CN102726605B - Micro-ecological synbiotic Chinese herbal medicine feed addictive, preparation method and application thereof - Google Patents
Micro-ecological synbiotic Chinese herbal medicine feed addictive, preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a micro-ecological synbiotic Chinese herbal medicine feed addictive, comprising a micro-ecological synbiotic feed addictive and a Chinese herbal medicine additive, wherein the micro-ecological synbiotic feed addictive comprises micro-ecological probiotics and oligosaccharide, the micro-ecological probiotics is a lactic acid bacillus preparation, and the oligosaccharide is one or more of fructo-oligo saccharide, xylo oligosaccharide, isomaltose oligosaccharide and soybean oligosaccharide, the weight of the micro-ecological synbiotic feed addictive accounts for 25-75 % of that of the total weight of the micro-ecological synbiotic Chinese herbal medicine feed addictive, and the Chinese herbal medicine additive comprises the following ingredients by weight parts: malt, pericarpium citri reticulatae viride, dried orange peel, rhizoma atractylodis, medicated leaven, coke hawthorn, coke gardenia, Chinese medical stone, folium artemisiae argyi, eucommia, dichroae radix, and panax japonicus. Separate application of the additive can raise animal culture production efficiency, reduce feeding cost, enhance the immunity and anti-stress capability of animals, and reduce incidence of diseases. Combined application of the additive and the Chinese herbal medicine additive has significant synergism.
Description
Technical field
The present invention relates to the livestock rearing field, particularly microbial forage additive.
Background technology
Along with the fast development of national economy and the raising day by day of living standards of the people, people have proposed higher, Secretary more to breeding environment and safety, healthy animal product.China is as the first in the world animal-breeding big country, the animal product outlet is very important in national economy, but some countries such as European, Japanese are take food-safety problem as barrier in recent years, and the part of the Chinese animal product of refusal enters, and cause unnecessary loss for China's economy.This a series of problem is all due to " animal production safety " problem, has become the bottleneck of the healthy and sustainable development of the Chinese aquaculture science of restriction.
China's ecologic breeding and regulation and control have in recent years obtained many progress, and generally believe at present in the world, and probiotics will have huge application prospect in the animal-breeding in future, will play larger effect to solving animal production safety.
Animal microecological formulation is according to the animal microecological theory, utilizes in animal body normal microorganism member and metabolite thereof or growth to promote thing, the preparation of making through special processing technology.Have additional, adjust or keep microbial balance in animal intestinal, reach diseases prevention, cure the disease, the purpose of promotion health and raising production performance.
Probio is the main body of probiotics, and probio is again active bacteria formulation, refers to bring into play beneficial effect for improving the animal microecological balance, reaches the raising animal level of the productive forces and the general level of the health and the artificial active bacteria formulation that adds.
At the beginning of last century (1907), the famous bacteriologist plum Cheney husband of section just proposes to drink the hypothesis that sour milk can be promoted longevity.Nineteen forty-seven, Meng Hade (Mollgaard) at first finds to use the Bacillus acidi lactici piglet of feeding, and can effectively increase the body weight of piglet, and improve the physical condition of piglet.Yet the 50-70 age in 20th century has caused the research and development of probiotics to be in low tide at antibiotic research just, the gold period of producing and using, and is not subject to enough attention, until the mid-80 has just had the change of essence to turn.
Japan develops in the world and utilizes one of probiotics country early, to the existing 26 kinds of products of the mid-80, it is reported, reaches existing more than 10 of enterprise more than 20,000,000,000 yen in Japanese probiotics annual value of production so far.
