CN113412760A - Hypsizigus marmoreus culture medium and hypsizigus marmoreus cultivation process - Google Patents
Hypsizigus marmoreus culture medium and hypsizigus marmoreus cultivation process Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention discloses a hypsizigus marmoreus culture medium and a hypsizigus marmoreus cultivation process, which comprise the following raw materials in percentage by weight: 20.6% of corncob, 5.3% of cottonseed hull, 10.9% of bran, 10.9% of rice bran, 3.2% of corn flour, 3.2% of soybean hull, 1.2% of beet pulp, 3.8% of bamboo sawdust, 2.1% of nutrient soil, 0.6% of calcium hydroxide, 38.2% of wood chip, and the beneficial effects are that: the germination speed of hyphae is accelerated through formula optimization; stabilizing the structure of the culture medium and keeping the homogeneity of the culture medium; the hypha spreading speed is accelerated, and the culture period is shortened; improve the biotransformation rate of the cultivation bottle and increase the production benefit.
Description
Technical Field
The invention relates to the technical field of edible fungi, in particular to a hypsizigus marmoreus culture medium and a hypsizigus marmoreus cultivation process.
Background
Hypsizigus marmoreus, the academic name Hypsizigus marmoreus, belonging to the subphylum Basidiomycotina, class Agaricales, order Agaricales, family Tricholomataceae, genus Hypsizigus, also known as Hypsizigus marmoreus, Hypsizygus marmoreus, and the like, is a large fungus which is saprophytic on wooden substrates. In natural environment, generally, autumn grows on dead or living hardwood trees such as beech.
At present, the hypsizigus marmoreus industrial production is a cultivation formula which mainly uses sawdust, cottonseed hulls and corncobs as main materials and uses rice bran, bran and soybean hulls as nutrient materials, and the defects of the existing cultivation technology are that hypha spreading speed is slow, cultivation period is long, growth vigor difference in the same batch in the hypha growth process is large, so that the later period fruiting biotransformation rate level is uneven, and enterprise production benefits are influenced.
Disclosure of Invention
Technical problem to be solved
The technical problem to be solved by the invention is as follows: at present, the hypsizigus marmoreus industrial production is a cultivation formula which mainly uses sawdust, cottonseed hulls and corncobs as main materials and uses rice bran, bran and soybean hulls as nutrient materials, and the defects of the existing cultivation technology are that hypha spreading speed is slow, cultivation period is long, growth vigor difference in the same batch in the hypha growth process is large, so that the later period fruiting biotransformation rate level is uneven, and enterprise production benefits are influenced.
(II) technical scheme
In order to overcome the problems that the existing hypsizigus marmoreus industrial production mainly uses sawdust, cottonseed hulls and corncobs as main materials and rice bran, bran and soybean hulls as nutrient materials, and the existing cultivation technology has the defects that the hypha spreading speed is low, the cultivation period is long, the growth vigor difference in the same batch in the hypha growth process is large, the later fruiting biotransformation rate level is uneven, and the enterprise production benefit is influenced, the invention provides a hypsizigus marmoreus culture medium and a hypsizigus marmoreus cultivation process, and the following technical scheme is adopted:
the culture medium for hypsizigus marmoreus comprises the following raw materials in percentage by weight: 20.6% of corncob, 5.3% of cottonseed hull, 10.9% of bran, 10.9% of rice bran, 3.2% of corn flour, 3.2% of soybean hull, 1.2% of beet pulp, 3.8% of bamboo sawdust, 2.1% of nutrient soil, 0.6% of calcium hydroxide and 38.2% of wood chip.
Furthermore, the using amount of the corncobs is 829kg, and the water content is 109.99 kg; the amount of the cottonseed hull is 213kg, and the water content is 28.18 kg; the amount of the bran is 438kg, and the water content is 73.82 kg; the usage amount of the rice bran is 438kg, and the water content is 60.24 kg; the using amount of the corn flour is 130kg, and the water content is 19.08 kg; the soybean hull consumption is 130kg, and the water content is 14.30 kg; the amount of beet residue is 47kg, and the water content is 4.85 kg; the bamboo dust consumption is 154kg, the water content is 76.97 kg; the dosage of the nutrient soil is 83kg, and the water content is 23.20 kg; the dosage of the calcium hydroxide is 24kg, and the water content is 0.39 kg; the amount of wood chips was 1539kg and the water content was 1126.65 kg.
