CN109006182A - A kind of culture base-material and preparation method thereof with Lenlinus edodes slag for cultivating oyster mushroom - Google Patents
A kind of culture base-material and preparation method thereof with Lenlinus edodes slag for cultivating oyster mushroom Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention provides a kind of culture base-material and preparation method thereof with Lenlinus edodes slag for cultivating oyster mushroom, is prepared by following component: plant base-material, Pond Silt, charcoal powder, urea, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash, fermentation auxiliary material, complex enzyme, saccharomycete, pH adjusting agent and deionized water.Pollution The present invention reduces mushroom bacteria residue to environment significantly improves the yield and quality of oyster mushroom, and preparation method is simple, raw material are easy to get, cost is relatively low.
Description
Technical field
The invention belongs to culture medium of edible fungus material technical fields, and in particular to a kind of culture with Lenlinus edodes slag for cultivating oyster mushroom
Base-material and preparation method thereof.
Background technique
Mushroom also known as mushroom, fragrant letter, fragrant bacterium, dried mushroom, fragrant wild rice, are the fructification of Pleurotaceae plant mushroom.Mushroom is the world
One of the second largest edible mushroom and China's specialty, in the civil title for being known as " mountain delicacy ".It is it is a kind of be grown in it is true on timber
Bacterium.Delicious flavour, fragrance make people mentally refreshing are full of nutrition.Mushroom is mild-natured rich in vitamin B complex, iron, potassium, vitamin D, sweet in flavor.It cures mainly
Appetite stimulator, few gas are out of strength；Mushroom is known as the title of " king of mountain delicacy ", is the nutritional health food of high protein, low fat.China is gone through
There is famous discussion to mushroom for physician.Modern medicine and nutrition deepen continuously research, and the medical value of mushroom is also continuous
It is exploited.Quantitative Determination of Ergosterol is very high in mushroom, effective to prevention and treatment rickets；Lentinan can enhance cell immunocompetent, from
And inhibit the growth of cancer cell；Mushroom contains more than 40 kinds of enzymes of six big enzymes, can correct human chitinase deficiency disease；Rouge in mushroom
It is beneficial to reduce blood lipid to human body for fat institute fatty acids.
With the production-scale continuous expansion of factory edible fungi, largely protein also rich in, cellulose are generated
With the bacteria residue of lignin and other nutritional ingredients, but China is also very low to the utilization rate of bacteria residue at present, causes the organic money of agricultural
The huge waste in source, some also cause new environmental pollution.Therefore, how bacteria residue is effectively handled in environmental protection well, it has also become one
Increasingly severeer problem.
Contain the nutrition such as a large amount of mycoprotein, amino acid, cellulose, hemicellulose and nitrogen, phosphorus, potassium in mushroom bacteria residue
Ingredient, it is full of nutrition, it is the ideal material for substituting straw.Continuous exploratory development discovery in recent years, can using edible fungi residue
It is feasible as dried mushroom culturing raw material, not only have it is low in cost, bacterium germination speed is fast, the purpose increased economic efficiency, meanwhile, be bacterium
The efficient circulation of slag using walking out a new way, with social city, technicalization, hommization development.
Notification number is CN101508599B, is disclosed a kind of using manioc waste and acid-sludge as raw material production high-quality edible mushroom culture
The method of base；By compounding manioc waste with the leftover bits and pieces science of two kinds of acid-sludge common fermentations or food industry, as production
High-quality edible mushroom culture medium, in the production that can be applied to all culture medium of edible fungus.Publication No. CN103880538A, it is open
A kind of culture base-material of hickory chick and preparation method thereof, culture base-material is made of following components: peat, soybean residue, pomelo peel,
Tomato seedling, plant ash, potassium dihydrogen phosphate, urea, land plaster, quick lime and regulator；The preparation method of culture base-material, including
Following steps: (1) it stocks up；(2) it crushes；(3) it mixes；(4) it ferments；(5) it charges.What the above prior art provided is with hickory chick
Culture base-material adds peat and a variety of wastes on the basis of conventional medium material, is also used for mycelia production.But sheep tripe
The saprophytic edible mushroom of Pseudomonas low temperature and high relative humidity type, the culture base-material of existing conventional cultivation hickory chick is at high cost, bacterium germination speed is slow, raw
Long period length, bacterium germination low output, adopt the mushroom phase it is short the disadvantages of, therefore be not appropriate for industrialization promotion production.But mushroom can be effective
Avoid disadvantage mentioned above, and play the role of preferably promoting mycelia to produce.