Other many countries, such as Germany, the U.S., France, Italy, Holland, Britain, Russia and Korea S etc. has dissimilar probiotics product, and some products have entered China market in recent years.As the Protexin of the PIC company of Britain, the Togocerin of Thailand's development and production adds in feed with 0.04% ratio, can improve in-pig and lactation period sow production performance, the diarrhea rate of piglet and the death rate are obviously descended.The Prosurs Paste (mainly containing bacillus, streptococcus fecalis, Bifidobacterium etc.) that Canada produces can make the growing and fattening pigs daily gain improve 9.5%.Use at present composite bacteria agent capables abroad more.As the Broilact of Finland, formed by 32 kinds of bacterium.The EM stoste of Japan belongs to more than 80 kind microorganisms by 5 sections 10 and is composited.
At present the main probiotics in domestic and international market is take Bacillus acidi lactici and bacillus subtilis as main, although the Bacillus acidi lactici acid producing ability is strong but thermo-labile, dead because of heating in the feed granulating process, so, additive for microbe feedstuff is selected the bacillus subtilis of the stronger high yield digestive ferment of heat resistance mostly, but bacillus subtilis lactic acid producing not.
Summary of the invention
The object of the present invention is to provide the mixture feed that forms with a kind of little ecological symphysis unit's feed addictive and Chinese herbal feed, the number of viable that described additive contains is high, can significantly promote the propagation of beneficial bacterium in young goose enteron aisle with the feed of this additive preparation, suppress the growth of harmful bacteria, the growth of livestock and poultry can be promoted again, the consumption of feed can also be reduced; Good stability, safely, have no side effect, noresidue, pollution-free.
For achieving the above object, technical scheme of the present invention is:
1, the first Chinese herbal feed additive of little ecological symphysis, formed by little ecological symphysis unit's feed addictive and Chinese herbal feed additive, the first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part, oligosaccharides 40-90 part, containing lactic acid bud pole bacterium viable count in the every gram of described little ecological symphysis unit's feed addictive is hundred million of 1-500, described little ecological probio is the lactic acid bacillus preparation, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides; The first feed addictive of described little ecological symphysis accounts for the 25-75% of the first Chinese herbal feed additive gross weight of described little ecological symphysis, and described Chinese herb feed additive is composed of the following components by weight: Fructus Hordei Germinatus 1-5 part, rascal 1-5 part, dried orange peel 1-5 part, rhizoma atractylodis 1-5 part, Divine Comedy 1-5 part, charred FRUCTUS CRATAEGI 1-5 part, burnt branch 1-5 part, medical stone 1-5 part, tarragon 1-5 part, bark of eucommia 1-5 part, Changshan 1-5 part, canton fairybells root and rhizome 0.1-1 part.
Further, in described little ecological symphysis unit feed addictive every gram to contain lactic acid bud pole bacterium viable count be hundred million of 10-300.
Further, the first feed addictive of described little ecological symphysis accounts for 45% of the first Chinese herbal feed additive gross weight of described little ecological symphysis, and described Chinese herb feed additive is composed of the following components by weight: 2 parts, Fructus Hordei Germinatus, 3 parts, rascal, 5 parts of dried orange peels, 4 parts of rhizoma atractylodis, 2 parts of Divine Comedy, 2 parts of charred FRUCTUS CRATAEGIs, 3 parts of burnt branches, 4 parts of medical stones, 3 parts of tarragons, 1 part of the bark of eucommia, 2 parts of Changshans, 0.5 part of canton fairybells root and rhizome.
The feed that contains described compositions of additives.
Further, containing mass fraction in described feed is 0.5% described little ecological symphysis unit Chinese herbal feed additive.
Another object of the present invention is to provide the preparation method of the first feed addictive of above-mentioned little ecological symphysis, the method is simple to operate, and production cost is low, is applicable to large-scale production.
Lactic acid bacillus being pressed the 4-6% inoculation of culture volume, is 6.0-8.0 in the pH value, and 37 ℃ of standing cultivations of constant temperature 24-72 hour get the lactic acid bacillus preparation; The lactic acid bacillus culture medium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, yeast extract 0.1-0.5 part, sodium chloride 0.1-0.5 part, MnSO
4.H
2O 0.001-0.01 part, CaCl
20.001-0.01 part, MgSO
4.7H
2O 0.001-0.1 part, aqueous medium 90-99 part, sterilization; The lactic acid bacillus preparation of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Described little ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, get the first Chinese herbal feed additive of described little ecological symphysis.
