CN104350954B - Cornu Cervi Pantotrichum mushroom cultivation method - Google Patents

Cornu Cervi Pantotrichum mushroom cultivation method Download PDF

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CN104350954B
CN104350954B CN201410700135.9A CN201410700135A CN104350954B CN 104350954 B CN104350954 B CN 104350954B CN 201410700135 A CN201410700135 A CN 201410700135A CN 104350954 B CN104350954 B CN 104350954B
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parts
days
illumination
bottle
mycelium stimulation
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CN104350954A (en
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贲伟东
杨仁智
向银春
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JIANGSU GUBENTANG BIOLOGICAL TECHNOLOGY Co Ltd
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JIANGSU GUBENTANG BIOLOGICAL TECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/40Fertilisers incorporated into a matrix

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The present invention relates to a kind of Cornu Cervi Pantotrichum mushroom cultivation method, weigh culture medium raw material by mass fraction;Raw material mixes, and controls pH value 67, loads culture bottle, and 400 500 parts every bottle, gland encapsulates;Culture bottle sends into vacreation pot, and ozone sterilization is suction 50 80KPa after 12 hours, sterilizes 100 120 minutes at 150 DEG C;Cool down at 15 20 DEG C, add humic acid 14 parts, after cooling, send into every bottle graft original seed 40 50;20 25 DEG C of culturing room, humidity 70 80%, between gas concentration lwevel 1600 3200PPM, cultivate 20 25 days;Mycelium stimulation processes, and utilizes mushroom culturing device to carry out mycelium stimulation process and carries out moisturizing by the 85% of old mycoderma weight simultaneously;Flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 14 15 DEG C, 3 days intensity of illumination 0 50Lux in humidity 95 98%, below gas concentration lwevel 1200PPM, 1,46 days intensity of illumination 50 100Lux, 7 10 days intensity of illumination 150 200Lux, 10 20 days intensity of illumination 200 300Lux, started to gather after 21 days.