A hypsizigus marmoreus cultivation process comprises the following steps:
s1: raw material selection and pretreatment
(1) Wood chip pretreatment: sieving sawdust, spraying water, sequentially performing primary stacking, primary stack turning, water spraying secondary stack turning, secondary stacking, water spraying tertiary stack turning, tertiary stacking, water spraying quaternary stack turning and quaternary stacking, and finally reserving for later use;
(2) bamboo chip pretreatment: crushing and sieving bamboo scraps, and drying for later use to ensure that the water content is not more than 60%;
(3) pretreatment of corncobs: crushing and sieving corncobs, and drying for later use to ensure that the water content is not more than 14%;
(4) pretreatment of cottonseed hulls: sieving cottonseed hull, and oven drying to ensure water content not more than 14%;
(5) pretreating beet pulp: crushing and sieving the beet pulp, and drying for later use, wherein the water content is ensured to be not more than 13%;
(6) corn flour pretreatment: crushing and sieving corns, and drying for later use, wherein the water content is not more than 16%;
(7) pretreatment of bran: sieving bran, and oven drying to ensure water content not greater than 18%;
(8) rice bran pretreatment: sieving rice bran, and oven drying to ensure water content not more than 15%;
(9) pretreating soybean hulls: crushing and sieving the soybean hulls, and then drying the soybean hulls for later use, wherein the water content is ensured to be not more than 13%;
(10) nutrient soil and calcium hydroxide: the water content is less than or equal to 1 percent, no caking is caused, and the fertilizer can be directly put into use;
s2: ingredient stirring
Sequentially putting wood chips, bamboo chips, corncobs, cottonseed hulls, beet pulp, bran, rice bran, soybean hulls, culture soil and calcium hydroxide into a stirring kettle with a spraying function, dry-mixing, adding water while stirring, and continuing stirring after adding water;
s3: bottling
Bottling the stirred culture medium by using a bottling machine, wherein each bottle is 1050-;
s4: sterilization
And pushing the filled bottles into a sterilization pot, putting the bottles orderly according to the positions, heating and sterilizing the bottles, vacuumizing the bottles twice, preserving the heat at 106 ℃ for 90 minutes, preserving the heat at 116 ℃ for 30 minutes, preserving the heat at 123 ℃ for 80 minutes, and stewing the bottles for 10 minutes. Exhausting to 0.004MPa after the stewing is finished;
s5: cooling down
When the temperature is not lower than 85 ℃, forcibly cooling the sterilized bacteria bottle in a doctor's advice cooling room to ensure that the temperature of the materials before inoculation is 18-22 ℃;
s6: inoculation of
Inoculating the fungus bottles in a sterile edible fungus inoculating machine;
s7: hypha culture
Transferring the inoculated strain bottle into a culture chamber for culture;
s8: scratching of fungi and growing of mushrooms
Adopting a ring mycelium stimulation mode, ensuring that the depth of a ring ditch is 1.8-2.0cm, the diameter of the steamed bun is 3.6-3.8cm and the water injection amount is 18-20ml after mycelium stimulation, and putting mycelium stimulation bottles into a culture room for culture;
s9: harvesting
And (4) harvesting the mature mushrooms.
Further, in step S2, the dry mixing time is 20 minutes, the continuous stirring time after the water addition is completed is 60 minutes, and the total stirring time is 100-150 minutes.
Further, in step S3, the frequency of cleaning the stirring rod of the bottle filling machine is 1 pan for 1 time, and the frequency of cleaning the punching rod is 5 baskets for 1 time.