Summary of the invention
The object of the present invention is to provide a kind of culture base-material and preparation method thereof with Lenlinus edodes slag for cultivating oyster mushroom, the present invention
Reduce pollution of the mushroom bacteria residue to environment, significantly improve the yield and quality of oyster mushroom, and preparation method is simple, raw material are easy
It obtains, cost is relatively low.
The present invention provides the following technical solutions:
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: 15-20 parts of plant base-material, pond
Mud 6-10 parts of the pool, 11-15 parts of charcoal powder, 7-14 parts of urea, 15-19 parts of mushroom bacteria residue, 8-13 parts of agricultural crop straw, family's animal manure
Just 5-8 parts, 6-9 parts of plant ash, auxiliary material 3-7 parts of fermentation, 3-5 parts of complex enzyme, 4-6 parts of saccharomycete, 2-4 parts of pH adjusting agent and go
7-10 parts of ionized water.
Preferably, the culture base-material includes the raw material of following parts by weight: 17-20 parts of plant base-material, Pond Silt 6-9
Part, 13-15 parts of charcoal powder, 7-12 parts of urea, 17-19 parts of mushroom bacteria residue, 8-11 parts of agricultural crop straw, 6-8 parts of animal waste, grass
6-8 parts of wood ash, auxiliary material 4-7 parts of fermentation, 3-4 parts of complex enzyme, 5-6 parts of saccharomycete, 2-3 parts of pH adjusting agent and deionized water 7-9
Part.
Preferably, the culture base-material includes the raw material of following parts by weight: 19 parts of plant base-material, 8 parts of Pond Silt, charcoal
14 parts of powder, 9 parts of urea, 18 parts of mushroom bacteria residue, 10 parts of agricultural crop straw, 7 parts of animal waste, 6 parts of plant ash, fermentation 5 parts of auxiliary material,
3 parts of complex enzyme, 5 parts of saccharomycete, 2 parts of pH adjusting agent and 7 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, imports in blender, stirs 15- under the revolving speed of 200-240r/min
18min adds complex enzyme, and enzymolysis processing 0.8-1.2h is carried out under 45-48 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer, in 100-
It is stirred 20-25min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor,
Obtain fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 55-58 DEG C, adjusts water content in 65-68%, add pH adjusting agent, adjust
PH value is saved in 6.5-7, finished product can be obtained.
Preferably, the plant base-material of the step a is rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio mixing
It forms.
Preferably, the complex enzyme of the step a is that protease and cellulase 2:5 in mass ratio are mixed.
Preferably, the agricultural crop straw of the step b is that wheat stalk, rice straw, corn stover and rape stalk are pressed
Mass ratio 1:1:2:2 is mixed.
Preferably, the fermentation auxiliary material of the step b is calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio
It mixes.
Preferably, the method for the fermentation process of the step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust
Saving fermentation temperature is 38 DEG C, is fermented 4 days.
Preferably, the method for the sterilization treatment of the step c are as follows: in the case where pressure is 5MPa, sterilized using ultraviolet light
Handle 2h.
The beneficial effects of the present invention are:
Pollution The present invention reduces mushroom bacteria residue to environment significantly improves the yield and quality of oyster mushroom, and preparation method letter
List, raw material are easy to get, cost is relatively low.
Mushroom bacteria residue, agricultural crop straw and animal waste in the present invention, effectively realizing turns waste into wealth, and improves its benefit
With value, natural resources is saved, cost of the invention is reduced.
Under the synergistic effect of the nutritional ingredient other compositions contained in mushroom bacteria residue in the present invention, mycelia can be effectively improved
The speed of growth, improve its fruiting density, and make mycelia robust growth.
Plant base-material in the present invention is the proportion of rice husk, peanut shell, wheat bran and leaf, under the ratio, the above ingredient
It is from a wealth of sources, be easy to get with good nutrient raw material, and its benefit with waste utilization, the present invention greatly reduces
Cost.
Complex enzyme in the present invention is the proportion of protease and cellulase, under the sub ratio, is conducive to efficiently quickly
Decomposition plant base-material, refine its nutritional ingredient more, be conducive to quickly be absorbed by oyster mushroom, thus improve its growth speed
Degree.
The method of fermentation process in the present invention is conducive to improve fermentation efficiency, and makes fermentation more abundant, makes to seek
It forms and gets adequately release, consequently facilitating the absorption of oyster mushroom, also further increases the utilization rate of raw material.