Description

Cornu Cervi Pantotrichum mushroom cultivation method
Technical field
The present invention relates to a kind of Cornu Cervi Pantotrichum mushroom cultivation method.
Background technology
Cornu Cervi Pantotrichum mushroom also known as Lyophyllum decastes (Lyophyllum decastes (Fr.: Fr.) Sing.), Folium Nelumbinis mushroom, The Yunnan of China is also called cold fragrant bacterium, a brood of sheep, Fungus Pleurotus ostreatus etc., belongs to Basidiomycotina, and Hymenomycetes, cap mesh, Bai Mo section, from umbrella Pseudomonas, is referred to as fried chicken mushroom in Europe, and bacterial context is plump, fine and smooth, A sweety scent assails the nostrils, delicious flavour, and research shows in Cornu Cervi Pantotrichum massee fruiting bodies Crude protein, amino acid whose content are higher, and fat content is relatively low;But also containing trace element zinc, copper and the selenium useful to human body And substantial amounts of vitamin B1, B2, B6, B12 and nicotinic acid, there is the highest nutritive value.Show according to modern scientific research, Cornu Cervi Pantotrichum mushroom The beta glucan contained, has been eaten for a long time antineoplastic effect, and meanwhile, Cornu Cervi Pantotrichum mushroom has blood pressure lowering, reduces cholesterol, anti-glycosuria The pharmacologic actions such as disease, antiallergic, energy strengthening by means of tonics, strengthening the body resistance, strengthen immunity, slow down aging.
The most domestic research for the cultivation of Cornu Cervi Pantotrichum mushroom has focused largely in the research of biological character, to cultivation research relatively Few, within 2007, industrialized cultivation Cornu Cervi Pantotrichum mushroom is reported by Shanghai Finc Bio-tech Inc. first.This article is same Time point out to guarantee that the low stain rate that factory culture brings benefits is that cultivation is the most crucial.The link of Environmental capacity most critical is Strain can realize quickly surely growing on compost, and the vigor of strain is then quickly surely to grow most important factor.The Inner Mongol in 2008 Autonomous region Yakeshi City Yi Tuli river forestry bureau feels that Lyophyllum decastes (Cornu Cervi Pantotrichum mushroom) cultural method patent has been applied in careless Xun forest farm, adopts Using solid spawn planting type, solid spawn preparation cost is long, and inoculation efficiency is low, and strain germination physiology is slow, the pollution of culture bottle Rate is high.
Summary of the invention
The invention provides a kind of preparation cost low, inoculation efficiency, strain germination physiology, reduces the deer of culture bottle pollution rate Fine and soft mushroom liquid strain preparation method.
The technical solution used in the present invention is: a kind of Cornu Cervi Pantotrichum mushroom cultivation method, it is characterised in that:
(1), culture medium raw material is weighed by mass fraction: wood flour 40-60 part, Testa oryzae 10-20 part, wheat bran 5-10 part, continuous seed Shell 10-20 part, Calx 1-3 part, Nutrition Soil 10-15 part, water 80-110 part;
(2), being mixed by step (1) raw material, control pH value 6-7, load culture bottle, every bottle of 400-500 part, gland encapsulates;
(3), culture bottle send into vacreation pot, ozone sterilization is suction 50-80KPa after 1-2 hour, kills at 150 DEG C Bacterium 100-120 minute;
(4), cool down at 15-20 DEG C, add humic acid 1-4 part, after cooling, send into every bottle graft original seed 40-50;
(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivate 20-25 My god;
(6), mycelium stimulation process, utilize mushroom culturing device carry out mycelium stimulation process carry out moisturizing by the 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to process, in culturing room after mycelium stimulation, temperature 14-15 DEG C, humidity 95-98%, gas concentration lwevel Below 1200PPM, 1-3 days intensities of illumination 0-50Lux, 4-6 days intensities of illumination 50-100Lux, 7-10 days intensities of illumination 150- 200Lux, 10-20 days intensities of illumination 200-300Lux, started to gather after 21 days.
Compared with prior art, the invention have the advantage that culture medium is easy to make, low cost, and for liquid spawn system When making, it is fast that strain sends out bacterium speed, pollution-free, without old mycoderma, is conducive to improving and sends out bacterium effect and yield.Add humic acid, effectively Improve rate of buddingging, it is ensured that mushroom yield.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment one
A kind of Cornu Cervi Pantotrichum mushroom liquid strain preparation method:
(1) culture medium raw material, by mass fraction is weighed: wood flour 40 parts, 10 parts of Testa oryzae, 5 parts of wheat bran, continuous 10 parts of seed shell, stone Ash 1 part, Nutrition Soil 10 parts, 80 parts of water;
(2), being mixed by step (1) raw material, control pH value 6, load culture bottle, 400 parts every bottle, gland encapsulates;
(3), culture bottle send into vacreation pot, ozone sterilization is suction 50KPa after 1 hour, at 150 DEG C sterilize 100 Minute;
(4), cool down at 15 DEG C, add humic acid 1 part, after cooling, send into every bottle graft original seed 40;
(5), 20 DEG C of culturing room, humidity 70%, between gas concentration lwevel 1600PPM, cultivate 20 days;
(6), mycelium stimulation process, utilize mushroom culturing device carry out mycelium stimulation process carry out moisturizing by the 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 14 DEG C, humidity 95%, below gas concentration lwevel 1200PPM, 1-3 days intensities of illumination 0-50Lux, 4-6 days intensities of illumination 50-100Lux, 7-10 days intensities of illumination 150-200Lux, 10-20 days Intensity of illumination 200-300Lux, started to gather after 21 days.
Embodiment two
A kind of Cornu Cervi Pantotrichum mushroom liquid strain preparation method:
(1) culture medium raw material, by mass fraction is weighed: wood flour 60 parts, 20 parts of Testa oryzae, 10 parts of wheat bran, continuous 20 parts of seed shell, stone Ash 3 parts, Nutrition Soil 15 parts, 110 parts of water;
(2), being mixed by step (1) raw material, control pH value 7, load culture bottle, 500 parts every bottle, gland encapsulates;
(3), culture bottle send into vacreation pot, ozone sterilization is suction 80KPa after 2 hours, at 150 DEG C sterilize 120 Minute;
(4), cool down at 20 DEG C, add humic acid 2 parts, after cooling, send into every bottle graft original seed 50;
(5), 25 DEG C of culturing room, humidity 80%, between gas concentration lwevel 3200PPM, cultivate 25 days;
(6), mycelium stimulation process, utilize mushroom culturing device carry out mycelium stimulation process carry out moisturizing by the 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 15 DEG C, humidity 98%, below gas concentration lwevel 1200PPM, 1-3 days intensities of illumination 0-50Lux, 4-6 days intensities of illumination 50-100Lux, 7-10 days intensities of illumination 150-200Lux, 10-20 days Intensity of illumination 200-300Lux, started to gather after 21 days.
Embodiment three
A kind of Cornu Cervi Pantotrichum mushroom liquid strain preparation method:
(1) culture medium raw material, by mass fraction is weighed: wood flour 50 parts, 15 parts of Testa oryzae, 8 parts of wheat bran, continuous 15 parts of seed shell, stone Ash 2 parts, Nutrition Soil 13 parts, 90 parts of water;
(2), being mixed by step (1) raw material, control pH value 6, load culture bottle, 450 parts every bottle, gland encapsulates;
(3), culture bottle send into vacreation pot, ozone sterilization is suction 60KPa after 1.5 hours, at 150 DEG C sterilize 110 minutes;
(4), cool down at 18 DEG C, add humic acid 4 parts, after cooling, send into every bottle graft original seed 46;
(5), 22 DEG C of culturing room, humidity 78%, between gas concentration lwevel 2200PPM, cultivate 22 days;
(6), mycelium stimulation process, utilize mushroom culturing device carry out mycelium stimulation process carry out moisturizing by the 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 14 DEG C, humidity 98%, below gas concentration lwevel 1200PPM, 1-3 days intensities of illumination 0-50Lux, 4-6 days intensities of illumination 50-100Lux, 7-10 days intensities of illumination 150-200Lux, 10-20 days Intensity of illumination 200-300Lux, started to gather after 21 days.