Further, in step S6, the specific steps are as follows:
(1) cleaning and disinfecting the strain tank, the guide groove, the baffle plate and the strain bottle supporting plate by using 250ppm TCCCA solution;
(2) using an alcohol watering can to perform flame disinfection on each cleaned and disinfected part, wherein the flame disinfection of the metal part is not less than 5 seconds;
(3) burning and disinfecting the cutter head by flame of a gas burner, wherein each time is not less than 10 seconds;
(4) the compressed air valve is opened. Placing 2 strain bottles on the supporting plate, switching off an ultraviolet lamp switch, and switching on an illuminating lamp switch;
(5) and placing the treated strain bottle on a strain bottle supporting plate sterilized by 75% alcohol. Confirming the inoculation amount, and starting the inoculation operation;
(6) and strain bottles are timely and additionally placed on the standby strain bottle supporting plate, so that the continuous operation of inoculation is ensured.
Further, in step S6, the parts of the inoculating machine are cleaned and disinfected with 250ppm tcca solution every 30 minutes during the inoculation process, the parts are wiped clean, the cleaned and disinfected parts are flame-disinfected with an alcohol spray can, the flame disinfection of the metal parts cannot be less than 5 seconds, and the cutter head is burned and disinfected with a gas burner flame, each time is not less than 10 seconds.
Further, in step S7, the bacteria bottle is moved into a first-stage culture chamber with the temperature of 16-18 ℃, the carbon dioxide concentration controlled at 2000-4000ppm, the natural humidification is carried out, the culture period is 10-12 days, and then the bacteria bottle is moved into a second-stage culture chamber with the temperature of 17-19 ℃, the humidity controlled at 55-65%, the carbon dioxide concentration controlled at 3000-4500ppm, and the inter-bottle temperature after 40 days is 24.5-26.5 ℃; the humidity is controlled to be 55-65%; the concentration of carbon dioxide is controlled at 3500-5000ppm without illumination, the culture time is 90-115 days to ensure that the water content is detected by 70-72 percent and the pH value is 5.0-5.5.
(III) advantageous effects
The invention has the beneficial effects that:
(1) the germination speed of hyphae is accelerated through formula optimization;
(2) stabilize the structure of the culture medium and keep the uniformity of the culture medium.
(3) Accelerate the hypha spreading speed and shorten the culture period.
(4) Improve the biotransformation rate of the cultivation bottle and increase the production benefit.
Detailed Description
The present invention will now be described more fully hereinafter with reference to the accompanying examples, in which some, but not all examples of the invention are shown. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
the culture medium for hypsizigus marmoreus comprises the following raw materials in percentage by weight: 20.6% of corncob, 5.3% of cottonseed hull, 10.9% of bran, 10.9% of rice bran, 3.2% of corn flour, 3.2% of soybean hull, 1.2% of beet pulp, 3.8% of bamboo sawdust, 2.1% of nutrient soil, 0.6% of calcium hydroxide and 38.2% of wood chip.
Furthermore, the using amount of the corncobs is 829kg, and the water content is 109.99 kg; the amount of the cottonseed hull is 213kg, and the water content is 28.18 kg; the amount of the bran is 438kg, and the water content is 73.82 kg; the usage amount of the rice bran is 438kg, and the water content is 60.24 kg; the using amount of the corn flour is 130kg, and the water content is 19.08 kg; the soybean hull consumption is 130kg, and the water content is 14.30 kg; the amount of beet residue is 47kg, and the water content is 4.85 kg; the bamboo dust consumption is 154kg, the water content is 76.97 kg; the dosage of the nutrient soil is 83kg, and the water content is 23.20 kg; the dosage of the calcium hydroxide is 24kg, and the water content is 0.39 kg; the amount of wood chips was 1539kg and the water content was 1126.65 kg.
Meanwhile, a formula without bamboo dust is selected as a control group, and the formula comprises the following specific components: the using amount of the corncobs is 829kg, and the water content is 109.99 kg; the amount of the cottonseed hull is 213kg, and the water content is 28.18 kg; the amount of the bran is 438kg, and the water content is 73.82 kg; the usage amount of the rice bran is 438kg, and the water content is 60.24 kg; the using amount of the corn flour is 130kg, and the water content is 19.08 kg; the soybean hull consumption is 130kg, and the water content is 14.30 kg; the amount of beet residue is 47kg, and the water content is 4.85 kg; the dosage of the nutrient soil is 83kg, and the water content is 23.20 kg; the dosage of the calcium hydroxide is 24kg, and the water content is 0.39 kg; the amount of wood chips was 1693kg and the water content was 1239.11 kg.