Specific embodiment
Embodiment 1
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: 20 parts of plant base-material, pond are become silted up
10 parts of mud, 11 parts of charcoal powder, 7 parts of urea, 17 parts of mushroom bacteria residue, 8 parts of agricultural crop straw, 5 parts of animal waste, 6 parts of plant ash, hair
7 parts of ferment auxiliary material, 5 parts of complex enzyme, 6 parts of saccharomycete, 4 parts of pH adjusting agent and 10 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, is imported in blender, stir 15min under the revolving speed of 200r/min, then plus
Enter complex enzyme, enzymolysis processing 0.8h is carried out under 45 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer,
It is stirred 25min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor, obtains
Fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 58 DEG C, adjusts water content 68%, add pH adjusting agent, adjust pH value
7, finished product can be obtained.
The plant base-material of step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
The complex enzyme of step a is that protease and cellulase 2:5 in mass ratio are mixed.
The agricultural crop straw of step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2 in mass ratio:
2 mix.
The fermentation auxiliary material of step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
The method of the fermentation process of step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
The method of the sterilization treatment of step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
Embodiment 2
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: culture base-material includes following heavy
Measure the raw material of part: 20 parts of plant base-material, 6 parts of Pond Silt, 15 parts of charcoal powder, 7 parts of urea, 17 parts of mushroom bacteria residue, crops straw
11 parts of stalk, 6 parts of animal waste, 8 parts of plant ash, fermentation 7 parts of auxiliary material, 4 parts of complex enzyme, 5 parts of saccharomycete, 3 parts of pH adjusting agent and
7 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, is imported in blender, stir 15min under the revolving speed of 240r/min, then plus
Enter complex enzyme, enzymolysis processing 1.2h is carried out under 45 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer,
It is stirred 20min under the revolving speed of 100r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor, obtains
Fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 55 DEG C, adjusts water content 65%, add pH adjusting agent, adjust pH value
7, finished product can be obtained.
The plant base-material of step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
The complex enzyme of step a is that protease and cellulase 2:5 in mass ratio are mixed.
The agricultural crop straw of step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2 in mass ratio:
2 mix.
The fermentation auxiliary material of step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
The method of the fermentation process of step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
The method of the sterilization treatment of step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
Embodiment 3
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: culture base-material includes following heavy
Measure the raw material of part: 19 parts of plant base-material, 8 parts of Pond Silt, 14 parts of charcoal powder, 9 parts of urea, 18 parts of mushroom bacteria residue, crops straw
10 parts of stalk, 7 parts of animal waste, 6 parts of plant ash, fermentation 5 parts of auxiliary material, 3 parts of complex enzyme, 5 parts of saccharomycete, 2 parts of pH adjusting agent and
7 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, is imported in blender, stir 15min under the revolving speed of 200r/min, then plus
Enter complex enzyme, enzymolysis processing 1.2h is carried out under 48 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer,
It is stirred 20min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor, obtains
Fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 58 DEG C, adjusts water content 68%, add pH adjusting agent, adjust pH value
6.5, finished product can be obtained.
The plant base-material of step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
The complex enzyme of step a is that protease and cellulase 2:5 in mass ratio are mixed.
The agricultural crop straw of step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2 in mass ratio:
2 mix.
The fermentation auxiliary material of step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
The method of the fermentation process of step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
The method of the sterilization treatment of step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
Embodiment 4
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: 20 parts of plant base-material, pond are become silted up
10 parts of mud, 11 parts of charcoal powder, 7 parts of urea, 0 part of mushroom bacteria residue, 8 parts of agricultural crop straw, 5 parts of animal waste, 6 parts of plant ash, hair
7 parts of ferment auxiliary material, 5 parts of complex enzyme, 6 parts of saccharomycete, 4 parts of pH adjusting agent and 10 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, is imported in blender, stir 15min under the revolving speed of 200r/min, then plus
Enter complex enzyme, enzymolysis processing 0.8h is carried out under 45 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer,
It is stirred 25min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor, obtains
Fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 58 DEG C, adjusts water content 68%, add pH adjusting agent, adjust pH value
7, finished product can be obtained.
The plant base-material of step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
The complex enzyme of step a is that protease and cellulase 2:5 in mass ratio are mixed.
The agricultural crop straw of step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2 in mass ratio:
2 mix.
The fermentation auxiliary material of step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
The method of the fermentation process of step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
The method of the sterilization treatment of step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
Embodiment 5
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: 20 parts of plant base-material, pond are become silted up
10 parts of mud, 11 parts of charcoal powder, 7 parts of urea, 15 parts of mushroom bacteria residue, 8 parts of agricultural crop straw, 5 parts of animal waste, 6 parts of plant ash, hair
7 parts of ferment auxiliary material, 5 parts of complex enzyme, 6 parts of saccharomycete, 4 parts of pH adjusting agent and 10 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, is imported in blender, stir 15min under the revolving speed of 200r/min, then plus
Enter complex enzyme, enzymolysis processing 0.8h is carried out under 45 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer,
It is stirred 25min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor, obtains
Fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 58 DEG C, adjusts water content 68%, add pH adjusting agent, adjust pH value
7, finished product can be obtained.