Wherein, described lactic acid bacillus preparation specifically comprises the following steps:
A enlarges kind of a daughter bacteria
Get on the inclined-plane bacterial classification and inoculate into the lactic acid bacillus culture medium being housed in vitro, 37 ℃ of standing cultivations of constant temperature 24 hours obtain the first order seed bacterium, take out Refrigerator store, and are stand-by;
The preparation of B secondary seed bacterium
With the ratio of first order seed bacterium in the 4-6% of lactic acid bacillus culture volume, be inoculated into sterilize and cooling lactic acid bacillus culture medium in, the 37 ℃ of standing cultivation of constant temperature 24h obtain the secondary seed bacterium; Take out Refrigerator store, stand-by; The lactic acid bacillus culture medium is poured in microorganism fermentation tank, and sterilization is also cooling;
The C fermented and cultured
Step B gained secondary seed bacterium in the also ratio inoculation of the 6-8% of cooling lactic acid bacillus kind culture volume of sterilizing, is stirred, and the 37 ℃ of standing cultivation of constant temperature 48-72h get nutrient solution;
The D centrifugal dehydration
In the centrifugal 20min of 3000r/min, remove supernatant after step C gained nutrient solution is cooling, get bacterium mud, add the filler that is equivalent to bacterium mud weight 1-2 times and mix, be placed under 40-50 ℃ of condition and dry, get the lactic acid bacillus preparation.
Wherein, the lactic acid bacillus culture medium prescription is preferably: 0.5 part of peptone, 0.3 part of beef extract, 0.1 part of yeast extract, 0.5 part, sodium chloride, MnSO
4.H
20.005 part of O, CaCl
20.00756 part, MgSO
4.7H
20.05 part of O, aqueous medium 90-99 part.
Beneficial effect of the present invention is: 1) compare with alone ecological symphysis unit feed addictive and have the following advantages: can improve animal-breeding production efficiency, reduce feeding cost; Can strengthen immunity and the anti-stress ability of animal, reduce the incidence of disease, the few or not medication of medication is conducive to health of human body and environmental protection; Be fit to modernization industry and produce, constant product quality; 2) Chinese herbal medicine in this formula, build spleen, the aid digestion master's of being compound Chinese medicinal preparation to regulate the flow of vital energy, reuse the reinforcings spleen to promote digestion such as Fructus Hordei Germinatus, rascal, dried orange peel, rhizoma atractylodis, Divine Comedy, charred FRUCTUS CRATAEGI, canton fairybells root and rhizome in the side, have the Chinese medicine of the merit of strong liver kidney concurrently, tarragon, medical stone, burnt branch, Changshan, the anti-diarrhoea of the bark of eucommia, increase trace element.Fructus Hordei Germinatus has digestion-aid effect because containing digestive ferment and Cobastab; Rascal has soothing the liver relieving stagnant Qi, the stagnant function of long-pendingization that disappear; Dried orange peel has to regulate the flow of vital energy builds spleen, eliminating dampness and eliminating phlegm effect; The rhizoma atractylodis function cures mainly drying damp and strengthening spleen, expelling wind and clearing away cold; Divine Comedy energy reinforcing spleen to promote digestion, regulating QI to expel dampness; But charred FRUCTUS CRATAEGI is food digesting stomach fortifying, the loose stasis of blood of promoting the circulation of qi; Burnt branch normalizing gallbladder to cure jaundice can promote pancreatic secretion; Medical stone can prevent diarrhoea and flatulence, improve the intestines function; Tarragon has the blood of regulating the flow of vital energy by the effect of cold-dampness, can prevent and treat trusted subordinate's crymodynia, the convulsion of having loose bowels, protracted dysentery tell nosebleed; The filling liver kidney of bark of eucommia tool, strengthening the bones and muscles, many effects such as hypotensive; Changshan is vomiting phlegmatic savliva, preventing malaria; Canton fairybells root and rhizome can be clearing heat and detoxicating, relaxes the muscles and stimulate the blood circulation.
