CN104522358A - Chinese herbal medicine microbial ecological agent for improving weight of weaned pig and preparation method of Chinese herbal medicine microbial ecological agent - Google Patents
Chinese herbal medicine microbial ecological agent for improving weight of weaned pig and preparation method of Chinese herbal medicine microbial ecological agent Download PDFInfo
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Abstract
The invention discloses a Chinese herbal medicine microbial ecological agent for improving weight of a weaned pig and a preparation method of the Chinese herbal medicine microbial ecological agent. The Chinese herbal medicine microbial ecological agent is prepared from the following raw materials in parts by weight: 6-15 parts of astragalus membranaceus, 5-10 parts of angelica sinensis, 5-15 parts of poria cocos, 5-10 parts of roots of Chinese pulsatilla, 30-40 parts of a lactobacillus liquid, 30-40 parts of a saccharomycete liquid and 30-40 parts of a bacillus subtilis liquid. The Chinese herbal medicine microbial ecological agent prepared by the technical scheme disclosed by the invention can be used for effectively improving the immunity of the body of the weaned pig, preventing piglet diarrhea and promoting digestive absorption of nutritional matters so as to realize the purpose of preventing and treating diseases and improving the production performance; the Chinese herbal medicine microbial ecological agent can be used for improving the weight of the weaned pig and avoiding medicinal residues and environmental pollution caused by adding chemical medicines and is efficient and free of toxic and side effects and residues.
Description
Technical field
The present invention relates to a kind of animal feed additive, be specifically related to a kind ofly improve Chinese herbal microecological preparation of weanling pig weight and preparation method thereof.
Background technology
After weaned piglet, due to the impact by the factor such as feed, environmental stress, easily cause piglet apocleisis, diarrhea, growth retardation, weightlessness, thus cause the death rate of piglet to account for about 85% of the whole growth phase death rate.In the past, main employing in feed added antibiotic method raising livestock and poultry daily gain and feed efficiency, reduced the death rate.But generally while use antibiotic suppresses pathogenic bacteria, also murdered microorganism useful in animal body, destroyed microecological balance, result turn increases the generation of disease of digestive tract on the contrary.In addition, along with expanding gradually of antibiotic application and going deep into of studying, it is found that Long-Time Service antibiotic can force pathogenic microorganisms to morph, produce drug resistance strain, whole food chain is produced and pollutes, thus cause a series of the problem of the safety of of stockbreeding production, directly threaten the health of the mankind.
Summary of the invention
The object of the invention is the defect for prior art, a kind of Chinese herbal microecological preparation improving weanling pig weight is provided, additionally provide a kind of method preparing the Chinese herbal microecological preparation improving weanling pig weight.
In order to realize above object, the technical solution adopted in the present invention is:
A kind of Chinese herbal microecological preparation improving weanling pig weight, be made up of the raw material of following weight portion: the Radix Astragali 6 ~ 15 parts, Radix Angelicae Sinensis 5 ~ 10 parts, 5 ~ 15 parts, Poria cocos, the Chinese bulbul 5 ~ 10 parts, Bacillus acidi lactici bacterium liquid 30 ~ 40 parts, saccharomycete bacterium liquid 30 ~ 40 parts and bacillus subtilis bacterium liquid 30 ~ 40 parts.
Improve a Chinese herbal microecological preparation for weanling pig weight, be made up of the raw material of following weight portion: the Radix Astragali 12 parts, Radix Angelicae Sinensis 8 parts, 12 parts, Poria cocos, the Chinese bulbul 8 parts, Bacillus acidi lactici bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 40 parts.
Improve a preparation method for the Chinese herbal microecological preparation of weanling pig weight, concrete steps are as follows:
(1) take the Radix Astragali, Radix Angelicae Sinensis, Poria cocos, the Chinese bulbul, pulverize after drying, cross 80 ~ 100 mesh sieves for subsequent use;
(2) extracting lactic acid bacillus bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid add in the herbal powder of step (1) gained respectively, employing closing anaerobic ferments, fermentation temperature is 30 DEG C ~ 37 DEG C, fermentation time is 36 ~ 72 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation.
Wherein, the bake out temperature in step (1) is 60 ~ 80 DEG C.