Table 1: comparison of examples with control Components
A hypsizigus marmoreus cultivation process comprises the following steps:
s1 raw material selection and pretreatment
Wood chip: the sawdust is kept away from and is sieved water drenched, the primary stacking is carried out, the primary stacking (6 hours are sprayed every day during the primary stacking), the water drenches the secondary stacking, the secondary stacking (3 hours are sprayed every day during the secondary stacking), the water drenches the tertiary stacking for turning, the tertiary stacking (3 hours are sprayed every day during the tertiary stacking), the water drenches the quartic stacking for turning, the quartic stacking (3 hours are sprayed every day during the tertiary stacking), the standby (2 hours are sprayed every day during the standby), so that the sawdust raw material can be fully swelled, and the later-period soaking efficiency is better.
Bamboo dust: is yellow, has no mildew or peculiar smell, is crushed, sieved and dried for later use, has the water content of less than or equal to 60 percent, and can be directly put into use.
Corncob: no mildew, no peculiar smell and no heat, is crushed, sieved and dried for standby application, has the water content less than or equal to 14 percent and can be directly put into use.
Cotton seed hulls: no mildew, no peculiar smell and no heat, is crushed, sieved and dried for standby application, has the water content less than or equal to 14 percent and can be directly put into use.
Beet pulp: no mildew, no peculiar smell and no heat, crushing, sieving, drying for later use, wherein the water content is less than or equal to 13 percent, and crushing can be directly put into use.
Corn flour: it is bright yellow, has no mildew, odor and heat, is screened and dried for later use, has a water content of less than or equal to 16 percent, and can be directly put into use after being crushed.
Bran: no mildew, no peculiar smell and no heat, and is dried for standby after being screened, the water content is less than or equal to 18 percent, and the powder can be directly put into use.
Rice bran: no mildew, no peculiar smell and no heat, and is dried for standby after being screened, the water content is less than or equal to 15 percent, and the powder can be directly put into use.
Soybean hull: no mildew, no peculiar smell and no heat, is crushed, sieved and dried for standby application, has the water content less than or equal to 13 percent and can be directly put into use.
Nutrient soil and calcium hydroxide: the water content is less than or equal to 1 percent, no caking is caused, and the fertilizer can be directly put into use.
S2: ingredient stirring
Feeding materials according to the granularity of the raw materials in sequence to ensure that the materials are stirred more fully and uniformly after being fed; the specific feeding sequence is as follows: wood dust, bamboo dust, corncobs, cottonseed hulls, beet pulp, bran, rice bran, soybean hulls, culture soil and calcium hydroxide.
And pouring the weighed raw materials into a stirring pot in sequence, performing dry stirring for 20 minutes, adding water while stirring, continuing to stir for 60 minutes after the water is added, and waiting for discharging. The total stirring time is controlled at 100-150 minutes.
S3: bottling
Starting the bottle filling machine, selecting 1400cc of bacteria bottles, 6600 bottles/stirring, and firstly filling 10 baskets for initial inspection. The standard weight of each bottle is 1050-.
S4: sterilization
And pushing the filled bottles into a sterilization pot, putting the bottles orderly according to positions, starting an automatic sterilization program, vacuumizing twice, preserving heat at 106 ℃ for 90 minutes, preserving heat at 116 ℃ for 30 minutes, preserving heat at 123 ℃ for 80 minutes, and stewing for 10 minutes. And after the stewing is finished, exhausting to 0.004MPa, and opening the furnace door for exhausting. The total sterilization time is controlled within 5 hours.
S5: cooling down
When the temperature is not lower than 85 ℃, discharging, and moving the cultivation bottle to a cooling chamber for forced cooling. Ensuring that the temperature of the material before inoculation is 18-22 ℃, the water content is 63% -64.5%, the pH value is 5.8-6.2, the sugar degree is 10-15, and the average water loss is 10-30 g.