The specific embodiment
The preparation of the first feed addictive of the little ecological symphysis of embodiment 1
One, taking oligosaccharides is that each 10Kg of FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides is standby;
Two, the preparation of lactic acid bacillus preparation:
Bacterial classification is the lactic acid bacillus (lactic acid bacillus) that freeze-drying is preserved; My listed as parts by weight of lactic acid bacillus culture medium is composed of the following components: 0.5 part of peptone, 0.3 part of beef extract, 0.1 part of yeast extract, 0.5 part, sodium chloride, MnSO
4Part .H
20.005 part of O, CaCl
20.00756 part, MgSO
4.7H
20.05 part of O, 99 parts of ultra-pure waters, 6.8,115 ℃ of high pressure steam sterilization 30min of pH;
1. enlarge kind of a daughter bacteria, get bacterial classification on the inclined-plane (as an oese) inoculation and enter to be equipped with the lactic acid bacillus culture medium in vitro, the 37 ℃ of standing cultivation of constant temperature 24h obtain the first order seed bacterium, take out Refrigerator store, and are stand-by;
2. the preparation of secondary seed bacterium: with 5% the ratio of first order seed bacterium in the volume of lactic acid bacillus culture medium, be inoculated in the lactic acid bacillus culture medium, the 37 ℃ of standing cultivation of constant temperature 24h obtain the secondary seed bacterium;
3. produce the preparation of the lactic acid bacteria culture medium of use: take each composition in the composition ratio of lactic acid bacillus culture medium and be dissolved in water, pour in microorganism fermentation tank, in 115 ℃ of steam sterilizations 30 minutes;
4. inoculation: the lactic acid bacillus kind culture medium of use to be produced is chilled to 37 ℃ of left and right, adds the secondary seed bacterium under flame is controlled, and inoculates by the ratio of the 6-8% of lactic acid bacillus kind culture volume, stirs;
5. cultivate: close all breather valves, 37 ℃ of constant temperature culture 60h;
6. centrifugal dehydration: take out nutrient solution in biological fermentation tank, refrigerator is temporary, until nutrient solution cooling after in 3000 rev/mins centrifugal 20 minutes, remove supernatant, get bacterium mud and add 2 times of chaff powder to mix, the disk of packing into, be placed in oven dry in 45 ℃ of air dry ovens, carry out the total count plate of lactic acid bacteria by GB 4789.35-2010 method and detect, pack, get the lactic acid bacillus preparation, keep in Dark Place standby at shady and cool dry place.
The preparation of the three first feed addictives of ecological symphysis
1) ecological symphysis unit's feed addictive prescription one (being called for short additive 1): calculate by weight composed of the following components: 40 parts, lactic acid bacillus preparation, 90 parts of FOSs, wherein, in the first feed addictive of the ecological symphysis of every gram, viable count is 30,000,000,000.
The first Chinese herbal feed additive formula one of little ecological symphysis (being called for short composition 1): the first feed addictive prescription one of ecological symphysis accounts for 30% of compositions of additives gross weight, and surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 1), and described Chinese herb feed additive is composed of the following components by weight: 1 part, Fructus Hordei Germinatus, 1 part, rascal, 1 part of dried orange peel, 1 part of rhizoma atractylodis, 1 part of Divine Comedy, 5 parts of charred FRUCTUS CRATAEGIs, 5 parts of burnt branches, 5 parts of medical stones, 5 parts of tarragons, 5 parts of the barks of eucommia, 5 parts of Changshans, 0.5 part of canton fairybells root and rhizome.