Claims (1)

1. a Cornu Cervi Pantotrichum mushroom cultivation method, it is characterised in that:
(1) culture medium raw material, by mass fraction is weighed: wood flour 50 parts, 15 parts of Testa oryzae, 8 parts of wheat bran, continuous 15 parts of seed shell, Calx 2 Part, Nutrition Soil 13 parts, 90 parts of water;
(2), being mixed by step (1) raw material, control pH value 6, load culture bottle, 450 parts every bottle, gland encapsulates;
(3), culture bottle send into vacreation pot, ozone sterilization is suction 60KPa after 1.5 hours, at 150 DEG C sterilize 110 points Clock;
(4), cool down at 18 DEG C, add humic acids 4 parts, after cooling, send into every bottle graft original seed 46;
(5), 22 DEG C of culturing room, humidity 78%, gas concentration lwevel 2200PPM, cultivates 22 days;
(6), mycelium stimulation process, utilize mushroom culturing device carry out mycelium stimulation process carry out moisturizing by the 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 14 DEG C, humidity 98%, below gas concentration lwevel 1200PPM, 1-3 It intensity of illumination 0-50Lux, 4-6 days intensities of illumination 50-100Lux, 7-10 days intensities of illumination 150-200Lux, illumination in 10-20 days Intensity 200-300Lux, started to gather after 21 days.
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Publication number Priority date Publication date Assignee Title
CN105218197B (en) * 2015-11-02 2018-07-13 江苏农牧科技职业学院 A kind of pilose antler mushroom cultivation medium formula and preparation method thereof
CN109548561A (en) * 2018-12-25 2019-04-02 昆山青禾食用菌科技有限公司 A kind of pilose antler mushroom culture method
CN110663453B (en) * 2019-11-15 2022-08-26 昆山青禾食用菌科技有限公司 Lyophyllum decastes culture material, preparation method and application
CN111296173A (en) * 2020-03-27 2020-06-19 江苏华绿生物科技股份有限公司 Industrialized cultivation medium for velvet antler mushroom and method for cultivating edible fungi
CN111512885A (en) * 2020-04-28 2020-08-11 江苏华绿生物科技股份有限公司 Preparation process for industrially cultivating velvet antler mushroom
CN111771616A (en) * 2020-08-18 2020-10-16 湖南和平生物科技有限公司 Industrialized cultivation method of velvet antler mushroom
CN113412760A (en) * 2021-06-22 2021-09-21 江苏华绿生物科技股份有限公司 Hypsizigus marmoreus culture medium and hypsizigus marmoreus cultivation process
CN113796263A (en) * 2021-09-13 2021-12-17 贵州大秦农业科技有限公司 Large-scale cultivation method of velvet antler mushroom
CN116171797B (en) * 2023-04-04 2024-06-07 福建恒绿生物科技有限公司 Cultivation method of velvet mushrooms

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JP3542945B2 (en) * 1990-03-08 2004-07-14 タカラバイオ株式会社 Artificial cultivation method of Hatake Shimeji
JP4925169B2 (en) * 2005-07-26 2012-04-25 ヤマサ醤油株式会社 Artificial cultivation method and medium of Honshimeji
CN103210849B (en) * 2013-03-04 2014-07-09 上海丰科生物科技股份有限公司 Lyophyllum decastes new bacterial strain
CN103340156B (en) * 2013-03-25 2014-11-19 上海丰科生物科技股份有限公司 Novel strain of lyophyllum decastes

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