S6: inoculation of
And (3) cleaning and disinfecting the strain tank, the guide groove, the baffle plate and the strain bottle supporting plate by using 250ppm TCCCA solution. And (3) sterilizing the cleaned and sterilized components by flame, igniting 75% alcohol sprayed from an alcohol spray can by a gas burner, sterilizing the metal parts by flame for not less than 5 seconds, and sequentially installing the components after sterilizing by flame. The cutter head is burnt and disinfected by flame of a gas burner, and each time is not less than 10 seconds.
The compressed air valve is opened. Placing 2 strain bottles on a supporting plate, switching on a power switch, switching off an ultraviolet lamp switch, switching on an illuminating lamp switch, placing the treated strain bottles on the strain bottle supporting plate sterilized by 75% alcohol, confirming the inoculation amount, starting inoculation operation, timely supplementing and placing strain bottles on a standby strain bottle supporting plate, and ensuring that the inoculation operation is continuously carried out. When the strain bottle is placed additionally, the strain bottle supporting plate and the strain bottle are disinfected by spraying 75% alcohol, and the hand is soaked in 250ppm TCCCA solution for disinfection.
The inoculation part is cleaned and disinfected by 250ppm CCA solution every 30 minutes and wiped clean during inoculation. And (3) sterilizing the cleaned and sterilized components by flame, igniting 75% alcohol sprayed from an alcohol spray can by a gas burner, sterilizing the metal parts by flame for not less than 5 seconds, and sequentially installing the components after sterilizing by flame. The cutter head is burnt and disinfected by flame of a gas burner, and each time is not less than 10 seconds.
S7: hypha culture
After inoculation is finished, covering a moisture-proof bag by a basket-moving operator, moving a cultivation bottle into a first-stage cultivation room by a forklift worker in the cultivation room for cultivation, keeping the first-stage cultivation room clean for 10-12 days, controlling the space temperature of the first-stage cultivation room at 16-18 ℃, thus ensuring that the temperature between the bottles is controlled at 18-21 ℃, controlling the carbon dioxide concentration at 4000ppm, humidifying naturally, facilitating the rapid germination and field planting of hyphae, and moving the hyphae into a middle-stage cultivation after cultivation for 10-12 days; the temperature of the culture space of the second-stage culture room is controlled to be 17-19 ℃, because the hyphae can generate heat in the growth process, and the stacking density of the culture bottles is high, and the temperature in the material is higher than room temperature, the temperature of the spawn running center is controlled to be lower than 24 ℃ when the temperature of the culture space of the second-stage culture room is controlled to be 17-19 ℃, the temperature between the bottles is controlled to be 20-24 ℃ when the culture is carried out for 10-40 days, the humidity is controlled to be 55-65%, the concentration of carbon dioxide is controlled to be 4500ppm when the temperature between the bottles is 24.5-26.5 ℃ after 40 days; the humidity is controlled to be 55-65%; the concentration of carbon dioxide is controlled to 3500-5000ppm, no illumination is needed, and the opening of the internal circulation fan at the opening of 5m/1min is ensured to ensure the temperature, the humidity and the oxygen of the culture room to be uniform. The culture time is 90-115 days, the mycelium stimulation requirement is met, the water content is 70-72%, the pH value is 5.0-5.5, and mycelium stimulation is not forbidden.
S8: scratching of fungi and growing of mushrooms
Scratching requirements: by adopting a ring mushroom removing mode, the steamed bun is round after mushroom removing, the depth of a ring groove is 1.8-2.0cm, the diameter of the steamed bun is 3.6-3.8cm, and the water injection amount is 18-20 ml.
And (3) a fruiting stage of growth: the hyphae sequentially pass through a recovery period, a germination period, an inhibition period, a growth period and a harvesting period.
S9: harvesting
And (4) mushroom harvesting standard: the length is 12-14cm, the diameter of the mushroom cap is 1.8-2.0cm, the mushroom stem is long and straight, the mushroom cap is round, thick, solid and tough, and the mushroom is beautiful in the whole flower shape. At the same time, samples were taken randomly and weighed, and yield and grade were recorded.