2) ecological symphysis unit's feed addictive prescription two (being called for short additive 2): calculate by weight composed of the following components: 70 parts, lactic acid bacillus preparation, 30 parts of xylo-oligosaccharides, wherein, in the first feed addictive of the ecological symphysis of the every gram of every gram, viable count is 20,000,000,000.
The first Chinese herbal feed additive formula two of little ecological symphysis (being called for short compositions 2): the first feed addictive prescription one of ecological symphysis accounts for 75% of compositions of additives gross weight, and surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 2), and described Chinese herb feed additive is composed of the following components by weight: 5 parts, Fructus Hordei Germinatus, 5 parts, rascal, 5 parts of dried orange peels, 5 parts of rhizoma atractylodis, 5 parts of Divine Comedy, 1 part of charred FRUCTUS CRATAEGI, 1 part of burnt branch, 1 part of medical stone, 1 part of tarragon, 1 part of the bark of eucommia, 1 part of Changshan, 0.5 part of canton fairybells root and rhizome.
3) ecological symphysis unit's feed addictive prescription three (being called for short additive 3): calculate by weight composed of the following components: 40 parts, lactic acid bacillus preparation, 60 parts of isomalto-oligosaccharides, wherein, in the first feed addictive of the ecological symphysis of every gram, viable count is 30,000,000,000.
The first Chinese herbal feed additive formula three of little ecological symphysis (being called for short compositions 3): the first feed addictive prescription one of ecological symphysis accounts for 45% of compositions of additives gross weight, and surplus is Chinese herb feed additive (being called for short Chinese herbal medicine 3), and described Chinese herb feed additive is composed of the following components by weight: 2 parts, Fructus Hordei Germinatus, 3 parts, rascal, 5 parts of dried orange peels, 4 parts of rhizoma atractylodis, 2 parts of Divine Comedy, 2 parts of charred FRUCTUS CRATAEGIs, 3 parts of burnt branches, 4 parts of medical stones, 3 parts of tarragons, 1 part of the bark of eucommia, 2 parts of Changshans, 0.5 part of canton fairybells root and rhizome.The mouthfeel of this formula is best.
Each component in the said herbal medicine additive, see " national Chinese herbal medicine compilation " (Xie Zongwan chief editor for details, the People's Health Publisher, 1996-1052) and " Chinese medicine voluminous dictionary " (Nanjing University of Traditional Chinese Medicine writes, Shanghai science tech publishing house publication time: the 2006-3-1 second edition).
Each component of formula ratio is mixed in proportion, and the granulation agent is so that quality control, preservation, transportation and use.
The feeding effect of embodiment 2 ducklings
The test newly-hatched duckling is selected Cherry Village Ducks, testing the same age in days of initial selection goes out strong young 240 of shell and concentrates the week of brooding to be divided at random 4 groups every group 60 after age, if 4 repetitions, 15 of every repetitions, claim individual weight, after reaching homogeneity P＞0.05 between each group of Analysis of variance, these 4 groups examination ducks are defined as 4 kinds of different disposal group feeding experiment duck material, 44 days experimental periods at random.Each is organized Diet combination and sees Table 1.
Table 1 experiment grouping and Diet combination
Described basestocks is the conventional young goose feed of market sale, and above-mentioned percentage is weight percentage
The impact (g) on the duckling production performance of table 2 various combination and consumption
As can be seen from Table 2, in the 1-14 age in days stage, daily gain, feed intake and feedstuff-meat ratio are tested 4 groups and extremely significantly are better than control group and other test group.