The plant base-material of step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
The complex enzyme of step a is that protease and cellulase 2:5 in mass ratio are mixed.
The agricultural crop straw of step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2 in mass ratio:
2 mix.
The fermentation auxiliary material of step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
The method of the fermentation process of step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
The method of the sterilization treatment of step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
Embodiment 6
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: 20 parts of plant base-material, pond are become silted up
10 parts of mud, 11 parts of charcoal powder, 7 parts of urea, 18 parts of mushroom bacteria residue, 8 parts of agricultural crop straw, 5 parts of animal waste, 6 parts of plant ash, hair
7 parts of ferment auxiliary material, 5 parts of complex enzyme, 6 parts of saccharomycete, 4 parts of pH adjusting agent and 10 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, is imported in blender, stir 15min under the revolving speed of 200r/min, then plus
Enter complex enzyme, enzymolysis processing 0.8h is carried out under 45 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer,
It is stirred 25min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor, obtains
Fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 58 DEG C, adjusts water content 68%, add pH adjusting agent, adjust pH value
7, finished product can be obtained.
The plant base-material of step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
The complex enzyme of step a is that protease and cellulase 2:5 in mass ratio are mixed.
The agricultural crop straw of step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2 in mass ratio:
2 mix.
The fermentation auxiliary material of step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
The method of the fermentation process of step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
The method of the sterilization treatment of step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
Embodiment 7
A kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, the raw material including following parts by weight: 20 parts of plant base-material, pond are become silted up
10 parts of mud, 11 parts of charcoal powder, 7 parts of urea, 19 parts of mushroom bacteria residue, 8 parts of agricultural crop straw, 5 parts of animal waste, 6 parts of plant ash, hair
7 parts of ferment auxiliary material, 5 parts of complex enzyme, 6 parts of saccharomycete, 4 parts of pH adjusting agent and 10 parts of deionized water.
A kind of preparation method of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, including following preparation step:
A, plant base-material and deionized water are mixed, is imported in blender, stir 15min under the revolving speed of 200r/min, then plus
Enter complex enzyme, enzymolysis processing 0.8h is carried out under 45 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer,
It is stirred 25min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor, obtains
Fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 58 DEG C, adjusts water content 68%, add pH adjusting agent, adjust pH value
7, finished product can be obtained.
The plant base-material of step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
The complex enzyme of step a is that protease and cellulase 2:5 in mass ratio are mixed.
The agricultural crop straw of step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2 in mass ratio:
2 mix.
The fermentation auxiliary material of step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
The method of the fermentation process of step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
The method of the sterilization treatment of step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
Embodiment 4-7 is the adjustment to the single deal of mushroom bacteria residue carried out on that basis of example 1.
Comparative example 1
Detection comparison is carried out using common oyster mushroom culture medium material in the prior art.
The finished product for detecting above embodiments and comparative example preparation, obtains following detection data:
Table one:
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 |
| Hypha of Pleurotus ostreatus length (mm) after inoculation 5 days | 22 | 24 | 25 | 20 |
| Hypha of Pleurotus ostreatus length (mm) after inoculation 10 days | 75 | 75 | 78 | 66 |
| Hypha of Pleurotus ostreatus length (mm) after inoculation 15 days | 122 | 127 | 128 | 103 |
| Oyster mushroom is covered with the time (day) of 80% culture medium needs | 25 | 24 | 24 | 28 |
| Oyster mushroom is covered with the time (day) of 100% culture medium needs | 28 | 28 | 27 | 33 |
Table two:
| Detection project | 0 part of embodiment 4(mushroom bacteria residue content) | 15 parts of embodiment 5(mushroom bacteria residue content) | 18 parts of embodiment 6(mushroom bacteria residue content) | 19 parts of embodiment 7(mushroom bacteria residue content) |
| 5 balance mushroom Hyphal lengths (mm) | 11 | 22 | 25 | 22 |
| 10 balance mushroom Hyphal lengths (mm) | 38 | 76 | 77 | 75 |
| 15 balance mushroom Hyphal lengths (mm) | 70 | 122 | 126 | 124 |
| Oyster mushroom is covered with the time (day) of 80% culture medium needs | 36 | 25 | 25 | 25 |
| Oyster mushroom is covered with the time (day) of 100% culture medium needs | 45 | 28 | 28 | 28 |
By table one, the resulting experimental data of table two, it can be deduced that, the properties of the finished product of preparation method preparation of the invention are aobvious
Excellent in common product in the prior art, and the preferred preparation method in the embodiment of the present invention 3 and embodiment 6 is write,
The end properties that it is obtained are the most excellent.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom, which is characterized in that the raw material including following parts by weight: plant base
Expect 15-20 parts, 6-10 parts of Pond Silt, 11-15 parts of charcoal powder, 7-14 parts of urea, 15-19 parts of mushroom bacteria residue, agricultural crop straw
8-13 parts, 5-8 parts of animal waste, 6-9 parts of plant ash, fermentation auxiliary material 3-7 parts, 3-5 parts of complex enzyme, 4-6 parts of saccharomycete, pH value tune
Agent 2-4 parts and deionized water 7-10 parts of section.
