CN104450539A - Preparation method of velvet antler mushroom liquid strain - Google Patents
Preparation method of velvet antler mushroom liquid strain Download PDFInfo
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- CN104450539A CN104450539A CN201410700133.XA CN201410700133A CN104450539A CN 104450539 A CN104450539 A CN 104450539A CN 201410700133 A CN201410700133 A CN 201410700133A CN 104450539 A CN104450539 A CN 104450539A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention relates to a preparation method of a velvet antler mushroom liquid strain. The velvet antler mushroom liquid strain comprises the following components in parts by weight: 10-30 parts of cane sugar, 2-5 parts of bean pulp, 1-3 parts of a beef extract, 0.3-1 part of magnesium sulfate heptahydrate, 0.3-1 parts of potassium dihydrogen phosphate and 0.1-0.3 part of a de-foaming agent. The preparation method has the advantages that a culture medium is convenient in operation and low in cost; when the culture medium is used for liquid strain production, the strain growing speed is high, no pollution and no old mycoderma are generated, and the strain growing effect and the yield are favorably improved.
Description
Technical field
The present invention relates to a kind of pilose antler mushroom liquid nutrient medium and preparation method, liquid spawn preparation method.
Background technology
Pilose antler mushroom is also known as lyophyllum decaste (Lyophyllum decastes (Fr.: Fr.) Sing.), and lotus leaf mushroom, is also called cold fragrant bacterium in the Yunnan of China, a brood of sheep, Fungus Pleurotus ostreatus etc., belong to Basidiomycotina, Hymenomycetes, cap order, Bai Mo section, from Agaricus, be called fried chicken mushroom in Europe, bacterial context is plump, fine and smooth, A sweety scent assails the nostrils, delicious flavour, research shows that crude protein in pilose antler massee fruiting bodies, amino acid whose content are higher, and lipid content is lower; But also contain useful trace element zinc, copper and the selenium of human body and a large amount of VITMAIN B1, B2, B6, B12 and nicotinic acid, there is very high nutritive value.Show according to modern scientific research, the beta glucan that pilose antler mushroom contains, long-term eating has antineoplastic effect, meanwhile, pilose antler mushroom have hypotensive, reduce the pharmacologic action such as cholesterol, anti-diabetic, antianaphylaxis, can strengthening by means of tonics, strengthen the body resistance to consolidate the constitution, strengthen immunity, delay senility.
The domestic research for the cultivation of pilose antler mushroom at present concentrates in the research of biological character mostly, and less to cultivation research, within 2007, Shanghai Finc Bio-tech Inc. reports industrialized cultivation pilose antler mushroom first.This article is pointed out to guarantee that the low stain rate that factory culture brings benefits is that cultivation is successfully crucial simultaneously.The link of Environmental capacity most critical is that bacterial classification can realize surely growing fast on culture material, and the vigor of bacterial classification is then surely grow most important factor fast.Inner Mongolia Autonomous Region in 2008 Yakeshi City Yi Tuli river forestry bureau feels that lyophyllum decaste (pilose antler mushroom) cultivating method patent has been applied in careless Xun forest farm, adopt solid spawn planting type, solid spawn preparation cost is long, and inoculation efficiency is low, bacterial classification germination physiology is slow, and the pollution rate of culturing bottle is high.
Summary of the invention
The invention provides a kind of preparation cost low, inoculation efficiency, bacterial classification germination physiology, reduce the preparation method of the pilose antler mushroom liquid nutrient medium of culturing bottle pollution rate and substratum thereof, bacterial classification.
The technical solution used in the present invention is: a kind of pilose antler mushroom liquid nutrient medium, is characterized in that: comprise sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part by mass fraction.
Further, sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part is comprised by mass fraction.
A preparation method for pilose antler mushroom liquid nutrient medium, is characterized in that, comprise the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part;
(2), by step (1) raw material mix, control pH value 5.5-6.5, load culturing bottle, every bottle of 400-500 part, gland encapsulates;
(3), culturing bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, insulation 30 minutes at 115 DEG C, insulation 60 minutes at 121 DEG C.
A preparation method for pilose antler mushroom liquid strain, is characterized in that, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part;
(2), by step (1) raw material mix, control pH value 5.5-6.5, load culturing bottle, every bottle of 400-500 part, gland encapsulates;
(3), culturing bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, insulation 30 minutes at 115 DEG C, insulation 60 minutes at 121 DEG C.
(4), at 15-20 DEG C cool, after cooling, send into every bottle graft original seed 40-50;
(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivates 20-25 days.
Compared with prior art, advantage of the present invention is: substratum is easy to make, cost is low, and when making for liquid spawn, it is fast that bacterial classification sends out bacterium speed, pollution-free, without old mycoderma, is conducive to improving and sends out bacterium effect and output.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment one
A kind of pilose antler mushroom liquid nutrient medium, comprises sucrose 10 parts, dregs of beans 2 parts, extractum carnis 1 part, magnesium sulfate heptahydrate 0.3 part, potassium primary phosphate 0.3 part, defoamer 0.1 part by mass fraction.
