物集团有限公司 | 一种内切葡聚糖酶及其应用 |
CN103642778A (zh) * | 2013-12-18 | 2014-03-19 | 青岛蔚蓝生物集团有限公司 | 一种β-内切葡聚糖酶突变体及其应用 |
CN103865905A (zh) * | 2014-03-13 | 2014-06-18 | 青岛蔚蓝生物集团有限公司 | 一种内切葡聚糖酶 |
Non-Patent Citations (1)
Title |
---|
RIOU.CHRISTINE ET AL: "purification,characterization and substrate specificity ofanovel highly glucose tolerant beta-glucosidasefromaspergillus oryzae", 《APPL ENVIRON. MICROBIOL.》 * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104975039A (zh) * | 2015-06-24 | 2015-10-14 | 方诩 | 一种重组质粒及其在降解纤维素原料中的应用 |
CN104975039B (zh) * | 2015-06-24 | 2018-05-11 | 方诩 | 一种重组质粒及其在降解纤维素原料中的应用 |
CN105524903A (zh) * | 2016-01-28 | 2016-04-27 | 中国农业科学院饲料研究所 | 一种β-葡萄糖苷酶改良突变体E168Q及其编码基因和应用 |
CN105567663A (zh) * | 2016-01-28 | 2016-05-11 | 中国农业科学院饲料研究所 | 一种催化效率提高的β-葡萄糖苷酶突变体F67Y及其编码基因和应用 |
CN105567662A (zh) * | 2016-01-28 | 2016-05-11 | 中国农业科学院饲料研究所 | 一种嗜热β-葡萄糖苷酶突变体-M36N及其编码基因和应用 |
CN105567663B (zh) * | 2016-01-28 | 2018-09-07 | 中国农业科学院饲料研究所 | 一种催化效率提高的β-葡萄糖苷酶突变体F67Y及其编码基因和应用 |
CN105567662B (zh) * | 2016-01-28 | 2018-09-07 | 中国农业科学院饲料研究所 | 一种嗜热β-葡萄糖苷酶突变体-M36N及其编码基因和应用 |
CN105524903B (zh) * | 2016-01-28 | 2019-03-12 | 中国农业科学院饲料研究所 | 一种β-葡萄糖苷酶改良突变体E168Q及其编码基因和应用 |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
CN117402858A (zh) * | 2023-12-13 | 2024-01-16 | 北京科为博生物科技有限公司 | 一种耐热性提高的β-葡萄糖苷酶突变体 |
CN117402858B (zh) * | 2023-12-13 | 2024-03-15 | 北京科为博生物科技有限公司 | 一种耐热性提高的β-葡萄糖苷酶突变体 |
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CN104450651B (zh) | 2017-08-15 |
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Inventor after: Zhang Yiman Inventor after: Zhang Qing Inventor after: Li Bin Inventor after: Cao Tishuang Inventor after: Dong Jiqiao Inventor after: Tang Bo Inventor after: Huang Yijun Inventor after: Wang Huaming Inventor before: Zhang Qing Inventor before: Li Bin Inventor before: Cao Tishuang Inventor before: Dong Jiqiao Inventor before: Tang Bo Inventor before: Huang Yijun Inventor before: Wang Huaming |
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