Step (2) described Bacillus acidi lactici bacterium liquid is prepared by following methods: Lactobacillus species is carried out slant strains cultivation on MRS solid medium, adds the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension in cultured slant strains; The bacteria suspension produced being cultivated on inclined-plane in MRS fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, Bacillus acidi lactici bacterium liquid is obtained.
Step (2) described saccharomycete bacterium liquid is prepared by following methods: yeast seeds is carried out slant strains cultivation on YPD solid medium, adds the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension in cultured slant strains; The bacteria suspension produced being cultivated on inclined-plane in YPD fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, saccharomycete bacterium liquid is obtained.
Step (2) described bacillus subtilis bacterium liquid is prepared by following methods: Bacillus subtilis strain is carried out slant strains cultivation on nutrient broth solid medium, in cultured slant strains, add the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension; The bacteria suspension produced being cultivated on inclined-plane in nutrient broth fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, bacillus subtilis bacterium liquid is obtained.
The present invention selects effect of raw material to be summarized as follows:
The Radix Astragali: there is the effects such as invigorating qi for strengthening superficies, sharp water detumescence, pus draining and toxin expelling, myogenic.
Radix Angelicae Sinensis: enrich blood; Invigorate blood circulation; Menstruction regulating and pain relieving; Moisturize laxation.
Poria cocos: the function having clearing damp and promoting diuresis, beneficial spleen and stomach, antitoxic heart-soothing and sedative.
The Chinese bulbul: clearing heat and detoxicating, removes heat from the blood and relieve diarrhea, antibacterial, cures mainly livestock and poultry toxic-heat and blood stasis, and damp and hot intestines are yellow, bacillary enteritis;
Bacillus acidi lactici, saccharomycete and bacillus subtilis: strengthen immunity of organisms, produce beneficial metabolic product and antibacterial material.
Chinese herbal medicine can replace antibiotic medicine, adds preventive dose in feed, can improve the gain in weight of weanling pig and reduce diarrhea rate; Probiotics by regulating animal gastrointestinal tract Micro Ecosystem balance, can reach prevent disease, promoting the active microorganism of growth of animal and raising efficiency of feed utilization.Through the Chinese herbal medicine that probiotics fermention is crossed, become Small molecular by large molecule, 100% to be absorbed by body the features such as, wide spectrum, green non-pollution, no drug residue efficient with it approve by numerous raisers.
The present invention obtains Chinese herbal microecological preparation by utilizing Bacillus acidi lactici, saccharomycete and bacillus subtilis to carry out fermentation to Chinese herbal medicine, have the following advantages: improve weanling pig immunity of organisms, prevent grice diarrhoea, promote digesting and assimilating of nutriment, reach the object of production performance of preventing and curing diseases, improve; Improve weanling pig weight; The medicament residue avoiding chemicals additive to cause and environmental pollution; Efficiently, have no side effect, noresidue.
Detailed description of the invention
Embodiment 1:
A kind of Chinese herbal microecological preparation improving weanling pig weight, be made up of the raw material of following weight portion: the Radix Astragali 6 ~ 15 parts, Radix Angelicae Sinensis 5 ~ 10 parts, 5 ~ 15 parts, Poria cocos, the Chinese bulbul 5 ~ 10 parts, Bacillus acidi lactici bacterium liquid 30 ~ 40 parts, saccharomycete bacterium liquid 30 ~ 40 parts and bacillus subtilis bacterium liquid 30 ~ 40 parts.
The preparation method of the Chinese herbal microecological preparation of this raising weanling pig weight, concrete steps are as follows:
(1) take the Radix Astragali, Radix Angelicae Sinensis, Poria cocos, the Chinese bulbul, pulverize after drying under 60 ~ 80 DEG C of conditions, cross 80 ~ 100 mesh sieves for subsequent use;
(2) extracting lactic acid bacillus bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid add in the herbal powder of step (1) gained respectively, employing closing anaerobic ferments, fermentation temperature is 30 DEG C ~ 37 DEG C, fermentation time is 36 ~ 72 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation.