Table 2: comparison of hyphal growth
Table 3: manifestation of fruiting stage
| Category of formulation | Concentration of hypha (+) | Mushroom stem | Mushroom cap |
| Examples | +++ | Strong, uniform and long | Round, thick and tough |
| Control group | ++ | Fine, uneven length, long and straight | Round, thin and low toughness |
Table 4: comparison of harvested data
| Category of formulation | Average yield per gram per bottle | Dry material in bottle weight/g | Bioconversion rate/% |
| Examples | 383 | 377.0 | 101.59 |
| Control group | 369 | 371.6 | 99.30 |
As can be seen from the comparison results, compared with the control group, the bottle full-bottle time of the hyphae of the bottle of the example is advanced by 5 days, the hyphae culture time is shortened, the production cost is saved, and the hyphae are thick and have better growth uniformity; the hypsizigus marmoreus cultivated in the embodiment has good quality and high yield per bottle; the mushroom stem is thick and uniform, and the mushroom cap is round, thick and tough. The biotransformation efficiency of the example bottle is also obviously improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. A hypsizigus marmoreus culture medium is characterized in that: the raw materials are as follows by weight percent: 20.6% of corncob, 5.3% of cottonseed hull, 10.9% of bran, 10.9% of rice bran, 3.2% of corn flour, 3.2% of soybean hull, 1.2% of beet pulp, 3.8% of bamboo sawdust, 2.1% of nutrient soil, 0.6% of calcium hydroxide and 38.2% of wood chip.
2. The culture medium for hypsizigus marmoreus according to claim 1, wherein: the using amount of the corncobs is 829kg, and the water content is 109.99 kg; the amount of the cottonseed hull is 213kg, and the water content is 28.18 kg; the amount of the bran is 438kg, and the water content is 73.82 kg; the usage amount of the rice bran is 438kg, and the water content is 60.24 kg; the using amount of the corn flour is 130kg, and the water content is 19.08 kg; the soybean hull consumption is 130kg, and the water content is 14.30 kg; the amount of beet residue is 47kg, and the water content is 4.85 kg; the bamboo dust consumption is 154kg, the water content is 76.97 kg; the dosage of the nutrient soil is 83kg, and the water content is 23.20 kg; the dosage of the calcium hydroxide is 24kg, and the water content is 0.39 kg; the amount of wood chips was 1539kg and the water content was 1126.65 kg.
3. The process for cultivating hypsizigus marmoreus according to any one of claims 1-2, wherein: the method comprises the following steps:
s1: raw material selection and pretreatment
(1) Wood chip pretreatment: sieving sawdust, spraying water, sequentially performing primary stacking, primary stack turning, water spraying secondary stack turning, secondary stacking, water spraying tertiary stack turning, tertiary stacking, water spraying quaternary stack turning and quaternary stacking, and finally reserving for later use;
(2) bamboo chip pretreatment: crushing and sieving bamboo scraps, and drying for later use to ensure that the water content is not more than 60%;
(3) pretreatment of corncobs: crushing and sieving corncobs, and drying for later use to ensure that the water content is not more than 14%;
(4) pretreatment of cottonseed hulls: sieving cottonseed hull, and oven drying to ensure water content not more than 14%;
(5) pretreating beet pulp: crushing and sieving the beet pulp, and drying for later use, wherein the water content is ensured to be not more than 13%;
(6) corn flour pretreatment: crushing and sieving corns, and drying for later use, wherein the water content is not more than 16%;
(7) pretreatment of bran: sieving bran, and oven drying to ensure water content not greater than 18%;
(8) rice bran pretreatment: sieving rice bran, and oven drying to ensure water content not more than 15%;
(9) pretreating soybean hulls: crushing and sieving the soybean hulls, and then drying the soybean hulls for later use, wherein the water content is ensured to be not more than 13%;
(10) nutrient soil and calcium hydroxide: the water content is less than or equal to 1 percent, no caking is caused, and the fertilizer can be directly put into use;
s2: ingredient stirring
Sequentially putting wood chips, bamboo chips, corncobs, cottonseed hulls, beet pulp, bran, rice bran, soybean hulls, culture soil and calcium hydroxide into a stirring kettle with a spraying function, dry-mixing, adding water while stirring, and continuing stirring after adding water;