The preparation method of pilose antler mushroom liquid nutrient medium, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10 parts, dregs of beans 2 parts, extractum carnis 1 part, magnesium sulfate heptahydrate 0.3 part, potassium primary phosphate 0.3 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 5.5, load culturing bottle, 400 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 50KPa after 1 hour, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
The preparation method of pilose antler mushroom liquid strain, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10 parts, dregs of beans 2 parts, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3 part, potassium primary phosphate 0.3 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 5.5, load culturing bottle, 400 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 50KPa after 1 hour, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
(4), at 15 DEG C cool, after cooling, send into every bottle graft original seed 40;
(5), 20 DEG C of culturing room, humidity 70%, between gas concentration lwevel 1600PPM, cultivates 20 days.
Embodiment two
A kind of pilose antler mushroom liquid nutrient medium, comprises sucrose 30 parts, dregs of beans 5 parts, extractum carnis 3 parts, magnesium sulfate heptahydrate 1 part, potassium primary phosphate 1 part, defoamer 0.3 part by mass fraction.
The preparation method of pilose antler mushroom liquid nutrient medium, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 30 parts, dregs of beans 5 parts, extractum carnis 3 parts, magnesium sulfate heptahydrate 1 part, potassium primary phosphate 1 part, defoamer 0.3 part.；
(2), by step (1) raw material mix, control pH value 6.5, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 80KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
The preparation method of pilose antler mushroom liquid strain, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 30 parts, dregs of beans 5 parts, extractum carnis 3 parts, magnesium sulfate heptahydrate 1 part, potassium primary phosphate 1 part, defoamer 0.3 part.；
(2), by step (1) raw material mix, control pH value 6.5, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 80KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
(4), at 20 DEG C cool, after cooling, send into every bottle graft original seed 50;
(5), 25 DEG C of culturing room, humidity 80%, between gas concentration lwevel 3200PPM, cultivates 25 days.
Embodiment three
A kind of pilose antler mushroom liquid nutrient medium, comprises sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part by mass fraction.
A preparation method for pilose antler mushroom liquid nutrient medium, is characterized in that, comprise the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 6, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 70KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
A preparation method for pilose antler mushroom liquid strain, is characterized in that, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 6, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 70KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
(4), at 18 DEG C cool, after cooling, send into every bottle graft original seed 45;
(5), 22 DEG C of culturing room, humidity 75%, between gas concentration lwevel 2200PPM, cultivates 22 days.
Claims (2)
1. a preparation method for pilose antler mushroom liquid strain, is characterized in that, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part;
(2), by step (1) raw material mix, control pH value 5.5-6.5, load culturing bottle, every bottle of 400-500 part, gland encapsulates;
(3), culturing bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, insulation 30 minutes at 115 DEG C, insulation 60 minutes at 121 DEG C.
2.(4), at 15-20 DEG C cool, after cooling, send into every bottle graft original seed 40-50;
(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivates 20-25 days.
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| CN201410700133.XA CN104450539A (en) | 2014-11-28 | 2014-11-28 | Preparation method of velvet antler mushroom liquid strain |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104355915A (en) * | 2014-11-28 | 2015-02-18 | 江苏菇本堂生物科技股份有限公司 | Preparation method of liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. |
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
| CN112125732A (en) * | 2020-09-19 | 2020-12-25 | 江苏丰收菇业有限公司 | Velvet antler mushroom liquid stock culture solution and velvet antler mushroom stock preparation process |
Citations (4)
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| CN102907252A (en) * | 2011-08-05 | 2013-02-06 | 魏生龙 | Pilot-scale test fermentation process of mycelia of lyophyllum decastes |
| CN103011945A (en) * | 2012-10-16 | 2013-04-03 | 上海丰科生物科技股份有限公司 | Lyophyllum decastes solid strain medium formula and strain production method |
| CN104355915A (en) * | 2014-11-28 | 2015-02-18 | 江苏菇本堂生物科技股份有限公司 | Preparation method of liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. |
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
-
2014
- 2014-11-28 CN CN201410700133.XA patent/CN104450539A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907252A (en) * | 2011-08-05 | 2013-02-06 | 魏生龙 | Pilot-scale test fermentation process of mycelia of lyophyllum decastes |
| CN103011945A (en) * | 2012-10-16 | 2013-04-03 | 上海丰科生物科技股份有限公司 | Lyophyllum decastes solid strain medium formula and strain production method |
| CN104355915A (en) * | 2014-11-28 | 2015-02-18 | 江苏菇本堂生物科技股份有限公司 | Preparation method of liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. |
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
Non-Patent Citations (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104355915A (en) * | 2014-11-28 | 2015-02-18 | 江苏菇本堂生物科技股份有限公司 | Preparation method of liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. |
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
| CN112125732A (en) * | 2020-09-19 | 2020-12-25 | 江苏丰收菇业有限公司 | Velvet antler mushroom liquid stock culture solution and velvet antler mushroom stock preparation process |
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Application publication date: 20150325 |