Wherein, step (2) described Bacillus acidi lactici bacterium liquid is prepared by following methods: Lactobacillus species is carried out slant strains cultivation on MRS solid medium, adds the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension in cultured slant strains; The bacteria suspension produced being cultivated on inclined-plane in MRS fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, Bacillus acidi lactici bacterium liquid is obtained.
Step (2) described saccharomycete bacterium liquid is prepared by following methods: yeast seeds is carried out slant strains cultivation on YPD solid medium, adds the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension in cultured slant strains; The bacteria suspension produced being cultivated on inclined-plane in YPD fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, saccharomycete bacterium liquid is obtained.
Step (2) described bacillus subtilis bacterium liquid is prepared by following methods: Bacillus subtilis strain is carried out slant strains cultivation on nutrient broth solid medium, in cultured slant strains, add the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension; The bacteria suspension produced being cultivated on inclined-plane in nutrient broth fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, bacillus subtilis bacterium liquid is obtained.
Embodiment 2:
Accurately take the Radix Astragali 12 parts, Radix Angelicae Sinensis 8 parts, 12 parts, Poria cocos, the Chinese bulbul 8 parts, Bacillus acidi lactici bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 30 parts.
Preparation method is as follows:
(1) Radix Astragali 12 parts, Radix Angelicae Sinensis 8 parts, 12 parts, Poria cocos, the Chinese bulbul 8 parts is placed in 60 DEG C of oven dry in baking oven, and pulverize afterwards, 100 mesh sieves are for subsequent use excessively.
(2) difference extracting lactic acid bacillus bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 40 parts, add in the herbal powder of step (1) gained, fermentation temperature is 30 DEG C, fermentation time is 36 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation, can directly to feed piglet by spice.
The preparation method of step (2) described Bacillus acidi lactici bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid is with embodiment 1.
Embodiment 3:
Accurately take the Radix Astragali 15 parts, Radix Angelicae Sinensis 10 parts, 15 parts, Poria cocos, the Chinese bulbul 10 parts, Bacillus acidi lactici bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 30 parts.
Preparation method is as follows:
(1) Radix Astragali 15 parts, Radix Angelicae Sinensis 10 parts, 15 parts, Poria cocos, the Chinese bulbul 10 parts is placed in 70 DEG C of oven dry in baking oven, and pulverize afterwards, 80 mesh sieves are for subsequent use excessively.
(2) difference extracting lactic acid bacillus bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 30 parts, add in the herbal powder of step (1) gained, fermentation temperature is 37 DEG C, fermentation time is 48 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation, can directly to feed piglet by spice.
The preparation method of step (2) described Bacillus acidi lactici bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid is with embodiment 1.
Embodiment 4:
Accurately take the Radix Astragali 6 parts, Radix Angelicae Sinensis 5 parts, 5 parts, Poria cocos, the Chinese bulbul 5 parts, Bacillus acidi lactici bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 30 parts.
Preparation method is as follows:
(1) Radix Astragali 6 parts, Radix Angelicae Sinensis 5 parts, 5 parts, Poria cocos, the Chinese bulbul 5 parts is placed in 80 DEG C of oven dry in baking oven, and pulverize afterwards, 100 mesh sieves are for subsequent use excessively.
(2) difference extracting lactic acid bacillus bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 30 parts, add in the herbal powder of step (1) gained, fermentation temperature is 33 DEG C, fermentation time is 72 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation, can directly to feed piglet by spice.
The preparation method of step (2) described Bacillus acidi lactici bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid is with embodiment 1.
Embodiment 5:
Accurately take the Radix Astragali 10 parts, Radix Angelicae Sinensis 9 parts, 10 parts, Poria cocos, the Chinese bulbul 9 parts, Bacillus acidi lactici bacterium liquid 40 parts, saccharomycete bacterium liquid 35 parts and bacillus subtilis bacterium liquid 30 parts.
Preparation method is as follows:
(1) Radix Astragali 10 parts, Radix Angelicae Sinensis 9 parts, 10 parts, Poria cocos, the Chinese bulbul 9 parts is placed in 80 DEG C of oven dry in baking oven, and pulverize afterwards, 100 mesh sieves are for subsequent use excessively.