The impact on Escherichia coli, lactic acid bacteria and Bifidobacterium of table 3 probiotic and little ecological symphysis tuple
As can be seen from Table 3, the coliform count in group 2 and group 4 rectum ight soil is lower than control group, and Bifidobacterium, Bacillus acidi lactici are counted test group all higher than control group, and is especially remarkable with group 4.Show and throw something and feed probiotic especially after probiotic and prebiotics are used in conjunction with, thereby the propagation that can obviously promote the beneficial bacteriums such as Bifidobacterium and Bacillus acidi lactici improves the quantity of beneficial bacterium in enteron aisle, and the harmful bacteria Escherichia coli is suppressed and quantity obviously reduces.The increase of beneficial bacteria of intestinal tract quantity can activate enteron aisle digestion and immunologic function, promotes digestion and the absorption of young goose, and suppresses the breeding of harmful bacteria, reduces the generation of intestines problem, thereby plays the short long effect of health care.The present invention compares with the first feed addictive of alone ecological symphysis and has the following advantages: can improve young goose breeding production efficient, reduce feeding cost; Can strengthen immunity and the anti-stress ability of young goose, reduce the incidence of disease, the few or not medication of medication is conducive to health of human body and environmental protection; Be fit to modernization industry and produce, constant product quality.
The feeding effect of embodiment 3 piglets
Selection grow normal same parity, length that body weight is more consistent * large binary ablactation piglet.To be divided at random 5 groups by body weight, sex for the examination pig, every group of 21 piglets are established 3 repetitions in group, and each repeats 7 piglets, and are interior to organize, be principle without significant difference (P＞0.05) between organizing.Experimental period is 30d, preliminary trial period 2d.Each is organized Diet combination and sees Table 4.
Table 4 experiment grouping and Diet combination
Described basestocks is the conventional pig starter feed of market sale; Above-mentioned percentage is weight percentage
The impact (g) on weaned piglets of table 5 various combination and consumption
Annotate: the data average ± standard deviation; Same line number shoulder mark
*With
*Be illustrated on 0.05 and 0.01 level difference remarkable.
As can be seen from Table 5,8 groups of piglet daily gain, feed intake and feedstuff-meat ratio tests extremely significantly be better than control group and other test group (
*P＜0.05;
*P＜0.01)
The impact on escherichia coli of piglets, lactic acid bacteria and Bifidobacterium of table 6 probiotic and Synbiotics group
As can be seen from Table 6, the coliform count in group 5 and group 8 rectum ight soil is lower than control group, and Bifidobacterium, Bacillus acidi lactici are counted test group all higher than control group, and is especially remarkable with group 8.Show and throw something and feed probiotic especially after probiotic and prebiotics are used in conjunction with, thereby the propagation that can obviously promote the beneficial bacteriums such as Bifidobacterium and Bacillus acidi lactici improves the quantity of beneficial bacterium in enteron aisle, and the harmful bacteria Escherichia coli is suppressed and quantity obviously reduces.The increase of beneficial bacteria of intestinal tract quantity can activate enteron aisle digestion and immunologic function, promotes digestion and the absorption of young pig, and suppresses the breeding of harmful bacteria, reduces the generation of intestines problem, thereby plays the short long effect of health care.The present invention compares with the first feed addictive of alone ecological symphysis and has the following advantages: can improve young pig breeding production efficient, reduce feeding cost; Can strengthen immunity and the anti-stress ability of young pig, reduce the incidence of disease, the few or not medication of medication is conducive to health of human body and environmental protection; Be fit to modernization industry and produce, constant product quality.