s3: bottling
Bottling the stirred culture medium by using a bottling machine, wherein each bottle is 1050-;
s4: sterilization
And pushing the filled bottles into a sterilization pot, putting the bottles orderly according to the positions, heating and sterilizing the bottles, vacuumizing the bottles twice, preserving the heat at 106 ℃ for 90 minutes, preserving the heat at 116 ℃ for 30 minutes, preserving the heat at 123 ℃ for 80 minutes, and stewing the bottles for 10 minutes. Exhausting to 0.004MPa after the stewing is finished;
s5: cooling down
When the temperature is not lower than 85 ℃, forcibly cooling the sterilized bacteria bottle in a doctor's advice cooling room to ensure that the temperature of the materials before inoculation is 18-22 ℃;
s6: inoculation of
Inoculating the fungus bottles in a sterile edible fungus inoculating machine;
s7: hypha culture
Transferring the inoculated strain bottle into a culture chamber for culture;
s8: scratching of fungi and growing of mushrooms
Adopting a ring mycelium stimulation mode, ensuring that the depth of a ring ditch is 1.8-2.0cm, the diameter of the steamed bun is 3.6-3.8cm and the water injection amount is 18-20ml after mycelium stimulation, and putting mycelium stimulation bottles into a culture room for culture;
s9: harvesting
And (4) harvesting the mature mushrooms.
4. The hypsizigus marmoreus cultivation process according to claim 3, wherein: in the step S2, the dry mixing time is 20 minutes, the continuous stirring time after the water addition is completed is 60 minutes, and the total stirring time is 100-150 minutes.
5. The hypsizigus marmoreus cultivation process according to claim 3, wherein: in the step S3, the frequency of cleaning the stirring rod of the bottle filling machine is 1 pan for 1 time, and the frequency of cleaning the punching rod is 5 baskets for 1 time.
6. The hypsizigus marmoreus cultivation process according to claim 3, wherein: in step S6, the specific steps are as follows:
(1) cleaning and disinfecting the strain tank, the guide groove, the baffle plate and the strain bottle supporting plate by using 250ppm TCCCA solution;
(2) using an alcohol watering can to perform flame disinfection on each cleaned and disinfected part, wherein the flame disinfection of the metal part is not less than 5 seconds;
(3) burning and disinfecting the cutter head by flame of a gas burner, wherein each time is not less than 10 seconds;
(4) the compressed air valve is opened. Placing 2 strain bottles on the supporting plate, switching off an ultraviolet lamp switch, and switching on an illuminating lamp switch;
(5) and placing the treated strain bottle on a strain bottle supporting plate sterilized by 75% alcohol. Confirming the inoculation amount, and starting the inoculation operation;
(6) and strain bottles are timely and additionally placed on the standby strain bottle supporting plate, so that the continuous operation of inoculation is ensured.
7. The hypsizigus marmoreus cultivation process according to claim 6, wherein: in the step S6, the parts of the inoculation machine are cleaned and disinfected by 250ppm CCA solution every 30 minutes in the inoculation process, the parts are cleaned, an alcohol spray can is used for carrying out flame disinfection on the cleaned and disinfected parts, the flame disinfection of metal parts cannot be less than 5 seconds, and the cutter head is burnt by flame of a gas burner for disinfection, and each time is not less than 10 seconds.
8. The hypsizigus marmoreus cultivation process according to claim 3, wherein: in the step S7, the bacteria bottle is firstly moved into a first-stage culture chamber, the temperature of the first-stage culture chamber is 16-18 ℃, the concentration of carbon dioxide is controlled at 2000-4000ppm, natural humidification is carried out, the culture period is 10-12 days, then the bacteria bottle is moved into a second-stage culture chamber, the temperature of the second-stage culture chamber is 17-19 ℃, the humidity is 55-65%, the concentration of carbon dioxide is controlled at 3000-4500ppm, and the inter-bottle temperature is 24.5-26.5 ℃ after 40 days; the humidity is controlled to be 55-65%; the concentration of carbon dioxide is controlled at 3500-5000ppm without illumination, the culture time is 90-115 days to ensure that the water content is detected by 70-72 percent and the pH value is 5.0-5.5.
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