(2) difference extracting lactic acid bacillus bacterium liquid 40 parts, saccharomycete bacterium liquid 35 parts and bacillus subtilis bacterium liquid 30 parts, add in the herbal powder of step (1) gained, fermentation temperature is 34 DEG C, fermentation time is 60 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation, can directly to feed piglet by spice.
The preparation method of step (2) described Bacillus acidi lactici bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid is with embodiment 1.
Embodiment 6:
Accurately take the Radix Astragali 8 parts, Radix Angelicae Sinensis 7 parts, 8 parts, Poria cocos, the Chinese bulbul 7 parts, Bacillus acidi lactici bacterium liquid 35 parts, saccharomycete bacterium liquid 40 parts and bacillus subtilis bacterium liquid 35 parts.
Preparation method is as follows:
(1) Radix Astragali 8 parts, Radix Angelicae Sinensis 7 parts, 8 parts, Poria cocos, the Chinese bulbul 7 parts is placed in 60 DEG C of oven dry in baking oven, and pulverize afterwards, 100 mesh sieves are for subsequent use excessively.
(2) difference extracting lactic acid bacillus bacterium liquid 35 parts, saccharomycete bacterium liquid 40 parts and bacillus subtilis bacterium liquid 35 parts, add in the herbal powder of step (1) gained, fermentation temperature is 31 DEG C, fermentation time is 72 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation, can directly to feed piglet by spice.
The preparation method of step (2) described Bacillus acidi lactici bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid is with embodiment 1.
Embodiment 7:
Accurately take the Radix Astragali 9 parts, Radix Angelicae Sinensis 6 parts, 14 parts, Poria cocos, the Chinese bulbul 6 parts, Bacillus acidi lactici bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 40 parts.
Preparation method is as follows:
(1) Radix Astragali 9 parts, Radix Angelicae Sinensis 6 parts, 14 parts, Poria cocos, the Chinese bulbul 6 parts is placed in 60 DEG C of oven dry in baking oven, and pulverize afterwards, 100 mesh sieves are for subsequent use excessively.
(2) difference extracting lactic acid bacillus bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 40 parts, add in the herbal powder of step (1) gained, fermentation temperature is 35 DEG C, fermentation time is 40 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation, can directly to feed piglet by spice.
The preparation method of step (2) described Bacillus acidi lactici bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis bacterium liquid is with embodiment 1.
With concrete zoopery, effect of the present invention is described below:
Trial target: Chinese herbal microecological preparation of the present invention; Reference substance: normal diet
Experimental animal: each 30 of the weanling pig of 25 ages in days of Stochastic choice 4 plants.
Experimental design: the product of the plant one mixed feeding embodiment of the present invention 2, the product of the plant two mixed feeding embodiment of the present invention 3, the product of the plant three mixed feeding embodiment of the present invention 4, normal diet is fed by plant four.
Mixed feeding ratio: the addition 0.25% of Chinese herbal microecological preparation.
Test method: first three groups at regular time and quantity, feeds mixed feeding medicine, the 4th group in continuous 30 days, feeds pure feed in continuous 30 days; Observe the clinical change of piglet every day, line item of going forward side by side.
Clinical test results refers to following table:
As can be seen from above test data, the weightening finish index of plant one, two and three is all high than plant four, illustrate that the Chinese herbal and crude drugs preparations adding probio can improve the daily ingestion amount of weanling pig, improve daily gain and feedstuff-meat ratio, there is remarkable result to the weightening finish of weanling pig, prove that the present invention is feasible, reliable.
Above-described is only the preferred embodiment of the present invention; should be understood that; for a person skilled in the art; do not departing under general idea prerequisite of the present invention; some changes and improvements can also be made; these also should be considered as protection scope of the present invention, and these all can not affect effect of the invention process and practical applicability.
Claims (7)
1. one kind is improved the Chinese herbal microecological preparation of weanling pig weight, it is characterized in that: be made up of the raw material of following weight portion: the Radix Astragali 6 ~ 15 parts, Radix Angelicae Sinensis 5 ~ 10 parts, 5 ~ 15 parts, Poria cocos, the Chinese bulbul 5 ~ 10 parts, Bacillus acidi lactici bacterium liquid 30 ~ 40 parts, saccharomycete bacterium liquid 30 ~ 40 parts and bacillus subtilis bacterium liquid 30 ~ 40 parts.