In addition, after long-term feeding, the discovery animal is felt the preference degree of group 4 and group 8 most, and each component of described Chinese herb feed additive and weight portion are mutually harmonious, and the mouthfeel of its allotment is best.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment, the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not breaking away from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (7)
1. little ecological symphysis unit Chinese herbal feed additive, it is characterized in that, formed by little ecological symphysis unit's feed addictive and Chinese herb feed additive, the first feed addictive of little ecological symphysis is composed of the following components according to listed as parts by weight: little ecological probio 10-60 part, oligosaccharides 40-90 part, containing viable count of lactic acid bacillus in the every gram of described little ecological symphysis unit's feed addictive is hundred million of 1-500, described little ecological probio is the lactic acid bacillus preparation, and described oligosaccharides is any one or more in FOS, xylo-oligosaccharide, isomalto-oligosaccharide and soyabean oligosaccharides; The first feed addictive of described little ecological symphysis accounts for the 25-75% of the first Chinese herbal feed additive gross weight of described little ecological symphysis, and described Chinese herb feed additive is composed of the following components by weight: Fructus Hordei Germinatus 1-5 part, rascal 1-5 part, dried orange peel 1-5 part, rhizoma atractylodis 1-5 part, Divine Comedy 1-5 part, charred FRUCTUS CRATAEGI 1-5 part, burnt branch 1-5 part, medical stone 1-5 part, tarragon 1-5 part, bark of eucommia 1-5 part, Changshan 1-5 part, canton fairybells root and rhizome 0.1-1 part.
2. little ecological symphysis according to claim 1 unit Chinese herbal feed additive, is characterized in that, in described little ecological symphysis unit feed addictive, to contain viable count of lactic acid bacillus be hundred million of 10-300 to every gram.
3. little ecological symphysis according to claim 1 unit Chinese herbal feed additive is characterized in that: described little ecological symphysis unit feed addictive accounts for 45% of described little ecological symphysis unit Chinese herbal feed additive gross weight, and described Chinese herb feed additive is composed of the following components by weight: 2 parts, Fructus Hordei Germinatus, 3 parts, rascal, 5 parts of dried orange peels, 4 parts of rhizoma atractylodis, 2 parts of Divine Comedy, 2 parts of charred FRUCTUS CRATAEGIs, 3 parts of burnt branches, 4 parts of medical stones, 3 parts of tarragons, 1 part of the bark of eucommia, 2 parts of Changshans, 0.5 part of canton fairybells root and rhizome.
4. the preparation method of the described little ecological symphysis of claim 1-3 any one unit Chinese herbal feed additive, it is characterized in that, lactic acid bacillus is pressed the 4-6% inoculation of culture volume, is 6.0-8.0 in the pH value, 37 ℃ of standing cultivations of constant temperature 24-72 hour get the lactic acid bacillus preparation; The lactic acid bacillus culture medium calculates composed of the following components by weight: peptone 0.1-1.0 part, beef extract 0.1-0.5 part, yeast extract 0.1-0.5 part, sodium chloride 0.1-0.5 part, MnSO
4H
2O 0.001-0.01 part, CaCl
20.001-0.01 part, MgSO
47H
2O 0.001-0.1 part, aqueous medium 90-99 part, sterilization; The lactic acid bacillus preparation of formula ratio and the FOS of formula ratio are fully mixed, get the first feed addictive of little ecological symphysis; Described little ecological symphysis unit's feed addictive of formula ratio and each component of described Chinese herb feed additive are fully mixed, get the first Chinese herbal feed additive of described little ecological symphysis.
5. preparation method according to claim 4, is characterized in that the lactic acid bacillus culture medium prescription: 0.5 part of peptone, 0.3 part of beef extract, 0.1 part of yeast extract, 0.5 part, sodium chloride, MnSO
4H
20.005 part of O, CaCl
20.00756 part, MgSO
47H
20.05 part of O, aqueous medium 90-99 part.
6. the feed that contains the first Chinese herbal feed additive of little ecological symphysis claimed in claim 1.
7. feed according to claim 6, is characterized in that, contains mass fraction in described feed and be 0.5% described little ecological symphysis unit Chinese herbal feed additive.
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| CN103191180B (en) * | 2013-04-12 | 2015-03-18 | 重庆市畜牧科学院 | Preparation for preventing and treating chickling salmonella enterica white diarrhea and preparation method |
| CN104522403A (en) * | 2014-12-26 | 2015-04-22 | 太仓安佑生物科技有限公司 | Special synbiotics for piglet creep feed |
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