2. a kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 1, which is characterized in that the culture
Base-material includes the raw material of following parts by weight: 17-20 parts of plant base-material, 6-9 parts of Pond Silt, 13-15 parts of charcoal powder, urea 7-12
Part, 17-19 parts of mushroom bacteria residue, 8-11 parts of agricultural crop straw, 6-8 parts of animal waste, 6-8 parts of plant ash, fermentation auxiliary material 4-7 parts,
3-4 parts of complex enzyme, 5-6 parts of saccharomycete, 2-3 parts of pH adjusting agent and 7-9 parts of deionized water.
3. a kind of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 1, which is characterized in that the culture
Base-material includes the raw material of following parts by weight: 19 parts of plant base-material, 8 parts of Pond Silt, 14 parts of charcoal powder, 9 parts of urea, mushroom bacteria residue
18 parts, 10 parts of agricultural crop straw, 7 parts of animal waste, 6 parts of plant ash, fermentation 5 parts of auxiliary material, 3 parts of complex enzyme, 5 parts of saccharomycete, pH
It is worth 2 parts and 7 parts of deionized water of regulator.
4. a kind of described in any item preparation methods of the culture base-material with Lenlinus edodes slag for cultivating oyster mushroom of claim 1-3, special
Sign is, including following preparation step:
A, plant base-material and deionized water are mixed, imports in blender, stirs 15- under the revolving speed of 200-240r/min
18min adds complex enzyme, and enzymolysis processing 0.8-1.2h is carried out under 45-48 DEG C of heating water bath, obtains enzymatic hydrolysis material；
B, mushroom bacteria residue, agricultural crop straw, animal waste, plant ash and fermentation auxiliary material are mixed, is imported in batch mixer, in 100-
It is stirred 20-25min under the revolving speed of 120r/min, then is imported together with saccharomycete and is sealed fermentation process in fermentor,
Obtain fermentation material；
C, enzymatic hydrolysis material, fermentation material, Pond Silt, charcoal powder and urea are mixed, after mixing evenly, carries out sterilization treatment, obtain
Sterilizing material；
D, sterilizing is expected to import in reaction kettle, at 55-58 DEG C, adjusts water content in 65-68%, add pH adjusting agent, adjust
PH value is saved in 6.5-7, finished product can be obtained.
5. a kind of preparation method of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 4, feature exist
In the plant base-material of the step a is that rice husk, peanut shell, wheat bran and leaf 8:4:5:3 in mass ratio are mixed.
6. a kind of preparation method of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 4, feature exist
In the complex enzyme of the step a is that protease and cellulase 2:5 in mass ratio are mixed.
7. a kind of preparation method of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 4, feature exist
In the agricultural crop straw of the step b is wheat stalk, rice straw, corn stover and rape stalk 1:1:2:2 in mass ratio
It mixes.
8. a kind of preparation method of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 4, feature exist
In the fermentation auxiliary material of the step b is that calcium superphosphate, potassium dihydrogen phosphate and magnesium sulfate 4:2:1 in mass ratio are mixed.
9. a kind of preparation method of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 4, feature exist
In the method for the fermentation process of the step b are as follows: ferment 5 days at 42 DEG C, carry out turning processing, then adjust fermentation temperature and be
It 38 DEG C, ferments 4 days.
10. a kind of preparation method of culture base-material with Lenlinus edodes slag for cultivating oyster mushroom according to claim 4, feature exist
In the method for the sterilization treatment of the step c are as follows: in the case where pressure is 5MPa, carry out sterilization treatment 2h using ultraviolet light.
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