2. the Chinese herbal microecological preparation of raising weanling pig weight according to claim 1, it is characterized in that: be made up of the raw material of following weight portion: the Radix Astragali 12 parts, Radix Angelicae Sinensis 8 parts, 12 parts, Poria cocos, the Chinese bulbul 8 parts, Bacillus acidi lactici bacterium liquid 30 parts, saccharomycete bacterium liquid 30 parts and bacillus subtilis bacterium liquid 40 parts.
3. a Chinese herbal microecological preparation preparation method for raising weanling pig weight according to claim 1, is characterized in that: concrete steps are as follows:
(1) take the Radix Astragali, Radix Angelicae Sinensis, Poria cocos, the Chinese bulbul, pulverize after drying, cross 80 ~ 100 mesh sieves for subsequent use;
(2) the bacterium liquid of extracting lactic acid bacillus bacterium liquid, saccharomycete bacterium liquid and bacillus subtilis adds in the herbal powder of step (1) gained respectively, employing closing anaerobic ferments, fermentation temperature is 30 DEG C ~ 37 DEG C, fermentation time is 36 ~ 72 hours, obtain tunning, through freeze drying, obtain Chinese herbal medicine compound micro-ecological preparation.
4. the Chinese herbal microecological preparation preparation method of raising weanling pig weight according to claim 3, is characterized in that: the bake out temperature in described step (1) is 60 ~ 80 DEG C.
5. the Chinese herbal microecological preparation preparation method of the raising weanling pig weight according to claim 3 or 4, it is characterized in that: step (2) described Bacillus acidi lactici bacterium liquid is prepared by following methods: Lactobacillus species is carried out slant strains cultivation on MRS solid medium, in cultured slant strains, add the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension; The bacteria suspension produced being cultivated on inclined-plane in MRS fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, Bacillus acidi lactici bacterium liquid is obtained.
6. the Chinese herbal microecological preparation preparation method of the raising weanling pig weight according to claim 3 or 4, it is characterized in that: step (2) described saccharomycete bacterium liquid is prepared by following methods: yeast seeds is carried out slant strains cultivation on YPD solid medium, in cultured slant strains, add the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension; The bacteria suspension produced being cultivated on inclined-plane in YPD fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, saccharomycete bacterium liquid is obtained.
7. the Chinese herbal microecological preparation preparation method of the raising weanling pig weight according to claim 3 or 4, it is characterized in that: step (2) described bacillus subtilis bacterium liquid is prepared by following methods: Bacillus subtilis strain is carried out slant strains cultivation on nutrient broth solid medium, in cultured slant strains, add the sterilized physiological saline of 1 ~ 3ml, prepare bacteria suspension; The bacteria suspension produced being cultivated on inclined-plane in nutrient broth fluid nutrient medium, expands cultivation, is 0.5 × 10 to exponential phase clump count
8cFU/ml ~ 1.0 × 10
8during CFU/ml only, bacillus subtilis bacterium liquid is obtained.
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| CN105707488A (en) * | 2016-02-29 | 2016-06-29 | 河南牧业经济学院 | Traditional Chinese medicine microecologics for enhancing piglet immunity and preparing method of traditional Chinese medicine microecologics |
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| CN109221697A (en) * | 2018-08-02 | 2019-01-18 | 广西遍天原种猪有限责任公司 | Weaned piglets probiotic feed and preparation method thereof |
| CN111494607A (en) * | 2020-04-26 | 2020-08-07 | 江苏德得健康产业有限公司 | Selenium-enriched post-growth-fermentation traditional Chinese medicine microbial preparation for improving growth performance of piglets and improving intestinal health and preparation method thereof |
| CN112190686A (en) * | 2020-11-22 | 2021-01-08 | 河北金麦维饲料科技有限公司 | Traditional Chinese medicine enzyme for replenishing qi and blood and improving immunity of livestock and poultry and preparation method thereof |
| CN114053383A (en) * | 2020-12-28 | 2022-02-18 | 焦作市农科利来生物科技有限公司 | Fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea and preparation method thereof |
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